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Bradley W. Christian, McLennan Community College© 2016 Pearson Education, Inc.
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Chapter 6: Microbial Growth
Physical Requirements
ChemicalRequirements
Growth of Bacterial Cultures
Measurement of GrowthCulture Media
FINAL ROUND
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Topic 1: Physical Requirements $100 Question
The optimum pH for MOST bacteria is near
a. 1.b. 5.c. 7.d. 9.
ANSWER
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Topic 1: Physical Requirements $100 Answer
The optimum pH for MOST bacteria is near
a. 1.b. 5.c. 7.d. 9.
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Page 4
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Topic 1: Physical Requirements $200 Question
A psychrophile has an optimal growth temperature of about
a. 15°C.b. 25°C.c. 37°C.d. 100°C.
ANSWER
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Topic 1: Physical Requirements $200 Answer
A psychrophile has an optimal growth temperature of about
a. 15°C.b. 25°C.c. 37°C.d. 100°C.
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Topic 1: Physical Requirements $300 Question
Organisms that require high salt concentrations for growth are called
a. thermophiles.b. obligate halophiles.c. acidophiles.d. anaerobes.
ANSWER
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Topic 1: Physical Requirements $300 Answer
Organisms that require high salt concentrations for growth are called
a. thermophiles.b. obligate halophiles.c. acidophiles.d. anaerobes.
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Topic 1: Physical Requirements $400 Question
What is/are the energy source(s) utilized by primary producers in the hydrothermal vents on the ocean floor?
a. lightb. hydrogen sulfidec. organic moleculesd. carbon dioxide
ANSWER
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Topic 1: Physical Requirements $400 Answer
What is/are the energy source(s) utilized by primary producers in the hydrothermal vents on the ocean floor?
a. lightb. hydrogen sulfidec. organic moleculesd. carbon dioxide
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Topic 1: Physical Requirements $500 Question
Addition of salts preserves foods because they
a. lower pH.b. increase osmotic pressure.c. create an isotonic environment.d. lower osmotic pressure.
ANSWER
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Topic 1: Physical Requirements $500 Answer
Addition of salts preserves foods because they
a. lower pH.b. increase osmotic pressure.c. create an isotonic environment.d. lower osmotic pressure.
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Topic 2: Chemical Requirements $100 Question
Chemoautotrophs and photoautotrophs derive their carbon from
a. carbon dioxide.b. proteins.c. lipids.d. carbohydrates.
ANSWER
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Topic 2: Chemical Requirements $100 Answer
Chemoautotrophs and photoautotrophs derive their carbon from
a. carbon dioxide.b. proteins.c. lipids.d. carbohydrates.
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Topic 2: Chemical Requirements $200 Question
Some microorganisms use gaseous nitrogen directly from the atmosphere in a process called
a. denitrification.b. photosynthesis.c. nitrogen fixation.d. nitrogen synthesis.
ANSWER
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Topic 2: Chemical Requirements $200 Answer
Some microorganisms use gaseous nitrogen directly from the atmosphere in a process called
a. denitrification.b. photosynthesis.c. nitrogen fixation.d. nitrogen synthesis.
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Topic 2: Chemical Requirements $300 Question
An organism that uses oxygen when present but can grow without oxygen is called a(n)
a. obligate aerobe.b. facultative anaerobe.c. microaerophile.d. aerotolerant anaerobe.
ANSWER
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Topic 2: Chemical Requirements $300 Answer
An organism that uses oxygen when present but can grow without oxygen is called a(n)
a. obligate aerobe.b. facultative anaerobe.c. microaerophile.d. aerotolerant anaerobe.
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Topic 2: Chemical Requirements $400 Question
Which organism is killed by atmospheric oxygen?
a. facultative anaerobeb. obligate aerobec. obligate anaerobed. aerotolerant anaerobe
ANSWER
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Topic 2: Chemical Requirements $400 Answer
Which organism is killed by atmospheric oxygen?
a. facultative anaerobeb. obligate aerobec. obligate anaerobed. aerotolerant anaerobe
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Topic 2: Chemical Requirements $500 Question
Toxic hydrogen peroxide in some bacteria can be broken down into water and oxygen by the enzyme
a. superoxide dismutase.b. peroxidase.c. hydroxylase.d. catalase.
ANSWER
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Topic 2: Chemical Requirements $500 Answer
Toxic hydrogen peroxide in some bacteria can be broken down into water and oxygen by the enzyme
a. superoxide dismutase.b. peroxidase.c. hydroxylase.d. catalase.
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Topic 3: Growth of Bacterial Cultures $100 Question
Generation time can best be defined as
a. the length of time it takes for lag phase.b. the duration of log phase.c. the time it takes for a cell to divide.d. how many generations a cell can divide.
ANSWER
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Topic 3: Growth of Bacterial Cultures $100 Answer
Generation time can best be defined as
a. the length of time it takes for lag phase.b. the duration of log phase.c. the time it takes for a cell to divide.d. how many generations a cell can divide.
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Topic 3: Growth of Bacterial Cultures $200 Question
Bacterial growth is usually graphed using ________ scales.
a. arithmeticb. exponentialc. logarithmic (semi-logarithmic)d. inverse
ANSWER
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Topic 3: Growth of Bacterial Cultures $200 Answer
Bacterial growth is usually graphed using ________ scales.
a. arithmeticb. exponentialc. logarithmic (semi-logarithmic)d. inverse
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Topic 3: Growth of Bacterial Cultures $300 Question
In which growth phase is there intense activity preparing for population growth, but no increase in population?
a. stationary phaseb. log phasec. death phased. lag phase
ANSWER
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Topic 3: Growth of Bacterial Cultures $300 Answer
In which growth phase is there intense activity preparing for population growth, but no increase in population?
a. stationary phaseb. log phasec. death phased. lag phase
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Topic 3: Growth of Bacterial Cultures $400 Question
Which of the following is NOT a possible reason why exponential growth stops?
a. accumulation of waste productsb. exhaustion of nutrientsc. harmful changes in pHd. bacteria becoming dormant
ANSWER
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Topic 3: Growth of Bacterial Cultures $400 Answer
Which of the following is NOT a possible reason why exponential growth stops?
a. accumulation of waste productsb. exhaustion of nutrientsc. harmful changes in pHd. bacteria becoming dormant
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Topic 3: Growth of Bacterial Cultures $500 Question
In the stationary phase,
a. the number of microbial deaths exceeds the number of new cells formed.
b. the number of microbial deaths is equal to the number of new cells formed.
c. new cells are no longer formed because of adverse environmental conditions.
d. cellular reproduction is most active.ANSWER
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Topic 3: Growth of Bacterial Cultures $500 Answer
In the stationary phase,
a. the number of microbial deaths exceeds the number of new cells formed.
b. the number of microbial deaths is equal to the number of new cells formed.
c. new cells are no longer formed because of adverse environmental conditions.
d. cellular reproduction is most active.
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Topic 4: Culture Media $100 Question
Which type of medium suppresses the growth of unwanted bacteria and encourages growth of desired microbes?
a. differential mediab. complex mediac. selective mediad. enrichment media
ANSWER
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Topic 4: Culture Media $100 Answer
Which type of medium suppresses the growth of unwanted bacteria and encourages growth of desired microbes?
a. differential mediab. complex mediac. selective mediad. enrichment media
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Topic 4: Culture Media $200 Question
Microbes that are introduced into a culture medium to initiate growth are called a(n)
a. inoculum.b. culture.c. specimen.d. medium.
ANSWER
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Topic 4: Culture Media $200 Answer
Microbes that are introduced into a culture medium to initiate growth are called a(n)
a. inoculum.b. culture.c. specimen.d. medium.
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Topic 4: Culture Media $300 Question
What is added to a medium when it is desirable to grow bacteria on a solid medium?
a. glucoseb. magnesium sulfatec. sodium thioglycollated. agar
ANSWER
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Topic 4: Culture Media $300 Answer
What is added to a medium when it is desirable to grow bacteria on a solid medium?
a. glucoseb. magnesium sulfatec. sodium thioglycollated. agar
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Topic 4: Culture Media $400 Question
A culture medium made of extracts from yeasts, meat, or plants is a ________ medium.
a. complexb. selectivec. differentiald. chemically defined
ANSWER
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Topic 4: Culture Media $400 Answer
A culture medium made of extracts from yeasts, meat, or plants is a ________ medium.
a. complexb. selectivec. differentiald. chemically defined
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Topic 4: Culture Media $500 Question
Labs intended for highly infectious airborne pathogens, such as tuberculosis, are classified as
a. BSL-1.b. BSL-2.c. BSL-3.d. BSL-4.
ANSWER
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Topic 4: Culture Media $500 Answer
Labs intended for highly infectious airborne pathogens, such as tuberculosis, are classified as
a. BSL-1.b. BSL-2.c. BSL-3.d. BSL-4.
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Topic 5: Measurement of Growth $100 Question
A spectrophotometer can be used to measure
a. metabolic activity.b. turbidity.c. dry weight.d. pathogenicity.
ANSWER
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Topic 5: Measurement of Growth $100 Answer
A spectrophotometer can be used to measure
a. metabolic activity.b. turbidity.c. dry weight.d. pathogenicity.
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Topic 5: Measurement of Growth $200 Question
The method in which a measured volume of bacterial suspension is placed within a defined area on a microscope slide is called the
a. direct microscope count.b. pour plate method.c. spread plate method.d. most probable number method.
ANSWER
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Topic 5: Measurement of Growth $200 Answer
The method in which a measured volume of bacterial suspension is placed within a defined area on a microscope slide is called the
a. direct microscope count.b. pour plate method.c. spread plate method.d. most probable number method.
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Topic 5: Measurement of Growth $300 Question
Which process is used to ensure that plates contain 30 to 300 colonies when counted?
a. turbidityb. serial dilutionc. filtrationd. pour plates
ANSWER
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Topic 5: Measurement of Growth $300 Answer
Which process is used to ensure that plates contain 30 to 300 colonies when counted?
a. turbidityb. serial dilutionc. filtrationd. pour plates
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Topic 5: Measurement of Growth $400 Question
Which is the best technique to use to measure the growth of filamentous organisms?
a. most probable number methodb. direct microscopic countc. turbidityd. dry weight
ANSWER
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Topic 5: Measurement of Growth $400 Answer
Which is the best technique to use to measure the growth of filamentous organisms?
a. most probable number methodb. direct microscopic countc. turbidityd. dry weight
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Topic 5: Measurement of Growth $500 Question
Which method is a statistical estimating technique used to determine the number of bacteria in a sample?
a. turbidityb. spread plate methodc. most probable number methodd. pour plate method
ANSWER
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Topic 5: Measurement of Growth $500 Answer
Which method is a statistical estimating technique used to determine the number of bacteria in a sample?
a. turbidityb. spread plate methodc. most probable number methodd. pour plate method
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FINAL ROUND Question
Starting with one bacterial cell, how many cells would you have after 10 generations?
a. 10b. 20c. 200d. 1024
ANSWER
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FINAL ROUND Answer
Starting with one bacterial cell, how many cells would you have after 10 generations?
a. 10b. 20c. 200d. 1024
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