บบบบบ บบบบบบบ บบบบบบบบบบบบบบ บบบบบบบบบบบบบบบบบบบบบบ บบบบบบบบบบบบบบบบบบบบบบบบบ บบบบบบบบ 1 9 Julu 2014 Sujittra Phongvivat BQCLP DLD @ Maruay Hotel
Feb 23, 2016
บทบาท หนาท หองปฏบตการ ในการสนบสนนการสงออกสนคาเกษตรและ
อาหารของกรมปศสตว
19 Julu 2014Sujittra Phongvivat BQCLP DLD @ Maruay Hotel
toBureau of Quality Control of Livestock Products (BQCLP),
Department of Livestock Development
Welcome
29 Julu 2014Sujittra Phongvivat BQCLP DLD @ Maruay Hotel
3
สำ�นกตรวจสอบคณภ�พสนค�ปศสตว กรมปศสตว
ในก�รสนบสนนด�นคว�มปลอดภยอ�ห�ร บทบ�ท หน�ท
หองปฏบตก�รอ�งองตรวจสอบอ�ห�รของอ�เซยนด�นส�รตกค�งย�สตว ASEAN Food Reference Laboratory for Veterinary Drug Residue (AFRL for VDR)บทบ�ทหน�ท
สำ�นกตรวจสอบคณภ�พสนค�ปศสตว
Sujittra Phongvivat BQCLP DLD @ Maruay Hotel 9 Julu 2014
Bureau of Quality Control of Livestock Products (BQCLP)
4
91 Moo 4 Tiwanon Rd., Bangkadi sub-district, Muang district, Pathumthani province, 12000 Thailand
Tel. +66-2-967-9700 Fax. +66-2-967-9747E-mail address: [email protected]
http://dld.go.th/qcontrol
BQCLP Building 1
BQCLP Building 2
Sujittra Phongvivat BQCLP DLD @ Maruay Hotel 9 Julu 2014
BQCLP: Personnel & Budget
5
Total = 356 positions
49 official (16 veterinary officials ,1 para-veterinary official and 25 scientists, 6 administrators )4 permanent employee, 303 contracted employee (อตร�สวน ข�ร�ชก�ร : พนกง�น�ชก�ร 1 : 6)
Director; 1Expert; 3
Administrator; 6
Veterinary official; 13 Para-veteri-
nary of -ficial; 1
Scientist; 25
Perma-nent em-
ployee; 4
Contracted employee; 303
Sujittra Phongvivat BQCLP DLD @ Maruay Hotel 9 Julu 2014
0.00
50,000,000.00
100,000,000.00
150,000,000.00
200,000,000.00
250,000,000.00
300,000,000.00
42,
014,
540
85,
769,
110
25,
463,
900
153
,247
,550
50,
526,
724
77,
015,
440
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54,8
00
128
,796
,964
57,
669,
700
94,
084,
280
121
,646
,400
273
,400
,380
62,
170,
620
92,
617,
600
31,
250,
100
186
,038
,320
2011201220132014
Budg
et (b
aht)
2. บทบ�ท หน�ท
6Sujittra Phongvivat BQCLP DLD @ Maruay Hotel 9 Julu 2014
Vision วสยทศน
7
“To be an internationally recognized testing laboratory for safety and quality control of
livestock products”เปนหองปฏบตก�รอ�งองของประเทศ ทไดรบก�ร
ยอมรบในระดบส�กลในด�นก�รตรวจสอบคณภ�พสนค�ปศสตว
Sujittra Phongvivat BQCLP DLD @ Maruay Hotel 9 Julu 2014
Mission พนธกจ
8
Surveillance and monitoring livestock products for food safety control ตรวจตดต�มเฝ�ระวงด�นคว�มปลอดภยอ�ห�รของสนค�ปศสตว Providing technical assistance and training on quality and safety of
livestock products ใหคว�มชวยเหลอด�นวช�ก�ร โดยจดก�รฝกอบรมด�นคณภ�พและคว�มปลอดภยของ
สนค�ปศสตว Performing research & development for food safety and quality testing
methods ทำ�ง�นวจยและพฒน�วธวเคร�ะหด�นคณภ�พและคว�มปลอดภย Strengthening laboratory network capacity with local service laboratory
and collaboration with other outbound laboratories to meet International Standard
สร�งเครอข�ยเสรมศกยภ�พหองปฏบตก�รทใหบรก�รในพนท ใหคว�มรวมมอกบหองปฏบตก�รทงในและนอกประเทศ เพอพฒน�ศกยภ�พหองปฏบตก�รใหเปนทยอมรบต�มม�ตรฐ�นส�กล
Sujittra Phongvivat BQCLP DLD @ Maruay Hotel 9 Julu 2014
Mission พนธกจ
9
As the National Reference Laboratories for quality control testing of animal feed, veterinary medicinal products, hazardous substances and livestock products หองปฏบตก�รอ�งองระดบช�ตในด�น ก�รตรวจสอบควบคมคณภ�พอ�ห�รสตว ผลตภณฑย�สตว วตถอนตร�ย และสนค�ปศสตว
As the ASEAN Food Reference Laboratory for Veterinary Drug Residue หองปฏบตก�รอ�งองก�รวเคร�ะหอ�ห�รของอ�เซยนด�นส�รตกค�งย�สตว
Sujittra Phongvivat BQCLP DLD @ Maruay Hotel 9 Julu 2014
บทบ�ท หน�ท
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ใหบรก�รก�รตรวจสอบคณภ�พสนค�ปศสตว ด�นคว�มปลอดภยอ�ห�ร กจกรรมต�ง ๆ เชนตรวจ เฝ�ระวงส�รตกค�ง กจกรรมตรวจ
รบรองเพอสงออก เนออน�มย ฯลฯ วธวเคร�ะห (พฒน�วธ, พสจนคว�มใชได, ขอก�รรบรอง ISO/IEC
17025:2005) ฝกอบรม ถ�ยทอดเทคโนโลยก�รตรวจสอบ เพอสร�งเสรม
ศกยภ�พคว�มส�ม�รถหองปฏบตก�ร ก�รดำ�เนนก�รถ�ยโอนภ�รกจ ใหบรก�ร คำ�ปรกษ�แนะนำ� หองปฏบตก�ร ผประกอบก�ร
เกษตรกร
Sujittra Phongvivat BQCLP DLD @ Maruay Hotel 9 Julu 2014
BQCLP Organization Chart
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1. General Administration Section ฝ�ยบรห�รง�นทวไป
1. Feed Quality Control Laboratory Div. กลมตรวจสอบคณภ�พอ�ห�รสตว
2. Veterinary Drug Assay Divisionกลมตรวจสอบคณภ�พย�สตว
3. Veterinary Public Health Laboratory Div. กลมตรวจสอบคณภ�พเนอสตวและผลผลตจ�กสตว
Bureau of Quality Control
of Livestock Products3. Laboratory Quality System Division กลมพฒน�ระบบคณภ�พ
หองปฏบตก�ร
4. Milk and Milk Products Quality ControlLaboratory Div. กลมตรวจสอบคณภ�พนมและผลตภณฑนม
2. Laboratory Management Division สวนบรห�รง�นจดก�รหองปฏบตก�ร
4. Laboratory Audit Divisionกลมรบรองหองปฏบตก�ร 4 Laboratory
divisions
4 Supporting units
BQCLP
Samples for testing
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Feed & feed additive
Livestock products
Veterinary medicinal products
Fat
Sujittra Phongvivat BQCLP DLD @ Maruay Hotel 9 Julu 2014
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BQCLP Testing Activities
Farm, Manufacture of animal feed, port of entry
Farm,slaughterhouse, processing plants, port of entryDairy farm, Milk collecting center
Farmer,DLD Inspector
Authorized personnel/
veterinarian
Feed additives and
Animal feed
Meat & animal products
Milk & Milk products
Samples reception
Sample analysis
Test results
Veterinary drugs,
Hazardous Substances
Farm, Drug stores
DLD Regional officer
(Veterinarian)
DLD Traceability
database
LIMS
Test Reports
Farmer, DLD Inspector
Source of samples Sample collectors(Customer)Test samples BQCLP
Sujittra Phongvivat BQCLP DLD @ Maruay Hotel 9 Julu 2014
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1. Feed Quality Control Laboratory Division (FQCL)กลมตรวจสอบคณภ�พอ�ห�รสตว
Quality and safety
Monitoring and quality control of feed to fulfill feed quality control acts BE 2525 and additional BE 2542 (1982) and international standard (EU , FDA etc.)
Method development and method validation
Research for feed quality control and feed safety in farm
Setting the standardFeed analysis
• Quality Assurance System• Monitoring Plan
• Inspection and collection for quality control
• Research on public health implications of animal feed
RESPONSIBILITY
Sujittra Phongvivat BQCLP DLD @ Maruay Hotel 9 Julu 2014
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1. Feed Quality Control Laboratory Division (FQCL) กลมตรวจสอบคณภ�พอ�ห�รสตว
HPLC-UV
Physical inspectionMicroscopeELISACHARM II
LC-MSLC-MS/MS
Screening test
Determination
Confirmation
Analytical method & Instrummentation
Sujittra Phongvivat BQCLP DLD @ Maruay Hotel 9 Julu 2014
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Test Samples : Raw Materials, Feed, Feed Additives, Premixes, etc.
Physical : Drugs, Vitamins and Minerals Screening test, Ingredient and Adulteration
Chemical : Proximate and Nutrient analysis, Vitamins, Minerals, Urea, Urease activity and Heavy metal
Microbiological : Pathogenic bacteria, Total bacteria and Mould count, Microbiological assay, DNA&GMOs (Real-time PCR) and Probiotics
Toxicology and Biochemistry Mycotoxins : Aflatoxin, Ochratoxin, Zearalenone, Fumonisin and T-2 Toxin, Deoxynivalenol(DON)Hormone, Veterinary Drug andPesticides, PCBs, MelamineDioxin, etc.
1. Feed Quality Control Laboratory Division (FQCL)กลมตรวจสอบคณภ�พอ�ห�รสตว
Sujittra Phongvivat BQCLP DLD @ Maruay Hotel 9 Julu 2014
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2. Veterinary Drug Assay Division (VDAD) กลมตรวจสอบคณภ�พย�สตว (และวตถอนตร�ย)
RESPONSIBILITY1. Testing samples • Post-marketing veterinary
medicinal products• Hazardous substance
products• Livestock drinking water• Antimicrobial resistant
microorganisms
2. Method development & Method validation
3. Research in related fields
Sujittra Phongvivat BQCLP DLD @ Maruay Hotel 9 Julu 2014
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ANALYTICAL METHODS 1. Veterinary medicinal products• Identification• Assay of active ingredients
colistin/gentamicin/ oxytetracycline/ streptomycin/ tylosin amoxicillin/ enrofloxacin/ doxycycline/ lincomycin/ penicillin/ sulphonamide/ trimethoprim/ tiamulin albendazole/ ivermectin/ chlortetracycline/ clorsulon
• pH• Water content/Loss on
drying[Pharmacopoeia]
2. Hazardous substance products• Assay of Active
ingredients Aldehyde group Quaternary
Ammonium compounds
Hypochlorite as available chlorine
Hydrogen peroxide and Peracetic acid
• Efficiency testing Phenol coefficient/
AOAC used dilution technique
3.Prohibited substances• Determination of
prohibited substances in feed water
Nitrofurans Chloramphenicol Nitroimidazole Beta – agonist
2. Veterinary Drug Assay Division (VDAD) กลมตรวจสอบคณภ�พย�สตว (และวตถอนตร�ย)
4. Antimicrobial resistance (AMR)• Disk diffusion• MIC• PCR
Sujittra Phongvivat BQCLP DLD @ Maruay Hotel 9 Julu 2014
Annual surveillance of veterinary medicinal products quality
AFVC = Division of Animal Feed and Veterinary Products Control กองควบคมอาหารและยาสตวBQCLP = Bureau of Quality Control of Livestock Products สำานกตรวจสอบคณภาพสนคาปศสตวDLD, MOAC = Department of Livestock Development, Ministry of Agriculture and CooperativeThai FDA, MOPH = Food and Drug Administration, Ministry of Public Health
Veterinary Drug
Supplier / Farm
77 Provincial Livestock
Offices
BQCLP AFVCThai
FDA / MOPH
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2. Veterinary Drug Assay Division (VDAD) กลมตรวจสอบคณภ�พย�สตว (และวตถอนตร�ย)
DLD, MOAC
Sujittra Phongvivat BQCLP DLD @ Maruay Hotel 9 Julu 2014
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3. Veterinary Public Health Laboratory Division (VPHL) กลมตรวจสอบคณภ�พเนอสตวและผลผลตจ�กสตว
RESPONSIBILITY ภ�รกจ
1. Livestock products food safety, quality surveillance and monitoring testing for
•National residue control plan (NRCP)•Microorganisms (e.g. food hygienic indicators, food borne pathogens•Veterinary drug residues, pesticide residues, contaminants, mycotoxins•Food authenticity (e.g. species identification for Halal food)•Others:- dyes
2. As Reference Laboratory (National / ASEAN Food Reference Laboratory)Information center for test method, RM/CRM, expertsTraining centerProficiency Testing Provider (PTP) / Inter-Laboratory comparison (ILC)
3. As a leading laboratory:- Research and development for high efficient and performance test methods
Sujittra Phongvivat BQCLP DLD @ Maruay Hotel 9 Julu 2014
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3. Hygiene and Food Microbiology
Food hygienic microorganism
Food borne pathogens Microbiological Assay (6-plate test) Porcine DNA by RT-
PCRby conventional culture
method, ELISA and molecular method
2. Veterinary Drug and Hormones Residues
[ASEAN Food Reference Laboratory for Veterinary drug residues]
Screening test by ELISA, Biosensor, HPLC, etc.
Screening and confirmation by LC-MS/MS,
Multi residue screening and confirmation by
LC-QToF
1. Food Chemistry, chemical residues and contaminants
Pesticide residues analyzed by GC-ECD, HPLC-UV, GC-MS/MS, LC-MS/MS
Heavy metal analyzed by AAS, ICP-MS
Aflatoxins by HPLC-FD
Dye e.g. Canthaxantin
VPHL comprises 3 sections of laboratories
3. Veterinary Public Health Laboratory Division (VPHL) กลมตรวจสอบคณภ�พเนอสตวและผลผลตจ�กสตว
Sujittra Phongvivat BQCLP DLD @ Maruay Hotel 9 Julu 2014
4. Milk and Milk Product Quality Control Laboratory (MMPQCL) กลมตรวจสอบคณภ�พนมและผลตภณฑ
Physical
Biological
Chemical
Screening
• Reduction test• pH• Freezing point
• Standard plate count• Lab. Pasteurization count• Coliform
• Milk composition (fat, protein, etc)• Somatic cell
• Antibiotic screening test• ก�รเตมนมผงในนำ�นมดบ -Deoxyketone
22
บทบ�ทหน�ท: ตรวจควบคมมคณภ�พนำ�นมดบ ซงเปนวตถดบ ในก�รผลตนมและผลตภณฑเพอคว�มปลอดภยในก�รบรโภค เพอก�รปรบปรง ตดต�ม เฝ�ระวง คณภ�พนำ�นมระดบฟ�รม ศนยรวบรวมนำ�นมดบ ก�รขนสง เพอใหผลตภณฑนมมคณภ�พด ปลอดภย ส�ม�รถแขงขนได
Sujittra Phongvivat BQCLP DLD @ Maruay Hotel 9 Julu 2014
3. ก�รทดสอบคณภ�พนำ�นมดบ
4. ประเมนคณภ�พ ใหคำ�แนะนำ� เพอปรบปรงใหดขน
2. ศนยรวบรวมนำ�นม
Improve farm management for good quality and
better price 1. Farm
ฟ�รม
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4. Milk and Milk Product Quality Control Laboratory (MMPQCL) กลมตรวจสอบคณภ�พนมและผลตภณฑ
Sujittra Phongvivat BQCLP DLD @ Maruay Hotel 9 Julu 2014
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แผนและผลก�รตรวจตวอย�งตอป
LABORATORY
Plan/Results (Samples)2011 2012 2013 2014
VPHL 44,575 / 77,681
39,755 / 56,671
53,799 / 80,358
47,183 /
FQCL 6,786 / 7,557
6,902 / 7,111
8,569 / 8,949
8,600 /
VDAD 1,365 / 1,362
1,654 / 1,496
1,558 / 1,199
1,558 /
MQCL 24,000 / 57,576
52,000 / 55,193
52,000 / 62,997
52,000 /
Sujittra Phongvivat BQCLP DLD @ Maruay Hotel 9 Julu 2014
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Laboratory
Method development 2011
FQCL 1. Sudan Red I analysis in animal feed by LC-MS/MS
2. Inhibitory in animal feed microbiological assay sensitivity test of 5 reference bacteria with 10 antibiotics
3. Coccidiostat group : Nicarbazin analysis in animal feed by LC-MS/MS
Laboratory
Method validation 2011
FQCL 1. Deoxynivalenol analysis in chicken feed by LC-MS
2. Aflatoxins analysis in corn by Immunoaffinity column / Fluorometry3. Nitroimidazole analysis in animal feed by LC-MS/MS
4. Organochlorinated pesticides and polychlorinated l biphenyls analysis in animal feed by GC-MS/MS
5. Crude fiber analysis in chicken feed
ก�รพฒน�และก�รพสจนคว�มใชไดของวธวเคร�ะห
Sujittra Phongvivat BQCLP DLD @ Maruay Hotel 9 Julu 2014
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Laboratory
Method validation 2011 (cont)
FQCL 6. Aflatoxins analysis in chicken feed with IAC – post column photochemical reaction by HPLC – Fluorescence detection AFB1, AFB2, AFG1, AFG2
7. Cadmium analysis in corn by ICP-OES
8. Adulterant in corn meal by microscopic analysis
9. Adulterant in soybean meal by microscopic analysis
10. Tetracyclines analysis in animal feed by HPLC: oxytetracycline, chlortetracycline, doxycycline
11. Total mold count by ISO21527 – 2 : 2008 for animal feed ; Total mold count from < 10 to 10n cfu/g
Sujittra Phongvivat BQCLP DLD @ Maruay Hotel 9 Julu 2014
ก�รพฒน�และก�รพสจนคว�มใชไดของวธวเคร�ะห
27
Laboratory
Method development 2012
FQCL • Development for the detection enumeration of Enterobacteriaceae in animal feed
• Development and validation of detection protein content in corn, soybean and fish meal by NIRS
• Validation of aflatoxins analysis in cattle feed with IAC-post column photochemical reaction by HPLC-fluorescence detection
• Validation of aflatoxins analysis in chicken feed by immunoaffinity column / fluorometry
• Method validation for determination of cadmium in poultry feed by ICP-OES
• Method validation for determination of carbadox in swine feed by LC-MS/MS
Laboratory research and development
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Laboratory Method development 2013FQCL • Application of Near Infrared Spectroscopy for determination of
nutrients content in fish meal adulterated with feather meal• Development of the dioxins method in animal feed using high
resolution GC-MS• Method validation for determination of nicarbazin, salinomycin and
narasin in layer chickens feed by LC-MS/MS• Validation of aflatoxins analysis in swine feed with IAC-post column
photochemical reaction by HPLC-fluorescence detection
• Validation of zearalenone analysis in corn by immuno-affinity column / fluorometry
• Validation of aflatoxins analysis in cattle feed by immuno-affinity column / fluorometry
• Method validation for determination of cadmium in soy meal (wet digestion) by ICP-OES
• Validation of enumeration of Enterobacteriaceae in animal feed (ISO 21528-2: 2004)
• Method validation for determination of enrofloxacin in chicken feed by LC-MS/MS
Laboratory research and development
Sujittra Phongvivat BQCLP DLD @ Maruay Hotel 9 Julu 2014
Laboratory
Method development & validation 2011
VDAD2011
• Method development and validation of penicillin G and streptomycin assay in veterinary medicinal products
• Method development and validation of coumatetralyl assay in hazardous substance products for livestock
• Method development and validation for trace analysis of fluoroquinolones in livestock drinking water using LC-MS ion trap
• Method development and validation for trace analysis of Beta-agonists in livestock drinking water using LC-MS ion trap
• Method development and laboratory set-up procedure for testing of hazardous substance products
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ก�รพฒน�และก�รพสจนคว�มใชไดของวธวเคร�ะห
Sujittra Phongvivat BQCLP DLD @ Maruay Hotel 9 Julu 2014
VDAD2012
• Method validation for amitraz determination in hazardous substance products using
HPLC-DAD• Method development for active ingredient assay of hazardous substance
products• Method development and laboratory set-up procedure for testing of hazardous
substance productsVDAD2013
• Method development for phenolic compounds determination in hazardous substances products for livestock using HPLC-DAD
• Method validation for amitraz determination in hazardous substance products for livestock using HPLC-DAD
• Method development and capacity building of hazardous substance products for livestock testing laboratory
Laboratory
Method development & validation 2011
VPHL • Determination of mercury and lead contaminant in animal tissue and liver
2011 • Determination of Aminoglycosides in honey by LC-MS/MS
• Study on application of real-time ultrasound for screening Beta-agonist abused in finishing pigs
30
ก�รพฒน�และก�รพสจนคว�มใชไดของวธวเคร�ะห
Sujittra Phongvivat BQCLP DLD @ Maruay Hotel 9 Julu 2014
VPHL2012
• Development method for the determination of 16 PAHs in sausage by GC-MS/MS
• Method development of spinosad in animal fat analysis by HPLC-UVD
• Method development and validation for confirmation of aminoglycosides in pig muscle by LC-MS/MS
VPHL2013
• Method development for confirmation of tetracyclines residues in honey by LC-MS/MS
• Development and method validation for determination of meat contaminations using Real-time PCR
• Validation for confirmatory method for tetracyclines residues in honey by LC-MS/MS
• Method development for confirmation of amitraz residues in honey by GC-MS/MS
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2. In-house Calibration Laboratory ใหบรก�รก�รสอบเทยบ เครองแกว เครองชง ทใชใน สตส (ISO/IEC 17025:2005 accredited)
1. Laboratory Quality System Section พฒน�ระบบคณภ�พหองปฏบตก�รของ สตส ใหเปนไปต�มขอกำ�หนดทวไปว�ดวยคว�มส�ม�รถของหองปฏบตก�รทดสอบและหองปฏบตก�รสอบเทยบ
(ISO/IEC 17025:2005)
Laboratory quality system development division กลมพฒน�ระบบหองปฏบตก�ร
Sujittra Phongvivat BQCLP DLD @ Maruay Hotel 9 Julu 2014
Laboratory section Testing scope
Food Chemistry, Residues and Contaminants
7
Veterinary Drug and Hormone Residues 6Food Hygiene and Microbiology 15Veterinary Drugs and Hazardous Substances Assay
5
Feed Quality Control 15Calibration 2
Total 50
Laboratory Accreditation (ISO/IEC 17025:2005)
32Sujittra Phongvivat BQCLP DLD @ Maruay Hotel 9 Julu 2014
Laboratory Section
Testing Scope (ISO/IEC 17025
Accredited)Type of Sample
Food Chemistry, Residues and Contaminants
- Total Arsenic- Cadmium- Lead- Total Mercury
Liver, MuscleHoney
- Organochlorine Pesticides (OCPs)
Animal fat
ISO/IEC 17025:2005 Accreditation
33Sujittra Phongvivat BQCLP DLD @ Maruay Hotel 9 Julu 2014
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Laboratory Section
Testing Scope(ISO/IEC 17025
Accredited)Type of Sample
Veterinary Drug and Hormone Residues
- Sulfonamides Muscle
- Protein-bound Nitrofurans Metabolites
- Animal tissue and processed animal tissue
- Egg- Honey
- Beta-agonist Brombuterol, Cimaterol,
Cimbuterol, Clenbuterol, Hydroxymethylclenbuterol, Mabuterol, Mapenterol, Ractopamine, Salbutamol and Zilpaterol
- Urine- Muscle- Liver
ISO/IEC 17025:2005 Accreditation
Sujittra Phongvivat BQCLP DLD @ Maruay Hotel 9 Julu 2014
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Laboratory Section
Testing Scope(ISO/IEC 17025
Accredited)Type of Sample
Veterinary Drug and Hormone Residues
- Chloramphenicol - Animal tissue
- Egg- Honey- Milk
- Fluoroquinolones; Enrofoxacin and
Ciprofloxacin
- Muscle- Egg
- Tetracyclines; Oxytetracycline, Tetracycline, Chlortetracycline Doxycycline
- Muscle
ISO/IEC 17025:2005 Accreditation
Sujittra Phongvivat BQCLP DLD @ Maruay Hotel 9 Julu 2014
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Laboratory Section
Testing Scope(ISO/IEC 17025
Accredited)Type of Sample
Food Hygiene and Microbiology
- Anti-bacterial substances residues
- Clostridium perfringens - Escherichia coli and
Coliform- Enterococcus - Enterococcus (in water and
ice) - Enterobacteriaceae- Listeria monocytogenase- Salmonella spp.- Staphylococcus aureus (BPK)- Staphylococcus aureus (RPF)- Staphylococal enterotoxin- Total bacterial count- Yeast & Mould- Presumptive Determination
of Escherichia coli (Water and Ice)
- Determination of Salmonella spp.
Meat and Meat Products
Laboratory Accreditation
Sujittra Phongvivat BQCLP DLD @ Maruay Hotel 9 Julu 2014
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Laboratory Section
Testing Scope
(ISO/IEC 17025 Accredited)
Type of Sample
Feed quality control - Aflatoxins in corn- Total Aflatoxins in corn- Adulteration feather meal
in fish meal- Adulteration in soybean
by microscope- Adulteration in corn by
microscope- Determination of
Cadmium in soybean meal
- Determination of Cadmium in corn by ICP-OES
Raw material and animal feed
Laboratory Accreditation
Sujittra Phongvivat BQCLP DLD @ Maruay Hotel 9 Julu 2014
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Laboratory Section
Testing Scope
(ISO/IEC 17025 Accredited)
Type of Sample
Feed quality control
- Antibiotic inhibitory microbiological assay
- Beta-agonist in complete feed for swine
- Fiber in poultry feed- Protein in poultry feed- Salmonella spp. in feed
and animal feeding stuffs- Tetracycline,
Oxytetracycline Chlotetracycline, Doxycycline in poultry feed
- Total plate count in feed and animal feeding stuffs
Raw material and animal feed
ISO/IEC 17025:2005 Accreditation
Sujittra Phongvivat BQCLP DLD @ Maruay Hotel 9 Julu 2014
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Laboratory Section
Testing Scope
(ISO/IEC 17025 Accredited)
Type of Sample
Veterinary Drugs and Hazardous Substances Assay
- Quality assay of enrofloxacin drug using HPLC
- Determination of Beta-agonist drug in livestock water using LC-MS iontrap
- Tylosin
- pH
- Use dilution
Veterinary medicinal productsLivestock drinking
water
Injectable Solution
Veterinary medicinal
Disinfectant
ISO/IEC 17025:2005 Accreditation
Sujittra Phongvivat BQCLP DLD @ Maruay Hotel 9 Julu 2014
หองปฏบตก�รเอกชนทผ�นเกณฑก�รประเมนและไดรบมอบก�ร ถ�ยโอนภ�รกจแลว จำ�นวน 47 หองปฏบตก�ร
ป พ.ศ. 2554 จำ�นวน 26 หองปฏบตก�ร ป พ.ศ. 2555 จำ�นวน 12 หองปฏบตก�ร ป พ.ศ. 2556 จำ�นวน 9 หองปฏบตก�รTotal 47 laboratories
กลมรบรองหองปฏบตก�รก�รดำ�เนนก�รถ�ยโอนภ�รกจ ต�มมตครม 11 พ.ค. 2553
40Sujittra Phongvivat BQCLP DLD @ Maruay Hotel 9 Julu 2014
43
• AFRLs network was initiated at the Workshop and Expert Consultation on “Strategies for Strengthening Food Testing in ASEAN”
In Jakarta, Indonesia on 7 – 11 June 2004 under the EC- ASEAN Economic cooperation Programme on Standards, Quality and Conformity Assessment.
• Veterinary Public Health Lab was selected and assigned as AFRL for Veterinary Drug Residues since then.
ASEAN Food Reference Laboratory for Veterinary Drug Residues Since 2004
International Activities
Sujittra Phongvivat BQCLP DLD @ Maruay Hotel 9 Julu 2014
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AFRL for VDR activities
Provide training & technical advice & services to ASEAN Member Countries (AMCs)
Become resource & information center i.e. test method for vet drug residues, experts, Certified Reference Materials/ Reference Materials (CRM/RM)
Coordinate inter-laboratory comparisons or proficiency testing scheme for veterinary drug residues
Strengthening laboratory cooperation network for consumer protection
International Activities
Sujittra Phongvivat BQCLP DLD @ Maruay Hotel 9 Julu 2014
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BQCLP :- VDAD – AHRI (Taiwan) Building Capacity of Veterinary Drugs Testing Laboratory between Thailand and Taiwan (R.O.C) Since 2011 (study visit)
18 - 22 September 2012 3 person
22 - 26 July 20132 person
9 - 15 May 2011 2 person
International Activities
Sujittra Phongvivat BQCLP DLD @ Maruay Hotel 9 Julu 2014
BQCLP staff participation in international committee
CODEX Committee of Food Contaminants
CODEX Committee of Food Hygiene (CCFH)
CODEX Committee on Residue of Veterinary Drugs in Food (CCRVDF)
CODEX Committee on Method of Analysis and Sampling (CCMAS)
CCFH Physical Working Group on Revision of the Principles for the Establishment and Application of Microbiological Criteria for Foods
ISO Technical Committee for Microbiology : ISO/TC 34/SC9 Meeting
34/SC9 MeetingISO Technical Committee for Horizontal Methods for Molecular Biomarker Analysis : ISO/TC 34/SC16 MeetingSujittra Phongvivat BQCLP DLD @
Maruay Hotel 469 Julu 2014
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BQCLP as a learning organization (LO) through knowledge management (KM):-
BQCLP encourages our staff to continually self studying and to improve their working performances:-By - Internet accessible - Library - Internal broadcasting - In house training - Attending seminar, conference &
workshop by close collaboration/ communication through
- Coffee time open discussion - Knowledge/best practice sharing
through - Community of Practitioner (CoP), KM
forum by production of work manual/SOP to be followed for highest quality of work.
BQCLP as a Learning Organization
Sujittra Phongvivat BQCLP DLD @ Maruay Hotel 9 Julu 2014
4. Laboratory plan: 2014-2018
48Sujittra Phongvivat BQCLP DLD @ Maruay Hotel 9 Julu 2014
1. Extending scope of accredited method
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Plan / Year 2014 2015 2016 2017 2018FQCL 1. Testing method in animal feed 3 scopes 3 scopes VDAD 1. Testing method in VD & HS & feed water 3 scopes 2 scopes VPHL 1. Pesticide residues in animal fat 2 scopes
2. Nitrofuran metabolites residue (Total residues) in egg and honey using LC-MS/MS
1 scope
3. Sulfonamides residue in honey using LC-MS/MS 1 scope
4. Avermectin using HPLC-FD 1 scope
5. Campylobacter in food 1 scope 6. Testing method for veterinary drug residues in meat
and meat product 1 scope 1 scope
MMPQC 1. Aerobic plate count in milk (raw and processed) 1 scope 2. Coliform in milk (raw and processed) 1 scope 3. Testing method in milk (raw and processed) 3 scopes
Sujittra Phongvivat BQCLP DLD @ Maruay Hotel 9 Julu 2014
2. Method development and validation
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Plan / Year 2014 2015 2016 2017 2018FQCL 1. Dioxin in meat and bone meal / (MD) 2. GMOs in meat and bone meal / (MD) 3. Species identification in meat and bone meal / (MD) 4. Color additive in animal feed / (MD) 5. Testing method for heavy metals and veterinary drugs
in animal feed / (MD)
6. Molecular method development for DNA base sequencing using MALDI-TOF MS
/ (MD)
7. Testing method development for heavy metals and mineral using ICP-MS
/ (MD)
VDAD 1. Veterinary drugs & Hazardous substance products 2 (MD/MV) 2 (MD/MV) 2 (MD/MV) 2 (MD/MV) 2 (MD/MV)
2. Hazardous substances laboratory development 4 items 4 items 4 items 4 items 4 itemsVPHL 1. Arsenic speciation analysis in food of animal origin by
ICP-MS1 (MD) 1 (MV)
2. Veterinary drug residue by LC-MS/MS and LC-QToF
1 (MD/MV) 1 (MD/MV)
9 Julu 2014Sujittra Phongvivat BQCLP DLD @ Maruay Hotel
3. Laboratory facilities development
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Plan / Year 2014 2015 2016 2017 2018FQCL 1. Bio-molecular laboratory for animal feed testing / 2. Rapid alert and monitoring system development for animal feed
safety /
3. Sample preparation for inter-laboratory comparison of contamination of veterinary drugs, mycotoxins and heavy metals in animal feed
/
4. Reference animal feed quality testing laboratory (i.e. adulteration, mycotoxins, nutrition enrichment (protein, fat, fiber, moisture, etc.))
/
5. Training center for analysis of contaminant in animal feed and corrective action for feed quality control of Thailand
/
VDAD 1. Monitoring and surveillance project for antimicrobial resistant
microorganism in livestock products/ (pilot) / / / /
VPHL 1. PT scheme for veterinary drug residues (round/year) 1 1 2 2 2 2. Microbiological assay interlaboratory comparison (round/year) 1 1 1 1 1
MMPQC 1. Ring test for milk and milk products quality control laboratory
(round/year)4 4 4 4 4
9 Julu 2014Sujittra Phongvivat BQCLP DLD @ Maruay Hotel
4. Training course
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Plan / Year 2014 2015 2016 2017 2018VDAD 1. Pharmaceutical analysis workshop 1 1 1 1 1 2. Antimicrobial resistant (AMR) workshop 1 1 VPHL 1. Hygiene and food microbiological training course 1 1 1 1 1 2. Veterinary drug residue analysis/ AFRL veterinary drug
residue workshop1 1 1
MMPQC 1. Milk and milk products quality testing course 1 1 1 1 1
Sujittra Phongvivat BQCLP DLD @ Maruay Hotel 9 Julu 2014
5. Capacity building project & Testing service
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Plan / Year 2014 2015 2016 2017 2018VDAD 1. Capacity Development of Veterinary Drugs Testing
Laboratory between Thailand and Taiwan (R.O.C) /
2. Capacity Building for Testing of Hazardous Substances in Livestock Production Testing Laboratory under TCPF with FAO
/ /
3. Plan to propose for twinning laboratory project (Veterinary medicinal products) with OIE Collaborating Center (France)
/
4. Project Proposal Application for Enhancing National Capacities for Antimicrobial Resistance Risk Management in Food Animal Production in Thailand with FAO (ongoing)
/
Sujittra Phongvivat BQCLP DLD @ Maruay Hotel 9 Julu 2014
Plan / Year 2014 2015 2016 2017 2018VDAD
Efficiency of disinfectant by Use-dilution /
T h a n k y o u
54Sujittra Phongvivat BQCLP DLD @ Maruay Hotel 9 Julu 2014