© ChromaCon AG // // the CaptureSMB ® process principle 1 Contichrom ® animation - the CaptureSMB ® process principle -

© ChromaCon AG // www.chromacon.ch // the CaptureSMB® process principle 1

Contichrom® animation- the CaptureSMB® process principle -

© ChromaCon AG // www.chromacon.ch // the CaptureSMB® process principle 2

Contichrom® & CaptureSMB® explained

© ChromaCon AG // www.chromacon.ch // the CaptureSMB® process principle 3

Contichrom® & CaptureSMB® explained

© ChromaCon AG // www.chromacon.ch // the CaptureSMB® process principle 4

Contichrom® & CaptureSMB® explained

Feed

column length

Internalconcentration

t1

t2t3

Feed

Wasted resin capacity in batch chromatography

© ChromaCon AG // www.chromacon.ch // the CaptureSMB® process principle 5

Feed

Contichrom® & CaptureSMB® explained

Step 1:•Feed & wash

Step 2:•elute product•feed column

Step 3:•CIP & re-equil.•feed column

© ChromaCon AG // www.chromacon.ch // the CaptureSMB® process principle 6

Feed

Contichrom® & CaptureSMB® explained

Step 1:•Feed & wash

Step 2:•elute product•feed column

Step 3:•CIP & re-equil.•feed column

© ChromaCon AG // www.chromacon.ch // the CaptureSMB® process principle 7

Wash

Contichrom® & CaptureSMB® explained

Step 1:•Feed & wash

Step 2:•elute product•feed column

Step 3:•CIP & re-equil.•feed column

© ChromaCon AG // www.chromacon.ch // the CaptureSMB® process principle 8

Elution

Contichrom® & CaptureSMB® explained

Feed

Product

Step 1:•Feed & wash

Step 2:•elute product•feed column

Step 3:•CIP & re-equil.•feed column

© ChromaCon AG // www.chromacon.ch // the CaptureSMB® process principle 9

Contichrom® & CaptureSMB® explained

FeedCIP &

Re-equilibrationStep 1:•Feed & wash

Step 2:•elute product•feed column

Step 3:•CIP & re-equil.•feed column

© ChromaCon AG // www.chromacon.ch // the CaptureSMB® process principle 10

Contichrom® & CaptureSMB® explained

Feed

Step 1:•Feed & wash

Step 2:•elute product•feed column

Step 3:•CIP & re-equil.•feed column

© ChromaCon AG // www.chromacon.ch // the CaptureSMB® process principle 11

Contichrom® & CaptureSMB® explained

Wash

Step 1:•Feed & wash

Step 2:•elute product•feed column

Step 3:•CIP & re-equil.•feed column

© ChromaCon AG // www.chromacon.ch // the CaptureSMB® process principle 12

Contichrom® & CaptureSMB® explained

Elution

Product

Feed

Step 1:•Feed & wash

Step 2:•elute product•feed column

Step 3:•CIP & re-equil.•feed column

© ChromaCon AG // www.chromacon.ch // the CaptureSMB® process principle 13

Contichrom® & CaptureSMB® explained

CIP & Re-equil.

Feed

Start over

Step 1:•Feed & wash

Step 2:•elute product•feed column

Step 3:•CIP & re-equil.•feed column