1 Polymorphism and gene conversion of the 16S rRNA genes in the multiple rRNA operons of Vibrio pa rahaemolyticus Narjol Gonza’lez-Escalona, Jaime Romero, Romilio T. Espejo First published online 22 April 2005 Edited by J.M. Ketley Speaker 連連連 : Date : 94/12/13
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1 Polymorphism and gene conversion of the 16S rRNA genes in the multiple rRNA operons of Vibrio parahaemolyticus Narjol Gonza’lez-Escalona, Jaime Romero,
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Polymorphism and gene conversion of the 16S rRNA genes
in the multiple rRNA operons of Vibrio parahaemolyticus
Narjol Gonza’lez-Escalona, Jaime Romero, Romilio T. Espejo
First published online 22 April 2005Edited by J.M. Ketley
Speaker:連家翎Date: 94/12/13
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Introduction
Vibrio parahaemolyticus
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Introduction
Vibrio parahaemolyticus
chromosome 1 chromosome 2
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Introduction
Chromosome 1 Chromosome 2
Length of sequence: 3,288,558 bp 1,877,212 bp G+C ratio: 45.4 % 45.4 %
Open reading frame (ORF):
3,080 1,752
Protein coding region: 86.9 % 86.9 %
Average ORFs length: 926.9 bp 931.3 bp rRNA operons 10 1
tRNA: 112 14
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Introduction
• Difference of Vibrio. between 16S rRNA genes ( rrs )
• VpKX cotains almost identical rrs
• VpD cotains two rrs classes :– differing 10 nucleotide site of 25bp sequence– 11 rrn : 7 contain class1 , 4 contain class 2
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rrs of VpD and VpKX
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Introduction
• Gene conversion
– The possibility that two or more rrs might independently acquire the same sequence by randon mutation is zero