Why Cryogenic Grinding?

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Why Cryogenic Grinding?

• Many analytical samples are too flexible or sensitive to be ground at ambient temperatures

• Samples are subject to degrading• Does not damage DNA or RNA

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The Basics

• Samples are chilled at Liquid Nitrogen (LN) temperatures and ground using a Magnetically-Driven Impactor– No mechanical linkages or bearings to fail

• Sealed Grinding Vials ensure sample integrity

• Touch screen display w/ stored procedures for rapid process replication and consistency

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Who Can Benefit From a Freezer/Mill®?

• Forensic Labs – DNA identification• Medical Labs– genetic research, disease

prevention• Hospital Labs – For genetic screening• Agricultural Labs – For crop development• Agricultural Veterinary Labs – For livestock

breeding and health• Archealogy Labs – Ancient DNA extraction• Public Health and Environmental Monitoring Labs• QC Testing of Manufactured Goods• Electronics Companies – Grinding Electronic

Components

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The Freezer/Mill® Family

Sample Size

0.6 – 5 g 0.6 – 100 g 0.6 – 200 g

LN System

Manual Manual, Auto-Fill Option Auto-Fill Only

Price $ $$ $$$

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6970EFM – Features & Benefits

• Two Models Available– Single Grinding Chamber (6970M)

• 1 Grinding Chamber + 1 Pre-Cooling Chamber

– Dual Grinding Chambers (6970D)

• Totally enclosed auto-fill system with no Liquid Nitrogen exposure

• Increased through-put– Up to 8 small vials or 2 large vials

(D model only)

© SPEX SamplePrep, Inc. 2011

6770 – Features & Benefits

• Cost efficient• Runs one Micro-vial or small vial per run• Perfect for smaller samples (5 grams or less)• Additional area for pre-cooling samples • Unit has small counter footprint

© SPEX SamplePrep, Inc. 2011

6870 – Features & Benefits

• Versatile large grinding chamber– Runs a variety of vials, ranging from micro vials

through the large, even up to 4 of the small vials– Allows for wide range of sample sizes

• Available with Auto-Fill, or without

• Additional area for pre-cooling samples

© SPEX SamplePrep, Inc. 2011

Freezer/Mill Accessories

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Freezer/MillVials & Vial Sets

Micro Small Mid-Size Large

0.1 – 0.5 mL 0.5 – 4.0 mL Up to 25 mL Up to 50 mL

0.1 – 0.5 g 0.5 – 4.0 g Up to 40 g Up to 100 g

Vial Sizes

Vial Materials• Standard Set

• Stainless Steel End Plugs & Impactor with Polycarbonate Center Cylinder• Polycarbonate

• Avoids metal contamination• Stainless Steel

• Best for Polymer applications• Cr-Free

• For RoHS/WEEE appliactions

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Freezer/MillAuto-Extractor

6814 Auto-Extractor™• Works with Small and Large

size vials• Increases throughput• Reduces operator effort

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Freezer/MillAccessory Packages

6870S

6870L

6870MIncludes:

• 6754 Small Extractor• 6755 Vial Rack• 6806 Multi-Vial

AdapterIncludes:

• 6884 Mid-Size Adapter for 6804 Large Extractor

• 6886 Mid-Size Grinding Vial Adapter

Includes:

• 6804 Large Extractor• 6805 Vial Rack

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Freezer/MillLiquid Nitrogen Handling

• Cryogenic Gloves

Liquid Nitrogen• Portable Dewars & Pressurized Tanks

– Required for use with Auto-Fill units(6870 + Auto-Fill & 6970EFM)

Safety

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Samples, Application Notes & Resources

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Before & After…

Leather

Corn

BoneA Collection of our Before &

After Samples

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Application Notes

• Extensive library of Freezer/Mill® applications

Examples• SP005 – RNA Extraction from Cartilage

Tissue Using Cryogenic Grinding• SP006 – Preparation of Rice Husks for

the Analysis of Their Contents• SP007 – Grinding Polymers For

Qualitative and Quantitative Analysis

Also Available:

Featured Articles Library

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Product Demonstration Videos