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The insects will tell you everything, The insects will tell you everything, people lie, but insects don't lie.” people lie, but insects don't lie.” Murray Marks ---forensic anthropologist, Murray Marks ---forensic anthropologist, University of Tennessee University of Tennessee
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“ The insects will tell you everything, people lie, but insects don't lie.” Murray Marks ---forensic anthropologist, University of Tennessee.

Dec 14, 2015

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Page 1: “ The insects will tell you everything, people lie, but insects don't lie.” Murray Marks ---forensic anthropologist, University of Tennessee.

“ “ The insects will tell you The insects will tell you everything, people lie, but insects everything, people lie, but insects

don't lie.”don't lie.”

Murray Marks ---forensic anthropologist, Murray Marks ---forensic anthropologist, University of TennesseeUniversity of Tennessee

Page 2: “ The insects will tell you everything, people lie, but insects don't lie.” Murray Marks ---forensic anthropologist, University of Tennessee.
Page 3: “ The insects will tell you everything, people lie, but insects don't lie.” Murray Marks ---forensic anthropologist, University of Tennessee.

Blowfly Life CycleBlowfly Life Cycle

Page 4: “ The insects will tell you everything, people lie, but insects don't lie.” Murray Marks ---forensic anthropologist, University of Tennessee.
Page 5: “ The insects will tell you everything, people lie, but insects don't lie.” Murray Marks ---forensic anthropologist, University of Tennessee.

The Role of Arthropods (Phylum The Role of Arthropods (Phylum Arthropoda) in Animal DecompositionArthropoda) in Animal Decomposition

Page 6: “ The insects will tell you everything, people lie, but insects don't lie.” Murray Marks ---forensic anthropologist, University of Tennessee.

The Role of Arthropods (Phylum The Role of Arthropods (Phylum Arthropoda) in Animal DecompositionArthropoda) in Animal Decomposition

Key Points:Key Points:

Page 7: “ The insects will tell you everything, people lie, but insects don't lie.” Murray Marks ---forensic anthropologist, University of Tennessee.

The Role of Arthropods (Phylum The Role of Arthropods (Phylum Arthropoda) in Animal DecompositionArthropoda) in Animal Decomposition

Key Points:Key Points:

• Following death, blow flies (Calliphoridae) and flesh flies Following death, blow flies (Calliphoridae) and flesh flies (Sarchophagidae) are the first animals to locate and colonize the (Sarchophagidae) are the first animals to locate and colonize the carcass – arriving within ten minutes of death.carcass – arriving within ten minutes of death.

Page 8: “ The insects will tell you everything, people lie, but insects don't lie.” Murray Marks ---forensic anthropologist, University of Tennessee.
Page 9: “ The insects will tell you everything, people lie, but insects don't lie.” Murray Marks ---forensic anthropologist, University of Tennessee.
Page 10: “ The insects will tell you everything, people lie, but insects don't lie.” Murray Marks ---forensic anthropologist, University of Tennessee.
Page 11: “ The insects will tell you everything, people lie, but insects don't lie.” Murray Marks ---forensic anthropologist, University of Tennessee.
Page 12: “ The insects will tell you everything, people lie, but insects don't lie.” Murray Marks ---forensic anthropologist, University of Tennessee.

(Order: DIPTERA, Family: CALLIPHORIDAE)(Order: DIPTERA, Family: CALLIPHORIDAE)

Green Bottle FlyGreen Bottle Fly

Phaenicia sericataPhaenicia sericata, and , and Phaenicia cuprinaPhaenicia cuprina

Page 13: “ The insects will tell you everything, people lie, but insects don't lie.” Murray Marks ---forensic anthropologist, University of Tennessee.

(Order: DIPTERA, Family: CALLIPHORIDAE)(Order: DIPTERA, Family: CALLIPHORIDAE)

Hairy Maggot Blow FlyHairy Maggot Blow Fly

(Chrysomya rufifacies(Chrysomya rufifacies))

Page 14: “ The insects will tell you everything, people lie, but insects don't lie.” Murray Marks ---forensic anthropologist, University of Tennessee.

(Order: DIPTERA, Family: CALLIPHORIDAE)(Order: DIPTERA, Family: CALLIPHORIDAE)

Oriental Latrine FlyOriental Latrine Fly

Chrysomya megacephalaChrysomya megacephala

Page 15: “ The insects will tell you everything, people lie, but insects don't lie.” Murray Marks ---forensic anthropologist, University of Tennessee.

(Order: DIPTERA, Family: CALLIPHORIDAE)(Order: DIPTERA, Family: CALLIPHORIDAE)

Blue Bottle FlyBlue Bottle Fly

((Calliphora vomitoriaCalliphora vomitoria))

Page 16: “ The insects will tell you everything, people lie, but insects don't lie.” Murray Marks ---forensic anthropologist, University of Tennessee.

(Order: DIPTERA, Family: CALLIPHORIDAE)(Order: DIPTERA, Family: CALLIPHORIDAE)

Black Blow FlyBlack Blow Fly

Phormia reginaPhormia regina

Page 17: “ The insects will tell you everything, people lie, but insects don't lie.” Murray Marks ---forensic anthropologist, University of Tennessee.

(Order: Diptera, Family: Sarcophagidae)(Order: Diptera, Family: Sarcophagidae)

Flesh FliesFlesh Flies

SarcophagaSarcophaga spp. spp.

Page 18: “ The insects will tell you everything, people lie, but insects don't lie.” Murray Marks ---forensic anthropologist, University of Tennessee.

(Order: Diptera, Family: Muscidae)(Order: Diptera, Family: Muscidae)

House flyHouse fly

(Musca domestica(Musca domestica))

Page 19: “ The insects will tell you everything, people lie, but insects don't lie.” Murray Marks ---forensic anthropologist, University of Tennessee.

(Order: Diptera: Family: Calliphoridae)(Order: Diptera: Family: Calliphoridae)

Secondary Screwworm FlySecondary Screwworm Fly

(Cochliomyia macellaria(Cochliomyia macellaria))

Page 20: “ The insects will tell you everything, people lie, but insects don't lie.” Murray Marks ---forensic anthropologist, University of Tennessee.

The Role of Arthropods (Phylum The Role of Arthropods (Phylum Arthropoda) in Animal DecompositionArthropoda) in Animal Decomposition

Key Points:Key Points:

Page 21: “ The insects will tell you everything, people lie, but insects don't lie.” Murray Marks ---forensic anthropologist, University of Tennessee.

The Role of Arthropods (Phylum The Role of Arthropods (Phylum Arthropoda) in Animal DecompositionArthropoda) in Animal Decomposition

Key Points:Key Points:

• Following death, blow flies (Calliphoridae) and flesh flies Following death, blow flies (Calliphoridae) and flesh flies (Sarchophagidae) are the first animals to locate and colonize the (Sarchophagidae) are the first animals to locate and colonize the carcass – arriving within ten minutes of death.carcass – arriving within ten minutes of death.

• The decay process is highly predictable with respect to: 1) the The decay process is highly predictable with respect to: 1) the associated external insect fauna and 2) the internal bacterial associated external insect fauna and 2) the internal bacterial fauna – passing through various stages of ecological succession fauna – passing through various stages of ecological succession from the “fresh” stage to the “skeletal” phase. from the “fresh” stage to the “skeletal” phase.

Page 22: “ The insects will tell you everything, people lie, but insects don't lie.” Murray Marks ---forensic anthropologist, University of Tennessee.

The Role of Arthropods (Phylum The Role of Arthropods (Phylum Arthropoda) in Animal DecompositionArthropoda) in Animal Decomposition

Key Points:Key Points:

• Following death, blow flies (Calliphoridae) and flesh flies Following death, blow flies (Calliphoridae) and flesh flies (Sarchophagidae) are the first animals to locate and colonize the (Sarchophagidae) are the first animals to locate and colonize the carcass – arriving within ten minutes of death.carcass – arriving within ten minutes of death.

• The decay process is highly predictable with respect to: 1) the The decay process is highly predictable with respect to: 1) the associated external insect fauna and 2) the internal bacterial associated external insect fauna and 2) the internal bacterial fauna – passing through various stages of ecological succession fauna – passing through various stages of ecological succession from the “fresh” stage to the “skeletal” phase. from the “fresh” stage to the “skeletal” phase.

• see http://www.deathonline.net/decomposition/index.htmsee http://www.deathonline.net/decomposition/index.htm

Page 23: “ The insects will tell you everything, people lie, but insects don't lie.” Murray Marks ---forensic anthropologist, University of Tennessee.

The Role of Arthropods (Phylum The Role of Arthropods (Phylum Arthropoda) in Animal DecompositionArthropoda) in Animal Decomposition

Key Points:Key Points:

• Following death, blow flies (Calliphoridae) and flesh flies Following death, blow flies (Calliphoridae) and flesh flies (Sarchophagidae) are the first animals to locate and colonize the (Sarchophagidae) are the first animals to locate and colonize the carcass – arriving within ten minutes of death.carcass – arriving within ten minutes of death.

• The decay process is highly predictable with respect to: 1) the The decay process is highly predictable with respect to: 1) the associated external insect fauna and 2) the internal bacterial associated external insect fauna and 2) the internal bacterial fauna – passing through various stages of ecological succession fauna – passing through various stages of ecological succession from the “fresh” stage to the “skeletal” phase. from the “fresh” stage to the “skeletal” phase.

• By studying these stages of decay and the abiotic conditions at By studying these stages of decay and the abiotic conditions at the site (temperature, habitat conditions, etc…), forensic the site (temperature, habitat conditions, etc…), forensic scientists can accurately predict the time of death or post-scientists can accurately predict the time of death or post-mortem interval (PMI). mortem interval (PMI).

Page 24: “ The insects will tell you everything, people lie, but insects don't lie.” Murray Marks ---forensic anthropologist, University of Tennessee.
Page 25: “ The insects will tell you everything, people lie, but insects don't lie.” Murray Marks ---forensic anthropologist, University of Tennessee.

The egg mass – the advantage of a well-The egg mass – the advantage of a well-placed larvaplaced larva

Page 26: “ The insects will tell you everything, people lie, but insects don't lie.” Murray Marks ---forensic anthropologist, University of Tennessee.

The egg mass – the advantage of a well-The egg mass – the advantage of a well-placed larvaplaced larva

Page 27: “ The insects will tell you everything, people lie, but insects don't lie.” Murray Marks ---forensic anthropologist, University of Tennessee.

The egg mass – the advantage of a well-The egg mass – the advantage of a well-placed larvaplaced larva

Page 28: “ The insects will tell you everything, people lie, but insects don't lie.” Murray Marks ---forensic anthropologist, University of Tennessee.

The egg mass – the advantage of a well-The egg mass – the advantage of a well-placed larvaplaced larva

Page 29: “ The insects will tell you everything, people lie, but insects don't lie.” Murray Marks ---forensic anthropologist, University of Tennessee.

The egg mass – the advantage of a well-The egg mass – the advantage of a well-placed larvaplaced larva

Page 30: “ The insects will tell you everything, people lie, but insects don't lie.” Murray Marks ---forensic anthropologist, University of Tennessee.

The egg mass – the advantage of a well-The egg mass – the advantage of a well-placed larvaplaced larva

Page 31: “ The insects will tell you everything, people lie, but insects don't lie.” Murray Marks ---forensic anthropologist, University of Tennessee.

The egg mass – the advantage of a well-The egg mass – the advantage of a well-placed larvaplaced larva

Page 32: “ The insects will tell you everything, people lie, but insects don't lie.” Murray Marks ---forensic anthropologist, University of Tennessee.

The egg mass – the advantage of a well-The egg mass – the advantage of a well-placed larvaplaced larva

Page 33: “ The insects will tell you everything, people lie, but insects don't lie.” Murray Marks ---forensic anthropologist, University of Tennessee.

The egg mass – the advantage of a well-The egg mass – the advantage of a well-placed larvaplaced larva

Page 34: “ The insects will tell you everything, people lie, but insects don't lie.” Murray Marks ---forensic anthropologist, University of Tennessee.

The egg mass – the advantage of a well-The egg mass – the advantage of a well-placed larvaplaced larva

Page 35: “ The insects will tell you everything, people lie, but insects don't lie.” Murray Marks ---forensic anthropologist, University of Tennessee.

The egg mass – the advantage of a well-The egg mass – the advantage of a well-placed larvaplaced larva

Page 36: “ The insects will tell you everything, people lie, but insects don't lie.” Murray Marks ---forensic anthropologist, University of Tennessee.

The egg mass – the advantage of a well-The egg mass – the advantage of a well-placed larvaplaced larva

Page 37: “ The insects will tell you everything, people lie, but insects don't lie.” Murray Marks ---forensic anthropologist, University of Tennessee.

The egg mass – the advantage of a well-The egg mass – the advantage of a well-placed larvaplaced larva

Page 38: “ The insects will tell you everything, people lie, but insects don't lie.” Murray Marks ---forensic anthropologist, University of Tennessee.

The egg mass – the advantage of a well-The egg mass – the advantage of a well-placed larvaplaced larva

Page 39: “ The insects will tell you everything, people lie, but insects don't lie.” Murray Marks ---forensic anthropologist, University of Tennessee.

The egg mass – the advantage of a well-The egg mass – the advantage of a well-placed larvaplaced larva

Page 40: “ The insects will tell you everything, people lie, but insects don't lie.” Murray Marks ---forensic anthropologist, University of Tennessee.

The egg mass – the advantage of a well-The egg mass – the advantage of a well-placed larvaplaced larva

Page 41: “ The insects will tell you everything, people lie, but insects don't lie.” Murray Marks ---forensic anthropologist, University of Tennessee.

The egg mass – the advantage of a well-The egg mass – the advantage of a well-placed larvaplaced larva

Page 42: “ The insects will tell you everything, people lie, but insects don't lie.” Murray Marks ---forensic anthropologist, University of Tennessee.
Page 43: “ The insects will tell you everything, people lie, but insects don't lie.” Murray Marks ---forensic anthropologist, University of Tennessee.

The Role of Dipterans (true flies) in The Role of Dipterans (true flies) in Determining Time of DeathDetermining Time of Death

• Flies are Flies are ectothermsectotherms, thus growth , thus growth rates for a particular species are rates for a particular species are primarily a function of time and primarily a function of time and temperature.temperature.

Page 44: “ The insects will tell you everything, people lie, but insects don't lie.” Murray Marks ---forensic anthropologist, University of Tennessee.

The Role of Dipterans (true flies) in The Role of Dipterans (true flies) in Determining Time of DeathDetermining Time of Death

• Flies are Flies are ectothermsectotherms, thus growth , thus growth rates for a particular species are rates for a particular species are primarily a function of time and primarily a function of time and temperature.temperature.

• If you know the temperature and the If you know the temperature and the size of the larvae (maggot), you can size of the larvae (maggot), you can determine the time of initial determine the time of initial colonization.colonization.

Page 45: “ The insects will tell you everything, people lie, but insects don't lie.” Murray Marks ---forensic anthropologist, University of Tennessee.
Page 46: “ The insects will tell you everything, people lie, but insects don't lie.” Murray Marks ---forensic anthropologist, University of Tennessee.
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The Adult Fly -- FeedingThe Adult Fly -- Feeding