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1 A JPEG-LS Based Lossless/Lossy Compression Method for Two-Dimensional Electrophoresis Images Source: 2003 International Conference on Informatics, Cybernetics, and Systems Authors: Kevin I-J Ho, Tung-Shou Chen, Hui-Fang Tsai, Mingli Hsieh, and Chia-Chun Wu Speaker: Chia-Chun Wu ( 吳吳吳 ) Date: 2004/12/09
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1 A JPEG-LS Based Lossless/Lossy Compression Method for Two-Dimensional Electrophoresis Images Source: 2003 International Conference on Informatics, Cybernetics,

Jan 29, 2016

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Page 1: 1 A JPEG-LS Based Lossless/Lossy Compression Method for Two-Dimensional Electrophoresis Images Source: 2003 International Conference on Informatics, Cybernetics,

1

A JPEG-LS Based Lossless/Lossy Compression Method

for Two-Dimensional Electrophoresis Images

Source: 2003 International Conference on Informatics, Cybernetics, and Systems

Authors: Kevin I-J Ho, Tung-Shou Chen, Hui-Fang Tsai, Mingli Hsieh, and Chia-Chun Wu

Speaker: Chia-Chun Wu (吳佳駿 )Date: 2004/12/09

Page 2: 1 A JPEG-LS Based Lossless/Lossy Compression Method for Two-Dimensional Electrophoresis Images Source: 2003 International Conference on Informatics, Cybernetics,

2 NCHU

Outline

IntroductionSchemaCompression MethodDecompression MethodResultsConclusion

Page 3: 1 A JPEG-LS Based Lossless/Lossy Compression Method for Two-Dimensional Electrophoresis Images Source: 2003 International Conference on Informatics, Cybernetics,

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We use Lossless and Near-Lossless compress the important areas and unimportant areas in Two-Dimensional Electrophoresis (2D-Gel) images .

Our system improves traditional JPEG-LS to enhancing the compressed image quality.

Introduction

Page 4: 1 A JPEG-LS Based Lossless/Lossy Compression Method for Two-Dimensional Electrophoresis Images Source: 2003 International Conference on Informatics, Cybernetics,

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Schema (1/2)

Compression flow chart

3

Original 2D-Gel ImageOriginal 2D-Gel Image

Detect Protein’s AreasDetect Protein’s Areas

1

Record Boolean Value of Important Areas

Record Boolean Value of Important Areas

2

JPEG-LS Near-Lossless CompressJPEG-LS Near-Lossless Compress

4

Write Difference Value of Original Image’s Important Areas

Write Difference Value of Original Image’s Important Areas

Difference value Record FileDifference value Record File

Near-Lossless Compressed FileNear-Lossless Compressed File

Page 5: 1 A JPEG-LS Based Lossless/Lossy Compression Method for Two-Dimensional Electrophoresis Images Source: 2003 International Conference on Informatics, Cybernetics,

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Decompression flow chart

Add Difference to Image’s Pixel Value

Add Difference to Image’s Pixel Value

Keep Important Information of 2D-Gel Image

Keep Important Information of 2D-Gel Image

JPEG-LS Near-Lossless Decompress

JPEG-LS Near-Lossless Decompress

Near-Lossless Compressed FileNear-Lossless Compressed File Difference value Record FileDifference value Record File

Near-Lossless Decompressed 2D-Gel Image

Near-Lossless Decompressed 2D-Gel Image

3 4

Schema (2/2)

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Compression Method (1/5)

Original 2D-Gel image

- This is an original 2D-Gel image. X-axis represented the PH value of protein and Y-axis represented the amount molecular weight.

Fig. 1 Original 2D-Gel image X-axis

Y-axis

73 72 75 78 71

71 75 18 4 74

76 5 15 16 10

73 18 23 28 74

75 74 10 73 77

Page 7: 1 A JPEG-LS Based Lossless/Lossy Compression Method for Two-Dimensional Electrophoresis Images Source: 2003 International Conference on Informatics, Cybernetics,

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Compression Method (2/5)

Fetching protein’s area.

- To collect the important protein ‘s areas of 2D-Gel image. The colourful areas will treat as important areas, and white areas will treat as unimportant areas.

Fig. 2 The important part of 2D-Gel image

0 0 0 0 0

0 0 18 4 0

0 5 15 16 10

0 18 23 28 0

0 0 10 0 0

Page 8: 1 A JPEG-LS Based Lossless/Lossy Compression Method for Two-Dimensional Electrophoresis Images Source: 2003 International Conference on Informatics, Cybernetics,

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Compression Method (3/5)

Transform the important parts to Boolean value

- Boolean value True(1) represents important areas, whereas False (0) represents unimportant areas.

Fig. 3 Boolean value record file of important part

0 0 0 0 0

0 0 1 1 0

0 1 1 1 1

0 1 1 1 0

0 0 1 0 0

Page 9: 1 A JPEG-LS Based Lossless/Lossy Compression Method for Two-Dimensional Electrophoresis Images Source: 2003 International Conference on Informatics, Cybernetics,

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Compression Method (4/5)

Image after JPEG-LS Near-Lossless compression

- This is an 2D-Gel image after traditional JPEG-LS Near-Lossless compression.

Fig. 4 Image after JPEG-LS Near-Lossless compression

71 73 77 76 74

72 74 17 6 73

79 3 18 13 8

73 21 23 26 74

74 77 12 75 76

Page 10: 1 A JPEG-LS Based Lossless/Lossy Compression Method for Two-Dimensional Electrophoresis Images Source: 2003 International Conference on Informatics, Cybernetics,

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Compression Method (5/5)

Difference value records important part

- The difference value of 2D-Gel image via the original image and lossless compression will store in a record file.

Fig.5 Difference value record file

0 0 0 0 0

0 0 1 -2 0

0 2 -3 3 2

0 -3 0 2 0

0 0 -2 0 0

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Compression Example

73 72 75 78 71

71 75 18 4 74

76 5 15 16 10

73 18 23 28 74

75 74 10 73 77

Original 2D-Gel Image

71 73 77 76 74

72 74 17 6 73

79 3 18 13 8

73 21 23 26 74

74 77 12 75 76

Image after JPEG-LS Near-Lossless compression

0 0 0 0 0

0 0 1 -2 0

0 2 -3 3 2

0 -3 0 2 0

0 0 -2 0 0

Difference value record file

- =

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Decompression Method (1/3)

Fig 6.Image after JPEG-LS Near-Lossless compression

The image of Near-Lossless decompression

- This is a decompressed image after traditional JPEG-LS Near-Lossless compression.

71 73 77 76 74

72 74 17 6 73

79 3 18 13 8

73 21 23 26 74

74 77 12 75 76

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Decompression Method (2/3)

Modify the protein’s area of important part

- Next, we base on the difference value of pixels for modifying the protein’s area of important parts.

Fig. 7 Difference value record file

0 0 0 0 0

0 0 1 -2 0

0 2 -3 3 2

0 -3 0 2 0

0 0 -2 0 0

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Decompression Method (3/3)

Fig. 8 Our system’s lossless compression of important part.

The lossless image of our system’s important areas

- This complete 2D-Gel image is though traditional JPEG-LS Near-Lossless compression technique.

71 73 77 76 74

72 74 18 4 73

79 5 15 16 10

73 18 23 28 74

74 77 10 75 76

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Decompression Example

Image after JPEG-LS Near-Lossless compression

Difference value record file

Our system’s lossless compression of important part

+ =

71 73 77 76 74

72 74 17 6 73

79 3 18 13 8

73 21 23 26 74

74 77 12 75 76

0 0 0 0 0

0 0 1 -2 0

0 2 -3 3 2

0 -3 0 2 0

0 0 -2 0 0

71 73 77 76 74

72 74 18 4 73

79 5 15 16 10

73 18 23 28 74

74 77 10 75 76

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Results (1/4)

Fig. 9 Partial magnify image of original 2D-Gel image

Fig. 9 is the result of the amplification of dotted frame in Fig. 1.

Page 17: 1 A JPEG-LS Based Lossless/Lossy Compression Method for Two-Dimensional Electrophoresis Images Source: 2003 International Conference on Informatics, Cybernetics,

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LS.

Results (2/4)

Fig.10 is the result of the amplification of dotted frame in Fig. 4.

Page 18: 1 A JPEG-LS Based Lossless/Lossy Compression Method for Two-Dimensional Electrophoresis Images Source: 2003 International Conference on Informatics, Cybernetics,

18 NCHUFig. 11 Partial magnify image of our system.

Results (3/4)

Fig. 11 is the result of the amplification of dotted frame in Fig. 8.

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Result (4/4)Table 1. The Comparison of image quality in traditional JPEG-LS

Near-Lossless with our system (PSNR value). Unit:dB

Results (4/4)

34.57 35.69 36.42 37.47 38.72 40.13 41.85 43.89 46.69 51.08 Our System

33.50 34.56 35.33 36.40 37.65 39.04 40.77 42.82 45.66 50.10 Jpeg-ls2DGel09

34.43 35.27 36.13 37.17 38.31 39.63 41.21 43.16 45.90 50.48 Our System

33.79 34.64 35.50 36.54 37.69 39.01 40.61 42.59 45.35 49.94 Jpeg-ls2DGel08

34.63 35.45 36.35 37.36 38.44 39.77 41.36 43.32 46.03 50.60 Our System

33.83 34.64 35.54 36.57 37.66 39.01 40.61 42.58 45.34 49.93 Jpeg-ls2DGel07

35.70 36.15 37.12 38.14 39.45 40.75 42.39 44.11 46.90 51.34 Our System

34.19 34.77 35.73 36.74 38.03 39.37 41.03 42.78 45.68 50.14 Jpeg-ls2DGel06

34.71 35.48 36.41 37.41 38.57 39.89 41.34 43.36 45.82 51.16 Our System

33.51 34.31 35.24 36.23 37.41 38.75 40.25 42.30 44.85 50.08 Jpeg-ls2DGel05

37.44 32.63 38.48 39.55 40.54 42.00 41.16 41.73 45.58 50.98 Our System

36.11 32.16 37.32 38.37 39.41 40.90 40.47 41.21 44.98 50.29 Jpeg-ls2DGel04

35.81 36.27 36.90 37.59 38.78 40.63 42.31 44.13 46.96 51.29 Our System

34.42 34.97 35.61 36.41 37.57 39.43 41.07 42.93 45.81 50.23 Jpeg-ls2DGel03

35.88 36.75 37.81 38.57 39.80 41.15 42.71 44.70 47.35 51.63 Our System

33.85 34.73 35.74 36.61 37.83 39.20 40.82 42.85 45.60 50.02 Jpeg-ls2DGel02

34.5335.0236.3037.3238.3539.7141.3643.3946.1050.68Our System

33.7534.2335.5336.5537.5738.9740.6342.6745.3750.00Jpeg-ls2DGel01

109876

54321Lossless

lever

34.57 35.69 36.42 37.47 38.72 40.13 41.85 43.89 46.69 51.08 Our System

33.50 34.56 35.33 36.40 37.65 39.04 40.77 42.82 45.66 50.10 Jpeg-ls2DGel09

34.43 35.27 36.13 37.17 38.31 39.63 41.21 43.16 45.90 50.48 Our System

33.79 34.64 35.50 36.54 37.69 39.01 40.61 42.59 45.35 49.94 Jpeg-ls2DGel08

34.63 35.45 36.35 37.36 38.44 39.77 41.36 43.32 46.03 50.60 Our System

33.83 34.64 35.54 36.57 37.66 39.01 40.61 42.58 45.34 49.93 Jpeg-ls2DGel07

35.70 36.15 37.12 38.14 39.45 40.75 42.39 44.11 46.90 51.34 Our System

34.19 34.77 35.73 36.74 38.03 39.37 41.03 42.78 45.68 50.14 Jpeg-ls2DGel06

34.71 35.48 36.41 37.41 38.57 39.89 41.34 43.36 45.82 51.16 Our System

33.51 34.31 35.24 36.23 37.41 38.75 40.25 42.30 44.85 50.08 Jpeg-ls2DGel05

37.44 32.63 38.48 39.55 40.54 42.00 41.16 41.73 45.58 50.98 Our System

36.11 32.16 37.32 38.37 39.41 40.90 40.47 41.21 44.98 50.29 Jpeg-ls2DGel04

35.81 36.27 36.90 37.59 38.78 40.63 42.31 44.13 46.96 51.29 Our System

34.42 34.97 35.61 36.41 37.57 39.43 41.07 42.93 45.81 50.23 Jpeg-ls2DGel03

35.88 36.75 37.81 38.57 39.80 41.15 42.71 44.70 47.35 51.63 Our System

33.85 34.73 35.74 36.61 37.83 39.20 40.82 42.85 45.60 50.02 Jpeg-ls2DGel02

34.5335.0236.3037.3238.3539.7141.3643.3946.1050.68Our System

33.7534.2335.5336.5537.5738.9740.6342.6745.3750.00Jpeg-ls2DGel01

109876

54321Lossless

lever

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Conclusion

We store the unimportant areas by Near-Lossless method. But we store important areas by Lossless method. It is very important to medical images.

Under different lossless level, we can find out our system has better image quality than traditional JPEG-LS.

Therefore, how to compress the size of record file and detect the protein’s location more correctly becoming an important topic in the future.