─ 41 ─ 広島県内で飼育された牛が保有する牛白血病ウイルスの tax 遺伝子に関する検討 清水 和 横山 由起 桑山 勝 (受付:平成 28 年 1 月 15 日) Analysis of the Bovine leukemia virus tax protein from dairy or breeding cattles in Hiroshima Prefecture MADOKA SHIMIZU YUKI YOKOYAMA and MASARU KUWAYAMA Western Center for Livestock Hygiene Service, Hiroshima Prefecture, 1-15, Saijogojo-cho, Higashi-Hiroshima, Hiroshima, 739-0013 SUMMARY The BLV tax gene was detected in masses and white blood cells collected from bovine leukemia virus(BLV)antibody-positive Holstein and Japanese Black between April 2011 and July 2015, and differences in amino acid at position 233 (leucine or proline) , reported to be involved in lowering of the onset age, and the genotype were investigated. The amino acid in the tax gene was leucine (L233) in all samples of masses and white blood cells from Holstein. In Japanese Black, L233 and proline (P233)were detected in both masses and white blood cells in 50% of animals with a mass, respectively, and in white blood cells in 83.3 and 16.7% of animals without a mass, respectively. The geometric mean of the age of animals with a mass was 47.9 months old in the L233 type and 40.5 months old in the P233 type, showing no significant difference. The genotype was type 1 in all samples in Holstein. In Japanese Black, 75.0 and 25.0% were types 1 and 3, respectively. On comparison by the tax gene, all L233 samples were type 1 and all P233 samples were type 3. The BLV tax gene type was consistent with the genotype, and type 1, which is detected at a high rate in Holstein nationwide, was also detected at a high rate in Japanese Black, suggesting dissemination of L233-type BLV in Japanese Black in Hiroshima Prefecture. To investigate lowering of the onset age, it may be necessary to investigate the cause of the onset through various approaches including a search for the BLV tax gene utilizing materials used to diagnose the disease. ── Key words: Bovine leukemia virus, tax gene, L233 type, P233 type 広島県西部家畜保健衛生所(〒 739-0013 広島県東広島市西条御条町 1-15) 報 告
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広島県内で飼育された牛が保有する牛白血病ウイルスのtax遺伝子に関する検討
清水 和 横山 由起 桑山 勝(受付:平成 28年 1月 15日)
Analysis of the Bovine leukemia virus tax protein from dairy or breeding cattles
in Hiroshima Prefecture
MADOKA SHIMIZU YUKI YOKOYAMA and MASARU KUWAYAMA
Western Center for Livestock Hygiene Service, Hiroshima Prefecture, 1-15,
The BLV tax gene was detected in masses and white blood cells collected from bovine
leukemia virus(BLV) antibody-positive Holstein and Japanese Black between April
2011 and July 2015, and differences in amino acid at position 233 (leucine or proline), reported to be involved in lowering of the onset age, and the genotype were investigated.
The amino acid in the tax gene was leucine (L233) in all samples of masses and white
blood cells from Holstein. In Japanese Black, L233 and proline (P233) were detected
in both masses and white blood cells in 50% of animals with a mass, respectively, and
in white blood cells in 83.3 and 16.7% of animals without a mass, respectively. The
geometric mean of the age of animals with a mass was 47.9 months old in the L233 type
and 40.5 months old in the P233 type, showing no significant difference. The genotype
was type 1 in all samples in Holstein. In Japanese Black, 75.0 and 25.0% were types 1
and 3, respectively. On comparison by the tax gene, all L233 samples were type 1 and
all P233 samples were type 3. The BLV tax gene type was consistent with the genotype,
and type 1, which is detected at a high rate in Holstein nationwide, was also detected at
a high rate in Japanese Black, suggesting dissemination of L233-type BLV in Japanese
Black in Hiroshima Prefecture. To investigate lowering of the onset age, it may be
necessary to investigate the cause of the onset through various approaches including a
search for the BLV tax gene utilizing materials used to diagnose the disease.
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8) Rogers, B.B., et al.:Analysis of DNA in fresh and fixed tissue by the polymerase chain reaction, Am J Pathol, 136, 3(1990)
9) Asfaw, Y., et al.:Distribution and superinfection of bovine leukemia virus genotypes in Japan, Arch Virol, 150, 493-505(2005)