-
Ángel Pereira Rodríguez
Structural characterization of the β-galactosidase
from Kluyveromyces lactis and expression and
directed evolution of β-galactosidases with high
biotechnological interest
Facultade de Ciencias Departamento de Bioloxía Celular e
Molecular
Área de Bioquímica e Bioloxía Molecular
Director Manuel Becerra Fernández
2012
-
3
El presente trabajo, Structural characterization of the
β-galactosidase from Kluyveromyces lactis and expression and
directed evolution of β-galactosidases with high biotechnological
interest presentado por Don Ángel Pereira Rodríguez para aspirar al
grado de Doctor en Biología, ha sido realizado bajo mi dirección en
el Departamento de Biología Celular y Molecular de la Universidad
de A Coruña. Revisado el texto, estoy conforme con su presentación
para ser juzgado.
A Coruña, 5 de Junio de 2012
VºBº Dr. Manuel Becerra Fernández
Profesor de Bioquímica y Biología Molecular
-
5
El autor de este trabajo ha disfrutado durante la realización de
esta tesis de una beca predoctoral concedida por la Universidade da
Coruña (Enero de 2007 - Diciembre de 2007), un contrato predoctoral
concedido por la Universidade da Coruña (Enero 2008 – Diciembre
2008), y un contrato asociado al programa María Barbeito concedido
por la Xunta de Galicia (Enero 2009 – Diciembre 2010). Parte del
capítulo 4 de esta tesis ha sido realizado en el Departamento de
Engenharia Biológica da Universidade do Minho (Braga, Portugal)
durante una estancia de 3 meses. La realización de este trabajo ha
sido posible gracias a la financiación obtenida a través de los
proyectos 09REM001E, 07TAL006E y PGIDIT03BTF10302PR concedidos por
la Xunta de Galicia y gracias además a la financiación general del
laboratorio a través del "Programa de consolidación de C.E.O.U." de
la Xunta de Galicia durante los años 2008 a 2011, ambos
cofinanciados por FEDER (CEE).
-
7
A mis padres
“La inspiración existe, pero tiene que encontrarte
trabajando”
Pablo Picasso
“If you are good, and you like it... Go for it!!!” - Ei-ichi
Negishi
(2010 Nobel Prize in Chemistry) - 26-04-2012 (Cidade da Cultura
– Santiago de Compostela)
-
Acknowledgements
9
Acknowledgements
Para los agradecimientos necesito expresarme en español, porque
sin duda
uno se expresa mejor en el idioma con el que piensa y siente, y
en este caso ha
sido más la pasión por la ciencia que lo puramente experimental
y
procedimental lo que me ha empujado a realizar esta tesis
doctoral.
Una tesis doctoral que no es “al uso” por muchos motivos, pero
el más
evidente es que para cuando esté defendiendo ante el tribunal
esta tesis habrán
pasado ¡¡¡8 años!!! Sí, parecen muchos años, pero cuando estás
trabajando en
ciencia el tiempo pasa muy deprisa…
Es por ello que tengo muchísima gente a la que agradecer y que
forman parte
de esta tesis, que además de ser un dossier de artículos
científicos, lleva
asociado mucho de amistad/amor/colaboración/etc.
Para empezar por el principio, he de decir que tengo mucho que
agradecerle a
la catedrática Mª Esperanza Cerdán Villanueva, puesto que si no
me hubiese
ofrecido el puesto de investigador allá por 2004, esto
directamente no habría
sido posible.
Lo mismo me pasa con la catedrática Mª Isabel González Siso, a
la que he de
agradecerle que comenzara por los años 90 a trabajar en una
proteína, la β-
galactosidasa, que ha sido el centro sobre el que giró mi
trabajo desde que
entré en el laboratorio de Bioquímica, y por tanto sin ese
proyecto
seguramente tampoco estaría ahora escribiendo estos
agradecimientos.
Mención especial en este apartado tiene que tener por muchos
motivos,
Manuel Becerra Fernández, entre ellos sin duda el de ser la
persona que me ha
enseñado prácticamente todo lo que sé sobre bioquímica, y
sobretodo que me
ha apoyado sin descanso desde el minuto número uno, a pesar de
que el
primer año fue un año muy duro porque no salía nada de lo que se
pretendía,
y sin embargo él no paró nunca de animarme a seguir con
ello.
No sólo por esto le tengo que estar agradecido, sino también por
cómo es
como persona, ya que su modestia y su optimismo te embargan, y
te hacen
olvidar todo lo negativo que puedes ver. Quizás de lo más
importante y de los
que tengo muy gratos recuerdos es de su labor de psicólogo,
puesto que supo
darme una palmada en la espalda cuando las cosas no iban bien y
ponerme los
pies en la Tierra cuando me parecía que todo iba sobre ruedas,
es decir, te
-
Acknowledgements
10
hace mantenerte siempre en un equilibrio emocional para que no
pierdas la
ruta hacia tu destino.
Por todo ello y mucho más, muchísimas gracias y nunca olvidaré
esta etapa
como tutor (si estás leyendo esta tesis y tu tutor es Manuel, me
alegro de
decirte que te ha tocado la lotería).
No puedo dejar de lado ni mucho menos, a todas las profesoras
del
laboratorio, puesto que cada una con su personalidad y su
especialidad dentro
de la bioquímica me han sabido ayudar siempre que las he
necesitado, por
tanto gracias a Ana Mª Rodríguez Torres, Mª Ángeles Freire
Picos, Esther
Rodríguez Belmonte y Mónica Lamas Maceiras (mención para esta
última por
aguantarnos sin rechistar en el laboratorio a todos los
doctorandos, alumnos
de máster, etc; por esos debates sobre los temas de actualidad
en cada
momento, por ser tan cercana, y por las múltiples risas que nos
hemos
echado).
Agradecido a todos y cada uno de los compañeros de laboratorio,
pues de
todos traté de aprender algo.
Entre los que vinieron de fuera tienen mención especial y les
estoy
enormemente agradecido:
Marcin Krupka: eres un fuera de serie, sonrisa permanente y
retranca gallega
dentro de un polaco, lo nunca visto. Transmites un “buen rollo”
que proyecta
luz también a todos los que te rodean y es muy de agradecer.
Nunca olvidaré
el primer seminario sobre ubiquitinación, creo que fue la
primera y única vez
que alguien me pidió información a mayores de la explicada en el
propio
seminario, para seguir estudiando más a fondo lo explicado
(puede parecer
una chorrada, pero en realidad este detalle resume muy bien el
nivel de
implicación de Marcin en su trabajo).
Saúl Nitsche Rocha: según los estudios científicos hasta hoy el
único brasileño
al que no le gusta el fútbol, y un fuera de serie en
fermentaciones y HPLC, es
más, prácticamente todo lo que sé sobre estas dos materias me lo
enseñó él.
Aparte de lo increíblemente profesional que es, además es una
gran persona
(cariñoso con sus amigos y muy optimista) y no olvidaré nunca
las discusiones
sobre F1 para desconectar en el laboratorio.
Un párrafo aparte se merecen, porque también pusieron su granito
de arena,
aquellos tres “veteranos” que me encontré cuando empecé en el
laboratorio y
-
Acknowledgements
11
de los que aprendí mucho y por tanto vayan por delante también
mis
agradecimientos:
Moisés Blanco Calvo: trabajador incansable, modesto y que
siempre te echaba
una mano cuando la necesitabas. Muchas risas y debates también
que
quedarán para siempre en mi memoria.
Nuria Tarrío Yañez: optimista por definición, valiente y
decidida, que nos
hacía sumergirnos en sus ideas y sus mundos, para poder
desconectar un
poco.
Laura Nuñez Naveira: nunca paraba, pasión por la ciencia elevada
al infinito, y
precisa en todo lo que hacía. Un ejemplo de hasta que punto la
pasión por lo
que haces es esencial y una fuera de serie en cualquier técnica
de la que le
preguntaras (siempre había probado múltiples posibilidades hasta
dar con la
óptima).
Otra mención especial para las técnico de laboratorio con las
que conviví en el
laboratorio: empezando por Aida Sanchéz López (“always smiling
woman”),
Elisa Santiago Beloso - “Lisi” (muy cariñosa y una cocinera
espectacular) y
María (otra enorme cocinera y siempre ayudándote en lo que
necesitaras).
No me quiero olvidar de darle las gracias también a Cristina
Trillo, cuyos
trabajos previos en la β-galactosidasa hicieron posible mi
primer artículo, y
fueron además el punto de inicio de la cristalización de la
β-galactosidasa.
Otro párrafo específico se merece Raquel Castro Prego (DEP).
Empezamos el
mismo día en el laboratorio, los dos bastante perdidos, y
aquellas
conversaciones que teníamos sobre nuestras dudas iniciales en el
campo de la
investigación, nunca las olvidaré. Se hacía querer, trabajaba
muchísimo, era
muy modesta, y tenía un don para la investigación (no recuerdo
el número de
construcciones que consiguió en los primeros meses de trabajo
pero seguro
que era de record). Siempre estarás en nuestro recuerdo.
Agradecimientos también a Ángel Vizoso Vázquez (otro grande
dentro del
laboratorio, trabajador incansable y otro con un don innato para
la
investigación, encantado de compartir contigo debates sobre
todo
futbolísticos), Agustín, Fátima, Ana García Leiro (muchos
debates sobre la
vida en general), Olalla (las discusiones que manteníamos y el
curso en la
Costa da Caparica inolvidables), María, Elena Canto, Silvia
Rodríguez
Lombardero, Pilar Soengas (inolvidable también tu paso por el
laboratorio,
-
Acknowledgements
12
llegando para dar aire fresco y aprendiendo las nuevas técnicas
tan rápido que
al final eramos nosotros los que aprendíamos de ti, además una
gran persona.
Muy agradecido por la visita que me hicísteis cuando estaba en
Braga y esa
comida en O Escondidinho), Marcos Poncelas (bioquímico de los
pies a la
cabeza, desde el instituto tenía claro que lo tuyo era la
bioquímica), María
Teresa, Mónica Rey, Rebeca Santiso, Margherita Scarpa, Silvia
Remeseiro,
Emilie Dambroise, Silvia Seoane, Silvia Díaz, Susana Pedraza,
Arantxa… y un
largo etcétera porque seguro que me dejo a algún@.
Dejo para el final entre los compañeros de laboratorio con el
que compartí
más tiempo que con mi familia o mi novia: Rafael Fernández
Leiro.
Mereces un apartado aparte también por múltiples motivos, entre
los cuales:
Ser mi brújula en mis inicios (aquellos dos meses que estuviste
en verano
marcaron sin duda un camino a seguir), colaborar desde el
principio por el
bien común y tratar de hacer piña para lograr sacar la cabeza en
un mundo
muy competitivo, por las miles de horas de debates de todo tipo
incluídos por
supuesto las divagaciones sobre la genética, la proteómica, …
mientras
hacíamos las purificaciones, fermentaciones, … y por supuesto
las disputas
sobre informática.
Has sido un ejemplo de pasión por el trabajo y de querer seguir
creciendo
siempre, de persona precisa, ordenada y organizada, y un colega
de laboratorio
ideal hasta el último día, por lo que te estoy enormemente
agradecido.
También gracias a todos los profesores y doctorandos de los
laboratorios de la
facultad con los que he interactuado por facilitarme en cada
momento lo que
necesitara para realizar mis experimentos; así como al personal
de limpieza y
de seguridad (siento haber sido tan pesado de madrugada debido a
los
fermentadores) que siempre son unos olvidados pero con los que
también
había un trato muy cercano que se agradecía. Mención aparte para
la gente de
mantenimiento, con los que logré convertir los dos fermentadores
de los 90
en dos Delorean con condensador de fluzo y todo ;).
No me olvidaré nunca tampoco de mis inicios cuando
compatibilizaba mi
trabajo en la UDC con ATENTO SA y STREAM SAI, donde tuve la
suerte
de coincidir con grandes compañeros que me hacían desconectar
del
laboratorio y con los que me he reído muchísimo.
-
Acknowledgements
13
Tampoco puedo olvidar a mis amigos de As Pontes de toda la vida,
y mis
compañeros en Ponferrada, por hacerme desconectar y hacerme ver
que no
todo era bioquímica, fermentaciones, etc.
Otro apartado especial de los agradecimientos se lo merecen el
profesor Jose
Antonio Couto Teixeira y Lucilia Domingues, por invitarme a
realizar una
estancia en el Departamento de Engenharia Biológica (Campus de
Gualtar -
Universidade do Minho - Braga) y por darme todas las facilidades
para poder
aprovechar una estancia tan corta como la que tuve sin apenas
conocerme,
muito obrigado de corazón por la hospitalidad.
De Portugal (país del que estoy enamorado desde hace años) me
acordaré
siempre de los compañeros que tuve en la planta piloto (en
especial André
Mota, que me buscó piso, me enseñó las costumbres y la
gastronomía
portuguesas, con el que mantuve debates muy interesantes, y
aprendí de él
todo lo que sé sobre airlifts y cultivos continuos) y a los
compañeros del
LEMM que fue mi laboratorio base, y en donde tienen una mención
especial
Carla Oliveira que me facilitó la vida mucho los primeros días,
Pedro
Guimaraes que me facilitó mucho mi trabajo como especialista que
es en la β-
galactosidasa y Francisco Pereira “Xico” al que le debo mucho ya
que aparte
de la muchísima ayuda que me prestó, fueron muchas horas de
risas y
conversaciones a últimas y primeras horas del día, un ejemplo
también de
trabajador incansable y una gran persona con mayúsculas, muito
obrigado pa.
También una mención especial tienen dos profesores con los que
congenié en
tan sólo unos minutos de conversación en el curso de la UIMP
sobre
“Valorización de residuos lácteos” allá por el 2009: Jesús
Ernesto Hernández
Ortega y Athanasios Koutinas.
Otra mención especial se la merece Benigno Pereira Ramos por
romper con el
tradicional miedo de los empresarios a trabajar con las
universidades y por el
haber subvencionado parte de la investigación que he realizado
durante los
aproximadamente 4 últimos años y que no puede aparecer en esta
tesis por
motivos de confidencialidad (Queizuar SL – Queixerías Bama).
Aquí también debo agradecer a todo el personal con el que tuve
el placer de
trabajar en el CITI con mención especial para el catedrático
Lorenzo Pastrana
Castro, María Luisa Rúa Rodríguez y los alumnos de su
laboratorio y
circundantes por la hospitalidad y ofrecerme todo lo que tenían
para que el
trabajo que realicé saliese adelante, y también para Elisa
González por toda la
ayuda que necesité para estudiar las tripas del fermentador del
CITI.
-
Acknowledgements
14
También gracias al Instituto Danone por la beca que me
concedieron, y a la
UDC y a la Xunta por ese mismo motivo. Gracias también por el
premio al
mejor poster al equipo docente del curso avanzado “Hands on” de
la Costa de
Caparica.
En cuanto a los más allegados, tengo mucho que agradecer en
todos los
sentidos y también forman parte de esta tesis en gran medida mis
padrinos y
madrinas, así como a mi prima Montse por dejarme “okupar” su
casa y
ayudarme desde que era un crío, y a mi primo Suso, que ha sido y
es mi
hermano mayor.
No me puedo olvidar de la persona que más me apoyó sin duda
alguna en
todo momento e incondicionalmente en esta fase de mi vida, que
fue Elena
Bausá Cano, sin cuya ayuda y cariño esta tesis seguramente no
habría sido
posible, muchas gracias de corazón, un pedazo grande de esta
tesis te
pertenece por hacerme tan feliz. Además este agradecimiento
quiero hacerlo
extensible a su familia la cual también fue un apoyo esencial en
esta época de
mi vida (un besito para Paula).
Para la parte final agradecimientos a mi hermanita Iria y a
Hugo, por
apoyarme siempre también en lo que he hecho, ayudarme a
desconectar y por
hacerme el tío más afortunado del mundo dando vida a mi sobrina
Vania que
es la niña más preciosa de este mundo.
Y para finalizar los agradecimientos, las personas a las que más
les debo en
esta vida, que son mis fans número 1, y que siempre han confiado
en mí en
todas mis decisiones, que no son otros que mis padres: Mª Albina
Rodríguez
Castro y Ángel B. Pereira González, GRACIAS CON MAYÚSCULAS.
Después de ocho años, en los que no ha sido fácil ni mucho menos
(han sido
muchos festivos, fines de semana, madrugadas, sin apenas
vacaciones,…) creo
que ha merecido mucho la pena y espero que el trabajo de esta
tesis pueda
valer para que otros sigan apostando por esta profesión que
necesita más
temprano que tarde ser dignificada, profesionalizada y ocupar un
lugar
estratégico en nuestro país sin importar los colores políticos,
puesto que es de
la educación y de la investigación de lo que depende la
capacidad competitiva
de un país. Espero no perder nunca I melme ter i nolme.
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15
Contents
-
17
Contents
Abstract / Resumen / Resumo 19
General Introduction 27
Objectives 41
Outline of this thesis 45
Chapter 1 49
Kluyveromyces lactis β-galactosidase crystallization using
full-factorial
experimental design
Chapter 2 65
Crystallization and preliminary X-ray crystallographic analysis
of β-
galactosidase from Kluyveromyces lactis
Chapter 3 77
Structural basis of specificity in tetrameric Kluyveromyces
lactis β-galactosidase
Chapter 4 105
A hybrid Kluyveromyces lactis-Aspergillus niger
β-galactosidase
Chapter 5 153
Heterologous expression and directed evolution of Aspergillus
niger β-
galactosidase
Concluding remarks 177
References 181
Appendix I – Material and Methods 193
Appendix II – Resumen 245
Appendix III – Curriculum vitae 257
-
19
Abstract
-
Abstract
21
β-galactosidases are hydrolase enzymes that catalyze the
hydrolysis of β-
galactosides into their monosaccharides. Due to this ability
these proteins are
very important in food, clinical and pharmaceutical
industries.
In this thesis the Kluyveromyces lactis β-galactosidase was
cloned in a
Saccharomyces cerevisiae strain, expressed, purified, and
crystallized. Its free state
structure and its complex with the product galactose were
determined to 2.75
and 2.8 Å, respectively. K. lactis β-galactosidase folds into 5
domains in a
pattern conserved with other prokaryote enzymes solved for GH2
family,
although two long insertions in domains 2 (264-274) and 3
(420-443) are
unique and seem related to oligomerization and specificity. K.
lactis β-
galactosidase tetramer is an assembly of dimers, with higher
dissociation
energy for the dimers than for its assembly, which can explain
that equilibrium
exists in solution between the dimeric and tetrameric form of
the enzyme.
On the other hand, a hybrid K. lactis-Aspergillus niger
β-galactosidase was
constructed, expressed and characterized. The hybrid protein
between K. lactis
and A. niger β-galactosidases increases the yield of the protein
released to the
growth medium and the modifications introduced in the
construction
conferred to the protein biochemical characteristics of
biotechnological
interest. The production of this hybrid K. lactis - A. niger
β-galactosidase was
also tested in a continuous immobilized culture using spent
grains (a by-
product of brewery industry) as an immobilizing material in an
airlift
fermenter.
Finally, different A. niger β-galactosidase constructions were
expressed and
studied in a S. cerevisiae strain and directed evolution
techniques were applied
to modify the optimal pH of the protein to a more neutral
one.
-
Resumen
23
Las -galactosidasas son enzimas hidrolasas que catalizan la
hidrólisis de -
galactósidos en sus monosacáridos correspondientes. Debido a
esta capacidad,
estas proteínas son muy importantes en las industrias
alimentaria, farmacéutica
y clínica.
En esta tesis, la β-galactosidasa de K. lactis fue clonada en
una cepa de
Saccharomyces cerevisiae, expresada, purificada y cristalizada.
Su estructura en
estado libre y la su complejo con el producto galactosa fueron
determinadas a
2,75 y 2,8 Å, respectivamente. La β- galactosidasa de K. lactis
está organizada
en 5 dominios en un patrón que está conservado en otras enzimas
procariotas
de la familia GH2, aunque presenta dos inserciones largas en los
dominios 2
(264-274) y 3 (420-443) que son únicas y parecen estar
relacionadas con su
oligomerización y especificidad. El tetrámero de la
β-galactosidasa de K. lactis
está formado por un par de dímeros, presentando una mayor
energía de
disociación la forma de dímero que su forma tetramérica, lo que
puede
explicar el por qué existe un equilibrio en solución entre la
forma dimérica y
tetramérica de la enzima.
Por otro lado, se ha construido, expresado y caracterizado una
β-galactosidasa
híbrida entre las β-galactosidasas de K. lactis - Aspergillus
niger. La proteína
híbrida entre las β-galactosidasas de K. lactis - A. niger
aumenta el rendimiento
de la proteína secretada al medio y las modificaciones
introducidas en la
construcción le han conferido características bioquímicas de
interés
biotecnológico. Se estudió además la producción de esta proteína
híbrida en
un cultivo continuo inmovilizado utilizando “spent grains” (un
subproducto
de la industria cervecera) como material de inmovilización en un
fermentador
tipo “Airlift”.
Finalmente, se estudió la expresión y producción de la
β-galactosidasa de A.
niger en una cepa de S. cerevisiae, y se usaron técnicas de
evolución dirigida para
modificar el pH óptimo de la proteína hacia un pH más
neutro.
-
Resumo
25
As β-galactosidasas son hidrolasas, enzimas que catalizan a
hidrólise dos β-
galactósidos nos seus monosacáridos correspondentes. Debido a
esta
capacidade, estas proteínas son moi importantes na industria
alimentaria,
farmacéutica e clínica.
Nesta tese, a β-galactosidasa de K. lactis foi clonada nunha
cepa de
Saccharomyces cerevisiae, expresada, purificada e cristalizada.
As estruturas en
estado libre e no complexo co produto galactosa foron
determinadas a 2,75 e
2,8 Å, respectivamente. A β-galactosidasa de K. lactis está
organizada en 5
dominios nun patrón conservado noutras enzimas procariotas da
familia
GH2, pero ademáis presenta dúas insercións longas nos dominios 2
(264-274)
e 3 (420-443) que son únicas e parecen estar relacionadas coa
súa
oligomerización e especificidade. O tetrámero da β-galactosidasa
de K. lactis
está formado por un par de dímeros, tendo unha maior enerxía de
disociación
a forma de dímero que a súa forma tetramérica, o que pode
explicar o por qué
do equilibrio existente en solución entre a forma dimérica e a
forma
tetramérica.
Por outra banda, construiuse unha proteína híbrida entre as
β-galactosidasas
de K. lactis e Aspergillus niger, que foi expresada e
caracterizada. A proteína
híbrida entre as β-galactosidasas de K. lactis – A. niger
aumenta o rendemento
da proteína secretada ao medio e coas modificacións introducidas
na
construción conseguíronse características bioquímicas de gran
interese
biotecnolóxico. Estudouse ademáis a produción da β-galactosidasa
híbrida K.
lactis-A. niger nun cultivo continuo inmovilizado nun
fermentador tipo "Airlift"
usando como material de inmobilización “spent grains” (un
subproduto da
industria cervexeira).
Finalmente, foi estudada a expresión e produción da
β-galactosidasa de A.
niger nunha cepa de S. cerevisiae, e usáronse técnicas de
evolución dirixida para
modificar o pH óptimo da proteína hacia un pH máis neutro.
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27
Introduction
1. β-galactosidases a. What are they? Small summary of E.coli,
K. lactis and A. niger β-
galactosidase.
b. Why β-galactosidase from K. lactis?
c. Why β-galactosidase from A. niger?
d. Which families?
e. Applications?
i. Cheese Whey
ii. Pharmaceutical/Medical Industry
iii. Other Agrofood issues
2. Three-dimensional analysis
3. Directed evolution
-
Introduction
29
β-galactosidases β-galactosidases (sometimes called lactases)
are hydrolase enzymes that
catalyze the hydrolysis of β-galactosides into their
monosaccharides.
There are β-galactosidases from prokaryotes to eukaryotes
(including
humans), and the first sequenced β-galactosidase was the
Escherichia coli β-
galactosidase (Fowler and Zabin 1970) with 1,024 amino
acids1.
This could be stated as the first step in a prolific run into
the study of the β-
galactosidases, and it was and it is at present a model for the
rest of the β-
galactosidases.
Twenty-four years passed until the three-dimensional
β-galactosidase structure
was found (Jacobson et al. 1994), and the information was
essential to
continue studying the β-galactosidases from other organisms like
the
Kluyveromyces lactis β-galactosidase which had been sequenced
only two years
before (Poch et al. 1992).
1.1 Most important β-galactosidases
The most important β-galactosidases due to its biotechnology
potential are:
1. Escherichia coli β-galactosidase (hereafter E. coli
β-galactosidase)
2. Kluyveromyces lactis β-galactosidase (hereafter K. lactis
β-galactosidase
3. Aspergillus niger β-galactosidase (hereafter A. niger
β-galatosidase)
1. E. coli β-galactosidase (EC 3.2.1.23 - P00722)
As it was stated before, its sequence was published in 1970
(Fowler and Zabin
1970) and revealed that the β-galactosidase was composed by
10241 amino
acids and a molecular weight of 116.000 KDa1.
The most important following studies in the E. coli
β-galactosidase (Langley et
al. 1975; Cupples et al. 1990; Jacobson et al. 1994; Roth and
Huber 1996; Roth
and Huber 1996; Huber et al. 2001; Juers et al. 2001; Huber et
al. 2003; Juers et
al. 2003; Roth et al. 2003; Spiwok et al. 2004; Matthews 2005;
Juers et al. 2009;
Lo et al. 2009) determined that the protein is a tetramer of
464,000 KDa, and
each monomer contains five domains, the third of which is an
eight-stranded
α/β barrel that comprises much of the active site.
-
Introduction
30
The five domains are represented in Figure 1:
Figure 1: E. coli β-galactosidase (Domain 1 (1-217) in red,
Domain 2 (218-334) in green, Domain 3 (335-627) in blue,
Domain 4 (628-736) in yellow and Domain 5 (737-1023) in
magenta). Orange and blue spheres are Na and Mg ions
respectively.
As can be seen in the Figure 1, in the tetramer the four
monomers are
grouped around three mutually-perpendicular two-fold axes of
symmetry.
β-Galactosidase has two catalytic activities. First, it
hydrolyzes the
disaccharide lactose to galactose plus glucose. Second, it
converts lactose to
another disaccharide, allolactose, which is the natural inducer
for the lac
operon.
a. How it works
In summary, substrates initially bind in a „„shallow‟‟ mode,
subsequently
moving deeper into the active site so that the glycosidic oxygen
is close
enough to be protonated by Glu-461 (general acid catalysis) and
the galactosyl
anomeric carbon is close enough to contact the nucleophile,
Glu-537. A
carbocation-like transition state forms that collapses into an
a-galactosidic
bond between the carboxyl of Glu-537 and the C1 of galactose.
This first step
of the reaction is called galactosylation (the enzyme becomes
galactosylated).
Upon glycosidic bond cleavage, the first product normally
diffuses away.
Water or an acceptor with a hydroxyl group then enters and is
activated by
Glu-461 via general base catalysis. The galactosyl moiety is
released to this
-
Introduction
31
molecule via a second carbocation-like transition state (the two
transition
states are thought to be similar) to form free galactose or an
adduct having a
galactosidic bond with the acceptor. If the reaction is with
water is called
degalactosylation and if the reaction is with an acceptor is
called
transgalactosylation.
β-Galactosidase requires Mg2+ or Mn2+ for full catalytic
activity, but the exact
role of this ion in catalysis is unclear. The active site also
includes a
monovalent cation (usually either Na+ or K+) important for
activity, which
directly ligates the galactosyl O6 hydroxyl during catalysis.
The two ion sites
are situated a few A° apart in the active site, both very near
to an interface
between two domains of the protein.
Crystal structure and site directed mutagenesis experiments have
shown that
His-418, along with Glu-416 and Glu-461 (the acid/base catalyst)
are
ligands to the Mg2+ at the active site. Besides ligating the
Mg2+ ion, His-418 is
one of several residues that together form an opening that
guides substrates
into the binding site , and it is thought to contact the
aglycone moeity of the
substrate, pointing to a possible role in the formation of
allolactose. His-418 is
also close to Glu-461, and so very likely directly impacts the
properties of that
important catalytic residue.
2. K. lactis β-galactosidase (EC 3.2.1.21 - P00723)
Its sequence was published in 1992 (Poch et al. 1992) and
revealed that the β-
galactosidase was composed by 1025 amino acids and a molecular
weight of
117.618 KDa.
At present, no three-dimensional structure has been published by
any research
group, despite of the importance of this protein in agrofood
and
pharmaceutical/medical industries2.
The optimal pH of the enzyme is neutral (close to 7), and is
considered as
GRAS (Generally Recognized/Regarded As Safe) by the FDA
(American
Food and Drug Administration).
It is because is produced by an eukaryotic organism, and the
fact of the
optimum pH close to the neutrality, what makes this strain very
important in
biotechnology and the perfect candidate to grow in cheese whey,
which is a
by-product of the cheese factories all over the world
(especially in small ones).
-
Introduction
32
The most impostant studies about an approximation to the protein
structure
of the β-galactosidase are in temporary order: a comparison with
prokaryotic
enzymes and secondary structure analysis (Poch et al. 1992),
(Athes et al. 1998)
who studied the ifluence of polyols on the structural properties
of
Kluyveromyces lactis β-galactosidase under high hydrostatic
pressure, (Becerra et
al. 1998) who find out that dimeric and tetrameric form of the
β-galactosidase
are active, and finally (Tello-Solis et al. 2005) who discover
and presented the
secondary structure of the β-galactosidase using circular
dichroism.
In the purification of the protein all began with the first
purification of
(Dickson et al. 1979).
Later the works of (Dickson et al. 1979; Becerra et al. 1998;
Becerra et al. 1998)
with different purification techniques improve the quality of
the purification.
About the production and applications in the eighties of the
past centrury,
Solomons studied the effect of lactose and β-galactosidases in
humans
(Solomons et al. 1985; Solomons et al. 1985), and Sreekrishna
constructed the
first strains capable of grow in lactose (Sreekrishna and
Dickson 1985).
In the nineties, the first prolific studies about the K. lactis
production, and the
production of ethanol from lactose arise (de Figueroa et al.
1990; Becerra et al.
1997; Kim et al. 1997; Rubio-Texeira et al. 1998).
From 2000 to present a great number of papers show the potential
of the K.
lactis β-galactosidase production and its uses to produce
ethanol from cheese
whey (Becerra et al. 2001; Becerra et al. 2001; Domingues et al.
2001; Rubio-
Texeira et al. 2001; Becerra et al. 2002; Kim et al. 2003;
Panuwatsuk and Da
Silva 2003; Ramirez Matheus and Rivas 2003; Becerra et al. 2004;
Jurascik et al.
2006; Clop et al. 2008; Guimaraes et al. 2008; Guimaraes et al.
2008; Guimaraes
et al. 2008; Ornelas et al. 2008; Guimarães et al. 2010;
Oliveira et al. 2011).
3. A. niger β-galactosidase (EC 3.2.1.3 - P29853.2)
The first research with information of its structure was
published at 1979
(Widmer and Leuba 1979), and the minimum expression of the
protein was
determined in 124.000 KDa. The pH optimum oscillate between 2.5
and 4.0.
Due to the acid optimum pH of this protein, and the fact that is
excreted
naturally by the fungus, make this protein a good candidate to
use in
biotechnology applications.
-
Introduction
33
Some of the most important studies related to the heterologous
expression of
this protein are summarized in table 1 extracted from (Oliveira
et al. 2011).
1.2. Families
On the basis of their sequence, β-galactosidases are classified
in CAZy
(Cantarel et al. 2009) within families 1, 2, 35 and 42 of
glycosyl hydrolases.
Those from eukaryotic organisms are grouped into family 35 with
the only
exceptions of K. lactis (P00723) and K. marxianus (Q6QTF4)
β-galactosidases
(99% identity), which belong to the family 2 together with the
prokaryotic β-
galactosidases from E. coli and Arthrobacter sp. Whereas the
structures of these
last two prokaryotic enzymes have been determined (Juers et al.
2000; Skalova
et al. 2005), none of the eukaryotic β-galactosidase structures
has been
reported to date. Although their sequence homology with the
prokaryotic
enzymes is significant (48% vs. E. coli and 47% vs.
Arthrobacter) there are many
differences, particularly some long insertions and deletions,
which can play an
important role in protein stability and in substrate recognition
and specificity.
1.3. Applications
β-galactosidases are used in different applications, but can be
summarized in
three most important fields:
a. Cheese whey valorisation
b. Pharmaceutical/Medical Industry
c. Other Agrofoods issues
-
Introduction Table 1
34
Source of
enzyme Expression host/plasmid
Media and culture
conditions
Extracellular recombinant β-
galactosidase activity References
A. niger Mauri distiller's yeast/pVK1.1
Modified Dw
medium+10% lactose (2-L
bioreactor)
10 U/mL (Ramakrishnan and Hartley
1993)
A. niger Brewer's yeast W204-FLO1L/ pET13.1+lacA cassette (pLD1)
SSlactose 2% (shake flasks) 17 U/mL (Domingues et al. 2000)
A. niger S. cerevisiae NCYC869-A3/pVK1.1 SSlactose 1% (Shake
Flask) 350 U/mL (Domingues et al. 2002)
A. niger S. cerevisiae NCYC869-A3/pVK1.1 SSlactose 5% (2-L
Bioreactor) 2000 U/mL (Domingues et al. 2002)
A. niger S. cerevisiae NCYC869-A3/pVK1.1 SSlactose 10% 5096 U/mL
(Domingues et al. 2002)
A. niger S. cerevisiae NCYC869-A3/pVK1.1 Cheese whey permeate
5%
lactose 2635 U/mL (Domingues et al. 2002)
A. niger S. cerevisiae NCYC869-A3/pVK1.1 SSlactose 15%+1.5%
YE
(10-L bioreactor) 7350 U/mL (Domingues et al. 2004)
A. niger S. cerevisiae NCYC869-A3/pVK1.1 SSlactose 5% (6-L
airlift
bioreactor) Maximum: 3250 U/mL (D=0.4 h−1) (Domingues et al.
2005)
A. niger S. cerevisiae NCYC869/pδ-neo+lacA cassette SSlactose 5%
(6-L airlift
bioreactor) Maximum: 2754 U/mL (D=0.1 h−1) (Oliveira et al.
2007)
K. lactis and A. niger K. lactis MW 190-9B/pSPGK1-LAC4;
pSPGK1-LAC4-LACA Culture medium is not
described (shake flasks) K. lactis/pSPGK1-LACA: up to 100 U/mL
(Rodriguez et al. 2006)
K. lactis and A. niger K. lactis MW 190-9B/pSPGK1-LAC4;
pSPGK1-LAC4-LACA Culture medium is not
described (shake flasks) K. lactis/pSPGK1-LAC4-LACA: up to
100–200 U/mL (Rodriguez et al. 2006)
-
Introduction
35
a. Cheese whey valorisation
Cheese whey is a subproduct resulted in the production of
cheese, after the
curdle separation.
It has a yellow-green colour, an elevated COD and BOD, due to
the great
quantities of nutrients it retains. Because of that it has to be
treated after the
elution to the drains.
There are two types of cheese whey:
Sweet whey: from cheeses that were produced by enzymatic
coagulation.
pH=6,5-7
Acid whey: from cheeses that were produced by acid coagulation.
pH=4,5
The composition of cheese whey is not always the same because it
depends in
the cheese master and how he/she make the cheese (the pastry
could be
washed more or less, it could have more or less salt, depends on
the type of
milk:origin, land, etc), but it could be made an average like
follows:
Lactose 50 g/L
Proteins 7,5 g/L
Lipids 5 g/L
N/NH4 0,031g/L
Lactic Acid, Vit. B, others
-
QOD 79 g/L
BOD 30.000-60.000 ppm
Dry extract 6-7%
Salts 8-10% Dry extract
Phosphates 0,39 g/L
There are a high variety of cheese whey uses: SCP (Single Cell
Protein),
biosurfactants production, biopolymers production, lactic acid
production,
bacteriocines production, biogas production, production of
recombinant
proteins, GOS and bioethanol production
b. Pharmaceutical/Medical Industry
On one handfor pharmaceutical industry, interest in
β-galactosidases is due to
the reproducibility of their activities which are used in a lot
of commercial kits
-
Introduction
36
as a reporter gene and so to reduce the problems associated to
the lactose
intolerance (Solomons et al. 1985; Solomons et al. 1985; Vesa et
al. 2000;
Bhatnagar and Aggarwal 2007; Ibrahim et al. 2009; O'Connell and
Walsh
2009).
On the other hand, the medical industry interest is due lactose
intolerance in
humans affects to over 70% of the world's adult population
(Oliveira et al.
2011), which typical symptoms are abdominal pain, gas, nausea
and diarrhea.
c. Other Agrofoods issues
In the food industry β-galactosidase is also used to enhance the
sweetness of
lactose for example in desserts like ice-creams, cakes, etc.
2. Protein Structure Analysis
Determine the 3D protein structure is essential because the
function of a
biological macromolecule is related to its 3D shape, so
knowledge about the
structure is essential to understand the function.
The 3D protein structure benefits:
1. Understanding biological processes at the atomic level
2. Study interactions among proteins and/or other molecules
3. Design of specific inhibitors/activators for a protein – drug
discovery
The three most important strategies to determine the protein
structure are:
Nuclear Magnetic Resonance (NMR), X-Ray Crystallization (XRC)
and cryo-
Electron Microscopy (EM).
NMR is based in the concept that NMR active nuclei absorb
electromagnetic
radiation at a frequency characteristic of the isotope. The
resonant frequency,
energy of the absorption and the intensity of the signal are
proportional to the
strength of the magnetic field.
The advantages of NMR over XRC are:
-
Introduction
37
1. Can be performed in the solution-state, so the structures may
be more
physiologically relevant.
2. Some proteins do not give diffraction-quality crystals.
3. Provides dynamics and other information: internal mobility,
flexibility,
order-disorder, hydrogen exchange rates, pKa values, binding
constants, conformational exchange rates, …
Disadvantages of NMR over XRC are:
1. Molecular weight limitations: 50 kDa for complete
structure
determination and 100 kDa for local or partial analysis.
2. Stable-isotope enrichment usually required: need efficient
bacterial
expression system.
3. Structure determination methods more time consuming,
difficult …
XRC is a method of determining the arrangement of atoms within a
crystal, in
which a beam of X-rays strikes a crystal and diffracts into many
specific
directions. The crystal is an ordered solid in where a basic
organizational unit
is repeated (Lesk 2001).
From the angles and intensities of these diffracted beams, a
crystallographer
can produce a three-dimensional picture of the density of
electrons within the
crystal. From this electron density, the mean positions of the
atoms in the
crystal can be determined, as well as their chemical bonds,
their disorder and
various other information.
In an X-ray diffraction measurement, a crystal is mounted on a
goniometer
and gradually rotated while being bombarded with X-rays,
producing a
diffraction pattern of regularly spaced spots known as
reflections. The two-
dimensional images taken at different rotations are converted
into a three-
dimensional model of the density of electrons within the crystal
using the
mathematical method of Fourier transforms, combined with
chemical data
known for the sample.
EM is the most innovative of the fourth methods, and allows the
structure
determination of macromolecules and biological aggregates at
molecular
resolution (7 to 30 Å) up to near atomic resolution (2-3 Å) that
have not been
stained or fixed in any way, showing them in their native
environment.
-
Introduction
38
The advantages over XRC and NMR are:
1. There is no limit to the size of the structure in study, so
large and
complex structures which cannot be studied with the
mentioned
strategies can be solved with EM (for ex. membrane
proteins).
2. Relatively small sample amounts
3. Cryo methods allow the observation of the molecules in their
aqueous
native state, close to physiological conditions.
3. Directed Evolution
Engineering the specificity and properties of enzymes and
proteins within
rapid time frames has become feasible with the advent of
directed evolution.
In the absence of detailed structural and mechanistic
information, new
functions can be engineered by introducing and recombining
mutations,
followed by subsequent testing of each variant for the desired
new function. A
range of methods are available for mutagenesis, and these can be
used to
introduce mutations at single sites, targeted regions within a
gene or randomly
throughout the entire gene. In addition, a number of different
methods are
available to allow recombination of point mutations or blocks of
sequence
space with little or no homology. Currently, enzyme engineers
are still learning
which combinations of selection methods and techniques for
mutagenesis and
DNA recombination are most efficient. Moreover, deciding where
to
introduce mutations or where to allow recombination is actively
being
investigated by combining experimental and computational
methods. These
techniques are already being successfully used for the creation
of novel
proteins for biocatalysis and the life sciences (Williams et al.
2004).
Principally, the directed evolution methods are divided in
recombinative and
non-recombinative methods.
Non-recombinative methods generally create diversity via point
mutation and
include the directed substitution of single amino acids, the
insertion or
deletion of more than one amino acid, for example by cassette
mutagenesis,
and random mutagenesis across the whole gene.
The simplest, and still a popular, method of choice for
introducing diversity is
Error Prone – PCR (EP-PCR). The mutation rate can be adjusted so
that,
usually, an average of 1–2 amino acid mutations is introduced
per gene
product (Moore et al. 1997).
-
Introduction
39
It is generally accepted that using a low mutation rate
increases the probability
of discovering beneficial mutations, since most random mutations
are either
neutral or deleterious.
EP-PCR at low mutation rate suffers from one drawback: there is
an inherent
bias introduced since on average only 5.6 amino acids per codon
can be
accessed given the substitution of a single nucleotide. In
addition, the inherent
bias of polymerases further reduces the diversity that can be
accessed by EP-
PCR.
These problems can be overcome by the use of gene site
saturation
mutagenesis (GSSM).
GSSM is a method that uses sets of degenerate primers to
introduce all 19
amino acid substitutions at every position of the gene to
produce every
possible single amino acid mutant (DeSantis et al. 2003).
In the case of recombinative methods DNA shuffling is still the
most popular
method of recombining DNA, whether homologous genes from
different
sources are being recombined, or for the recombination of point
mutations.
Briefly, the original DNA shuffling technique (Stemmer 1994;
Stemmer
1994)involves the controlled fragmentation of the source DNA
using DNase
I, followed by a primer-less, reassembly PCR reaction, which
gradually
produces full-length recombined sequences. Finally, the small
amount of
fulllength gene present in the reassembly reaction is amplified
by a standard
PCR reaction in the presence of flanking primers.
Other recombinative method is the Staggered Extension Process
(StEP)
which uses a simple PCR reaction with very short elongation
times;
recombination occurs where partially elongated strands melt and
anneal to a
new template, producing a crossover (Zhao et al. 1998).
One more recombinative method is Random Chimeragenesis on
Transient
Templates (RACHITT), which is similar to DNA shuffling but
requires many
more experimental steps; however the method does produce a much
larger
number of crossovers than basic DNA shuffling (Coco et al.
2001).
Two other also known recombinative methods are Synthetic
Shuffling and
Assembly of Designed Oligonucleotides (ADO): They use of
entirely
synthetic oligonucleotides that result in the production of
fulllength genes
with defined crossover points and composition. Both techniques
result in
-
Introduction
40
significantly increased recombination frequency and have been
validated
experimentally (Zha et al. 2003).
There are two last recombinative methods, the first is the
Incremental
Truncation for the Creation of HYbrid enzymes (ITCHY) which
involves the
direct ligation of truncated N- and C-terminal fragments of two
genes,
removing the requirement of homology (Ostermeier et al. 1999).
Crossovers
occur at random positions, and the initial products only contain
a single
crossover. DNA shuffling of the ITCHY products can generate
products with
multiple crossovers, and plasmid systems are available for
selection of only in
frame ligation products.
The second is a computational algorithm (SCHEMA) which
identifies optimal
crossover points using structural information to identify sites
with minimal
interaction with the rest of the protein (Voigt et al.
2002).
Once the directed evolution method was chosen and applied, the
following
step is to decide the optimum screening strategy, which is the
most critical
step in the directed evolution, and usually is a bottleneck.
There are different screening strategies, but the most common
ones are
summarized in this review (Arnold F H et al. 2003).
As reviewed, the directed evolution methods make to evolve
easily and fastly
proteins (principally enzymes), which is really important in
biotechnology to
solve problems like the cheese whey.
Different techniques have been applied to develop strains
capable of secrete a
directed evolution β-galactosidase, that makes the strain able
to grow in
cheese whey and consume the nutrients which make it toxic to
environment.
1 Fowler et. al think initially that the E.coli β-galactosidase
was composed of 1171 residues and has a molecular weight of 135
KDa, but future experiments demonstrate that it is composed of 1024
amino acids and its molecular weight is 116KDa.
2 In the chapter 3of this thesis, the three-dimensional
structure is presented and discussed.
-
41
Objectives
-
Objectives
43
The aim of this thesis is to determine and analyze the structure
of the
Kluyveromyces lactis β-galactosidase and express and apply
directed evolution
techniques in β-galactosidases with high biotechnological
potential, and
specifically:
1. Expression, purification and crystallization of the
Kluyveromyces lactis β-
galactosidase.
2. Structural characterization ot the Kluyveromyces lactis
β-galactosidase.
3. Development of a hybrid Kluyveromyces lactis-Aspergillus
niger β-
galactosidase.
a. Construction, and biochemical analysis of the hybrid
enzyme
b. Production of the hybrid enzyme in a continuous culture
4. Development of a Saccharomyces cerevisiae strain which
expresses the
Aspergillus niger β-galactosidase and application of directed
evolution
methods to modify the optimum pH of the enzyme.
-
45
Outline of this thesis
-
Outline of this thesis
47
β-galactosidases are probably one of the most important proteins
in terms of
biotechnology potential due to their applications in Pharmacy,
Medicine,
Agrofood Industries, etc.
Within the group of -galactosidases, the most interesting ones,
due to its
biological and chemical properties are Escherichia coli,
Kluyveromyces lactis and
Aspergillus niger -galactosidases.
The first one, the Escherichia coli -galactosidase, has been
very thoroughly
studied, so this thesis deals with the study of the other two
-galactosidases,
focusing more in the K. lactis-galactosidase due to its use in
sweet cheese
whey which is the most abundant in Galicia.
Chapter 1 presents the details of a full-factorial design used
to find conditions
for growing good-quality crystals of K. lactis β-galactosidase.
The application
of a full-factorial approach to protein crystallization could
reduce substantially
the number of crystallization trials. The method is based on a
factorial
approach to experimental design, permits the assay of a large
number of
crystallization conditions with as few experiments as possible.
This is
accomplished by varying more than one factor at a time in a
given experiment;
this saves material, and from the analysis of the results, it is
possible to readily
determine the factors that are critical for crystallization.
Chapter 2 is about the expression, purification, optimization of
crystallization,
and diffraction of crystals to obtain the K. lactis
β-galactosidase structure. This
protein has been expressed and purified in yeast for the
crystallization trials.
However, even optimization of the best crystallization
conditions yielded
crystals with poor diffraction quality that precluded further
structural studies.
Finally, thanks to the streak seeding technique, the crystal
quality was
improved and a complete diffraction data set was collected at
2.8 Å resolution.
Chapter 3 describes X-ray crystallographic studies and an
analysis of K. lactis
β-galactosidase. β-galactosidase sequences can be deduced from
various
databases, and these can be classified into four different
glycoside hydrolase
(GH) families 1, 2, 35, and 42, based on functional
similarities. Those from
eukaryotic organisms are grouped into family 35 with the only
exceptions of
K. lactis and K. marxianus β-galactosidases (99% identity),
which belong to the
family 2 together with the prokaryotic β-galactosidases from
Escherichia coli and
Arthrobacter sp. Whereas the structures of these last two
prokaryotic enzymes
have been determined, none of the eukaryotic β-galactosidase
structures has
-
Outline of this thesis
48
been reported. In fact, to date, the X-ray crystal structures of
eight different
microbial β-galactosidases are available in the PDB, although
none of the
enzymes with solved structures is known to be used in food
processing. Here
it is reported the three-dimensional structure at 2.75 Å
resolution and the
complex structure with galactose at 2.8 Å resolution of the
β-galactosidase
from K. lactis, one of the most important and widely used
enzymes of the food
industry.
Chapter 4 shows the construction and analysis of a two hybrid
proteins from
the β-galactosidase of K. lactis, intracellular, and its A.
niger homologue that is
extracellular; and the production of the hybrid K. lactis-A.
niger β-galactosidase
in a continuous immobilized culture. A hybrid protein between K.
lactis and
A. niger β-galactosidases was constructed that increases the
yield of the protein
released to the growth medium. Modifications introduced in the
construction,
besides to improve secretion, conferred to the protein
biochemical
characteristics of biotechnological interest. This hybrid
β-galactosidase
showed an optimal pH around the neutrality and it had a better
stability at
high temperatures compared with the wild protein from yeast.
Different
culture mediums were assayed to scale-up the production of this
hybrid β-
galactosidase. The use of spent grains (a by-product of brewery
industry) as an
immobilizing material (in which the yeast can grow inside) to
stabilize the
production of the protein, was checked too.
Chapter 5 presents the construction, expression and analysis of
three new
recombinant Saccharomyces cerevisiae strains expressing A. niger
β-galactosidase,
and the directed evolution of the enzyme to modify its optimum
pH. The
development of S. cerevisiae strains with the capability of
metabolizing lactose
is an important biotechnological objective, and here it is
described the
construction of new recombinant S. cerevisiae strains which was
able to express
and secrete the extracellular and thermostable β-galactosidase
from A. niger up
to 90% of the total β-galactosidase activity into the growth
medium. Finally
using a directed evolution technique, mutations in the enzyme
were done, in
order to modify its optimum pH.
-
49
CHAPTER 1
Kluyveromyces lactis β-galactosidase
crystallization using full-factorial experimental design
Ángel Pereira Rodríguez, Rafael Fernández Leiro, M. Esperanza
Cerdán, M. Isabel González Siso, Manuel Becerra Fernández
Journal of Molecular Catalysis B: Enzymatic 52–53 (2008)
178–182
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Kluyveromyces lactis β-galactosidase crystallization using
full-factorial experimental design
51
SUMMARY
Kluyveromyces lactis β-galactosidase is an enzyme with
numerous
applications in the environmental, food and biotechnological
industries.
Despite of its biotechnological interest, its three-dimensional
structure has not
yet been determined. The growth of suitable crystals is an
essential step in the
structure determination of a protein by X-ray crystallography.
At present,
crystals are mostly grown using trial-and-error procedures since
their growth
often depends on the combination of many different factors.
Testing the
influence on crystallization of even only a small number of
these factors
requires many experimental set-ups and large amounts of protein.
In the
present work, a full-factorial design has been used in order to
find conditions
for obtaining good-quality crystals of K. lactis
β-galactosidase. With this full-
factorial method protein crystals have been obtained.
INTRODUCTION
The microbial lactase or β-galactosidase (β-D-galactoside
galactohydrolase, EC 3.2.1.23) from the yeast Kluyveromyces
lactis, the
enzyme which is responsible for the hydrolysis of lactose into
glucose and
galactose, has outstanding biotechnological interest. Therefore
it has attracted
the attention of researchers and industries because of its
important
applications in the fields of medicine (treatment of lactose
intolerance), food
technology (to prevent lactose crystallization and increase its
sweetening
power) and the environment (cheese whey utilization). Although
much of the
work on K. lactis β-galactosidase has dealt with the production
(Becerra et al.
1997; Becerra et al. 2001; Becerra et al. 2002; Becerra et al.
2004), the use
(Becerra et al. 2003) and biochemical characterization (Athes et
al. 1998;
Becerra et al. 1998; Tello-Solis et al. 2005), to the best of
our knowledge, very
little has been reported about its structure (Tello-Solis et al.
2005).
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Chapter 1
52
The growth of suitable protein crystals is an essential step in
the
structure determination of a protein by X-ray crystallography.
At present,
crystals are mostly grown using trial-and-error procedures,
whereby various
factors: pH, temperature, salt concentration, etc, are
systematically varied until
crystals are obtained. Usually, in these experiments, the
different factors are
varied only over a narrow range of values. The use of this
method often
requires large amounts of material and is frequently time
consuming.
Carter and Carter (Tello-Solis et al. 2005) demonstrated that
the
application of a full-factorial approach to protein
crystallization could reduce
substantially the number of crystallization trials. Their
method, which is based
on a factorial approach to experimental design (Fisher 1942),
permits the assay
of a large number of crystallization conditions with as few
experiments as
possible. This is accomplished by varying more than one factor
at a time in a
given experiment; this saves material, and from the analysis of
the results, it is
possible to readily determine the factors that are critical for
crystallization. We
present here the details of a full-factorial design to find
conditions for growing
good-quality crystals of K. lactis β-galactosidase.
MATERIAL AND METHODS
Strains and culture conditions
The following strains were used: Kluyveromyces lactis
NRRL-Y1140
(MATa, wild type) and Saccharomyces cerevisiae BJ3505
(pep4::HIS3, prb-Δ1.6R
HIS3, lys2-208, trp1-Δ101, ura 3-52, gal2, can1). The BJ3505
strain was
purchased from Eastman Kodak.
Liquid batch cultures of wild type and transformed cells were
grown in
Erlenmeyer flasks filled with 20% volume of culture medium at
250 rpm and
30 ºC, unless otherwise stated. K. lactis wild type cells were
growth in YPL (1%
yeast extract, 0.5% bactopeptone, 0.5% lactose) whereas
transformed S.
-
Kluyveromyces lactis β-galactosidase crystallization using
full-factorial experimental design
53
cerevisiae BJ3505 cells were growth in YPHSM (1% yeast extract,
8%
bactopetone, 1% dextrose, 3% glycerol, 20mM CaCl2). In the late
case, as
inocula, a suitable volume of a stationary phase culture in
complete medium
(CM) (Lowry et al. 1983) without the corresponding auxotrophic
amino acid
was added to obtain an initial OD600 of 0.2. Samples were taken
at regular
time intervals to measure growth (OD600) and intracellular
β-galactosidase
activity.
Vectors
The YEpFLAG1-LAC4 (Becerra et al. 2001) containing the LAC4
gene, which codes for K. lactis β-galactosidase, inserted
between the yeast
ADH2 promoter and CYC1 terminator was used. This plasmid also
contains
the sequence of the FLAG peptide for the immunological detection
and
affinity purification of the FLAG fusion protein.
Molecular biology procedures
Yeast strains were transformed using the lithium acetate
procedure (Ito
et al. 1983). Plasmid uptake and β-galactosidase production by
the transformed
strains were identified on plates with the chromogenic substrate
X-gal in the
corresponding auxotrophic medium.
β-galactosidase activity assays and protein determinations
The method of Guarente (Ito et al. 1983) as previously
described
(Becerra et al. 2001) was used. One enzyme unit (E. U) was
defined as the
quantity of enzyme that catalyzes the liberation of 1 μmol of
ortho-
nitrophenol from ortho-nitrophenyl-β-D-galactopyranoside per min
under
assay conditions.
Protein was determined by the method of Bradford (Bradford
1976)
using bovine serum albumin (Sigma) as a standard.
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Chapter 1
54
Preparation of crude protein extracts
Crude protein extract was prepared as described previously
(Becerra et
al. 1998) from cells cultured in 1 litre of YPL or YPHSM up to
an A600nm of
2 (about 3 mg dry wt/ml).
Purification of β-galactosidase
The purification of β-galactosidase from a crude protein extract
of the
strain of K. lactis NRRLY1140 and from a YEpFLAG1-LAC4
transformed S.
cerevisiae BJ3505 strain was performed using different
chromatographical
techniques.
In the first trial, a column with 5 ml
agarose-p-aminopheyl-β-D-
thiogalactoside (Sigma Chemical, USA) was equilibrated with 50
mM
phosphate buffer pH 7, and the enzyme was eluted with 0.1 M
sodium borate,
pH 10. Fractions of 1 ml were collected at a flow rate of 100
μl/min. The pH
of the collected fractions was neutralized to avoid
denaturation.
In the second purification method assayed, a column with 0.2 ml
of
ANTI-FLAG M2 affinity gel (Sigma Chemical, USA), useful for
purification
of FLAG fusion proteins, was equilibrated with TBS (150 mM NaCl,
50 mM
Tris-HCl pH 7.4) and the elution of the bound FLAG fusion
protein was by
competition with a solution containing 100 μg/ml FLAG peptide
(Sigma
Chemical, USA).
All purification steps were carried out at 4ºC. The
β-galactosidase
activity was assayed in the eluted fractions obtained from
chromatographic
steps. Active fractions were pooled and, when required,
concentrated by
filtration in Amicon ULTRA-4 (Millipore, UFC 803024).
Polyacrylamide gel electrophoresis
This was performed as described in Becerra (Becerra et al.
1997).
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Kluyveromyces lactis β-galactosidase crystallization using
full-factorial experimental design
55
Protein crystallization
Crystals were grown at several conditions at 20ºC by vapour
diffusion
in hanging drops containing 1-3 μl of protein solution (9 mg/ml)
and 1 μl of
reservoir solution.
Experimental design and statistical data analysis
Factorial experimental design was created and data were analyzed
with
the aid of version 5.1 of the STATGRAPHICS Plus software for
Windows
(Statistical Graphics Corporation).
The statistical significance of differences between means
was
determined by Student´s t-test performed with the same software;
p values
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Chapter 1
56
for the same enzyme (Becerra et al. 1998). The results of this
purification
process are summarized in Table 1.
Table 1: Summary of the purification procedure of K. lactis
β-galactosidase by affinity
chromatography (A) and of K. lactis β-galactosidase fused to the
FLAG peptide by immunoaffinity
(B).
Step Total protein
(mg)
Total E.U. Yield (%) Specific
activity (E.
U/mg)
Purification
factor
A Crude extract 87 137 250 100 1 577.59 1
Affinity and
microultrafiltration
1.79 23 882 17.4 4 259.5 2.7
B Crude extract 133.10 104 610 100 785.95 1
Immunoaffinity
chromatography
1.91 71 980 68.81 37 705.61 47.98
K. lactis β-galactosidase was also purified from a S. cerevisiae
BJ3505
strain transformed with YEpFLAG1-LAC4 by affinity purification
of the
FLAG fusion protein. In this case, a 1.43% protein recovery with
a yield of
68.8% and an increase in specific activity of 47.98-fold was
obtained (Table 1).
The homogeneity of the isolated β-galactosidases was examined
by
SDS-PAGE of the purified enzyme (Figure 1), both preparations
show a main
protein band with the approximate molecular weight of 124 kDa
that agreed
with the one predicted from the sequence of LAC4, the unique
gene coding
for β-galactosidase present in the K. lactis genome (Poch et al.
1992).
These data demonstrate the usefulness of both tested
purification
procedures for obtaining K.lactis β-galactosidase protein.
Although the
second procedure gave the highest purification factor and turned
out to be
more effective.
-
Kluyveromyces lactis β-galactosidase crystallization using
full-factorial experimental design
57
Fig. 1. SDS-PAGE of purified K. lactis β-galactosidase. Lane 1,
molecular weight markers; lane 2, 60 µg of a crude extract from K.
lactis NRRL-Y1140; lane 3, 1.5 µg of K. lactis β-galactosidase
purified by affinity chromatography from K. lactis NRRL-Y1140, lane
4, 90 µg of a crude extract of S. cerevisiae BJ3505 transformed
with YEpFLAG1-LAC4; lane 5, 3.5 µg of K. lactis β-galactosidase
purified by immunoaffinity. β-Galactosidase is indicated by an
arrow.
Initial crystallization screens
Crystallization of macromolecules is usually performed by a
somewhat
organized trial-and-error procedure using available kits.
However, use of these
kits locks the experimenter to a relatively narrow set of
historical conditions.
In our case, the search strategy to get optimal crystallization
of K. lactis β-
galactosidase was based on the use of conditions that have
already rendered
useful for obtaining crystals of a homologous protein with
similar size and
function, the β-galactosidase from E. coli (Juers et al. 2003).
Reproducing these
conditions, small protein crystals were obtained in presence of
0.1 M Tris pH
8.0 and different concentrations of (NH4)2SO4 (0.02 and 0.2 M)
and PEG
6000 (5% and 10 %) (Figure 2).
1 2 3 4 5 212
121
100
54.4
kDa
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Chapter 1
58
Fig. 2. Photography of one K. lactis _-galactosidase crystal
obtained in presence of 0.1M Tris, pH 8.0, 0.02M (NH4)2SO4 and 5%
PEG 6000 (A). Protein crystals obtained using the full-factorial
design approach (B and C). Photographs were taken with the
objective 40×.
Optimization of the crystallization conditions
The formation of crystals depends on the concentration of
macromolecule and precipitant. At higher concentrations of
macromolecule,
less precipitant is required for crystallization. The function
of the different
precipitants such as polyethylene glycol and ammonium sulphate
in the
crystallization drop is to alter the protein-solvent or
protein-protein contacts
so that the protein molecules precipitate out of solution,
preferably as ordered
crystals and not as disordered aggregates. In our case, in order
to identify
optimal conditions for crystal growth, including crystal volume
and shape
improvements, we studied, by means of a full-factorial design,
the influence of
three variables (% PEG 6000, (NH4)2SO4 and protein
concentrations), and
their interactions on the response. The range and coding
criteria of the
variables used are given in Table 2 and Table 3 shows the
experimental matrix
and the results obtained for the analysed response, the quality
of crystals. The
quality obtained was quantified taking into account three
parameters:
morphology, size and amount of crystals. A score of 2 points was
given to
crystals with regular morphology and 1 point to crystals with
irregular
morphology. In the center of the experimental domain (0 coded
value to the
three variables), crystals showed a similar size, and therefore
were considered
-
Kluyveromyces lactis β-galactosidase crystallization using
full-factorial experimental design
59
as average size and scored with 2 points. Bigger crystals than
those obtained in
the center of domain were scored with 3 points, and smaller
crystals with 1
point. Finally, 1 point was given to conditions which showed
multiple crystals,
and two points were given to conditions with few crystals.
Table 2 Experimental domain and codification of the variables
used in the full-factorial design
Natural Values
Coded values PEG 6000
(P: %)
(NH4)2SO4
(A: M)
Protein concentration
-1 5 0.02 10
0 10 0.1 15
+1 15 0.18 20
Codification: Vc = (Vn −V0)/DVn; decodification: Vn = V0 + (ΔVn
×Vc) where Vc is the coded value, Vn is the natural value, V0 is
the natural value in thecenter of the experimental domain and ΔVn
is the increase in the natural valuecorresponding to 1U of growth
in the coded value.
Table 3: Experimental results of the full-factorial design (23)
for the study of K. lactis β-galactosidase crystals quality
obtained taking into account three parameters: morphology, size and
amount of crystals. Variables according to Table 2.
P A PRO Quality of Crystals
1 1 1 1 6
2 -1 1 1 4
3 1 1 -1 0
4 -1 1 -1 5
5 1 -1 1 5
6 -1 -1 1 5
7 1 -1 -1 4
8 -1 -1 -1 0
9 0 0 0 5
10 0 0 0 4
11 0 0 0 4
12 0 0 0 4
Variables are according to Table 2.
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Chapter 1
60
The ANOVA table (Table 4A) divides the variability in the
response
into separate pieces for each of the effects. It then tests the
statistical
significance of each effect by comparing the mean square against
an estimate
of the experimental error. In this case, 3 effects have P-values
less than 0.05
(with asterisk in Table 4A), indicating that they are
significantly different from
zero at the 95.0% confidence level.
After removing the no significant coefficients, the P-value for
lack-of-
fit in the ANOVA table (Table 4B) is greater to 0.05 (0.1149)
and the model
appears to be adequate for the observed data at the 95.0%
confidence level.
The lack of fit test is designed to determine whether the
selected model is
adequate to describe the observed data, or whether a more
complicated model
should be used. The test is performed by comparing the
variability of the
current model residuals to the variability between observations
at replicate
settings of the factors.
The R-Squared statistic indicates that the model, as fitted,
explains
91.7017% of the variability in the response. The adjusted
R-squared statistic,
which is more suitable for comparing models with different
numbers of
independent variables, is 88.5898 %. The standard error of the
estimate shows
the standard deviation of the residuals to be 0.64145. The mean
absolute error
(MAE) of 0.423611 is the average value of the residuals.
The Durbin-Watson (DW) statistic tests the residuals to
determine if
there is any significant correlation based on the order in which
they occur in
the data file. Since the DW value is greater than 1.4 (2.05063),
there is
probably not any serious autocorrelation in the residuals.
The system can be represented by the following codified
equation
(significance tested by Fisher F-test) in which only the
concentration of
protein, the interaction between the % PEG 6000 and ammonium
sulphate
-
Kluyveromyces lactis β-galactosidase crystallization using
full-factorial experimental design
61
concentration and the interaction between the three variables
present
influence in the response:
Quality of crystals = 3.83333 + 1.375xPRO - 0.875 x P x A +
1.375
x P x A x PRO
Table 4: Analysis of variance for the response (quality of the
crystals) in the full-factorial design
(23) studied before (A) and after (B) removing the no
significant coefficients and analysis of the
significance and adequacy of the proposed model
Source
Sum of
Squares
Degree of
Freedom Mean Square F-Ratio P-Value
A P:PEG 0.125 1 0.125 0.50 0.5305
A:AMMONIUM 0.125 1 0.125 0.50 0.5305
PRO:PROTEIN 15.125 1 15.1250 60.50 0.0044*
PxA 6.125 1 6.125 24.50 0.0158*
PxPRO 1.1250 1 1.125 4.50 0.1240
AxPRO 0.125 1 0.125 0.50 0.5305
PxAxPRO 15.1250 1 15.1250 60.50 0.0044*
Lack-of-fit 1.04167 1 1.04167 4.17 0.1339
Pure error 0.75 3 0.25
B PRO:PROTEIN 15.1250 1 15.1250 47.06 0.0002
PxA 6.1250 1 6.1250 19.06 0.0033
PxAxPRO 15.1250 1 15.1250 47.06 0.0002
Lack-of-fit 1.04167 1 1.04167 3.24 0.1149
Pure Error 2.25 7 0.321429
Total (Corr.) 39.6667 11
(*) Significant coefficients. Variables according to Table 2. R2
= 91.7017%; R2 (adjusted for d.f.) = 88.5898%; standard error of
est. = 0.64145; mean absolute error = 0.423611; Durbin–Watson
statistic = 2.05063 (P = 0.4610).
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Chapter 1
62
Fig. 3. Response surfaces obtained to identify optimal
conditions for K. lactis β-galactosidase crystal growth according
to the experimental plan defined in Table 2. Crystals score =
response (quality of crystals taking into account: morphology, size
and amount of crystals). Variable values and nomenclature can be
seen in Table 2.
PEG = 0, 0
AMMONIUMPROTEINC
RIS
TA
LS
SC
OR
E
-1 -0.5 0 0.5 1-1
-0.50
0.51
0123456
AMMONIUM = 0, 0
PEGPROTEIN
CR
IST
AL
S S
CO
RE
-1 -0.5 0 0.5 1-1
-0.50
0.51
0123456
PROTEIN = 0, 0
PEGAMMONIUM
CR
IST
AL
S S
CO
RE
-1 -0.5 0 0.5 1-1
-0.50
0.51
0123456
PEG = 0, 0
AMMONIUMPROTEINC
RIS
TA
LS
SC
OR
E
-1 -0.5 0 0.5 1-1
-0.50
0.51
0123456
PEG = 0, 0
AMMONIUMPROTEINC
RIS
TA
LS
SC
OR
E
-1 -0.5 0 0.5 1-1
-0.50
0.51
0123456
AMMONIUM = 0, 0
PEGPROTEIN
CR
IST
AL
S S
CO
RE
-1 -0.5 0 0.5 1-1
-0.50
0.51
0123456
AMMONIUM = 0, 0
PEGPROTEIN
CR
IST
AL
S S
CO
RE
-1 -0.5 0 0.5 1-1
-0.50
0.51
0123456
PROTEIN = 0, 0
PEGAMMONIUM
CR
IST
AL
S S
CO
RE
-1 -0.5 0 0.5 1-1
-0.50
0.51
0123456
PROTEIN = 0, 0
PEGAMMONIUM
CR
IST
AL
S S
CO
RE
-1 -0.5 0 0.5 1-1
-0.50
0.51
0123456
-
Kluyveromyces lactis β-galactosidase crystallization using
full-factorial experimental design
63
Some of the more representative surface responses corresponding
to
the mentioned equation are represented in Figure 3. These
surfaces are planes
defined by pairs of variables having the third variable fixed
(values -1, +1). As
can be seen in Figure 3, the response increases in each
situation of our
experimental domain when the concentration of the protein is
increased
(positive coefficient). The effect of % PEG 6000 and ammonium
sulphate
concentration, for the same protein concentration, is more
complex because
response increases in the corners, high % PEG 6000 and small
ammonium
sulphate concentration and vice versa small % PEG 6000 and
high
ammonium sulphate concentration. Therefore, the highest values
of the
response are obtained in the corners: P=+1, A=-1, PRO=+1 or
P=-1, A=+1,
PRO=+1.
Some of the K. lactis β-galactosidase crystals obtained with the
optimal
conditions obtained by this approach are shown in Figure 2.
CONCLUSIONS
A methodical and efficient approach has been carried out to
growth K.
lactis β-galactosidase crystals. The full-factorial screen with
response surface
optimization allowed us to find conditions for growing good
quality crystals
with a small number of experiments to be performed. Optimal
crystallization
conditions for 20 μg of K. lactis β-galactosidase were obtained
in the presence
of 0.1 M Tris-HCl, pH 8, 15% PEG 6000 and 0.02 M (NH4)2SO4.
Advantages
obtained in this approach include improvements in
β-galactosidase crystal
volume and shape and also in reproducibility. Similar designs
could be of
interest to get crystals from other proteins which have special
difficulties to
solve.
-
Chapter 1
64
Acknowledgements
This research was supported by PGIDIT03BTF10302PR grant from
the Xunta de Galicia (Spain). A.P.R. was initially supported by
the
PGIDT01PX110303PN grant from Xunta de Galicia (Spain) and later
by a
fellowship from the Universidade da Coruña (Spain), R.F.L.
received a
fellowship from the Universidade da Coruña (Spain) and later was
also the
recipient of a FPU fellowship from MECD. Part of the results
presented here
has been communicated at the 8th International Symposium on
Biocatalysis
and Biotransformations Biotrans 2007 (Oviedo, 2007).
-
65
CHAPTER 2
Crystallization and preliminary X-ray
crystallographic analysis of β-
galactosidase from Kluyveromyces
lactis
Ángel Pereira-Rodríguez, Rafael Fernández-Leiro, M. Isabel
González Siso, M. Esperanza Cerdán, Manuel Becerra and
Julia Sanz-Aparicio
Acta Crystallographica Section F Structural Biology and
Crystallization
Communications (2010) 66: 297-300
-
Crystallization and preliminary X-ray crystallographic analysis
of β-galactosidase from Kluyveromyces lactis
67
SUMMARY
β-galactosidase from Kluyveromyces lactis catalyses the
hydrolysis of the β-
galactosidic linkage in lactose. Due to much industrial
applications the
biotechnological potential of this enzyme is substantial. This
protein has been
expressed and purified in yeast for the crystallization trials.
However, even
optimization of the best crystallization conditions yielded
crystals with poor
diffraction quality that precluded further structural studies.
Finally, thanks to
the streak seeding technique, the crystal quality was improved
and a complete
diffraction data set was collected at 2.8 Å resolution.
INTRODUCTION
The enzyme β-galactosidase (β-D-galactoside galactohydrolase,
EC
3.2.1.23) catalyzes the hydrolysis of the disaccharide lactose
into glucose and
galactose. Enzymes with this activity are present in
microorganisms, plants
and animals, and have multiple biotechnological applications.
β-galactosidases
are useful in the treatment of lactose intolerance (Bhatnagar
and Aggarwal
2007) and they are frequently used in the food industry in order
to increase
the sweetening power of the natural saccharides (Gonzalez Siso
1996). In
addition, they are also employed in a treatment and
transformation of cheeses
whey (Gonzalez Siso 1996). β-galactosidase from the yeast
Kluyveromyces lactis
(Kl-β-Gal) is one of the most frequently used β-galactosidases
in the
biotechnological industry due to its favourable biochemical
properties: an
optimal neutral pH, and a higher stability than found for
β-galactosidases
from the other sources (i.e. fungal galactosidases).
Furthermore, K. lactis is a
GRAS (Generally Recognized as Safe) organism by the American
Food and
Drug Administration.
Kl-β-Gal (P00723) is encoded by the gene LAC4 (Gene ID:
2897170).
Although the gene was sequenced in 1992 (Poch et al. 1992), the
structure of
-
Chapter 2
68
the protein has not yet been reported. The molecular weight of
the Kl-β-Gal
monomer is 119 kDa and it was shown that only the dimeric and
tetrameric
forms of the protein are active, with the tetramer being more
active than the
dimer (Becerra et al. 1998).
On the basis of their sequence, β-galactosidases are classified
in CAZy
(Cantarel et al. 2009) within families 1, 2, 35 and 42 of
glycosyl hydrolases.
Those from eukaryotic organisms are grouped into family 35 with
the only
exceptions of K. lactis and K. marxianus β-galactosidases (99%
identitical),
which belong to the family 2 together with the prokaryotic
β-galactosidases
from Escherichia coli and Arthrobacter sp. Whereas the
structures of these last
two prokaryotic enzymes have been determined (Juers et al. 2000;
Skálová et al.
2005), none of the yeast β-galactosidases structures has been
reported to date.
Although their sequence similarity with the prokaryotic enzymes
is significant
(48% vs. E. coli and 47% vs. Arthrobacter) there are many
differences,
particularly some long insertions and deletions, which might
play an important
role in protein stability and in substrate recognition and
specificity.
Knowledge of the Kl-β-Gal three-dimensional structure will
provide an
important insight into understanding of the mechanisms of
catalysis and
should lead to improvements of its biotechnological applications
by rational
protein engineering. In this study, we describe expression,
purification and
preliminary X-ray crystallographic studies of Kl-β-Gal.
MATERIAL AND METHODS
Expression and purification
The gene LAC4 (Gene ID: 2897170) was amplified by PCR from
the
pLX8 plasmid, and cloned in the YEpFLAG vector (Eastman Kodak
Company)
as previously reported (Becerra et al., 2001) The construct was
used to
transform S. cerevisiae BJ3505 cells (Eastman Kodak Company) by
the procedure
-
Kluyveromyces lactis β-galactosidase crystallization using
full-factorial experimental design
69
of Ito et al. (Ito et al., 1983). Cells were incubated at 303 K
and 250 r.p.m.
during 96 h in a 2 L Erlenmeyer flask containing 1 L of YPHSM
modified
medium [1% (w/v) Glucose, 3% (v/v) Glycerol, 1% (w/v) Yeast
Extract and
8% (w/v) Peptone]; these conditions increased the protein
expression. Cells
were collected by centrifugation (5000 x g for 10 min at 274 K),
resuspended
in 0.1 M KH2PO4, 1.2 M sorbitol and incubated at 30ºC during 3 h
with
lyticase (2 mg per g wet weight) in order to obtain the
protoplasts (Jigami et al.
1986), from which the protein extracts were prepared as
described previously
(Becerra et al. 1998). Kl-β-Gal was purified with ANTI-FLAG M2
affinity gel
(Sigma Chemical, USA) and concentrated with ULTRA-4 (Millipore,
UFC
803024) as already reported (Rodríguez et al. 2008). The
purified protein, with
the FLAG peptide (SDYKDDDDK) attached to its N- terminus,
was
concentrated to 7 mg mL-1 in 0.05 M Tris-HCl, 0.150 M NaCl and
0.002 M
DTT. The homogeneity of the purified protein sample was analyzed
(Fig.1) by
SDS-PAGE (Laemmli 1970).
Figure 1: SDS-PAGE analysis of the purified sample of
β-galactosidase (119 kDa).
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Chapter 2
70
Crystallization
Crystallization conditions were initially explored using high
throughput
techniques with a NanoDrop robot (Innovadine Nanodrop I) and
commercially
available screens. Crystal Screen, Crystal Screen II, Crystal
Screen Lite, Salt Rx and
Index Screen from Hampton Research, PACT Suite and JCSG+ Suite
from Qiagen
and Screen Classic from Jena Biosciences were assayed using the
sitting drop
vapour diffusion method at 291 K. Drops consisting of 0.25 µL of
precipitant
and 0.25 μL of pure Kl-β-Gal (3.5 mg mL-1 in 0.05 M Tris-HCl,
0.150 M NaCl
and 0.002 M DTT) were equilibrated against 80 μL of reservoir
solution on
sitting drop microplates (Innovaplate SD-2). Small crystals grew
with the PACT
Suite screen under several conditions when 7% of glycerol (v/v)
was added to
the protein stock solution prior to the experiment set up.
Initial hits were then
tested on Cryschem (Hampton Research) sitting drop plates by
mixing 1 μL of
protein with 1 μL of precipitant solution and equilibrating
against 500 μL of
reservoir solution. Crystallization trials in the presence of
agarose were
performed adding 0.2 % agarose to the drop using a preheated
stock of 0.4 %
agarose in the precipitant solution. Crystallization conditions
were optimized
further and small plate-shaped crystals grew in 23-27% (w/v)
Polyethylene
Glycol (PEG) 3350, 0.1 M BisTris pH