Top Banner
1 2005 Advanced Distributed Learning. All Rights Reserved. Module Module III III
78

1 2005 Advanced Distributed Learning. All Rights Reserved. Module III.

Dec 26, 2015

Download

Documents

Pearl Morrison
Welcome message from author
This document is posted to help you gain knowledge. Please leave a comment to let me know what you think about it! Share it to your friends and learn new things together.
Transcript
Page 1: 1 2005 Advanced Distributed Learning. All Rights Reserved. Module III.

12005 Advanced Distributed Learning. All Rights Reserved.

Module IIIModule III

Page 2: 1 2005 Advanced Distributed Learning. All Rights Reserved. Module III.

22005 Advanced Distributed Learning. All Rights Reserved.

Collection of EvidenceCollection of EvidenceProper Collection, Packaging & Preservation of DNA Evidence

Page 3: 1 2005 Advanced Distributed Learning. All Rights Reserved. Module III.

32005 Advanced Distributed Learning. All Rights Reserved.

Learning Objectives Demonstrate proficiency in at least 2 methods of

collecting biological evidence

Recognize at least 2 improper methods of

packaging biological evidence

Explain the importance of proper storage of

biological evidence

Page 4: 1 2005 Advanced Distributed Learning. All Rights Reserved. Module III.

42005 Advanced Distributed Learning. All Rights Reserved.

Rule of “Thumb” It’s better to collect it and not submit it than

to not have collected it at all!

Keep lines of communication open between you and the laboratory

Page 5: 1 2005 Advanced Distributed Learning. All Rights Reserved. Module III.

52005 Advanced Distributed Learning. All Rights Reserved.

Remember Small items – submit entire item

Large items – submit a portion of the item

Page 6: 1 2005 Advanced Distributed Learning. All Rights Reserved. Module III.

62005 Advanced Distributed Learning. All Rights Reserved.

Amount to Collect• Early DNA - Size of quarter

• Serology - Size of dime

• Current DNA - If you can see it, we can try it

Page 7: 1 2005 Advanced Distributed Learning. All Rights Reserved. Module III.

72005 Advanced Distributed Learning. All Rights Reserved.

Collection of Blood Evaluate the item/stain and its

probative value Swab the stain (works best on

non-absorbent surfaces) Scrape the stain Cut the stain from the item Collect the item

Page 8: 1 2005 Advanced Distributed Learning. All Rights Reserved. Module III.

82005 Advanced Distributed Learning. All Rights Reserved.

Swabbing the Stain Lightly moisten the swab (distilled or

sterile water) Rub swab over the stain, rotating swab Use 2 - 4 more swabs

Page 9: 1 2005 Advanced Distributed Learning. All Rights Reserved. Module III.

92005 Advanced Distributed Learning. All Rights Reserved.

Swabbing the Stain Concentrated!! Better to collect it and not use it rather than to

lose the sample altogether Don’t sacrifice other exams!! (latents!)

Page 10: 1 2005 Advanced Distributed Learning. All Rights Reserved. Module III.

102005 Advanced Distributed Learning. All Rights Reserved.

Collecting the Item Recommended

• When staining pattern is important• Item is difficult to swab• Item requires multiple exams

Make sure the item is dry Package to ensure integrity (glass, sharps,

breakables)

Page 11: 1 2005 Advanced Distributed Learning. All Rights Reserved. Module III.

112005 Advanced Distributed Learning. All Rights Reserved.

Collection of Semen Evaluate the stain and its probative value Swab the stain

• Works best on non-absorbent surfaces

Cut the stain from the item Collect the item

Page 12: 1 2005 Advanced Distributed Learning. All Rights Reserved. Module III.

122005 Advanced Distributed Learning. All Rights Reserved.

Collection of Semen : Condom If the condom appears “filled”

• Secure the condom if possible so the inside doesn’t contaminate the outside

• Small hard container closed with• a paperclip• a twist-tie• an electrical tie

Page 13: 1 2005 Advanced Distributed Learning. All Rights Reserved. Module III.

132005 Advanced Distributed Learning. All Rights Reserved.

Possible Victim Vaginal Fluid

From suspect pubic area or fingers• Lightly moisten the swab (distilled or

sterile)

• Swab the entire area, rotating the swab

• Use no more than 2 swabs

Page 14: 1 2005 Advanced Distributed Learning. All Rights Reserved. Module III.

142005 Advanced Distributed Learning. All Rights Reserved.

Victim Sexual Assault Kit Standardized kit to collect

pertinent biological evidence from the body of the victim

Contact your local laboratory to determine which kit they use

Page 15: 1 2005 Advanced Distributed Learning. All Rights Reserved. Module III.

152005 Advanced Distributed Learning. All Rights Reserved.

Suspect Standard Kit• Standardized kit to collect pertinent

biological evidence from the body of the suspect

• Contact your local laboratory to determine which kit they use

Page 16: 1 2005 Advanced Distributed Learning. All Rights Reserved. Module III.

162005 Advanced Distributed Learning. All Rights Reserved.

Collection of Hairs/Fibers Post-It notes placed into envelope or Ziploc bag

• Straight into Ziploc bag NOT recommended due to static electricity

Gloves or forceps to transfer hair/fiber(s) into a glassine envelope

Vacuum is generally not recommended

Talk to YOUR laboratory!

Page 17: 1 2005 Advanced Distributed Learning. All Rights Reserved. Module III.

172005 Advanced Distributed Learning. All Rights Reserved.

Collection of Saliva From a bitemark:

• Moisten one swab and swab area of mark

• Follow with a dry swab to collect any remaining moisture on skin

• Allow swabs to air dry and package together

From Suspect Pubic Area

• Refer to vaginal fluid instructions

Page 18: 1 2005 Advanced Distributed Learning. All Rights Reserved. Module III.

182005 Advanced Distributed Learning. All Rights Reserved.

Known Saliva (Buccal) Swabs Known standard Swab the inside of the cheek area using 2

swabs Rotating them during collection Allow swabs to air dry (or place in swab box)

and package together Submit dry swabs as control swabs

Page 19: 1 2005 Advanced Distributed Learning. All Rights Reserved. Module III.

192005 Advanced Distributed Learning. All Rights Reserved.

Fingernail Scrapings/Clippings Collect only when indicated Clip nails into clean paper with a clean clipper Separating left and right nails

OR Scrape the underside of each nail onto a clean paper Package the scrapers inside this paper

Page 20: 1 2005 Advanced Distributed Learning. All Rights Reserved. Module III.

202005 Advanced Distributed Learning. All Rights Reserved.

Bone• Co-mingled remains, mass graves, skeletal

remains, exhumations, airplane crashes

• Marrow is where blood is made

• Good to use if no blood is available

• Best to collect rib bone or vertebrae

• Place in jar and freeze

Page 21: 1 2005 Advanced Distributed Learning. All Rights Reserved. Module III.

212005 Advanced Distributed Learning. All Rights Reserved.

Teeth

• DNA is in the dental pulp

• Very resistant to decay

• Obtained from teeth of mummies

• Saved baby teeth can be used as secondary DNA standard in missing person cases

• Collect into pill box

Page 22: 1 2005 Advanced Distributed Learning. All Rights Reserved. Module III.

222005 Advanced Distributed Learning. All Rights Reserved.

Organs/Tissue• Collect if no blood is available

• Collect liver or spleen

• Place in jar and freeze

Page 23: 1 2005 Advanced Distributed Learning. All Rights Reserved. Module III.

232005 Advanced Distributed Learning. All Rights Reserved.

Data Bank Samples

Follow your state or local protocol for collecting samples from arrestees and/or convicted offenders

Page 24: 1 2005 Advanced Distributed Learning. All Rights Reserved. Module III.

242005 Advanced Distributed Learning. All Rights Reserved.

Plug in Activity Practice collecting blood evidence Given a variety of substrates Demonstrate proficiency in swabbing,

lifting, scraping and absorbing blood evidence

Determine advantages and disadvantages for each technique

Page 25: 1 2005 Advanced Distributed Learning. All Rights Reserved. Module III.

252005 Advanced Distributed Learning. All Rights Reserved.

Technique Advantages Disadvantages

Swabbing

Lifting

Scraping

Absorbing

Activity 1Collecting Blood Stains

Page 26: 1 2005 Advanced Distributed Learning. All Rights Reserved. Module III.

262005 Advanced Distributed Learning. All Rights Reserved.

Knowledge into Practice

Look at the following items and determine the best way to handle the object

Page 27: 1 2005 Advanced Distributed Learning. All Rights Reserved. Module III.

272005 Advanced Distributed Learning. All Rights Reserved.

Knowledge into Practice

Page 28: 1 2005 Advanced Distributed Learning. All Rights Reserved. Module III.

282005 Advanced Distributed Learning. All Rights Reserved.

Knowledge into Practice

Page 29: 1 2005 Advanced Distributed Learning. All Rights Reserved. Module III.

292005 Advanced Distributed Learning. All Rights Reserved.

Knowledge into Practice

Page 30: 1 2005 Advanced Distributed Learning. All Rights Reserved. Module III.

302005 Advanced Distributed Learning. All Rights Reserved.

Knowledge into Practice

Page 31: 1 2005 Advanced Distributed Learning. All Rights Reserved. Module III.

312005 Advanced Distributed Learning. All Rights Reserved.

Knowledge into Practice

Page 32: 1 2005 Advanced Distributed Learning. All Rights Reserved. Module III.

322005 Advanced Distributed Learning. All Rights Reserved.

Knowledge into Practice

Page 33: 1 2005 Advanced Distributed Learning. All Rights Reserved. Module III.

332005 Advanced Distributed Learning. All Rights Reserved.

Knowledge into Practice

Page 34: 1 2005 Advanced Distributed Learning. All Rights Reserved. Module III.

342005 Advanced Distributed Learning. All Rights Reserved.

Knowledge into Practice

For the following items, indicate how you would collect the evidence

Page 35: 1 2005 Advanced Distributed Learning. All Rights Reserved. Module III.

352005 Advanced Distributed Learning. All Rights Reserved.

Knowledge into Practice

Page 36: 1 2005 Advanced Distributed Learning. All Rights Reserved. Module III.

362005 Advanced Distributed Learning. All Rights Reserved.

Knowledge into Practice

Page 37: 1 2005 Advanced Distributed Learning. All Rights Reserved. Module III.

372005 Advanced Distributed Learning. All Rights Reserved.

Knowledge into Practice

Page 38: 1 2005 Advanced Distributed Learning. All Rights Reserved. Module III.

382005 Advanced Distributed Learning. All Rights Reserved.

Knowledge into Practice Assuming there is semen on the

following items

Identify the best method to collect the evidence:

• Swab the stain• Cut the stain from the item• Collect the entire item

Page 39: 1 2005 Advanced Distributed Learning. All Rights Reserved. Module III.

392005 Advanced Distributed Learning. All Rights Reserved.

Knowledge into Practice

Carpeting

Mattress

Car seat

Tissues

Diaper

Pants

Linoleum floor

“Sex toy”

Page 40: 1 2005 Advanced Distributed Learning. All Rights Reserved. Module III.

402005 Advanced Distributed Learning. All Rights Reserved.

Packaging Materials Paper bags Plastic bags* Pill boxes Envelopes and note pad Glass jars

Paint cans Cardboard boxes Evidence tape Specialized materials

Page 41: 1 2005 Advanced Distributed Learning. All Rights Reserved. Module III.

412005 Advanced Distributed Learning. All Rights Reserved.

Packaging TechniquesChoice of proper container

• Paper is the packaging material of choice

• Plastic should only be used when there is danger of liquids (biohazard or other) penetrating paper

Page 42: 1 2005 Advanced Distributed Learning. All Rights Reserved. Module III.

422005 Advanced Distributed Learning. All Rights Reserved.

Packaging TechniquesAir-Dried

• Evidence should be allowed to air-dry• The optimal method to preserve DNA

Page 43: 1 2005 Advanced Distributed Learning. All Rights Reserved. Module III.

432005 Advanced Distributed Learning. All Rights Reserved.

Packaging TechniquesTissue and liquid samples

•Keep refrigerated

Page 44: 1 2005 Advanced Distributed Learning. All Rights Reserved. Module III.

442005 Advanced Distributed Learning. All Rights Reserved.

Caution!!Never freeze liquid tubes of blood

Be careful, put glass tubes in plastic bag

Page 45: 1 2005 Advanced Distributed Learning. All Rights Reserved. Module III.

452005 Advanced Distributed Learning. All Rights Reserved.

Packaging Techniques Package items separately when recovered

separately

If items found together, then package together

• Clothing in a pile• Cigarettes in an ashtray

Page 46: 1 2005 Advanced Distributed Learning. All Rights Reserved. Module III.

462005 Advanced Distributed Learning. All Rights Reserved.

Packaging Techniques Don’t allow fluids or stains to touch each

other or unaffected areas

Page 47: 1 2005 Advanced Distributed Learning. All Rights Reserved. Module III.

472005 Advanced Distributed Learning. All Rights Reserved.

Packaging Techniques

Minimize interior movement

Page 48: 1 2005 Advanced Distributed Learning. All Rights Reserved. Module III.

482005 Advanced Distributed Learning. All Rights Reserved.

Unacceptable Plastic Multiple items per

bag Stains still wet “Mummy wrap”

Packaging DNA Evidence

Acceptable Paper One item per bag Stains air-dried Do not overfill

bag

Page 49: 1 2005 Advanced Distributed Learning. All Rights Reserved. Module III.

492005 Advanced Distributed Learning. All Rights Reserved.

Labeling Packages Name/initials of the collector

Each person/entity subsequently having custody

Date of collection or transfer

Agency and case #

Victim or suspect name

Brief description of item

Page 50: 1 2005 Advanced Distributed Learning. All Rights Reserved. Module III.

502005 Advanced Distributed Learning. All Rights Reserved.

Labeling Packages Label container before packaging

Page 51: 1 2005 Advanced Distributed Learning. All Rights Reserved. Module III.

512005 Advanced Distributed Learning. All Rights Reserved.

Labeling Packages Label and identify exterior as to content

Page 52: 1 2005 Advanced Distributed Learning. All Rights Reserved. Module III.

522005 Advanced Distributed Learning. All Rights Reserved.

Labeling Packages Label the item with warnings/hazards or other

helpful information:

• Biohazard

• Sharp

• Fragile

• Unloaded

• Etc.

Page 53: 1 2005 Advanced Distributed Learning. All Rights Reserved. Module III.

532005 Advanced Distributed Learning. All Rights Reserved.

Sealing the Package Seal packages with tape

• Prevents items from falling in• Prevents items from falling out• Provides measure of security – evidence can’t

be accessed unless seal is broken

Initial across seal or under the seal

Page 54: 1 2005 Advanced Distributed Learning. All Rights Reserved. Module III.

542005 Advanced Distributed Learning. All Rights Reserved.

Sealing the Package

Acceptable

Scotch tape Packaging tape

Unacceptable

Masking tape Staples

Page 55: 1 2005 Advanced Distributed Learning. All Rights Reserved. Module III.

552005 Advanced Distributed Learning. All Rights Reserved.

Plug in ActivityGiven 10 items:

Practice identifying the properly

packaged evidence

Practice identifying the improperly

packaged evidence

Page 56: 1 2005 Advanced Distributed Learning. All Rights Reserved. Module III.

562005 Advanced Distributed Learning. All Rights Reserved.

Activity 2Identifying Properly Packaged Evidence

Station PackagesGood Bad (explain)

a.

b.

c.

d.

e.

f.

g.

h.

Page 57: 1 2005 Advanced Distributed Learning. All Rights Reserved. Module III.

572005 Advanced Distributed Learning. All Rights Reserved.

Identify the Packaging Error

Page 58: 1 2005 Advanced Distributed Learning. All Rights Reserved. Module III.

582005 Advanced Distributed Learning. All Rights Reserved.

Identify the Packaging Error

Page 59: 1 2005 Advanced Distributed Learning. All Rights Reserved. Module III.

592005 Advanced Distributed Learning. All Rights Reserved.

Identify the Packaging Error

Page 60: 1 2005 Advanced Distributed Learning. All Rights Reserved. Module III.

602005 Advanced Distributed Learning. All Rights Reserved.

Knowledge Into Practice

Review the following items of evidence

Describe how to best package them for preservation and transportation

Page 61: 1 2005 Advanced Distributed Learning. All Rights Reserved. Module III.

612005 Advanced Distributed Learning. All Rights Reserved.

Knowledge Into Practice

Page 62: 1 2005 Advanced Distributed Learning. All Rights Reserved. Module III.

622005 Advanced Distributed Learning. All Rights Reserved.

Knowledge Into Practice

Page 63: 1 2005 Advanced Distributed Learning. All Rights Reserved. Module III.

632005 Advanced Distributed Learning. All Rights Reserved.

Knowledge Into Practice

Page 64: 1 2005 Advanced Distributed Learning. All Rights Reserved. Module III.

642005 Advanced Distributed Learning. All Rights Reserved.

Knowledge Into Practice

Page 65: 1 2005 Advanced Distributed Learning. All Rights Reserved. Module III.

652005 Advanced Distributed Learning. All Rights Reserved.

Knowledge Into Practice

Page 66: 1 2005 Advanced Distributed Learning. All Rights Reserved. Module III.

662005 Advanced Distributed Learning. All Rights Reserved.

Properly Labeled Evidence

Page 67: 1 2005 Advanced Distributed Learning. All Rights Reserved. Module III.

672005 Advanced Distributed Learning. All Rights Reserved.

Evidence Preservation & Storage

Lack of evidence preservation can render a probative piece of evidence useless!

Page 68: 1 2005 Advanced Distributed Learning. All Rights Reserved. Module III.

682005 Advanced Distributed Learning. All Rights Reserved.

Identify the Storage Error

Page 69: 1 2005 Advanced Distributed Learning. All Rights Reserved. Module III.

692005 Advanced Distributed Learning. All Rights Reserved.

Preserving DNA Evidence

Acceptable Keep cool

Bring to lab as soon as possible

Unacceptable Keep warm

Delay in transport to lab

Page 70: 1 2005 Advanced Distributed Learning. All Rights Reserved. Module III.

702005 Advanced Distributed Learning. All Rights Reserved.

Trunk Storage

Page 71: 1 2005 Advanced Distributed Learning. All Rights Reserved. Module III.

712005 Advanced Distributed Learning. All Rights Reserved.

SummaryDO Air dry before packaging Use paper Use separate bags Use plenty of room Seal with scotch tape Initial with permanent marker Keep item cool

Page 72: 1 2005 Advanced Distributed Learning. All Rights Reserved. Module III.

722005 Advanced Distributed Learning. All Rights Reserved.

SummaryDON’T

Package item still wet

Use plastic

Clump in one bag

Use smallest bag

Staple shut

Initial with ballpoint pen

Keep in trunk in July

Page 73: 1 2005 Advanced Distributed Learning. All Rights Reserved. Module III.

732005 Advanced Distributed Learning. All Rights Reserved.

Now What? Are you familiar with your lab’s DNA testing

policies? Do they…

• Work all types of cases?• Work cases regardless of the submission

of a suspect sample?• Work both subject and non-subject cases?

Contact lab personnel for assistance in evaluating evidence with respect to the lab’s policies and capabilities

Page 74: 1 2005 Advanced Distributed Learning. All Rights Reserved. Module III.

742005 Advanced Distributed Learning. All Rights Reserved.

Time to Submit! Follow your agency’s and forensic lab’s

policies• Meet in person?

• Submit only pre-approved items?

• Special times & locations for submission?

• Specific directions?

• Prioritization?– i.e., if you get results on A, don’t worry about B….

Continue the investigation while waiting for results!

Page 75: 1 2005 Advanced Distributed Learning. All Rights Reserved. Module III.

752005 Advanced Distributed Learning. All Rights Reserved.

Review Learning Objectives

Demonstrate proficiency in at least 2

methods of collecting biological evidence

Recognize at least 2 improper methods of

packaging biological evidence

Explain the importance of proper storage of

biological evidence

Page 76: 1 2005 Advanced Distributed Learning. All Rights Reserved. Module III.

762005 Advanced Distributed Learning. All Rights Reserved.

Questions?

Page 77: 1 2005 Advanced Distributed Learning. All Rights Reserved. Module III.

772005 Advanced Distributed Learning. All Rights Reserved.

15 Minutes !

Break

Page 78: 1 2005 Advanced Distributed Learning. All Rights Reserved. Module III.

782005 Advanced Distributed Learning. All Rights Reserved.

Film Credits

CSI: Las Vegas

Season Four

Alliance Atlantis

Anthony Zuiker-Producer

The Andy Griffith Show

Desilu Productions

For educational purposes onlyFor educational purposes only