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õöqGA "§÷3øù*$(dR\roæ ùúûwüý0,þ)uÿ !"#ÂVÄ$%01] ij#O¹WjVÜWijjV 653100 23 4567ßß&'ùGA (sI)Ó>*A;5ö÷。[ GH+IîùØ,ùÓ-nâ±Dý5-e¦§ ßß GA (sI)Ó>*Aop.üɤخmW5ö÷。[ YZ})Ó>*AopØ.üË/0 GA (s1Wh21ø30A4a5673×îù² GA (s 20mg/L òZf{>*ÛhAopØ.üË/ 20%¾ÛØ8ÄJɤWÔ5.2%Ø 15.0%,ù² 30mg/L fÛhAopØ.üË/ 25% ~30%¾ÛØ9îJɤWÔ 7.0%Ø 11.9%ßà})Ó>* AÓ:£¤a3ú;£¤<=1W®mWí>?。[ Y@Þ5 GA (sò·U)Ó>*AQËÉØ®mW5> ?A×(sÀ"(sjW×øBÓAmCö÷D)¤456 )Ó>*AQ¤&'ù(s 789:; S572  <=>?@ A  <AB; 0517-6611 2018 08-0046-06 TheEffectsofGibberellinGA ConcentrationontheGrowth YieldandQualityofTobaccoUpperLeaves BULingduo JISigui LIJiangzhouetal YuxiBranchofYunnanTobaccoCompany Yuxi Yunnan653100 Abstract Objective Toresearchtheeffectsofdifferentconcentrationsofgibberellin GA onthegrowthofupperleavesoffluecuredto bacco. Method Fieldtestwascarriedinmountainousanddamlandbyoneyearmultisites.Effectsofdifferentconcentrationsofgibberellin GA ontheupperleavesarea dryweight yieldandchemicalcomponentswerestudied. Result Thegrowthrateofbothaverageleafare asanddryweightoftobaccouppersixleaveswereincreasedandthendeclinedasthesprayingconcentrationofGA increasedfrom 0to 50mg/L meanwhilethegrowthratesdeclinedwiththedropofleafposition.Forthemountainoustobacco thesprayingof20mg/LGA in creasedtheleafareaanddryweightoftobaccouppersixleavesby20%whichresultedin5.21%and14.97%yieldincreaseofXinpingand YimenCounties respectively.Forthedamtobacco thesprayingof30mg/LGA increasedleafareasanddryweightby25% -30%which resultedin7.00% and11.91% yieldincreaseofXinpingandEshanCounties respectively.Atthesametime thetwotreatmentscouldre ducethecontentsofnicotine increasethetotalpotassium andimprovethecoordinationofchemicalcomponentsoftobaccoupperleavesin boththemountainousanddam land.Conclusion ThesprayingofappropriateGA concentrationeffectivelypromotedtheexpansion in creasedtheyield andresultedinthecoordinationofchemicalcomponentsinthetobaccoupperleaves.LowerGA concentrationsshowedno obviouseffects whilethehigherGA concentrationsdelayedthematureofupperleavesandaffectedthebakingqualityoftobacco. Keywords Fluecuredtobacco Upperleaves Leafexpansion Quality Gibberellin GA concentration CDEF 3Ä¥Ó%û-¼ª4EF./SW[GH I+í”( 2015YN13 )。 GHIJ ùúû1982 —), ;»,¨<IJ·«â01»¼ ½N4RS®Go¾¿ÀÁ«â01»¼½Ó`ÂØ;4ûµKLMN 2017-12-11 :;MN 2017-12-22 XOOȧO*%{PO*HO 1/3 O*P ¹°O 30% ~45%Y XO P 5 °\¹°b?TUª 1-2 KNgO²HIȧ*IG '.Â" Oäü 3-4 IßzàÈa-ȧ*p` x 5-8 W¿h'?d½XOȧ*¨O* °\p`x<gO¹%#nOØäüd°+ ,-IS§ìH2GA p0sȧ*S,2¤ ÓN?d½È§*5Ä 9-10 ȧO*°N ±º²ìH2§`O~Åpàî± 9-13 XOÈ §*°YìH2pà½79aO1Ä+,j V<ij#ÆuXOOTU¹²: 30 &CØz: XO K326 $O:OO50G§ÂO²HIXOȧ O*\Á^9*ÁDZ\Or°¨a~äü<a >ºuO*°bOTUóWVw>º ë ~;<+,-±ìH2pàYXOȧ*5 Ä\vO¼½XI-IS5Ä ë¼YXOȧ*5 ÄyO¼½9a?d½XOȧ*¨È§ *°×?dãL1 OPQRS 1.1 áâôßà Àw 2016 &IjVܤ2G¨ }YȳZº\8ºF g±²IjV Ü[82J\¥Ú]ÈZº)、 º@2£/¨^kÈ8 ºÃ¤À{{ë'jVO²Ýª {°§oa+ 1 2016 súûáâüÞ÷3$^þij Table1 Themainmeteorologicaldataduringthetobaccogrowingperiod MaytoAugust oftestingareasinYuxiCountyin2016 À2² TestedCounty ¤'$( Averagetemperature ¯ May ¯ June ¯ July ¯ August +,° Rainfall mm ¯ May ¯ June ¯ July ¯ August ÕϲH Sunshinehour ¯ May ¯ June ¯ July ¯ August ¤2 XinpingCounty 21.5 22.3 21.9 21.8 66.3 155.0 31.8 197.9 212.0 160.0 135.0 158.0 º@2 YimenCounty 20.7 22.0 21.5 21.6 80.3 192.3 71.6 163.2 182.0 100.0 75.0 87.0 [82 EshanCounty 20.4 21.7 21.4 21.2 63.4 116.1 93. 126.7 203.0 151.0 108.0 120.0 1.2 áâOP XOvzj V Ü D XO v z K326 À`IH2 4%í¼Õ> 5g/ Ñ200mg ), éÜ#;|Ü/GC_À°¿O ¹`¡^°12∶6∶24 ), °50%)。 1.3 áâË< À§íYÏ0mg/L CK ), þ TUVWXYJ.AnhuiAgric.Sci.2018 46 ): 46-51 99
7

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Page 1: øœß wüý 0 þ uÿ !# V $ % 01 ij#O W VÜW Vahnykx.com/upload/2018-3/2018031457272429.pdf · ıöq(GA3)"´§÷3łø*$(dR\„roæ øœß,wüý,0,þ,)uÿ,!"#,´V˜,$

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 0517-6611(2018)08-0046-06

TheEffectsofGibberellin(GA3)ConcentrationontheGrowth,YieldandQualityofTobaccoUpperLeavesBULingduo,JISigui,LIJiangzhouetal (YuxiBranchofYunnanTobaccoCompany,Yuxi,Yunnan653100)Abstract [Objective]Toresearchtheeffectsofdifferentconcentrationsofgibberellin(GA3)onthegrowthofupperleavesoffluecuredtobacco.[Method]Fieldtestwascarriedinmountainousanddamlandbyoneyearmultisites.Effectsofdifferentconcentrationsofgibberellin(GA3)ontheupperleavesarea,dryweight,yieldandchemicalcomponentswerestudied.[Result]ThegrowthrateofbothaverageleafareasanddryweightoftobaccouppersixleaveswereincreasedandthendeclinedasthesprayingconcentrationofGA3increasedfrom0to50mg/L,meanwhilethegrowthratesdeclinedwiththedropofleafposition.Forthemountainoustobacco,thesprayingof20mg/LGA3increasedtheleafareaanddryweightoftobaccouppersixleavesby20%,whichresultedin5.21% and14.97% yieldincreaseofXinpingandYimenCounties,respectively.Forthedamtobacco,thesprayingof30mg/LGA3increasedleafareasanddryweightby25%-30%,whichresultedin7.00% and11.91% yieldincreaseofXinpingandEshanCounties,respectively.Atthesametime,thetwotreatmentscouldreducethecontentsofnicotine,increasethetotalpotassium,andimprovethecoordinationofchemicalcomponentsoftobaccoupperleavesinboththemountainousanddamland.[Conclusion]ThesprayingofappropriateGA3concentrationeffectivelypromotedtheexpansion,increasedtheyield,andresultedinthecoordinationofchemicalcomponentsinthetobaccoupperleaves.LowerGA3concentrationsshowednoobviouseffects,whilethehigherGA3concentrationsdelayedthematureofupperleavesandaffectedthebakingqualityoftobacco.Keywords Fluecuredtobacco;Upperleaves;Leafexpansion;Quality;Gibberellin(GA3)concentration

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Table1 Themainmeteorologicaldataduringthetobaccogrowingperiod(MaytoAugust)oftestingareasinYuxiCountyin2016

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TestedCounty

¤'$(

Averagetemperature∥℃5̄May

6̄June

7̄July

8̄August

+,°

Rainfall∥mm5̄May

6̄June

7̄July

8̄August

ÕϲH

Sunshinehour∥h5̄May

6̄June

7̄July

8̄August

�¤2

XinpingCounty 21.5 22.3 21.9 21.8 66.3 155.0 131.8 197.9 212.0 160.0 135.0 158.0º@2

YimenCounty 20.7 22.0 21.5 21.6 80.3 192.3 71.6 163.2 182.0 100.0 75.0 87.0[82

EshanCounty 20.4 21.7 21.4 21.2 63.4 116.1 93.0 126.7 203.0 151.0 108.0 120.0

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,J.AnhuiAgric.Sci.2018,46(8):46-51,99

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Fig.3 EffectsofgibberellinconcentrationontheexpansionoftobaccoupperleavesinmountainousanddamlandsofXinpingCounty

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County)

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2 õöq"§y~ü!ôx"ô÷3øù*%&/aroæ

Table2 EffectsofgibberellinconcentrationontheeconomiccharactersoftobaccoupperleavesinthemountainousanddamlandsofXinpingCoun

ty

Tobaccoland

ìH2pà

GA3concentrationmg/L

ÈaOyâ

Proportionoffirstclasstobacco∥%

oaOyâ

Proportionofmiddleclasstobacco∥%

¹°

Yieldkg/hm2

¹g

OutputvalueÎ

/hm2

'f

AveragepriceÎ

/kg8º

Mountainousland 0 30.3 31.5 951.0 19726.5 20.710 31.2 30.6 963.0 20352.0 21.120 32.5 33.0 1000.5 21145.5 21.130 30.2 31.8 972.0 19933.5 20.540 28.6 30.9 945.0 19296.0 20.450 28.3 29.1 912.0 17419.5 19.1

Damland 0 34.2 33.8 985.5 20988.0 21.310 35.5 32.9 1002.0 21841.5 21.820 36.7 33.7 1029.0 22668.0 22.030 37.3 32.7 1054.5 23376.0 22.240 33.7 34.9 1009.5 21490.5 21.350 32.7 31.4 940.5 19878.0 21.1

+

3 õöq"§y~ü!ôx"ô÷3øù*�3%&/a)*roæ

Table3 Effectsofgibberellinconcentrationontheincreaserateofmajoreconomiccharactersoftobaccoupperleavesinthemountainousanddam

landsofXinpingCounty

ìH2pà

GA3concentrationmg/L

Mountainousland∥%

ÈaOyâ

Proportionoffirstclasstobacco

¹°

Yield¹g

Outputvalue

Damland∥%

ÈaOyâ

Proportionoffirstclasstobacco

¹°

Yield¹g

Outputvalue

10 0.87b 1.26b 3.17b 1.23b 1.67b 4.07c20 2.25a 5.21a 7.19a 2.48ab 4.41ab 8.00b30 -0.03c 2.21b 1.05b 3.02a 7.00a 11.38a40 -1.71d -0.63c -2.18c -0.52bc 2.44b 2.39c50 -1.99d -4.10d -11.69d -1.55c -4.57c -5.29d Q

:±R�±Jefg�59v67

(P<0.05) Note:Differentlowercasesinthesamecolumnindicatedsignificantdifferences(P<0.05)

2.2.2 [82Zº\º@28º

。[82Zº\º@28

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(�

4),�2

2ëO¥�ÀK

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20mg/L.

kM¨

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20mg/L.kMd

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,̈° Â N �

94.5kg/hm2、103.5kg/hm2\

3643.5Î

/hm2,̈ñÂN�

5.15%、14.94%\

18.60%;Z

º¨ ° � ì H 2 p à

30mg/L. k M d

, N �

139.0kg/hm2、139.5kg/hm2\

5892.0Î

/hm2,̈ñÂN�

3.69%、11.91%\

17.66%。

9446Ä

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+

4 õöq"§'Eü!ôx(!ü"ôáâ÷3øù*�3%&/aroæ

Table4 Effectsofgibberellinconcentrationontheeconomiccharactersoftobaccoupperleavesinthemountainous(Yimen)anddam(Eshan)lands

Tobaccoland

ìH2pà

GA3concentrationmg/L

ÈaOyâ

Proportionoffirstclasstobacco∥%

oaOyâ

Proportionofmiddleclasstobacco∥%

¹°

Yieldkg/hm2

¹g

OutputvalueÎ

/hm2

'f

AveragepriceÎ

/kg

(º@

) 0 51.3 39.9 691.5 19585.5 28.3Mountainousland 10 53.2 38.8 760.5 21798.0 28.7

20 56.4 39.2 795.0 23229.0 29.230 52.7 36.2 754.5 21159.0 28.040 50.5 37.2 709.5 19207.5 27.150 48.1 35.8 679.5 17689.5 26.0

([8

) 0 57.7 27.4 1171.5 33357.0 28.5Damland 10 58.4 28.9 1207.5 35139.0 29.1

20 60.2 29.7 1242.0 36453.0 29.430 61.4 33.6 1311.0 39249.0 29.940 57.3 30.9 1279.5 37044.0 29.050 55.1 29.4 1218.0 34221.0 28.1

+

5 õöq"§'Eü!ôx(!ü"ô÷3�3%&/a)*roæ

Table5 Effectsofgibberellinconcentrationontheincreaserateofmajoreconomiccharactersoftobaccoupperleavesinthemountainous(Yimen)

anddam(Eshan)lands

ìH2pà

GA3concentrationmg/L

(º@

)Mountainousland∥%

ÈaOyâ

Proportionoffirstclasstobacco

¹°

Yield¹g

Outputvalue

([8

)Damland∥%

ÈaOyâ

Proportionoffirstclasstobacco

¹°

Yield¹g

Outputvalue

10 1.98b 9.98b 11.30b 0.64b 3.07c 5.34c20 5.15a 14.97a 18.60a 2.39ab 6.02b 9.28bc30 1.41b 9.11b 8.03b 3.69a 11.91a 17.66a40 -0.74c 2.60c -1.93c 0.58b 9.22ab 11.05b50 -3.15d -1.74d -9.68d -2.70c 3.97c 2.59c Q

:±R�±Jefg�59v67

(P<0.05) Note:Differentlowercasesinthesamecolumnindicatedsignificantdifferences(P<0.05)

2.3 |}õöq"§÷3øù*åY�9roæ

2.3.1 �¤2\Zº

。�¤2

2ëO¥XOȧ*�IG

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、O�'�+V¨

,P8

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�V+

,Ns�yz·N+

。8ºXO�ìH2pà

20mg/L.kOȧ*P8

、P�r°M¨

,Çs9

、O�r

°M¢

,8�yMÖ:w

1;�ìH2pà

10mg/L.ks�

yMÖ:w

10。ZºXO�ìH2pà

30mg/L.kȧ

*Çs9

、O�r°M¢

,P�r°M¨

,8�yMÖ:w

1,�ìH2pà

10mg/L.ks�yMÖ:w

10,NP8r°

�ìH2pà

20mg/L.kM¨

+

6 õöq"§y~ü!ôx"ô÷3øù*åY�9roæ

Table6 EffectsofgibberellinconcentrationonthechemicalcomponentsoftobaccoupperleavesinmountainousanddamlandsinXinpingCounty

Tobaccoland

ìH2pà

GA3concentrationmg/L

Ps

Totalsugar%

�¸s

Reducingsugar∥%

Çs9

Differencebetweentotalsugarandreducingsugar∥%

P8

Totalnitrogen%

O�

Nicotine%

Potassium%

s

/�

Sugar/Nicotine

8

/�

Nitrogen/Nicotine

0 32.62 27.01 5.61 1.99 3.09 1.83 10.55 0.64Mountainousland 10 28.90 23.92 4.98 2.32 2.93 1.89 9.86 0.79

20 24.85 23.19 1.66 2.35 2.61 2.23 9.51 0.9030 25.61 23.35 2.26 2.30 2.94 2.17 8.71 0.7840 26.18 23.60 2.58 2.00 3.03 2.00 8.63 0.6650 27.41 23.82 3.59 2.01 3.41 1.99 8.03 0.59

0 34.51 27.75 6.76 1.97 2.99 1.84 11.53 0.66Damland 10 28.22 24.43 3.79 2.12 2.81 2.05 10.04 0.75

20 26.53 23.65 2.88 2.42 2.73 2.14 9.71 0.8930 23.37 21.98 1.39 2.23 2.44 2.35 9.57 0.9340 24.33 22.26 2.07 2.20 2.61 2.32 9.33 0.8450 25.99 23.09 2.90 2.09 2.82 2.24 9.22 0.74

05           Â묭®

                         2018s

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7 õöq"§'Eü!ôx(!ü"ô÷3øù*åY�9roæ

Table7 Effectsofgibberellinconcentrationonthechemicalcomponentsoftobaccoupperleavesinmountainous(Yimen)anddam(Eshan)lands

Tobaccoland

ìH2pà

GA3concentrationsmg/L

Ps

Totalsugar%

�¸s

Reducingsugar∥%

Çs9

Differencebetweentotalsugarandreducingsugar∥%

P8

Totalnitrogen%

O�

Nicotine%

Potassium%

s

/�

Sugar/Nicotine

8

/�

Nitrogen/Nicotine

(º@

) 0 28.65 24.83 3.82 2.14 3.44 2.02 8.33 0.62Mountainousland 10 26.12 23.48 2.64 2.38 3.21 2.13 8.14 0.74

20 24.35 21.90 1.45 2.46 3.03 2.32 8.04 0.8130 24.51 22.31 2.40 2.34 3.12 2.28 7.86 0.7540 24.83 21.81 3.42 2.31 3.19 2.25 7.78 0.7250 25.03 22.40 3.63 2.26 3.24 2.11 7.73 0.70

([8

) 0 30.34 25.21 5.13 2.05 3.31 2.31 9.17 0.62Damland 10 26.70 23.70 3.00 2.22 3.06 2.44 8.73 0.73

20 23.89 21.02 2.87 2.39 2.83 2.92 8.44 0.8430 22.53 20.57 1.96 2.41 2.88 2.67 7.82 0.8440 22.87 20.18 2.68 2.34 3.06 2.57 7.47 0.7650 23.42 21.20 3.72 2.28 3.19 2.40 7.34 0.71

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[5]ZHANGLJ,HUANGGL,CAIQX,etal.OptimizetheinteractionsatS4withefficientinhibitorstargeting3Cproteinasefromenterovirus71[J].JMolRecognit,2016,29(11):520-527.

[6]ANASTASINAM,DOMANSKAA,PALMK,etal.Humanpicornavirusesassociatedwithneurologicaldiseasesandtheirneutralizationbyantibodies[J].JGenVirol,2017,98(6):1145-1158.

[7]CHANYK,GACKMU.ViralevasionofintracellularDNAandRNAsensing[J].NatRevMicrobiol,2016,14(6):360-373.

[8]BARRALPM,SARKARD,FISHERPB,etal.RIGIiscleavedduringpicornavirusinfection[J].Virology,2009,391(2):171-176.

[9]YANGY,LIANGYQ,QUL,etal.Disruptionofinnateimmunityduetomitochondrialtargetingofapicornaviralproteaseprecursor[J].ProcNatlAcadSciUSA,2007,104(17):7253-7258.

[10]DRAHOSJ,RACANIELLOVR.CleavageofIPS1incellsinfectedwithhumanrhinovirus[J].JVirol,2009,83(22):11581-11587.

[11]MUKHERJEEA,MOROSKYSA,DELORMEAXFORDE,etal.ThecoxsackievirusB3CproproteasecleavesMAVSandTRIFtoattenuatehosttypeIinterferonandapoptoticsignaling[J].PLoSPathog,2011,7(3):1-14.

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