江西农业大学学报 2011 , 33 ( 2 ): 0355 - 0359 http: / / xuebao. jxau. edu. cn Acta AGriculturae Universitatis JianGxiensis E - mail: ndxb7775@ sina. com 生 防 菌 弗氏链 霉菌 S - 221 所 产 抗 菌 物 质的 抗 菌活性 测定 倪国荣 1, 2 , 潘晓华 1 , 张智平 2 , 涂国全 2* ( 1. 江西农业大学 作物生理生态与遗传育种教育部重点实验室 / 农业部双季稻生理生态与栽培重点开放实验室 / 江 西省作物生理生态与遗传育种重点实验室,江西南昌 330045; 2. 江西农业大学 生物科学与工程学院,江西 南昌 330045) 摘要: 对弗氏链霉菌 S - 221 所产抗菌物质的抗菌活性进行研究, 通过弗氏链霉菌 S - 221 所产抗菌物质对 7 种 细菌和 10 种植物病原真菌的抗菌活性测定, 抗菌活性物质对被检测的大肠杆菌 7 种细菌最低抑菌质量浓度 为: 0.098 ~3.130 mG/L; 抗菌活性物质分别对水稻纹枯病等供试 10 种病原真菌的 EC 50 为 0. 39 ~ 4. 83 mG/L 和 EC 90 范围为 8. 17 ~ 28. 44 mG/L。实验结果显示弗氏链霉菌 S - 221 所产抗菌物质具有广谱和强效的抗菌 性, 具有较广的应用前景。 关键词: 弗氏链霉菌 S - 221; 抗菌活性; 植物病原菌 中图分类号: Q939. 92 文献标志码: A 文章编号: 1000 - 2286( 2011) 02 - 0355 - 05 Determination of the Antimicrobial Activity of Antibacterial Substances Produced by Biocontrol Bacteria Streptomyces fradiae var S - 221 NI Guo-ronG 1, 2 , PAN Xiao-hua 1 , ZHANG Zhi-pinG 2 , TU Guo-quan 2* ( 1. Crop PhysioloGy and EcoloGy and Genetics and BreedinG with the Ministry of Education Key Laborato- ry, Double - crop Rice EcoloGy and PhysioloGy and Cultivation with Ministry of AGriculture Key Open Labora- tory Crop PhysioloGy, JAU, NanchanG 330045 , China; 2. Bioscience and BiotecnoloGy ColleGe, JAU, NanchanG 330045 , China) Abstract: To explore the antimicrobial activity of antibacterial substances produced by Streptomyces fradi- ae var S - 221 . The antimicrobial activity of antibacterial substances produced by Streptomyces fradiae var S - 221 on 7 kinds of bacteria and 10 kinds of plant pathoGens was determined. The ranGe of MIC to Escherichia coli at on 7 kinds of bacteria is from 0. 098 to 3. 130 mG /L, the ranGe of EC 50 on 10 kinds of plant pathoGens is from 0. 39 ~ 4. 83 mG /L and the ranGe of EC 90 on 10 kinds of plant pathoGens is from 8. 17 ~ 28. 44 mG /L. The inhibitory action of antibacterial substance produced by Streptomyces fradiae Var S - 221 is powerful and the pediGree of antimicrobial is wide. Key words: Streptomyces fradiae var S - 221 ; Antimicrobial activity ; plant pathoGens 植物病虫害是农业生产的大敌, 据统计, 全球每年植物因遭受病虫害而造成的减产损失平均为总产 量的 45% [ 1-2 ] , 尤其是一些重要的经济作物, 如稻谷、 小麦、 土豆、 棉花和咖啡等 [ 3 ] , 给各国经济都造成 收稿日期: 2010 - 09 - 26 修回日期: 2011 - 02 - 15 基金项目: 国家十一五科技支撑计划项目( 2006BAD02A04) 和江西省研究生创新专项资金项目( YC08A054) 作者简介: 倪国荣( 1983—), 男, 博士生, 主要从事微生物与作物高产研究, E - mail: nGr314@ 163. com; * 通讯作者: 涂国全, 教授, E - mail: tuGuoquan@ 263. net。
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江西农业大学学报 2011,33( 2) : 0355 - 0359 http: / / xuebao. jxau. edu. cnActa Agriculturae Universitatis Jiangxiensis E - mail: ndxb7775@ sina. com
关键词:弗氏链霉菌 S - 221;抗菌活性;植物病原菌中图分类号: Q939. 92 文献标志码: A 文章编号: 1000 - 2286( 2011) 02 - 0355 - 05
Determination of the Antimicrobial Activity of Antibacterial SubstancesProduced by Biocontrol Bacteria Streptomyces fradiae var S - 221
NI Guo-rong1,2,PAN Xiao-hua1,ZHANG Zhi-ping2,TU Guo-quan2*
( 1. Crop Physiology and Ecology and Genetics and Breeding with the Ministry of Education Key Laborato-ry,Double - crop Rice Ecology and Physiology and Cultivation with Ministry of Agriculture Key Open Labora-tory Crop Physiology,JAU,Nanchang 330045,China; 2. Bioscience and Biotecnology College,JAU,Nanchang330045,China)
Abstract: To explore the antimicrobial activity of antibacterial substances produced by Streptomyces fradi-ae var S - 221 . The antimicrobial activity of antibacterial substances produced by Streptomyces fradiae var S -221 on 7 kinds of bacteria and 10 kinds of plant pathogens was determined. The range of MIC to Escherichiacoli at on 7 kinds of bacteria is from 0. 098 to 3. 130 mg /L,the range of EC50 on 10 kinds of plant pathogensis from 0. 39 ~ 4. 83 mg /L and the range of EC90 on 10 kinds of plant pathogens is from 8. 17 ~ 28. 44 mg /L.The inhibitory action of antibacterial substance produced by Streptomyces fradiae Var S - 221 is powerful andthe pedigree of antimicrobial is wide.
Key words: Streptomyces fradiae var S - 221; Antimicrobial activity ; plant pathogens
( 2) 弗氏链霉菌 S - 221 所产抗菌活性物质对细菌和植物病原真菌的都表现出了良好的抑制效果,且细菌较植物病原真菌对抗菌物质更敏感。广谱的抗菌谱和较强的抗菌活性,为其开发生物农药提供了依据。
( 3) 目前对弗氏链霉菌研究表明,现已发现的弗氏链霉菌产生的抗菌物质主要有泰乐菌素[12]和新霉素[13 - 14],弗氏链霉菌 S - 221 产生的抗菌物质的抗菌谱与已发现的泰乐菌素和新霉素不同,其具体成分和抑菌机理需有待于进一步研究。
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第 2 期 倪国荣等:生防菌弗氏链霉菌 S - 221 所产抗菌物质的抗菌活性测定
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