Jan 17, 2016
hematocrit, HctHt,HCT packed cell volume, PCV
layer after blood centrifugated
plasm
platlet white cellreduced hemoglobin oxygen red cell
Red Blood Cells
1Wintrobe2.microhematocrit
3 Hct = RBCMCV
1 ICSHWintrobe WHO 2
1. 2. Hct
Wintrobe0.40.540.370.47 0.470.040.420.05 132
mean corpuscular volume, MCVfl(mean corpuscular hemoglobin, MCH)pg(mean corpuscular Hemoglobin concentration, MCHC)g/L
1 1 Hct MCV= = fl RBC 2 Hb MCH= = pg RBC 3 Hb MCHC= = Hct
2 MCVRBCHbMCHMCHCMCH=Hb/RBCMCHC=Hb/RBCMCV
1.MCV MCV360flMCV600mg/LMCVCV1%CV10%2.MCH MCH3.MCHC MCHCHctHb
1. 2. 333.XBMCHC33%,2%3%60
MCV 8092flMCH 2731pg MCHC320360g/L MCV 80100flMCH 2734pgMCHC320360g/L
(red blood cell volume distribution width, RDW)CV
RDWMCV RDWRDW 11.6%14.6%
1 1983BessmanMCVRDW 2.iron deficiency anemia, IDA RDW95% 3. RDW-RDW
reticulocyte, RetRETribomucleic acidRNAbrilliant cresyl blue79mHeilmyer5ICSH4Heilmyer 0
1 2 -YRNAflow cytometryFCM 3
1
reticulocyte
Miller
2 high fluorescent ratioHFRmiddle fluorescent rationMFRlow fluorescent ratioLFR
3 RetRctRct%mean reticulocyte volume, MCVrreticulocyte hemoglobin distribution width , HDWrreticulocyte hemoglobin concentration ,HCRmean reticulocyte hemoglobin concentration , MCHCrLFRMFRHFRreticulocyte maturity index RMIRMI=MFR+HFR/LFR100
4 12=/L
3reticulocyte production index, RPI2d Hct 1 RPI= Ret% Hct 2Hct45%40%
1 Miller2 10 00050 000Howell-Jolly 1 0.0080.022575109/L0.020.062
0.20.3IV0.70.8III
1 1Ret 2
2 Ret3
RNA
basiphilic stippling count1000
50%= 510 310-4
1. 2.
erythrocyte sedimentation rateESR310min40min
1Westergren 1hmm
2 National Clinical Committee of Laboratory Standardization NCCLS2000EDTA-K21HtH-t2201000
1 ICSH
2
1
2 3 45
50020mm/h030mm/h>85030mm/h042mm/h010mm/h
1132 123d 2
345 62
white blood cell WBC leukocyteLEUgranulocyteGRANlymphocyteLmonocyteM
1 2
1 1/5001 2
WBCWBC
WBC/HP WBC109/L
24 47 46 79 610 1012 1012 ( 13l8 )
2.(routine checking standard ,RCS) 3. V Xi Xm
4. 22 rx1x2125. (CV) n x1x2
(4l0) 109/L (1520) 109/L62 (1112) 109/L
(differential countDC) 1
2
1. 1 2 2. 10020095%
3. 4. National Clinical Committee of Laboratory StandardizationNCCLSEDTAK3 Romanowaky2004200(800)
% 109/L
15 (0.040.5) 5070 (27) 0.55 (0.020.5) 01 (01) 2040 (0.84) 38 (0.120.8)
(neutrophilN) (mitotic pool)(maturation pool)(storage pool)(circulating pool)marginating pool
granulocyte pool
10 Bone marrow 10h 1~2Mitotic poolMaturation poolStorage poolMargin poolCirculating pool
(1)(neutrophilia)70%7l09/L1) 69d4530%
2)
(1020) l09/L(2030) l09/L
()1236h ,10l09/L12d1
12h(1020) l09/L
MICMM
(acute myelogenous leukemiaAML)(acute lymphocytic leukemiaALL)(1050) l09/L100l09/L
10%(100600) l09/L 50l09/L
100l09/L10%
2neutropenia 1.5l09/Lgranulocytopenia0.5l09/L(agranulocytosis)
12(aleukemic leukemia)
34systemic lupus erythematosusSLE5
3
1)shift to the left5%
(5%)10%25%
2)shift to the right53%
2. 3. (basophilB) 1basophilia0.05109/L1IgE2 3 2(basopenia)
4. 1(lymphocytosis) 4109/L 7.2109/L4 9109/L 1
2
2(lymphopenia)
5. 1(monocytosis)0.8109/L 12 2 2
(eosinophilE )
1 2
(0.050.5)109/L 1
2(eosinophilia) 0.5109/L (1)10% (2) (3)
(4) (5) (6)
3(eosinopenia)
4 (1) (2) (3)adrenocorticotropic hormoneACTHACTH 25mg 0.1%0.5mlACTH
50%50%80%l00%50%(Addison)4%
1 Romanovsky 2
1. 4
/
2.11 Dhle
2
3(Auer body)
4PelgerHuet2()
ChediakHigashi1025m
Alder-Reilly
MayHegglinDhle
21abnormal lymphocyteTDowney3I()() ()
23
3plasma cell
1MottMott cell
2flame cellIgA
3Russell (Russell body) 23m