1 Biomaterials Evolution This platform will create massively- parallel capabilities for directed evolution of biomolecules, such as DNA, for regenerative medicine and bioenergy Wyss Faculty Lead: George Church Other Wyss Faculty: Pam Silver William Shih Jim Collins Don Ingber Overall Goals: • Directed Evolution of Human Antibodies • Multi-Gene Switches for Cell
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1 Biomaterials Evolution This platform will create massively-parallel capabilities for directed evolution of biomolecules, such as DNA, for regenerative.
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Biomaterials Evolution
This platform will create massively-parallel capabilities for directed evolution of biomolecules, such as DNA,
for regenerative medicine and bioenergy Wyss Faculty Lead: George Church
Other Wyss Faculty: Pam Silver William Shih Jim Collins Don Ingber
Overall Goals: • Directed Evolution of Human Antibodies • Multi-Gene Switches for Cell Reprogramming• DNA as a Cost Effective Biomaterial
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Biomaterials Evolution Platform
•DNA Synthesis, assembly & analysis on chips -DNA as a Cost Effective Biomaterial (Shih) -Human Antibody & Antigen Libraries (Ingber)•Directed Evolution of Microbial Communities -Metabolic engineering fuels, chemicals (Silver, Collins)•Gene Switches for Human Cell Reprogramming -Combinatorial Control of Stem Cell & Cancer Cell Differentiation (Ingber & Collins)
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Sequencing tracked Moore’s law 2X/2 yr until 2005-9 10,000x/4yr
2009 goal of 2% for $200 (coding + regulatory)
0.0000001
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0.1
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1990 1995 2000 2005 2010
$/bp 2005 $50M
2009 $5K
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Open-architecture hardware, software, wetware
Polonator
$150K - 2 billion beads/run
e.g.1981IBM PC
Rich TerrySenior Engineer
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DNA Synthesis, assembly & analysis on chips
Nitrobenzyl linkers for selective release of beads or cells
Digital micromirror & inkjet
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Biomaterials Evolution Platform
•DNA Synthesis, assembly & analysis on chips -DNA as a Cost Effective Biomaterial (Shih) -Human Antibody & Antigen Libraries (Ingber)•Directed Evolution of Microbial Communities -Metabolic engineering fuels, chemicals (Silver, Collins)•Gene Switches for Human Cell Reprogramming -Combinatorial Control of Stem Cell & Cancer Cell Differentiation (Ingber & Collins)