1 김은정과장님

Dr. Eun Jung KIMNIFDS, KFDA, KOREA

Current trends in researches of intestinal microflora

What is interest in intestinal microflora research?

Introduction of Fundamental Study of Drug Metabolism

Research Group in NIFDS

Current Achievements

Future Prospectives

Research program◦ food safety, food biosecurity, and methods development ◦ antimicrobial resistance in foodborne and commensal bacteria ◦ intestinal microflora and host interactions ◦ environmental biotechnology ◦ microbiological surveillance and diagnostic support of research

Ongoing research projects involved in intestinal microflora◦ evaluation of the mechanisms of inactivation and degradation of third-ge

neration cephalosporins by the bovine intestinal microflora◦ impact of antimicrobial residues on the human gastrointestinal tract Micr

obiota

Unit for Ecology and Physiology of the Digestive Tract (UEPSD)

• Researches on the roles of microflora on food transformation in intestine

• Analysis of microfloral enzymes related to metabolism and digestion of food

• Functional genomic- and metagenomic researches on intestinal microflora

Field of research To elucidate the influence of gastrointestin

al microbiota on human health.

Goal To characterize microorganisms of the

gastrointestinal tract To demonstrate their physiological and

pathophysiological roles

Department of gastro-intestinal microbiology

“The second Human Genome Project”

◦ USA : Human Microbiome Project◦ EU : MetaHIT◦ France : MicroObes◦ Austria : Australian Urogenital Microbiome Consrotium◦ Canada : Canadian Microbiome Initiative◦ China : Meta-GUT◦ Japan : Human Metagenome Contium◦ Singapore : Human Gastric Microbiome◦ International : International Human Microbiome Project

Goal◦ To work under a common set of principles and policies to study and understand

the role of the human microbiome health and causation of disease◦ To use that knowledge to improve the ability to prevent and treat disease

Full Members◦ Canadian Institutes of Health Research (Canada) ◦ European Commission (Europe) ◦ Institut National de la Recherche Agronomique (France) ◦ Japan Science & Technology Agency, JST (Japan) ◦ National Institutes of Health (United States of America) ◦ Commonwealth Scientific and Industrial Research Organisation (Australia) ◦ Medical Research Council (Gambia)

Host Influences in Gut Microbial Ecology

• Age • Diet, Drugs• Environmental • Genetics• Defense mechanism• Stress responses

Intestinal microflora as a new biological resource for future• Only 1 % of intestinal microflora were currently identified, whereas the rest

99% were not yet identified

• Intestinal microflora have great value as resources for biotech industries

To Promote domestic researches on intestinal microflora• Organize a nation-wide research consortium for intenstinal microflora to

promote a international collaboration such as human microbiome

Importance of researches on Korean specific intestinal microflora• Diversity of intestinal microflora- highly depending on age, diet, health and

environment conditions

• Korean has highly different dietary habit and life styles - high expectation for microflora difference

What is interest in intestinal microflora research?

Synopsis of the Research Group

Program : Research group for Fundamental Study of Drug• Total Budget : 2.5 billion won ( ~2 million US $)

• Duration : 3 years (2009~11)

• Principal Investigator of the Program : Professor Dong Hyun KIM

(KyungHee Univ.)

• Program Organizer : Dr. Eun Jung KIM

(Pharmacology Research Division, NIFDS)

• Chairman of Program Advisory Committee : Professor Eungchil CHOI

(Seoul National Univ.)

Program 1Development of toxicological assays using

intestinal microflora

Program Organizer

AdvisioryCommittee

Program 2Researches on metabolic activity of intestinal

microflora

Research Group for Fundamental study of drug metabolism

Program 3Identification and analysis of Korean

specific intestinal microflora

Research project 3-3Inspection of Korean intestinal microflora

diversity according to age, diet and obesity

Research project 1-1Development of metabolite toxicity

testing by using intestinal microfloracultures

Research project 1-2Development of fecalase usable for

evaluation of toxicity induced by intestinal microflora

Research project 1-3Development of in vivo toxicity testing methods using intestinal microflora

Research project 2-1Identification of metaboites and their toxic

metabolism pathways by intestinal microflora

Research project 2-2Study of metabolic activity analysis of drugs by Korea intestinal microflora

Research project 2-3Investigation of database involved in

intestinal microflora

Research project 3-2Deveopment of advanced thechnologyfor quick detection and quantification of

gut microflora

Research project 3-1Standardization for Korean specific

intestinal microflora through isolation and identification of anaerobic bacteria

4/12/2011

Recognized as one of most expected and outstanding future

technologies in 2010 by KISTEP and MEST

Research Publications : Bacteroides faecis sp. nov., isolated

from human faeces and so on.

Research communications : Korean Society of Pharmacology

and so on

International symposium (Oct 2009)

Colloquium between US-FDA and KFDA (Oct 2009)

• March 2010

Public communication by Mass Media - antibiotics and drug absorption

• May 2010

Public communication by Mass Media - Newly identified intestinal microbes from Korean

• Aug 2010

Construction of Korean Intestinal microbes Database

Providing information of Korean intestinal microbes to the public

Development of Metagenomic Chip for identification of intestinal microbes

Establishment of Korean standard fecalase and developing assay methods

Safety evaluation of drug metabolites by intestinal microflora

• Screening of drugs susceptible to intestinal microflora metabolism

Promotion of personalized medicine based on individual intestinal

microflora

• Application of individual or ethnic difference of microflora to find adequate dose of drugs

Future Prospectives