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10229 ABS White Willow Bark Extract PowderEnhances Cell Renewal + Antioxidant + Antimicrobial
Product Code: 10229
INCI Name: Salix alba (White Willow) Bark Extract
INCI Status: Approved
Suggested Use Levels: 1.0 – 10.0%
Suggested Applications: Exfoliation, Great for Problem Skin, Antioxidant
Solubility: Water Soluble
• Traditionally used as a remedy for its anti-inflammatory and analgesic properties, willow bark extracts are employed as a source of natural salicylates.
• Salicylic acid has been identified as part of the willow tree’s endogenous defense mechanisms – protects against invading pathogens
• The bark of the white willow is a natural source of salicylic acid-like ingredients
• ABS White Willow Bark Extract Powder is standardized for 53-65% natural salicylates and provides formulators with the perfect alternative to synthetic salicylic acid products!
10229 ABS White Willow Bark Extract PowderNatural Alternative to Salicylic Acid
• ABS White Willow Bark Extract Powder is ideal for reducing inflammation and associated redness, plus, it acts as an efficacious antimicrobial.
• As reported at the Society of Investigative Dermatology, White Willow Bark Extracts provide the benefits of salicylic acid without any of the typically associated irritation usually found when using synthetic AHAs
• Water-solubility of ABS White Willow Bark Extract Powder promotes seamless incorporation into a variety of formulas promoting all of the benefits associated with salicylic acid and none of the irritation!
10229 ABS White Willow Bark Extract PowderEfficacy Without Irritation
10229 ABS White Willow Bark Extract PowderAvailable Efficacy
• In-vivo Efficacy Studies• Cellular Renewal Assay• High Resolution Ultrasound Skin Imaging Assay• Salon Half Head Study
• In-vitro Efficacy Studies• Challenge Test• Inhibition Activity vs. P. acnes and S. aureus• Oxygen Radical Absorbance Capacity Assay• IL-6 ELISA
10229 ABS White Willow Bark Extract Powderin-vivo Cellular Renewal Assay
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1% Synthetic Salicylic Acid 2% ABS White Willow Powder(in water)
control
Per
cen
t (%
) In
crea
se
Protocol
• 15 m/f volunteers
• Control: 5.0% Petroletum-Based DansylChloride Solution
• Test materials: 1.0% Synthetic Salicylic Acid (3.9 pH w/w) + 2.0% ABS White Willow Bark Extract Powder (equivalent to 1.0% Synthetic SA)
• UV light was used to determine the color intensity relative to the level of binding and percent increase in cellular renewal
• ABS White Willow Bark Extract Powder is capable of increasing cellular renewal
Figure 1. Increase in cellular renewal.
10229 ABS White Willow Bark Extract Powderin-vivo High Resolution Skin Imaging
Protocol
• Equipment: DermaLab Combo
• Principle of measurement: Conductance, single frequency
• Subjects: 10 (m/f)
• Test area: Volar forearms
• Concentration of active used: 2.0%
• Frequency of application: Twice Daily
• ABS White Willow Bark Extract Powder is capable of significantly improving skin density
Figure 2. Improvements in skin density following application of the test materials.
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t = 24 1 week 2 week 3 week 4 weekPer
cen
t (%
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iffe
ren
ce B
etw
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tes
Experimental vs.Untreated Control
Base Lotion vs.Untreated Control
Experimental vs.Base Lotion
10229 ABS White Willow Bark Extract Powderin-vitro Antimicrobial Assay – Zone of Inhibition
Protocol
• The zone of inhibition for 2.00% ABS White Willow Bark Extract Powder was determined in relation to the zones of inhibition for 1.00% Salicylic acid and Butylene glycol against both S. aureus and P. acnes.
• 2.00% ABS White Willow Bark Extract Powder is more effective than 1.00% Salicylic Acid in inhibiting the growth of both S. aureus and P. acnes.
Figure 3. Zone of inhibition data for S. aureus.
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1% Salicylic Acid 2% ABS White Willow BarkPowder
1% Butylene Glycol
Mill
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ers
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Zone of Inhibition – Staphylococcus aureus
10229 ABS White Willow Bark Extract Powderin-vitro Antimicrobial Assay – Zone of Inhibition
Protocol
• The zone of inhibition for 2.00% ABS White Willow Bark Extract Powder was determined in relation to the zones of inhibition for 1.00% Salicylic acid and Butylene glycol against both S. aureus and P. acnes.
• 2.00% ABS White Willow Bark Extract Powder is more effective than 1.00% Salicylic Acid in inhibiting the growth of both S. aureus and P. acnes.
Figure 4. Zone of inhibition data for P. acnes
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1% Salicylic Acid 2% ABS White Willow BarkPowder
1% Butylene Glycol
Mill
imet
ers
(mm
)
Zone of Inhibition - Propionibacterium acnes
10229 ABS White Willow Bark Extract PowderOxygen Radical Absorbance Capacity (ORAC) Assay
Protocol
• Trolox® was used as the positive control
• Test Quantity: 0.001% and 0.0005%
• Fluorescent measurements were taken every two minutes for two hours
• ABS White Willow Bark Extract Powder showed antioxidant activity at levels as low as 0.0005%
Figure 5. Antioxidant activity of test materials
198
13
186
88
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200µM Trolox 12.5µM Trolox 0.001% 10229 ABS WhiteWillow Bark Extract
Powder
0.0005% 10229 ABSWhite Willow Bark
Extract Powder
An
tio
xid
ant
Cap
acit
y (µ
MTE
)
10229 ABS White Willow Bark Extract Powderin-vitro IL-6 ELISA Assay
Protocol
• Human dermal fibroblasts were seeded into 12-well tissue culture plates
• Test materials were added to complete DMEM containing 1µg/mL LPS and incubated with fibroblasts for 24 hours
• Concentrations: 1%, 0.1%, 0.01%
• ABS White Willow Bark Extract Powder exhibited anti-inflammatory effects on LPS-treated fibroblasts
• The changes in IL-6 production using ABS White Willow Bark Extract Powder appear to be dose dependent
Figure 6. Reduction in IL-6 concentration
1086.64
2599.80
2859.78
2599.40
1423.38
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1.0% ABS WillowBark Extract
0.1% ABS WillowBark Extract
0.01% ABS WillowBark Extract
Positive Control Negative Control
IL-6
Co
nce
ntr
atio
n (
pg/
mL)
10229 ABS White Willow Bark Extract PowderEnhances Cell Renewal + Antioxidant + Antimicrobial
Product Code: 10229
INCI Name: Salix alba (White Willow) Bark Extract
INCI Status: Approved
Suggested Use Levels: 1.0 – 10.0%
Suggested Applications: Exfoliation, Great for Problem Skin, Antioxidant
Solubility: Water Soluble
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