· Web viewelongation factor 1-alpha, EF-1, alpha, protein biosynthesis; peptide chain elongation Up regulated opLeV0100009418 SGN-U230999 2.340 5.1 ATP synthase CF0 A chain, PS.lightreaction.ATP
This document is posted to help you gain knowledge. Please leave a comment to let me know what you think about it! Share it to your friends and learn new things together.
Transcript
Suppl Table SI. Metabolite contents of pericarp samples taken from immature green
fruits (22-25 DPA). Normalised to the wild type fold difference values are presented
as mean ± SE of determinations from six independent samples, those determined by
the t-test to be significantly different from wild type are set in bold type
WT antiGSA4 antiGSA8Amino acidsAlanine 1.0
0 0.1
71.39
0.28
0.72
0.25
Asparagine 1.00
0.12
1.16
0.32
0.99
0.37
Aspartate 1.00
0.10
4.64
0.10
2.66
0.17
Beta-alanine 1.00
0.19
1.09
0.17
0.70
0.33
GABA 1.00
0.18
1.08
0.16
0.87
0.33
Glutamate 1.00
0.19
1.14
0.33
0.70
0.28
Glutamine 1.00
0.19
0.82
0.29
0.54
0.30
Glycine 1.00
0.15
1.24
0.21
1.20
0.26
Isoleucine 1.00
0.21
1.40
0.32
1.21
0.40
Leucine 1.00
0.24
1.16
0.29
0.83
0.42
Lysine 1.00
0.19
0.81
0.30
0.83
0.55
Methionine 1.00
0.14
1.26
0.23
1.11
0.34
Phenylalanine 1.00
0.22
1.24
0.22
0.64
0.38
Proline 1.00
0.21
1.11
0.16
0.93
0.27
Serine 1.00
0.09
1.48
0.22
1.32
0.28
Threonine 1.00
0.16
1.03
0.17
1.14
0.38
Tyrosine 1.00
0.14
1.06
0.18
0.88
0.40
Valine 1.00
0.17
1.24
0.23
1.23
0.36
Organic acidsCitrate 1.0
0 0.1
00.90
0.09
1.17
0.16
Dehydroascorbate 1.00
0.06
1.03
0.10
0.95
0.06
Fumarate 1.00
0.10
0.92
0.06
1.45
0.23
Galacturonate 1.00
0.32
1.04
0.14
0.82
0.23
Malate 1.00
0.10
1.59
0.07
1.29
0.18
Succinate 1.00
0.18
0.74
0.05
0.92
0.09
Threonate 1.00
0.10
0.90
0.03
0.97
0.06
1
OthersBenzoate 1.0
0 0.1
01.21
0.12
1.12
0.11
Fructose 1.00
0.02
0.99
0.01
1.01
0.03
Galactinol 1.00
0.12
0.78
0.08
0.67
0.04
Glucose 1.00
0.02
0.99
0.02
1.04
0.02
Glycerol 1.00
0.07
1.03
0.11
1.07
0.09
Hexadecanoate 1.00
0.03
1.06
0.04
1.04
0.02
Inositol 1.00
0.08
1.11
0.05
1.44
0.15
Octadecanoic acid 1.00
0.02
1.03
0.03
1.01
0.02
Phosphoric acid 1.00
0.11
1.05
0.10
1.02
0.16
Putrescine 1.00
0.13
0.96
0.07
1.05
0.12
Quinate, 3-caffeoyl-, trans- 1.00
0.10
0.79
0.18
0.86
0.14
Sucrose 1.00
0.12
0.78
0.17
0.93
0.14
Urea 1.00
0.09
0.77
0.06
0.87
0.14
2
Suppl Table SII. Significantly altered transcripts of pericarp samples taken from immature green fruits (22-25DPA), line aGSA4. Cut-off 3-fold changes.
TOM2 ID SGN log2 Fold changes DescriptionDown regulated
Suppl Table SIII. Significantly altered transcripts of pericarp samples taken from immature green fruits (22-25DPA), line aGSA8. Cut-off 3-fold changes.
TOM ID SGN log2 Fold changes DescriptionDown regulated
opLeV0100003178 SGN-U215328 -5.734 53.2 glutathione S-transferase T3, putativeopLeV0100009763 L39279 -3.546 11.7 glutamate-1-semialdehyde 2,1-aminomutase 1 (GSA 1)opLeV0100003434 SGN-U215474 -3.368 10.3 glutamate-1-semialdehyde 2,1-aminomutase 1 (GSA 1)opLeV0100002789 SGN-U222105 -2.784 6.9 unknownopLeV0100002010 SGN-U226185 -2.657 6.3 unknownopLeV0100006717 SGN-U213668 -2.559 5.9 BURP domain-containing protein, polygalacturonase, cell wall degredation; fruit softeningopLeV0100003541 SGN-U228980 -2.324 5.0 unknownopLeV0100011604 SGN-U212922 -2.296 4.9 pathogenesis-related protein, ethylene-induced, defense responses; senescenceopLeV0100010369 SGN-U219412 -2.201 4.6 thylakoid membrane one helix protein (OHP), PS.lightreaction.photosystem IIopLeV0100000499 SGN-U212696 -1.965 3.9 23.6 kDa mitochondrial small heat shock protein (HSP23.6-M)opLeV0100006726 BT012912 -1.887 3.7 RNA.regulation of transcription.MYB-related transcription factor family, unknownopLeV0100008850 SGN-U215385 -1.876 3.7 peroxidase 12 (PER12) (P12) (PRXR6), cellulose and pectin-containing cell wallopLeV0100004108 SGN-U215329 -1.812 3.5 glutathione S-transferase, putativeopLeV0100000299 SGN-U213431 -1.706 3.3 cytochrome P450 72A1, CYPLXXII, secologanin synthase, monooxygenaseopLeV0100009043 SGN-U217418 -1.687 3.2 heat shock protein 81-1 (HSP81-1)opLeV0100011496 SGN-U213187 -1.599 3.0 pectin methylesterase, putative, cell wall.pectin*esterases.PMEopLeV0100004421 SGN-U219963 -1.513 2.9 DNA repair; DNA-3-methyladenine glycosylase I activityopLeV0100003157 SGN-U212665 -1.447 2.7 kinase, calcium-dependent protein kinase, signal transduction; CDPK; calcium signalingopLeV0100007302 SGN-U213680 -1.405 2.6 unknownopLeV0100005772 SGN-U216469 -1.378 2.6 17.6 kDa class II heat shock protein (HSP17.6-CII)opLeV0100008764 SGN-U226517 -1.376 2.6 Bet v I allergen family protein, molecular_function_unknown; nucleusopLeV0100001707 U72396 -1.326 2.5 stress.abiotic.heat, unknown
Up regulatedopLeV0100008761 SGN-U213016 2.912 7.5 serine-type protease inhibitor, response to wounding or fungusopLeV0100009813 SGN-U213785 2.559 5.9 receptor-like protein kinase 4, putative; serine/threonine receptor kinaseopLeV0100000194 SGN-U218283 2.536 5.8 SKP1 interacting partner 3-related, lectin, phloem-specific lectinopLeV0100000853 X51904 2.275 4.8 unknownopLeV0100004133 SGN-U213338 2.196 4.6 trypsin and protease inhibitor family protein, mitochondrion;endopeptidase inhibitor activityopLeV0100000545 SGN-U213120 2.180 4.5 oxidoreductase, 2OG-Fe(II) oxygenase family protein, unknownopLeV0100003599 SGN-U219846 2.140 4.4 DNAJ heat shock N-terminal domain-containing protein, protein folding
Suppl Table SIV. Wilcoxon rank sum test for transcripts of pericarp samples taken from immature green fruits (22-25DPA), line aGSA4. Bins assignement see MapMan description. Highlighted bins are those with significantly altered transcripts: green – down regulated, red – up regulated, yellow – both up and down.
22.1 polyamine metabolism.synthesis 17 0.0280926.10 misc.cytochrome P450 39 0.0248326.22 misc.short chain dehydrogenase/reductase (SDR) 15 0.04175126.3 misc.gluco-, galacto- and mannosidases 16 0.0286727.3.22 RNA.regulation of transcription.HB,Homeobox transcription factor family 13 0.00256666727.3.23 RNA.regulation of transcription.HSF,Heat-shock transcription factor family 8 0.003157327.3.26 RNA.regulation of transcription.MYB-related transcription factor family 9 0.0378927.3.29 RNA.regulation of transcription.TCP transcription factor family 4 0.039427.3.54 RNA.regulation of transcription.Histone acetyltransferases 5 0.0336727.3.65 RNA.regulation of transcription.Polycomb Group (PcG) 2 0.0353827.3.66 RNA.regulation of transcription.Psudo ARR transcription factor family 2 0.02382229.2.1.1 protein.synthesis.mito/plastid ribosomal protein.plastid 19 2.32E-0729.2.1.99 protein.synthesis.chloroplast/mito - plastid ribosomal protein.unknown 8 0.002997529.2.2 protein.synthesis.misc ribososomal protein 154 3.42E-0629.3.4.99 protein.targeting.secretory pathway.unspecified 22 0.003229.4 protein.postranslational modification 140 0.0063829.5.11.1 protein.degradation.ubiquitin.ubiquitin 18 0.0134429.5.11.20 protein.degradation.ubiquitin.proteasom 26 0.0027729.5.11.4.3.3 protein.degradation.ubiquitin.E3.SCF.cullin 7 0.0243229.5.4 protein.degradation.aspartate protease 7 0.0233329.5.7 protein.degradation.metalloprotease 10 0.01928429.6 protein.folding 24 0.01621729.8 protein assembly and cofactor ligation 7 0.04681230.2.17 signalling.receptor kinases.DUF 26 4 0.02099930.3 signalling.calcium 65 2.50E-0430.5 signalling.G-proteins 67 0.0167531.1 cell.organisation 85 0.0042331.4 cell. vesicle transport 39 0.0125634.1 transport.p- and v-ATPases 10 0.0463834.18 transport.unspecified anions 2 0.0425635.2 not assigned.unknown 954 0.00319854.14 glycolysis.PEPCase 6 0.0044
8
Suppl Table SV. Wilcoxon rank sum test for transcripts of pericarp samples taken from immature green fruits (22-25DPA), line aGSA8. Bins assignement see MapMan description. Highlighted bins are those with significantly altered transcripts: green – down regulated, red – up regulated, yellow – both up and down.
22.1.3 polyamine metabolism.synthesis.arginine decarboxylase 3 0.007623 nucleotide metabolism 47 0.006723.1.3 nucleotide metabolism.synthesis.PRS-PP 4 0.020126.21 misc.protease inhibitor/seed storage/lipid transfer protein (LTP) family protein 7 0.046826.22 misc.short chain dehydrogenase/reductase (SDR) 15 0.026127.1 RNA.processing 66 0.011327.1.20 RNA.processing.degradation dicer 4 0.014927.3.23 RNA.regulation of transcription.HSF,Heat-shock transcription factor family 8 0.021227.3.40 RNA.regulation of transcription.Aux/IAA family 6 0.025428.1 DNA.synthesis/chromatin structure 60 0.002728.1.3 DNA.synthesis/chromatin structure.histone 31 0.000029.1 protein.aa activation 22 0.005829.2.1.1 protein.synthesis.mito/plastid ribosomal protein.plastid 19 0.007929.2.1.99 protein.synthesis.chloroplast/mito - plastid ribosomal protein.unknown 8 0.005129.2.2 protein.synthesis.misc ribososomal protein 154 0.011529.5 protein.degradation 296 0.041729.5.11.1 protein.degradation.ubiquitin.ubiquitin 18 0.051429.5.11.20 protein.degradation.ubiquitin.proteasom 26 0.025129.5.11.4.3.3 protein.degradation.ubiquitin.E3.SCF.cullin 7 0.025329.5.3 protein.degradation.cysteine protease 15 0.04303.2 minor CHO metabolism.trehalose 4 0.051430.10 signalling.phosphorelay 3 0.045530.11 signalling.light, both, more down 30 0.004530.2.24 signalling.receptor kinases.S-locus glycoprotein like 4 0.006830.2.8.2 signalling.receptor kinases.leucine rich repeat VIII-2 3 0.021931.1 cell.organisation 85 0.004634.19.1 transport.Major Intrinsic Proteins.PIP 8 0.036834.8 transport.metabolite transporters at the envelope membrane 5 0.033034.9 transport.metabolite transporters at the mitochondrial membrane 18 0.01404.14 glycolysis.PEPCase 6 0.04308.1.3 TCA / org. transformation.TCA.aconitase 2 0.02848.3 TCA / org. transformation.carbonic anhydrases 3 0.0446
10
Suppl Table SI. Metabolite contents of pericarp samples taken from immature green
fruits (22-25 DPA). Normalised to the wild type fold difference values are presented
as mean ± SE of determinations from six independent samples, those determined by
the t-test to be significantly different from wild type are set in bold type
WT antiGSA4 antiGSA8Amino acidsAlanine 1.0
0 0.1
71.39
0.28
0.72
0.25
Asparagine 1.00
0.12
1.16
0.32
0.99
0.37
Aspartate 1.00
0.10
4.64
0.10
2.66
0.17
Beta-alanine 1.00
0.19
1.09
0.17
0.70
0.33
GABA 1.00
0.18
1.08
0.16
0.87
0.33
Glutamate 1.00
0.19
1.14
0.33
0.70
0.28
Glutamine 1.00
0.19
0.82
0.29
0.54
0.30
Glycine 1.00
0.15
1.24
0.21
1.20
0.26
Isoleucine 1.00
0.21
1.40
0.32
1.21
0.40
Leucine 1.00
0.24
1.16
0.29
0.83
0.42
Lysine 1.00
0.19
0.81
0.30
0.83
0.55
Methionine 1.00
0.14
1.26
0.23
1.11
0.34
Phenylalanine 1.00
0.22
1.24
0.22
0.64
0.38
Proline 1.00
0.21
1.11
0.16
0.93
0.27
Serine 1.00
0.09
1.48
0.22
1.32
0.28
Threonine 1.00
0.16
1.03
0.17
1.14
0.38
Tyrosine 1.00
0.14
1.06
0.18
0.88
0.40
Valine 1.00
0.17
1.24
0.23
1.23
0.36
Organic acidsCitrate 1.0
0 0.1
00.90
0.09
1.17
0.16
Dehydroascorbate 1.00
0.06
1.03
0.10
0.95
0.06
Fumarate 1.00
0.10
0.92
0.06
1.45
0.23
Galacturonate 1.00
0.32
1.04
0.14
0.82
0.23
Malate 1.00
0.10
1.59
0.07
1.29
0.18
Succinate 1.00
0.18
0.74
0.05
0.92
0.09
Threonate 1.0 0.1 0.9 0.0 0.9 0.0
11
0 0 0 3 7 6OthersBenzoate 1.0
0 0.1
01.21
0.12
1.12
0.11
Fructose 1.00
0.02
0.99
0.01
1.01
0.03
Galactinol 1.00
0.12
0.78
0.08
0.67
0.04
Glucose 1.00
0.02
0.99
0.02
1.04
0.02
Glycerol 1.00
0.07
1.03
0.11
1.07
0.09
Hexadecanoate 1.00
0.03
1.06
0.04
1.04
0.02
Inositol 1.00
0.08
1.11
0.05
1.44
0.15
Octadecanoic acid 1.00
0.02
1.03
0.03
1.01
0.02
Phosphoric acid 1.00
0.11
1.05
0.10
1.02
0.16
Putrescine 1.00
0.13
0.96
0.07
1.05
0.12
Quinate, 3-caffeoyl-, trans- 1.00
0.10
0.79
0.18
0.86
0.14
Sucrose 1.00
0.12
0.78
0.17
0.93
0.14
Urea 1.00
0.09
0.77
0.06
0.87
0.14
12
Suppl Table SII. Significantly altered transcripts of pericarp samples taken from immature green fruits (22-25DPA), line aGSA4. Cut-off 3-fold changes.
TOM2 ID SGN log2 Fold changes DescriptionDown regulated
Suppl Table SIII. Significantly altered transcripts of pericarp samples taken from immature green fruits (22-25DPA), line aGSA8. Cut-off 3-fold changes.
TOM ID SGN log2 Fold changes DescriptionDown regulated
opLeV0100003178 SGN-U215328 -5.734 53.2 glutathione S-transferase T3, putativeopLeV0100009763 L39279 -3.546 11.7 glutamate-1-semialdehyde 2,1-aminomutase 1 (GSA 1)opLeV0100003434 SGN-U215474 -3.368 10.3 glutamate-1-semialdehyde 2,1-aminomutase 1 (GSA 1)opLeV0100002789 SGN-U222105 -2.784 6.9 unknownopLeV0100002010 SGN-U226185 -2.657 6.3 unknownopLeV0100006717 SGN-U213668 -2.559 5.9 BURP domain-containing protein, polygalacturonase, cell wall degredation; fruit softeningopLeV0100003541 SGN-U228980 -2.324 5.0 unknownopLeV0100011604 SGN-U212922 -2.296 4.9 pathogenesis-related protein, ethylene-induced, defense responses; senescenceopLeV0100010369 SGN-U219412 -2.201 4.6 thylakoid membrane one helix protein (OHP), PS.lightreaction.photosystem IIopLeV0100000499 SGN-U212696 -1.965 3.9 23.6 kDa mitochondrial small heat shock protein (HSP23.6-M)opLeV0100006726 BT012912 -1.887 3.7 RNA.regulation of transcription.MYB-related transcription factor family, unknownopLeV0100008850 SGN-U215385 -1.876 3.7 peroxidase 12 (PER12) (P12) (PRXR6), cellulose and pectin-containing cell wallopLeV0100004108 SGN-U215329 -1.812 3.5 glutathione S-transferase, putativeopLeV0100000299 SGN-U213431 -1.706 3.3 cytochrome P450 72A1, CYPLXXII, secologanin synthase, monooxygenaseopLeV0100009043 SGN-U217418 -1.687 3.2 heat shock protein 81-1 (HSP81-1)opLeV0100011496 SGN-U213187 -1.599 3.0 pectin methylesterase, putative, cell wall.pectin*esterases.PMEopLeV0100004421 SGN-U219963 -1.513 2.9 DNA repair; DNA-3-methyladenine glycosylase I activityopLeV0100003157 SGN-U212665 -1.447 2.7 kinase, calcium-dependent protein kinase, signal transduction; CDPK; calcium signalingopLeV0100007302 SGN-U213680 -1.405 2.6 unknownopLeV0100005772 SGN-U216469 -1.378 2.6 17.6 kDa class II heat shock protein (HSP17.6-CII)opLeV0100008764 SGN-U226517 -1.376 2.6 Bet v I allergen family protein, molecular_function_unknown; nucleusopLeV0100001707 U72396 -1.326 2.5 stress.abiotic.heat, unknown
Up regulatedopLeV0100008761 SGN-U213016 2.912 7.5 serine-type protease inhibitor, response to wounding or fungusopLeV0100009813 SGN-U213785 2.559 5.9 receptor-like protein kinase 4, putative; serine/threonine receptor kinaseopLeV0100000194 SGN-U218283 2.536 5.8 SKP1 interacting partner 3-related, lectin, phloem-specific lectinopLeV0100000853 X51904 2.275 4.8 unknownopLeV0100004133 SGN-U213338 2.196 4.6 trypsin and protease inhibitor family protein, mitochondrion;endopeptidase inhibitor activityopLeV0100000545 SGN-U213120 2.180 4.5 oxidoreductase, 2OG-Fe(II) oxygenase family protein, unknownopLeV0100003599 SGN-U219846 2.140 4.4 DNAJ heat shock N-terminal domain-containing protein, protein folding
Suppl Table SIV. Wilcoxon rank sum test for transcripts of pericarp samples taken from immature green fruits (22-25DPA), line aGSA4. Bins assignement see MapMan description. Highlighted bins are those with significantly altered transcripts: green – down regulated, red – up regulated, yellow – both up and down.
22.1 polyamine metabolism.synthesis 17 0.0280926.10 misc.cytochrome P450 39 0.0248326.22 misc.short chain dehydrogenase/reductase (SDR) 15 0.04175126.3 misc.gluco-, galacto- and mannosidases 16 0.0286727.3.22 RNA.regulation of transcription.HB,Homeobox transcription factor family 13 0.00256666727.3.23 RNA.regulation of transcription.HSF,Heat-shock transcription factor family 8 0.003157327.3.26 RNA.regulation of transcription.MYB-related transcription factor family 9 0.0378927.3.29 RNA.regulation of transcription.TCP transcription factor family 4 0.039427.3.54 RNA.regulation of transcription.Histone acetyltransferases 5 0.0336727.3.65 RNA.regulation of transcription.Polycomb Group (PcG) 2 0.0353827.3.66 RNA.regulation of transcription.Psudo ARR transcription factor family 2 0.02382229.2.1.1 protein.synthesis.mito/plastid ribosomal protein.plastid 19 2.32E-0729.2.1.99 protein.synthesis.chloroplast/mito - plastid ribosomal protein.unknown 8 0.002997529.2.2 protein.synthesis.misc ribososomal protein 154 3.42E-0629.3.4.99 protein.targeting.secretory pathway.unspecified 22 0.003229.4 protein.postranslational modification 140 0.0063829.5.11.1 protein.degradation.ubiquitin.ubiquitin 18 0.0134429.5.11.20 protein.degradation.ubiquitin.proteasom 26 0.0027729.5.11.4.3.3 protein.degradation.ubiquitin.E3.SCF.cullin 7 0.0243229.5.4 protein.degradation.aspartate protease 7 0.0233329.5.7 protein.degradation.metalloprotease 10 0.01928429.6 protein.folding 24 0.01621729.8 protein assembly and cofactor ligation 7 0.04681230.2.17 signalling.receptor kinases.DUF 26 4 0.02099930.3 signalling.calcium 65 2.50E-0430.5 signalling.G-proteins 67 0.0167531.1 cell.organisation 85 0.0042331.4 cell. vesicle transport 39 0.0125634.1 transport.p- and v-ATPases 10 0.0463834.18 transport.unspecified anions 2 0.0425635.2 not assigned.unknown 954 0.00319854.14 glycolysis.PEPCase 6 0.0044
18
Suppl Table SV. Wilcoxon rank sum test for transcripts of pericarp samples taken from immature green fruits (22-25DPA), line aGSA8. Bins assignement see MapMan description. Highlighted bins are those with significantly altered transcripts: green – down regulated, red – up regulated, yellow – both up and down.