醫學檢驗及生物技術概論 疾病感控及實驗室生物安全

96.10.26

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(199923)(Center for Disease Control)

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II

III

(National Health Commend Center)-----

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-1015,0007,500-(1)(2)(3)(4)(5)(6)(7)(8)

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Sheet1

8887

66

3228

5461

(,,,)14

181186

Sheet2

Sheet3

BSL-3

92-94 95-99 94-97

96/09/13

Sheet1

96

39223.0

(C)(D=F/C)(E=(G+H)/C)(F)(G)

0361,100,00099.04%16.11%60,515,6359,841,431

05-148,192,00098.62%33.85%47,524,8145,860,183

05-284,006,000102.33%20.37%85,961,96017,111,703

0613,468,00091.91%27.86%12,378,7543,751,759

0123,249,50426.85%14.36%6,241,4783,337,499

230,015,50492.44%17.35%212,622,64139,902,575

(C)(D=F/C)(E=G/C)(F)(G)

0324,800,00089.92%0.79%22,300,296196,560

05-133,360,00089.64%2.64%29,902,725880,700

05-217,640,00038.38%0.95%6,770,800167,200

061,900,00080.56%41.06%1,530,650780,150

011,511,30064.26%7.46%971,200112,800

79,211,30077.61%2.70%61,475,6712,137,410

Sheet2

20062007

154,27196,591

15,11513,672

5234

12()

Sheet3

Two virology Laboratories

1. Viral Enteric and Emerging Diseases Laboratory2. Viral Respiratory Diseases Laboratory Being responsible for research and diagnoses on influenza, SARS, measles, mumps, VZV, rubella, parovovirus B19, RSV, adenovirus, hMPV, enteroviruses, rotavirus, norovirus, astrovirus, HAV, HBV, HCV, HDV, HEV, HIV-1/HIV-2, and EID (rabies, ebola, nipah, and smallpox).Current research projects:Surveillance on diagnostic quality of HIV and hepatitis B/CMonitoring AFP syndromeSetting up a surveillance network for enterovirus infectionsDeveloping a swift molecular biological diagnostic system for viral respiratory track infections

Two Bacterial Laboratories

1. Bacterial Respiratory Diseases Laboratory 2. Bacterial Enteric and Emerging Diseases Laboratory

Diagnoses of notifiable bacterial communicable diseases, including Cholera, Plague, Anthrax, Diphtheria, Meningococcal meningitis, Typhoid fever, Paratyphoid fever, Bacillary dysentery, Enterohemorrhagic Escherichia coli infection, Pertussis, Scarlet fever, Legionellosis, Haemophilus influenzae type B infection.Diagnoses of non-notifiable bacterial communicable diseases, including Salmonellosis, Melioidosis, Yersiniosis, Lyme disease, Leptospirosis, cat scratch disease, and diseases caused by Campylobacter spp., Vibrio parahaemolyticus, Staphyolcoccus aureus, Bacillus cereus.Researches related to the above bacterial pathogens.

Vector-Borne Viral and Rickettsial Diseases LaboratoryResponsible for diagnoses and related research of yellow fever, Dengue, Japanese encephalitis, other flaviviruses (WNV, etc.), Hantavirus, scrub typhus (mite-borne), typhus fever (louse-borne), Q fever, and others (spotted fever, etc.).Ongoing research projects:Establishing a flaviviruse reference laboratory for Asian-Pacific region.Dengue fever sero-epidemiological survey in southern part of Taiwan.Setting up a fluorescence quantitative molecular diagnostic system for rickettsial infectious diseases.Setting up a serological diagnostic system for rickettsia.

Mycotic Diseases Laboratory Diagnoses and research of Pathogenic fungi Chlamydia and Mycoplasma. Parasitic Diseases Laboratory Diagnoses and research of malaria andamebic dysentery.

Mycobacterial Diseases Laboratory Diagnoses, identification and research of mycobacteria.External quality control of contract laboratory diagnoses.Vector Biology Laboratory Identification, classification, and survey of disease vector insects. Research in pesticides and their application forbest maintaining environment hygiene. Studying the influence of aerosol size on the effectiveness of killing mosquitoes by the pesticide.

WHO - National Influenza Centres(National Influenza Centre, NIC) WHO(WHO global influenza surveillance network, GISN)1948WHO- GISN118 NIC( 89)4WHO (Collaborating centres, CCs)

NICWHO

NIC

Taiwan NIC

11

Taiwan NIC

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5*34113O8522966041761,928488***0116 ***0604A11,0962966621762,23073201740906A21,0962966621762,2301,0962966621762,230B3,090531 **1,464 **2644,704

WHO 1979WHO1986A/Taiwan/1/86 WHO

2005NIIDWHO2006-07A/Taiwan/567/2005

(Biohazard)(Laboratory Biosafety) (Biosecurity)

-() (Aerosol)

RG = Risk Group

BSL = Biosafety Level

(Primary containment)()(Secondary containment)()

(BSC)Class IClass IIClass III

94 9 26 0940000614 19 95 3 26 95 4 11 0950000194 2 1 19

23,4 16,1712567811910,131415 192.1181

3 4 7 15 10 13 3 11 12 3

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BSL-3 RG3 BSL-3 BSL-3

SARS

SARS 92 12 SARS RT-PCR SARS BSL-3 SARS CDC NIID BSL-3

93 4 Real-time RT-PCR ()

Dengue I 5NCR-C-prM-M-E-NS1-NS2A-NS2B-NS3-NS4A-NS4B-NS5-3NCR (10735 bp)Structural gene Non-structural gene

NS5 (154 bp) C-prM (435 bp) Virus strain used in the lab100%100%Virus strain isolated from Mainland China in 1980 (n=1)100%99.5%Virus isolated from Kao-Ping area in 1987~1988 (n=10)100%99.3~100%Virus isolated from Kaohsung County in 1991 (n=3)100%99.1~99.3%

95 8 (Pulsed-Field Gel Electrophoresis, PFGE) DNA

vs. vs. vs.