Test for Linkage Progreny Progreny Counts Counts AA BB AA BB aa bb aa bb (P) (P) H 0 : The loci are NOT : The loci are NOT linked. linked. calc > crit H 0 can be rejected. The loci do not assort independently. Aa B Bb aa bb aa bb Aa Bb Aa bb bb aa aa Bb aa aa bb bb 140 140 110 110 115 115 135 135 Obs: 140 Obs: 140 Exp: Exp: 255(250/500) 255(250/500) = 127.5 = 127.5 Obs: 115 Obs: 115 Exp: Exp: 255(250/500) 255(250/500) = 127.5 = 127.5 Obs: 110 Obs: 110 Exp: Exp: 245(250/500) 245(250/500) = 122.5 = 122.5 Obs: 135 Obs: 135 Exp: Exp: 245(250/500) 245(250/500) = 122.5 = 122.5 L O C U S S 1 1 A a B B b LOCUS 2 LOCUS 2 Total Total Total Total 2 5 5 2 5 5 2 4 5 2 4 5 5 0 0 5 0 0 2 5 0 2 5 0 2 5 0 2 5 0 calc = 5.002 df = (df rows ) x (df columns ) = 1 crit = 3.841
10
Embed
Test for Linkage Progreny Counts AA BB aa bb aa bb(P) H 0 : The loci are NOT linked. calc > crit H 0 can be rejected. The loci do not.
This document is posted to help you gain knowledge. Please leave a comment to let me know what you think about it! Share it to your friends and learn new things together.
Transcript
Test for Linkage
Progreny Progreny CountsCounts
AA BBAA BB aa bbaa bb(P)(P)
HH00: The loci are NOT linked.: The loci are NOT linked.
calc >
crit
H0 can be rejected. The loci do not assort independently.
AAaa B Bbb aa bbaa bb
AAaa BBbbAAaa bbbb aaaa BBbbaa aa bbbb
140140110110115115135135
Obs: 140Obs: 140
Exp: Exp: 255(250/500)255(250/500)
= 127.5= 127.5
Obs: 115Obs: 115
Exp: Exp: 255(250/500)255(250/500)
= 127.5= 127.5
Obs: 110Obs: 110
Exp: Exp: 245(250/500)245(250/500)
= 122.5= 122.5
Obs: 135Obs: 135
Exp: Exp: 245(250/500)245(250/500)
= 122.5= 122.5
LLOOCCUUS S
1 1
AA
aa
B B bbLOCUS 2LOCUS 2
Total Total
Total Total
2 5 5 2 5 5
2 4 5 2 4 5
5 0 0 5 0 0
2 5 0 2 5 0
2 5 0 2 5 0
calc = 5.002
df = (dfrows) x (dfcolumns) = 1
crit = 3.841
Molecular Basis forRelationship between Genotype and Phenotype
DNA
RNA
protein
genotype
function
organismphenotype
DNA sequence
amino acidsequence
transcription
translation
One Gene/One Enzyme Hypothesis (Beadle and Tatum)One Gene/One Enzyme Hypothesis (Beadle and Tatum)
Wild-type Wild-type NeurosporaNeurospora crassacrassa (red bread mold) is (red bread mold) is prototrophic.prototrophic.
Prototrophs are able to grow Prototrophs are able to grow on minimal medium.on minimal medium.
Minimal medium contains only Minimal medium contains only inorganic salts, simple carbon inorganic salts, simple carbon source, and water.source, and water.
Auxotrophs require nutritional Auxotrophs require nutritional supplement(s) for growth.supplement(s) for growth.
One Gene/One Enzyme One Gene/One Enzyme Hypothesis (Beadle and Tatum)Hypothesis (Beadle and Tatum)
Conidia are mutagenized Conidia are mutagenized with X-ray.with X-ray.
Possible mutants are Possible mutants are crossed to wild-type and crossed to wild-type and individual ascospores are individual ascospores are cultured on complete cultured on complete (permissive) medium.(permissive) medium.
Cultures are plated on Cultures are plated on minimal medium and minimal medium and auxotrophs are isolated.auxotrophs are isolated.
Auxotrophs are tested for Auxotrophs are tested for nutritional requirement.nutritional requirement.
One Gene/One Enzyme Hypothesis (Beadle and Tatum)One Gene/One Enzyme Hypothesis (Beadle and Tatum)
Each requirement for a specific nutrient (mutant Each requirement for a specific nutrient (mutant effect) followed Mendelian pattern of inheritance.effect) followed Mendelian pattern of inheritance.
Beadle and Tatum deduced that each mutant was Beadle and Tatum deduced that each mutant was deficient for an essential enzyme in a biosynthetic deficient for an essential enzyme in a biosynthetic pathway.pathway.
compound X compound Y compound Z
enzyme A enzyme B
compound X compound Y
enzyme A
A_ B_
A_ bb
Not All Gene Products are Enzymes!Not All Gene Products are Enzymes!
One Gene/One EnzymeOne Gene/One Enzyme
One Gene/One PolypeptideOne Gene/One Polypeptide(structural proteins, subunits)(structural proteins, subunits)
One Gene/One ProductOne Gene/One Product(rRNA, tRNA)(rRNA, tRNA)
Mutants of Arginine Biosynthetic PathwayMutants of Arginine Biosynthetic Pathway