TrueClone - OriGene · success story, Novartis scientists utilized OriGene’s TrueClone Collection to identify novel gene regulators of the WNT signaling pathway 1. OriGene is
Post on 15-Feb-2019
221 Views
Preview:
Transcript
2005 – 2006 PRODUCT CATALOG
TrueClone:Authentic Full-Length cDNA Clones
Dear Valued Customer,
The completion of the sequencing of the human genome provides us with an enormous amount of data
related to individual genes and their transcripts. There remains, however, a large informational gap between
a gene’s sequence and its biological function, and an even greater gap between the gene’s function and its
complicated interactions with other genes. Unraveling and understanding gene functions and gene networks
will undoubtedly be a great challenge for some time to come.
In 1997, OriGene formulated a vision that the availability of a physical collection of all human genes will be
required to dissect gene function and genetic interactions. After over eight years of dedication, OriGene scientists
have isolated more than 24,000 distinct human full-length cDNA clones representing over 15,000 loci, all in a
unified pCMV expression ready vector. We have built the world’s largest searchable collection of cDNA clones,
the TrueClone CollectionTM. We are proud of the fact that thousands of innovative researchers worldwide have
been taking advantage of these ready-made clones to accelerate their research.
The value of the TrueClone collection is multifaceted:
1. As a source of individual genes. When an authentic human full-length cDNA clone is readily available,
scientists are alleviated of the tedious work of gene cloning and can better direct their time and resources
to more exciting discoveries downstream.
2. As a collection of gene families. For laboratories with relatively focused interests, the TrueClone collection
can be used as an inventory for scientists to pick-and-assemble their own sub-collections and conduct
functional assays. OriGene has pre-assembled several such sub-collections based on biological pathways
or gene families, including protein kinases, GPCR’s, nuclear hormone receptors, and cytochrome
P-450 genes.
3. As a comprehensive collection. Expression-ready TrueClones provide unprecedented opportunities for
scientists to functionally test thousands of human genes in a uniformed cell based assay.This approach,
which we call FlagArray (Full-Length cDNA Annotation of Gene functions), was recently employed by
scientists at the Genomics Institute of the Novartis Research Foundation. Using 20,000 cDNA clones,
including a large number of OriGene TrueClones, they successfully discovered and characterized several
novel modulators of the Wnt signaling pathway.1
OriGene is dedicated to its mission of isolating every single human full-length cDNA clone that represents a
functional transcript in the human genome. We are determined to make TrueClone the ultimate collection: where
every gene can be found, and that every scientist can trust. We understand this is a formidable task. To adhere to
the highest quality standards when isolating authentic clones is costly and time consuming; yet OriGene is
committed to this effort. This strong commitment, coupled with our diverse scientific expertise, has made the
TrueClone collection not only the most comprehensive available, but also the highest quality source. We welcome
all suggestions from our customers to 1) enhance our gene collection 2) provide more extensive downstream
applications and 3) improve our product support. We are especially interested in developing innovative
technologies that functionally assay the human genome in its entirety. If you have an idea, an invention or a
research program potentially involving large subsets of our collection of human full-length cDNA clones, please
do not hesitate to contact us. The perfect collection of human full-length cDNA clones can only be attained via input
and feedback from scientists like you. We look forward to earning your trust and to being your discovery partner.
From the OriGene Team
1. Identification of the Wnt signaling activator leucine-rich repeat in Flightless interaction protein 2 by a genome-wide functional analysis. Liu, J. et al. PNAS, Feb 2005; 102: 1927 - 1932.
ii www.origene.com
Tab
leo
fC
on
ten
ts
Sections
I. TrueClone™—Ready-cloned non-PCR human full-length cDNA in CMV vectors..................................................................1
Human full-length genes..................................................................................................2
CloneSet: Gene-family based collection .......................................................................10
• Kinase CloneSet
• GPCR CloneSet
• Nuclear Hormone Receptor CloneSet
• Ion Channel CloneSet
• P450 CloneSet
II. HuSH™ ..........................................................................................................45Premade shRNA expression panels ..............................................................................46
pRS vectors......................................................................................................................61
III. Full-length cDNA cloning.............................................................................63Rapid-Screen™ arrayed cDNA libraries ........................................................................64
• Human cDNA Libraries
• Mouse cDNA Libraries
• Rat cDNA Libraries
Rapid-Screen™ Longest-CloneSM services ....................................................................72
• Human 12-Tissue Library Screen
• Mouse 6-Tissue Library Screen
Sure-RACE™ 5’-RACE Kit ...............................................................................................75
• Human 12-Tissue cDNA
• Mouse 12-Tissue cDNA
Yeast two-hybrid expression cDNA libraries ................................................................81
• DupLEX-A™ yeast two-hybrid system kit
• Human Libraries
• Mouse Libraries
• Rat Libraries
• Breast Cancer Libraries
• Prostate Cancer Libraries
• Other Libraries
iiiph: 1.888.267.4436 fax: 1.301.340.9254
Tab
leo
fC
on
ten
ts
IV. Gene expression ...........................................................................................85Rapid-Scan™: PCR-based gene expression profiling panel........................................86
• Human Tissue Panel (24 Tissues)
• Human Brain Panel (12 Parts)
• Human Breast Cancer (24 Normals/Tumors)
• Mouse Tissue Panel (24 Tissues)
• Mouse Brain Panel (48 Parts/Stages)
• Drosophila Tissue Panel (12 Parts/Stages)
Real-Time-Scan™: Real-Time PCR-based gene expression profiling panel ..............86
• Human Tissue Panel: (48 tissues) NEW
Northern Blot...................................................................................................................95
• Human 12-Tissue RNA Blot I
• Human 12-Tissue RNA Blot II
• Human 6-tissue RNA Blot III
• Mouse 12-Tissue RNA Blot
• Rat 12-Tissue RNA Blot
• Rat 12 Brain-Part RNA Blot
Multiple-Choice™ cDNAs .............................................................................................101
• Human Tissue cDNA
• Mouse Tissue cDNA
• Rat Tissue cDNA
Blue-Ribbon™ Poly A+ and Total RNAs........................................................................105
• Human Tissue RNA
• Mouse Tissue RNA
• Rat Tissue RNA
V. Miscellaneous molecular tools..................................................................109Pre-validated antibodies ...............................................................................................110
• Kinase antibodies
• GPCR antibodies
Protein collections ........................................................................................................130
Quanti-Ladder .............................................................................................................. 140
Broad range DNA size marker (200bp-10kb) with quantity reference
Millennium™ marker ....................................................................................................141
Broad range RNA size marker (0.5kb-9kb)
Rapid-Load.....................................................................................................................142
Innovative PCR compatible loading buffer
Ordering information .......................................................................................143
iv www.origene.com
TrueClone™Ready-cloned non-PCR human full-length cDNA in CMV vectors
Over 24,000 individual clones
CloneSet™: Gene-family based collection
Tru
eClo
ne™
Read
y-Cl
oned
Non
-PCR
hum
anFu
ll-Le
ngth
cDN
Ain
CMV
vect
ors
Ready-cloned non-PCR human full-length
cDNA in CMV vectors
2 www.origene.com
All TrueClones are fully searchable using the search
engine on our website. Each clone is represented by
a unique catalog number of 6 digits with a prefix of
“TC”.
OriGene’s TrueClone Collection contains over 24,000
full-length cDNA clones whose nucleotide sequences
can be found in the public domain. They represent
about 85 percent of the transcript sequences that
have been annotated by NCBI.
OriGene’s mission is to clone every single human
full-length transcript. Our cloning effort is aimed at
expanding TrueClone coverage until our mission is
accomplished. Please visit our website often for the
most updated status. If your gene of interest is not
yet included in our collection, you are encouraged to
fill out a cloning request form and our scientists will
make every effort to clone your gene for you.
ADVANTAGES
The most comprehension collection: over 24,000 (and growing)
cDNA clones available
Authentic clones: no PCR artifacts
Expression-Ready: All clones areexpression-optimized in a CMV vector
Quick delivery: Most clones will be shipped within 48 hrs
Product Listing
OriGene’s 24,000 human full-length cDNA clones have individual catalog numbers beginning with TC100001
and growing. Each clone is priced to reflect the respective cloning effort needed to produce it. All of
OriGene’s clones are priced to provide value to our customers in the scientific community.
Visit our website at www.origene.com for price and specifications for each clone.
Tru
eClo
ne™
Read
y-Cl
oned
Non
-PCR
hum
anFu
ll-Le
ngth
cDN
Ain
CMV
vect
ors
Introduction:
Functional genomics, the development and
application of experimental approaches to assess
gene function, begins with a full-length copy of the
gene of interest. Historically, gene cloning was done
one gene at a time through painstaking efforts from
individual laboratories. Organizations such as NCBI,
DDBJ, and EMBL have been centralizing and
archiving gene sequence information, yet they
cannot provide the physical clones needed to allow
researchers to pursue the functional dissection of
these genes, many of which still code for
hypothetical proteins having no known functions.
OriGene Technologies is focused on its mission to
clone every single human gene transcript in the
human transcriptome. We believe that a complete set
of human cDNAs provides the key to unlocking the
fundamentals of human development, physiology
and disease. Since 1997, OriGene Technologies has
been diligent in isolating full-length human genes
from primary cDNA libraries. Utilizing high-
throughput cloning and proprietary Rapid-Screen™
technologies, OriGene has built its TrueCloneTM
Collection — the world’s largest searchable set of
over 24,000 non-redundant full-length human cDNA
clones suitable for protein expression in mammalian
cells or in vitro through coupled translation assays.
All TrueClones are matched to annotated mRNA
reference sequences found in the public domain.
Beginning in 2001, OriGene was one of the first com-
panies to provide ready-cloned genes to the research
community. Our dependable service and dedicated
support have relieved many scientists of the tedious
work of traditional gene cloning. Today, purchasing a
clone has become the standard way of obtaining a
gene; it greatly shortens the gene-cloning timetable
and allows scientists to concentrate their efforts and
resources on exciting downstream applications.
Most importantly, the availability of a large number
of expression-ready human full-length cDNAs makes
it possible to perform global screening of the entire
human transcriptome. Such a systematic approach
has been pioneered by several groups and has
shown to be extremely effective1-8. In one recent
success story, Novartis scientists utilized OriGene’s
TrueClone Collection to identify novel gene
regulators of the WNT signaling pathway1. OriGene is
releasing several such gene array tools to provide
this enabling technology to researchers. The
FlagArrayTM (Full-Length cDNA Annotation of Gene
function array) is one such tool.
3ph: 1.888.267.4436 fax: 1.301.340.9254
1. Identification of the Wnt signaling activator leucine-rich repeat in Flightless interactionprotein 2 by a genome-wide functional analysis Proc Natl Acad Sci U S A. 2005 Feb 8;102(6):1927-32. (Use of over 10,000 OriGeneTrueClones)
2. High throughput functional genomics: identification of novel genes with tumorsuppressor phenotypes. Int J Cancer. 2005 Jan 20;113(3):434-9. (Use of over 17,000 clones)
3. High-throughput Functional Genomics Identifies Genes That Ameliorate Toxicity Due toOxidative Stress in Neuronal HT-22 Cells: GFPT2 Protects Cells Against PeroxideMol Cell Proteomics. 2004 Aug;3(8):834-40. (Use of 5,000 clones, including OriGene'sTrueClones)
4. Identification of p53 regulators by genome-wide functional analysisProc Natl Acad Sci U S A. 2004 Mar 9;101(10):3456-61. (Use of 20,000 clones)
5. Genome-scale functional profiling of the mammalian AP-1 signaling pathway.Proc Natl Acad Sci U S A. 2003 Oct 14;100(21):12153-8. (Use of 20,000 clones)
6. Identification of a family of cAMP response element-binding protein coactivators bygenome-scale functional analysis in mammalian cells.Proc Natl Acad Sci U S A. 2003 Oct 14;100(21):12147-52. (Use of 20,000 clones)
7. An integrated functional genomics screening program reveals a role for BMP-9 inglucose homeostasis.Nat Biotechnol. 2003 Mar;21(3):294-301. (Use of 8,000 clones encoding secretedproteins)
8. TIP, a T-cell factor identified using high-throughput screening increases survival in agraft-versus-host disease model. Nat Biotechnol. 2003 Mar;21(3):302-7. (Use of 8,000 ESTs encoding putative secretedproteins)
Product Description:
What is a TrueClone?
TrueClone is the world’s largest collection of full-
length human cDNA clones, and is available only
from OriGene. The TrueClone Collection has the
following key features:
1. Comprehensive collection: The TrueClone col-
lection currently contains over 24,000 non-
redundant human cDNAs, each corresponding to
a unique transcript. It is by far the largest source
of human cDNA clones. OriGene has the
commitment and the technical strength to clone
every single human gene.
2. Authentic clones: More than 99% of the clones are
directly isolated from primary cDNA libraries with
no PCR-introduced artifacts. A very small number of
clones are generated by PCR and these are all fully
sequenced to match to their references. These PCR
clones are noted as such on the OriGene website.
3. Expression ready: All genes are directionally
cloned into an expression vector under control of
the CMV promoter, ready to be transfected into
mammalian cells for protein expression. The
uniform vector in all clones facilitates high-
throughput screening approaches.
4. Easy search: OriGene provides several search
engines on our website for convenient identification
of your gene of interest. Please refer to page 7 to see
more search information.
5. Quick delivery: Most TrueClones will be delivered
to you as dried, plasmid DNA within 48 hrs in 2-D
bar-coded tubes.
How is a full-length cDNA defined in OriGene’s TrueClone collection?
Full-length is usually defined as containing the full
open reading frame (ORF) of a gene, but OriGene
TrueClones contain 5’- and 3’-UTRs as well. Currently
OriGene’s clones are defined as full-length when
they satisfy the following three specifications.
1. 5’-junction sequencing: The sequence aligns
upstream of the initiation ATG codon.
2. 3’-junction sequencing: The sequence aligns
downstream of the stop codon.
3. Size verification: Each insert is removed from the
vector by restriction digestion and the size is esti-
mated via gel electrophoresis. This size is matched
against the predicted size of the full-length clone.
OriGene makes all 5’ sequence data available
through its website to facilitate customer searches. In
addition, OriGene has sequenced the ORF of more
than 2,000 TrueClones, including most of the protein
kinases, and has made this information available on
our website. The number of ORF sequenced clones
will increase steadily in the coming years.
How is a non-PCR clone different from aPCR-based clone? Can PCR be used inTrueClone’s downstream applications?
PCR clones may contain mutations that cause
downstream experimental deviations. Non-PCR clones
reflect the true nature of mRNA gene transcripts.
It is a well-documented fact that the PCR process
introduces mutations due to the limited fidelity of Taq
or other PCR enzymes. When a gene is obtained by
the PCR process from a cDNA pool, especially when
the transcript is rare, the high replication cycle
causes the accumulation of mutations. However, this
mutation rate can pale in comparison to the
introduction of mutations due to primer synthesis
errors that can affect 14% of all such PCR clones9.
Even when the final transcripts are sequenced, there
is no way to differentiate whether a mutation is due
to PCR or is simply a naturally occurring SNP. In
addition, very long genes and GC-rich genes can be
impossible to PCR amplify.
One key feature of TrueClones is that they are
authentic clones, isolated directly from cDNA
libraries constructed by a high-fidelity reverse
transcriptase. When using TrueClones, there is no
need to worry about PCR-introduced artifacts.
PCR can be utilized in TrueClone applications.
Starting with an authentic cDNA clone and knowing
Tru
eClo
ne™
Read
y-Cl
oned
Non
-PCR
hum
anFu
ll-Le
ngth
cDN
Ain
CMV
vect
ors
4 www.origene.com
9. Human ORFeome version 1.1: a platform for reverse proteomics. Genome Res. 2004 Oct;14(10B):2128-35.
Tru
eClo
ne™
Read
y-Cl
oned
Non
-PCR
hum
anFu
ll-Le
ngth
cDN
Ain
CMV
vect
ors
its sequence, molecular biologists can safely apply
PCR to amplify a segment of the clone, either the ORF
or a functional domain, for other applications. Using
larger starting amounts of the template DNA, PCR
can be performed with a very low cycle number,
reducing the chance of mutation. More importantly,
by having the sequence information of the template
DNA, it is easy to verify the sequence of the final
product and then to distinguish any PCR-introduced
mutations from naturally occurring SNPs.
How many genes are there in the humangenome? Which commercial source offersthe most complete set of humantranscript clones?
The most recent human genome build (#35) boasts a
large scale improvement over all previous builds and
is likely to become the working genome for quite
some time, providing a more stable gene reference
set. While the total number of gene loci is currently
believed to be 21,787 (2,188 predicted), the expected
number of transcripts including splice variants is
expected to be 1.54 times that, or 33,552.
The largest commercialized collection of cDNA
clones is OriGene’s TrueClone Collection: ~24,000
transcripts covering over 15,000 loci. This is more
than twice as many as those represented in the
mammalian gene collection. Our comprehensive
collection not only provides individual gene users
the best source to find their gene, but also provides
the finest system biology tool available to assess
drug target cross-reactivity, drug action and antigen
recognition. Providing the complete human
transcriptome as non-PCR clones is and remains
OriGene’s mission.
Fig. 1 illustrates the relative relationship between the
predicted human genome and current coverage
provided by OriGene’s TrueClone collection and MGC.
OriGene’sTrueClone collection not only contains more
human full-length cDNA clones than any other
collection, but it also contains more of the difficult and
long clones. Since most cDNA collections all overlap
Fig. 1
Comparison of the predicted number of human transcripts and the
coverage of OriGene TrueClone and MGC. OriGene has more full
length cDNA clones representing a greater number of transcripts
than any other source.
*Number of Transcripts including: Genes, Predicted Genes, Pseudo-
genes and Splice Variants
26,200 (RefSeq)
24,000 OriGene
>33,000 transcripts (21,787 loci)*Human Genome Build 35
12,242 (MGC)
5ph: 1.888.267.4436 fax: 1.301.340.9254
Tru
eClo
ne™
Read
y-Cl
oned
Non
-PCR
hum
anFu
ll-Le
ngth
cDN
Ain
CMV
vect
ors
6 www.origene.com
in their coverage of highly abundant and small cDNA
clones (ORF size less than 3kb), we looked at 200
difficult to isolate clones in this druggable genome
that have an ORF greater than 4 kb.
The result of this analysis is as follows: OriGene has
about 120 of these 200 cDNAs. One of the larger
commercial reagent suppliers has about 27 of these
and the MGC collection has 17 cDNAs representing
these 200 genes. (Fig. 2)
What vectors are employed by TrueClones,and how do they compare to otherexpression vectors?
All of the cDNAs within the TrueClone collection are
cloned into a series of pCMV6 vectors, allowing for
uniform expression screening methods. The vector
plasmid was originally obtained from Dr. David
Russell’s laboratory10,11. The full-length cDNA frag-
ments are directionally inserted downstream from a
eukaryotic transcriptional promoter capable of
driving heterologous gene expression in a variety of
mammalian cell lines and transgenic mice.
Vector Diagram
All three OriGene pCMV6 vectors were constructed
with the same features and multiple cloning site
(MCS), with the exception that pCMV-XL6 has an SP6
transcriptional promoter instead of T7. The basic
backbone of the vector series is presented in the
diagram to the left. (Fig. 3)
Extensive work has been done to engineer the vector
to achieve a high level of transgene expression.
When compared with the popular expression
plasmid, pCDNA3.1 (Invitrogen), pCMV-based
plasmids provide comparable if not higher levels of
transgene expression. (Fig. 4)
Key Functional Features of pCMV6 vectors:
• Vector size: 4.7kb
• Selection marker in E. coli: Ampicillin-resistance
• Selection marker in mammalian cells: None;
requires co-transfection for selection; perfect for
transient transfections
• Promoter for in vivo expression in mammalian
cells: CMV promoter
10. Cloning, structure, and expression of the mitochondrial cytochrome P-450 sterol 26-hydroxylase, a bile acid biosynthetic enzyme. J Biol Chem. 1989 May 15; 264(14):8222-9. 11. Expression cloning and regulation of steroid 5 alpha-reductase, an enzyme essentialfor male sexual differentiation. J Biol Chem. 1989 Sep 25; 264(27):16249-55.
Comparison of transgene expression levels in pCMV6- and
pCDNA3.1-based plasmids. CAT gene was cloned downstream of
the promoters in the pCMV6 and pCDNA3.1 vectors. In three
independent experiments, the same quantity of plasmid DNA was
transfected into Hela cells and the CAT activity was scored.
T7 Promoter
Sac INot IEcoR I#Bgl IIKpn IEcoRVHind IIISal I#*Xba INot ISma I
# Cloning sites*Lost upon cloning
f1 ori
Amp resistancegene
ColE1 SV40 ori
PolyA signal
cmv promoter
pCMV6-XL44707 bp
Fig. 3
Fig. 4
MCS
Fig. 2
Druggable Genome extracted from information provided by Hopkins,
AL, and Groom, CR, The Druggable Genome, Nat Rev Drug Discov.
2002 9:727-30. In our evaluation, the Druggable Genome consists of
over 3800 unique genes, of which 200 are larger than 4 kb in length.
The comparison above is based upon those large genes.
Fig. 5
Sample applications for individual TrueClone.
Tru
eClo
ne™
Read
y-Cl
oned
Non
-PCR
hum
anFu
ll-Le
ngth
cDN
Ain
CMV
vect
ors
7ph: 1.888.267.4436 fax: 1.301.340.9254
• Promoter for in vitro cell free system:T7 (for pCMV6-
XL4 and pCMV6-XL5) and SP6 for (pCMV6-XL6)
• Cloning sites: EcoRI and SalI; while EcoRI is
preserved, SalI is destroyed upon cloning
• Restriction sites for removing insert: NotI. Two NotI
sites are flanking the vector’s cloning sites
• Cell lines suitable for transfection: COS, HEK293,
Hela, CHO, NIH3T3, Mouse L cell, etc.
• Transcription termination and poly-adenylation
signals: From human growth hormone (hGH) gene
The complete sequence of pCMV6-XL4 has been de-
posited in Genbank with accession number AF067196.
All vector sequences are available at www.origene.com.
What are the utilities of the TrueClone?
TrueClones can be utilized as:
• Individual clones
• Bundled clones
• A complete collection
Individual TrueClones enable a wide spectrum of
gene function studies, as shown in Fig. 5.
Bundles of TrueClone can be selected to perform
focused screening for ligand binding, protein
interaction, or pathway dissection. The bundle can be
constructed based on gene families, such as protein
kinases, GPCRs, nuclear hormone receptors, etc, or it
can be based on functional or cellular localization
similarities, such as secreted molecules, membrane
proteins, etc. OriGene has grouped some key gene
families into CloneSets™ to facilitate such needs.
The complete TrueClone collection can be used to
perform global screening. The comprehensive nature
of the TrueClone and its uniform expression vector
platform make it an ideal tool for a system biology
approach. Exciting new discoveries12, 13 have been
recently made using the TrueClone collection.
How do I search for a gene?
OriGene’s website www.origene.com provides a
wide choice of search criteria.
12. Identification of the Wnt signaling activator leucine-rich repeat in Flightlessinteraction protein 2 by a genome-wide functional analysis. Proc Natl Acad Sci U S A. 2005 Feb 8;102(6):1927-32. 13. High-throughput Functional Genomics Identifies Genes That Ameliorate Toxicity Dueto Oxidative Stress in Neuronal HT-22 Cells: GFPT2 Protects Cells Against Peroxide Mol Cell Proteomics. 2004 Aug;3 (8):834-40.
Producing siRNA by in vitro transcriptionand dicer digestion
Subclone into viral vector for
virus production (eg. Adenovirus, Lentivirus, etc)
proteinexpression in cell-free
system (eg. TNT)
Transient in vivo protein
expression
Subcloning intoother expression
vectors for taggedexpression
TrueClone
Knock-inconstruct fortransgenicexperiment
Transfection ofmammalian cells
Antibody validation
Tru
eClo
ne™
Read
y-Cl
oned
Non
-PCR
hum
anFu
ll-Le
ngth
cDN
Ain
CMV
vect
ors
8 www.origene.com
• BLAST by Nucleotide Sequence, or BLAST by
Protein Sequence
The most sensitive search method is via
BLAST. It generates the most definitive match
to your gene of interest.
• Accession or Catalog Number
The most specific method is by NCBI
accession number. OriGene generally uses the
RefSeq reference number beginning with NM
or XM whenever it is available.
• Protein Domain
An effective method to extract lists of genes
that share a common protein domain.
• Keywords
Keywords are the most flexible but can also
fail to give the desired result. False negative
results are common and it is recommended
that a BLAST search be performed after a
negative keyword search.
• Gene Family
You can view OriGene’s pre-bundled Clone-
Sets™ by gene family from this selection.
If you have difficulty searching or finding what you
are looking for, please call our Customer Care Team
at 888.267.4436 or write to Search Help at OriGene
(searchhelp@origene.com).
Will OriGene provide a custom cloning service whenever a search result is negative?
OriGene is dedicated to clone every human
transcript and we can incorporate your needs in this
effort. When your search result is negative, you are
encouraged to fill out a “Customer Cloning Request
Form” so that our cloning team can put your clone
into our cloning queue. Given the large number of
such requests, we prioritize those customers who
make a commitment to purchase once the clone is
available. You can make such a commitment by
submitting a purchase order. To obtain a quotation,
please contact Customer Care at 1-888-267-4436.
Why are TrueClones priced differently?
Not all clones are created equally. Gene transcripts
differ greatly in many aspects, such as message
abundance, transcript length, GC content, and tissue
distribution. The respective cloning efforts needed to
produce them differ accordingly. OriGene’s
differential pricing reflects the relative difficulty in
obtaining these clones, while still providing value to
all clone users.
Some of the clones represent short and very abundant
transcripts, such as the beta-actin transcript. Such
clones can be obtained with relative ease by high-
throughput sequencing of a small number of clones in
the libraries. Most of the clones in MGC represent such
transcripts. The majority of the TrueClone collection
categorized within this group are priced at $195.
Large and rare clones are difficult to clone and
require individualized effort. Equipped with the
proprietary Rapid-ScreenTM technology, OriGene has
been able to obtain more elusive clones than any
other cloning team, and thus can provide a much
more comprehensive collection. We are continuing
to apply new technologies to clone more genes and
to expand our collection. We believe our offering
provides an excellent value to our customers.
What is provided when I order a gene?
1. OriGene provides purified plasmid DNA (200ng)
dried onto the bottom of a siliconized poly-
propylene tube. The tube has a 2-D bar code on the
bottom which ensures inventory integrity and
accurate shipping, and allows for potential
downstream integration into the end-users LIMS.
2. A copy of our TrueClone Application Guide with
information regarding DNA recovery and down-
stream applications.
3. A certificate of analysis stating your clone’s
specifications.
4. A vial of vector primer (vp1.5) for 5’ sequence
confirmation.
Will OriGene guarantee that a TrueClone is identical to a published reference?
No. There are three factors that contribute to
differences:
Tru
eClo
ne™
Read
y-Cl
oned
Non
-PCR
hum
anFu
ll-Le
ngth
cDN
Ain
CMV
vect
ors
9ph: 1.888.267.4436 fax: 1.301.340.9254
1. Single Nucleotide Polymorphisms (SNP’s). No two
collections will contain exactly the same gene
sequences, and this is true when comparing the
NCBI database references with OriGene’s True-
Clones. Given that polymorphisms exist in 0.1% of
the euchromatic genome, we can expect there to
be a 1 bp difference in each 1000 bp of a reference
on average. Obtained directly from human primary
cDNA libraries, TrueClones are the closest copies to
actual human mRNA transcripts that technology
currently allows, and therefore any such difference
in a TrueClone is likely to be a single nucleotide
polymorphism (SNP).
2. Reference sequences are not always correct. Some
references contain PCR-cloning errors and/or
sequencing errors. In fact, less than one-half of the
curated NCBI RefSeq database is currently placed
into the “reviewed, validated” category.
3. Alternative splicing. Most of OriGene’s clones are
not yet fully sequenced. We qualify our clones by
5’- and 3‘-end sequence matching and insert size
verification. It is possible that some TrueClones
contain a different splice pattern than the
reference.
OriGene guarantees that TrueClones are authentic
cDNA transcripts that encode full-length proteins. If
any of our clones are shown to contain a mutation/
insertion/deletion that disrupts the protein’s open
reading frame, every effort will be made to supply an
appropriate replacement clone from our very large
archive of full-length candidates.
For protein purification, do OriGeneTrueClones provide a fused tag to the expressed protein?
Currently TrueClones are “raw” clones from libraries
supplied without any fused tag. Customers are
encouraged to perform the vector construction in
their own labs. To satisfy such needs for tagged
protein expression, OriGene has teamed up with
Promega to develop a new line of TrueClone
derivatives—the FlexClone series. Flex clones will
enable convenient shuffling of an ORF into a variety
of vectors, facilitating multiple applications,
including tagged protein expression.
Which cloning sites were used to create TrueClones? Which restriction sites can be used to remove the insertfrom a TrueClone?
The cDNA prepared for each library construction was
engineered to have an EcoR1 site on the 5’ end and
an Xho1 site on the 3’ end. The fragments were
ligated into the pCMV vectors pre-cut with EcoR1 and
Sal1. The compatible ends of Xho1 and Sal1 created
a new site that cannot be cut with either.
To liberate the insert, use NotI. NotI sites were
engineered on both sides of the cloning sites.
Please refer to page 6 for more vector information.
What additional information is providedon OriGene’s website when I search for a clone?
OriGene is striving to be your one-stop gene cloning
resource. When you search for a gene on our site, we
provide:
• Direct links to public databases for more detailed
information about each gene
• Sequence specifications for the OriGene clone
• A 24-tissue expression profile: Relative
expression levels of this gene in 24-normal
tissues (data obtained by RT-PCR using OriGene’s
Rapid-Scan expression panels)
• All available products related to this gene,
including protein, antibody and pre-made shRNA
constructs
• The gene family, pathway and function summary
for your gene
Tru
eClo
ne™
Clon
eSet
:Gen
e-fa
mily
base
dco
llect
ion
10 www.origene.com
What is a CloneSet™? How manyCloneSets™ does OriGene have? What are the utilities of a CloneSet™?
CloneSets™ are pre-bundled clones based on gene
families. Currently there are several such Clone-
Sets™ within OriGene’s TrueClone collection:
• Protein kinase
• GPCR (G-protein coupled receptors)
• NHR (Nuclear Hormone receptors)
• P450
• Ion Channel
OriGene has created, by far, the most com-
prehensive gene family collections available in the
marketplace, and is proud to offer these important
CloneSets™ to the research community. Such
categorization assists researchers with focused
scientific interest. CloneSets™ are available as a
single product item for focused functional screening.
OriGene CloneSets™ in development include:
• Phosphatases
• Protease
• Lipid kinases
• Phosphodiesterases
• Membrane proteins
• Secreted molecules
To purchase a complete CloneSet, please contact us
at 1.888.267.4436 for terms and quotation.
CloneSet™: Gene-family
based collection
Tru
eClo
ne™
Clon
eSet
:Gen
e-fa
mily
base
dco
llect
ion
11ph: 1.888.267.4436 fax: 1.301.340.9254
Kinase CloneSetCat. No. Accession No. Description
TC107659 AB000409 Homo sapiens mRNA for MNK1, complete cds
TC107660 AB023216 Homo sapiens mRNA for KIAA0999 protein, partial cds
TC107655 AB037781 Homo sapiens mRNA for KIAA1360 protein, partial cds
TC107442 AB058693 Homo sapiens mRNA for KIAA1790 protein, partial cds
TC107661 AF308302 Homo sapiens serologically defined breast cancer antigen NY-BR-96 mRNA, complete cds
TC105333 AK074305 Homo sapiens cDNA FLJ23725 fis, clone HEP14024
TC104797 AK092352 Homo sapiens cDNA FLJ35033 fis, clone OCBBF2016590, weakly similar to CELL SURFACE ANTIGEN
114/A10 PRECURSOR
TC124162 AY792511 Homo sapiens leucine-rich repeat kinase 2 (LRRK2) mRNA, complete cds
TC124163 AY792511 Homo sapiens leucine-rich repeat kinase 2 (LRRK2) mRNA, OriGene unique variant 1
TC124164 AY792511 Homo sapiens leucine-rich repeat kinase 2 (LRRK2) mRNA, OriGene unique variant 2
TC123778 BC002695 Homo sapiens, Similar to KIAA1048 protein, clone MGC:3535 IMAGE:3607681, complete cds
TC107754 BC002695 Homo sapiens, Similar to KIAA1048 protein, clone MGC:3535 IMAGE:3607681, complete cds
TC107753 BC038807 Homo sapiens, Similar to amyotrophic lateral sclerosis 2 (juvenile) chromosome region, candidate 7,
clone MGC:46456 IMAGE:5200755, complete cds
TC107656 BC039243 Homo sapiens, Similar to fibroblast growth factor receptor 2 (bacteria-expressed kinase, keratinocyte
growth factor receptor, craniofacial dysostosis 1, Crouzon syndrome, Pfeiffer syndrome, Jackson-Weiss
syndrome), clone MGC:32962 IMAGE:5277896, com
TC107755 BC041668 Homo sapiens, Similar to receptor-interacting serine-threonine kinase 3, clone MGC:47689 IMAGE:
5767146, complete cds
TC107662 BC052239 Homo sapiens cDNA clone IMAGE:5092935, partial cds
TC116664 NM_000020 Homo sapiens activin A receptor type II-like 1 (ACVRL1)
TC115442 NM_000061 Homo sapiens Bruton agammaglobulinemia tyrosine kinase (BTK)
TC112998 NM_000075 Homo sapiens cyclin-dependent kinase 4 (CDK4)
TC113109 NM_000075 Homo sapiens cyclin-dependent kinase 4 (CDK4), OriGene unique variant 1
TC111932 NM_000141 Homo sapiens fibroblast growth factor receptor 2 (bacteria-expressed kinase, keratinocyte growth
factor receptor, craniofacial dysostosis 1, Crouzon syndrome, Pfeiffer syndrome, Jackson-Weiss
syndrome) (FGFR2), transcript variant 1
TC109231 NM_000142 Homo sapiens fibroblast growth factor receptor 3 (achondroplasia, thanatophoric dwarfism) (FGFR3),
transcript variant 1
TC109267 NM_000162 Homo sapiens glucokinase (hexokinase 4, maturity onset diabetes of the young 2) (GCK), transcript
variant 1
TC120059 NM_000215 Homo sapiens Janus kinase 3 (a protein tyrosine kinase, leukocyte) (JAK3)
TC120061 NM_000222 Homo sapiens v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog (KIT)
TC124046 NM_000245 Homo sapiens met proto-oncogene (hepatocyte growth factor receptor) (MET)
TC119991 NM_000294 Homo sapiens phosphorylase kinase, gamma 2 (testis) (PHKG2)
TC119992 NM_000294 Homo sapiens phosphorylase kinase, gamma 2 (testis) (PHKG2), OriGene unique variant 1
TC119871 NM_000455 Homo sapiens serine/threonine kinase 11 (Peutz-Jeghers syndrome) (STK11)
TC119843 NM_000459 Homo sapiens TEK tyrosine kinase, endothelial (venous malformations, multiple cutaneous
and mucosal) (TEK)
TC109227 NM_000604 Homo sapiens fibroblast growth factor receptor 1 (fms-related tyrosine kinase 2, Pfeiffer syndrome)
(FGFR1), OriGene unique variant 1
TC124152 NM_000875 Homo sapiens insulin-like growth factor 1 receptor (IGF1R)
TC119602 NM_000906 Homo sapiens natriuretic peptide receptor A/guanylate cyclase A (atrionatriuretic peptide receptor A)
(NPR1)
TC107274 NM_001079 Homo sapiens zeta-chain (TCR) associated protein kinase 70kDa (ZAP70), transcript variant 1
TC119434 NM_001105 Homo sapiens activin A receptor, type I (ACVR1)
TC119435 NM_001106 Homo sapiens activin A receptor, type IIB (ACVR2B)
TC119393 NM_001203 Homo sapiens bone morphogenetic protein receptor, type IB (BMPR1B)
Tru
eClo
ne™
Clon
eSet
:Gen
e-fa
mily
base
dco
llect
ion
12 www.origene.com
Cat. No. Accession No. Description
TC108987 NM_001204 Homo sapiens bone morphogenetic protein receptor, type II (serine/threonine kinase) (BMPR2),
transcript variant 1
TC119395 NM_001211 Homo sapiens BUB1 budding uninhibited by benzimidazoles 1 homolog beta (yeast) (BUB1B)
TC108757 NM_001221 Homo sapiens calcium/calmodulin-dependent protein kinase (CaM kinase) II delta (CAMK2D),
transcript variant 3
TC119340 NM_001258 Homo sapiens cyclin-dependent kinase 3 (CDK3), OriGene unique variant 1
TC119343 NM_001260 Homo sapiens cyclin-dependent kinase 8 (CDK8)
TC119344 NM_001261 Homo sapiens cyclin-dependent kinase 9 (CDC2-related kinase) (CDK9)
TC119354 NM_001274 Homo sapiens CHK1 checkpoint homolog (S. pombe) (CHEK1), OriGene unique variant 1
TC119355 NM_001274 Homo sapiens CHK1 checkpoint homolog (S. pombe) (CHEK1)
TC119358 NM_001278 Homo sapiens conserved helix-loop-helix ubiquitous kinase (CHUK)
TC124169 NM_001315 Homo sapiens mitogen-activated protein kinase 14 (MAPK14), transcript variant 1
TC119302 NM_001319 Homo sapiens casein kinase 1, gamma 2 (CSNK1G2)
TC119321 NM_001348 Homo sapiens death-associated protein kinase 3 (DAPK3)
TC119139 NM_001569 Homo sapiens interleukin-1 receptor-associated kinase 1 (IRAK1)
TC110934 NM_001570 Homo sapiens interleukin-1 receptor-associated kinase 2 (IRAK2)
TC119156 NM_001616 Homo sapiens activin A receptor, type II (ACVR2)
TC119158 NM_001619 Homo sapiens adrenergic, beta, receptor kinase 1 (ADRBK1)
TC119161 NM_001626 Homo sapiens v-akt murine thymoma viral oncogene homolog 2 (AKT2)
TC119127 NM_001654 Homo sapiens v-raf murine sarcoma 3611 viral oncogene homolog (ARAF)
TC119055 NM_001715 Homo sapiens B lymphoid tyrosine kinase (BLK), OriGene unique variant 1
TC119056 NM_001715 Homo sapiens B lymphoid tyrosine kinase (BLK)
TC119060 NM_001721 Homo sapiens BMX non-receptor tyrosine kinase (BMX)
TC110839 NM_001726 Homo sapiens bromodomain, testis-specific (BRDT), transcript variant 2
TC119077 NM_001744 Homo sapiens calcium/calmodulin-dependent protein kinase IV (CAMK4)
TC111605 NM_001786 Homo sapiens cell division cycle 2, G1 to S and G2 to M (CDC2), transcript variant 1
TC119016 NM_001787 Homo sapiens cell division cycle 2-like 1 (PITSLRE proteins) (CDC2L1), transcript variant 1
TC109059 NM_001798 Homo sapiens cyclin-dependent kinase 2 (CDK2), OriGene unique variant 1
TC109060 NM_001798 Homo sapiens cyclin-dependent kinase 2 (CDK2), transcript variant 1
TC119024 NM_001799 Homo sapiens cyclin-dependent kinase 7 (MO15 homolog, Xenopus laevis, cdk-activating kinase) (CDK7)
TC118967 NM_001892 Homo sapiens casein kinase 1, alpha 1 (CSNK1A1)
TC118968 NM_001893 Homo sapiens casein kinase 1, delta (CSNK1D), OriGene unique variant 1
TC109127 NM_001895 Homo sapiens casein kinase 2, alpha 1 polypeptide (CSNK2A1), transcript variant 2
TC118969 NM_001896 Homo sapiens casein kinase 2, alpha prime polypeptide (CSNK2A2)
TC123974 NM_001954 Homo sapiens discoidin domain receptor family, member 1 (DDR1), transcript variant 2
TC118918 NM_001982 Homo sapiens v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian) (ERBB3), transcript
variant 1
TC118883 NM_002005 Homo sapiens feline sarcoma oncogene (FES)
TC111632 NM_002011 Homo sapiens fibroblast growth factor receptor 4 (FGFR4), transcript variant 1
TC118894 NM_002020 Homo sapiens fms-related tyrosine kinase 4 (FLT4), transcript variant 2
TC118895 NM_002020 Homo sapiens fms-related tyrosine kinase 4 (FLT4), OriGene unique variant 1
TC118862 NM_002037 Homo sapiens FYN oncogene related to SRC, FGR, YES (FYN), transcript variant 1
TC108487 NM_002082 Homo sapiens G protein-coupled receptor kinase 6 (GRK6), transcript variant 2
TC118824 NM_002093 Homo sapiens glycogen synthase kinase 3 beta (GSK3B), OriGene unique variant 1
TC118825 NM_002093 Homo sapiens glycogen synthase kinase 3 beta (GSK3B), OriGene unique variant 2
TC118826 NM_002093 Homo sapiens glycogen synthase kinase 3 beta (GSK3B)
TC118838 NM_002110 Homo sapiens hemopoietic cell kinase (HCK)
TC118761 NM_002227 Homo sapiens Janus kinase 1 (a protein tyrosine kinase) (JAK1)
TC118716 NM_002253 Homo sapiens kinase insert domain receptor (a type III receptor tyrosine kinase) (KDR)
Tru
eClo
ne™
Clon
eSet
:Gen
e-fa
mily
base
dco
llect
ion
13ph: 1.888.267.4436 fax: 1.301.340.9254
Cat. No. Accession No. Description
TC118711 NM_002314 Homo sapiens LIM domain kinase 1 (LIMK1), transcript variant 1
TC123883 NM_002314 Homo sapiens LIM domain kinase 1 (LIMK1), transcript variant 1
TC108490 NM_002350 Homo sapiens v-yes-1 Yamaguchi sarcoma viral related oncogene homolog (LYN), OriGene unique
variant 1
TC108492 NM_002350 Homo sapiens v-yes-1 Yamaguchi sarcoma viral related oncogene homolog (LYN)
TC118702 NM_002376 Homo sapiens MAP/microtubule affinity-regulating kinase 3 (MARK3), OriGene unique variant 1
TC118703 NM_002376 Homo sapiens MAP/microtubule affinity-regulating kinase 3 (MARK3)
TC118669 NM_002401 Homo sapiens mitogen-activated protein kinase kinase kinase 3 (MAP3K3), transcript variant 2
TC110949 NM_002419 Homo sapiens mitogen-activated protein kinase kinase kinase 11 (MAP3K11)
TC118630 NM_002446 Homo sapiens mitogen-activated protein kinase kinase kinase 10 (MAP3K10)
TC108482 NM_002497 Homo sapiens NIMA (never in mitosis gene a)-related kinase 2 (NEK2)
TC109479 NM_002498 Homo sapiens NIMA (never in mitosis gene a)-related kinase 3 (NEK3), OriGene unique variant 1
TC109481 NM_002498 Homo sapiens NIMA (never in mitosis gene a)-related kinase 3 (NEK3), transcript variant 1
TC118578 NM_002530 Homo sapiens neurotrophic tyrosine kinase, receptor, type 3 (NTRK3)
TC118579 NM_002530 Homo sapiens neurotrophic tyrosine kinase, receptor, type 3 (NTRK3), OriGene unique variant 1
TC107950 NM_002576 Homo sapiens p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast) (PAK1)
TC118562 NM_002577 Homo sapiens p21 (CDKN1A)-activated kinase 2 (PAK2)
TC118563 NM_002578 Homo sapiens p21 (CDKN1A)-activated kinase 3 (PAK3)
TC118532 NM_002595 Homo sapiens PCTAIRE protein kinase 2 (PCTK2)
TC118538 NM_002610 Homo sapiens pyruvate dehydrogenase kinase, isoenzyme 1 (PDK1), nuclear gene encoding
mitochondrial protein, OriGene unique variant 1
TC118539 NM_002610 Homo sapiens pyruvate dehydrogenase kinase, isoenzyme 1 (PDK1), nuclear gene encoding
mitochondrial protein
TC118540 NM_002611 Homo sapiens pyruvate dehydrogenase kinase, isoenzyme 2 (PDK2)
TC118542 NM_002612 Homo sapiens pyruvate dehydrogenase kinase, isoenzyme 4 (PDK4)
TC118543 NM_002613 Homo sapiens 3-phosphoinositide dependent protein kinase-1 (PDPK1), OriGene unique variant 1
TC118544 NM_002613 Homo sapiens 3-phosphoinositide dependent protein kinase-1 (PDPK1)
TC110975 NM_002648 Homo sapiens pim-1 oncogene (PIM1)
TC118448 NM_002730 Homo sapiens protein kinase, cAMP-dependent, catalytic, alpha (PRKACA), transcript variant 1
TC108498 NM_002731 Homo sapiens protein kinase, cAMP-dependent, catalytic, beta (PRKACB), OriGene unique variant 1
TC108499 NM_002731 Homo sapiens protein kinase, cAMP-dependent, catalytic, beta (PRKACB), transcript variant 2
TC118449 NM_002732 Homo sapiens protein kinase, cAMP-dependent, catalytic, gamma (PRKACG)
TC124082 NM_002737 Homo sapiens protein kinase C, alpha (PRKCA)
TC118454 NM_002738 Homo sapiens protein kinase C, beta 1 (PRKCB1), transcript variant 2
TC112717 NM_002739 Homo sapiens protein kinase C, gamma (PRKCG)
TC118455 NM_002740 Homo sapiens protein kinase C, iota (PRKCI)
TC118456 NM_002741 Homo sapiens protein kinase N1 (PKN1), transcript variant 2
TC118457 NM_002742 Homo sapiens protein kinase D1 (PRKD1)
TC118459 NM_002744 Homo sapiens protein kinase C, zeta (PRKCZ), OriGene unique variant 1
TC118460 NM_002744 Homo sapiens protein kinase C, zeta (PRKCZ), OriGene unique variant 2
TC118461 NM_002744 Homo sapiens protein kinase C, zeta (PRKCZ)
TC118463 NM_002747 Homo sapiens mitogen-activated protein kinase 4 (MAPK4)
TC118465 NM_002748 Homo sapiens mitogen-activated protein kinase 6 (MAPK6)
TC109619 NM_002750 Homo sapiens mitogen-activated protein kinase 8 (MAPK8), transcript variant 2
TC118466 NM_002751 Homo sapiens mitogen-activated protein kinase 11 (MAPK11), transcript variant 1
TC111675 NM_002752 Homo sapiens mitogen-activated protein kinase 9 (MAPK9), transcript variant 1
TC109625 NM_002753 Homo sapiens mitogen-activated protein kinase 10 (MAPK10), transcript variant 1
TC118423 NM_002754 Homo sapiens mitogen-activated protein kinase 13 (MAPK13)
TC118424 NM_002755 Homo sapiens mitogen-activated protein kinase kinase 1 (MAP2K1)
Tru
eClo
ne™
Clon
eSet
:Gen
e-fa
mily
base
dco
llect
ion
14 www.origene.com
Cat. No. Accession No. Description
TC109629 NM_002757 Homo sapiens mitogen-activated protein kinase kinase 5 (MAP2K5), OriGene unique variant 1
TC124173 NM_002757 Homo sapiens mitogen-activated protein kinase kinase 5 (MAP2K5), transcript variant B
TC118432 NM_002758 Homo sapiens mitogen-activated protein kinase kinase 6 (MAP2K6), transcript variant 1
TC118433 NM_002759 Homo sapiens eukaryotic translation initiation factor 2-alpha kinase 2 (EIF2AK2)
TC118434 NM_002760 Homo sapiens protein kinase, Y-linked (PRKY)
TC118391 NM_002821 Homo sapiens PTK7 protein tyrosine kinase 7 (PTK7), transcript variant PTK7-1
TC109662 NM_002822 Homo sapiens PTK9 protein tyrosine kinase 9 (PTK9), transcript variant 1
TC109663 NM_002822 Homo sapiens PTK9 protein tyrosine kinase 9 (PTK9), OriGene unique variant 1
TC118322 NM_002880 Homo sapiens v-raf-1 murine leukemia viral oncogene homolog 1 (RAF1), OriGene unique variant 1
TC118323 NM_002880 Homo sapiens v-raf-1 murine leukemia viral oncogene homolog 1 (RAF1)
TC118278 NM_002929 Homo sapiens G protein-coupled receptor kinase 1 (GRK1)
TC118295 NM_002953 Homo sapiens ribosomal protein S6 kinase, 90kDa, polypeptide 1 (RPS6KA1), transcript variant 1
TC112721 NM_002958 Homo sapiens RYK receptor-like tyrosine kinase (RYK), transcript variant 2
TC118305 NM_002969 Homo sapiens mitogen-activated protein kinase 12 (MAPK12)
TC118306 NM_002969 Homo sapiens mitogen-activated protein kinase 12 (MAPK12), OriGene unique variant 1
TC118239 NM_003010 Homo sapiens mitogen-activated protein kinase kinase 4 (MAP2K4), OriGene unique variant 1
TC118240 NM_003010 Homo sapiens mitogen-activated protein kinase kinase 4 (MAP2K4)
TC118241 NM_003010 Homo sapiens mitogen-activated protein kinase kinase 4 (MAP2K4), OriGene unique variant 2
TC118243 NM_003010 Homo sapiens mitogen-activated protein kinase kinase 4 (MAP2K4), OriGene unique variant 3
TC118244 NM_003010 Homo sapiens mitogen-activated protein kinase kinase 4 (MAP2K4), OriGene unique variant 4
TC118183 NM_003137 Homo sapiens SFRS protein kinase 1 (SRPK1)
TC118137 NM_003157 Homo sapiens NIMA (never in mitosis gene a)-related kinase 4 (NEK4)
TC108474 NM_003159 Homo sapiens cyclin-dependent kinase-like 5 (CDKL5), OriGene unique variant 1
TC118139 NM_003160 Homo sapiens aurora kinase C (AURKC)
TC118140 NM_003161 Homo sapiens ribosomal protein S6 kinase, 70kDa, polypeptide 1 (RPS6KB1)
TC118141 NM_003161 Homo sapiens ribosomal protein S6 kinase, 70kDa, polypeptide 1 (RPS6KB1), OriGene unique variant 1
TC118115 NM_003177 Homo sapiens spleen tyrosine kinase (SYK)
TC118119 NM_003188 Homo sapiens mitogen-activated protein kinase kinase kinase 7 (MAP3K7), transcript variant A
TC118147 NM_003215 Homo sapiens tec protein tyrosine kinase (TEC)
TC118072 NM_003242 Homo sapiens transforming growth factor, beta receptor II (70/80kDa) (TGFBR2)
TC118032 NM_003318 Homo sapiens TTK protein kinase (TTK)
TC118033 NM_003318 Homo sapiens TTK protein kinase (TTK), OriGene unique variant 1
TC118038 NM_003328 Homo sapiens TXK tyrosine kinase (TXK)
TC111048 NM_003331 Homo sapiens tyrosine kinase 2 (TYK2)
TC117994 NM_003384 Homo sapiens vaccinia related kinase 1 (VRK1)
TC117999 NM_003390 Homo sapiens WEE1 homolog (S. pombe) (WEE1)
TC117908 NM_003503 Homo sapiens CDC7 cell division cycle 7 (S. cerevisiae) (CDC7)
TC117907 NM_003576 Homo sapiens serine/threonine kinase 24 (STE20 homolog, yeast) (STK24)
TC117867 NM_003582 Homo sapiens dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 3 (DYRK3), transcript
variant 1
TC109928 NM_003583 Homo sapiens dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 2 (DYRK2), transcript
variant 1
TC117877 NM_003600 Homo sapiens serine/threonine kinase 6 (STK6), transcript variant 2
TC117892 NM_003618 Homo sapiens mitogen-activated protein kinase kinase kinase kinase 3 (MAP4K3), OriGene unique
variant 1
TC117893 NM_003618 Homo sapiens mitogen-activated protein kinase kinase kinase kinase 3 (MAP4K3)
TC117843 NM_003656 Homo sapiens calcium/calmodulin-dependent protein kinase I (CAMK1)
TC117844 NM_003656 Homo sapiens calcium/calmodulin-dependent protein kinase I (CAMK1), OriGene unique variant 1
Tru
eClo
ne™
Clon
eSet
:Gen
e-fa
mily
base
dco
llect
ion
15ph: 1.888.267.4436 fax: 1.301.340.9254
Cat. No. Accession No. Description
TC109943 NM_003668 Homo sapiens mitogen-activated protein kinase-activated protein kinase 5 (MAPKAPK5), transcript
variant 1
TC109944 NM_003668 Homo sapiens mitogen-activated protein kinase-activated protein kinase 5 (MAPKAPK5), OriGene
unique variant 1
TC109951 NM_003674 Homo sapiens cyclin-dependent kinase (CDC2-like) 10 (CDK10), OriGene unique variant 1
TC109952 NM_003674 Homo sapiens cyclin-dependent kinase (CDC2-like) 10 (CDK10), transcript variant 1
TC117862 NM_003684 Homo sapiens MAP kinase interacting serine/threonine kinase 1 (MKNK1)
TC117810 NM_003688 Homo sapiens calcium/calmodulin-dependent serine protein kinase (MAGUK family) (CASK)
TC108468 NM_003691 Homo sapiens serine/threonine kinase 16 (STK16), transcript variant 1
TC109966 NM_003718 Homo sapiens cell division cycle 2-like 5 (cholinesterase-related cell division controller) (CDC2L5),
transcript variant 1
TC108466 NM_003804 Homo sapiens receptor (TNFRSF)-interacting serine-threonine kinase 1 (RIPK1)
TC117734 NM_003821 Homo sapiens receptor-interacting serine-threonine kinase 2 (RIPK2)
TC117735 NM_003821 Homo sapiens receptor-interacting serine-threonine kinase 2 (RIPK2), OriGene unique variant 1
TC109987 NM_003831 Homo sapiens RIO kinase 3 (yeast) (RIOK3), transcript variant 1
TC106951 NM_003845 Homo sapiens dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 4 (DYRK4), OriGene
unique variant 1
TC106952 NM_003845 Homo sapiens dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 4 (DYRK4)
TC117716 NM_003852 Homo sapiens transcriptional intermediary factor 1 (TIF1), transcript variant 2
TC107962 NM_003913 Homo sapiens PRP4 pre-mRNA processing factor 4 homolog B (yeast) (PRPF4B), transcript variant 1
TC117648 NM_003942 Homo sapiens ribosomal protein S6 kinase, 90kDa, polypeptide 4 (RPS6KA4), transcript variant 1
TC111095 NM_003948 Homo sapiens cyclin-dependent kinase-like 2 (CDC2-related kinase) (CDKL2)
TC117656 NM_003952 Homo sapiens ribosomal protein S6 kinase, 70kDa, polypeptide 2 (RPS6KB2), OriGene unique variant 1
TC124062 NM_003952 Homo sapiens ribosomal protein S6 kinase, 70kDa, polypeptide 2 (RPS6KB2), transcript variant 1
TC117658 NM_003954 Homo sapiens mitogen-activated protein kinase kinase kinase 14 (MAP3K14)
TC101068 NM_003957 Homo sapiens BR serine/threonine kinase 2 (BRSK2)
TC117643 NM_003985 Homo sapiens tyrosine kinase, non-receptor, 1 (TNK1)
TC109075 NM_003992 Homo sapiens CDC-like kinase 3 (CLK3), transcript variant phclk3
TC109076 NM_003992 Homo sapiens CDC-like kinase 3 (CLK3), OriGene unique variant 1hclk3
TC117645 NM_003993 Homo sapiens CDC-like kinase 2 (CLK2), transcript variant 1
TC117646 NM_003995 Homo sapiens natriuretic peptide receptor B/guanylate cyclase B (atrionatriuretic peptide receptor B) (NPR2)
TC117573 NM_004071 Homo sapiens CDC-like kinase 1 (CLK1), OriGene unique variant 1
TC117574 NM_004071 Homo sapiens CDC-like kinase 1 (CLK1)
TC117577 NM_004073 Homo sapiens polo-like kinase 3 (Drosophila) (PLK3)
TC109209 NM_004103 Homo sapiens PTK2B protein tyrosine kinase 2 beta (PTK2B), transcript variant 2
TC124086 NM_004119 Homo sapiens fms-related tyrosine kinase 3 (FLT3)
TC109998 NM_004197 Homo sapiens serine/threonine kinase 19 (STK19), transcript variant 1
TC109999 NM_004197 Homo sapiens serine/threonine kinase 19 (STK19), OriGene unique variant 1
TC117519 NM_004203 Homo sapiens membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase
(PKMYT1), transcript variant 1
TC117533 NM_004226 Homo sapiens serine/threonine kinase 17b (apoptosis-inducing) (STK17B)
TC108895 NM_004302 Homo sapiens activin A receptor, type IB (ACVR1B), transcript variant 1
TC117431 NM_004327 Homo sapiens breakpoint cluster region (BCR), transcript variant 1
TC117433 NM_004329 Homo sapiens bone morphogenetic protein receptor, type IA (BMPR1A)
TC117437 NM_004333 Homo sapiens v-raf murine sarcoma viral oncogene homolog B1 (BRAF)
TC110841 NM_004336 Homo sapiens BUB1 budding uninhibited by benzimidazoles 1 homolog (yeast) (BUB1)
TC117389 NM_004383 Homo sapiens c-src tyrosine kinase (CSK)
TC108512 NM_004384 Homo sapiens casein kinase 1, gamma 3 (CSNK1G3)
TC117404 NM_004409 Homo sapiens dystrophia myotonica-protein kinase (DMPK)
Tru
eClo
ne™
Clon
eSet
:Gen
e-fa
mily
base
dco
llect
ion
16 www.origene.com
Cat. No. Accession No. Description
TC117346 NM_004431 Homo sapiens EPH receptor A2 (EPHA2)
TC121913 NM_004431 Homo sapiens EPH receptor A2 (EPHA2)
TC117354 NM_004438 Homo sapiens EPH receptor A4 (EPHA4)
TC111626 NM_004439 Homo sapiens EPH receptor A5 (EPHA5), transcript variant 1
TC108415 NM_004440 Homo sapiens EPH receptor A7 (EPHA7)
TC117355 NM_004441 Homo sapiens EPH receptor B1 (EPHB1)
TC117356 NM_004443 Homo sapiens EPH receptor B3 (EPHB3)
TC117357 NM_004444 Homo sapiens EPH receptor B4 (EPHB4)
TC117358 NM_004445 Homo sapiens EPH receptor B6 (EPHB6)
TC125233 NM_004448 Homo sapiens v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived
oncogene homolog (avian) (ERBB2), transcript variant 1
TC117313 NM_004517 Homo sapiens integrin-linked kinase (ILK)
TC117279 NM_004560 Homo sapiens receptor tyrosine kinase-like orphan receptor 2 (ROR2)
TC117289 NM_004579 Homo sapiens mitogen-activated protein kinase kinase kinase kinase 2 (MAP4K2)
TC111005 NM_004586 Homo sapiens ribosomal protein S6 kinase, 90kDa, polypeptide 3 (RPS6KA3), OriGene unique variant 1
TC111006 NM_004586 Homo sapiens ribosomal protein S6 kinase, 90kDa, polypeptide 3 (RPS6KA3)
TC108520 NM_004612 Homo sapiens transforming growth factor, beta receptor I (activin A receptor type II-like kinase, 53kDa)
(TGFBR1)
TC117238 NM_004635 Homo sapiens mitogen-activated protein kinase-activated protein kinase 3 (MAPKAPK3)
TC111727 NM_004672 Homo sapiens mitogen-activated protein kinase kinase kinase 6 (MAP3K6)
TC117200 NM_004690 Homo sapiens LATS, large tumor suppressor, homolog 1 (Drosophila) (LATS1)”
TC117223 NM_004714 Homo sapiens dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1B (DYRK1B), transcript
variant a
TC117227 NM_004721 Homo sapiens mitogen-activated protein kinase kinase kinase 13 (MAP3K13)
TC108525 NM_004734 Homo sapiens doublecortin and CaM kinase-like 1 (DCAMKL1)
TC117159 NM_004759 Homo sapiens mitogen-activated protein kinase-activated protein kinase 2 (MAPKAPK2), transcript
variant 1
TC117160 NM_004760 Homo sapiens serine/threonine kinase 17a (apoptosis-inducing) (STK17A)
TC117141 NM_004783 Homo sapiens TAO kinase 2 (TAOK2)
TC117132 NM_004836 Homo sapiens eukaryotic translation initiation factor 2-alpha kinase 3 (EIF2AK3)
TC117098 NM_004850 Homo sapiens Rho-associated, coiled-coil containing protein kinase 2 (ROCK2)
TC117039 NM_004935 Homo sapiens cyclin-dependent kinase 5 (CDK5)
TC110866 NM_004938 Homo sapiens death-associated protein kinase 1 (DAPK1)
TC110899 NM_004958 Homo sapiens FK506 binding protein 12-rapamycin associated protein 1 (FRAP1)
TC117052 NM_004963 Homo sapiens guanylate cyclase 2C (heat stable enterotoxin receptor) (GUCY2C)
TC117059 NM_004972 Homo sapiens Janus kinase 2 (a protein tyrosine kinase) (JAK2)
TC117005 NM_005012 Homo sapiens receptor tyrosine kinase-like orphan receptor 1 (ROR1)
TC110978 NM_005030 Homo sapiens polo-like kinase 1 (Drosophila) (PLK1)
TC116959 NM_005044 Homo sapiens protein kinase, X-linked (PRKX)
TC116928 NM_005104 Homo sapiens bromodomain containing 2 (BRD2)
TC111128 NM_005109 Homo sapiens oxidative-stress responsive 1 (OXSR1)
TC116914 NM_005157 Homo sapiens v-abl Abelson murine leukemia viral oncogene homolog 1 (ABL1), transcript variant a
TC116916 NM_005160 Homo sapiens adrenergic, beta, receptor kinase 2 (ADRBK2)
TC116917 NM_005160 Homo sapiens adrenergic, beta, receptor kinase 2 (ADRBK2), OriGene unique variant 1
TC116883 NM_005163 Homo sapiens v-akt murine thymoma viral oncogene homolog 1 (AKT1)
TC112674 NM_005204 Homo sapiens mitogen-activated protein kinase kinase kinase 8 (MAP3K8)
TC116882 NM_005211 Homo sapiens colony stimulating factor 1 receptor, formerly McDonough feline sarcoma viral (v-fms)
oncogene homolog (CSF1R)
TC110878 NM_005228 Homo sapiens epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene
homolog, avian) (EGFR), transcript variant 1
Tru
eClo
ne™
Clon
eSet
:Gen
e-fa
mily
base
dco
llect
ion
17ph: 1.888.267.4436 fax: 1.301.340.9254
Cat. No. Accession No. Description
TC116861 NM_005232 Homo sapiens EPH receptor A1 (EPHA1)
TC109197 NM_005233 Homo sapiens EPH receptor A3 (EPHA3), transcript variant 1
TC116863 NM_005235 Homo sapiens v-erb-a erythroblastic leukemia viral oncogene homolog 4 (avian) (ERBB4), OriGene
unique variant 1
TC116869 NM_005246 Homo sapiens fer (fps/fes related) tyrosine kinase (phosphoprotein NCP94) (FER)
TC116870 NM_005248 Homo sapiens Gardner-Rasheed feline sarcoma viral (v-fgr) oncogene homolog (FGR)
TC116876 NM_005255 Homo sapiens cyclin G associated kinase (GAK)
TC116815 NM_005307 Homo sapiens G protein-coupled receptor kinase 4 (GRK4), OriGene unique variant 1
TC110910 NM_005308 Homo sapiens G protein-coupled receptor kinase 5 (GRK5)
TC116769 NM_005356 Homo sapiens lymphocyte-specific protein tyrosine kinase (LCK), OriGene unique variant 1
TC116770 NM_005356 Homo sapiens lymphocyte-specific protein tyrosine kinase (LCK)
TC110971 NM_005391 Homo sapiens pyruvate dehydrogenase kinase, isoenzyme 3 (PDK3)
TC116792 NM_005400 Homo sapiens protein kinase C, epsilon (PRKCE)
TC116752 NM_005417 Homo sapiens v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) (SRC), transcript
variant 1
TC116728 NM_005424 Homo sapiens tyrosine kinase with immunoglobulin-like and EGF-like domains 1 (TIE1)
TC116734 NM_005433 Homo sapiens v-yes-1 Yamaguchi sarcoma viral oncogene homolog 1 (YES1)
TC110105 NM_005465 Homo sapiens v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma) (AKT3),
transcript variant 1
TC116679 NM_005546 Homo sapiens IL2-inducible T-cell kinase (ITK)
TC116659 NM_005569 Homo sapiens LIM domain kinase 2 (LIMK2), transcript variant 2a
TC116680 NM_005592 Homo sapiens muscle, skeletal, receptor tyrosine kinase (MUSK)
TC116628 NM_005607 Homo sapiens PTK2 protein tyrosine kinase 2 (PTK2), transcript variant 2
TC116597 NM_005627 Homo sapiens serum/glucocorticoid regulated kinase (SGK)
TC116599 NM_005627 Homo sapiens serum/glucocorticoid regulated kinase (SGK), OriGene unique variant 1
TC124179 NM_005734 Homo sapiens homeodomain interacting protein kinase 3 (HIPK3)
TC116515 NM_005762 Homo sapiens tripartite motif-containing 28 (TRIM28)
TC116528 NM_005781 Homo sapiens tyrosine kinase, non-receptor, 2 (TNK2), transcript variant 1
TC116506 NM_005813 Homo sapiens protein kinase D3 (PRKD3)
TC116464 NM_005881 Homo sapiens branched chain ketoacid dehydrogenase kinase (BCKDK)
TC116439 NM_005906 Homo sapiens male germ cell-associated kinase (MAK)
TC116450 NM_005923 Homo sapiens mitogen-activated protein kinase kinase kinase 5 (MAP3K5)
TC116404 NM_005975 Homo sapiens PTK6 protein tyrosine kinase 6 (PTK6)
TC124087 NM_005975 Homo sapiens PTK6 protein tyrosine kinase 6 (PTK6)
TC116411 NM_005990 Homo sapiens serine/threonine kinase 10 (STK10)
TC116371 NM_006035 Homo sapiens CDC42 binding protein kinase beta (DMPK-like) (CDC42BPB)
TC116294 NM_006182 Homo sapiens discoidin domain receptor family, member 2 (DDR2)
TC111664 NM_006201 Homo sapiens PCTAIRE protein kinase 1 (PCTK1), transcript variant 1
TC116256 NM_006206 Homo sapiens platelet-derived growth factor receptor, alpha polypeptide (PDGFRA)
TC116261 NM_006213 Homo sapiens phosphorylase kinase, gamma 1 (muscle) (PHKG1)
TC116246 NM_006251 Homo sapiens protein kinase, AMP-activated, alpha 1 catalytic subunit (PRKAA1), transcript variant 1
TC116247 NM_006252 Homo sapiens protein kinase, AMP-activated, alpha 2 catalytic subunit (PRKAA2)
TC116249 NM_006254 Homo sapiens protein kinase C, delta (PRKCD), transcript variant 1
TC116250 NM_006255 Homo sapiens protein kinase C, eta (PRKCH)
TC108568 NM_006256 Homo sapiens protein kinase N2 (PKN2)
TC116251 NM_006257 Homo sapiens protein kinase C, theta (PRKCQ)
TC116252 NM_006258 Homo sapiens protein kinase, cGMP-dependent, type I (PRKG1)
TC116254 NM_006259 Homo sapiens protein kinase, cGMP-dependent, type II (PRKG2)
TC116188 NM_006281 Homo sapiens serine/threonine kinase 3 (STE20 homolog, yeast) (STK3)
Tru
eClo
ne™
Clon
eSet
:Gen
e-fa
mily
base
dco
llect
ion
18 www.origene.com
Cat. No. Accession No. Description
TC116189 NM_006282 Homo sapiens serine/threonine kinase 4 (STK4)
TC116192 NM_006285 Homo sapiens testis-specific kinase 1 (TESK1)
TC108283 NM_006293 Homo sapiens TYRO3 protein tyrosine kinase (TYRO3)
TC116202 NM_006296 Homo sapiens vaccinia related kinase 2 (VRK2)
TC116203 NM_006296 Homo sapiens vaccinia related kinase 2 (VRK2), OriGene unique variant 1
TC116209 NM_006301 Homo sapiens mitogen-activated protein kinase kinase kinase 12 (MAP3K12), OriGene unique variant 1
TC116132 NM_006343 Homo sapiens c-mer proto-oncogene tyrosine kinase (MERTK)
TC111155 NM_006374 Homo sapiens serine/threonine kinase 25 (STE20 homolog, yeast) (STK25)
TC111156 NM_006374 Homo sapiens serine/threonine kinase 25 (STE20 homolog, yeast) (STK25), OriGene unique variant 1
TC109929 NM_006482 Homo sapiens dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 2 (DYRK2), transcript
variant 2
TC124175 NM_006549 Homo sapiens calcium/calmodulin-dependent protein kinase kinase 2, beta (CAMKK2), transcript
variant 1
TC116033 NM_006549 Homo sapiens calcium/calmodulin-dependent protein kinase kinase 2, beta (CAMKK2), OriGene unique
variant 1
TC116034 NM_006549 Homo sapiens calcium/calmodulin-dependent protein kinase kinase 2, beta (CAMKK2), OriGene unique
variant 2
TC110237 NM_006575 Homo sapiens mitogen-activated protein kinase kinase kinase kinase 5 (MAP4K5), transcript variant 1
TC115980 NM_006609 Homo sapiens mitogen-activated protein kinase kinase kinase 2 (MAP3K2)
TC115988 NM_006622 Homo sapiens polo-like kinase 2 (Drosophila) (PLK2)
TC115926 NM_006712 Homo sapiens FAST kinase (FASTK), transcript variant 1
TC101252 NM_006742 Homo sapiens protein serine kinase H1 (PSKH1)
TC115810 NM_006852 Homo sapiens tousled-like kinase 2 (TLK2)
TC115811 NM_006852 Homo sapiens tousled-like kinase 2 (TLK2), OriGene unique variant 1
TC115812 NM_006852 Homo sapiens tousled-like kinase 2 (TLK2), OriGene unique variant 2
TC115825 NM_006875 Homo sapiens pim-2 oncogene (PIM2)
TC110233 NM_007079 Homo sapiens protein tyrosine phosphatase type IVA, member 3 (PTP4A3), transcript variant 2
TC115660 NM_007170 Homo sapiens testis-specific kinase 2 (TESK2)
TC115668 NM_007181 Homo sapiens mitogen-activated protein kinase kinase kinase kinase 1 (MAP4K1)
TC115669 NM_007181 Homo sapiens mitogen-activated protein kinase kinase kinase kinase 1 (MAP4K1), OriGene unique
variant 1
TC110240 NM_007194 Homo sapiens CHK2 checkpoint homolog (S. pombe) (CHEK2), transcript variant 1
TC115680 NM_007199 Homo sapiens interleukin-1 receptor-associated kinase 3 (IRAK3)
TC115629 NM_007271 Homo sapiens serine/threonine kinase 38 (STK38)
TC115604 NM_007284 Homo sapiens PTK9L protein tyrosine kinase 9-like (A6-related protein) (PTK9L)
TC115594 NM_007314 Homo sapiens v-abl Abelson murine leukemia viral oncogene homolog 2 (arg, Abelson-related gene)
(ABL2), transcript variant b
TC115567 NM_007371 Homo sapiens bromodomain containing 3 (BRD3)
TC111954 NM_012119 Homo sapiens cell cycle related kinase (CCRK), OriGene unique variant 1
TC124172 NM_012119 Homo sapiens cell cycle related kinase (CCRK), transcript variant 2
TC108453 NM_012290 Homo sapiens tousled-like kinase 1 (TLK1)
TC115413 NM_012395 Homo sapiens PFTAIRE protein kinase 1 (PFTK1), OriGene unique variant 1
TC115380 NM_012424 Homo sapiens ribosomal protein S6 kinase, 52kDa, polypeptide 1 (RPS6KC1)
TC115335 NM_013233 Homo sapiens serine threonine kinase 39 (STE20/SPS1 homolog, yeast) (STK39)
TC111251 NM_013254 Homo sapiens TANK-binding kinase 1 (TBK1)
TC115312 NM_013257 Homo sapiens serum/glucocorticoid regulated kinase-like (SGKL), transcript variant 1
TC108619 NM_013355 Homo sapiens protein kinase N3 (PKN3)
TC115246 NM_013392 Homo sapiens nuclear receptor binding protein (NRBP)
TC115206 NM_014002 Homo sapiens inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase epsilon (IKBKE)
Tru
eClo
ne™
Clon
eSet
:Gen
e-fa
mily
base
dco
llect
ion
19ph: 1.888.267.4436 fax: 1.301.340.9254
Cat. No. Accession No. Description
TC107297 NM_014215 Homo sapiens insulin receptor-related receptor (INSRR)
TC115138 NM_014226 Homo sapiens renal tumor antigen (RAGE), OriGene unique variant 1
TC115139 NM_014226 Homo sapiens renal tumor antigen (RAGE)
TC115082 NM_014264 Homo sapiens polo-like kinase 4 (Drosophila) (PLK4)
TC115101 NM_014299 Homo sapiens bromodomain containing 4 (BRD4), transcript variant short
TC115043 NM_014326 Homo sapiens death-associated protein kinase 2 (DAPK2)
TC115063 NM_014365 Homo sapiens heat shock 22kDa protein 8 (HSPB8)
TC115068 NM_014370 Homo sapiens serine/threonine kinase 23 (STK23)
TC124124 NM_014370 Homo sapiens serine/threonine kinase 23 (STK23)
TC124131 NM_014370 Homo sapiens serine/threonine kinase 23 (STK23)
TC115003 NM_014397 Homo sapiens NIMA (never in mitosis gene a)-related kinase 6 (NEK6)
TC115013 NM_014413 Homo sapiens eukaryotic translation initiation factor 2-alpha kinase 1 (EIF2AK1)
TC114987 NM_014496 Homo sapiens ribosomal protein S6 kinase, 90kDa, polypeptide 6 (RPS6KA6)
TC120535 NM_014572 Homo sapiens LATS, large tumor suppressor, homolog 2 (Drosophila) (LATS2)
TC100382 NM_014602 Homo sapiens phosphoinositide-3-kinase, regulatory subunit 4, p150 (PIK3R4)
TC114908 NM_014683 Homo sapiens unc-51-like kinase 2 (C. elegans) (ULK2)
TC114874 NM_014720 Homo sapiens STE20-like kinase (yeast) (SLK)
TC114824 NM_014791 Homo sapiens maternal embryonic leucine zipper kinase (MELK)
TC114791 NM_014826 Homo sapiens CDC42 binding protein kinase alpha (DMPK-like) (CDC42BPA), transcript variant A
TC114801 NM_014840 Homo sapiens AMP-activated protein kinase family member 5 (ARK5)
TC100957 NM_015000 Homo sapiens serine/threonine kinase 38 like (STK38L)
TC111200 NM_015148 Homo sapiens PAS domain containing serine/threonine kinase (PASK)
TC101100 NM_015690 Homo sapiens serine/threonine kinase 36 (fused homolog, Drosophila) (STK36)
TC114621 NM_015716 Homo sapiens misshapen-like kinase 1 (zebrafish) (MINK1), transcript variant 1
TC114629 NM_015850 Homo sapiens fibroblast growth factor receptor 1 (fms-related tyrosine kinase 2, Pfeiffer syndrome)
(FGFR1), transcript variant 2
TC114584 NM_015906 Homo sapiens tripartite motif-containing 33 (TRIM33), transcript variant alpha
TC114557 NM_015978 Homo sapiens TNNI3 interacting kinase (TNNI3K)
TC111598 NM_015981 Homo sapiens calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha (CAMK2A), transcript
variant 1
TC114467 NM_016123 Homo sapiens interleukin-1 receptor-associated kinase 4 (IRAK4), OriGene unique variant 1
TC114468 NM_016123 Homo sapiens interleukin-1 receptor-associated kinase 4 (IRAK4)
TC114352 NM_016231 Homo sapiens nemo like kinase (NLK)
TC114377 NM_016281 Homo sapiens TAO kinase 3 (TAOK3)
TC114263 NM_016440 Homo sapiens vaccinia related kinase 3 (VRK3)
TC114264 NM_016440 Homo sapiens vaccinia related kinase 3 (VRK3), OriGene unique variant 1
TC114274 NM_016457 Homo sapiens protein kinase D2 (PRKD2), OriGene unique variant 1
TC114275 NM_016457 Homo sapiens protein kinase D2 (PRKD2)
TC110272 NM_016513 Homo sapiens intestinal cell (MAK-like) kinase (ICK), transcript variant 2
TC114204 NM_016542 Homo sapiens Mst3 and SOK1-related kinase (MST4)
TC114182 NM_016653 Homo sapiens sterile alpha motif and leucine zipper containing kinase AZK (ZAK)
TC114149 NM_016733 Homo sapiens LIM domain kinase 2 (LIMK2), transcript variant 2b
TC109198 NM_017449 Homo sapiens EPH receptor B2 (EPHB2), transcript variant 1
TC114124 NM_017490 Homo sapiens MAP/microtubule affinity-regulating kinase 2 (MARK2), transcript variant 1
TC114079 NM_017572 Homo sapiens MAP kinase interacting serine/threonine kinase 2 (MKNK2)
TC110519 NM_017593 Homo sapiens BMP2 inducible kinase (BMP2K), transcript variant 2
TC114000 NM_017719 Homo sapiens SNF-1 related kinase (SNRK)
TC100365 NM_017771 Homo sapiens PX domain containing serine/threonine kinase (PXK)
Tru
eClo
ne™
Clon
eSet
:Gen
e-fa
mily
base
dco
llect
ion
20 www.origene.com
Cat. No. Accession No. Description
TC108694 NM_017886 Homo sapiens hypothetical protein FLJ20574 (FLJ20574)
TC113782 NM_017988 Homo sapiens SCY1-like 2 (S. cerevisiae) (SCYL2)
TC113560 NM_018343 Homo sapiens RIO kinase 2 (yeast) (RIOK2)
TC113491 NM_018401 Homo sapiens serine/threonine kinase 32B (STK32B)
TC113492 NM_018401 Homo sapiens serine/threonine kinase 32B (STK32B), OriGene unique variant 1
TC113493 NM_018401 Homo sapiens serine/threonine kinase 32B (STK32B), OriGene unique variant 2
TC113504 NM_018423 Homo sapiens serine/threonine/tyrosine kinase 1 (STYK1), OriGene unique variant 1
TC113505 NM_018423 Homo sapiens serine/threonine/tyrosine kinase 1 (STYK1)
TC113506 NM_018423 Homo sapiens serine/threonine/tyrosine kinase 1 (STYK1), OriGene unique variant 2
TC113461 NM_018492 Homo sapiens T-LAK cell-originated protein kinase (TOPK)
TC113432 NM_018571 Homo sapiens amyotrophic lateral sclerosis 2 (juvenile) chromosome region, candidate 2 (ALS2CR2)
TC113408 NM_018650 Homo sapiens MAP/microtubule affinity-regulating kinase 1 (MARK1)
TC112599 NM_019884 Homo sapiens glycogen synthase kinase 3 alpha (GSK3A)
TC113203 NM_020168 Homo sapiens p21(CDKN1A)-activated kinase 6 (PAK6)
TC111363 NM_020247 Homo sapiens chaperone, ABC1 activity of bc1 complex like (S. pombe) (CABC1)
TC110589 NM_020341 Homo sapiens p21(CDKN1A)-activated kinase 7 (PAK7), transcript variant 1
TC108612 NM_020421 Homo sapiens aarF domain containing kinase 1 (ADCK1)
TC113079 NM_020423 Homo sapiens ezrin-binding partner PACE-1 (PACE-1), transcript variant 1
TC108424 NM_020439 Homo sapiens calcium/calmodulin-dependent protein kinase IG (CAMK1G)
TC113095 NM_020547 Homo sapiens anti-Mullerian hormone receptor, type II (AMHR2)
TC113100 NM_020639 Homo sapiens receptor-interacting serine-threonine kinase 4 (RIPK4), OriGene unique variant 1
TC113041 NM_020666 Homo sapiens CDC-like kinase 4 (CLK4), OriGene unique variant 1
TC113042 NM_020666 Homo sapiens CDC-like kinase 4 (CLK4)
TC113047 NM_020680 Homo sapiens SCY1-like 1 (S. cerevisiae) (SCYL1)
TC107055 NM_020922 Homo sapiens WNK lysine deficient protein kinase 3 (WNK3), transcript variant 1
TC112978 NM_021133 Homo sapiens ribonuclease L (2’,5’-oligoisoadenylate synthetase-dependent) (RNASEL)
TC112980 NM_021135 Homo sapiens ribosomal protein S6 kinase, 90kDa, polypeptide 2 (RPS6KA2), transcript variant 1
TC112991 NM_021158 Homo sapiens tribbles homolog 3 (Drosophila) (TRIB3)
TC111247 NM_021643 Homo sapiens tribbles homolog 2 (Drosophila) (TRIB2)
TC112559 NM_021913 Homo sapiens AXL receptor tyrosine kinase (AXL), transcript variant 1
TC112854 NM_022048 Homo sapiens casein kinase 1, gamma 1 (CSNK1G1)”
TC112477 NM_022740 Homo sapiens homeodomain interacting protein kinase 2 (HIPK2)
TC109238 NM_023029 Homo sapiens fibroblast growth factor receptor 2 (bacteria-expressed kinase, keratinocyte growth factor
receptor, craniofacial dysostosis 1, Crouzon syndrome, Pfeiffer syndrome, Jackson-Weiss syndrome)
(FGFR2), transcript variant 11
TC109228 NM_023106 Homo sapiens fibroblast growth factor receptor 1 (fms-related tyrosine kinase 2, Pfeiffer syndrome)
(FGFR1), transcript variant 4
TC112299 NM_024046 Homo sapiens hypothetical protein MGC8407 (MGC8407)
TC111981 NM_024800 Homo sapiens NIMA (never in mitosis gene a)- related kinase 11 (NEK11)
TC108116 NM_024876 Homo sapiens aarF domain containing kinase 4 (ADCK4)
TC102414 NM_025096 Homo sapiens FAST kinase (FASTK), transcript variant 3
TC111013 NM_025144 Homo sapiens alpha-kinase 1 (ALPK1), OriGene unique variant 1
TC111024 NM_025144 Homo sapiens alpha-kinase 1 (ALPK1)
TC110324 NM_025195 Homo sapiens tribbles homolog 1 (Drosophila) (TRIB1)
TC110003 NM_030662 Homo sapiens mitogen-activated protein kinase kinase 2 (MAP2K2)
TC100378 NM_030906 Homo sapiens serine/threonine kinase 33 (STK33)
TC109313 NM_030952 Homo sapiens likely ortholog of rat SNF1/AMP-activated protein kinase (SNARK)
TC107236 NM_031414 Homo sapiens serine/threonine kinase 31 (STK31), transcript variant 1
Tru
eClo
ne™
Clon
eSet
:Gen
e-fa
mily
base
dco
llect
ion
21ph: 1.888.267.4436 fax: 1.301.340.9254
Cat. No. Accession No. Description
TC107258 NM_031417 Homo sapiens MAP/microtubule affinity-regulating kinase 4
(MARK4)
TC107535 NM_031464 Homo sapiens ribosomal protein S6 kinase-like 1 (RPS6KL1)
TC110673 NM_031480 Homo sapiens RIO kinase 1 (yeast) (RIOK1), transcript variant 1
TC111461 NM_031965 Homo sapiens germ cell associated 2 (haspin) (GSG2)
TC107277 NM_032017 Homo sapiens Ser/Thr-like kinase (MGC4796)
TC106635 NM_032028 Homo sapiens serine/threonine kinase 22D (spermiogenesis associated) (STK22D)
TC106670 NM_032037 Homo sapiens serine/threonine protein kinase SSTK (SSTK)
TC105614 NM_032237 Homo sapiens hypothetical protein FLJ23356 (FLJ23356)
TC124141 NM_032237 Homo sapiens hypothetical protein FLJ23356 (FLJ23356)
TC108012 NM_032409 Homo sapiens PTEN induced putative kinase 1 (PINK1)
TC124174 NM_032454 Homo sapiens serine/threonine kinase 19 (STK19), transcript variant 2
TC103147 NM_032844 Homo sapiens microtubule associated serine/threonine kinase-like (MASTL)
TC101836 NM_033015 Homo sapiens FAST kinase (FASTK), transcript variant 4
TC109561 NM_033018 Homo sapiens PCTAIRE protein kinase 1 (PCTK1), transcript variant 2
TC101858 NM_033019 Homo sapiens PCTAIRE protein kinase 1 (PCTK1), OriGene unique variant 1
TC124171 NM_033020 Homo sapiens tripartite motif-containing 33 (TRIM33), transcript variant beta
TC111489 NM_033116 Homo sapiens NIMA (never in mitosis gene a)- related kinase 9 (NEK9)
TC101725 NM_033118 Homo sapiens myosin light chain kinase 2, skeletal muscle (MYLK2)
TC101381 NM_033266 Homo sapiens endoplasmic reticulum to nucleus signalling 2 (ERN2)
TC120376 NM_033550 Homo sapiens TP53 regulating kinase (TP53RK)
TC120138 NM_052841 Homo sapiens serine/threonine kinase 22C (spermiogenesis associated) (STK22C)
TC120143 NM_052853 Homo sapiens aarF domain containing kinase 2 (ADCK2)
TC120190 NM_053006 Homo sapiens serine/threonine kinase 22B (spermiogenesis associated) (STK22B)
TC109468 NM_053025 Homo sapiens myosin, light polypeptide kinase (MYLK), transcript variant 1
TC109471 NM_053030 Homo sapiens myosin, light polypeptide kinase (MYLK), transcript variant 5
TC120354 NM_080823 Homo sapiens src-related kinase lacking C-terminal regulatory tyrosine and N-terminal myristylation
sites (SRMS)
TC120556 NM_130436 Homo sapiens dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1A (DYRK1A), transcript
variant 2
TC120526 NM_133494 Homo sapiens NIMA (never in mitosis gene a)-related kinase 7 (NEK7)
TC109616 NM_138957 Homo sapiens mitogen-activated protein kinase 1 (MAPK1), transcript variant 2
TC109626 NM_138980 Homo sapiens mitogen-activated protein kinase 10 (MAPK10), transcript variant 3
TC109627 NM_138982 Homo sapiens mitogen-activated protein kinase 10 (MAPK10), transcript variant 2
TC111522 NM_138995 Homo sapiens myosin IIIB (MYO3B)
TC124170 NM_139014 Homo sapiens mitogen-activated protein kinase 14 (MAPK14), transcript variant 4
TC120584 NM_139033 Homo sapiens mitogen-activated protein kinase 7 (MAPK7), transcript variant 1
TC120589 NM_139049 Homo sapiens mitogen-activated protein kinase 8 (MAPK8), transcript variant 1
TC108197 NM_139062 Homo sapiens casein kinase 1, delta (CSNK1D), transcript variant 2
TC120591 NM_139158 Homo sapiens amyotrophic lateral sclerosis 2 (juvenile) chromosome region, candidate 7 (ALS2CR7)
TC100071 NM_139355 Homo sapiens megakaryocyte-associated tyrosine kinase (MATK), transcript variant 1
TC120750 NM_144610 Homo sapiens hypothetical protein FLJ25006 (FLJ25006)
TC111529 NM_144685 Homo sapiens homeodomain interacting protein kinase 4 (HIPK4)
TC100039 NM_145001 Homo sapiens serine/threonine kinase 32A (STK32A)
TC109628 NM_145109 Homo sapiens mitogen-activated protein kinase kinase 3 (MAP2K3), transcript variant B
TC109633 NM_145185 Homo sapiens mitogen-activated protein kinase kinase 7 (MAP2K7)
TC100186 NM_145259 Homo sapiens activin A receptor, type IC (ACVR1C), OriGene unique variant 1
TC120776 NM_145259 Homo sapiens activin A receptor, type IC (ACVR1C), OriGene unique variant 2
Tru
eClo
ne™
Clon
eSet
:Gen
e-fa
mily
base
dco
llect
ion
22 www.origene.com
Cat. No. Accession No. Description
TC110026 NM_145319 Homo sapiens mitogen-activated protein kinase kinase kinase 6 (MAP3K6), transcript variant 2
TC100191 NM_145331 Homo sapiens mitogen-activated protein kinase kinase kinase 7 (MAP3K7), transcript variant B
TC100241 NM_145686 Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant 2
TC100654 NM_152221 Homo sapiens casein kinase 1, epsilon (CSNK1E), transcript variant 1
TC108071 NM_152534 Homo sapiens hypothetical protein FLJ32685 (FLJ32685)
TC100617 NM_152619 Homo sapiens doublecortin and CaM kinase-like 2 (DCAMKL2)
TC108101 NM_152649 Homo sapiens hypothetical protein FLJ34389 (FLJ34389)
TC111874 NM_152696 Homo sapiens homeodomain interacting protein kinase 1 (HIPK1), transcript variant 2
TC100783 NM_152835 Homo sapiens PDLIM1 interacting kinase 1 like (PDIK1L)
TC100784 NM_152835 Homo sapiens PDLIM1 interacting kinase 1 like (PDIK1L), OriGene unique variant 1
TC100826 NM_153335 Homo sapiens protein kinase LYK5 (LYK5), transcript variant 3
TC100346 NM_153361 Homo sapiens hypothetical protein MGC42105 (MGC42105)
TC100851 NM_153498 Homo sapiens calcium/calmodulin-dependent protein kinase ID (CAMK1D), transcript variant 2
TC101236 NM_153831 Homo sapiens PTK2 protein tyrosine kinase 2 (PTK2), transcript variant 1
TC110120 NM_170693 Homo sapiens serum/glucocorticoid regulated kinase 2 (SGK2), transcript variant 1
TC110121 NM_170693 Homo sapiens serum/glucocorticoid regulated kinase 2 (SGK2), OriGene unique variant 1
TC109000 NM_171825 Homo sapiens calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha (CAMK2A),
transcript variant 2
TC109001 NM_171825 Homo sapiens calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha (CAMK2A), OriGene
unique variant 1
TC109004 NM_172078 Homo sapiens calcium/calmodulin-dependent protein kinase (CaM kinase) II beta (CAMK2B), transcript
variant 2
TC109006 NM_172081 Homo sapiens calcium/calmodulin-dependent protein kinase (CaM kinase) II beta (CAMK2B), transcript
variant 5
TC109009 NM_172115 Homo sapiens calcium/calmodulin-dependent protein kinase (CaM kinase) II delta (CAMK2D), transcript
variant 4
TC108250 NM_172127 Homo sapiens calcium/calmodulin-dependent protein kinase (CaM kinase) II delta (CAMK2D), transcript
variant 1
TC100733 NM_172170 Homo sapiens calcium/calmodulin-dependent protein kinase (CaM kinase) II gamma (CAMK2G), transcript
variant 3
TC100734 NM_172170 Homo sapiens calcium/calmodulin-dependent protein kinase (CaM kinase) II gamma (CAMK2G),
OriGene unique variant 1
TC100735 NM_172170 Homo sapiens calcium/calmodulin-dependent protein kinase (CaM kinase) II gamma (CAMK2G),
OriGene unique variant 2
TC100737 NM_172170 Homo sapiens calcium/calmodulin-dependent protein kinase (CaM kinase) II gamma (CAMK2G),
OriGene unique variant 3
TC109012 NM_172171 Homo sapiens calcium/calmodulin-dependent protein kinase (CaM kinase) II gamma (CAMK2G),
transcript variant 1
TC101272 NM_173354 Homo sapiens SNF1-like kinase (SNF1LK)
TC101117 NM_173575 Homo sapiens serine/threonine kinase 32C (STK32C)
TC101143 NM_173677 Homo sapiens hypothetical protein FLJ40852 (FLJ40852), OriGene unique variant 1
TC101296 NM_174922 Homo sapiens aarF domain containing kinase 5 (ADCK5)
TC101352 NM_175866 Homo sapiens U2AF homology motif (UHM) kinase 1 (UHMK1)
TC107027 NM_177559 Homo sapiens casein kinase 2, alpha 1 polypeptide (CSNK2A1), transcript variant 1
TC108394 NM_177560 Homo sapiens casein kinase 2, alpha 1 polypeptide (CSNK2A1), transcript variant 3
TC108395 NM_177560 Homo sapiens casein kinase 2, alpha 1 polypeptide (CSNK2A1), OriGene unique variant 1
TC107077 NM_177990 Homo sapiens p21(CDKN1A)-activated kinase 7 (PAK7), transcript variant 2
TC107143 NM_178170 Homo sapiens NIMA (never in mitosis gene a)- related kinase 8 (NEK8)
TC107239 NM_178564 Homo sapiens nuclear receptor binding protein 2 (NRBP2)
TC107542 NM_182691 Homo sapiens SFRS protein kinase 2 (SRPK2), transcript variant 2
TC107658 U12140 Human tyrosine kinase receptor p145TRK-B (TRK-B) mRNA, complete cds
Tru
eClo
ne™
Clon
eSet
:Gen
e-fa
mily
base
dco
llect
ion
23ph: 1.888.267.4436 fax: 1.301.340.9254
Cat. No. Accession No. Description
TC107115 XM_027237 Homo sapiens mitogen-activated protein kinase kinase kinase 9 (MAP3K9)
TC103895 XM_032034 Homo sapiens syntrophin associated serine/threonine kinase (SAST)
TC120042 XM_032491 Homo sapiens inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta (IKBKB),
OriGene unique variant 1
TC120053 XM_032491 Homo sapiens inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta (IKBKB),
OriGene unique variant 2
TC120031 XM_032491 Homo sapiens inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta (IKBKB)
TC104537 XM_038150 PREDICTED: Homo sapiens microtubule associated serine/threonine kinase 3 (MAST3)
TC107086 XM_038576 PREDICTED: Homo sapiens hypothetical protein BC007901 (LOC91461)
TC107120 XM_040819 Homo sapiens similar to myosin light chain kinase (MLCK) (LOC91807)
TC116431 XM_041314 Homo sapiens KIAA0781 protein (KIAA0781)
TC116731 XM_042066 PREDICTED: Homo sapiens mitogen-activated protein kinase kinase kinase 1 (MAP3K1)
TC105253 XM_044630 Homo sapiens DKFZP434C131 protein (DKFZP434C131)
TC113998 XM_047355 PREDICTED: Homo sapiens KIAA1765 protein (KIAA1765)
TC105033 XM_055766 Homo sapiens mitogen-activated protein kinase 3 (MAPK3)
TC107663 XM_065695 Homo sapiens hypothetical gene LOC130382 (LOC130382) mRNA
TC107090 XM_114973 PREDICTED: Homo sapiens EphA6 (EPHA6)
TC107118 XM_208846 Homo sapiens similar to p21-activated protein kinase I (LOC283806)
TC107128 XM_210040 Homo sapiens similar to MAP/ERK kinase kinase 5; apoptosis signal regulating kinase [Homo sapiens]
(LOC286417)
TC107121 XM_211325 Homo sapiens LOC284084 (LOC284084)
TC107122 XM_290778 Homo sapiens apoptosis-associated tyrosine kinase (AATK)
TC107123 XM_290796 Homo sapiens serine/threonine protein kinase TAO1 homolog (KIAA1361)
TC107082 XM_290898 Homo sapiens KIAA0472 protein (KIAA0472)
TC107093 XM_291107 Homo sapiens NIMA (never in mitosis gene a)-related kinase 1 (NEK1)
TC107099 XM_291304 Homo sapiens hypothetical protein MGC43306 (MGC43306)
GPCR CloneSetCat. No. Accession No. Description
TC105790 AF411117 Homo sapiens G protein-coupled receptor (GPR103) mRNA, complete cds
TC106281 BC016940 Homo sapiens, Similar to olfactory receptor, family 2, subfamily A, member 4, clone IMAGE:4424116
TC107900 ENST00000317625 Database:core Gene:ENSG00000181109 Clone:AC109341 Contig:AC109341.7.1.202761 Chr:11
Basepair:5712017 Status:pseudogene
TC116997 NM_000024 Homo sapiens adrenergic, beta-2-, receptor, surface (ADRB2)
TC117219 NM_000025 Homo sapiens adrenergic, beta-3-, receptor (ADRB3)
TC115220 NM_000054 Homo sapiens arginine vasopressin receptor 2 (nephrogenic diabetes insipidus) (AVPR2)
TC111113 NM_000115 Homo sapiens endothelin receptor type B (EDNRB), transcript variant 1
TC109246 NM_000145 Homo sapiens follicle stimulating hormone receptor (FSHR), transcript variant 1
TC120082 NM_000160 Homo sapiens glucagon receptor (GCGR)
TC110906 NM_000164 Homo sapiens gastric inhibitory polypeptide receptor (GIPR)
TC119966 NM_000316 Homo sapiens parathyroid hormone receptor 1 (PTHR1)
TC119936 NM_000369 Homo sapiens thyroid stimulating hormone receptor (TSHR)
TC119946 NM_000388 Homo sapiens calcium-sensing receptor (hypocalciuric hypercalcemia 1, severe neonatal
hyperparathyroidism) (CASR)
TC119911 NM_000406 Homo sapiens gonadotropin-releasing hormone receptor (GNRHR)
TC119816 NM_000524 Homo sapiens 5-hydroxytryptamine (serotonin) receptor 1A (HTR1A)
Tru
eClo
ne™
Clon
eSet
:Gen
e-fa
mily
base
dco
llect
ion
24 www.origene.com
Cat. No. Accession No. Description
TC119819 NM_000529 Homo sapiens melanocortin 2 receptor (adrenocorticotropic hormone) (MC2R)
TC110858 NM_000579 Homo sapiens chemokine (C-C motif) receptor 5 (CCR5)
TC119793 NM_000621 Homo sapiens 5-hydroxytryptamine (serotonin) receptor 2A (HTR2A)
TC119794 NM_000623 Homo sapiens bradykinin receptor B2 (BDKRB2)
TC119771 NM_000634 Homo sapiens interleukin 8 receptor, alpha (IL8RA)
TC109079 NM_000647 Homo sapiens chemokine (C-C motif) receptor 2 (CCR2), transcript variant A
TC109082 NM_000648 Homo sapiens chemokine (C-C motif) receptor 2 (CCR2), transcript variant B
TC119756 NM_000674 Homo sapiens adenosine A1 receptor (ADORA1)
TC119757 NM_000675 Homo sapiens adenosine A2a receptor (ADORA2A)
TC119758 NM_000676 Homo sapiens adenosine A2b receptor (ADORA2B)
TC119759 NM_000677 Homo sapiens adenosine A3 receptor (ADORA3)
TC119760 NM_000678 Homo sapiens adrenergic, alpha-1D-, receptor (ADRA1D)
TC110821 NM_000679 Homo sapiens adrenergic, alpha-1B-, receptor (ADRA1B)
TC108910 NM_000680 Homo sapiens adrenergic, alpha-1A-, receptor (ADRA1A), transcript variant 1
TC119761 NM_000681 Homo sapiens adrenergic, alpha-2A-, receptor (ADRA2A)
TC124044 NM_000682 Homo sapiens adrenergic, alpha-2B-, receptor (ADRA2B)
TC107904 NM_000682 Homo sapiens adrenergic, alpha-2B-, receptor (ADRA2B)
TC124067 NM_000683 Homo sapiens adrenergic, alpha-2C-, receptor (ADRA2C), OriGene unique variant 1
TC124091 NM_000683 Homo sapiens adrenergic, alpha-2C-, receptor (ADRA2C)
TC119765 NM_000684 Homo sapiens adrenergic, beta-1-, receptor (ADRB1), OriGene unique variant 1
TC108918 NM_000685 Homo sapiens angiotensin II receptor, type 1 (AGTR1), transcript variant 1
TC119766 NM_000686 Homo sapiens angiotensin II receptor, type 2 (AGTR2)
TC110836 NM_000706 Homo sapiens arginine vasopressin receptor 1A (AVPR1A)
TC119717 NM_000707 Homo sapiens arginine vasopressin receptor 1B (AVPR1B)
TC119719 NM_000710 Homo sapiens bradykinin receptor B1 (BDKRB1)
TC122572 NM_000710 Homo sapiens bradykinin receptor B1 (BDKRB1)
TC119735 NM_000738 Homo sapiens cholinergic receptor, muscarinic 1 (CHRM1)
TC110855 NM_000739 Homo sapiens cholinergic receptor, muscarinic 2 (CHRM2), transcript variant 4
TC119736 NM_000740 Homo sapiens cholinergic receptor, muscarinic 3 (CHRM3)
TC119737 NM_000741 Homo sapiens cholinergic receptor, muscarinic 4 (CHRM4)
TC119692 NM_000794 Homo sapiens dopamine receptor D1 (DRD1)
TC119693 NM_000795 Homo sapiens dopamine receptor D2 (DRD2), transcript variant 1
TC124065 NM_000797 Homo sapiens dopamine receptor D4 (DRD4), OriGene unique variant 1
TC124155 NM_000797 Homo sapiens dopamine receptor D4 (DRD4)
TC119696 NM_000798 Homo sapiens dopamine receptor D5 (DRD5)
TC119635 NM_000823 Homo sapiens growth hormone releasing hormone receptor (GHRHR)
TC119643 NM_000838 Homo sapiens glutamate receptor, metabotropic 1 (GRM1)
TC119644 NM_000839 Homo sapiens glutamate receptor, metabotropic 2 (GRM2)
TC119645 NM_000840 Homo sapiens glutamate receptor, metabotropic 3 (GRM3)
TC119647 NM_000841 Homo sapiens glutamate receptor, metabotropic 4 (GRM4)
TC119648 NM_000842 Homo sapiens glutamate receptor, metabotropic 5 (GRM5)
TC119649 NM_000844 Homo sapiens glutamate receptor, metabotropic 7 (GRM7), transcript variant 1
TC110911 NM_000845 Homo sapiens glutamate receptor, metabotropic 8 (GRM8)
TC119662 NM_000861 Homo sapiens histamine receptor H1 (HRH1)
TC119612 NM_000863 Homo sapiens 5-hydroxytryptamine (serotonin) receptor 1B (HTR1B)
TC119613 NM_000864 Homo sapiens 5-hydroxytryptamine (serotonin) receptor 1D (HTR1D)
TC119614 NM_000865 Homo sapiens 5-hydroxytryptamine (serotonin) receptor 1E (HTR1E)
TC108583 NM_000866 Homo sapiens 5-hydroxytryptamine (serotonin) receptor 1F (HTR1F)
Tru
eClo
ne™
Clon
eSet
:Gen
e-fa
mily
base
dco
llect
ion
25ph: 1.888.267.4436 fax: 1.301.340.9254
Cat. No. Accession No. Description
TC119615 NM_000868 Homo sapiens 5-hydroxytryptamine (serotonin) receptor 2C (HTR2C)
TC119616 NM_000870 Homo sapiens 5-hydroxytryptamine (serotonin) receptor 4 (HTR4), transcript variant b
TC119617 NM_000871 Homo sapiens 5-hydroxytryptamine (serotonin) receptor 6 (HTR6)
TC119618 NM_000872 Homo sapiens 5-hydroxytryptamine (serotonin) receptor 7 (adenylate cyclase-coupled) (HTR7),
transcript variant a
TC119604 NM_000909 Homo sapiens neuropeptide Y receptor Y1 (NPY1R)
TC119605 NM_000910 Homo sapiens neuropeptide Y receptor Y2 (NPY2R)
TC119606 NM_000912 Homo sapiens opioid receptor, kappa 1 (OPRK1)
TC109521 NM_000913 Homo sapiens opiate receptor-like 1 (OPRL1), transcript variant 2
TC119609 NM_000914 Homo sapiens opioid receptor, mu 1 (OPRM1), transcript variant MOR-1
TC119610 NM_000916 Homo sapiens oxytocin receptor (OXTR)
TC119557 NM_000952 Homo sapiens platelet-activating factor receptor (PTAFR)
TC101345 NM_000953 Homo sapiens prostaglandin D2 receptor (DP) (PTGDR)
TC119559 NM_000955 Homo sapiens prostaglandin E receptor 1 (subtype EP1), 42kDa (PTGER1)
TC119560 NM_000956 Homo sapiens prostaglandin E receptor 2 (subtype EP2), 53kDa (PTGER2)
TC111682 NM_000957 Homo sapiens prostaglandin E receptor 3 (subtype EP3) (PTGER3), OriGene unique variant 1
TC119561 NM_000958 Homo sapiens prostaglandin E receptor 4 (subtype EP4) (PTGER4)
TC119562 NM_000959 Homo sapiens prostaglandin F receptor (FP) (PTGFR)
TC119563 NM_000960 Homo sapiens prostaglandin I2 (prostacyclin) receptor (IP) (PTGIR)
TC119481 NM_001049 Homo sapiens somatostatin receptor 1 (SSTR1)
TC119446 NM_001050 Homo sapiens somatostatin receptor 2 (SSTR2)
TC119447 NM_001051 Homo sapiens somatostatin receptor 3 (SSTR3)
TC119449 NM_001052 Homo sapiens somatostatin receptor 4 (SSTR4)
TC119450 NM_001053 Homo sapiens somatostatin receptor 5 (SSTR5)
TC119454 NM_001057 Homo sapiens tachykinin receptor 2 (TACR2)
TC119455 NM_001058 Homo sapiens tachykinin receptor 1 (TACR1), transcript variant long
TC111034 NM_001060 Homo sapiens thromboxane A2 receptor (TBXA2R), transcript variant 2
TC119444 NM_001118 Homo sapiens adenylate cyclase activating polypeptide 1 (pituitary) receptor type I (ADCYAP1R1)
TC119287 NM_001295 Homo sapiens chemokine (C-C motif) receptor 1 (CCR1)
TC119288 NM_001296 Homo sapiens chemokine binding protein 2 (CCBP2)
TC119314 NM_001337 Homo sapiens chemokine (C-X3-C motif) receptor 1 (CX3CR1)
TC119262 NM_001400 Homo sapiens endothelial differentiation, sphingolipid G-protein-coupled receptor, 1 (EDG1)
TC119263 NM_001401 Homo sapiens endothelial differentiation, lysophosphatidic acid G-protein-coupled receptor, 2 (EDG2),
transcript variant 1
TC119229 NM_001462 Homo sapiens formyl peptide receptor-like 1 (FPRL1), transcript variant 1
TC119233 NM_001466 Homo sapiens frizzled homolog 2 (Drosophila) (FZD2)
TC109253 NM_001470 Homo sapiens gamma-aminobutyric acid (GABA) B receptor, 1 (GABBR1), transcript variant 1
TC119210 NM_001504 Homo sapiens chemokine (C-X-C motif) receptor 3 (CXCR3)
TC119211 NM_001505 Homo sapiens G protein-coupled receptor 30 (GPR30)
TC119212 NM_001506 Homo sapiens G protein-coupled receptor 32 (GPR32)
TC119213 NM_001508 Homo sapiens G protein-coupled receptor 39 (GPR39)
TC119185 NM_001525 Homo sapiens hypocretin (orexin) receptor 1 (HCRTR1)
TC119187 NM_001526 Homo sapiens hypocretin (orexin) receptor 2 (HCRTR2)
TC119177 NM_001557 Homo sapiens interleukin 8 receptor, beta (IL8RB)
TC119090 NM_001704 Homo sapiens brain-specific angiogenesis inhibitor 3 (BAI3)
TC119065 NM_001727 Homo sapiens bombesin-like receptor 3 (BRS3)
TC119072 NM_001736 Homo sapiens complement component 5 receptor 1 (C5a ligand) (C5R1)
TC119074 NM_001742 Homo sapiens calcitonin receptor (CALCR)
Tru
eClo
ne™
Clon
eSet
:Gen
e-fa
mily
base
dco
llect
ion
26 www.origene.com
Cat. No. Accession No. Description
TC109039 NM_001784 Homo sapiens CD97 antigen (CD97), OriGene unique variant 1
TC109040 NM_001784 Homo sapiens CD97 antigen (CD97), transcript variant 2
TC118980 NM_001837 Homo sapiens chemokine (C-C motif) receptor 3 (CCR3), transcript variant 1
TC118981 NM_001838 Homo sapiens chemokine (C-C motif) receptor 7 (CCR7)
TC118984 NM_001841 Homo sapiens cannabinoid receptor 2 (macrophage) (CNR2)
TC118901 NM_001957 Homo sapiens endothelin receptor type A (EDNRA)
TC118924 NM_001992 Homo sapiens coagulation factor II (thrombin) receptor (F2R)
TC118857 NM_002029 Homo sapiens formyl peptide receptor 1 (FPR1)
TC118858 NM_002030 Homo sapiens formyl peptide receptor-like 2 (FPRL2)
TC110900 NM_002036 Homo sapiens Duffy blood group (FY)
TC118878 NM_002062 Homo sapiens glucagon-like peptide 1 receptor (GLP1R)
TC124060 NM_002062 Homo sapiens glucagon-like peptide 1 receptor (GLP1R)
TC118704 NM_002377 Homo sapiens MAS1 oncogene (MAS1)
TC118656 NM_002386 Homo sapiens melanocortin 1 receptor (alpha melanocyte stimulating hormone receptor) (MC1R)
TC118568 NM_002511 Homo sapiens neuromedin B receptor (NMBR)
TC110963 NM_002531 Homo sapiens neurotensin receptor 1 (high affinity) (NTSR1)
TC118599 NM_002563 Homo sapiens purinergic receptor P2Y, G-protein coupled, 1 (P2RY1)
TC118600 NM_002565 Homo sapiens pyrimidinergic receptor P2Y, G-protein coupled, 4 (P2RY4)
TC118351 NM_002921 Homo sapiens retinal G protein coupled receptor (RGR), OriGene unique variant 1
TC118062 NM_003301 Homo sapiens thyrotropin-releasing hormone receptor (TRHR)
TC117992 NM_003382 Homo sapiens vasoactive intestinal peptide receptor 2 (VIPR2)
TC117951 NM_003467 Homo sapiens chemokine (C-X-C motif) receptor 4 (CXCR4), transcript variant 2
TC117952 NM_003468 Homo sapiens frizzled homolog 5 (Drosophila) (FZD5)
TC117963 NM_003485 Homo sapiens G protein-coupled receptor 68 (GPR68)
TC117910 NM_003505 Homo sapiens frizzled homolog 1 (Drosophila) (FZD1)
TC117911 NM_003506 Homo sapiens frizzled homolog 6 (Drosophila) (FZD6)
TC117912 NM_003508 Homo sapiens frizzled homolog 9 (Drosophila) (FZD9)
TC117880 NM_003608 Homo sapiens G protein-coupled receptor 65 (GPR65)
TC117889 NM_003614 Homo sapiens galanin receptor 3 (GALR3)
TC117852 NM_003667 Homo sapiens leucine-rich repeat-containing G protein-coupled receptor 5 (LGR5)
TC117757 NM_003775 Homo sapiens endothelial differentiation, G-protein-coupled receptor 6 (EDG6)
TC117689 NM_003876 Homo sapiens chromosome 17 open reading frame 35 (C17orf35)
TC117654 NM_003950 Homo sapiens coagulation factor II (thrombin) receptor-like 3 (F2RL3)
TC117661 NM_003965 Homo sapiens chemokine (C-C motif) receptor-like 2 (CCRL2)
TC117662 NM_003967 Homo sapiens putative neurotransmitter receptor (PNR)
TC117639 NM_003979 Homo sapiens G protein-coupled receptor, family C, group 5, member A (GPCR5A)
TC117601 NM_004054 Homo sapiens complement component 3a receptor 1 (C3AR1)
TC117575 NM_004072 Homo sapiens chemokine-like receptor 1 (CMKLR1)
TC117592 NM_004101 Homo sapiens coagulation factor II (thrombin) receptor-like 2 (F2RL2)
TC117536 NM_004122 Homo sapiens growth hormone secretagogue receptor (GHSR), transcript variant 1b
TC109533 NM_004154 Homo sapiens pyrimidinergic receptor P2Y, G-protein coupled, 6 (P2RY6), transcript variant 4
TC117532 NM_004224 Homo sapiens G protein-coupled receptor 50 (GPR50)
TC117485 NM_004230 Homo sapiens endothelial differentiation, sphingolipid G-protein-coupled receptor, 5 (EDG5)
TC111108 NM_004246 Homo sapiens glucagon-like peptide 2 receptor (GLP2R)
TC117497 NM_004248 Homo sapiens G protein-coupled receptor 10 (GPR10)
TC110860 NM_004382 Homo sapiens corticotropin releasing hormone receptor 1 (CRHR1)
TC117230 NM_004624 Homo sapiens vasoactive intestinal peptide receptor 1 (VIPR1), OriGene unique variant 1
TC124135 NM_004624 Homo sapiens vasoactive intestinal peptide receptor 1 (VIPR1)
Tru
eClo
ne™
Clon
eSet
:Gen
e-fa
mily
base
dco
llect
ion
27ph: 1.888.267.4436 fax: 1.301.340.9254
Cat. No. Accession No. Description
TC117226 NM_004720 Homo sapiens endothelial differentiation, lysophosphatidic acid G-protein-coupled receptor, 4 (EDG4)
TC117166 NM_004767 Homo sapiens G-protein coupled receptor 37 like 1 (GPR37L1)
TC117171 NM_004778 Homo sapiens G protein-coupled receptor 44 (GPR44)
TC117047 NM_004951 Homo sapiens Epstein-Barr virus induced gene 2 (lymphocyte-specific G protein-coupled receptor) (EBI2)
TC116961 NM_005048 Homo sapiens parathyroid hormone receptor 2 (PTHR2)
TC116918 NM_005161 Homo sapiens angiotensin II receptor-like 1 (AGTRL1)
TC116904 NM_005201 Homo sapiens chemokine (C-C motif) receptor 8 (CCR8)
TC116857 NM_005226 Homo sapiens endothelial differentiation, sphingolipid G-protein-coupled receptor, 3 (EDG3)
TC116867 NM_005242 Homo sapiens coagulation factor II (thrombin) receptor-like 1 (F2RL1)
TC116835 NM_005279 Homo sapiens G protein-coupled receptor 1 (GPR1)
TC116836 NM_005281 Homo sapiens G protein-coupled receptor 3 (GPR3)
TC116837 NM_005282 Homo sapiens G protein-coupled receptor 4 (GPR4)
TC116839 NM_005283 Homo sapiens chemokine (C motif) receptor 1 (XCR1)
TC116840 NM_005284 Homo sapiens G protein-coupled receptor 6 (GPR6)
TC116841 NM_005285 Homo sapiens G protein-coupled receptor 7 (GPR7)
TC116844 NM_005286 Homo sapiens G protein-coupled receptor 8 (GPR8)
TC116845 NM_005288 Homo sapiens G protein-coupled receptor 12 (GPR12)
TC116846 NM_005290 Homo sapiens G protein-coupled receptor 15 (GPR15)
TC116799 NM_005291 Homo sapiens G protein-coupled receptor 17 (GPR17)
TC102869 NM_005292 Homo sapiens G protein-coupled receptor 18 (GPR18)
TC116800 NM_005293 Homo sapiens G protein-coupled receptor 20 (GPR20)
TC110909 NM_005294 Homo sapiens G protein-coupled receptor 21 (GPR21)
TC116801 NM_005295 Homo sapiens G protein-coupled receptor 22 (GPR22)
TC116802 NM_005296 Homo sapiens G protein-coupled receptor 23 (GPR23)
TC116803 NM_005297 Homo sapiens G protein-coupled receptor 24 (GPR24)
TC116804 NM_005298 Homo sapiens G protein-coupled receptor 25 (GPR25)
TC116805 NM_005299 Homo sapiens G protein-coupled receptor 31 (GPR31)
TC116807 NM_005300 Homo sapiens G protein-coupled receptor 34 (GPR34)
TC116808 NM_005301 Homo sapiens G protein-coupled receptor 35 (GPR35)
TC112677 NM_005302 Homo sapiens G protein-coupled receptor 37 (endothelin receptor type B-like) (GPR37)
TC108542 NM_005303 Homo sapiens G protein-coupled receptor 40 (GPR40), OriGene unique variant 1
TC116810 NM_005304 Homo sapiens G protein-coupled receptor 41 (GPR41)
TC116812 NM_005305 Homo sapiens G protein-coupled receptor 42 (GPR42)
TC116813 NM_005306 Homo sapiens G protein-coupled receptor 43 (GPR43)
TC116819 NM_005314 Homo sapiens gastrin-releasing peptide receptor (GRPR)
TC116745 NM_005458 Homo sapiens G protein-coupled receptor 51 (GPR51)
TC116695 NM_005508 Homo sapiens chemokine (C-C motif) receptor 4 (CCR4)
TC116556 NM_005682 Homo sapiens G protein-coupled receptor 56 (GPR56), transcript variant 1
TC116557 NM_005683 Homo sapiens G protein-coupled receptor 55 (GPR55)
TC116558 NM_005684 Homo sapiens G protein-coupled receptor 52 (GPR52)
TC108549 NM_005756 Homo sapiens G protein-coupled receptor 64 (GPR64)
TC116518 NM_005767 Homo sapiens purinergic receptor P2Y, G-protein coupled, 5 (P2RY5)
TC116534 NM_005795 Homo sapiens calcitonin receptor-like (CALCRL)
TC111748 NM_005866 Homo sapiens opioid receptor, sigma 1 (OPRS1), transcript variant 1
TC116445 NM_005912 Homo sapiens melanocortin 4 receptor (MC4R)
TC116446 NM_005913 Homo sapiens melanocortin 5 receptor (MC5R)
TC116399 NM_005958 Homo sapiens melatonin receptor 1A (MTNR1A)
TC116403 NM_005972 Homo sapiens pancreatic polypeptide receptor 1 (PPYR1)
Tru
eClo
ne™
Clon
eSet
:Gen
e-fa
mily
base
dco
llect
ion
28 www.origene.com
Cat. No. Accession No. Description
TC116358 NM_006018 Homo sapiens G protein-coupled receptor 109B (GPR109B)
TC116382 NM_006056 Homo sapiens neuromedin U receptor 1 (NMUR1)
TC116313 NM_006086 Homo sapiens tubulin, beta 3 (TUBB3)
TC116271 NM_006143 Homo sapiens G protein-coupled receptor 19 (GPR19)
TC116290 NM_006174 Homo sapiens neuropeptide Y receptor Y5 (NPY5R)
TC116043 NM_006564 Homo sapiens chemokine (C-X-C motif) receptor 6 (CXCR6)
TC115965 NM_006639 Homo sapiens cysteinyl leukotriene receptor 1 (CYSLTR1)
TC115852 NM_006794 Homo sapiens G protein-coupled receptor 75 (GPR75)
TC115678 NM_007197 Homo sapiens frizzled homolog 10 (Drosophila) (FZD10)
TC115645 NM_007223 Homo sapiens putative G protein coupled receptor (GPR)
TC115646 NM_007227 Homo sapiens G protein-coupled receptor 45 (GPR45)
TC115624 NM_007264 Homo sapiens adrenomedullin receptor (ADMR)
TC108456 NM_007369 Homo sapiens G protein-coupled receptor 161 (GPR161)
TC115571 NM_009585 Homo sapiens angiotensin II receptor, type 1 (AGTR1), transcript variant 2
TC115522 NM_012125 Homo sapiens cholinergic receptor, muscarinic 5 (CHRM5)
TC115507 NM_012152 Homo sapiens endothelial differentiation, lysophosphatidic acid G-protein-coupled receptor, 7 (EDG7)
TC115479 NM_012193 Homo sapiens frizzled homolog 4 (Drosophila) (FZD4)
TC115441 NM_012302 Homo sapiens latrophilin 2 (LPHN2)
TC115397 NM_012344 Homo sapiens neurotensin receptor 2 (NTSR2)
TC115400 NM_012360 Homo sapiens olfactory receptor, family 1, subfamily F, member 1 (OR1F1)
TC115401 NM_012368 Homo sapiens olfactory receptor, family 2, subfamily C, member 1 (OR2C1)
TC115273 NM_013308 Homo sapiens G protein-coupled receptor 171 (GPR171)
TC115294 NM_013345 Homo sapiens G protein-coupled receptor 132 (GPR132)
TC115222 NM_013447 Homo sapiens egf-like module containing, mucin-like, hormone receptor-like 2 (EMR2), transcript variant 1
TC115039 NM_014322 Homo sapiens opsin 3 (encephalopsin, panopsin) (OPN3)
TC112625 NM_014373 Homo sapiens G protein-coupled receptor 160 (GPR160)
TC115018 NM_014430 Homo sapiens cell death-inducing DFFA-like effector b (CIDEB)
TC110395 NM_014499 Homo sapiens purinergic receptor P2Y, G-protein coupled, 10 (P2RY10), transcript variant 1
TC114958 NM_014626 Homo sapiens G protein-coupled receptor 58 (GPR58)
TC114730 NM_014879 Homo sapiens purinergic receptor P2Y, G-protein coupled, 14 (P2RY14)
TC108169 NM_015236 Homo sapiens latrophilin 3 (LPHN3)
TC111611 NM_016083 Homo sapiens cannabinoid receptor 1 (brain) (CNR1), transcript variant 1
TC114355 NM_016235 Homo sapiens G protein-coupled receptor, family C, group 5, member B (GPRC5B)
TC114323 NM_016334 Homo sapiens G protein-coupled receptor 89 (GPR89)
TC114203 NM_016540 Homo sapiens G protein-coupled receptor 83 (GPR83)
TC111283 NM_016557 Homo sapiens chemokine (C-C motif) receptor-like 1 (CCRL1), transcript variant 2
TC114218 NM_016568 Homo sapiens relaxin 3 receptor 1 (RLN3R1)
TC114153 NM_016602 Homo sapiens chemokine (C-C motif) receptor 10 (CCR10)
TC107940 NM_017412 Homo sapiens frizzled homolog 3 (Drosophila) (FZD3)
TC113833 NM_017986 Homo sapiens G protein-coupled receptor 172B (GPR172B)
TC113456 NM_018485 Homo sapiens G protein-coupled receptor 77 (GPR77)
TC111332 NM_018490 Homo sapiens leucine-rich repeat-containing G protein-coupled receptor 4 (LGR4)
TC113412 NM_018654 Homo sapiens G protein-coupled receptor, family C, group 5, member D (GPRC5D)
TC113346 NM_018949 Homo sapiens urotensin 2 receptor (UTS2R)
TC113275 NM_018969 Homo sapiens G-protein coupled receptor 173 (GPR173)
TC113277 NM_018970 Homo sapiens G protein-coupled receptor 85 (GPR85)
TC113278 NM_018971 Homo sapiens G protein-coupled receptor 27 (GPR27)
TC113233 NM_019839 Homo sapiens leukotriene B4 receptor 2 (LTB4R2)
Tru
eClo
ne™
Clon
eSet
:Gen
e-fa
mily
base
dco
llect
ion
29ph: 1.888.267.4436 fax: 1.301.340.9254
Cat. No. Accession No. Description
TC110388 NM_019858 Homo sapiens gene rich cluster, A gene (GRCA), OriGene unique variant 1-2
TC109327 NM_019860 Homo sapiens 5-hydroxytryptamine (serotonin) receptor 7 (adenylate cyclase-coupled) (HTR7),
transcript variant b
TC113222 NM_019888 Homo sapiens melanocortin 3 receptor (MC3R)
TC113202 NM_020167 Homo sapiens neuromedin U receptor 2 (NMUR2)
TC108695 NM_020370 Homo sapiens G protein-coupled receptor 84 (GPR84)
TC113111 NM_020377 Homo sapiens cysteinyl leukotriene receptor 2 (CYSLTR2)
TC113123 NM_020400 Homo sapiens G protein-coupled receptor 92 (GPR92)
TC101276 NM_020455 Homo sapiens G protein-coupled receptor 126 (GPR126)
TC113097 NM_020633 Homo sapiens vomeronasal 1 receptor 1 (VN1R1)
TC112901 NM_021624 Homo sapiens histamine receptor H4 (HRH4)
TC112908 NM_021634 Homo sapiens leucine-rich repeat-containing G protein-coupled receptor 7 (LGR7)
TC109254 NM_021903 Homo sapiens gamma-aminobutyric acid (GABA) B receptor, 1 (GABBR1), transcript variant 2
TC112876 NM_021904 Homo sapiens gamma-aminobutyric acid (GABA) B receptor, 1 (GABBR1), transcript variant 3
TC110534 NM_022036 Homo sapiens G protein-coupled receptor, family C, group 5, member C (GPRC5C), transcript variant 1
TC107967 NM_022049 Homo sapiens G-protein coupled receptor 88 (GPR88)
TC112783 NM_022159 Homo sapiens EGF, latrophilin and seven transmembrane domain containing 1 (ELTD1)
TC112521 NM_022304 Homo sapiens histamine receptor H2 (HRH2)
TC110612 NM_022788 Homo sapiens purinergic receptor P2Y, G-protein coupled, 12 (P2RY12), transcript variant 1
TC112338 NM_023914 Homo sapiens purinergic receptor P2Y, G-protein coupled, 13 (P2RY13), transcript variant 1
TC108305 NM_023915 Homo sapiens G protein-coupled receptor 87 (GPR87)
TC112321 NM_024012 Homo sapiens 5-hydroxytryptamine (serotonin) receptor 5A (HTR5A)
TC108833 NM_024531 Homo sapiens G protein-coupled receptor 172A (GPR172A)
TC108034 NM_030760 Homo sapiens endothelial differentiation, sphingolipid G-protein-coupled receptor, 8 (EDG8)
TC109424 NM_030774 Homo sapiens olfactory receptor, family 51, subfamily E, member 2 (OR51E2)
TC109480 NM_030784 Homo sapiens G protein-coupled receptor 63 (GPR63)
TC107947 NM_031200 Homo sapiens chemokine (C-C motif) receptor 9 (CCR9), transcript variant A
TC107635 NM_031409 Homo sapiens chemokine (C-C motif) receptor 6 (CCR6), transcript variant 2
TC106692 NM_031936 Homo sapiens G protein-coupled receptor 61 (GPR61)
TC104391 NM_032503 Homo sapiens G protein-coupled receptor 145 (GPR145)
TC104503 NM_032551 Homo sapiens G protein-coupled receptor 54 (GPR54)
TC104514 NM_032553 Homo sapiens G protein-coupled receptor 174 (GPR174)
TC104525 NM_032554 Homo sapiens G protein-coupled receptor 81 (GPR81)
TC104225 NM_032635 Homo sapiens seven transmembrane domain protein (NIFIE14)
TC103581 NM_032787 Homo sapiens G protein-coupled receptor 128 (GPR128)
TC108023 NM_033050 Homo sapiens succinate receptor 1 (SUCNR1)
TC123076 NM_033050 Homo sapiens succinate receptor 1 (SUCNR1)
TC124092 NM_033663 Homo sapiens dopamine receptor D3 (DRD3), transcript variant e
TC120211 NM_053278 Homo sapiens trace amine receptor 5 (TRAR5)
TC120214 NM_054021 Homo sapiens G protein-coupled receptor 101 (GPR101)
TC120221 NM_054030 Homo sapiens G protein-coupled receptor MRGX2 (MRGX2)
TC120223 NM_054031 Homo sapiens G protein-coupled receptor MRGX3 (MRGX3)
TC120224 NM_054032 Homo sapiens G protein-coupled receptor MRGX4 (MRGX4)
TC109187 NM_057159 Homo sapiens endothelial differentiation, lysophosphatidic acid G-protein-coupled receptor, 2 (EDG2),
transcript variant 2
TC120259 NM_078481 Homo sapiens CD97 antigen (CD97), transcript variant 1
TC120361 NM_080817 Homo sapiens G protein-coupled receptor 82 (GPR82)
TC120362 NM_080818 Homo sapiens oxoglutarate (alpha-ketoglutarate) receptor 1 (OXGR1)
Tru
eClo
ne™
Clon
eSet
:Gen
e-fa
mily
base
dco
llect
ion
30 www.origene.com
Cat. No. Accession No. Description
TC120365 NM_080819 Homo sapiens G protein-coupled receptor 78 (GPR78)
TC108414 NM_080865 Homo sapiens G protein-coupled receptor 62 (GPR62)
TC120694 NM_138327 Homo sapiens trace amine receptor 1 (TRAR1)
TC120462 NM_138445 Homo sapiens G protein-coupled receptor 146 (GPR146), OriGene unique variant 1
TC120613 NM_138964 Homo sapiens G protein-coupled receptor 73 (GPR73)
TC100222 NM_144773 Homo sapiens G protein-coupled receptor 73-like 1 (GPR73L1)
TC100046 NM_145015 Homo sapiens MAS-related GPR, member F (MRGPRF)
TC100284 NM_147199 Homo sapiens G protein-coupled receptor MRGX1 (MRGX1)
TC100553 NM_148962 Homo sapiens oxoeicosanoid (OXE) receptor 1 (OXER1)
TC100589 NM_152430 Homo sapiens olfactory receptor, family 51, subfamily E, member 1 (OR51E1)
TC100645 NM_152529 Homo sapiens G protein-coupled receptor 155 (GPR155)
TC100361 NM_153442 Homo sapiens G protein-coupled receptor 26 (GPR26)
TC100531 NM_153832 Homo sapiens G protein-coupled receptor 161 (GPR161)
TC100887 NM_153837 Homo sapiens G protein-coupled receptor 114 (GPR114)
TC100901 NM_170776 Homo sapiens G protein-coupled receptor 97 (GPR97)
TC100902 NM_170776 Homo sapiens G protein-coupled receptor 97 (GPR97), OriGene unique variant 1
TC101264 NM_175057 Homo sapiens trace amine receptor 3 (TRAR3)
TC101263 NM_175067 Homo sapiens trace amine receptor 4 (TRAR4)
TC109532 NM_176072 Homo sapiens purinergic receptor P2Y, G-protein coupled, 2 (P2RY2), transcript variant 1
TC106974 NM_176875 Homo sapiens cholecystokinin B receptor (CCKBR)
TC107139 NM_178129 Homo sapiens purinergic receptor P2Y, G-protein coupled, 8 (P2RY8)
TC107310 NM_181657 Homo sapiens leukotriene B4 receptor (LTB4R)
TC107787 XM_049496 Homo sapiens purinergic receptor P2Y G-protein coupled 11 (P2RY11) mRNA
TC112665 XM_051522 Homo sapiens G protein-coupled receptor (RDC1)
TC107081 XM_060958 Homo sapiens similar to seven transmembrane helix receptor (LOC128373)
TC107898 XM_061555 Homo sapiens similar to G protein-coupled receptor 26 (LOC119586)
TC107103 XM_061618 Homo sapiens similar to seven transmembrane helix receptor (LOC119686)
TC107104 XM_062285 Homo sapiens similar to Olfactory receptor 2D3 (LOC120775)
TC107127 XM_066873 Homo sapiens G-protein coupled receptor 2 (GPCR2)
TC104550 XM_071093 Homo sapiens similar to Olfactory receptor 13C5 (LOC138799)
TC107105 XM_084536 Homo sapiens similar to seven transmembrane helix receptor (LOC143497)
TC107106 XM_089863 Homo sapiens similar to Olfactory receptor 52I2 (LOC143502)
TC107788 XM_089955 Homo sapiens similar to putative G-protein coupled receptor (LOC159948)
TC107116 XM_096782 Homo sapiens putative leukocyte platelet-activating factor receptor (HUMNPIIY20)
TC107107 XM_210196 Homo sapiens similar to seven transmembrane helix receptor (LOC283193), OriGene unique variant 1
TC107094 XM_291111 PREDICTED: Homo sapiens G protein-coupled receptor 125 (GPR125)
TC107084 XM_291419 Homo sapiens G protein-coupled receptor 153 (GPR153)
TC107108 XM_291874 Homo sapiens similar to seven transmembrane helix receptor (LOC341130)
TC107109 XM_291977 Homo sapiens similar to Olfactory receptor 52L1 (LOC338751)
TC107089 XM_293092 Homo sapiens G protein-coupled receptor 148 (GPR148)
TC107092 XM_293580 PREDICTED: Homo sapiens G protein-coupled receptor 149 (GPR149)
TC107098 XM_294318 Homo sapiens similar to Olfactory receptor 2A1 (LOC346528)
TC107899 XM_352008 Homo sapiens similar to seven transmembrane helix receptor (LOC375963)
TC107789 XM_352045 Homo sapiens similar to putative G-protein coupled receptor (LOC376035)
Tru
eClo
ne™
Clon
eSet
:Gen
e-fa
mily
base
dco
llect
ion
31ph: 1.888.267.4436 fax: 1.301.340.9254
Cat. No. Accession No. Description
TC114220 NM_000044 Homo sapiens androgen receptor (dihydrotestosterone receptor; testicular feminization; spinal and
bulbar muscular atrophy; Kennedy disease) (AR)
TC123890 NM_000125 Homo sapiens estrogen receptor 1 (ESR1)
TC123956 NM_000125 Homo sapiens estrogen receptor 1 (ESR1)
TC120774 NM_000125 Homo sapiens estrogen receptor 1 (ESR1)
TC120032 NM_000176 Homo sapiens nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor) (NR3C1)
TC119941 NM_000376 Homo sapiens vitamin D (1,25- dihydroxyvitamin D3) receptor (VDR)
TC123863 NM_000457 Homo sapiens hepatocyte nuclear factor 4, alpha (HNF4A), transcript variant 2
TC119844 NM_000461 Homo sapiens thyroid hormone receptor, beta (erythroblastic leukemia viral (v-erb-a) oncogene
homolog 2, avian) (THRB)
TC119849 NM_000475 Homo sapiens nuclear receptor subfamily 0, group B, member 1 (NR0B1)
TC123888 NM_000901 Homo sapiens nuclear receptor subfamily 3, group C, member 2 (NR3C2)
TC123962 NM_000926 Homo sapiens progesterone receptor (PGR)
TC119566 NM_000964 Homo sapiens retinoic acid receptor, alpha (RARA)
TC119567 NM_000965 Homo sapiens retinoic acid receptor, beta (RARB), transcript variant 1
TC119569 NM_000966 Homo sapiens retinoic acid receptor, gamma (RARG)
TC119215 NM_001437 Homo sapiens estrogen receptor 2 (ER beta) (ESR2), OriGene unique variant 1
TC119216 NM_001437 Homo sapiens estrogen receptor 2 (ER beta) (ESR2)
TC119217 NM_001438 Homo sapiens estrogen-related receptor gamma (ESRRG), transcript variant 1
TC119159 NM_001621 Homo sapiens aryl hydrocarbon receptor (AHR)
TC118806 NM_002135 Homo sapiens nuclear receptor subfamily 4, group A, member 1 (NR4A1), transcript variant 1
TC118329 NM_002889 Homo sapiens retinoic acid receptor responder (tazarotene induced) 2 (RARRES2)
TC109741 NM_002943 Homo sapiens RAR-related orphan receptor A (RORA), transcript variant 3
TC109742 NM_002943 Homo sapiens RAR-related orphan receptor A (RORA), OriGene unique variant 1
TC118299 NM_002957 Homo sapiens retinoid X receptor, alpha (RXRA)
TC118079 NM_003250 Homo sapiens thyroid hormone receptor, alpha (erythroblastic leukemia viral (v-erb-a) oncogene
homolog, avian) (THRA), transcript variant 2
TC118080 NM_003250 Homo sapiens thyroid hormone receptor, alpha (erythroblastic leukemia viral (v-erb-a) oncogene
homolog, avian) (THRA), OriGene unique variant 1
TC118089 NM_003269 Homo sapiens nuclear receptor subfamily 2, group E, member 1 (NR2E1)
TC108472 NM_003297 Homo sapiens nuclear receptor subfamily 2, group C, member 1 (NR2C1)
TC118058 NM_003298 Homo sapiens nuclear receptor subfamily 2, group C, member 2 (NR2C2)
TC117736 NM_003822 Homo sapiens nuclear receptor subfamily 5, group A, member 2 (NR5A2), transcript variant 2
TC117737 NM_003822 Homo sapiens nuclear receptor subfamily 5, group A, member 2 (NR5A2), OriGene unique variant 1
TC109996 NM_003889 Homo sapiens nuclear receptor subfamily 1, group I, member 2 (NR1I2), OriGene unique variant 1
TC109997 NM_003889 Homo sapiens nuclear receptor subfamily 1, group I, member 2 (NR1I2), transcript variant 1
TC117545 NM_004133 Homo sapiens hepatocyte nuclear factor 4, gamma (HNF4G)
TC108253 NM_004451 Homo sapiens estrogen-related receptor alpha (ESRRA)
TC123865 NM_004452 Homo sapiens estrogen-related receptor beta (ESRRB)
TC117294 NM_004585 Homo sapiens retinoic acid receptor responder (tazarotene induced) 3 (RARRES3)
TC117168 NM_004774 Homo sapiens PPAR binding protein (PPARBP), OriGene unique variant 1
TC116987 NM_005036 Homo sapiens peroxisome proliferative activated receptor, alpha (PPARA), transcript variant 5
TC111129 NM_005122 Homo sapiens nuclear receptor subfamily 1, group I, member 3 (NR1I3)
TC116941 NM_005123 Homo sapiens nuclear receptor subfamily 1, group H, member 4 (NR1H4)
TC101015 NM_005126 Homo sapiens nuclear receptor subfamily 1, group D, member 2 (NR1D2)
TC116862 NM_005234 Homo sapiens nuclear receptor subfamily 2, group F, member 6 (NR2F6)
Nuclear Hormone Receptor CloneSet
Tru
eClo
ne™
Clon
eSet
:Gen
e-fa
mily
base
dco
llect
ion
32 www.origene.com
Cat. No. Accession No. Description
TC122167 AY054985 Homo sapiens voltage-dependent calcium channel beta-4a subunit mRNA, complete cds
TC122546 NM_000079 Homo sapiens cholinergic receptor, nicotinic, alpha polypeptide 1 (muscle) (CHRNA1)
TC122073 NM_000079 Homo sapiens cholinergic receptor, nicotinic, alpha polypeptide 1 (muscle) (CHRNA1)
TC123958 NM_000080 Homo sapiens cholinergic receptor, nicotinic, epsilon polypeptide (CHRNE)
TC122070 NM_000083 Homo sapiens chloride channel 1, skeletal muscle (Thomsen disease, autosomal dominant) (CLCN1)
TC100001 NM_000084 Homo sapiens chloride channel 5 (nephrolithiasis 2, X-linked, Dent disease) (CLCN5)
TC122040 NM_000087 Homo sapiens cyclic nucleotide gated channel alpha 1 (CNGA1)
TC120084 NM_000165 Homo sapiens gap junction protein, alpha 1, 43kDa (connexin 43) (GJA1)
TC120085 NM_000166 Homo sapiens gap junction protein, beta 1, 32kDa (connexin 32, Charcot-Marie-Tooth neuropathy,
X-linked) (GJB1)
TC123992 NM_000171 Homo sapiens glycine receptor, alpha 1 (startle disease/hyperekplexia, stiff man syndrome) (GLRA1),
OriGene unique variant 1
TC122128 NM_000217 Homo sapiens potassium voltage-gated channel, shaker-related subfamily, member 1 (episodic ataxia
with myokymia) (KCNA1)
TC121930 NM_000219 Homo sapiens potassium voltage-gated channel, Isk-related family, member 1 (KCNE1)
TC122218 NM_000220 Homo sapiens potassium inwardly-rectifying channel, subfamily J, member 1 (KCNJ1), transcript
variant rom-k1
TC123920 NM_000220 Homo sapiens potassium inwardly-rectifying channel, subfamily J, member 1 (KCNJ1), transcript
variant rom-k1
Ion Channel CloneSet
Cat. No. Accession No. Description
TC116616 NM_005654 Homo sapiens nuclear receptor subfamily 2, group F, member 1 (NR2F1)
TC116562 NM_005693 Homo sapiens nuclear receptor subfamily 1, group H, member 3 (NR1H3), OriGene unique variant 1
TC116563 NM_005693 Homo sapiens nuclear receptor subfamily 1, group H, member 3 (NR1H3), OriGene unique variant 2
TC116564 NM_005693 Homo sapiens nuclear receptor subfamily 1, group H, member 3 (NR1H3)
TC116298 NM_006186 Homo sapiens nuclear receptor subfamily 4, group A, member 2 (NR4A2), transcript variant 1
TC111151 NM_006320 Homo sapiens progesterone receptor membrane component 2 (PGRMC2)
TC115976 NM_006601 Homo sapiens unactive progesterone receptor, 23 kD (TEBP)
TC115946 NM_006667 Homo sapiens progesterone receptor membrane component 1 (PGRMC1)
TC115780 NM_006914 Homo sapiens RAR-related orphan receptor B (RORB)
TC115782 NM_006917 Homo sapiens retinoid X receptor, gamma (RXRG), transcript variant 1
TC115804 NM_006981 Homo sapiens nuclear receptor subfamily 4, group A, member 3 (NR4A3), transcript variant 1
TC115734 NM_007121 Homo sapiens nuclear receptor subfamily 1, group H, member 2 (NR1H2), OriGene unique variant 1
TC115735 NM_007121 Homo sapiens nuclear receptor subfamily 1, group H, member 2 (NR1H2)
TC114427 NM_016213 Homo sapiens thyroid hormone receptor interactor 4 (TRIP4), OriGene unique variant 1
TC114428 NM_016213 Homo sapiens thyroid hormone receptor interactor 4 (TRIP4), OriGene unique variant 2
TC114329 NM_016346 Homo sapiens nuclear receptor subfamily 2, group E, member 3 (NR2E3), transcript variant 1
TC108069 NM_021005 Homo sapiens nuclear receptor subfamily 2, group F, member 2 (NR2F2)
TC112578 NM_021724 Homo sapiens nuclear receptor subfamily 1, group D, member 1 (NR1D1)
TC112839 NM_021976 Homo sapiens retinoid X receptor, beta (RXRB)
TC123126 NM_134261 Homo sapiens RAR-related orphan receptor A (RORA), transcript variant 1
TC108192 NM_138711 Homo sapiens peroxisome proliferative activated receptor, gamma (PPARG), transcript variant 3
TC124177 NM_138712 Homo sapiens peroxisome proliferative activated receptor, gamma (PPARG), transcript variant 1
TC109596 NM_177435 Homo sapiens peroxisome proliferative activated receptor, delta (PPARD), transcript variant 2
TC123864 X74497 H.sapiens mRNA for thyroid hormone receptor beta-2 isoform
TC107087 XM_092480 Homo sapiens similar to nuclear receptor subfamily 1, group D, member 1 (LOC151155)
Tru
eClo
ne™
Clon
eSet
:Gen
e-fa
mily
base
dco
llect
ion
Cat. No. Accession No. Description
TC123921 NM_000220 Homo sapiens potassium inwardly-rectifying channel, subfamily J, member 1 (KCNJ1), transcript
variant rom-k1
TC123972 NM_000238 Homo sapiens potassium voltage-gated channel, subfamily H (eag-related), member 2 (KCNH2),
transcript variant 1
TC110976 NM_000297 Homo sapiens polycystic kidney disease 2 (autosomal dominant) (PKD2)
TC124054 NM_000335 Homo sapiens sodium channel, voltage-gated, type V, alpha (long QT syndrome 3) (SCN5A), transcript
variant 2
TC119979 NM_000336 Homo sapiens sodium channel, nonvoltage-gated 1, beta (Liddle syndrome) (SCNN1B)
TC122157 NM_000385 Homo sapiens aquaporin 1 (channel-forming integral protein, 28kDa) (AQP1), transcript variant 2
TC122091 NM_000397 Homo sapiens cytochrome b-245, beta polypeptide (chronic granulomatous disease) (CYBB)
TC123976 NM_000525 Homo sapiens potassium inwardly-rectifying channel, subfamily J, member 11 (KCNJ11)
TC124161 NM_000722 Homo sapiens calcium channel, voltage-dependent, alpha 2/delta subunit 1 (CACNA2D1)
TC123891 NM_000723 Homo sapiens calcium channel, voltage-dependent, beta 1 subunit (CACNB1), transcript variant 1
TC124040 NM_000723 Homo sapiens calcium channel, voltage-dependent, beta 1 subunit (CACNB1), transcript variant 1
TC124059 NM_000724 Homo sapiens calcium channel, voltage-dependent, beta 2 subunit (CACNB2), transcript variant 1
TC122090 NM_000725 Homo sapiens calcium channel, voltage-dependent, beta 3 subunit (CACNB3)
TC119727 NM_000725 Homo sapiens calcium channel, voltage-dependent, beta 3 subunit (CACNB3), OriGene unique variant 1
TC122048 NM_000726 Homo sapiens calcium channel, voltage-dependent, beta 4 subunit (CACNB4), transcript variant 2
TC122189 NM_000727 Homo sapiens calcium channel, voltage-dependent, gamma subunit 1 (CACNG1)
TC123917 NM_000742 Homo sapiens cholinergic receptor, nicotinic, alpha polypeptide 2 (neuronal) (CHRNA2)
TC122160 NM_000743 Homo sapiens cholinergic receptor, nicotinic, alpha polypeptide 3 (CHRNA3)
TC122164 NM_000745 Homo sapiens cholinergic receptor, nicotinic, alpha polypeptide 5 (CHRNA5)
TC108445 NM_000745 Homo sapiens cholinergic receptor, nicotinic, alpha polypeptide 5 (CHRNA5), OriGene unique variant 1
TC124074 NM_000746 Homo sapiens cholinergic receptor, nicotinic, alpha polypeptide 7 (CHRNA7)
TC122081 NM_000746 Homo sapiens cholinergic receptor, nicotinic, alpha polypeptide 7 (CHRNA7)
TC122573 NM_000747 Homo sapiens cholinergic receptor, nicotinic, beta polypeptide 1 (muscle) (CHRNB1)
TC122138 NM_000747 Homo sapiens cholinergic receptor, nicotinic, beta polypeptide 1 (muscle) (CHRNB1), OriGene unique
variant 1
TC119699 NM_000748 Homo sapiens cholinergic receptor, nicotinic, beta polypeptide 2 (neuronal) (CHRNB2)
TC122056 NM_000750 Homo sapiens cholinergic receptor, nicotinic, beta polypeptide 4 (CHRNB4), OriGene unique variant 1
TC122142 NM_000750 Homo sapiens cholinergic receptor, nicotinic, beta polypeptide 4 (CHRNB4), OriGene unique variant 2
TC119700 NM_000751 Homo sapiens cholinergic receptor, nicotinic, delta polypeptide (CHRND)
TC119668 NM_000806 Homo sapiens gamma-aminobutyric acid (GABA) A receptor, alpha 1 (GABRA1)
TC119669 NM_000807 Homo sapiens gamma-aminobutyric acid (GABA) A receptor, alpha 2 (GABRA2)
TC119670 NM_000808 Homo sapiens gamma-aminobutyric acid (GABA) A receptor, alpha 3 (GABRA3)
TC119625 NM_000809 Homo sapiens gamma-aminobutyric acid (GABA) A receptor, alpha 4 (GABRA4)
TC119626 NM_000810 Homo sapiens gamma-aminobutyric acid (GABA) A receptor, alpha 5 (GABRA5)
TC119627 NM_000811 Homo sapiens gamma-aminobutyric acid (GABA) A receptor, alpha 6 (GABRA6)
TC122150 NM_000811 Homo sapiens gamma-aminobutyric acid (GABA) A receptor, alpha 6 (GABRA6), OriGene unique variant 1
TC119628 NM_000812 Homo sapiens gamma-aminobutyric acid (GABA) A receptor, beta 1 (GABRB1)
TC119629 NM_000813 Homo sapiens gamma-aminobutyric acid (GABA) A receptor, beta 2 (GABRB2), transcript variant 2
TC110903 NM_000814 Homo sapiens gamma-aminobutyric acid (GABA) A receptor, beta 3 (GABRB3), transcript variant 1
TC122576 NM_000815 Homo sapiens gamma-aminobutyric acid (GABA) A receptor, delta (GABRD)
TC109261 NM_000816 Homo sapiens gamma-aminobutyric acid (GABA) A receptor, gamma 2 (GABRG2), transcript variant 2
TC119636 NM_000824 Homo sapiens glycine receptor, beta (GLRB)
TC119637 NM_000826 Homo sapiens glutamate receptor, ionotropic, AMPA 2 (GRIA2)
TC119638 NM_000827 Homo sapiens glutamate receptor, ionotropic, AMPA 1 (GRIA1)
TC122080 NM_000828 Homo sapiens glutamate receptor, ionotrophic, AMPA 3 (GRIA3), transcript variant flop
TC119639 NM_000829 Homo sapiens glutamate receptor, ionotrophic, AMPA 4 (GRIA4)
33ph: 1.888.267.4436 fax: 1.301.340.9254
Tru
eClo
ne™
Clon
eSet
:Gen
e-fa
mily
base
dco
llect
ion
34 www.origene.com
Cat. No. Accession No. Description
TC111634 NM_000830 Homo sapiens glutamate receptor, ionotropic, kainate 1 (GRIK1), OriGene unique variant 1
TC122079 NM_000830 Homo sapiens glutamate receptor, ionotropic, kainate 1 (GRIK1), transcript variant 1
TC119640 NM_000831 Homo sapiens glutamate receptor, ionotropic, kainate 3 (GRIK3), OriGene unique variant 1
TC122147 NM_000831 Homo sapiens glutamate receptor, ionotropic, kainate 3 (GRIK3), OriGene unique variant 2
TC119642 NM_000834 Homo sapiens glutamate receptor, ionotropic, N-methyl D-aspartate 2B (GRIN2B)
TC122578 NM_000869 Homo sapiens 5-hydroxytryptamine (serotonin) receptor 3A (HTR3A), transcript variant 2
TC119590 NM_000890 Homo sapiens potassium inwardly-rectifying channel, subfamily J, member 5 (KCNJ5)
TC119591 NM_000891 Homo sapiens potassium inwardly-rectifying channel, subfamily J, member 2 (KCNJ2)
TC121957 NM_001002914 Homo sapiens potassium channel tetramerisation domain containing 11 (KCTD11)
TC119540 NM_001037 Homo sapiens sodium channel, voltage-gated, type I, beta (SCN1B), transcript variant a
TC119542 NM_001038 Homo sapiens sodium channel, nonvoltage-gated 1 alpha (SCNN1A)
TC119543 NM_001039 Homo sapiens sodium channel, nonvoltage-gated 1, gamma (SCNN1G)
TC124011 NM_001094 Homo sapiens amiloride-sensitive cation channel 1, neuronal (degenerin) (ACCN1), transcript variant 2
TC108885 NM_001095 Homo sapiens amiloride-sensitive cation channel 2, neuronal (ACCN2), transcript variant 2
TC123906 NM_001285 Homo sapiens chloride channel, calcium activated, family member 1 (CLCA1)
TC124035 NM_001285 Homo sapiens chloride channel, calcium activated, family member 1 (CLCA1)
TC122134 NM_001286 Homo sapiens chloride channel 6 (CLCN6), transcript variant ClC-6a
TC124176 NM_001286 Homo sapiens chloride channel 6 (CLCN6), transcript variant ClC-6a
TC108053 NM_001287 Homo sapiens chloride channel 7 (CLCN7)
TC122065 NM_001287 Homo sapiens chloride channel 7 (CLCN7), OriGene unique variant 1
TC119324 NM_001288 Homo sapiens chloride intracellular channel 1 (CLIC1)
TC119325 NM_001289 Homo sapiens chloride intracellular channel 2 (CLIC2)
TC122039 NM_001289 Homo sapiens chloride intracellular channel 2 (CLIC2), OriGene unique variant 1
TC119328 NM_001293 Homo sapiens chloride channel, nucleotide-sensitive, 1A (CLNS1A)
TC119289 NM_001298 Homo sapiens cyclic nucleotide gated channel alpha 3 (CNGA3), OriGene unique variant 1
TC123987 NM_001510 Homo sapiens glutamate receptor, ionotropic, delta 2 (GRID2)
TC121946 NM_001680 Homo sapiens FXYD domain containing ion transport regulator 2 (FXYD2), transcript variant a
TC110856 NM_001829 Homo sapiens chloride channel 3 (CLCN3), transcript variant b
TC118976 NM_001830 Homo sapiens chloride channel 4 (CLCN4)
TC123926 NM_002042 Homo sapiens gamma-aminobutyric acid (GABA) receptor, rho 1 (GABRR1), OriGene unique variant 1
TC123929 NM_002043 Homo sapiens gamma-aminobutyric acid (GABA) receptor, rho 2 (GABRR2)
TC118876 NM_002060 Homo sapiens gap junction protein, alpha 4, 37kDa (connexin 37) (GJA4)
TC110908 NM_002063 Homo sapiens glycine receptor, alpha 2 (GLRA2)
TC122177 NM_002063 Homo sapiens glycine receptor, alpha 2 (GLRA2), OriGene unique variant 1
TC120401 NM_002088 Homo sapiens glutamate receptor, ionotropic, kainate 5 (GRIK5)
TC118765 NM_002232 Homo sapiens potassium voltage-gated channel, shaker-related subfamily, member 3 (KCNA3)
TC123916 NM_002233 Homo sapiens potassium voltage-gated channel, shaker-related subfamily, member 4 (KCNA4)
TC123963 NM_002234 Homo sapiens potassium voltage-gated channel, shaker-related subfamily, member 5 (KCNA5),
OriGene unique variant 1
TC123964 NM_002234 Homo sapiens potassium voltage-gated channel, shaker-related subfamily, member 5 (KCNA5)
TC118766 NM_002235 Homo sapiens potassium voltage-gated channel, shaker-related subfamily, member 6 (KCNA6)
TC118767 NM_002236 Homo sapiens potassium voltage-gated channel, subfamily F, member 1 (KCNF1)
TC121929 NM_002237 Homo sapiens potassium voltage-gated channel, subfamily G, member 1 (KCNG1), transcript variant 1
TC118769 NM_002239 Homo sapiens potassium inwardly-rectifying channel, subfamily J, member 3 (KCNJ3)
TC118770 NM_002240 Homo sapiens potassium inwardly-rectifying channel, subfamily J, member 6 (KCNJ6)
TC118741 NM_002241 Homo sapiens potassium inwardly-rectifying channel, subfamily J, member 10 (KCNJ10)
TC101096 NM_002242 Homo sapiens potassium inwardly-rectifying channel, subfamily J, member 13 (KCNJ13), OriGene
unique variant 1
Tru
eClo
ne™
Clon
eSet
:Gen
e-fa
mily
base
dco
llect
ion
35ph: 1.888.267.4436 fax: 1.301.340.9254
Cat. No. Accession No. Description
TC122174 NM_002242 Homo sapiens potassium inwardly-rectifying channel, subfamily J, member 13 (KCNJ13)
TC122057 NM_002242 Homo sapiens potassium inwardly-rectifying channel, subfamily J, member 13 (KCNJ13), OriGene
unique variant 2
TC118712 NM_002243 Homo sapiens potassium inwardly-rectifying channel, subfamily J, member 15 (KCNJ15), transcript
variant 2
TC110937 NM_002245 Homo sapiens potassium channel, subfamily K, member 1 (KCNK1)
TC118713 NM_002246 Homo sapiens potassium channel, subfamily K, member 3 (KCNK3)
TC122141 NM_002247 Homo sapiens potassium large conductance calcium-activated channel, subfamily M, alpha member 1
(KCNMA1), OriGene unique variant 3
TC122094 NM_002247 Homo sapiens potassium large conductance calcium-activated channel, subfamily M, alpha member 1
(KCNMA1), OriGene unique variant 2
TC122078 NM_002247 Homo sapiens potassium large conductance calcium-activated channel, subfamily M, alpha member 1
(KCNMA1), OriGene unique variant 1
TC124003 NM_002248 Homo sapiens potassium intermediate/small conductance calcium-activated channel, subfamily N,
member 1 (KCNN1)
TC122055 NM_002250 Homo sapiens potassium intermediate/small conductance calcium-activated channel, subfamily N,
member 4 (KCNN4), OriGene unique variant 1
TC121952 NM_002250 Homo sapiens potassium intermediate/small conductance calcium-activated channel, subfamily N,
member 4 (KCNN4)
TC118715 NM_002252 Homo sapiens potassium voltage-gated channel, delayed-rectifier, subfamily S, member 3 (KCNS3)
TC118594 NM_002558 Homo sapiens purinergic receptor P2X, ligand-gated ion channel, 1 (P2RX1)
TC123980 NM_002559 Homo sapiens purinergic receptor P2X, ligand-gated ion channel, 3 (P2RX3)
TC122124 NM_002560 Homo sapiens purinergic receptor P2X, ligand-gated ion channel, 4 (P2RX4), transcript variant 1
TC121945 NM_002560 Homo sapiens purinergic receptor P2X, ligand-gated ion channel, 4 (P2RX4), OriGene unique
variant 1
TC109527 NM_002561 Homo sapiens purinergic receptor P2X, ligand-gated ion channel, 5 (P2RX5), transcript variant 1
TC118597 NM_002562 Homo sapiens purinergic receptor P2X, ligand-gated ion channel, 7 (P2RX7), transcript variant 1
TC122064 NM_002562 Homo sapiens purinergic receptor P2X, ligand-gated ion channel, 7 (P2RX7), OriGene unique variant 1
TC118315 NM_002978 Homo sapiens sodium channel, nonvoltage-gated 1, delta (SCNN1D), OriGene unique variant 1
TC122116 NM_002978 Homo sapiens sodium channel, nonvoltage-gated 1, delta (SCNN1D), OriGene unique variant 2
TC122139 NM_002978 Homo sapiens sodium channel, nonvoltage-gated 1, delta (SCNN1D)
TC121934 NM_003304 Homo sapiens transient receptor potential cation channel, subfamily C, member 1 (TRPC1)
TC124002 NM_003305 Homo sapiens transient receptor potential cation channel, subfamily C, member 3 (TRPC3), OriGene
unique variant 1
TC123988 NM_003374 Homo sapiens voltage-dependent anion channel 1 (VDAC1)
TC117987 NM_003374 Homo sapiens voltage-dependent anion channel 1 (VDAC1)
TC117988 NM_003375 Homo sapiens voltage-dependent anion channel 2 (VDAC2)
TC123984 NM_003471 Homo sapiens potassium voltage-gated channel, shaker-related subfamily, beta member 1 (KCNAB1),
transcript variant 2
TC124159 NM_003740 Homo sapiens potassium channel, subfamily K, member 5 (KCNK5)
TC122666 NM_004004 Homo sapiens gap junction protein, beta 2, 26kDa (connexin 26) (GJB2)
TC117547 NM_004137 Homo sapiens potassium large conductance calcium-activated channel, subfamily M, beta member 1
(KCNMB1)
TC122132 NM_004183 Homo sapiens vitelliform macular dystrophy (Best disease, bestrophin) (VMD2), OriGene unique variant 1
TC124153 NM_004198 Homo sapiens cholinergic receptor, nicotinic, alpha polypeptide 6 (CHRNA6)
TC117417 NM_004366 Homo sapiens chloride channel 2 (CLCN2)
TC122684 NM_004588 Homo sapiens sodium channel, voltage-gated, type II, beta (SCN2B)
TC122148 NM_004621 Homo sapiens transient receptor potential cation channel, subfamily C, member 6 (TRPC6), OriGene
unique variant 1
TC111045 NM_004621 Homo sapiens transient receptor potential cation channel, subfamily C, member 6 (TRPC6)
TC122133 NM_004669 Homo sapiens chloride intracellular channel 3 (CLIC3)
Tru
eClo
ne™
Clon
eSet
:Gen
e-fa
mily
base
dco
llect
ion
36 www.origene.com
Cat. No. Accession No. Description
TC122181 NM_004732 Homo sapiens potassium voltage-gated channel, shaker-related subfamily, beta member 3 (KCNAB3),
OriGene unique variant 1
TC111730 NM_004769 Homo sapiens amiloride-sensitive cation channel 3 (ACCN3), transcript variant 1
TC117126 NM_004823 Homo sapiens potassium channel, subfamily K, member 6 (KCNK6)
TC124000 NM_004961 Homo sapiens gamma-aminobutyric acid (GABA) A receptor, epsilon (GABRE), OriGene unique
variant 1
TC117021 NM_004975 Homo sapiens potassium voltage-gated channel, Shab-related subfamily, member 1 (KCNB1)
TC124010 NM_004976 Homo sapiens potassium voltage-gated channel, Shaw-related subfamily, member 1 (KCNC1)
TC111648 NM_004978 Homo sapiens potassium voltage-gated channel, Shaw-related subfamily, member 4 (KCNC4),
transcript variant 1
TC122175 NM_004980 Homo sapiens potassium voltage-gated channel, Shal-related subfamily, member 3 (KCND3), OriGene
unique variant 1
TC123960 NM_004981 Homo sapiens potassium inwardly-rectifying channel, subfamily J, member 4 (KCNJ4), transcript
variant 2
TC117022 NM_004982 Homo sapiens potassium inwardly-rectifying channel, subfamily J, member 8 (KCNJ8)
TC117023 NM_004983 Homo sapiens potassium inwardly-rectifying channel, subfamily J, member 9 (KCNJ9)
TC121958 NM_005031 Homo sapiens FXYD domain containing ion transport regulator 1 (phospholemman) (FXYD1),
transcript variant a
TC122072 NM_005199 Homo sapiens cholinergic receptor, nicotinic, gamma polypeptide (CHRNG)
TC123931 NM_005267 Homo sapiens gap junction protein, alpha 8, 50kDa (connexin 50) (GJA8), OriGene unique variant 1
TC124154 NM_005268 Homo sapiens gap junction protein, beta 5 (connexin 31.1) (GJB5)
TC123978 NM_005446 Homo sapiens purinergic receptor P2X-like 1, orphan receptor (P2RXL1), OriGene unique variant 1
TC111131 NM_005472 Homo sapiens potassium voltage-gated channel, Isk-related family, member 3 (KCNE3)
TC122088 NM_005497 Homo sapiens gap junction protein, alpha 7, 45kDa (connexin 45) (GJA7)
TC124007 NM_005549 Homo sapiens potassium voltage-gated channel, shaker-related subfamily, member 10 (KCNA10)
TC116623 NM_005662 Homo sapiens voltage-dependent anion channel 3 (VDAC3), OriGene unique variant 1
TC122051 NM_005662 Homo sapiens voltage-dependent anion channel 3 (VDAC3)
TC121947 NM_005845 Homo sapiens ATP-binding cassette, sub-family C (CFTR/MRP), member 4 (ABCC4)
TC124178 NM_005845 Homo sapiens ATP-binding cassette, sub-family C (CFTR/MRP), member 4 (ABCC4)
TC116402 NM_005971 Homo sapiens FXYD domain containing ion transport regulator 3 (FXYD3), transcript variant 1
TC124025 NM_006030 Homo sapiens calcium channel, voltage-dependent, alpha 2/delta subunit 2 (CACNA2D2), transcript
variant 2
TC123953 NM_006078 Homo sapiens calcium channel, voltage-dependent, gamma subunit 2 (CACNG2)
TC116336 NM_006114 Homo sapiens translocase of outer mitochondrial membrane 40 homolog (yeast) (TOMM40)
TC116023 NM_006536 Homo sapiens chloride channel, calcium activated, family member 2 (CLCA2)
TC116025 NM_006539 Homo sapiens calcium channel, voltage-dependent, gamma subunit 3 (CACNG3)
TC123944 NM_007052 Homo sapiens NADPH oxidase 1 (NOX1), transcript variant NOH-1L
TC123959 NM_007052 Homo sapiens NADPH oxidase 1 (NOX1), transcript variant NOH-1L
TC115601 NM_007327 Homo sapiens glutamate receptor, ionotropic, N-methyl D-aspartate 1 (GRIN1), transcript variant NR1-3
TC124028 NM_007332 Homo sapiens transient receptor potential cation channel, subfamily A, member 1 (TRPA1)
TC123966 NM_012128 Homo sapiens chloride channel, calcium activated, family member 4 (CLCA4)
TC122153 NM_012226 Homo sapiens purinergic receptor P2X, ligand-gated ion channel, 2 (P2RX2), OriGene unique variant 1
TC115436 NM_012281 Homo sapiens potassium voltage-gated channel, Shal-related subfamily, member 2 (KCND2)
TC122784 NM_012282 Homo sapiens KCNE1-like (KCNE1L)
TC122179 NM_012283 Homo sapiens potassium voltage-gated channel, subfamily G, member 2 (KCNG2), OriGene unique
variant 1
TC122037 NM_012283 Homo sapiens potassium voltage-gated channel, subfamily G, member 2 (KCNG2)
TC122182 NM_012285 Homo sapiens potassium voltage-gated channel, subfamily H (eag-related), member 4 (KCNH4)
TC111208 NM_012329 Homo sapiens monocyte to macrophage differentiation-associated (MMD)
TC110362 NM_012417 Homo sapiens phosphatidylinositol transfer protein, cytoplasmic 1 (PITPNC1), transcript variant 1
Tru
eClo
ne™
Clon
eSet
:Gen
e-fa
mily
base
dco
llect
ion
37ph: 1.888.267.4436 fax: 1.301.340.9254
Cat. No. Accession No. Description
TC115226 NM_013943 Homo sapiens chloride intracellular channel 4 (CLIC4)
TC115114 NM_014164 Homo sapiens FXYD domain containing ion transport regulator 5 (FXYD5), transcript variant 2
TC115134 NM_014211 Homo sapiens gamma-aminobutyric acid (GABA) A receptor, pi (GABRP)
TC122082 NM_014217 Homo sapiens potassium channel, subfamily K, member 2 (KCNK2)
TC115023 NM_014379 Homo sapiens potassium channel, subfamily V, member 1 (KCNV1)
TC122095 NM_014405 Homo sapiens calcium channel, voltage-dependent, gamma subunit 4 (CACNG4)
TC123934 NM_014407 Homo sapiens potassium large conductance calcium-activated channel, subfamily M beta member 3
(KCNMB3), OriGene unique variant 1
TC123923 NM_014407 Homo sapiens potassium large conductance calcium-activated channel, subfamily M beta member 3
(KCNMB3), transcript variant 4
TC108188 NM_014505 Homo sapiens potassium large conductance calcium-activated channel, subfamily M, beta member 4
(KCNMB4)
TC114946 NM_014592 Homo sapiens Kv channel interacting protein 1 (KCNIP1)
TC111199 NM_015127 Homo sapiens Mid-1-related chloride channel 1 (MCLC), OriGene unique variant 1
TC122186 NM_015127 Homo sapiens Mid-1-related chloride channel 1 (MCLC), OriGene unique variant 2
TC122089 NM_015127 Homo sapiens Mid-1-related chloride channel 1 (MCLC)
TC121941 NM_015532 Homo sapiens glutamate receptor, ionotropic, N-methyl D-aspartate-like 1A (GRINL1A)
TC122109 NM_015532 Homo sapiens glutamate receptor, ionotropic, N-methyl D-aspartate-like 1A (GRINL1A), OriGene
unique variant 1
TC111264 NM_015993 Homo sapiens transmembrane 4 superfamily member 11 (plasmolipin) (TM4SF11)
TC122840 NM_016112 Homo sapiens polycystic kidney disease 2-like 1 (PKD2L1)
TC122207 NM_016112 Homo sapiens polycystic kidney disease 2-like 1 (PKD2L1)
TC114461 NM_016113 Homo sapiens transient receptor potential cation channel, subfamily V, member 2 (TRPV2)
TC114411 NM_016179 Homo sapiens transient receptor potential cation channel, subfamily C, member 4 (TRPC4)
TC122193 NM_016318 Homo sapiens purinergic receptor P2X, ligand-gated ion channel, 2 (P2RX2), transcript variant 3
TC114228 NM_016582 Homo sapiens solute carrier family 15, member 3 (SLC15A3)
TC122061 NM_016611 Homo sapiens potassium channel, subfamily K, member 4 (KCNK4), transcript variant 1
TC114142 NM_016929 Homo sapiens chloride intracellular channel 5 (CLIC5)
TC122137 NM_017419 Homo sapiens amiloride-sensitive cation channel 5, intestinal (ACCN5)
TC122085 NM_017581 Homo sapiens cholinergic receptor, nicotinic, alpha polypeptide 9 (CHRNA9)
TC123991 NM_017636 Homo sapiens transient receptor potential cation channel, subfamily M, member 4 (TRPM4)
TC122209 NM_017901 Homo sapiens two pore segment channel 1 (TPCN1)
TC122156 NM_017901 Homo sapiens two pore segment channel 1 (TPCN1), OriGene unique variant 1
TC124160 NM_018298 Homo sapiens mucolipin 3 (MCOLN3)
TC113489 NM_018398 Homo sapiens calcium channel, voltage-dependent, alpha 2/delta 3 subunit (CACNA2D3), OriGene
unique variant 1
TC121942 NM_018398 Homo sapiens calcium channel, voltage-dependent, alpha 2/delta 3 subunit (CACNA2D3)
TC113490 NM_018400 Homo sapiens sodium channel, voltage-gated, type III, beta (SCN3B)
TC122106 NM_018400 Homo sapiens sodium channel, voltage-gated, type III, beta (SCN3B), OriGene unique variant 1
TC120633 NM_018646 Homo sapiens transient receptor potential cation channel, subfamily V, member 6 (TRPV6)
TC123999 NM_018658 Homo sapiens potassium inwardly-rectifying channel, subfamily J, member 16 (KCNJ16), OriGene
unique variant 1
TC122197 NM_018674 Homo sapiens amiloride-sensitive cation channel 4, pituitary (ACCN4), transcript variant 1
TC122126 NM_018727 Homo sapiens transient receptor potential cation channel, subfamily V, member 1 (TRPV1), transcript
variant 2
TC123895 NM_018727 Homo sapiens transient receptor potential cation channel, subfamily V, member 1 (TRPV1), transcript
variant 2
TC123887 NM_018727 Homo sapiens transient receptor potential cation channel, subfamily V, member 1 (TRPV1), transcript
variant 2
TC113285 NM_018992 Homo sapiens potassium channel tetramerisation domain containing 5 (KCTD5), OriGene unique
variant 1
Tru
eClo
ne™
Clon
eSet
:Gen
e-fa
mily
base
dco
llect
ion
38 www.origene.com
Cat. No. Accession No. Description
TC122108 NM_019841 Homo sapiens transient receptor potential cation channel, subfamily V, member 5 (TRPV5)
TC122099 NM_019842 Homo sapiens potassium voltage-gated channel, KQT-like subfamily, member 5 (KCNQ5)
TC122113 NM_020039 Homo sapiens amiloride-sensitive cation channel 2, neuronal (ACCN2), transcript variant 1
TC113118 NM_020389 Homo sapiens transient receptor potential cation channel, subfamily C, member 7 (TRPC7)
TC123975 NM_020402 Homo sapiens cholinergic receptor, nicotinic, alpha polypeptide 10 (CHRNA10)
TC122118 NM_020435 Homo sapiens gap junction protein, alpha 12, 47kDa (GJA12)
TC122214 NM_020659 Homo sapiens tweety homolog 1 (Drosophila) (TTYH1), OriGene unique variant 1
TC122104 NM_020660 Homo sapiens connexin-36 (CX36)
TC121938 NM_020897 Homo sapiens hyperpolarization activated cyclic nucleotide-gated potassium channel 3 (HCN3)
TC121953 NM_020952 Homo sapiens transient receptor potential cation channel, subfamily M, member 3 (TRPM3),
transcript variant 1
TC124157 NM_021012 Homo sapiens potassium inwardly-rectifying channel, subfamily J, member 12 (KCNJ12)
TC122166 NM_021072 Homo sapiens hyperpolarization activated cyclic nucleotide-gated potassium channel 1 (HCN1)
TC112982 NM_021137 Homo sapiens tumor necrosis factor, alpha-induced protein 1 (endothelial) (TNFAIP1)
TC110477 NM_021161 Homo sapiens potassium channel, subfamily K, member 10 (KCNK10), transcript variant 1
TC112899 NM_021614 Homo sapiens potassium intermediate/small conductance calcium-activated channel, subfamily N,
member 2 (KCNN2), OriGene unique variant 1
TC109579 NM_021902 Homo sapiens FXYD domain containing ion transport regulator 1 (phospholemman) (FXYD1), transcript
variant b
TC109260 NM_021912 Homo sapiens gamma-aminobutyric acid (GABA) A receptor, beta 3 (GABRB3), transcript variant 2
TC122077 NM_021912 Homo sapiens gamma-aminobutyric acid (GABA) A receptor, beta 3 (GABRB3), OriGene unique
variant 1
TC123942 NM_021954 Homo sapiens gap junction protein, alpha 3, 46kDa (connexin 46) (GJA3), OriGene unique variant 1
TC121951 NM_021956 Homo sapiens glutamate receptor, ionotropic, kainate 2 (GRIK2), transcript variant 1
TC123967 NM_021984 Homo sapiens gamma-aminobutyric acid (GABA) A receptor, epsilon (GABRE), transcript variant 2
TC122169 NM_022003 Homo sapiens FXYD domain containing ion transport regulator 6 (FXYD6), OriGene unique variant 1
TC112567 NM_022003 Homo sapiens FXYD domain containing ion transport regulator 6 (FXYD6)
TC112568 NM_022006 Homo sapiens FXYD domain containing ion transport regulator 7 (FXYD7), OriGene unique variant 1
TC112856 NM_022054 Homo sapiens potassium channel, subfamily K, member 13 (KCNK13), OriGene unique variant 1
TC122152 NM_022055 Homo sapiens potassium channel, subfamily K, member 12 (KCNK12)
TC122205 NM_022358 Homo sapiens potassium channel, subfamily K, member 15 (KCNK15), OriGene unique variant 1
TC112346 NM_023930 Homo sapiens potassium channel tetramerisation domain containing 14 (KCTD14)
TC112319 NM_024009 Homo sapiens gap junction protein, beta 3, 31kDa (connexin 31) (GJB3), transcript variant 1
TC112300 NM_024047 Homo sapiens nudix (nucleoside diphosphate linked moiety X)-type motif 9 (NUDT9), transcript variant 1
TC112315 NM_024076 Homo sapiens potassium channel tetramerisation domain containing 15 (KCTD15), OriGene unique
variant 1
TC122168 NM_024076 Homo sapiens potassium channel tetramerisation domain containing 15 (KCTD15)
TC122185 NM_024080 Homo sapiens transient receptor potential cation channel, subfamily M, member 8 (TRPM8), OriGene
unique variant 1
TC112270 NM_024080 Homo sapiens transient receptor potential cation channel, subfamily M, member 8 (TRPM8)
TC123954 NM_024505 Homo sapiens NADPH oxidase, EF hand calcium-binding domain 5 (NOX5)
TC122966 NM_024681 Homo sapiens hypothetical protein FLJ12242 (FLJ12242)
TC111844 NM_025221 Homo sapiens Kv channel interacting protein 4 (KCNIP4), transcript variant 1
TC123918 NM_030779 Homo sapiens potassium voltage-gated channel, subfamily H (eag-related), member 6 (KCNH6),
transcript variant 1
TC122060 NM_030779 Homo sapiens potassium voltage-gated channel, subfamily H (eag-related), member 6 (KCNH6),
OriGene unique variant 1
TC123004 NM_031460 Homo sapiens potassium channel, subfamily K, member 17 (KCNK17)
TC122154 NM_031460 Homo sapiens potassium channel, subfamily K, member 17 (KCNK17)
TC122190 NM_031896 Homo sapiens calcium channel, voltage-dependent, gamma subunit 7 (CACNG7)
Tru
eClo
ne™
Clon
eSet
:Gen
e-fa
mily
base
dco
llect
ion
39ph: 1.888.267.4436 fax: 1.301.340.9254
Cat. No. Accession No. Description
TC122107 NM_031896 Homo sapiens calcium channel, voltage-dependent, gamma subunit 7 (CACNG7), OriGene unique
variant 1
TC122144 NM_031954 Homo sapiens potassium channel tetramerisation domain containing 10 (KCTD10), OriGene unique
variant 1
TC106747 NM_031954 Homo sapiens potassium channel tetramerisation domain containing 10 (KCTD10)
TC122202 NM_031954 Homo sapiens potassium channel tetramerisation domain containing 10 (KCTD10), OriGene unique
variant 2
TC106147 NM_032174 Homo sapiens hypothetical protein FLJ12770 (FLJ12770)
TC101325 NM_033223 Homo sapiens gamma-aminobutyric acid (GABA) A receptor, gamma 3 (GABRG3), OriGene unique
variant 1
TC122076 NM_033223 Homo sapiens gamma-aminobutyric acid (GABA) A receptor, gamma 3 (GABRG3)
TC122210 NM_033347 Homo sapiens potassium channel, subfamily K, member 7 (KCNK7), transcript variant A
TC111509 NM_054020 Homo sapiens cation channel, sperm associated 2 (CATSPER2), transcript variant 1
TC123112 NM_080671 Homo sapiens potassium voltage-gated channel, Isk-related family, member 4 (KCNE4)
TC122110 NM_130770 Homo sapiens 5-hydroxytryptamine (serotonin) receptor 3, family member C (HTR3C), OriGene unique
variant 2
TC122044 NM_130770 Homo sapiens 5-hydroxytryptamine (serotonin) receptor 3, family member C (HTR3C), OriGene unique
variant 1
TC122066 NM_133445 Homo sapiens glutamate receptor, ionotropic, N-methyl-D-aspartate 3A (GRIN3A)
TC123125 NM_133490 Homo sapiens potassium voltage-gated channel, subfamily G, member 4 (KCNG4), transcript variant 2
TC123935 NM_133497 Homo sapiens potassium channel, subfamily V, member 2 (KCNV2)
TC121923 NM_138317 Homo sapiens potassium channel, subfamily K, member 10 (KCNK10), transcript variant 2
TC122074 NM_138318 Homo sapiens potassium channel, subfamily K, member 10 (KCNK10), transcript variant 3
TC122216 NM_138392 Homo sapiens SH3KBP1 binding protein 1 (SHKBP1)
TC120461 NM_138444 Homo sapiens potassium channel tetramerisation domain containing 12 (KCTD12)
TC122067 NM_139075 Homo sapiens two pore segment channel 2 (TPCN2), OriGene unique variant 1
TC122042 NM_139075 Homo sapiens two pore segment channel 2 (TPCN2)
TC122136 NM_139075 Homo sapiens two pore segment channel 2 (TPCN2), OriGene unique variant 2
TC109363 NM_139137 Homo sapiens potassium voltage-gated channel, Shaw-related subfamily, member 2 (KCNC2), transcript
variant 2
TC120679 NM_139202 Homo sapiens megalencephalic leukoencephalopathy with subcortical cysts 1 (MLC1), transcript variant 2
TC122059 NM_139318 Homo sapiens potassium voltage-gated channel, subfamily H (eag-related), member 5 (KCNH5),
OriGene unique variant 1
TC122054 NM_139320 Homo sapiens CHRNA7 (cholinergic receptor, nicotinic, alpha polypeptide 7, exons 5-10) and FAM7A
(family with sequence similarity 7A, exons A-E) fusion (CHRFAM7A), transcript variant 1
TC124168 NM_139320 Homo sapiens CHRNA7 (cholinergic receptor, nicotinic, alpha polypeptide 7, exons 5-10) and FAM7A
(family with sequence similarity 7A, exons A-E) fusion (CHRFAM7A), transcript variant 1
TC100185 NM_144633 Homo sapiens potassium voltage-gated channel, subfamily H (eag-related), member 8 (KCNH8)
TC122083 NM_144779 Homo sapiens FXYD domain containing ion transport regulator 5 (FXYD5), transcript variant 1
TC122191 NM_145068 Homo sapiens transient receptor potential cation channel, subfamily V, member 3 (TRPV3)
TC123234 NM_145814 Homo sapiens calcium channel, voltage-dependent, gamma subunit 6 (CACNG6), transcript variant 1
TC124006 NM_145814 Homo sapiens calcium channel, voltage-dependent, gamma subunit 6 (CACNG6), transcript variant 1
TC122178 NM_145815 Homo sapiens calcium channel, voltage-dependent, gamma subunit 6 (CACNG6), transcript variant 2
TC122145 NM_147181 Homo sapiens Kv channel interacting protein 4 (KCNIP4), OriGene unique variant 1
TC110645 NM_147183 Homo sapiens Kv channel interacting protein 4 (KCNIP4), transcript variant 4
TC123940 NM_147204 Homo sapiens transient receptor potential cation channel, subfamily V, member 4 (TRPV4), transcript
variant 2
TC100579 NM_152387 Homo sapiens hypothetical protein FLJ31322 (FLJ31322)
TC122063 NM_152387 Homo sapiens hypothetical protein FLJ31322 (FLJ31322), OriGene unique variant 1
TC100666 NM_152439 Homo sapiens vitelliform macular dystrophy 2-like 3 (VMD2L3)
Tru
eClo
ne™
Clon
eSet
:Gen
e-fa
mily
base
dco
llect
ion
Cat. No. Accession No. Description
TC123919 NM_152868 Homo sapiens potassium inwardly-rectifying channel, subfamily J, member 4 (KCNJ4), transcript
variant 1
TC100993 NM_153033 Homo sapiens potassium channel tetramerisation domain containing 7 (KCTD7)
TC123292 NM_153212 Homo sapiens gap junction protein, beta 4 (connexin 30.3) (GJB4)
TC122098 NM_153212 Homo sapiens gap junction protein, beta 4 (connexin 30.3) (GJB4)
TC122213 NM_153274 Homo sapiens vitelliform macular dystrophy 2-like 2 (VMD2L2), OriGene unique variant 1
TC100338 NM_153331 Homo sapiens potassium channel tetramerisation domain containing 6 (KCTD6)
TC109364 NM_153748 Homo sapiens potassium voltage-gated channel, Shaw-related subfamily, member 2 (KCNC2),
transcript variant 3
TC124158 NM_153765 Homo sapiens potassium inwardly-rectifying channel, subfamily J, member 1 (KCNJ1), transcript
variant rom-k3
TC122199 NM_153767 Homo sapiens potassium inwardly-rectifying channel, subfamily J, member 1 (KCNJ1), transcript
variant rom-k5
TC123939 NM_153767 Homo sapiens potassium inwardly-rectifying channel, subfamily J, member 1 (KCNJ1), transcript
variant rom-k5
TC122195 NM_170683 Homo sapiens purinergic receptor P2X, ligand-gated ion channel, 2 (P2RX2), OriGene unique variant 1
TC122206 NM_170720 Homo sapiens potassium inwardly-rectifying channel, subfamily J, member 14 (KCNJ14), transcript
variant 2
TC122146 NM_170742 Homo sapiens potassium inwardly-rectifying channel, subfamily J, member 16 (KCNJ16), transcript
variant 3
TC124009 NM_170782 Homo sapiens potassium intermediate/small conductance calcium-activated channel, subfamily N,
member 3 (KCNN3), OriGene unique variant 1
TC123922 NM_171828 Homo sapiens potassium large conductance calcium-activated channel, subfamily M beta member 3
(KCNMB3), transcript variant 1
TC123933 NM_171829 Homo sapiens potassium large conductance calcium-activated channel, subfamily M beta member 3
(KCNMB3), transcript variant 2
TC123979 NM_172056 Homo sapiens potassium voltage-gated channel, subfamily H (eag-related), member 2 (KCNH2),
transcript variant 2
TC123313 NM_172056 Homo sapiens potassium voltage-gated channel, subfamily H (eag-related), member 2 (KCNH2),
transcript variant 2
TC124103 NM_172057 Homo sapiens potassium voltage-gated channel, subfamily H (eag-related), member 2 (KCNH2),
transcript variant 3
TC109366 NM_172057 Homo sapiens potassium voltage-gated channel, subfamily H (eag-related), member 2 (KCNH2),
OriGene unique variant 1
TC122127 NM_172097 Homo sapiens cation channel, sperm associated 2 (CATSPER2), OriGene unique variant 1
TC122172 NM_172097 Homo sapiens cation channel, sperm associated 2 (CATSPER2), OriGene unique variant 2
TC122047 NM_172109 Homo sapiens potassium voltage-gated channel, KQT-like subfamily, member 2 (KCNQ2), transcript
variant 5
TC123941 NM_172130 Homo sapiens potassium voltage-gated channel, shaker-related subfamily, beta member 2 (KCNAB2),
transcript variant 2
TC122111 NM_172159 Homo sapiens potassium voltage-gated channel, shaker-related subfamily, beta member 1 (KCNAB1),
transcript variant 3
TC109907 NM_172159 Homo sapiens potassium voltage-gated channel, shaker-related subfamily, beta member 1 (KCNAB1),
transcript variant 3
TC123965 NM_172364 Homo sapiens calcium channel, voltage-dependent, alpha 2/delta subunit 4 (CACNA2D4), transcript
variant 1
TC111240 NM_172376 Homo sapiens potassium voltage-gated channel, subfamily H (eag-related), member 5 (KCNH5),
transcript variant 2
TC122092 NM_172376 Homo sapiens potassium voltage-gated channel, subfamily H (eag-related), member 5 (KCNH5),
transcript variant 2
TC101212 NM_173160 Homo sapiens FXYD domain containing ion transport regulator 4 (FXYD4), OriGene unique variant 1
TC122143 NM_173162 Homo sapiens potassium voltage-gated channel, subfamily H (eag-related), member 7 (KCNH7),
transcript variant 2
TC122112 NM_173195 Homo sapiens Kv channel interacting protein 2 (KCNIP2), transcript variant 6
40 www.origene.com
Tru
eClo
ne™
Clon
eSet
:Gen
e-fa
mily
base
dco
llect
ion
Cat. No. Accession No. Description
TC101135 NM_173536 Homo sapiens gamma-aminobutyric acid (GABA) A receptor, gamma 1 (GABRG1)
TC124180 NM_173562 Homo sapiens chromosome 6 open reading frame 69 (C6orf69)
TC122121 NM_173562 Homo sapiens chromosome 6 open reading frame 69 (C6orf69), OriGene unique variant 2
TC122096 NM_173562 Homo sapiens chromosome 6 open reading frame 69 (C6orf69), OriGene unique variant 1
TC122103 NM_173872 Homo sapiens chloride channel 3 (CLCN3), OriGene unique variant 1
TC122102 NM_174873 Homo sapiens purinergic receptor P2X, ligand-gated ion channel, 2 (P2RX2), transcript variant 2
TC123982 NM_174934 Homo sapiens sodium channel, voltage-gated, type IV, beta (SCN4B)
TC108426 NM_175568 Homo sapiens purinergic receptor P2X, ligand-gated ion channel, 4 (P2RX4), transcript variant 3
TC121962 NM_175611 Homo sapiens glutamate receptor, ionotropic, kainate 1 (GRIK1), transcript variant 2
TC109282 NM_175768 Homo sapiens glutamate receptor, ionotropic, kainate 2 (GRIK2), OriGene unique variant 1
TC122097 NM_177427 Homo sapiens purinergic receptor P2X, ligand-gated ion channel, 7 (P2RX7), OriGene unique variant 1
TC123945 NM_178019 Homo sapiens cation channel, sperm associated 3 (CATSPER3)
TC107237 NM_178863 Homo sapiens potassium channel tetramerisation domain containing 13 (KCTD13)
TC122050 NM_180990 Homo sapiens ligand-gated ion channel subunit (LGICZ)
TC122192 NM_181671 Homo sapiens phosphatidylinositol transfer protein, cytoplasmic 1 (PITPNC1), transcript variant 2
TC122130 NM_181894 Homo sapiens glutamate receptor, ionotrophic, AMPA 3 (GRIA3), OriGene unique variant 1
TC107729 NM_198039 Homo sapiens nudix (nucleoside diphosphate linked moiety X)-type motif 9 (NUDT9), transcript variant 2
TC121943 NM_198098 Homo sapiens aquaporin 1 (channel-forming integral protein, 28kDa) (AQP1), transcript variant 1
TC121140 NM_198991 Homo sapiens potassium channel tetramerisation domain containing 1 (KCTD1)
TC122075 NM_198991 Homo sapiens potassium channel tetramerisation domain containing 1 (KCTD1), OriGene unique variant 1
TC121950 NM_199248 Homo sapiens calcium channel, voltage-dependent, beta 1 subunit (CACNB1), transcript variant 3
TC122187 NM_201570 Homo sapiens calcium channel, voltage-dependent, beta 2 subunit (CACNB2), transcript variant 7
TC122200 NM_201571 Homo sapiens calcium channel, voltage-dependent, beta 2 subunit (CACNB2), transcript variant 6
TC122135 NM_201572 Homo sapiens calcium channel, voltage-dependent, beta 2 subunit (CACNB2), OriGene unique variant 1
TC123990 XM_029962 PREDICTED: Homo sapiens potassium channel, subfamily T, member 1 (KCNT1)
TC121935 XM_043613 PREDICTED: Homo sapiens glutamate receptor, ionotropic, delta 1 (GRID1)
TC122041 XM_086565 Homo sapiens hyperpolarization activated cyclic nucleotide-gated potassium channel 3 (HCN3)
TC122071 XM_086565 Homo sapiens hyperpolarization activated cyclic nucleotide-gated potassium channel 3 (HCN3), OriGene
unique variant 1
TC121931 XM_291095 PREDICTED: Homo sapiens similar to glutamate receptor, ionotropic, N-methyl D-aspartate-like 1A
(LOC339970)
TC123930 XM_371263 PREDICTED: Homo sapiens mucolipin 2 (MCOLN2)
TC122173 XR_000147 PREDICTED: Homo sapiens transient receptor potential cation channel, subfamily C, member 2
(TRPC2), misc RNA
P450 Clone SetCat. No. Accession No. Description
TC102224 NM_000102 Homo sapiens cytochrome P450, family 17, subfamily A, polypeptide 1 (CYP17A1)
TC103335 NM_000104 Homo sapiens cytochrome P450, family 1, subfamily B, polypeptide 1 (CYP1B1)
TC104446 NM_000106 Homo sapiens cytochrome P450, family 2, subfamily D, polypeptide 6 (CYP2D6)
TC120793 NM_000106 Homo sapiens cytochrome P450, family 2, subfamily D, polypeptide 6 (CYP2D6), OriGene unique
variant 1
TC123879 NM_000497 Homo sapiens cytochrome P450, family 11, subfamily B, polypeptide 1 (CYP11B1), nuclear gene
encoding mitochondrial protein
TC119858 NM_000499 Homo sapiens cytochrome P450, family 1, subfamily A, polypeptide 1 (CYP1A1)
41ph: 1.888.267.4436 fax: 1.301.340.9254
Tru
eClo
ne™
Clon
eSet
:Gen
e-fa
mily
base
dco
llect
ion
42 www.origene.com
Cat. No. Accession No. Description
TC119859 NM_000500 Homo sapiens cytochrome P450, family 21, subfamily A, polypeptide 2 (CYP21A2), OriGene unique
variant 1
TC120802 NM_000500 Homo sapiens cytochrome P450, family 21, subfamily A, polypeptide 2 (CYP21A2)
TC119671 NM_000761 Homo sapiens cytochrome P450, family 1, subfamily A, polypeptide 2 (CYP1A2)
TC119672 NM_000762 Homo sapiens cytochrome P450, family 2, subfamily A, polypeptide 6 (CYP2A6), OriGene unique variant 1
TC120818 NM_000762 Homo sapiens cytochrome P450, family 2, subfamily A, polypeptide 6 (CYP2A6)
TC120800 NM_000764 Homo sapiens cytochrome P450, family 2, subfamily A, polypeptide 7 (CYP2A7), transcript variant 1
TC124911 NM_000764 Homo sapiens cytochrome P450, family 2, subfamily A, polypeptide 7 (CYP2A7), transcript variant 1
TC110865 NM_000765 Homo sapiens cytochrome P450, family 3, subfamily A, polypeptide 7 (CYP3A7)
TC119674 NM_000767 Homo sapiens cytochrome P450, family 2, subfamily B, polypeptide 6 (CYP2B6)
TC119677 NM_000771 Homo sapiens cytochrome P450, family 2, subfamily C, polypeptide 9 (CYP2C9)
TC120796 NM_000772 Homo sapiens cytochrome P450, family 2, subfamily C, polypeptide 18 (CYP2C18)
TC119678 NM_000773 Homo sapiens cytochrome P450, family 2, subfamily E, polypeptide 1 (CYP2E1)
TC120795 NM_000774 Homo sapiens cytochrome P450, family 2, subfamily F, polypeptide 1 (CYP2F1), OriGene unique variant 1
TC120804 NM_000774 Homo sapiens cytochrome P450, family 2, subfamily F, polypeptide 1 (CYP2F1), OriGene unique variant 2
TC119679 NM_000775 Homo sapiens cytochrome P450, family 2, subfamily J, polypeptide 2 (CYP2J2)
TC120801 NM_000777 Homo sapiens cytochrome P450, family 3, subfamily A, polypeptide 5 (CYP3A5)
TC120814 NM_000778 Homo sapiens cytochrome P450, family 4, subfamily A, polypeptide 11 (CYP4A11)
TC119682 NM_000778 Homo sapiens cytochrome P450, family 4, subfamily A, polypeptide 11 (CYP4A11), OriGene unique variant 1
TC119683 NM_000779 Homo sapiens cytochrome P450, family 4, subfamily B, polypeptide 1 (CYP4B1)
TC120788 NM_000779 Homo sapiens cytochrome P450, family 4, subfamily B, polypeptide 1 (CYP4B1), OriGene unique variant 1
TC123882 NM_000780 Homo sapiens cytochrome P450, family 7, subfamily A, polypeptide 1 (CYP7A1)
TC119684 NM_000781 Homo sapiens cytochrome P450, family 11, subfamily A, polypeptide 1 (CYP11A1), nuclear gene encoding
mitochondrial protein
TC123884 NM_000783 Homo sapiens cytochrome P450, family 26, subfamily A, polypeptide 1 (CYP26A1), transcript variant 1
TC109141 NM_000783 Homo sapiens cytochrome P450, family 26, subfamily A, polypeptide 1 (CYP26A1), OriGene unique variant 1
TC123897 NM_000783 Homo sapiens cytochrome P450, family 26, subfamily A, polypeptide 1 (CYP26A1), transcript variant 1
TC119685 NM_000784 Homo sapiens cytochrome P450, family 27, subfamily A, polypeptide 1 (CYP27A1), nuclear gene encoding
mitochondrial protein
TC123873 NM_000785 Homo sapiens cytochrome P450, family 27, subfamily B, polypeptide 1 (CYP27B1), nuclear gene encoding
mitochondrial protein
TC119686 NM_000786 Homo sapiens cytochrome P450, family 51, subfamily A, polypeptide 1 (CYP51A1)
TC119595 NM_000896 Homo sapiens cytochrome P450, family 4, subfamily F, polypeptide 3 (CYP4F3)
TC120817 NM_000917 Homo sapiens procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha
polypeptide I (P4HA1), OriGene unique variant 1
TC110967 NM_000917 Homo sapiens procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha
polypeptide I (P4HA1)
TC100401 NM_000941 Homo sapiens P450 (cytochrome) oxidoreductase (POR)
TC123875 NM_000961 Homo sapiens prostaglandin I2 (prostacyclin) synthase (PTGIS)
TC109823 NM_001061 Homo sapiens thromboxane A synthase 1 (platelet, cytochrome P450, family 5, subfamily A) (TBXAS1),
transcript variant TXS-I
TC120807 NM_001082 Homo sapiens cytochrome P450, family 4, subfamily F, polypeptide 2 (CYP4F2)
TC108802 NM_004391 Homo sapiens cytochrome P450, family 8, subfamily B, polypeptide 1 (CYP8B1)
TC117125 NM_004820 Homo sapiens cytochrome P450, family 7, subfamily B, polypeptide 1 (CYP7B1)
TC120806 NM_006668 Homo sapiens cytochrome P450, family 46, subfamily A, polypeptide 1 (CYP46A1)
TC122753 NM_006668 Homo sapiens cytochrome P450, family 46, subfamily A, polypeptide 1 (CYP46A1)
TC123877 NM_007253 Homo sapiens cytochrome P450, family 4, subfamily F, polypeptide 8 (CYP4F8)
TC120812 NM_007253 Homo sapiens cytochrome P450, family 4, subfamily F, polypeptide 8 (CYP4F8)
TC123885 NM_007253 Homo sapiens cytochrome P450, family 4, subfamily F, polypeptide 8 (CYP4F8), OriGene unique variant 1
Tru
eClo
ne™
Clon
eSet
:Gen
e-fa
mily
base
dco
llect
ion
Cat. No. Accession No. Description
TC114150 NM_016593 Homo sapiens cytochrome P450, family 39, subfamily A, polypeptide 1 (CYP39A1)
TC120794 NM_017460 Homo sapiens cytochrome P450, family 3, subfamily A, polypeptide 4 (CYP3A4), transcript variant 1
TC120787 NM_017781 Homo sapiens cytochrome P450, family 2, subfamily W, polypeptide 1 (CYP2W1)
TC120799 NM_019885 Homo sapiens cytochrome P450, family 26, subfamily B, polypeptide 1 (CYP26B1)
TC120809 NM_019885 Homo sapiens cytochrome P450, family 26, subfamily B, polypeptide 1 (CYP26B1), OriGene unique variant 1
TC112952 NM_021187 Homo sapiens cytochrome P450, family 4, subfamily F, polypeptide 11 (CYP4F11)
TC120808 NM_023944 Homo sapiens cytochrome P450, family 4, subfamily F, polypeptide 12 (CYP4F12)
TC122941 NM_023944 Homo sapiens cytochrome P450, family 4, subfamily F, polypeptide 12 (CYP4F12)
TC120811 NM_024514 Homo sapiens cytochrome P450, family 2, subfamily R, polypeptide 1 (CYP2R1)
TC109968 NM_030622 Homo sapiens cytochrome P450, family 2, subfamily S, polypeptide 1 (CYP2S1), OriGene unique variant 1
TC109139 NM_030878 Homo sapiens cytochrome P450, family 2, subfamily C, polypeptide 8 (CYP2C8), OriGene unique
variant 1p1-2
TC120816 NM_030878 Homo sapiens cytochrome P450, family 2, subfamily C, polypeptide 8 (CYP2C8), transcript variant Hp1-2
TC107980 NM_031226 Homo sapiens cytochrome P450, family 19, subfamily A, polypeptide 1 (CYP19A1), transcript variant 2
TC107001 NM_177538 Homo sapiens cytochrome P450, family 20, subfamily A, polypeptide 1 (CYP20A1), transcript variant 1
TC107078 NM_178033 Homo sapiens cytochrome P450, family 4, subfamily X, polypeptide 1 (CYP4X1), OriGene unique variant 1
TC120798 NM_178033 Homo sapiens cytochrome P450, family 4, subfamily X, polypeptide 1 (CYP4X1)
TC123874 NM_178134 Homo sapiens cytochrome P450, family 4, subfamily Z, polypeptide 1 (CYP4Z1)
TC120792 NM_183075 Homo sapiens cytochrome P450, family 2, subfamily U, polypeptide 1 (CYP2U1)
TC106908 NR_001278 Homo sapiens cytochrome P450, family 2, subfamily B, polypeptide 7 pseudogene 1 (CYP2B7P1) on
chromosome 19
TC120791 XM_208213 PREDICTED: Homo sapiens cytochrome P450, family 4, subfamily A, polypeptide 22 (CYP4A22)
TC120790 XM_209612 Homo sapiens hypothetical protein LOC285440 (LOC285440)
TC123886 XM_291005 Homo sapiens similar to 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial precursor
(Calcidiol 1-monooxygenase) (25-OHD-1 alpha-hydroxylase) (25-hydroxyvitamin D(3) 1-alpha-hydroxylase)
(VD3 1A hydroxylase) (P450C1 alpha) (P450VD1-alpha) (LOC339761)
43ph: 1.888.267.4436 fax: 1.301.340.9254
44 www.origene.com
HuSH™Premade shRNA expression panels
Kinase shRNAs
Nuclear hormone receptor shRNAs
Signal molecule shRNAs
pRS vectors
1. Unlocking the potential of the human genome with RNA interference.Nature. 2004 Sep 16;431(7006):371-8.
2. RNAi: double-stranded RNA directs the ATP-dependent cleavage of mRNA at 21 to 23nucleotide intervals.Cell. 2000 Mar 31;101(1):25-33.
3. siRNAs: applications in functional genomics and potential as therapeutics.Nat Rev Drug Discov. 2004 Apr;3(4):318-29.
4. Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells.Nature. 2001 May 24;411(6836):494-8.
5. A system for stable expression of short interfering RNAs in mammalian cells.Science. 2002 Apr 19;296(5567):550-3.
6. Short hairpin RNAs (shRNAs) induce sequence-specific silencing in mammalian cells.Genes Dev. 2002 Apr 15;16(8):948-58.
Hu
SH
™Pr
emad
esh
RNA
expr
essi
onpa
nels
46 www.origene.com
Features Plasmid based vectors Synthetic siRNA
Antibiotic resistance for selection Yes No
Monitor cell transfection efficiency Yes No
Cost per gene Moderate High
Gene inhibition studies Long term Short term
Stable cell line production Yes No
Effective delivery of siRNA into hard to transfect cells Yes No
Introduction:
As a cellular defense mechanism, host cells process
double-stranded RNA into small interfering RNA
(siRNA), which then targets homologous RNAs for
destruction1. RNAi occurs through a series of steps
involving the generation of small interfering RNAs
(siRNAs) in vivo via the action of a specific RNAase III
endonuclease Dicer.The resulting siRNAs mediate the
degradation of their complementary RNA by
association of the siRNA with a nuclease complex to
form what is called the RNA-Induced Silencing
Complex (RISC). In the next step, an unwinding of the
siRNA occurs which activates RISC. It is the activated
RISC that binds to the target mRNA and finally leads
to the loss of expression of the gene it coded2.
RNAi technology can be implemented mainly with
either small interference double stranded RNA
(siRNA) or short hairpin RNA (shRNA). Experiments
have indicated that both siRNA and shRNA are also
far more potent than hammerhead ribosome
ribozymes based approaches3. In mammalian cells,
RNA interference (RNAi) can be triggered by 21-
base-pair siRNA that causes strong, yet transient,
inhibition of gene expression on specific genes4.
These siRNAs can be generated in vitro and
transfected into mammalian cells, resulting in
effective suppression of the intended genes.
Unfortunately, such suppression is typically
transient. By contrast, vector-expressing short
hairpin RNA (shRNA) can suppress gene expression
over a prolonged period(5,6). The unique advantages
of the plasmid-based shRNA approach are listed
below.
HuSH™—Premade shRNA
expression panels
Product Listing Cat. No. PriceHuSH™ shRNA panels TRXXXXXX 380
Hu
SH
™Pr
emad
esh
RNA
expr
essi
onpa
nels
47ph: 1.888.267.4436 fax: 1.301.340.9254
Product Description
What are HuSH™ shRNA expression plasmids?HuSH plasmids were designed to effectively express
the insert DNA sequences in transfected mammalian
cells. OriGene gene-specific silencing plasmids
contain a U6 promoter, 21 base pair (bp) gene-
specific sequences, a 10 bp intervening sequence,
another 21 base pair gene-specific reverse
complement sequence, and a transcription termi-
nation sequence. The expression of the insert
sequence in mammalian cells results in the
formation of a short hairpin RNA with a 21 bp stem
and a 10-nucleotide loop. Such a structure has been
shown to be able to effectively knock-down the
expression of target genes.
OriGene’s HuSH plasmids have been shown to
knock-down the expression of green fluorescence
protein, luciferase and HER2 oncogene. On
average, more than 25% of the shRNA tested are
capable of inhibiting the target genes by 70% or
more. In addition, the pRS plasmid contains a
puromycin selectable marker, and a pair of retro-
viral integration sites flanking the gene silencing
cassette and the selection marker. Both the
selection marker and the retroviral integration
functions have been validated.
HuSH plasmids utilize the most commonly used
human U6 promoter to express the shRNA. A poly-T
track is added immediately downstream of the hairpin
sequence to ensure the termination of transcription
following the hairpin sequences. The resulting shRNA
is to be processed by Dicers, and incorporated into
RISC complexes. Once in the RISC complexes, they
will find the target mRNA through specific sequence
recognition and cleave the target mRNA.
With the HuSH shRNA expression vectors, shRNA
(short-hairpin RNA) can be expressed continuously
in human cells to achieve stable and reliable gene
ADVANTAGES
Ready-to-transfect constructs
Multiple vectors to achieve optimized/dosed inhibition
PuromycinR marker for selection
Enable stable cell line production
More effective delivery
Non-exhaustible supply of DNA
shRNA oligos
pBS Ori
pRS5522 bp
U6 promoter
Ampicillin
SV40 early promoter
Puro
3‘ LTR5‘ LTR
BamH I
Not I
Hind IIIBbs I
Sal I
Cla I
Eco R I
Hu
SH
™Pr
emad
esh
RNA
expr
essi
onpa
nels
48 www.origene.com
silencing. By establishing stable cell lines with
effective shRNA expression vectors, one can perform
long term gene silencing studies, eliminating the
need to synthesize siRNA, repeat transfection, and
validate, as well as eliminating the experimental
variation frequently associated with siRNA.
The HuSH backbone pRS vector (Fig. 1):
• Designed for long-term gene silencing studies
• MMLV LTR sequences for establishing stable cell
lines via retroviruses
• Puromycin resistant marker for easy selection of
infected or transfected cells
• U6 polymerase III promoter for shRNA expression
Why should I buy a HuSH mammalianshRNA expression plasmid rather than a synthetic siRNA?
HuSH plasmids offer researchers an alternative to
inhibiting target genes. There are several advantages
to this expression plasmid based approach:
1. Long-term gene silencing:
The length of gene silencing can be longer as the
plasmid continuously expresses the shRNA of the
plasmid. In contrast, synthetic siRNAs either get
diluted during cell division or are degraded.
2. Drug-selectivity:
When the transfection efficiency is limited, one can
select plasmid-transfected cells with puromycin to
ensure efficient gene silencing.
3. Retroviral capacity:
One can obtain stable cell lines either through plas-
mid transfection or viral infection via a packaging
cell line.
4. Inexhaustible supply:
The plasmids can be amplified and thus represent
an unlimited source for future experiments.
What is the U6 promoter?
The pRS plasmid contains the human U6 promoter
that has been used extensively to express shRNAs in
mammalian cells through RNA Poll III. This promoter
Fig. 1
pRS plasmid map. The shRNA oligos will be cloned between
BamHI and BbsI, immediately downstream of U6 promoter. A
puromycin selection marker is present. Both 5’ and 3’ LTRs are
from MMLV and used to generate retroviruses in appropriate
packaging cells.
Hu
SH
™Pr
emad
esh
RNA
expr
essi
onpa
nels
49ph: 1.888.267.4436 fax: 1.301.340.9254
has been validated in the HuSH plasmid via gene
knock-down experiments.
What is the gene specific shDNA inserted downstream of U6 promoter?The shDNA inserts were generated directly from the
cDNA of the target genes through a random
restriction enzyme digestion approach. All shRNA
expression cassette sequences were verified via
DNA sequence analysis and matched against the
target gene sequences. Both the sequences and the
corresponding locations of the target genes are
provided.
How does the transcription terminate?A stretch of 5 thymidine nucleotides (dT5) is attached
immediately after the shRNA sequences. This dT5
leads to transcription termination immediately
following the shRNA sequences.
How does OriGene verify the shDNAsequences for specific genes?All shRNA sequences in the expression vectors were
verified through DNA sequence analysis and
matched against the target genes.
Why does OriGene provide up to 8 shRNA expression vectors per gene?Based on experiments conducted at OriGene,
approximately 25-30% of the shRNA vectors resulted
in >70% inhibition of the target genes. Testing 8
shRNA expression vectors virtually ensures that one
can obtain at least one functional shRNA vector.
How should I use the products?OriGene recommends that researchers first transfect
an aliquot of each of the supplied plasmids into
competent E. coli cells and perform a DNA mini-prep.
The plasmids can then be transfected into a target
cell line with FuGene6. Three days after transfection,
cell lysates can be obtained and used for western
blot analysis using an antibody against the target
protein to verify the functionality of the shRNA
vectors.
How do I choose the right shRNAexpression vector for gene silencing?The functionally active shRNA expression plasmids
need to be selected experimentally in transiently
transfected cells.
Can I select for the pRS plasmidtransfected cells?Yes. Two days after transfection, the cells can be
removed from the plates via trypsin, plated and
selected out with culture media containing
puromycin (0.5 ug/ml).
How can I create a stable cell line with afunctional shRNA expression vector? Stable cell lines can be generated using two different
approaches. First, target HEK cells can be transfected
with a functionally validated shRNA plasmid. Two
days after transfection, the transfected HEK cells can
be selected with 0.5 ug/ml puromycin over one week
with passages as needed. Alternatively, retroviral
packaging cell lines can be used to generate
retroviruses for stable cell line generation. In one
example, the plasmid can be transiently transfected
into a Phoenix helper free amphotropic packaging
cells line (http://www.orbigen.com/). Two days after
transfection, the cell culture supernatant is collected,
filtered, and used to infect the target cell lines. Stable
cell lines can be established following drug selection.
For what purpose is the empty pRS-Con used?For shRNA experiments, it is important to demonstrate
that the effect of a specific shRNA expression vector is
gene specific, and is not due to the non-specific effect
known as interferon response. pRS-Con was designed
for this purpose; the inserted cassette contains a
sequence from green fluorescence protein, unrelated
to any human expressed sequence.
What does OriGene guarantee with re-gards to its shRNA expression plasmids?OriGene guarantees all shRNA expression cassettes
are sequence verified. The pRS expression plasmid
Hu
SH
™Pr
emad
esh
RNA
expr
essi
onpa
nels
50 www.origene.com
vectors have been tested for producing shRNA in
gene silencing studies against green fluorescence
protein, luciferase and human HER2 oncogene.
Product Application:
HuSH plasmids:
Currently HuSH products are focused on
• Protein kinases
• Nuclear hormone receptors
• Signal molecules
For all HuSH products, 3-8 constructs are provided as a
panel for each target gene. The multiplicity of the vec-
tors is to ensure optimized inhibited gene expression.
As the shRNA constructs are only sequence verified, it
is strongly recommended that users verify the func-
tions of the shRNA expression constructs in transient
experiments and select the appropriate constructs to
use either in transient studies or in generating stable
cell lines for long-term gene silencing.
While it is possible that stable cell lines can be
generated via plasmid transfection followed by
puromycin drug selection, it is highly recommended
that a retrovirual packaging cell line is used to generate
retroviruses containing the shRNA hairpin expression
cassette and to infect the target cells. It is much more
likely that the shRNA will be expressed if retroviruses
are used in generating the stable cell lines.
Application of HuSH products is illustrated in Fig. 2.
HuSH products can be searched on OriGene’s
website http://www.origene.com/rna/ based on the
properties of the target genes. The search criteria are:
• Nucleotide Sequence, Protein Sequence
• Accession or Catalog Number
• Keywords
• Gene Family
Fig. 2
Cat# GeneID/LocusID description price
TR200002 90 HuSH ACVR1 shRNA Constructs against activin A receptor, type I 380
TR200003 91 HuSH ACVR1B shRNA Constructs against activin A receptor, type IB 380
TR200004 157 HuSH ADRBK2 shRNA Constructs against adrenergic, beta, receptor kinase 2 ) 380
TR200005 207 HuSH AKT1 shRNA Constructs against v-akt murine thymoma viral oncogene homolog 1 380
TR200006 369 HuSH ARAF1 shRNA Constructs against v-raf murine sarcoma 3611 viral oncogene homolog 1 380
TR200007 659 HuSH BMPR2 shRNA Constructs against bone morphogenetic protein receptor, type II (serine/threonine kinase) 380
TR200008 660 HuSH BMX shRNA Constructs against BMX non-receptor tyrosine kinase 380
TR200009 676 HuSH BRDT shRNA Constructs against bromodomain, testis-specific 380
TR200010 695 HuSH BTK shRNA Constructs against Bruton agammaglobulinemia tyrosine kinase 380
TR200011 780 HuSH DDR1 shRNA Constructs against discoidin domain receptor family, member 1 380
TR200012 815 HuSH CAMK2A shRNA Constructs against calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha 380
TR200013 818 HuSH CAMK2G shRNA Constructs against calcium/calmodulin-dependent protein kinase (CaM kinase) II gamma 380
TR200014 984 HuSH CDC2L1 shRNA Constructs against cell division cycle 2-like 1 (PITSLRE proteins) 380
TR200015 1017 HuSH CDK2 shRNA Constructs against cyclin-dependent kinase 2 380
TR200016 1018 HuSH CDK3 shRNA Constructs against cyclin-dependent kinase 3 380
TR200017 1019 HuSH CDK4 shRNA Constructs against cyclin-dependent kinase 4 380
TR200018 1020 HuSH CDK5 shRNA Constructs against cyclin-dependent kinase 5 380
TR200019 1022 HuSH CDK7 shRNA Constructs against cyclin-dependent kinase 7 (MO15 homolog, Xenopus laevis, cdk- 380
activating kinase)
TR200020 1111 HuSH CHEK1 shRNA Constructs against CHK1 checkpoint homolog (S. pombe) 380
TR200021 1195 HuSH CLK1 shRNA Constructs against CDC-like kinase 1 380
TR200022 1198 HuSH CLK3 shRNA Constructs against CDC-like kinase 3 380
TR200023 1326 HuSH MAP3K8 shRNA Constructs against mitogen-activated protein kinase kinase kinase 8 380
TR200024 1436 HuSH CSF1R shRNA Constructs against colony stimulating factor 1 receptor, formerly McDonough feline 380
sarcoma viral (v-fms) oncogene homolog
TR200025 1452 HuSH CSNK1A1 shRNA Constructs against casein kinase 1, alpha 1 380
TR200026 1453 HuSH CSNK1D shRNA Constructs against casein kinase 1, delta 380
TR200027 1455 HuSH CSNK1G2 shRNA Constructs against casein kinase 1, gamma 2 380
TR200028 1456 HuSH CSNK1G3 shRNA Constructs against casein kinase 1, gamma 3 380
TR200029 1457 HuSH CSNK2A1 shRNA Constructs against casein kinase 2, alpha 1 polypeptide 380
TR200030 1760 HuSH DMPK shRNA Constructs against dystrophia myotonica-protein kinase 380
TR200031 2041 HuSH EPHA1 shRNA Constructs against erythropoietin-producing hepatoma A1 380
TR200032 2042 HuSH EPHA3 shRNA Constructs against erythropoietin-producing hepatoma A3 380
TR200033 2043 HuSH EPHA4 shRNA Constructs against erythropoietin-producing hepatoma A4 380
TR200034 2044 HuSH EPHA5 shRNA Constructs against erythropoietin-producing hepatoma A5 380
TR200035 2050 HuSH EPHB4 shRNA Constructs against erythropoietin-producing hepatoma B4 380
TR200036 2051 HuSH EPHB6 shRNA Constructs against erythropoietin-producing hepatoma B6 380
TR200037 2064 HuSH ERBB2 shRNA Constructs against v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, 380
neuro/glioblastoma derived oncogene homolog (avian)
TR200038 2065 HuSH ERBB3 shRNA Constructs against v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian) 380
TR200039 2185 HuSH PTK2B shRNA Constructs against PTK2B protein tyrosine kinase 2 beta 380
Hu
SH
™Pr
emad
esh
RNA
expr
essi
onpa
nels
51ph: 1.888.267.4436 fax: 1.301.340.9254
HuSH™—Premade shRNA expression panels
Product offering is subject to change. Refer to www.origene.com for the most updated information.
Hu
SH
™Pr
emad
esh
RNA
expr
essi
onpa
nels
Cat# GeneID/LocusID description price
TR200040 2242 HuSH FES shRNA Constructs against feline sarcoma oncogene 380
TR200041 2260 HuSH FGFR1 shRNA Constructs against fibroblast growth factor receptor 1 (fms-related tyrosine kinase 2, 380
Pfeiffer syndrome)
TR200042 2264 HuSH FGFR4 shRNA Constructs against fibroblast growth factor receptor 4 380
TR200043 2268 HuSH FGR shRNA Constructs against Gardner-Rasheed feline sarcoma viral (v-fgr) oncogene homolog 380
TR200044 2324 HuSH FLT4 shRNA Constructs against fms-related tyrosine kinase 4 380
TR200045 2475 HuSH FRAP1 shRNA Constructs against FK506 binding protein 12-rapamycin associated protein 1 380
TR200046 2534 HuSH FYN shRNA Constructs against FYN oncogene related to SRC, FGR, YES 380
TR200047 2869 HuSH GPRK5 shRNA Constructs against G protein-coupled receptor kinase 5 380
TR200048 2872 HuSH MKNK2 shRNA Constructs against MAP kinase interacting serine/threonine kinase 2 380
TR200049 2932 HuSH GSK3B shRNA Constructs against glycogen synthase kinase 3 beta 380
TR200050 3055 HuSH HCK shRNA Constructs against hemopoietic cell kinase 380
TR200051 3551 HuSH IKBKB shRNA Constructs against inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta 380
TR200052 3611 HuSH ILK shRNA Constructs against integrin-linked kinase 380
TR200053 3656 HuSH IRAK2 shRNA Constructs against interleukin-1 receptor-associated kinase 2 380
TR200054 3702 HuSH ITK shRNA Constructs against IL2-inducible T-cell kinase 380
TR200055 3716 HuSH JAK1 shRNA Constructs against Janus kinase 1 (a protein tyrosine kinase) 380
TR200056 3791 HuSH KDR shRNA Constructs against kinase insert domain receptor (a type III receptor tyrosine kinase) (KDR), 380
VEGF receptor
TR200057 3815 HuSH KIT shRNA Constructs against v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog 380
TR200058 3932 HuSH LCK shRNA Constructs against lymphocyte-specific protein tyrosine kinase 380
TR200059 3984 HuSH LIMK1 shRNA Constructs against LIM domain kinase 1 380
TR200060 3985 HuSH LIMK2 shRNA Constructs against LIM domain kinase 2 380
TR200061 4117 HuSH MAK shRNA Constructs against male germ cell-associated kinase 380
TR200062 4140 HuSH MARK3 shRNA Constructs against MAP/microtubule affinity-regulating kinase 3 380
TR200063 4638 HuSH MYLK shRNA Constructs against myosin, light polypeptide kinase 380
TR200064 4750 HuSH NEK1 shRNA Constructs against NIMA (never in mitosis gene a)-related kinase 1 380
TR200065 4751 HuSH NEK2 shRNA Constructs against NIMA (never in mitosis gene a)-related kinase 2 380
TR200066 4882 HuSH NPR2 shRNA Constructs against natriuretic peptide receptor B/guanylate cyclase B (atrionatriuretic peptide 380
receptor B)
TR200067 4916 HuSH NTRK3 shRNA Constructs against neurotrophic tyrosine kinase, receptor, type 3 380
TR200068 4921 HuSH DDR2 shRNA Constructs against discoidin domain receptor family, member 2 380
TR200069 5058 HuSH PAK1 shRNA Constructs against p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast) 380
TR200070 5063 HuSH PAK3 shRNA Constructs against p21 (CDKN1A)-activated kinase 3 380
TR200071 5127 HuSH PCTK1 shRNA Constructs against PCTAIRE protein kinase 1 380
TR200072 5164 HuSH PDK2 shRNA Constructs against pyruvate dehydrogenase kinase, isoenzyme 2 380
TR200073 5218 HuSH PFTK1 shRNA Constructs against PFTAIRE protein kinase 1 380
TR200074 5347 HuSH PLK shRNA Constructs against polo-like kinase 1 (Drosophila) 380
TR200075 5562 HuSH PRKAA1 shRNA Constructs against protein kinase, AMP-activated, alpha 1 catalytic subunit 380
TR200076 5567 HuSH PRKACB shRNA Constructs against protein kinase, cAMP-dependent, catalytic, beta 380
TR200077 5568 HuSH PRKACG shRNA Constructs against protein kinase, cAMP-dependent, catalytic, gamma 380
TR200078 5579 HuSH PRKCB1 shRNA Constructs against protein kinase C, beta 1 380
TR200079 5581 HuSH PRKCE shRNA Constructs against protein kinase C, epsilon 380
TR200080 5582 HuSH PRKCG shRNA Constructs against protein kinase C, gamma 380
TR200081 5583 HuSH PRKCH shRNA Constructs against protein kinase C, eta 380
TR200082 5588 HuSH PRKCQ shRNA Constructs against protein kinase C, theta 380
TR200083 5590 HuSH PRKCZ shRNA Constructs against protein kinase C, zeta 380
TR200084 5597 HuSH MAPK6 shRNA Constructs against mitogen-activated protein kinase 6 380
52 www.origene.com
Product offering is subject to change. Refer to www.origene.com for the most updated information.
Hu
SH
™Pr
emad
esh
RNA
expr
essi
onpa
nels
Cat# GeneID/LocusID description price
TR200085 5598 HuSH MAPK7 shRNA Constructs against mitogen-activated protein kinase 7 380
TR200086 5601 HuSH MAPK9 shRNA Constructs against mitogen-activated protein kinase 9 380
TR200087 5602 HuSH MAPK10 shRNA Constructs against mitogen-activated protein kinase 10 380
TR200088 5607 HuSH MAP2K5 shRNA Constructs against mitogen-activated protein kinase kinase 5 380
TR200089 5610 HuSH PRKR shRNA Constructs against protein kinase, interferon-inducible double stranded RNA dependent 380
TR200090 5753 HuSH PTK6 shRNA Constructs against PTK6 protein tyrosine kinase 6 380
TR200091 5756 HuSH PTK9 shRNA Constructs against PTK9 protein tyrosine kinase 9 380
TR200092 5891 HuSH RAGE shRNA Constructs against renal tumor antigen 380
TR200093 5894 HuSH RAF1 shRNA Constructs against v-raf-1 murine leukemia viral oncogene homolog 1 380
TR200094 6041 HuSH RNASEL shRNA Constructs against ribonuclease L (2’,5’-oligoisoadenylate synthetase-dependent) 380
TR200095 6046 HuSH BRD2 shRNA Constructs against bromodomain containing 2 380
TR200096 6197 HuSH RPS6KA3 shRNA Constructs against ribosomal protein S6 kinase, 90kDa, polypeptide 3 380
TR200097 6198 HuSH RPS6KB1 shRNA Constructs against ribosomal protein S6 kinase, 70kDa, polypeptide 1 380
TR200098 6199 HuSH RPS6KB2 shRNA Constructs against ribosomal protein S6 kinase, 70kDa, polypeptide 2 380
TR200099 6446 HuSH SGK shRNA Constructs against serum/glucocorticoid regulated kinase 380
TR200100 6732 HuSH SRPK1 shRNA Constructs against SFRS protein kinase 1 380
TR200101 6733 HuSH SRPK2 shRNA Constructs against SFRS protein kinase 2 380
TR200102 6787 HuSH NEK4 shRNA Constructs against NIMA (never in mitosis gene a)-related kinase 4 380
TR200103 6789 HuSH STK4 shRNA Constructs against serine/threonine kinase 4 380
TR200104 6790 HuSH STK6 shRNA Constructs against serine/threonine kinase 6 380
TR200105 6792 HuSH CDKL5 shRNA Constructs against cyclin-dependent kinase-like 5 380
TR200106 6795 HuSH AURKC shRNA Constructs against aurora kinase C 380
TR200107 6850 HuSH SYK shRNA Constructs against spleen tyrosine kinase 380
TR200108 6885 HuSH MAP3K7 shRNA Constructs against mitogen-activated protein kinase kinase kinase 7 380
TR200109 7010 HuSH TEK shRNA Constructs against TEK tyrosine kinase, endothelial (venous malformations, multiple 380
cutaneous and mucosal)
TR200110 7272 HuSH TTK shRNA Constructs against TTK protein kinase 380
TR200111 7294 HuSH TXK shRNA Constructs against TXK tyrosine kinase 380
TR200112 7297 HuSH TYK2 shRNA Constructs against tyrosine kinase 2 380
TR200113 7443 HuSH VRK1 shRNA Constructs against vaccinia related kinase 1 380
TR200114 7867 HuSH MAPKAPK3 shRNA Constructs against mitogen-activated protein kinase-activated protein kinase 3 380
TR200115 8444 HuSH DYRK3 shRNA Constructs against dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 3 380
TR200116 8476 HuSH CDC42BPA shRNA Constructs against CDC42 binding protein kinase alpha (DMPK-like) 380
TR200117 8550 HuSH MAPKAPK5 shRNA Constructs against mitogen-activated protein kinase-activated protein kinase 5 380
TR200118 8558 HuSH CDK10 shRNA Constructs against cyclin-dependent kinase (CDC2-like) 10 380
TR200119 8569 HuSH MKNK1 shRNA Constructs against MAP kinase interacting serine/threonine kinase 1 380
TR200120 8573 HuSH CASK shRNA Constructs against calcium/calmodulin-dependent serine protein kinase (MAGUK family) 380
TR200121 8767 HuSH RIPK2 shRNA Constructs against receptor-interacting serine-threonine kinase 2 380
TR200122 8780 HuSH RIOK3 shRNA Constructs against RIO kinase 3 (yeast) 380
TR200123 8859 HuSH STK19 shRNA Constructs against serine/threonine kinase 19 380
TR200124 8899 HuSH PRPF4B shRNA Constructs against PRP4 pre-mRNA processing factor 4 homolog B (yeast) 380
TR200125 9064 HuSH MAP3K6 shRNA Constructs against mitogen-activated protein kinase kinase kinase 6 380
TR200126 9175 HuSH MAP3K13 shRNA Constructs against mitogen-activated protein kinase kinase kinase 13 380
TR200127 9641 HuSH IKBKE shRNA Constructs against inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase epsilon 380
TR200128 9833 HuSH MELK shRNA Constructs against maternal embryonic leucine zipper kinase 380
TR200129 10110 HuSH SGK2 shRNA Constructs against serum/glucocorticoid regulated kinase 2 380
TR200130 10295 HuSH BCKDK shRNA Constructs against branched chain alpha-ketoacid dehydrogenase kinase 380
53ph: 1.888.267.4436 fax: 1.301.340.9254
Product offering is subject to change. Refer to www.origene.com for the most updated information.
Hu
SH
™Pr
emad
esh
RNA
expr
essi
onpa
nels
Cat# GeneID/LocusID description price
TR200131 10420 HuSH TESK2 shRNA Constructs against testis-specific kinase 2 380
TR200132 10461 HuSH MERTK shRNA Constructs against c-mer proto-oncogene tyrosine kinase 380
TR200133 10494 HuSH STK25 shRNA Constructs against serine/threonine kinase 25 (STE20 homolog, yeast) 380
TR200134 10645 HuSH CAMKK2 shRNA Constructs against calcium/calmodulin-dependent protein kinase kinase 2, beta 380
TR200135 10922 HuSH FASTK shRNA Constructs against FAST kinase 380
TR200136 11011 HuSH TLK2 shRNA Constructs against tousled-like kinase 2 380
TR200137 11040 HuSH PIM2 shRNA Constructs against pim-2 oncogene 380
TR200138 11156 HuSH PTP4A3 shRNA Constructs against protein tyrosine phosphatase type IVA, member 3 380
TR200139 11200 HuSH CHEK2 shRNA Constructs against CHK2 checkpoint homolog (S. pombe) 380
TR200140 11329 HuSH STK38 shRNA Constructs against serine/threonine kinase 38 380
TR200141 11344 HuSH PTK9L shRNA Constructs against PTK9L protein tyrosine kinase 9-like (A6-related protein) 380
TR200142 23031 HuSH KIAA0561 shRNA Constructs against PREDICTED: microtubule associated serine/threonine kinase 3 380
TR200143 23178 HuSH PASK shRNA Constructs against PAS domain containing serine/threonine kinase (PASK) 380
TR200144 23227 HuSH KIAA0303 shRNA Constructs against Predicted kinase: KIAA0303 protein (KIAA0303) 380
TR200145 23476 HuSH BRD4 shRNA Constructs against bromodomain containing 4 (BRD4) 380
TR200146 23552 HuSH CCRK shRNA Constructs against cell cycle related kinase (CCRK) 380
TR200147 25778 HuSH KIAA0472 shRNA Constructs against receptor interacting protein kinase 5 (RIPK5) 380
TR200148 25865 HuSH PRKD2 shRNA Constructs against protein kinase D2 380
TR200149 26353 HuSH H11 shRNA Constructs against heat shock 27kDa protein 8 (HSPB8) 380
TR200150 28951 HuSH TRB2 shRNA Constructs against tribbles homolog 2 (Drosophila) 380
TR200151 29941 HuSH pknbeta shRNA Constructs against protein kinase N3 (PKN3) 380
TR200152 29959 HuSH NRBP shRNA Constructs against nuclear receptor binding protein 380
TR200153 30849 HuSH PIK3R4 shRNA Constructs against phosphoinositide-3-kinase, regulatory subunit 4, p150 380
TR200154 51231 HuSH VRK3 shRNA Constructs against vaccinia related kinase 3 380
TR200155 51347 HuSH JIK shRNA Constructs against STE20-like kinase 380
TR200156 51765 HuSH MST4 shRNA Constructs against Mst3 and SOK1-related kinase 380
TR200157 54101 HuSH ANKRD3 shRNA Constructs against receptor-interacting serine-threonine kinase 4 380
TR200158 54861 HuSH SNRK shRNA Constructs against SNF-1 related kinase 380
TR200159 55351 HuSH HSA250839 shRNA Constructs against serine/threonine kinase 32B 380
TR200160 55359 HuSH DKFZp761P1010 shRNA Constructs against protein kinase STYK1 380
TR200161 55781 HuSH FLJ10074 shRNA Constructs against RIO kinase 2 (yeast) 380
TR200162 55872 HuSH RIOK2 shRNA Constructs against T-LAK cell-originated protein kinase 380
TR200163 56924 HuSH PAK6 shRNA Constructs against p21(CDKN1A)-activated kinase 6 380
TR200164 56997 HuSH CABC1 shRNA Constructs against chaperone, ABC1 activity of bc1 complex like (S. pombe) 380
TR200165 57143 HuSH ADCK1 shRNA Constructs against aarF domain containing kinase 1 380
TR200166 57144 HuSH PAK7 shRNA Constructs against p21(CDKN1A)-activated kinase 7 380
TR200167 57147 HuSH PACE-1 shRNA Constructs against ezrin-binding partner PACE-1 380
TR200168 57172 HuSH CAMK1G shRNA Constructs against calcium/calmodulin-dependent protein kinase IG 380
TR200169 57396 HuSH CLK4 shRNA Constructs against CDC-like kinase 4 380
TR200170 57410 HuSH SCYL1 shRNA Constructs against SCY1-like 1 (S. cerevisiae) 380
TR200171 57551 HuSH KIAA1361 shRNA Constructs against serine/threonine protein kinase TAO1 homolog 380
TR200172 65975 HuSH STK33 shRNA Constructs against serine/threonine kinase 33 380
TR200173 79834 HuSH KIAA2002 shRNA Constructs against Predicted kinase: KIAA2002 protein 380
TR200174 79858 HuSH NEK11 shRNA Constructs against NIMA (never in mitosis gene a)- related kinase 11 380
TR200175 79934 HuSH ADCK4 shRNA Constructs against aarF domain containing kinase 4 380
TR200176 80216 HuSH LAK shRNA Constructs against lymphocyte alpha-kinase 380
TR200177 81788 HuSH SNARK shRNA Constructs against likely ortholog of rat SNF1/AMP-activated protein kinase 380
54 www.origene.com
Product offering is subject to change. Refer to www.origene.com for the most updated information.
Hu
SH
™Pr
emad
esh
RNA
expr
essi
onpa
nels
Cat# GeneID/LocusID description price
TR200178 83694 HuSH RPS6KL1 shRNA Constructs against ribosomal protein S6 kinase-like 1 380
TR200179 83732 HuSH RIOK1 shRNA Constructs against RIO kinase 1 (yeast) 380
TR200180 84930 HuSH FLJ14813 shRNA Constructs against microtubule associated serine/threonine kinase-like 380
TR200181 85366 HuSH MYLK2 shRNA Constructs against myosin light chain kinase 2, skeletal muscle 380
TR200182 91461 HuSH LOC91461 shRNA Constructs against Predicted kinase: LOC91461 380
TR200183 92335 HuSH LYK5 shRNA Constructs against protein kinase LYK5 (LYK5) 380
TR200184 115701 HuSH HAK shRNA Constructs against heart alpha-kinase (HAK) 380
TR200185 130399 HuSH ACVR1C shRNA Constructs against activin A receptor, type IC 380
TR200186 140469 HuSH MYO3B shRNA Constructs against myosin IIIB (MYO3B) 380
TR200187 167359 HuSH MGC42105 shRNA Constructs against Predicted kinase: MGC42105 380
TR200188 197259 HuSH FLJ34389 shRNA Constructs against Predicted kinase: FLJ34389 380
TR200189 203054 HuSH ADCK5 shRNA Constructs against aarF domain containing kinase 5 380
TR200190 203447 HuSH NRK shRNA Constructs against Nik related kinase 380
TR200191 282974 HuSH STK32C shRNA Constructs against serine/threonine kinase 32C 380
TR200192 284086 HuSH NEK8 shRNA Constructs against NIMA (never in mitosis gene a)- related kinase 8 380
TR200193 340371 HuSH LOC340371 shRNA Constructs against Predicted kinase: LOC340371 380
TR200194 190 HuSH NR0B1 shRNA Constructs against nuclear receptor subfamily 0, group B, member 1 380
TR200195 238 HuSH ALK shRNA Constructs against anaplastic lymphoma kinase 380
TR200196 1789 HuSH DNMT3B shRNA Constructs against DNA (cytosine-5-)-methyltransferase 380
TR200197 1956 HuSH EGFR shRNA Constructs against epidermal growth factor receptor 380
TR200198 2100 HuSH ESR2 shRNA Constructs against estrogen receptor 2 380
TR200199 2103 HuSH ESRRB shRNA Constructs against estrogen-related receptor beta 380
TR200200 3164 HuSH NR4A1 shRNA Constructs against nuclear receptor subfamily 4, group A, member 1 380
TR200201 4929 HuSH NR4A2 shRNA Constructs against nuclear receptor subfamily 4, group A, member 2 380
TR200202 5469 HuSH PPARBP shRNA Constructs against PPAR binding protein 380
TR200203 5747 HuSH PTK2 shRNA Constructs against PTK2 protein tyrosine kinase 2 380
TR200204 6096 HuSH RORB shRNA Constructs against RAR-related orphan receptor B 380
TR200205 7157 HuSH TP53 shRNA Constructs against tumor protein p53 (Li-Fraumeni syndrome) 380
TR200206 7182 HuSH NR2C2 shRNA Constructs against nuclear receptor subfamily 2, group C, member 2 380
TR200207 7376 HuSH NR1H2 shRNA Constructs against nuclear receptor subfamily 1, group H, member 2 380
TR200208 8856 HuSH NR1I2 shRNA Constructs against nuclear receptor subfamily 1, group I, member 2 380
TR200209 9572 HuSH NR1D1 shRNA Constructs against nuclear receptor subfamily 1, group D, member 1 380
TR200210 9886 HuSH RHOBTB1 shRNA Constructs against Rho-related BTB domain containing 380
TR200211 10062 HuSH NR1H3 shRNA Constructs against nuclear receptor subfamily 1, group H, member 3 380
TR200212 29904 HuSH EEF2K shRNA Constructs against elongation factor-2 kinase 380
TR200213 23 HuSH ABCF1 shRNA Constructs against ATP-binding cassette, sub-family F (GCN20), member 1 380
TR200214 2066 HuSH ERBB4 shRNA Constructs against v-erb-a erythroblastic leukemia viral oncogene homolog 4 380
TR200215 2908 HuSH NR3C1 shRNA Constructs against nuclear receptor subfamily 3, group C, member 1 380
TR200216 4486 HuSH MST1R shRNA Constructs against macrophage stimulating 1 receptor 380
TR200217 5465 HuSH PPARA shRNA Constructs against peroxisome proliferative activated receptor, alpha 380
TR200218 5663 HuSH PSEN1 shRNA Constructs against presenilin 1 (Alzheimer disease 3) 380
TR200219 5728 HuSH PTEN shRNA Constructs against phosphatase and tensin homolog (mutated in multiple advanced cancers 1) 380
TR200220 5915 HuSH RARB shRNA Constructs against retinoic acid receptor, beta 380
TR200221 5919 HuSH RARRES2 shRNA Constructs against retinoic acid receptor responder 2 380
TR200222 5920 HuSH RARRES3 shRNA Constructs against retinoic acid receptor responder 3 380
TR200223 6258 HuSH RXRG shRNA Constructs against retinoid X receptor, gamma 380
TR200224 7098 HuSH TLR3 shRNA Constructs against toll-like receptor 3 380
55ph: 1.888.267.4436 fax: 1.301.340.9254
Product offering is subject to change. Refer to www.origene.com for the most updated information.
Hu
SH
™Pr
emad
esh
RNA
expr
essi
onpa
nels
Cat# GeneID/LocusID description price
TR200225 9252 HuSH RPS6KA5 shRNA Constructs against ribosomal protein S6 kinase, 90kDa 380
TR200226 9325 HuSH TRIP4 shRNA Constructs against thyroid hormone receptor interactor 4 380
TR200227 9971 HuSH NR1H4 shRNA Constructs against nuclear receptor subfamily 1, group H, member 4 380
TR200228 63925 HuSH ZNF335 shRNA Constructs against zinc finger protein 335 380
TR200229 102 HuSH ADAM10 shRNA Constructs against a disintegrin and metalloproteinase domain 10 380
TR200230 1432 HuSH MAPK14 shRNA Constructs against mitogen-activated protein kinase 1 380
TR200231 1969 HuSH EPHA2 shRNA Constructs against EphA2 380
TR200232 3091 HuSH HIF1A shRNA Constructs against hypoxia-inducible factor 1, alpha subunit (basic helix-loop-helix 380
transcription factor)
TR200233 3172 HuSH HNF4A shRNA Constructs against hepatocyte nuclear factor 4, alpha 380
TR200234 4292 HuSH MLH1 shRNA Constructs against mutL homolog 1 colon cancer 380
TR200235 4306 HuSH NR3C2 shRNA Constructs against nuclear receptor subfamily 3, group C, member 2 380
TR200236 5468 HuSH PPARG shRNA Constructs against peroxisome proliferative activated receptor, gamma 380
TR200237 7097 HuSH LRTLR2 shRNA Constructs against toll-like receptor 2 380
TR200238 8202 HuSH NCOA3 shRNA Constructs against nuclear receptor coactivator 3 380
TR200239 8648 HuSH NCOA1 shRNA Constructs against nuclear receptor coactivator 1 380
TR200241 9975 HuSH NR1D2 shRNA Constructs against nuclear receptor subfamily 1, group D, member 2 380
TR200242 79705 HuSH LRRK1 shRNA Constructs against leucine-rich repeat kinase 1 380
TR200243 2261 HuSH FGFR3 shRNA Constructs against fibroblast growth factor receptor 3 380
TR200244 4593 HuSH MUSK shRNA Constructs against muscle, skeletal, receptor tyrosine kinase 380
TR200245 5916 HuSH RARG shRNA Constructs against retinoic acid receptor, gamma 380
TR200246 7006 HuSH TEC shRNA Constructs against tec protein tyrosine kinase 380
TR200247 7067 HuSH THRA shRNA Constructs against thyroid hormone receptor, alpha 380
TR200248 7068 HuSH THRB shRNA Constructs against thyroid hormone receptor, beta 380
TR200249 7099 HuSH TLR4 shRNA Constructs against toll-like receptor 4 380
TR200250 7181 HuSH NR2C1 shRNA Constructs against nuclear receptor subfamily 2, group C, member 1 380
TR200251 8576 HuSH STK16 shRNA Constructs against serine/threonine kinase 16 380
TR200252 51135 HuSH IRAK4 shRNA Constructs against interleukin-1 receptor-associated kinase 4 380
TR200253 149420 HuSH LOC149420 shRNA Constructs against casein kinase 380
TR200254 156 HuSH ADRBK1 Constructs against adrenergic, beta, receptor kinase 1 380
TR200255 208 HuSH AKT2 Constructs against v-akt murine thymoma viral oncogene homolog 2 380
TR200256 351 HuSH APP Constructs against amyloid beta (A4) precursor protein (protease nexin-II, Alzheimer disease) 380
TR200257 367 HuSH AR Constructs against androgen receptor (dihydrotestosterone receptor; testicular feminization; 380
spinal and bulbar muscular atrophy; Kennedy disea
TR200258 816 HuSH CAMK2B Constructs against calcium/calmodulin-dependent protein kinase (CaM kinase) II beta 380
TR200259 896 HuSH CCND3 Constructs against cyclin D3 380
TR200260 898 HuSH CCNE1 Constructs against cyclin E1 380
TR200261 1025 HuSH CDK9 Constructs against cyclin-dependent kinase 9 (CDC2-related kinase) 380
TR200262 1030 HuSH CDKN2B Constructs against cyclin-dependent kinase inhibitor 2B (p15, inhibits CDK4) 380
TR200263 1032 HuSH CDKN2D Constructs against cyclin-dependent kinase inhibitor 2D (p19, inhibits CDK4) 380
TR200264 1263 HuSH PLK3 Constructs against polo-like kinase 3 (Drosophila) 380
TR200265 1454 HuSH CSNK1E Constructs against casein kinase 1, epsilon 380
TR200266 1611 HuSH DAP Constructs against death-associated protein 380
TR200267 1612 HuSH DAPK1 Constructs against death-associated protein kinase 1 380
TR200268 1613 HuSH DAPK3 Constructs against death-associated protein kinase 3 380
TR200269 1725 HuSH DHPS Constructs against deoxyhypusine synthase 380
TR200270 1869 HuSH E2F1 Constructs against E2F transcription factor 1 380
56 www.origene.com
Product offering is subject to change. Refer to www.origene.com for the most updated information.
Hu
SH
™Pr
emad
esh
RNA
expr
essi
onpa
nels
Cat# GeneID/LocusID description price
TR200271 2002 HuSH ELK1 Constructs against ELK1, member of ETS oncogene family 380
TR200272 2011 HuSH MARK2 Constructs against MAP/microtubule affinity-regulating kinase 2 380
TR200273 2045 HuSH EPHA7 Constructs against EPH receptor A7 380
TR200274 2046 HuSH EPHA8 Constructs against EPH receptor A8 380
TR200275 2047 HuSH EPHB1 Constructs against EPH receptor B1 380
TR200276 2049 HuSH EPHB3 Constructs against EPH receptor B3 380
TR200277 2057 HuSH EPOR Constructs against erythropoietin receptor 380
TR200278 2099 HuSH ESR1 Constructs against estrogen receptor 1 380
TR200279 2101 HuSH ESRRA Constructs against estrogen-related receptor alpha 380
TR200280 2114 HuSH ETS2 Constructs against v-ets erythroblastosis virus E26 oncogene homolog 2 (avian) 380
TR200281 2263 HuSH FGFR2 Constructs against fibroblast growth factor receptor 2 (bacteria-expressed kinase, keratinocyte 380
growth factor receptor, craniofacial dysost
TR200282 2308 HuSH FOXO1A Constructs against forkhead box O1A (rhabdomyosarcoma) 380
TR200283 2580 HuSH GAK Constructs against cyclin G associated kinase 380
TR200284 2868 HuSH GRK4 Constructs against G protein-coupled receptor kinase 4 380
TR200285 2870 HuSH GRK6 Constructs against G protein-coupled receptor kinase 6 380
TR200286 2891 HuSH GRIA2 Constructs against glutamate receptor, ionotropic, AMPA 2 380
TR200287 2931 HuSH GSK3A Constructs against glycogen synthase kinase 3 alpha 380
TR200288 3315 HuSH HSPB1 Constructs against heat shock 27kDa protein 1 380
TR200289 3480 HuSH IGF1R Constructs against insulin-like growth factor 1 receptor 380
TR200290 3645 HuSH INSRR Constructs against insulin receptor-related receptor 380
TR200291 3654 HuSH IRAK1 Constructs against interleukin-1 receptor-associated kinase 1 380
TR200292 3725 HuSH JUN Constructs against v-jun sarcoma virus 17 oncogene homolog (avian) 380
TR200293 3726 HuSH JUNB Constructs against jun B proto-oncogene 380
TR200294 4041 HuSH LRP5 Constructs against low density lipoprotein receptor-related protein 5 380
TR200295 4086 HuSH SMAD1 Constructs against SMAD, mothers against DPP homolog 1 (Drosophila) 380
TR200296 4092 HuSH SMAD7 Constructs against SMAD, mothers against DPP homolog 7 (Drosophila) 380
TR200297 4094 HuSH MAF Constructs against v-maf musculoaponeurotic fibrosarcoma oncogene homolog (avian) 380
TR200298 4139 HuSH MARK1 Constructs against MAP/microtubule affinity-regulating kinase 1 380
TR200299 4217 HuSH MAP3K5 Constructs against mitogen-activated protein kinase kinase kinase 5 380
TR200300 4296 HuSH MAP3K11 Constructs against mitogen-activated protein kinase kinase kinase 11 380
TR200301 4790 HuSH NFKB1 Constructs against nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 (p105) 380
TR200302 4793 HuSH NFKBIB Constructs against nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, beta 380
TR200303 4846 HuSH NOS3 Constructs against nitric oxide synthase 3 (endothelial cell) 380
TR200304 4915 HuSH NTRK2 Constructs against neurotrophic tyrosine kinase, receptor, type 2 380
TR200305 4919 HuSH ROR1 Constructs against receptor tyrosine kinase-like orphan receptor 1 380
TR200306 5128 HuSH PCTK2 Constructs against PCTAIRE protein kinase 2 380
TR200307 5170 HuSH PDPK1 Constructs against 3-phosphoinositide dependent protein kinase-1 380
TR200308 5184 HuSH PEPD Constructs against peptidase D 380
TR200309 5241 HuSH PGR Constructs against progesterone receptor 380
TR200310 5467 HuSH PPARD Constructs against peroxisome proliferative activated receptor, delta 380
TR200311 5585 HuSH PKN1 Constructs against protein kinase N1 380
TR200312 5586 HuSH PKN2 Constructs against protein kinase N2 380
TR200313 5587 HuSH PRKD1 Constructs against protein kinase D1 380
TR200314 5604 HuSH MAP2K1 Constructs against mitogen-activated protein kinase kinase 1 380
TR200315 5605 HuSH MAP2K2 Constructs against mitogen-activated protein kinase kinase 2 380
TR200316 5606 HuSH MAP2K3 Constructs against mitogen-activated protein kinase kinase 3 380
57ph: 1.888.267.4436 fax: 1.301.340.9254
Product offering is subject to change. Refer to www.origene.com for the most updated information.
Hu
SH
™Pr
emad
esh
RNA
expr
essi
onpa
nels
Cat# GeneID/LocusID description price
TR200317 5918 HuSH RARRES1 Constructs against retinoic acid receptor responder (tazarotene induced) 1 380
TR200318 5925 HuSH RB1 Constructs against retinoblastoma 1 (including osteosarcoma) 380
TR200319 5979 HuSH RET Constructs against ret proto-oncogene (multiple endocrine neoplasia and medullary thyroid carcinoma 1,
Hirschsprung disease) 380
TR200320 6095 HuSH RORA Constructs against RAR-related orphan receptor A 380
TR200321 6195 HuSH RPS6KA1 Constructs against ribosomal protein S6 kinase, 90kDa, polypeptide 1 380
TR200322 6256 HuSH RXRA Constructs against retinoid X receptor, alpha 380
TR200323 6257 HuSH RXRB Constructs against retinoid X receptor, beta 380
TR200324 6259 HuSH RYK Constructs against RYK receptor-like tyrosine kinase 380
TR200325 6300 HuSH MAPK12 Constructs against mitogen-activated protein kinase 12 380
TR200326 6714 HuSH SRC Constructs against v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) 380
TR200327 6722 HuSH SRF Constructs against serum response factor (c-fos serum response element-binding transcription factor) 380
TR200328 6776 HuSH STAT5A Constructs against signal transducer and activator of transcription 5A 380
TR200329 6777 HuSH STAT5B Constructs against signal transducer and activator of transcription 5B 380
TR200330 6794 HuSH STK11 Constructs against serine/threonine kinase 11 (Peutz-Jeghers syndrome) 380
TR200331 7026 HuSH NR2F2 Constructs against nuclear receptor subfamily 2, group F, member 2 380
TR200332 7046 HuSH TGFBR1 Constructs against transforming growth factor, beta receptor I (activin A receptor type II-like 380
kinase, 53kDa)
TR200333 7075 HuSH TIE Constructs against tyrosine kinase with immunoglobulin and epidermal growth factor homology domains 380
TR200334 7101 HuSH NR2E1 Constructs against nuclear receptor subfamily 2, group E, member 1 380
TR200335 7128 HuSH TNFAIP3 Constructs against tumor necrosis factor, alpha-induced protein 3 380
TR200336 7301 HuSH TYRO3 Constructs against TYRO3 protein tyrosine kinase 380
TR200337 7421 HuSH VDR Constructs against vitamin D (1,25- dihydroxyvitamin D3) receptor 380
TR200338 7465 HuSH WEE1 Constructs against WEE1 homolog (S. pombe) 380
TR200339 7535 HuSH ZAP70 Constructs against zeta-chain (TCR) associated protein kinase 70kDa 380
TR200340 7918 HuSH BAT4 Constructs against HLA-B associated transcript 4 380
TR200341 8536 HuSH CAMK1 Constructs against calcium/calmodulin-dependent protein kinase I 380
TR200342 8711 HuSH TNK1 Constructs against tyrosine kinase, non-receptor, 1 380
TR200343 8717 HuSH TRADD Constructs against TNFRSF1A-associated via death domain 380
TR200344 8772 HuSH FADD Constructs against Fas (TNFRSF6)-associated via death domain 380
TR200345 8792 HuSH TNFRSF11A Constructs against tumor necrosis factor receptor superfamily, member 11a, activator of NFKB 380
TR200346 8986 HuSH RPS6KA4 Constructs against ribosomal protein S6 kinase, 90kDa, polypeptide 4 380
TR200347 9088 HuSH PKMYT1 Constructs against membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase 380
TR200348 9113 HuSH LATS1 Constructs against LATS, large tumor suppressor, homolog 1 (Drosophila) 380
TR200349 9261 HuSH MAPKAPK2 Constructs against mitogen-activated protein kinase-activated protein kinase 2 380
TR200350 9328 HuSH GTF3C5 Constructs against general transcription factor IIIC, polypeptide 5, 63kDa 380
TR200351 9344 HuSH TAOK2 Constructs against TAO kinase 2 380
TR200352 9578 HuSH CDC42BPB Constructs against CDC42 binding protein kinase beta (DMPK-like) 380
TR200353 10155 HuSH TRIM28 Constructs against tripartite motif-containing 28 380
TR200354 10188 HuSH ACK1 Constructs against activated Cdc42-associated kinase 1 380
TR200355 10221 HuSH TRIB1 Constructs against tribbles homolog 1 (Drosophila) 380
TR200356 10392 HuSH CARD4 Constructs against caspase recruitment domain family, member 4 380
TR200357 10783 HuSH NEK6 Constructs against NIMA (never in mitosis gene a)-related kinase 6 380
TR200358 22983 HuSH MAST1 Constructs against microtubule associated serine/threonine kinase 1 380
TR200359 23043 HuSH TNIK Constructs against TRAF2 and NCK interacting kinase 380
TR200360 23235 HuSH SIK2 Constructs against salt-inducible serine/threonine kinase 2 380
TR200361 23604 HuSH DAPK2 Constructs against death-associated protein kinase 2 380
58 www.origene.com
Product offering is subject to change. Refer to www.origene.com for the most updated information.
Hu
SH
™Pr
emad
esh
RNA
expr
essi
onpa
nels
Cat# GeneID/LocusID description price
TR200362 26524 HuSH LATS2 Constructs against LATS, large tumor suppressor, homolog 2 (Drosophila) 380
TR200363 27102 HuSH HRI Constructs against heme-regulated initiation factor 2-alpha kinase 380
TR200364 51755 HuSH CRK7 Constructs against CDC2-related protein kinase 380
TR200365 51776 HuSH ZAK Constructs against sterile alpha motif and leucine zipper containing kinase AZK 380
TR200366 55072 HuSH RNF31 Constructs against ring finger protein 31 380
TR200367 55589 HuSH BMP2K Constructs against BMP2 inducible kinase 380
TR200368 55739 HuSH FLJ10769 Constructs against hypothetical protein FLJ10769 380
TR200369 65018 HuSH PINK1 Constructs against PTEN induced putative kinase 1 380
TR200370 79012 HuSH MGC8407 Constructs against hypothetical protein MGC8407 380
TR200371 85443 HuSH KIAA1765 Constructs against KIAA1765 protein 380
TR200372 112858 HuSH TP53RK Constructs against TP53 regulating kinase 380
TR200373 114783 HuSH LMTK3 Constructs against lemur tyrosine kinase 3 380
TR200374 9284 HuSH NPIP Constructs against NM_006985 - Locus_ID 9284 380
59ph: 1.888.267.4436 fax: 1.301.340.9254
Product offering is subject to change. Refer to www.origene.com for the most updated information.
Hu
SH
™pR
Sve
ctor
60 www.origene.com
Product Listing Cat. No. PricepRS retroviral gene silencing vector TR20003 150
pRS-GFP-v1 (shRNA) plasmid TR20001 150
pRS-Luc-v1 (shRNA) plasmid TR20002 150
pRS vector
Introduction:
Other than the premade shRNA plasmids, OriGene
also provides the blank pRS vector for researchers to
clone in their own shDNA sequences.
The vector system has been validated for retrovirus
production, puromycin selection, stable cell line
production, shRNA expression and gene silencing.
Oligos against target genes are designed and the
oligo pairs are annealed and inserted into the BamHI
– BbsI sites of the pRS plasmid. Due to significant
error rates associated with oligo synthesis, sequence
verification of the shRNA insert is highly re-
commended. OriGene only sells sequence verified
shRNA plasmids.
Due to the biological complexity of the RNAi, only
about 25-30% of the shRNA vectors are expected to
reduce the target gene expression by >70%. The first
step using shRNA vectors is to validate the
functionality of the vectors in transiently transfected
cells. We recommend to co-transfect cells, i.e.,
HEK293, with the shRNA vector and the target gene
to determine the effect of the shRNA vectors against
the target gene and to select only the ones with
inhibitory function.
Once a specific shRNA vector(s) is selected, stable cell
lines can be established by transfecting the plasmid
into a retroviral packaging cell and by using the virus
containing supernatant to infect the target cells.
Puromycin is added to the infected cells for selecting
stable cell lines expressing the functional shRNA.
Applications
• Cloning of shRNA oligos
• Inhibiting the expression of the target genes
• Generating stable cell lines
shRNA oligos
pBS Ori
pRS5522 bp
U6 promoter
Ampicillin
SV40 early promoter
Puro
3‘ LTR5‘ LTR
BamH I
Not I
Hind IIIBbs I
Sal I
Cla I
Eco R I
Fig. 1
pRS plasmid map. The shRNA oligos will be cloned between
BamHI and BbsI, immediately downstream of U6 promoter. A
puromycin selection marker is present. Both 5’ and 3’ LTRs are
from MMLV and used to generate retroviruses in appropriate
packaging cells.
Hu
SH
™pR
Sve
ctor
61ph: 1.888.267.4436 fax: 1.301.340.9254
pRS-GFP-v1 (shRNA) plasmid
Product Description
Enhanced green fluorescent protein (EGFP) shRNA
expression plasmid. Transfection grade purified
mammalian expression plasmid carrying GFP se-
quence. When transfected into mammalian cells, it
expresses a short-haripin RNA (shRNA) from the U6
promoter, resulting in the inhibition of the target
gene. Significant inhibition of the GFP expression
can be detected using both fluorescence microscopy
and western blot analysis with GFP specific antibody
(Fig. 2).
Applications: Positive control for the inhibition of
EGFP expression
Product content: 10 ug of pRS-luc-v1 and 10 ug of
pRS-NegCon-v1
Fig. 2
shRNA plasmid pRS-GFP-v1 plasmid. HEK293 cells were
transfected with plasmids carrying eGFP (A), eGFP and shRNA-GFP-
v1 at 1:100 ratio (B), eGFP and NegCont-v1 (C) using FuGene 6.
Three days after transfection, the cells were analyzed on a
fluorescence microscope and on Western blot with a GFP specific
antibody.
pRS-Luc-v1 (shRNA) plasmid
Product Description
Firefly luciferase (Luc) shRNA expression plasmid.
Transfection grade purified mammalian expression
plasmid carrying luciferase sequence. When
transfected into mammalian cells, it expresses a
short-haripin RNA (shRNA) from the U6 promoter,
resulting in the inhibition of the target gene. In HEK
cells co-transfected with a luciferase plasmid and
pRS-luc-v1, approximately 90% inhibition of the
luciferase activity can be observed (Fig. 3).
Applications: Positive control for the inhibition of
luciferase expression
Product content: 10 ug of pRS-luc-v1 and 10 ug of
pRS-NegCon-v1
shRNA plasmid pRS-Luc-v1 plasmid. HEK293 cells were
transfected with plasmids carrying Luciferase (Pos. Cont.), Luc and
shRNA-Luc-v1 at 1:100 ratio, eGFP and NegCont-v1 using FuGene 6.
Three days after transfection, the cells were analyzed with Britelite
(PE) on Victor 3 plate reader (PE).
Fig. 3
62 www.origene.com
Full-Length cDNA cloningRapid-Screen™ arrayed cDNA libraries
Rapid-Screen™ Longest-CloneSM services
Sure-RACE™ 5’-RACE Kit
Yeast two-hybrid expression cDNA libraries
Full-
len
gth
cDN
Acl
on
ing
Rapi
dSc
reen
™ar
raye
dcD
NA
libra
ries
64 www.origene.com
Product Listing Cat. No. PriceHuman (Master plates)
Human Brain (adult) LAB-1001 925
Human Brain (fetal) LLFB-1001 925
Human Colon LCO-1001 925
Human Heart LHT-1001 925
Human Kidney LKD-1001 925
Human Liver LLI-1001 925
Human Lung LLU-1001 925
Human Peripheral blood leukocytes LPBL-1001 925
Human Placenta LLPC-1001 925
Human Skeletal muscle LMU-1001 925
Human Small intestine LSI-1001 925
Human Spleen LLSP-1001 925
Human Testis LTS-1001 925
Sub-Plates
All sub-plates for the above libraries [Sub-plates are designated by the library’s 100prefix followed by the number (column) and letter (row) of the positive well(s) detected on the master plate, e.g. “RAB-5A”]
Rapid Screen™ arrayed cDNA libraries
The complete thirteen human and/or six mouse cDNA library panels are available for licensing to investigators
who are interested in simultaneous multi-tissue screens to:
1) increase the chances of identifying “full-length” cDNA clones that are present in more than one tissue, or
2) isolate cDNA clones of genes whose major sites of expression have not been fully described.
Full-
len
gth
cDN
Acl
on
ing
Rapi
dSc
reen
™ar
raye
dcD
NA
libra
ries
65ph: 1.888.267.4436 fax: 1.301.340.9254
Product Listing Cat. No. PriceMouse (Master plates)
Mouse Brain (adult) MAB-1001 925
Mouse Embryo (12.5-day) MEB-1001 925
Mouse Embryo (19-day) MEA-1001 925
Mouse Liver MLI-1001 925
Mouse Testis MTS-1001 925
Mouse Thymus MTM-1001 925
Rat (Master plates)
Rat Brain (adult) RAB-1001 925
Sub-Plates
All sub-plates for the above libraries [Sub-plates are designated by the library’s 100prefix followed by the number (column) and letter (row) of the positive well(s) detected on the master plate, e.g. “RAB-5A”]
Introduction:
Gene identification is increasing at a rapid pace, due
in large part to technologies such as expressed
sequence tag (EST) discovery, as well as the wealth
of sequence information being produced by the
Genome Sequencing Projects. Once a gene is
identified, the next step is to clone the full-length
cDNA in order to perform sequence and expression
analyses, and ultimately to discover its function in
vivo.
Although it has been suggested that most of the
human genes have already been tagged, full-length
cDNA clones have been obtained for less than 50
percent of them. There are several reasons for this
lag. Until now, obtaining a full-length clone has been
very labor-intensive, time consuming and expensive.
More importantly, the technology for constructing
full-length libraries from which clones are isolated
has lagged behind the technology for tagging the
genes in the first place. For example, most libraries
to date have a relatively low average insert size, due
to the fact that it has been difficult to reverse
transcribe mRNAs with extensive secondary
structures and that smaller-sized cDNAs are much
more easily ligated into a vector than larger cDNAs.
The Rapid-Screen™ Arrayed cDNA Library Panels*
were designed as a fast, inexpensive, and easy way to
clone full-length cDNAs. The library quality was
improved by first isolating double-stranded cDNAs of
different size-fractions and then ligating them se-
parately into the vector. The cloning process has been
made faster and more economical by having the
libraries arrayed in 96-well plates and developing a
screening procedure that is PCR based. Since the
screening is PCR-based, vector and gene-specific
primers can be used in concert to determine the size of
the various cDNA inserts after the very first PCR screen,
Full-
len
gth
cDN
Acl
on
ing
Rapi
dSc
reen
™ar
raye
dcD
NA
libra
ries
66 www.origene.com
thus eliminating the need to isolate many clones and
then find out which, if any, are full-length. A screening
service is also offered, in which OriGene will simul-
taneously screen all twelve human or all six mouse
Rapid-Screen™ cDNA libraries and provide the longest
clone of any gene of interest found in the libraries.
*U.S. Patent #6,316,193
Product Description:
How does Rapid-Screen™ work?
The Rapid-Screen™ cDNA Library Panels were
designed for EST to “full-length” gene cloning and
require just three sets of PCRs to identify the desired
cDNA clone. The first PCR analysis is performed in a
96-well “Master Plate,” where each well contains
plasmid DNA from 5,000 clones. Having identified the
positive well(s) by gel electrophoresis of the reaction
products, the second PCR analysis is carried out in the
corresponding 96-well “Sub-Plate(s),” which contains
all 5,000 clones from the positive well of the Master
Plate arrayed at only 50 clones per well. Since this
plate contains glycerol stocks of E. coli instead of
plasmid DNA, the cells can be diluted and plated out
once a positive well(s) has been identified. Analysis of
96 single colonies by PCR leads to the identification of
the positive clone from the Sub-Plate well.
To ensure that a clone detected in a specific well in the
Master Plate will be represented in the appropriate
Sub-Plate, the Sub-Plates were not derived by dilution
of individual wells from the Master Plate (a process
which may lose specific clones). Instead, the Sub-
Plates were generated first and the wells from each
Sub-Plate were then pooled to generate the Master
Plate. To guarantee that clones from different wells in
the Master Plate were each independently-derived, the
Sub-Plates were generated with primary trans-
formants that had not been amplified.
ADVANTAGES
Isolate large cDNA clones with only three 96-well PCRs
Libraries designed tocontain large inserts
Can identify longest cloneeven before its isolation
Final clone is not aPCR product
No messy radioactive probes or labor-intensive
colony lifts
“Full-length” clones canbe immediately transfected
into mammalian cells
Full-
len
gth
cDN
Acl
on
ing
Rapi
dSc
reen
™ar
raye
dcD
NA
libra
ries
67ph: 1.888.267.4436 fax: 1.301.340.9254
What was done to obtain large inserts?
A great deal of foresight has been incorporated into
the development of these arrayed cDNA panels, so
that they are user-friendly and cost-effective. Since
they are intended for researchers searching for
longer than average-sized cDNA clones, double-
stranded cDNAs (synthesized from poly A+ RNA
using oligo (dT) primers) were size-fractionated in a
low-melt agarose gel and the various size-fractions
were independently ligated to the vector to minimize
competition for insertion by smaller cDNAs. The
insert sizes of random clones from the various
ligation reactions were then determined (Fig. 1). This
allowed the Rapid-Screen™ libraries to be spiked
with more clones from the large-sized fraction,
thereby giving them a much larger average insert
size (Table 1). In addition to helping find full-length
clones, a large average insert size allows for
Fig. 1
14
12
10
8
6
4
2
0
Num
ber
ofco
loni
es
1.5 - 2.5 kb fraction2.5 - 5 kb fraction> 5kb fraction
0.5 1 1.5 2 2.5 3 3.5 4 4.5 5 6kb
*Data for this table were obtained by randomly picking 48 colonies per library, preparing plasmid DNA from each, and analyzing the insertsby restriction enzyme digestion followed by gel electrophoresis.
Brain (adult)Brain (fetal)ColonHeartKidneyLiverLungPeripheral blood leukocytesPlacentaSkeletal muscleSmall intestineSpleenTestis
96989394889591829284919882
0.9-6.00.9-10.00.8-6.51.0-9.00.8-3.70.8-3.21.0-6.01.0-5.01.0-9.00.4-6.50.5-6.00.5-10.00.6-5.5
2.42.82.92.91.82.03.22.43.62.52.62.42.5
Brain (adult)Embryo (12.5-day)Embryo (19-day)LiverTestisThymus
919499949789
0.6-10.02.0-7.00.5-6.51.0-6.51.2-8.00.6-8.0
3.23.53.53.53.53.2
Human
Mouse
Brain (adult) 900.9-5.02.6Rat
LIBRARY % INSERTSSIZE RANGEAVERAGE SIZE (kb)
Table 1*
Full-
len
gth
cDN
Acl
on
ing
Rapi
dSc
reen
™ar
raye
dcD
NA
libra
ries
68 www.origene.com
screening of fewer members due to the general
finding that longer transcripts tend to be less
abundant in the cell. Since the cDNAs were
directionally-cloned into the CMV expression vector
pCMV6-XL4 (Fig. 2), a vector-derived 5’ PCR primer
can be used in conjunction with a gene-specific 3’
reverse primer to identify the longest cDNA clone of
interest (see Fig. 4).
Can the clones be expressed inmammalian cells?
Once a full-length clone has been isolated and
sequenced, it can be directly transfected into
mammalian cells for expression studies. The library
plasmid, pCMV6-XL4, contains the SV40 origin of
replication for propagation in mammalian cells.
Moreover, the plasmid contains the CMV promoter,
which will drive expression of the insert if it contains
a translational start site. Finally, the human growth
hormone polyadenylation signal is present to effect
proper transcriptional termination.
PRODUCT COMPONENTS
One 96-well Master Plate(enough material for
5 screens)
5’-vector primer
Four sheets of aluminum foil sealing tape Fig. 2
ColElOri
SV40 Ori
f1 Ori
Ampicillin
hGH poly A
Sac I
T7 promoterM13 reverse
Sma I
MCS
CMVpromoter
pCMV6-XL4
Full-
len
gth
cDN
Acl
on
ing
Rapi
dSc
reen
™ar
raye
dcD
NA
libra
ries
69ph: 1.888.267.4436 fax: 1.301.340.9254
1 Perform 96-well PCR on the Master
Plate (5,000 clones/well) using 2 gene-
specific primers; run samples in an
agarose gel to identify positive well(s);
order corresponding Sub-Plate.
2 Perform 96-well PCR
on the Sub-Plate (50 clones/well)
using same primers as for Master
Plate; run samples in an agarose gel
to identify positive well(s); dilute and
plate positive well on an LB amp plate
to obtain single colonies.
3 Perform PCRs on 96 individual colonies
using same primers as above; run
samples in an agarose gel to identify
positive colony; grow cells and prepare
miniprep DNA; analyze plasmid DNA
by restriction enzyme digestion and
DNA sequencing.
Product Use
Full-
len
gth
cDN
Acl
on
ing
Rapi
dSc
reen
™ar
raye
dcD
NA
libra
ries
70 www.origene.com
Product Application
Is a 96-well PCR necessary?
If it is not possible or desirable to perform 96
simultaneous PCRs, then pools of DNA from the
wells in the Master Plate can be made. Using 5 µL of
DNA from each well of the Master Plate, twelve pools
(40,000 clones/pool) of DNA can be made from
columns 1 through 12, and/or eight pools (60,000
clones/pool) of DNA can be made from rows A
through H. These 20 pools can be analyzed by PCR
using two gene-specific primers. In the example
shown in Fig. 3, there is a single clone in this library
and it is located in well E9 of the Master Plate. The
one caveat to this approach is that since there are
40,000-60,000 independent clones per pool, rather
than only 5,000 from an individual well, both gene-
specific primers must be highly specific. The pooling
technique is most informative when working with a
low-abundance versus a high-abundance transcript.
Can the size of the longest clone bedetermined without first isolating it?
If multiple positive clones are detected, one may
wish to determine their insert sizes before
proceeding to their isolation. In the example shown
in Fig. 4, PCR analysis using a gene-specific primer-
pair (panel A) revealed positive clones in pools 1, 4
and 9 (40,000 clones/pool). Since the inserts were
directionally cloned into the library vector,
simultaneous analysis using the 5’ vector primer and
the 3’ gene-specific primer (panel B) allowed
determination of the extent of upstream sequences
(5.5 kb in two of the clones and 3.5 kb in the other).
Similarly, use of the 5’ gene-specific primer with the
3’ vector primer (panel C) allowed determination of
the extent of downstream sequences (1.5 kb in all
three clones). In this example, one could determine
the insert sizes to be 6.5 kb in two clones and 4.5 kb
in the other, even before proceeding to their
isolation. The 6.5-kb clones turned out to be full-
length.
ABCDEFGH
1 2 3 4 5 6 7 8 9 10 11 12
Fig. 3
A B C D E F G H
1 2 3 4 5 6 7 8 9 10 11 12
A
kb
— 0.5
B
Pool
1
Pool
4
Pool
9
C
— 5.5— 3.5
— 1.5
1 2 3 4 5 6 7 8 9 10 11 12
Fig. 4
Full-
len
gth
cDN
Acl
on
ing
Rapi
dSc
reen
™ar
raye
dcD
NA
libra
ries
71ph: 1.888.267.4436 fax: 1.301.340.9254
What factors are important when “long” PCR is used with Rapid-Screen™?
When the 5’ vector primer is used in conjunction with a
3’ gene-specific primer to identify long clones, the
choice of the appropriate Taq DNA polymerase is
important. Fig. 5 illustrates the use of three different
enzymes (labeled 1, 2 and 3), with either a 3-min (top) or
a 7-min (bottom) extension time, on four selected
Master Plate wells (5,000 clones/well), each containing
a single positive clone with insert sizes ranging from 2.5
to 8.0 kb in length. Enzyme 1 was able to amplify all of
the fragments using only a 3-min extension time, so the
7-min extension time experiment was not necessary.
Enzyme 2 was not able to amplify any of the fragments
with a 3-min extension time, but it could successfully
amplify the 2.5- and 3.0-kb fragments when a 7-min
extension time was used. Enzyme 3 partially amplified
two of the fragments when a 3-min extension time was
used, but it could successfully amplify all four
fragments with a 7-min extension time. These
experiments demonstrate the importance of choosing
an efficient Taq DNA polymerase. If only small
fragments are being amplified, then any of the three
enzymes tested are suitable. To amplify longer
fragments, a more efficient Taq DNA polymerase and/or
a longer extension time is required.
Can Rapid-Screen™ be used to clone different forms of an alternatively-spliced transcript?
Rapid-Screen™ panels can be used to clone
alternatively-spliced transcripts, even if the desired
transcript is less abundant than its parent transcript.
The detection of different-sized fragments by using
the 5’ vector primer plus the 3’ gene-specific primer
is sometimes attributable to alternative splicing
rather than to truncated transcripts (if the alternative
splicing has already been characterized, then gene-
specific primers that differentiate between the two
spliced forms can be designed). As shown in Fig. 6, a
pair of gene-specific primers was used on a Master
Plate now to detect two alternatively-spliced
transcripts of a particular gene. In this instance, the
longer transcript was more abundant than the
shorter form; the former appeared in three wells
(5,000 clones/pool) and the shorter form in two wells.
MM M
Enz 1 Enz 2 Enz 3
Fig. 6
Fig. 5
1 2 3 4 5 6 7 8 9 10 11 12
Full-
len
gth
cDN
Acl
on
ing
Rapi
d-Sc
reen
™Lo
nges
t-Clo
neSM
serv
ices
72 www.origene.com
Product Description
What is the Screening Service?
OriGene offers customized screening of either a 12-
tissue human or a 6-tissue mouse Rapid-Screen™
Arrayed cDNA Library Panel* (Super-Plate), and
subsequent isolation of the “longest clone” from the
selected library. Since most genes are expressed in
several tissues, the screening of a multi-tissue panel,
containing a total of either 6-million human or 3-
million mouse size-selected clones, increases the
probability of isolating a full-length clone. For
example, even though a particular transcript may be
known to be abundant in a certain tissue, differences
in starting RNA quality or in cDNA yield for
individual libraries may result in distortions in
representation. In other words, the library with the
most representations of a particular gene does not
assure the presence of a full-length clone.
How is the screen performed?
Initially, PCR will be performed using gene-specific
primers, either provided by the customer or
designed by OriGene, to determine whether clones
can be identified in any of the libraries. If no clones
are found, the customer will not be charged and the
service will be terminated. However, if the initial
screening is successful, then the relative sizes of the
cDNA clones will be determined and the longest
clone from the libraries will be isolated. Hence, there
is no need to provide multiple clones. Restriction
enzyme and PCR analyses will be performed on
purified DNA prepared from the clone to confirm
both its integrity and the size of the cDNA insert. An
aliquot of the purified DNA, a bacterial stock of the
clone, and a photograph of an ethidium bromide-
stained agarose gel detailing the analyses will be
provided to the customer.
What are the twelve human librariescurrently available?
Brain (adult), brain (fetal), heart, kidney, liver, lung,
muscle, peripheral blood leukocytes (PBL), placenta,
small intestine, spleen, and testis.
What are the six mouse libraries currently available?
Brain (adult), embryo (12.5-day), embryo (19-day),
liver, testis, and thymus.
*U.S. Patent #6,316,193
Product Listing Cat. No. Price
Human Library Screening Service HRSS-001 3900
Mouse Library Screening Service MRSS-001 3900
Primer Design and Synthesis RSPD-001 300
Rapid-Screen™ Longest-CloneSM services
Full-
len
gth
cDN
Acl
on
ing
Rapi
d-Sc
reen
™Lo
nges
t-Clo
neSM
serv
ices
73ph: 1.888.267.4436 fax: 1.301.340.9254
What materials need to be provided forthis service?
1. Set of exonic PCR primers. One forward (5’) and
two contiguous reverse (3’) gene specific primers are
required. The distance between the positions of the
forward and reverse primers within the cDNA should
ideally be about 500 base-pairs. This information is
required prior to the screening. OriGene can design
and synthesize specific primers for an additional
charge.
2. Positive control DNA. A cDNA clone, upon which
the PCR primer sequences were based, is required
and will be used as a positive control.
3. Information on the target gene (optional). This
includes (i) expected size of target mRNA based on
Northern blotting, (ii) expected sites of expression of
target mRNA, and (iii) sequence of EST or
incomplete cDNA used to generate the PCR primers
(only if deemed appropriate).
How is the initial screen performed?
The first step in the Rapid-Screen™ Longest-CloneSM
Screening Service involves a Super-Plate screen, in
which each of the eight rows from all twelve of the
human or all six of the mouse panels have been
combined into pools (60,000 clones/pool, eight
pools/library). If no clones are detected in the Super-
Plate screen, there is no charge for the screen.
However, if positives are detected, which is usually
the case, then the well containing the longest clone
will be determined using the 5’ vector-specific primer
in combination with the 3’ gene-specific primer (see
Fig. 4 of the Rapid-Screen™ Arrayed cDNA Library
Panels section). The longest clone will then be
isolated from the library in which it is found. Fig. 1
shows the results of a typical human Super-Plate
screen. In this example, the cDNA of interest was
absent in fetal brain, liver, and spleen. It was
detected in low abundance in adult brain, heart,
kidney, muscle, PBL, and testis. The cDNA was found
in high abundance in lung, placenta, and small
intestine.
Brain (adult)
Brain (fetal)
Heart
Kidney
Liver
Lung
Muscle
PBL
1 2 3 4 5 6 7 8 1 2 3 4
Placenta
Small
Intestine
Spleen
Testis
Fig. 1
1 2 3 41 2 3 4 5 6 7 8
Full-
len
gth
cDN
Acl
on
ing
Rapi
d-Sc
reen
™Lo
nges
t-Clo
neSM
serv
ices
74 www.origene.com
What characterizations are performed onthe isolated clone?
Once the longest cDNA clone has been isolated, its
identity will be confirmed by PCR analysis. The size
of the cDNA insert will be determined by restriction
enzyme mapping and by PCR, using vector-specific
primers and purified plasmid DNA. Fig. 2 shows such
an analysis by agarose gel electrophoresis for an 8-kb
clone that was obtained by the Screening Service.
Purified plasmid DNA of the clone was digested by
Not I, which cuts on either side of the insert (lane 1).
The upper band is the insert while the lower band is
the vector. PCR was performed using two gene-
specific primers to confirm the identity of the clone
(lane 2). The length of the 3’ end of the clone was
determined by PCR using a 5’ gene-specific primer
and a 3’ vector-specific primer (lane 3). The length of
the 5’ end of the clone was determined by PCR using
a 5’ vector-specific primer and a 3’ gene-specific
primer (lane 4).
Further Information
Before ordering, it is recommended that customers
first contact OriGene for further details about the
Rapid-Screen™ Longest-CloneSM Screening Service.
Please call toll-free at 888-267-4436 (U.S. only) or at
301-340-3188, or contact us by e-mail at
custsupport@origene.com.
Fig. 2
— 2
— 1
— 8
kb
— 4
M 1 2 3 4 M
— 0.4
Full-
len
gth
cDN
Acl
on
ing
Sure
-RAC
E™5’
-RAC
Eki
t
75ph: 1.888.267.4436 fax: 1.301.340.9254
Introduction
Traditional cDNA libraries frequently yield clones
which are incomplete and where sequences
representing the mRNA 5’ ends are missing. The
RACE technology, a PCR-based approach for rapid
amplification of cDNA ends, has been particularly
useful for completing the missing portions of cDNA
clones and yielding full-length sequences of
expressed regions of genes.
To expand the utilities of the RACE technology,
OriGene has now developed a PCR-ready RACE
panel which will allow simultaneous 5’ end analysis
of transcripts from 24 individual human tissues or 24
mouse tissues and developmental stages. The Sure-
RACE™ panels contain double-stranded cDNAs that
have been arrayed in multi-well plates. Sure-RACE™
is designed with the recognition that an increasing
number of genes have been identified that utilize
alternate RNA start-sites resulting from the use of
tissue-specific or developmental stage-specific
transcriptional promoters. As well, an increasing
number of genes have also been found to utilize
alternate 5’ exons, sometimes resulting in protein
products with different N-terminal amino acid
sequences.
Product Listing Cat. No. PriceHuman Sure-RACE™ Panel HRAA-101 620(4 complete panels)
Mouse Sure-RACE™ Panel MRAB-101 495(4 complete panels)
Sure-RACE™ 5’-RACE kit
ADVANTAGES
Broad spectrum of tissues:scan 24 individual human or
mouse tissues
High sensitivity: detect rare transcripts
Simultaneous analysis ofalternatively-spliced
transcripts and alternateRNA start-sites
Full-
len
gth
cDN
Acl
on
ing
Sure
-RAC
E™5’
-RAC
Eki
t
76 www.origene.com
Product Description
Development of the Sure-RACE™ Panels began with
the selection of high-quality mRNAs. The integrity of
each of the poly A+ RNA was determined by Northern
blot analysis using ß-actin cDNA as the hybridization
probe. Only mRNAs that fulfilled stringent criteria of
intactness were used for cDNA synthesis.
It is well-established that mRNAs have complex
secondary structures which may act as strong stops
during first-strand cDNA synthesis using standard
reverse transcriptase (RT). As a consequence, the
resulting single-stranded cDNAs may not be
representative of the starting mRNAs in that their 5’
ends may not be reverse transcribed. OriGene has
successfully used a dual-cycling procedure to
overcome this problem. Specifically, the initial cDNA
synthesis was performed using standard procedures
with MMLV RT at 42°C, followed by a second round of
synthesis with thermostable Tth DNA polymerase at
75°C. Cycling of high-processivity/low-thermostability
enzyme and low-processivity/high-thermostability
enzyme, which facilitates read-through of RNA
secondary structures, was repeated a second time to
ensure the efficiency of the reaction.
Conventional double-stranded cDNA synthesis
involves the “blunting” of the cDNAs, which results
in the loss of 5’ end sequences of 20-200 nucleotides.
To avoid sequence loss, the first-strand cDNAs were
“tailed” at their 3’ ends with dCTP (see Fig. 1). The
terminal transferase reaction was optimized to
produce a tail of 12-15 dC residues. Second-strand
cDNA synthesis was initiated by the annealing of an
adaptor-oligo(dG) primer to the oligo(dC) tail at the 3’
ends of the first-strand cDNA molecules. Escherichia
coli DNA polymerase I was used for second-strand
synthesis to reduce the nucleotide-incorporation
error rate. Additionally, RNase H was added to nick
the mRNA and to allow internal priming. Finally, T4
DNA ligase was added to facilitate joining of
fragments primed by oligo(dG) and nicked mRNA.
The Sure-RACE™ panels were carefully assembled
from 24 major human tissues or 24 major mouse
tissues and different developmental stages. The
RACE-ready cDNAs are provided in two
3’CCCCCCCCCCC
5’ADP1..ADP2..GGGGGGGGGGG
5’
5’
3’
3’CCCCCCCCCCC
3’CCCCCCCCCCC
5’ADP1..ADP2..GGGGGGGGGGGH
Dual cycling synthesis
dCTP tailing
Second strand synthesis
fig.SR-1
VTTTTTTTTTTTTTTCTCGAG 5’
AAAAAAAAAAAAAA 3’
AAAAAAAAAAAAAA 3’
TTTTTTTTTTTTTTCTCGAG 5’
TTTTTTTTTTTTTTCTCGAG 5’
AAAAAAAAAAAAAA 3’
AAAAAAAAAAAAAAGAGCTC 3’
TTTTTTTTTTTTTTCTCGAG 5’
AAAAAAAAAAAAAA 3’
Fig. 1
Full-
len
gth
cDN
Acl
on
ing
Sure
-RAC
E™5’
-RAC
Eki
t
77ph: 1.888.267.4436 fax: 1.301.340.9254
concentrations (5x and 1x), to accommodate the
analysis of both rare and abundant transcripts. They
are arrayed in 48-well (6 x 8 wells) PCR plates. Four
panels are provided per order to allow replication of
the findings and assurance of the specificity of the
observed RACE products.
Product Use
By simply performing a nested PCR analysis, using
the two contiguous adaptor-specific primers
provided with the kit and two contiguous gene-
specific primers designed by the investigator, the
Sure-RACE™ panels will: [1] markedly increase the
probability of completing the 5’ ends of transcripts
which are rare or have complex secondary
structures; [2] allow the identification of alternate
RNA start-sites either within the same tissue, in
different tissues or at different developmental
stages; and [3] provide evidence for alternate RNA
splicings at the 5’ end, such as the use of alternate
first exons or an identical first exon but alternate
downstream exons, which may result in proteins
with nonidentical amino acid sequences. Any or all of
this information is derived from direct sequencing of
the PCR products generated by the human or mouse
Sure-RACE™ panels.
Sure-RACE™ is performed using two rounds of PCR.
The first round uses Adaptor Primer 1 and a gene-
specific primer to enrich for the specific cDNAs, and
the second round uses Adaptor Primer 2 and a
nested gene-specific primer to further amplify the
specific target(s). About 20 PCR cycles are routinely
performed in the first round, followed by 30-35
cycles in the second round (nested) PCR. The
specificity of the gene specific primers is a critical
factor.
PRODUCT COMPONENTS
Four identical 48-well PCRplates containing two
dilutions of 24 human or24 mouse RACE-ready cDNAs
Adaptor primers for PCR
Control primers fortransferrin receptor
Free sample of DNAQuanti-Ladder™
Full-
len
gth
cDN
Acl
on
ing
Sure
-RAC
E™5’
-RAC
Eki
t
78 www.origene.com
5 Document the results by imaging/
photography and elute bands of interest
for DNA sequence analysis or cloning.
1 Prepare a 48-well PCR master mix including
Adaptor Primer 1 and gene-specific primer 1;
aliquot the solution into each well of the
Sure-RACE™ panel.
2 Place the plate into a
thermal cycler and
proceed with
primary PCR.
3 Dilute primary PCR and
perform secondary PCR in
second 48-well plate using
Adaptor Primer 2 and
gene-specific primer 2.
4 Upon completion of the secondary PCR,
analyze the reaction products by agarose
gel electrophoresis.
Product Use
Full-
len
gth
cDN
Acl
on
ing
Sure
-RAC
E™5’
-RAC
Eki
t
79ph: 1.888.267.4436 fax: 1.301.340.9254
Product Application
A. Human Sure-RACE™ Panel
The Human Sure-RACE™ panel was assembled from
24 major tissues. The RACE-ready cDNAs are
provided in two concentrations (5x and 1x) to
accommodate the analysis of both rare and
abundant transcripts. They are arrayed in a 48-well (6
x 8 wells) PCR plate in the order indicated in Fig. 2.
The Human Sure-RACE™ panel was used to analyze
the 5’ end sequences of transcripts from the highly-
expressed transferrin receptor gene (Fig. 3). The
results show a single major component of the
appropriate size in virtually every tissue, as well as
minor components of heterogeneous sizes which
were derived from cDNAs with truncated 5’ ends.
When the template concentration was reduced to
one-fifth, the latter components became less
detectable.
The Sure-RACE™ panel can be used to complete the
5’-end sequences of long and rare transcripts with
extensive secondary structure (Fig. 4). The ataxia
telangiectasia (ATM) gene encodes an ubiquitous 12-
kb transcript with extensive conformations and
somewhat heterogeneous 5’ ends.
B. Mouse Sure-RACE™ Panel
The Mouse Sure-RACE™ panel was assembled from
24 major tissues and developmental stages. The
adult tissues were prepared from outbred Swiss
Webster mice and the breast tissues from outbred
CD1 mice. The RACE-ready cDNAs are provided in
two concentrations (5x and 1x) to accommodate the
analysis of both rare and abundant transcripts. They
are arrayed in a 48-well (6 x 8 wells) PCR plate in the
order indicated in Fig. 5.
Examples of applications of the Mouse Sure-RACE™
panel are illustrated here. The Sure-RACE™ panel
can be used to analyze the 5’ end sequences of
transcripts. Figure 6 shows an analysis if the 5’ end
of the interleukin 1 receptor antagonist mRNA. The
2
2
8
8
4
4
A
B
C
D
E
F
G
H
1 2 3 4 5 6 7 8 9 10 11 12
Pituitary GlandThyroid GlandAdrenal GlandPancreasOvaryUterus
Fig. SR-7
1
1
2
2
3
3
4
4
5
5
6
6
7
7
8
8
9
9
10 11 12
13 14 15 16 17 18
19 20 22 23 24
10 11 12
13 14 15 16 17 18
21
19 20 22 23 2421
1
1
2
2
3
3
4
4
5
5
6
6
7
7
8
8
9
9
10 11 12
13 14 15 16 17 18
19 20 22 23 24
10 11 12
13 14 15 16 17 18
21
19 20 22 23 2421
1.2.3.4.5.6.
13.14.15.16.17.18.
7.8.9.
10.11.12.
19.20.21.22.23.24.
BrainHeartKidneySpleenLiverColon
LungSmall IntestineMuscleStomachTestisPlacenta
Prostate GlandPBLFetal BrainFetal LiverFatMammary Gland
Fig. 2
Fig. 3
Fig. 4
1 2 3 4 5 6 7 8 9 10 11 12
13 14 15 16 17 18 19 20 21 22 23 24
1 2 3 4 5 6 7 8 9 10 11 12
13 14 15 16 17 18 19 20 21 22 23 24
5x
1x
5x
1x
5x
1x
5x
1x
5x
1x
5x
1x
Fig. 5
SR-10
2
2
8
8
4
4
A
B
C
D
E
F
G
H
1 2 3 4 5 6 7 8 9 10 11 12
1
1
2
2
3
3
4
4
5
5
6
6
7
7
8
8
9
9
10 11 12
13 14 15 16 17 18
19 20 22 23 24
10 11 12
13 14 15 16 17 18
21
19 20 22 23 2421
1
1
2
2
3
3
4
4
5
5
6
6
7
7
8
8
9
9
10 11 12
13 14 15 16 17 18
19 20 22 23 24
10 11 12
13 14 15 16 17 18
21
19 20 22 23 2421
Fig. SR-10
1.2.3.4.5.6.
13.14.15.16.17.18.
7.8.9.
10.11.12.
19.20.21.22.23.24.
BrainHeartKidneySpleenThymusLiver
Adrenal GlandSalivary GlandUterusProstate GlandEmbryo 8-9 dayEmbryo 9.5 day
StomachSmall IntestineMuscleLungTestisSkin
Embryo 12.5 dayEmbryo 19 dayBreast/VirginBreast/PregnantBreast/LactatingBreast/Involuting
5x
1x
5x
1x
5x
1x
5x
1x
Full-
len
gth
cDN
Acl
on
ing
Sure
-RAC
E™5’
-RAC
Eki
t
80 www.origene.com
results show a single major band of the appropriate
size in most tissues.
The Sure-RACE™ panel can also be used to identify
differentially-spliced transcripts that contain
alternate 5’ exons that are utilized in different tissues
(Fig. 7). The fibroblast growth factor 1 (FGF-1) gene
makes use of different 5’ exons to regulate its
expression.
Fig. 7
Fig. 6
1 2 3 4 5 6 7 8 9 10 11 12
13 14 15 16 17 18 19 20 21 22 23 24
1 2 3 4 5 6 7 8 9 10 11 12
13 14 15 16 17 18 19 20 21 22 23 24
5X
5X
Full-
len
gth
cDN
Acl
on
ing
DupL
EX-A
™ye
astt
wo-
hybr
idsy
stem
81ph: 1.888.267.4436 fax: 1.301.340.9254
Yeast Strains
EGY48 Contains 6 LexA binding sites upstream of LEU2 reporter gene; most sensitive
EGY194 Contains 4 LexA binding sites upstream of LEU2 reporter gene; less sensitive
EGY188 Contains 2 LexA binding sites upstream of LEU2 reporter gene; least sensitive
EGY40 Contains 0 LexA binding sites upstream of LEU2 reporter gene; control strain
RFY206 Used for specificity testing in a mating assay
E. coli Strains
KC8 trp- strain for recovery of plasmids
Expression Plasmids
pEG202 For constitutive expression of LexA-bait fusion protein (Fig. 2 on Page 84)
pEG202-NLS For constitutive expression of LexA-NLS-bait fusion protein
pJG4-5 Galactose-inducible expression of NLS-B42-HA tag-target protein (Fig. 2 on Page 84)
pJG4-6 Galactose-inducible expression of HA tag-target protein
pNLexA Used for fusing LexA to the C-terminus rather than the N-terminus of the bait
LacZ Plasmids
pSH18-34 Contains 8 LexA binding sites upstream of LacZ reporter gene; most sensitive
pJK103 Contains 2 LexA binding sites upstream of LacZ reporter gene; less sensitive
pRB1840 Contains 1 LexA binding site upstream of the LacZ reporter gene; least sensitive
pJK101 Used for testing the ability of the LexA fusion protein to enter the nucleus
Product Listing Cat. No. PriceDupLEX-A™ yeast two-hybrid system DKT-100 1800includes all items below
DupLEX-A™ yeast two-hybrid system
Full-
len
gth
cDN
Acl
on
ing
DupL
EX-A
™ye
astt
wo-
hybr
idsy
stem
Control Plasmids
pSH17-4 Positive control; activates reporter genes on its own
pRHFM1 Negative control; does not interact with positive target
pLexA-Max Negative control; does not interact with positive target
pBAIT LexA-bait fusion protein which interacts with pTARGET fusion protein
pTARGET B42-HA tag-target fusion protein which interacts with pBAIT fusion protein
Primers
5’ Bait Primer Primer for sequencing vector-insert junction in pEG202
5’ Target Primer Primer for PCR and sequencing in pJG4-5
3’ Target Primer Primer for PCR and sequencing in pJG4-5
82 www.origene.com
Introduction
One objective of studying a particular biochemical
pathway or process is to determine all of the
molecular components involved and define how they
interact with one another.The increasing pace of gene
discovery is providing researchers with a multitude of
genes whose functions have yet to be determined.
Based upon their translated sequences, individual
gene products may be assigned to specific classes of
proteins such as kinases or phosphatases, but this
type of analysis does not provide information on what
specific role they play in the cell. However, knowing
what protein(s) they interact with may provide insight
into which biochemical pathway they belong, and
hence what their function(s) may be.
Pioneered by Fields and Song in 19891 and Gyuris et
al. in 19932, the yeast two-hybrid system is a method
for detecting protein-protein interactions in vivo. The
yeast two-hybrid system relies on the finding that
some transcriptional activator proteins have
separable DNA binding and transcriptional activation
domains. Neither domain can activate transcription
on its own and must be tethered to on another to
activate transcription. Interaction between two
proteins can be tested by fusing one with the DNA
binding domain and the other with the
transcriptional activation domain of the activator
protein. If the two proteins interact, then the
transcriptional activator is reconstituted and can
bind to its binding site and activate transcription of a
reporter gene. Yeast is used since it is eukaryotic, can
be genetically modified easily, and can be
transformed. The yeast Gal4 protein and the E. coli
LexA protein are two transcriptional activators that
have been used to develop such yeast two-hybrid
systems.
The yeast two-hybrid system can be used to test for
interactions between two known proteins, but its
greatest utility has been in screening cDNA
expression libraries for proteins that will interact
with the specific bait being tested. Libraries have
been constructed from many different organisms,
tissues, and cell lines for screening by the yeast two-
1. A novel genetic system to detect protein-protein interactions.Nature. 1989 Jul 20;340(6230):245-6.
2. Cdi1, a human G1 and S phase protein phosphatase that associates with Cdk2.Cell. 1993 Nov 19;75(4):791-803.
hybrid system. The chances of successfully finding
an interacting partner rely heavily on choosing the
right tissue or cell-type for the protein of interest, as
well as on the quality of the library itself. The yeast
two-hybrid system and variations thereof are
becoming increasingly popular tools in gene
discovery and functional studies.
Product Description
The DupLEX-A™ Yeast Two-Hybrid System exploits
the fact that an E. coli transcriptional activation
domain, B42, and an E. coli DNA binding protein,
LexA, can activate transcription of a reporter when
LexA is bound to its DNA recognition sequence and
is also tethered to the B42 activation domain. Neither
the LexA protein nor the B42 activation domain
alone can activate transcription. The two-hybrid
system involves fusing the LexA protein with a bait
protein “X” and the B42 activation domain with a
target protein “Y” and co-expressing them in yeast
containing a reporter gene with LexA binding sites
inserted into its promoter. If “X” and “Y” interact,
then the B42 activation domain is anchored to the
promoter of the reporter gene through the LexA
protein and transcriptional activation of the reporter
can be detected (Fig. 1). In the DupLEX-A™ system,
“X” is the bait and “Y” is a library of proteins.
Full-
len
gth
cDN
Acl
on
ing
DupL
EX-A
™ye
astt
wo-
hybr
idsy
stem
83ph: 1.888.267.4436 fax: 1.301.340.9254
ADVANTAGESof the DupLEX-A™ System over other LexA or Gal4-based yeast
two-hybrid systems
Ability to eliminate false positives since target protein expression
is inducible
Ability to screen potentially toxic target proteins
Reporters with varying sensitivities are available so that baits which
activate transcription on their own canpotentially still be assayed by using
a less sensitive reporter
Allows co-immunoprecipitation of interacting proteins using antibodies
to HA tag or LexAp
Easy assays are available to determine whether or not a particular
bait protein can enter the yeast nucleus and bind LexA operators
Libraries are unamplified and are provided in the form of
ready-to-use DNA
Fig. 1
Full-
len
gth
cDN
Acl
on
ing
DupL
EX-A
™ye
astt
wo-
hybr
idsy
stem
The bait vector pEG202 and the target or library
vector pJG4-5 are shown in Fig. 2.
DupLEX-A™ yeast two-hybrid system libraries
OriGene has an extensive list of cDNA libraries
available for use with the DupLEX-A™ Yeast Two-
Hybrid System kit. For each library, oligo(dT)-primed
cDNA was synthesized and cloned directionally
between the EcoRI and XhoI sites of pJG4-5, the
activation domain expression vector used in the
DupLEX-A™ system. After plating the primary
bacterial transformants, the cells were scraped from
the plates, pooled, an aliquot was removed and
frozen away, and plasmid DNA was purified from the
rest. Approximately 100 µg of purified, ready-to-use
library DNA is provided. Thus the researcher need not
perform the laborious task of library DNA isolation.
Moreover, the provided library DNA has never been
amplified.
A frozen E. coli glycerol stock of the plasmid library is
also provided which can be readily used to prepare
additional DNA, if needed.
The list of libraries includes those made from specific
human, rat, and mouse tissues, as well as from whole
organisms. In addition, a special emphasis has been
made on constructing libraries from several breast
and prostate cancer tissues and cell lines.
It should be noted that these libraries, while designed
to complement the yeast two-hybrid system, may also
be used as standard plasmid libraries for isolation of
cDNA clones using conventional nucleic acid-based
screening strategies.
84 www.origene.com
GAA TTC CCG GGG ATC CGT CGA CCA TGG CGG CCG CTC GAG TCG ACC TGC AGCEco RI Bam HI Sal I Nco l Not l Xho l Sal l
Amp R
pBR322ori
ADH promLexA ADH
term
2 micron
HIS3
TRP1
ADHterm
2 micron
Amp R
pUC ori
GAL prom
B42-HA tag
nucl. local. B42 acid blob domain HA tag
ATG EcoRV EcoRI XhoI
Amp R
pEG202(10.2 kb)
pJG4-5(6.4 kb)
Fig. 2
Human
Human Fetal Brain* 3.5 x 106 0.2 – 1.6 kb DLH-101 1250(Frontal Cortex, 22-week)
Human Fetal Kidney 3.0 x 106 0.2 – 2.0 kb DLH-106 1250
Human Liver (Adult) 1.1 x 107 0.3 – 4.8 kb DLH-100 1250
Human Fetal Liver 8.6 x 106 0.4 – 2.5 kb DLH-105 1250
Human Ovary(Adult) 4.0 x 106 0.4 – 6.1 kb DLH-114 1250
Human PBL 1.0 x 107 0.3 – 2.5 kb DLH-104 1250(Peripheral Blood Leukocytes)
HeLa Cell* Line 9.6 x 106 0.3 – 3.3 kb DLH-103 1250
Jurkat T-cell Line 5.7 x 106 0.3 – 4.0 kb DLH-115 1250
MG63 Cell Line 3.0 x 106 0.3 – 2.5 kb DLH-118 1250(Osteosarcoma Cell Line)
WI-38 Cell Line 5.7 x 106 0.4 – 5.0 kb DLH-102 1250(Human Lung Fibroblast)*(Serum-starved)
SKOV3 Cell Line 5.0 x 106 0.2 – 2.3 kb DLH-111 1250(Ovarian Cancer Cell Line)
Mouse
Mouse Brain (Adult) 6.1 x 106 0.3 – 1.7 kb DLM-100 1250
Mouse Embryo (Whole, 19-day) 1.0 x 107 0.2 – 2.5 kb DLM-110 1250
Mouse Liver (Adult) 9.5 x 106 0.3 – 3.2 kb DLM-102 1250
Mouse Ovary (Adult) 4.0 x 106 0.3 – 1.5 kb DLM-103 1250
Mouse Skeletal Muscle (Adult) 7.2 x 106 0.4 – 3.5 kb DLM-111 1250
Mouse Spleen (Adult) 1.0 x 107 0.2 – 2.5 kb DLM-101 1250
Full-
len
gth
cDN
Acl
on
ing
Yeas
ttw
o-hy
brid
expr
essi
oncD
NA
libra
ries
85ph: 1.888.267.4436 fax: 1.301.340.9254
Product ListingRNA Source # of Indt. Insert Size Cat. No. Price
Clones Range
Yeast two-hybrid expression cDNA libraries
Full-
len
gth
cDN
Acl
on
ing
Yeas
ttw
o-hy
brid
expr
essi
oncD
NA
libra
ries
86 www.origene.com
RNA Source # of Indt. Insert Size Cat. No. PriceClones Range
Rat
Rat Adipocyte 1.0 x 107 0.4 – 5.0 kb DLR-103 1250(9 week-old Zucker rat)
Rat Brain (Adult) 4.5 x 106 0.3 – 3.4 kb DLR-102 1250
Rat Testis (Adult) 8.0 x 106 0.5 – 4.0 kb DLR-101 1250
Rat Thymus (Adult) 8.2 x 106 0.3 – 4.5 kb DLR-100 1250
Breast-specific
MCF7 Cell 1.0 x 107 0.6 – 4.5 kb DLH-112 1250(Estrogen-Depleted)
MCF7 Cell (Serum-grown) 1.0 x 107 0.4 – 3.5 kb DLH-117 1250
MCF7 Cell (Estrogen-treated) 1.0 x 107 0.4 – 4.6 kb DLH-113 1250
SKBR3 Cell (Estrogen- 5.4 x 106 0.5 – 4.0 kb DLH-116 1250receptor negative)
Mouse Breast, Normal 1.0 x 107 0.4 – 5.5 kb DLM-107 1250(Virgin)
Mouse Breast, Normal 6.3 x 106 0.4 – 5.3 kb DLM-108 1250(Pregnant, 12-Day)
Mouse Breast, Normal 1.0 x 107 0.4 – 3.1 kb DLM-105 1250(Lactating)
Mouse Breast, Normal 1.0 x 107 0.4 – 7.0 kb DLM-106 1250(Involuting)
Prostate-specific
Human Prostate, Normal 6.4 x 106 0.3 – 1.7 kb DLH-107 1250(Pooled from 8 adults)
Human Prostate, Tumor 4.8 x 106 0.2 – 2.0 kb DLH-108 1250(Pooled from 8 adults)
LNCaP Cell (Untreated) 2.9 x 106 0.3 – 2.0 kb DLH-109 1250
LNCaP Cell (Treated with 30 4.6 x 106 0.3 – 2.3 kb DLH-110 1250nM R1881)
Mouse Prostate, Normal 3.7 x 106 0.3 – 4.0 kb DLM-104 1250
Full-
len
gth
cDN
Acl
on
ing
Yeas
ttw
o-hy
brid
expr
essi
oncD
NA
libra
ries
87ph: 1.888.267.4436 fax: 1.301.340.9254
RNA Source # of Indt. Insert Size Cat. No. PriceClones Range
Other
C. elegans (Adult) 3.8 x 106 0.2 – 3.6 kb DLCE-100 1250
D. melanogaster (Adult) 1.8 x 106 0.3 – 1.8 kb DLDM-100 1250
S. cerevisiae*** (Genomic) 4.0 x 106 0.3 – 4.0 kb DLY-100 1250
MDBK Cell (Bovine Kidney) 5.8 x 106 0.3 – 1.7 kb DLBK-100 1250
*The libraries marked with a single asterisk (*) were amplified an additional time and plasmid DNA was purified from the re-amplified cells.**The average size of the inserts was calculated by restriction digestion analysis of plasmid DNA from 48 randomly-chosen colonies from each library. Please note that smaller insertsare more informative than larger inserts in the yeast two-hybrid system screen. Even so, there are larger inserts present in these libraries.***The S. cerevisiae library is from strain S288C, with inserts cloned into the EcoRI site of pJG4-5.
TRP1
ADHterm
2 micron
Amp R
pUC ori
GAL prom
B42-HA tag
nucl. local. B42 acid blob domain HA tag
ATG EcoRV EcoRI XhoI
Amp R
pJG4-5(6.4 kb)
Fig. 1
Introduction
OriGene provides an extensive list of expression
cDNA libraries. As a pivotal component of OriGene’s
DupLEX-A Yeast Two-Hybrid System kit, these
libraries has been utilized extensively by scientists
and were cited in numerous publications1-11. For each
library, oligo(dT)-primerd cDNA was synthesized
from high quality mRNAs and cloned uni-
directionally between EcoRI and XhoI site of pJG4-5
(Fig. 1), the activation domain expression vector
used in the DupLEX-A system. After plating the
primary bacterial transformants, the cells were
scraped from the plates, pooled, an aliquot was
removed and frozen away and the plasmid was
purified from the rest. Thus the researchers need not
perform the laborious task of library DNA isolation.
Moreover, the provided library DNA has never been
amplified.
Full-
len
gth
cDN
Acl
on
ing
Yeas
ttw
o-hy
brid
expr
essi
oncD
NA
libra
ries
88 www.origene.com
In addition, while designed to complement the yeast
two-hybrid system, these libraries can be used as
standard plasmid libraries for isolation of cDNA
clones using conventional nucleic acid-based
screening strategies.
The list of libraries includes those made from specific
human, rat, and mouse tissues, as well as from
whole organisms. In addition, a special emphasis
has been made on constructing libraries from
several breast and prostate cancer tissues and cell
lines.
Product Description:
For each library, oligo(dT)-primed cDNA was
synthesized and cloned directionally between the
EcoRI and XhoI sites of pJG4-5. After plating the
primary bacterial transformants, the cells were
scraped from the plates, pooled, an aliquot was
removed and frozen away, and plasmid DNA was
purified from the rest. Approximately 100 µg of
purified, ready-to-use library DNA is provided. Thus
the researcher need not perform the laborious task of
library DNA isolation. Moreover, the provided library
DNA has never been amplified.
A frozen E. coli glycerol stock of the plasmid library is
also provided which can be readily used to prepare
additional DNA, if needed.
It should be noted that these libraries, while designed
to complement the yeast two-hybrid system, can also
be used as standard plasmid libraries for isolation of
cDNA clones using conventional nucleic acid-based
screening strategies.
ADVANTAGES
Supplied as ready-to-use library DNA plus bacterial
glycerol stock
Largest selection of cDNA libraries for TRP1-compatible yeast
two-hybrid systems
Several libraries designed specifically for breast and prostate
cancer research
1. Coordinated functions of WSS1, PSY2 and TOF1 in the DNA damage response.Nucleic Acids Res. 2004 Dec 14;32(22):6519-30.2. GIPC recruits GAIP (RGS19) to attenuate dopamine D2 receptor signaling.Mol Biol Cell. 2004 Nov;15(11):4926-37.3.Ribosomal S6 kinase as a mediator of keratinocyte growth factor-induced activation ofAkt in epithelial cells.Mol Biol Cell. 2004 Jul;15(7):3106-13.4. Small interfering RNAs mediate sequence-independent gene suppression and induceimmune activation by signaling through toll-like receptor 3.J Immunol. 2004 Jun 1;172(11):6545-9.5. Murine tenascin-W: a novel mammalian tenascin expressed in kidney and at sites ofbone and smooth muscle development.J Cell Sci. 2004 Feb 1;117(Pt 4):571-81.6. p57Kip2 regulates actin dynamics by binding and translocating LIM-kinase 1 to thenucleus.J Biol Chem. 2003 Dec 26;278(52):52919-23.
7. An interaction between a MYC protein and an EREBP protein is involved intranscriptional regulation of the rice Wx gene.J Biol Chem. 2003 Nov 28;278(48):47803-11.8. PDZ Domain-mediated interaction of interleukin-16 precursor proteins with myosinphosphatase targeting subunits.J Biol Chem. 2003 Oct 24;278(43):42190-9.9. The Lafora disease gene product laforin interacts with HIRIP5, a phylogeneticallyconserved protein containing a NifU-like domain.Hum Mol Genet. 2003 Sep 15;12(18):2359-68.10. Candida albicans Ssa1/2p is the cell envelope binding protein for human salivaryhistatin 5.J Biol Chem. 2003 Aug 1;278(31):28553-61.11. Fas-associated death domain protein interacts with methyl-CpG binding domainprotein 4: a potential link between genome surveillance and apoptosis.Proc Natl Acad Sci U S A. 2003 Apr 29;100(9):5211-6.
Gene expressionRapid-Scan™: PCR-based gene expression profiling panel
Real-Time-Scan™: Real-Time PCR-based gene expression profiling panel
Northern Blot
Multiple-Choice™ cDNAs
Blue-Ribbon™ Poly A+ and Total RNAs
Gen
eex
pre
ssio
nRa
pid-
Scan
90 www.origene.com
Product Listing Cat. No. Price
Human Tissue Rapid-Scan Panel (24 Tissues) HSCA-101 675
Human Brain Rapid-Scan Panel (12 Parts) BSCD-101 520
Human Breast Cancer Rapid-Scan Panel TSCE-101 675(24 Normals/Tumors)
Mouse Tissue Rapid-Scan Panel (24 Tissues) MSCB-101 550
Mouse Brain Rapid-Scan Panel (48 Parts/Stages) NSCF-101 675
Drosophila Tissue Rapid-Scan Panel (12 Parts/Stages) DSCC-101 375
Human Tissue Real-Time Rapid-Scan Panel HSCR-101 (<5) 680(48 Tissues) New!
HSCR-101 (5–10) 575
HSCR-101 (>10) 500
Rapid-Scan
Gen
eex
pre
ssio
nRa
pid-
Scan
91ph: 1.888.267.4436 fax: 1.301.340.9254
Introduction
Rapid-Scan™ is a PCR-based system, using high-
quality, first-strand cDNAs derived from different
tissues and/or developmental stages, to generate a
"comprehensive" expression profile of any cloned
gene or EST from either human, mouse or Drosophila.
Individual first-strand cDNAs have been tested to
assure that low abundance and long transcripts are
represented, and have been normalized using an
internal standard. The cDNAs were then serially-
diluted over a 4-log range and arrayed in a 48- or 96-
well PCR plate. This ensures that the amplification
reaction will be within the linear range and, hence, will
facilitate semi-quantitative determination of relative
mRNA accumulation in the various tissues and
developmental stages. With this non-radioactive
technique, one can generate a comprehensive
expression profile for any given gene in just three
hours. All that is required is a 48- or 96-well PCR using
gene-specific primers, followed by electrophoretic
analysis of the products in an agarose gel.
The Rapid-Scan Gene Expression Panels are highly
sensitive and provide a quick readout about the sites
of transcript accumulation. If more exact quan-
tification and sizing of transcript are desired, this can
be accommodated by use of Northern RNA Blots.
To take advantage of Real-Time PCR technology and
to provide more accurate profiling of gene
expression, OriGene launched the new Real-Time
Rapid-Scan panel. This panel is based on the same
principle, yet the cDNA template was normalized by
real-time PCR. The advantage of Real-Time panel
over the conventional Rapid-Scan is as follows.
1. Real-time PCR platform compatible
2. Wider tissue spectrum: 48 tissue in Real-time panel
vs. 24 tissues in conventional panel
3. More accurate quantification of gene expression
level
4. Simplified procedure: no more agarose gel
electrophoresis and gel imaging steps.
ADVANTAGES
Fast and non-radioactive
Simultaneous examination ofmany different tissues and/or
developmental stages
Highly sensitive andsemi-quantitative
Simultaneous analysis ofalternatively-spliced transcripts
Real-time PCR compatibility with newly-launched 48-tissue
human panel
Gen
eex
pre
ssio
nRa
pid-
Scan
92 www.origene.com
Product Description:
Rapid-Scan™ Gene Expression Panels were
assembled by selecting either 24 frequently-studied
human tissues, 12 human brain parts, 24 human
breast cancer (normal/tumor) samples, 24 major
mouse tissues, 48 mouse brain parts and stages, or
12 Drosophila tissues and stages. To avoid detection
of individual differences in gene expression, tissues
were pooled, whenever possible, from multiple
individuals.
Total RNA was isolated and subjected to oligo (dT)
selection. The recovered poly A+ RNA was then
examined by Northern blot hybridization to confirm
RNA integrity. The poly A+ RNA was then used to
synthesize first-strand cDNA, using oligo (dT)
primers and MMLV reverse transcriptase. Individual
cDNAs were confirmed to be free of genomic DNA
contamination and to contain complete reverse
transcripts of selected rare and long mRNAs.
The first-strand cDNAs from each tissue were then
subjected to normalization, such that they all contain
an equivalent concentration of a control reverse
transcript. Each cDNA was diluted in water to a series
of four concentrations (labeled 1000x, 100x, 10x and
1x), with the lowest concentration (1x) being
approximately 1 pg. The diluted cDNAs were
subsequently arrayed in a 48- or 96-well PCR plate.
Six conventional Rapid-Scan™ Gene Expression
Panels are currently available: 24 human tissues
(normalized against actin), 12 human brain parts
(normalized against actin), 24 human breast cancer
(normal/tumor) samples (normalized against actin),
24 major mouse tissues (normalized against actin),
48 mouse brain parts and stages (normalized against
actin), and 12 Drosophila tissues and stages
(normalized against rp49).
Real-Time Rapid-Scan is a new member of the Rapid-
Scan family. It is a real-time PCR ready assembly of
high-quality cDNAs from 48 human tissues and the
cDNA was normalized against beta-actin using real-
time PCR. There is no need for serial dilution
therefore only one concentration of (approximately
1ng) of cDNA is provided. The customer can simply
add the real-time PCR mix with the gene specific
primers and obtain an accurate gene expression
profile across 48 tissues with minimal hand-on time.
PRODUCT COMPONENTS
Two identical Rapid-Scan™ 48- or 96-well PCR plates containing dried, serially-diluted, PCR-ready,
first-strand cDNAs
Two adhesive cover sheetsfor sealing the PCR plates
Primers for detection ofcontrol cDNA
Gen
eex
pre
ssio
nRa
pid-
Scan
93ph: 1.888.267.4436 fax: 1.301.340.9254
4 Document the results by
imaging/photography.
The Rapid-Scan™ Gene Expression
Panels are easy to use and require a
minimal amount of time to obtain a
complete expression profile for a
given gene.
2 Place the plate
into a thermal
cycler and
proceed with
PCR.
3 Upon completion of the PCR, analyze
the reaction products by agarose gel
electrophoresis.
Product Use
1 Prepare a 48-well or 96-well PCR
master mix including two gene-
specific primers for the cDNA of
interest and aliquot the solution
into each well of the Rapid-Scan™
PCR plate.
Gen
eex
pre
ssio
nRa
pid-
Scan
94 www.origene.com
Rapi
d-Sc
an
Product Application
A. Human Tissue Rapid-Scan™ Panel
The Human Rapid-Scan™ Gene Expression Panel
consists of cDNAs derived from 24 different tissues.
The cDNAs were arrayed in a 96-well PCR plate in the
order indicated in Fig. 1.
To facilitate comparison of transcript accumulation in
different tissues, the first-strand cDNAs have been
normalized using actin as an internal control.
Amplification of actin cDNA (using primers provided
with the Rapid-Scan™ panels) shows equivalent
distribution in all 24 human tissues (Fig. 2).
The Rapid-Scan™ panel was used to determine the
relative accumulation of the muscle-specific a-actinin
2 transcript, which is detected predominantly in
skeletal muscle and heart, but also in brain.
The Human Rapid-Scan™ panel was used to
generate expression profiles of selected previously-
known genes in various tissues or differentiation
states. Myelin-associated glycoprotein (MAG) was
detected only in adult brain, adenosine mono-
phosphate deaminase 1 (isoform 1) (AMPD1) only in
muscle, thyroglobulin (TG) only in thyroid gland,
serine protease 3 (mesotrypsin) (PRSS3) only in
pancreas, and protein tyrosine phosphatase (PTPN1)
only in prostate gland.
B. Human Brain Rapid-Scan™ Panel
The Human Brain Rapid-Scan™ Gene Expression
Panel consists of cDNAs derived from 12 different
brain parts. The RNA was prepared and pooled from
two individuals. The cDNAs were normalized against
actin and arrayed in a 48-well PCR plate in the order
indicated in Fig. 3.
This panel was used to determine the relative
transcript levels of a gene family. Three members of
the human dopamine receptor family were analyzed
and the PCR products were separated in an agarose
gel (Fig. 4).
Fig. 1
1000X
100X
10X
1X
1000X
100X
10X
1X
1000X
1000X
1000X
1000X
1000X
MAG
AMPD1
TG
PRSS3
PTPN1
α-actinin 2
actin
M 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
A
B
C
D
E
F
G
H
1000x
100x
10x
1x
1000x
100x
10x
1x
1. Brain2. Heart3. Kidney4. Spleen5. Liver6. Colon7. Lung8. Small Intestine
9. Muscle10. Stomach11. Testis 12. Placenta13. Salivary Gland14. Thyroid Gland15. Adrenal Gland16. Pancreas
17. Ovary18. Uterus19. Prostate Gland20. Skin21. PBL22. Bone Marrow23. Fetal Brain24. Fetal Liver
Fig. 2
1 2 3 4 5 6 7 8 9 10 11 12
13 14 15 16 17 18 19 20 21 22 23 24
Fig. 3
1 2 3 4 5 6 7 8 9 1 0 1 1 1 2
1. Frontal Lobe2. Temporal Lobe3. Cerebellum4. Hippocampus5. Substantia Nigra6. Caudate Nucleus
7. Amygdala8. Thalamus9. Hypothalamus
10. Pons11. Medulla12. Spinal Cord
A
B
C
D
1000x
100x
10x
1x
Gen
eex
pre
ssio
nRa
pid-
Scan
95ph: 1.888.267.4436 fax: 1.301.340.9254
The D5 dopamine receptor was present at more or
less equal levels in all of the brain parts examined.
The D2 receptor also showed broad distribution but
at varying levels of accumulation. On the other hand,
the D1 receptor was only expressed in the caudate
nucleus, verifying results that have been previously
observed. The results also demonstrate equivalent
levels of actin in each normalized tissue.
C. Human Breast Cancer Rapid-Scan™ Panel
The Human Breast Cancer Rapid- Scan™ Gene
Expression Panel consists of cDNAs derived from 12-
normal and 12-tumor breast tissues. The normal
tissues were obtained by reduction mammaplasty
and microdissected to remove fat tissues. The tumor
tissues were from patients with carcinoma of the
breast. The cDNAs were normalized against actin and
arrayed in a 96-well PCR plate in the order indicated
in Fig. 5.
This panel is designed to examine the change in
gene expression of a candidate gene between nor-
mal and tumor tissues. The expression of 6 genes
related in breast cancer was examined using this
panel. (Fig. 6)
Genes 1, 2, and 3 were up-regulated in many breast
tumor tissues and undetectable in normal breast
tissues. In contrast, the expression of genes 4, 5, and
6 were down-regulated in many breast tumor tissues
and almost undetectable in normal breast tissues.
Fig. 4
10X
1000X
1000X
1000X
actin
D1
D2
D5
1 2 3 4 5 6 7 8 9 10 11 12
1. Normal Breast 12. Normal Breast 23. Normal Breast 34. Normal Breast 45. Normal Breast 56. Normal Breast 67. Normal Breast 78. Normal Breast 89. Normal Breast 9
10. Normal Breast 1011. Normal Breast 1112. Normal Breast 12
13. Breast Cancer 114. Breast Cancer 215. Breast Cancer 316. Breast Cancer 417. Breast Cancer 518. Breast Cancer 619. Breast Cancer 720. Breast Cancer 821. Breast Cancer 922. Breast Cancer 1023. Breast Cancer 1124. Breast Cancer 12
A
B
C
D
E
F
G
H
1000x
100x
10x
1x
1000x
100x
10x
1x
Fig. 5
Fig. 6
1000 X1000 X1000 X1000 X
1000 X
1000 X
1000 X
1000 X
actin
Gene 1
Gene 2
Gene 3
Gene 4
Gene 5
Gene 6
M 1 2 3 4 5 6 7 8 9 10 11 12 M 13 14 15 16 17 18 19 20 21 22 23 24
1 2 3 4 5 6 7 8 9 10 11 12
13 14 15 16 17 18 19 20 21 22 23 24
Gen
eex
pre
ssio
nRa
pid-
Scan
96 www.origene.com
D. Mouse Tissue Rapid-Scan™ Panel
The Mouse Rapid-Scan™ Gene Expression Panel
consists of cDNAs derived from 24 different tissues
or developmental stages. The adult tissues were
from outbred Swiss Webster mice, and the breast
tissues from outbred CD1 mice. The cDNAs were
normalized against actin and arrayed in a 96-well
PCR plate in the order indicated in Fig. 7.
The Rapid-Scan™ panel is an effective tool to obtain
the expression profile of an uncharacterized EST or
to confirm tissue-specific expression. The results
shown in Fig. 8 demonstrate such an application.
The Mouse Rapid-Scan™ panel was used to confirm
the expression profiles for a mouse heart-specific
gene (Tbx20) and a skin-specific EST sequence. The
heart-specific gene (Tbx20) was found to be
expressed in abundance in heart and at a lower level
in embryo at different stages of gestation. The skin-
specific EST sequence was found to be expressed
not only in skin, but also in stomach and in day 19
embryos.
E. Mouse Brain Rapid-Scan™ Panel
The Mouse Brain Rapid-Scan™ Gene Expression
Panel consists of cDNAs derived from 48 mouse
brain parts at five developmental stages. The brain
tissues were harvested from Outbred NIH Swiss
mice. The cDNAs were normalized against actin and
arrayed in a 96-well PCR plate in the order indicated
in Fig. 9.
This panel has been used to profile the expression of
mouse DLX-5 (Drosophila homolog Distal-less 5),
dopamine receptor DD3 and Hox3.1. The results of
Hox3.1 are shown in Fig. 10.
In embryonic stages, Hox3.1 is expressed in many
brain parts with a high level of expression in the
spinal cord. However, in adults, its expression is
restricted to the spinal cord. This piece of data
demonstrates the usefulness of this panel in
providing clues to understanding potential gene
functions.
1000 X
100 X
10 X
1 X
1000 X
100 X
10 X
1 X
1000 X
100 X
10 X
1 X
actin
Tbx20
Skin-specific EST
M 1 2 3 4 5 6 7 8 9 10 11 12 M 13 14 15 16 17 18 19 20 21 22 23 24
Fig. 7
Fig. 8
1. Brain2. Heart3. Kidney4. Spleen5. Thymus6. Liver7. Stomach8. Small Intestine
9. Muscle10. Lung11. Testis 12. Skin13. Adrenal Gland14. Pancreas15. Uterus16. Prostate Gland
17. Embryo/8.5 day18. Embryo/9.5 day19. Embryo/12.5 day20. Embryo/19 day21. Breast/Virgin22. Breast/Pregnant23. Breast/Lactating24. Breast/Involuting
A
B
C
D
E
F
G
H
1000x
100x
10x
1x
1000x
100x
10x
1x
1 2 3 4 5 6 7 8 9 10 11 12
13 14 15 16 17 18 19 20 21 22 23 24
Gen
eex
pre
ssio
nRa
pid-
Scan
97ph: 1.888.267.4436 fax: 1.301.340.9254
F. Drosophila Tissue Rapid-Scan™ Panel
The Drosophila Rapid-Scan™ Gene Expression
Panel consists of cDNAs derived from 12 different
tissues and developmental stages. The RNA was pre-
pared from Drosophila melanogaster Canton S
strain. The cDNAs were then arrayed in a 48-well PCR
plate in the order indicated in Fig. 11. This was used
to confirm the expression profiles of genes whose
sites of expression have already been established.
The results are shown in Fig. 12.
The expression of the actin genes in Drosophila is
developmentally regulated, therefore, rp49, the gene
encoding a ribosomal protein, was used as an
internal control to normalize each Drosophila cDNA.
The male-specific Fruitless transcript was only
detected in the adult male head. The alternatively-
Fig. 9
Embryo day 131. Telencephalon/
Diencephalon2. Mesencephalon
(Midbrain)3. Rhombencephalon
(Hindbrain)4. Spinal cord
Embryo day 155. Telencephalon6. Diencephalon7. Midbrain8. Pons9. Medulla
10. Spinal cord
Embryo day 1811. Frontal cortex12. Posterior cortex13. Entorhinal cortex
14. Olfactory bulb15. Hippocampus16. Striatum17. Thalamus18. Hypothalamus19. Midbrain20. Pons21. Medulla22. Spinal cord
Postnatal day 7 23. Frontal cortex24. Posterior cortex25. Entorhinal cortex26. Olfactory bulb27. Hippocampus28. Striatum29. Thalamus30. Hypothalamus31. Cerebellum
32. Midbrain33. Pons34. Medulla35. Spinal cord
Adult 5 week36. Frontal cortex37. Posterior cortex38. Entorhinal
cortex39. Olfactory bulb40. Hippocampus41. Striatum42. Thalamus43. Hypothalamus44. Cerebellum45. Midbrain46. Pons47. Medulla48. Spinal cord
A
B
C
D
E
F
G
H
1000x
100x
1000x
100x
1000x
100x
1000x
100x
1 2 3 4 5 6 7 8 9 10 11 12
13 14 15 16 17 18 19 20 21 22 23 24
25 26 27 28 29 30 31 32 33 34 35 36
37 38 39 40 41 42 43 44 45 46 47 48
Fig. 11
1. Embryo/0–4 hr2. Embryo/4–8 hr3. Embryo/8–12 hr4. Embryo/12–24 hr5. 1st Instar6. 2nd Instar
7. 3rd Instar8. Pupae9. Male Head
10. Female Head11. Male Body12. Female Body
A
B
C
D
1000x
100x
10x
1x
1 2 3 4 5 6 7 8 9 10 11 12
spliced male form of Doublesex was detected mainly
in the 3rd instar and in male tissues, and is not
present in female tissues. The Eyeless transcript was
detected in whole embryos, starting from 4 hours,
and in adult head, but not in adult body.
1-12
1-12
13-24
13-24
25-36
25-36
37-48
37-48
1000x
100x
1000x
100x
1000x
100x
1000x
100x
Fig. 10
1000X
1000X
1000X
1000X
1000X
1000X
1000X
Bicoid
Antennapedia
Eyeless
Ultrabithorax
Fruitless(male form)
Doublesex(male form)
Doublesex(female form)
1 2 3 4 5 6 7 8 9 10 11 12
Fig. 12
Gen
eex
pre
ssio
nRe
al-T
ime-
Scan
98 www.origene.com
F. Human Real-Time Rapid-Scan Panel
The Human Real-Time Rapid-Scan gene Expression
Panel consists of cDNAs derived from 48 different
tissues. The cDNAs were arrayed in a 96 well PCR
plate in the order indicated in Fig. 13.
The first-strand cDNAs have been normalized by
real-time PCR using beta-actin as an internal control.
Amplification of actin cDNA (using primers provided
with the Rapid-Scan panels shows equivalent
distribution in all 48 human tissues. (Fig. 14, Panel A)
To validate the panel for specificity and sensitivity,
the human Real-Time Rapid-Scan Panel was used to
generate expression profiles of selected previously-
known gene in various tissues (Fig. 14, Panel B-D).
Thyroglobulin was detected only in thryroid and
parathyroid. Skin-specific gene, Late cornified
envelope 2B (LCE2B) was detected only in nasal
mucosa and skin. Prostate specific antigen, kallikrein
3 (KLK3) was detected only in prostate.
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48
Beta-actin
thyroglobulin
LCE2B
KLK3
A
B
C
D
FIG. 14
1 2 3 4 5 6 7 8 9 1 0 1 1 1 2
A
B
C
D
1 Adrenal Gland2 Bile duct3 Bone marrow4 Brain5 Bronchus6 Cervix7 Colon8 Decending part
of duodenum9 Epididymis
10 Esophagus11 Fat12 Heart
13 Intestine (Small)14 Intracranial artery15 Kidney16 Larynx17 Liver18 Lung19 Lymph node20 Lymphocytes
(peripheral blood)21 Mammary gland22 Muscle23 Nasal mucosa24 Optic nerve
25 Ovary26 Oviduct27 Pancreas28 Parathyroid29 Pericardium30 Pituitary31 Placenta32 Prostate33 Rectum34 Salivary Gland35 Seminal vesicles36 Skin
37 Spleen38 Stomach39 Testis40 Thymus41 Thyroid42 Tonsil43 Ureter44 Urinary bladder45 Uterus46 Uvula47 Vagina48 Vena Cava
Row A Row B Row C Row D
Fig. 13
Gen
eex
pre
ssio
nN
orth
ern
Blot
99ph: 1.888.267.4436 fax: 1.301.340.9254
Product Listing Cat. No. PriceHuman Poly A+ RNA Blot I HB-2010 725Northern Blot — 12 Tissues(Brain, Lung, Heart, Muscle, Stomach, Small Intestine, Colon, Liver, Kidney, Spleen, Testis, Placenta)
Human Poly A+ RNA Blot II HB-2011 725Northern Blot — 12 Tissues(Brain, Duodenum, Esophagus, Pancreas, PBL, Prostate, Salivary Gland, Testis, Thymus, Thyroid, Urinary Bladder, Uterus )
Human Poly A+ RNA Blot III HB-1102 415Northern Blot — 6 Tissues(Salivary Gland, Pancreas, Adrenal Gland, Ovary, Uterus, Skin)
Mouse Poly A+ RNA Blot MB-2020 640Northern Blot — 12 Major Tissues(Brain, Lung, Heart, Muscle, Stomach, Small Intestine, Liver, Kidney, Spleen, Testis, Thymus, Skin)
Rat Poly A+ RNA Blot RB-2030 640Northern Blot — 12 Major Tissues(Brain, Thymus, Lung, Heart, Muscle, Stomach, Small Intestine, Liver, Kidney, Spleen, Testis, Skin)
Rat Total RNA Blot SB-2040 710Northern Blot — 12 Brain Parts (Frontal Cortex, Posterior Cortex, Entorhinal Cortex, Hippocampus, Olfactory Bulb, Striatum, Thalamus, Cerebellum, Midbrain, Pons, Medulla, Spinal Cord)
Northern blot
Introduction
Identification of tissues which express a gene of
interest is an essential first step for functional
analysis. While the Rapid-Scan™ Gene Expression
Panel is a highly sensitive and time-efficient system
to measure specific transcript accumulation, it does
not provide information about the size of the
transcript and is only semi-quantitative. The
workhorse in the industry, RNA blot hybridization or
Northern blotting, has been widely used for gene
expression analysis. Though often laborious,
Northern blotting provides, other than determination
of sites of expression, information regarding tran-
script size, RNA quantification, cross-hybridization
data, and alternate RNA splicing. In the standard
method, isolated total or poly A+ RNA from a variety
of tissues are separated by gel electrophoresis and
transferred onto a solid support. Hybridization of the
immobilized RNA with a labeled nucleic acid probe is
then performed to determine the relative levels of an
mRNA species in the various tissues.
Gen
eex
pre
ssio
nN
orth
ern
Blot
100 www.origene.com
Since 1997, OriGene has been providing pre-made
Northern blots to the research community. Its high
quality and reliability has been validated by citations
in many publications in peer-reviewed journals. The
citation list below is only a small sample of such
publications.
Product Description
The Northern Blots are premade, ready-to-hybridize
membranes offered in a variety of choices, in terms
of organism, tissue, and type of RNA. The integrity of
the RNA used in the blots is assured through a rigid
quality control process. Approximately 2 µg of
poly A+ RNA or 20 µg of total RNA per tissue sample
were separated in a denaturing agarose gel,
transferred onto a positively-charged nylon
membrane, and covalently crosslinked by UV light
irradiation. Loading of the poly A+ RNA from each
tissue was adjusted to reflect approximately equal
amounts of actin mRNA, while total RNA samples
were adjusted to reflect equivalent levels of
ribosomal RNA. Signal intensity for actin across each
tissue sample may vary slightly. Each blot contains
RNA markers for transcript size determination.
OriGene offers human RNA blots prepared from 12
tissues (Blot I and Blot II) or six tissues (Blot III).
OriGene also offers a mouse 12-major tissue blot, a
rat 12-major tissue blot, and a rat 12-brain part blot.
1. Cloning and characterization of mouse E2F8, a novel mammalian E2F family membercapable of blocking cellular proliferation.J Biol Chem. 2005 Feb 18.2. A targeted mutation of Nkd1 impairs mouse spermatogenesis.J Biol Chem. 2005 Jan 28;280(4):2831-9.3. dsu functions in a MYO5A-independent pathway to suppress the coat color of dilutemice.Proc Natl Acad Sci U S A. 2004 Nov 30;101(48):16831-6.4. Expression of slc5a8 in kidney and its role in Na(+)-coupled transport of lactate.J Biol Chem. 2004 Oct 22;279(43):44522-32.5. Targeted disruption of the Walker-Warburg syndrome gene Pomt1 in mouse results inembryonic lethality.Proc Natl Acad Sci U S A. 2004 Sep 28;101(39):14126-31.
6. Neonatal lethality, dwarfism, and abnormal brain development in Dmbx1 mutant mice.Mol Cell Biol. 2004 Sep;24(17):7548-58.7. TNAP, a novel repressor of NF-kappaB-inducing kinase, suppresses NF-kappaBactivation.J Biol Chem. 2004 Aug 20;279(34):35975-83. 8. A novel cardiolipin-remodeling pathway revealed by a gene encoding an endoplasmicreticulum-associated acyl-CoA:lysocardiolipin acyltransferase (ALCAT1) in mouse.J Biol Chem. 2004 Jul 23;279(30):31727-34.9. An inhibitory Ig superfamily protein expressed by lymphocytes and APCs is also anearly marker of thymocyte positive selection.J Immunol. 2004 May 15;172(10):5931-9.
ADVANTAGES
12-tissue Northern for human, mouse and rat
Double-purified poly A+ RNA and rigorous QC standards
More RNA than other commercial blots
Everyday low price
Trusted by scientists and proven by numerous citations
Northern Blots eliminate the laborious
need to isolate, separate and transfer
tissue RNA. Quality Northern blots are
obtained by following standard
hybridization conditions and using a
labeled nucleic acid probe.
2 Hybridization
with probe
3 Washing of blot4 Autoradiography/phosphorimaging
1 Pre-hybridization
Product Use
Gen
eex
pre
ssio
nN
orth
ern
Blot
101ph: 1.888.267.4436 fax: 1.301.340.9254
Gen
eex
pre
ssio
nN
orth
ern
Blot
102 www.origene.com
Product Application
A. Human Northern Blots
The human blots are prepared from polyA+ RNAs.
Three different blots covering 22 clinically relevant
tissues are provided.
• Blot I: 12-tissue poly A+ RNA blot (brain, heart,
kidney, lung, small intestine, muscle, spleen, liver,
colon, stomach, testis, placenta)
• Blot II: 12-tissue poly A+ RNA blot (brain,
duodenum, esophagus, pancreas, PBL, prostate,
salivary gland, testis, thymus, thyroid, urinary
bladder, uterus)
• Blot III: 6-tissue poly A+ RNA blot (pancreas,
adrenal gland, ovary, salivary gland, uterus, skin)
OriGene’s pre-made Northern are prepared with
highly enriched polyA+ RNA. The RNA was double
purified by oligo-dT affinity columns. All blots are
normalized by actin expression level. In Fig 1,
HB2010 and HB2011 was hybridized with beta-actin
probe. (Muscle and heart contain shorter alpha actin
that cross-hybridize with beta-actin probe).
As an indication of the quality of the Northern blots,
a human 12-tissue blot I was successively hybridized,
stripped, and re-hybridized to one of three 32P-
labeled, PCR-derived, DNA probes (each about 0.5 kb
in length) (Fig. 2). The three probes used were actin
(panel A), carbonic anhydrase (panel B), and α-
actinin 2 (panel C).
All 12-tissues RNAs contain relatively equal levels of
intact actin mRNA (α-actin in heart and muscle, and
β-actin in the other tissues) (panel A). Low
abundance and tissue-specific transcripts, such as
that of carbonic anhydrase (panel B), are easily
detected. Moreover, closely-related sequences, such
as that of the 4.2-kb α-actinin 2 and 2.9-kb, α-actinin
3 transctipts, can be detected through cross-
hybridization of the probe (panel C). Though less
sensitive than the Rapid-Scan panels, Northern blots
are quantitative and provide information about
transcript sizes and cross-hybridization of mRNA
species.
A
B
C
— 1.8 kb
— 2.4 kb
— 4.2 kb
— 2.9 kb
Fig. 2
Bra
in
Duo
denu
m
Eso
phag
us
Panc
reas
PB
L
Pro
stat
e
Sal
ivar
yG
land
Test
is,T
hym
us
Thy
roid
Uri
nary
Bla
dder
Ute
rus
Bra
in
Lun
g
Hea
rt
Mus
cle
Sto
mac
h
Sm
all
Inte
stin
e
Liv
er
Kid
ney
Spl
een
Test
is
Thy
mus
Ski
n
beta-actin
alpha-actin
Cat#: HB2010 Cat#: HB2011
Fig. 1
Gen
eex
pre
ssio
nN
orth
ern
Blot
103ph: 1.888.267.4436 fax: 1.301.340.9254
B. Mouse Northern Blots
The mouse blots are prepared from poly A+ RNA from
12 major tissues.
• 12-major tissue poly A+ RNA (brain heart,
kidney, liver, lung, muscle, skin, small intestine,
spleen, stomach, testis, thymus)
As an indication of the quality of the Northern blots,
a mouse 12-major tissue blot was successively
hybridized, stripped, and re-hybridized to one of
three 32P-labeled, PCR-derived, DNA probes (each
about 0.5 kb in length), which represent four different
“housekeeping” genes (Fig. 3). The four probes used
were actin (panel A), GAPDH (panel B), a calcium-
binding protein Cab45a (panel C), and phospholipase
A2 (panel D).
The results demonstrate that the blot contains fully
intact RNA and that the actin signal is generally
equal across the blot. Also, it is possible for specific
transcript signals to be effectively normalized
against other “housekeeping” genes in addition to
actin, to which the poly A+ RNA on the blot was
standardized. The relative abundance of a specific
mRNA can be measured against GAPDH,
phospholipase A2, or Cab45a, by simply using the
signal ratio of actin to either of these transcripts and
thus leading to a re-normalization of the signal.
Fig. 3
A
B
C
D
— 1.8 kb
— 1.2 kb
— 2.4 kb
— 5.0 kb
— 2.3 kb
Gen
eex
pre
ssio
nN
orth
ern
Blot
104 www.origene.com
C. Rat Northern Blots
The rat blots are prepared from either poly A+ RNA
from various major tissues or total RNA from
different brain parts.
• 12 major-tissue poly A+ RNA blot (brain,
thymus, lung, heart, muscle, stomach, small
intestine, liver, kidney, spleen, testis, skin)
• 12 brain-part total RNA blot (frontal cortex,
posterior cortex, entorhinal cortex,
hippocampus, olfactory bulb, striatum,
thalamus, cerebellum, midbrain, pons,
medulla, spinal cord)
When the rat 12 major-issue blot was hybridized with
a 32P-labeled actin probe (Fig. 4), the result shows
equal RNA loading in each of the lanes and
intactness of each of the RNAs.
Frontal Cortex
Posterior Cortex
Hippocampus
Olfactory Bulb
Striatum
Thalamus
Cerebellum
Midbrain
Entorhinal Cortex
Medulla
Spinal Cord
Pons
Fig. 4
— 1.8 kb
Gen
eex
pre
ssio
nM
ultip
le-C
hoic
e™cD
NAs
105ph: 1.888.267.4436 fax: 1.301.340.9254
Product Listing Cat. No. Price
Multiple-Choice™ cDNA sets (10 reactions)
Human Multiple-Choice™ First-Strand cDNA Set 1 CH-1101 395(Brain, Heart, Kidney, Spleen, Liver, Peripheral Blood Leukocytes)
Human Multiple-Choice™ First-Strand cDNA Set 2 CH-1102 395(Small Intestine, Muscle, Lung, Prostate, Testis, Ovary)
Human Multiple-Choice™ First-Strand cDNA Set 3 CH-1103 395(Fetal Brain, Fetal Kidney, Fetal Testis, Fetal Liver, Fetal Muscle, Fetal Spleen)
Mouse Multiple-Choice™ First-Strand cDNA Set 1 CM-1101 395(Brain, Heart, Kidney, Spleen, Thymus, Liver)
Mouse Multiple-Choice™ First-Strand cDNA Set 2 CM-1102 395(Stomach, Small Intestine, Muscle, Lung, Testis, Skin)
Rat Multiple-Choice™ First-Strand cDNA Set 1 CR-1101 395(Brain, Heart, Kidney, Spleen, Thymus, Liver)
Rat Multiple-Choice™ First-Strand cDNA Set 2 CR-1102 395(Stomach, Small Intestine, Muscle, Lung, Testis, Skin)
Human First-Strand cDNA (30 reactions)
Human Brain CH-1001 295
Human Heart CH-1002 295
Human Kidney CH-1003 295
Human Liver CH-1005 295
Human Lung CH-1009 295
Human Muscle CH-1008 295
Human Ovary CH-1012 295
Human Peripheral Blood Leukocytes CH-1006 295
Human Placenta CH-1014 295
Multiple-Choice™ cDNAs
Gen
eex
pre
ssio
nM
ultip
le-C
hoic
e™cD
NAs
106 www.origene.com
Product Listing Cat. No. Price
Human Prostate CH-1010 295
Human Small Intestine CH-1007 295
Human Spleen CH-1004 295
Human Stomach CH-1013 295
Human Testis CH-1011 295
Human Fetal Brain CH-1015 295
Human Fetal Kidney CH-1016 295
Human Fetal Liver CH-1018 295
Human Fetal Muscle CH-1019 295
Human Fetal Spleen CH-1020 295
Human Fetal Testis CH-1017 295
Mouse First-Strand cDNA (30 reactions)
Mouse Brain CM-1001 295
Mouse Heart CM-1002 295
Mouse Kidney CM-1003 295
Mouse Liver CM-1006 295
Mouse Lung CM-1010 295
Mouse Muscle CM-1009 295
Mouse Skin CM-1012 295
Mouse Small Intestine CM-1008 295
Mouse Spleen CM-1004 295
Mouse Stomach CM-1007 295
Gen
eex
pre
ssio
nM
ultip
le-C
hoic
e™cD
NAs
107ph: 1.888.267.4436 fax: 1.301.340.9254
Product Listing Cat. No. Price
Mouse Testis CM-1011 295
Mouse Thymus CM-1005 295
Rat First-Strand cDNA (30 reactions)
Rat Brain CR-1001 295
Rat Heart CR-1002 295
Rat Kidney CR-1003 295
Rat Liver CR-1006 295
Rat Lung CR-1010 295
Rat Muscle CR-1009 295
Rat Skin CR-1012 295
Rat Small Intestine CR-1008 295
Rat Spleen CR-1004 295
Rat Stomach CR-1007 295
Rat Testis CR-1011 295
Rat Thymus CR-1005 295
Gen
eex
pre
ssio
nM
ultip
le-C
hoic
e™cD
NAs
108 www.origene.com
Introduction
Amplification of cDNA by PCR is an essential tool for
today’s molecular biologist. This technique can be
used to clone members of a multi-gene family,
homologous genes from different species, and genes
previously identified. PCR amplification of Multiple-
Choice™ First-Strand cDNA and its subsequent
subcloning can often save time and reduce the cost
by eliminating the need to construct and screen a
panel of tissues for the expression of your gene of
interest. Multiple-Choice™ First-Strand cDNA is also
ideal for the characterization of alternatively spliced
mRNA either in a single tissue or across tissues in an
organism.
Product Description
Multiple-Choice™ cDNA is a PCR-ready, first-strand
cDNA prepared from human, mouse or rat. Multiple-
Choice™ First-Strand cDNA is synthesized from
poly A+ RNA, using an oligo(dT) primer and a reverse
transcriptase that favors the production of long
products. It is provided at a concentration of
2-10 ng/µL.
Multiple-Choice™ First-Strand cDNA is available in
two formats:
• Set of six-tissue cDNAs
• Individual tissue cDNA
Product Application
When Multiple-Choice™ cDNA is used as a template
for PCR, it results in specific amplification of the
desired sequence. For example, as shown in Fig. 1,
an actin signal is detectable in each cDNA sample of
the rat Multiple-Choice™ cDNA Set 1, whereas, the
liver-specific cytochrome P450 can only be amplified
from the liver cDNA sample.
ADVANTAGES
Synthesized from high quality poly A+ RNA
Oligo(dT)-primed reverse transcribed
Available as sets of six cDNAs
Control PCR primers included
ActinLiver-Specific
Cytochrome P450
Bra
in
Hea
rt
Kid
ney
Spl
een
Thy
mus
Liv
er
Bra
in
Hea
rt
Kid
ney
Spl
een
Thy
mus
Liv
er
kb
1.6 -
0.5 -
1.0 -
Fig. 1
Gen
eex
pre
ssio
nBl
ue-R
ibbo
n™Po
lyA+
and
tota
lRN
A
109ph: 1.888.267.4436 fax: 1.301.340.9254
Product Listing Cat. No. Price
Human Blue-Ribbon™ RNA
Human Brain, total, 250 µg HT-1001 245
Human Brain, poly A+, 5 µg HM-1001 320
Human Colon, total, 250 µg HT-1015 245
Human Colon, poly A+, 5 µg HM-1015 320
Human Heart, total, 250 µg HT-1002 245
Human Heart, poly A+, 5 µg HM-1002 320
Human Kidney, total, 250 µg HT-1003 245
Human Kidney, poly A+, 5 µg HM-1003 320
Human Liver, total, 250 µg HT-1005 245
Human Liver, poly A+, 5 µg HM-1005 320
Human Lung, total, 250 µg HT-1009 245
Human Lung, poly A+, 5 µg HM-1009 320
Human Muscle, total, 250 µg HT-1008 245
Human Muscle, poly A+, 5 µg HM-1008 320
Human Placenta, total, 250 µg HT-1013 245
Human Placenta, poly A+, 5 µg HM-1013 320
Human Small Intestine, total, 250 µg HT-1007 245
Human Small Intestine, poly A+, 5 µg HM-1007 320
Human Spleen, total, 250 µg HT-1004 245
Human Spleen, poly A+, 5 µg HM-1004 320
Human Stomach, total, 250 µg HT-1014 245
Blue-Ribbon™ Poly A+ and total RNA
Gen
eex
pre
ssio
nBl
ue-R
ibbo
n™Po
lyA+
and
tota
lRN
A
110 www.origene.com
Product Listing Cat. No. Price
Human Stomach, poly A+, 5 µg HM-1014 320
Human Testis, total, 250 µg HT-1011 245
Human Testis, poly A+, 5 µg HM-1011 320
Mouse Blue-Ribbon™ RNA
Mouse Brain, total, 250 µg MT-1001 145
Mouse Brain, poly A+, 5 µg MM-1001 165
Mouse Heart, total, 250 µg MT-1002 145
Mouse Heart, poly A+, 5 µg MM-1002 165
Mouse Kidney, total, 250 µg MT-1003 145
Mouse Kidney, poly A+, 5 µg MM-1003 165
Mouse Liver, total, 250 µg MT-1006 145
Mouse Liver, poly A+, 5 µg MM-1006 165
Mouse Lung, total, 250 µg MT-1010 145
Mouse Lung, poly A+, 5 µg MM-1010 165
Mouse Muscle, total, 250 µg MT-1009 145
Mouse Muscle, poly A+, 5 µg MM-1009 165
Mouse Skin, total, 250 µg MT-1012 145
Mouse Skin, poly A+, 5 µg MM-1012 165
Mouse Small Intestine, total, 250 µg MT-1008 145
Mouse Small Intestine, poly A+, 5 µg MM-1008 165
Mouse Spleen, total, 250 µg MT-1004 145
Mouse Spleen, poly A+, 5 µg MM-1004 165
Mouse Stomach, total, 250 µg MT-1007 145
Gen
eex
pre
ssio
nBl
ue-R
ibbo
n™Po
lyA+
and
tota
lRN
A
111ph: 1.888.267.4436 fax: 1.301.340.9254
Product Listing Cat. No. Price
Mouse Stomach, poly A+, 5 µg MM-1007 165
Mouse Testis, total, 250 µg MT-1011 145
Mouse Testis, poly A+, 5 µg MM-1011 165
Mouse Thymus, total, 250 µg MT-1005 145
Mouse Thymus, poly A+, 5 µg MM-1005 165
Rat Blue-Ribbon™ RNA
Rat Brain, total, 250 µg RT-1001 145
Rat Brain, poly A+, 5 µg RM-1001 165
Rat Heart, total, 250 µg RT-1002 145
Rat Heart, poly A+, 5 µg RM-1002 165
Rat Kidney, total, 250 µg RT-1003 145
Rat Kidney, poly A+, 5 µg RM-1003 165
Rat Liver, total, 250 µg RT-1006 145
Rat Liver, poly A+, 5 µg RM-1006 165
Rat Lung, total, 250 µg RT-1010 145
Rat Lung, poly A+, 5 µg RM-1010 165
Rat Muscle, total, 250 µg RT-1009 145
Rat Muscle, poly A+, 5 µg RM-1009 165
Rat Skin, total, 250 µg RT-1012 145
Rat Skin, poly A+, 5 µg RM-1012 165
Rat Small Intestine, total, 250 µg RT-1008 145
Rat Small Intestine, poly A+, 5 µg RM-1008 165
Rat Spleen, total, 250 µg RT-1004 145
Gen
eex
pre
ssio
nBl
ue-R
ibbo
n™Po
lyA+
and
tota
lRN
A
112 www.origene.com
Product Listing Cat. No. Price
Rat Spleen, poly A+, 5 µg RM-1004 165
Rat Stomach, total, 250 µg RT-1007 145
Rat Stomach, poly A+, 5 µg RM-1007 165
Rat Testis, total, 250 µg RT-1011 145
Rat Testis, poly A+, 5 µg RM-1011 165
Rat Thymus, total, 250 µg RT-1005 145
Rat Thymus, poly A+, 5 µg RM-1005 165
Introduction
RNA preparations from animal tissues can be very
useful in a variety of molecular biology techniques
including Northern blot analysis, RT-PCR, and cDNA
synthesis Blue-Ribbon™ Total and Poly A+ RNA are
available as prepared from either human, mouse, or
rat tissues.
Product Description
Both total RNA or poly A+ RNA samples are available
from 12 major tissues of human, mouse, or rat. Total
RNA is isolated directly from fresh or quick-frozen
tissues to ensure the highest quality products. Total
RNA contains intact 28S and 18S ribosomal RNA
bands as determined by denaturing agarose gel
electrophoresis. Poly A+ RNA is isolated from intact
total RNA using oligo(dT). The isolation method yields
high-quality poly A+ RNA. The RNA is examined by
denaturing agarose gel electrophoresis to ensure that
high molecular weight mRNA is intact. Both total RNA
and poly A+ RNA are supplied in DEPC-treated water.
Quality Control
• All RNA is checked by agarose gel electrophoresis
• Each lot of RNA is Northern blotted and probed
with actin
Blue-Ribbon™ RNA is available in two formats:
• Total RNA (250 µg)
• Poly A+ RNA (5 µg)
ADVANTAGES
High quality total RNA or poly A+ RNA
Choice of 12 major tissues
Human, mouse, or rat
Miscellaneous molecular toolsPre-validated antibodies
Protein collections
Quanti-Ladder™:Broad range DNA size marker (200bp-10kb) with quantity reference
Millennium™ marker:Broad range RNA size marker (0.5kb-9kb)
Rapid-Load™:Innovative PCR compatible loading buffer
Mis
c.m
ole
cula
rto
ols
Pre-
valid
ated
antib
odie
s
114 www.origene.com
Pre-validated Antibodies
Introduction
The OriGene Antibody Collections have been
obtained from suppliers that adhere to the highest
quality standards. Currently OriGene’s antibody
coverage is focused on protein kinases and G protein
coupled receptors (GPCR). These antibodies, pre-
validated for specificity and sensitivity, provide the
convenient and reliable sets of tools for gene
expression and activity monitoring.
Production begins with extensive purification (typ-
ically by both protein A and peptide affinity
chromatography) followed by testing on a wide range
of applications, including ELISA, western blotting,
immunoprecipitation, immunocytochemistry, immu-
nohistochemistry and flow cytometry. For each anti-
body, the QC data is posted on the website for
customer’s evaluation.
All antibodies can be searched by:
• Nucleotide Sequence, Protein Sequence
• Accession or Catalog Number
• Protein Domain
• Keywords
• Gene Family
Product Application
The specificity of the antibody-antigen interaction is
well exploited in laboratory experimentation.
Additionally, the sensitivity of detection for antibody
recognition also drives the variety of applications
listed below. Not all antibodies are qualified for all
applications. Please consult the individual product
specifications.
Western Blots: Proteins from cellular extracts can be
separated through SDS-PAGE and transferred to a
host of solid-surface membranes. Antibodies for the
specific recognition of proteins in limiting amounts
can then be used to probe these membranes.
Immunoprecipitation (IP) or Radioimmunoassay
(RAI):The binding of antibodies to antigens in solution
permits both the purification of antigens (IP) and the
identification / quantification of antigens within a
given sample (RAI).
Immunohistochemistry (IHC) or Immunocytochem-
istry (IC): The molecular characterization of pathology
sections or tissue samples can be addressed by
antibody binding. Antibodies can differentiate cell
types as well as protein expression levels. These are
semi-quantitative approaches that are used to
diagnose disease states or tissue types.
Flow cytometry: Cells presenting specific cell-
surface proteins can be counted via the specific
recognition of antibodies to these antigens. This is
particularly useful in the diagnosis of different cell
types in peripheral blood samples.
Functional studies: Antibodies can be used to block
the function of proteins or other antigens through
steric interference within binding sites or points of
substrate interactions. One can neutralize both
enzyme activity and other types of protein-protein
interactions.
Mis
c.m
ole
cula
rto
ols
Pre-
valid
ated
antib
odie
s
115ph: 1.888.267.4436 fax: 1.301.340.9254
Cat. # Description Format Application Host Sp Cross Reactivity Type Price*
TA201002 BID Antibody (Human Specific) 100 µL (10 WB) W IP Rabbit H Polyclonal 150
TA201003 BID Antibody (Mouse Specific) 100 µL (10 WB) W IP Rabbit M Polyclonal 150
TA201004 BID (7A3) Mouse mAb (Human Specific 100 µL (10 WB) W Mouse H Monoclonal 195
TA201005 BID (7A3) Mouse mAb (Human Specific 300 µL (30 WB) W Mouse H Monoclonal 475
TA201006 IL-1beta Antibody 100 µL (10 WB) W Rabbit H Polyclonal 150
TA201007 Lamin A/C Antibody 100 µL (10 WB) W IHC IC Rabbit H M R Polyclonal 150
TA201008 XIAP Antibody 100 µL (10 WB) W Rabbit H M R Polyclonal 150
TA201009 PKD/PKCmu Antibody 100 µL (10 WB) W Rabbit H M R Mk Polyclonal 150
TA201010 Histone Deacetylase 1 (HDAC1) Antibody 100 µL (10 WB) W IP IHC IC Rabbit H M R Mk Polyclonal 150
TA201011 Histone Deacetylase 4 (HDAC4) Antibody 100 µL (10 WB) W Rabbit H M R Polyclonal 150
TA200491 Neurotensin Receptor Type 1 1 mg/ml IHC Rabbit polyclonal 300
TA200061 G Protein-Coupled Receptor TM7XN1/GPR56 1 mg/ml IHC Rabbit polyclonal 350
TA200085 Melanocortin 4 Receptor (MC4R) 1 mg/ml IHC, ICC Rabbit polyclonal 300
TA201014 Parkin Antibody 100 µL (10 WB) W IP IC Rabbit H M R Polyclonal 150
TA201015 WSTF Antibody 100 µL (10 WB) W IP IC Rabbit H M R Mk Polyclonal 150
TA201016 Histone Deacetylase 6 (HDAC6) Antibody 100 µL (10 WB) W IHC IC Rabbit H M R Mk Polyclonal 150
TA201017 DAP3 Antibody 100 µL (10 WB) W Rabbit H Polyclonal 150
TA201018 DAP5 Antibody 100 µL (10 WB) W Rabbit H M R Polyclonal 150
TA201019 Presenilin 2 Antibody 100 µL (10 WB) W IP IHC IC Rabbit H M R Mk Polyclonal 150
TA201020 Caspase-12 Antibody 100 µL (10 WB) W IC Rabbit M Polyclonal 150
TA201021 S6 Ribosomal Protein Antibody 100 µL (10 WB) W E IHC IC F Rabbit H M R Mk Polyclonal 150
TA201022 S6 Ribosomal Protein (5G10) Rabbit mAb 100 µL (10 WB) W IHC Rabbit H M R Mk Monoclonal 175
TA201023 EGF Receptor Antibody 100 µL (10 WB) W IP IHC IC F Rabbit H M R Polyclonal 150
TA200270 G Protein-Coupled Receptor GPR58 1 mg/ml IHC Rabbit polyclonal 350
TA200105 G Protein-Coupled Receptor GPR31 1 mg/ml IHC Rabbit polyclonal 350
TA200106 G Protein-Coupled Receptor GPR31 1 mg/ml IHC Rabbit polyclonal 250
TA200111 Thyrotropin-Releasing Hormone Receptor (TRH-R) 1 mg/ml IHC Rabbit polyclonal 300
TA201024 EGF Receptor (1F4) Mouse mAb 100 µL (10 WB) W IHC Mouse H Monoclonal 150
TA201025 HER2/ErbB2 Antibody 100 µL (10 WB) W IP IHC Rabbit H M R Polyclonal 150
TA201026 HER2/ErbB2 (44E7) Mouse mAb 100 µL (10 WB) W IC Mouse H Monoclonal 150
TA201027 Skb1Hs Methyltransferase Antibody 100 µL (10 WB) IP W Rabbit H M R Mk Polyclonal 150
TA201028 Myc-Tag (9B11) Mouse mAb 0.2 milligrams W IP IC Rabbit ALL Monoclonal 150
TA201029 DAP1 Antibody 100 µL (10 WB) W IP Rabbit H M R Polyclonal 150
TA201030 DARPP-32 Antibody 100 µL (10 WB) W IHC Rabbit H M R Polyclonal 150
TA201031 Synapsin Antibody 100 µL (10 WB) W Rabbit M R (H) Polyclonal 150
TA201032 eEF2 Antibody 100 µL (10 WB) W IC Rabbit H M R (C) (Hm) Polyclonal 150
TA201033 Dynamin I/II Antibody 100 µL (10 WB) W IP IHC Rabbit H M R Polyclonal 150
TA201034 Chk1 Antibody 100 µL (10 WB) W Rabbit H R Mi Mk Polyclonal 150
TA200112 Glucagon-Like Peptide 1 Receptor 1 mg/ml IHC Rabbit polyclonal 350
TA200113 Glucagon-Like Peptide 1 Receptor 1 mg/ml IHC Rabbit polyclonal 350
TA200263 Prokineticin Receptor 2 (GPR73B) 1 mg/ml IHC Rabbit polyclonal 350
TA201035 His-Tag Polyclonal Antibody 100 µL (10 WB) W IP IC Rabbit ALL Polyclonal 150
TA201036 His-Tag (27E8) Mouse mAb 100 µL (10 WB) W IP IC Mouse ALL Monoclonal 150
TA201037 HA-Tag (6E2) Mouse mAb 100 µL (10 WB) W IP IHC IC F Mouse ALL Monoclonal 150
TA201038 Beta-Gal (14B7) Mouse mAb 0.2 milligrams W IP IC Mouse ALL Monoclonal 150
TA201039 IRS-1 Antibody 100 µL (10 WB) W IP IHC Rabbit H M R Polyclonal 150
Product offering is subject to change. Restricted availability to international customers. Refer to website for the most updated information.
Sp. Cross Reactivity Key: H=Human M=Mouse Mi=Mink Mk=Monkey R=Rat C=Chicken Hm=Hamster X=Xenopus Z=Zebra Fish
Mis
c.m
ole
cula
rto
ols
Pre-
valid
ated
antib
odie
s
116 www.origene.com
Cat. # Description Format Application Host Sp Cross Reactivity Type Price*
TA201040 MBP (8G1) Mouse mAb 0.2 milligrams W IP IC Mouse ALL Monoclonal 150
TA201041 HSP27 (G31) Mouse mAb 100 µL (10 WB) W IHC IC Mouse H Mk Monoclonal 150
TA201042 Shc Antibody 100 µL (10 WB) W Rabbit H M R Polyclonal 150
TA201043 APP Antibody 100 µL (10 WB) W IHC Rabbit H M R Mk Polyclonal 150
TA201044 beta-Amyloid Antibody 100 µL (10 WB) W Rabbit H (R) Polyclonal 150
TA201045 VEGF Receptor 2 Antibody 100 µL (10 WB) W Rabbit H (M) (R) Polyclonal 150
TA201046 PLCbeta3 Antibody 100 µL (10 WB) W IHC IC Rabbit H M R Mk Polyclonal 150
TA200301 Vasopressin V1B Receptor 1 mg/ml IHC Rabbit polyclonal 200
TA200333 G Protein-Coupled Receptor JEG18 1 mg/ml IHC Rabbit polyclonal 350
TA200336 G Protein-Coupled Receptor JEG18 1 mg/ml IHC Rabbit polyclonal 350
TA201047 PPARgamma Antibody 100 µL (10 WB) W IHC Rabbit H M (R) Polyclonal 150
TA201048 Vav Antibody 100 µL (10 WB) W IP IHC Rabbit H R Polyclonal 150
TA201049 Estrogen Receptor alpha (62A3) Mouse mAb 100 µL (10 WB) W Mouse H Monoclonal 150
TA201050 p53 (1C12) Mouse mAb 100 µL (10 WB) W IP IHC IC Mouse H M R Mk Monoclonal 150
TA201051 AMPK-alpha Antibody 100 µL (10 WB) W IP Rabbit H M R Polyclonal 150
TA201052 Paxillin Antibody 100 µL (10 WB) W Rabbit H M R Mk Polyclonal 150
TA201053 p27 Kip1 Antibody 100 µL (10 WB) W IP IHC Rabbit H M R Polyclonal 150
TA201054 Histone H2A Antibody 100 µL (10 WB) W IP IC Rabbit H M R Mk Polyclonal 150
TA201055 PP1alpha Antibody 100 µL (10 WB) W Rabbit H M R Polyclonal 150
TA201056 Histone H4 Antibody 100 µL (10 WB) W IP IHC IC Rabbit H M R Mk Polyclonal 150
TA201057 PAK1 Antibody 100 µL (10 WB) W IP IHC Rabbit H M R Mk Polyclonal 150
TA200351 Glucagon Receptor 1 mg/ml IHC Rabbit polyclonal 250
TA200355 G Protein-Coupled Receptor C5L2 1 mg/ml IHC Rabbit polyclonal 350
TA200356 G Protein-Coupled Receptor C5L2 1 mg/ml IHC Rabbit polyclonal 350
TA201058 PAK1/2/3 Antibody 100 µL (10 WB) W Rabbit H M R Mk Polyclonal 150
TA201059 PAK2 Antibody 100 µL (10 WB) W IP Rabbit H M R Mk Polyclonal 150
TA201060 PAK3 Antibody 100 µL (10 WB) W IP Rabbit H M (R) Polyclonal 150
TA201061 PRK2 Antibody 100 µL (10 WB) W IP Rabbit H M R Polyclonal 150
TA201062 GST Antibody 100 µL (10 WB) W IP IC Rabbit All Polyclonal 150
TA201063 GST (26H1) Mouse mAb 0.2 milligrams W IC Mouse All Monoclonal 150
TA201064 Histone Deacetylase 3 (HDAC3) Antibody 100 µL (10 WB) W IHC IC Rabbit H M R Mk Polyclonal 150
TA201065 Alpha-Synuclein Antibody 100 µL (10 WB) W IP Rabbit H M R Mk Polyclonal 150
TA201066 Chk2 Antibody 100 µL (10 WB) W Rabbit H M R Polyclonal 150
TA201067 IKKalpha Antibody 100 µL (10 WB) W IP IC Rabbit H M R Polyclonal 150
TA201068 IKKbeta Antibody 100 µL (10 WB) W IP Rabbit H M R Polyclonal 150
TA201069 IKKgamma Antibody 100 µL (10 WB) W IHC Rabbit H Mk M R Polyclonal 150
TA201070 IKK epsilon Antibody 100 µL (10 WB) W IP Rabbit H Polyclonal 150
TA201071 Zap-70 (99F2) Rabbit mAb 100 µL (10 WB) W IP IHC F Rabbit H M Monoclonal 150
TA201072 Syk Antibody 100 µL (10 WB) W IC Rabbit H M Polyclonal 150
TA201073 Histone H2B Antibody 100 µL (10 WB) W IP Rabbit H M R Mk Polyclonal 150
TA201074 Lyn Antibody 100 µL (10 WB) W IP IHC Rabbit H M R Polyclonal 150
TA201075 Lck Antibody 100 µL (10 WB) W IP IHC F Rabbit H M Polyclonal 150
TA201076 Bcl-xL Antibody 100 µL (10 WB) W IP IHC Rabbit H M R Mk Polyclonal 150
TA201077 Bax Antibody 100 µL (10 WB) W IP Rabbit H M R Mk Polyclonal 150
TA201078 FADD Antibody (Human Specific) 100 µL (10 WB) W Rabbit H Polyclonal 150
TA201079 Tyrosine Hydroxylase Antibody 100 µL (10 WB) W Rabbit H M R Polyclonal 150
Product offering is subject to change. Restricted availability to international customers. Refer to website for the most updated information.
Sp. Cross Reactivity Key: H=Human M=Mouse Mi=Mink Mk=Monkey R=Rat C=Chicken Hm=Hamster X=Xenopus Z=Zebra Fish
Mis
c.m
ole
cula
rto
ols
Pre-
valid
ated
antib
odie
s
117ph: 1.888.267.4436 fax: 1.301.340.9254
Cat. # Description Format Application Host Sp Cross Reactivity Type Price*
TA201080 Survivin (6E4) Mouse mAb 100 µL (10 WB) W Rabbit H Mk Monoclonal 150
TA201081 MLK3 Antibody 100 µL (10 WB) W Rabbit H M R Hm Mk Polyclonal 150
TA201082 PLCgamma1 Antibody 100 µL (10 WB) W IP Rabbit H M (R) Polyclonal 150
TA201083 cPLA2 Antibody 100 µL (10 WB) W IP Rabbit H M R Polyclonal 150
TA201084 c-Abl Antibody 100 µL (10 WB) W IP IHC F IC Rabbit H M R Polyclonal 150
TA201085 Bcl-2 Antibody 100 µL (10 WB) W IP IHC Rabbit H Polyclonal 150
TA201086 Histone Deacetylase 7 (HDAC7) Antibody 100 µL (10 WB) W IP IC Rabbit H M R Polyclonal 150
TA201087 p18 INK4C (DCS118) Mouse mAb 100 µL (10 WB) W IP Mouse H Monoclonal 150
TA201088 cdk4 (DCS156) Mouse mAb 100 µL (10 WB) W IHC Mouse H M Monoclonal 150
TA201089 cdk7 (MO1) Mouse mAb 100 µL (10 WB) W IP IHC IC Mouse H R Monoclonal 150
TA201090 Cyclin D1 (DCS6) Mouse mAb 100 µL (10 WB) W IP IHC F Mouse H M R Monoclonal 150
TA201091 Cyclin D3 (DCS22) Mouse mAb 100 µL (10 WB) W IHC Mouse H M R Monoclonal 150
TA200001 Beta-2 Adrenoceptor 1 mg/ml IHC, ICC Rabbit polyclonal 300
TA200002 Endothelin B Receptor 1 mg/ml IHC, ICC Rabbit polyclonal 300
TA200003 Bradykinin B2 Receptor 1 mg/ml IHC, ICC Rabbit polyclonal 300
TA200004 Interleukin 8 Receptor A 1 mg/ml IHC, ICC Rabbit polyclonal 300
TA200005 C-C Chemokine Receptor 2 (CCR2) 1 mg/ml IHC Rabbit polyclonal 300
TA200006 Adenosine A2a Receptor 1 mg/ml IHC, ICC Rabbit polyclonal 200
TA200007 Muscarinic Acetylcholine Receptor M3 1 mg/ml IHC Rabbit polyclonal 300
TA200008 Muscarinic Acetylcholine Receptor M3 1 mg/ml IHC Rabbit polyclonal 300
TA200009 Dopamine Receptor D1 1 mg/ml IHC, ICC Rabbit polyclonal 300
TA200010 Dopamine Receptor D1 1 mg/ml IHC, ICC Rabbit polyclonal 300
TA200011 Metabotropic Glutamate Receptor 1 1 mg/ml IHC Rabbit polyclonal 300
TA200012 Metabotropic Glutamate Receptor 2 1 mg/ml IHC, ICC, W Rabbit polyclonal 300
TA200013 Metabotropic Glutamate Receptor 3 1 mg/ml IHC, ICC Rabbit polyclonal 300
TA200014 Metabotropic Glutamate Receptor 3 1 mg/ml IHC, ICC Rabbit polyclonal 300
TA200258 G Protein-Coupled Receptor GPR82 1 mg/ml IHC Rabbit polyclonal 250
TA200189 G Protein-Coupled Receptor GPR32 1 mg/ml IHC Rabbit polyclonal 350
TA200190 G Protein-Coupled Receptor GPR61 1 mg/ml IHC Rabbit polyclonal 350
TA200200 G Protein-Coupled Receptor GPR8 1 mg/ml IHC Rabbit polyclonal 350
TA200015 Metabotropic Glutamate Receptor 3 1 mg/ml IHC, ICC Rabbit polyclonal 300
TA200016 Metabotropic Glutamate Receptor 8 1 mg/ml IHC Rabbit polyclonal 300
TA200017 Metabotropic Glutamate Receptor 8 1 mg/ml IHC Rabbit polyclonal 300
TA200018 5-HT4 Receptor 1 mg/ml IHC Rabbit polyclonal 250
TA200020 Prostaglandin E Receptor EP2 1 mg/ml IHC Rabbit polyclonal 300
TA200114 G Protein-Coupled Receptor GPR3 1 mg/ml IHC Rabbit polyclonal 350
TA200115 Retinoic Acid-Induced 3 (RAI3/RAIG1) 1 mg/ml IHC, ICC Rabbit polyclonal 250
TA200116 Platelet Activating Receptor Homolog (H963) 1 mg/ml IHC Rabbit polyclonal 350
TA200117 Platelet Activating Receptor Homolog (H963) 1 mg/ml IHC Rabbit polyclonal 350
TA200118 Alpha 1c-Adrenoceptor 1 mg/ml IHC Rabbit polyclonal 300
TA200119 G Protein-Coupled Receptor GPR35 1 mg/ml IHC Rabbit polyclonal 350
TA200120 Gastric Inhibitory Polypeptide Receptor 1 mg/ml IHC Rabbit polyclonal 250
TA200121 Gastric Inhibitory Polypeptide Receptor 1 mg/ml IHC Rabbit polyclonal 250
TA200122 Gastric Inhibitory Polypeptide Receptor 1 mg/ml IHC Rabbit polyclonal 350
TA200123 G Protein-Coupled Receptor GPR34 1 mg/ml IHC Rabbit polyclonal 350
TA200202 G Protein-Coupled Receptor SLT/MCH2 1 mg/ml IHC, ICC Rabbit polyclonal 200
Product offering is subject to change. Restricted availability to international customers. Refer to website for the most updated information.
Sp. Cross Reactivity Key: H=Human M=Mouse Mi=Mink Mk=Monkey R=Rat C=Chicken Hm=Hamster X=Xenopus Z=Zebra Fish
Mis
c.m
ole
cula
rto
ols
Pre-
valid
ated
antib
odie
s
118 www.origene.com
Cat. # Description Format Application Host Sp Cross Reactivity Type Price*
TA200203 G Protein-Coupled Receptor SLT/MCH2 1 mg/ml IHC Rabbit polyclonal 300
TA200205 Lysophosphatidic Acid Receptor 1 mg/ml IHC Rabbit polyclonal 350
P2Y9/LPA4/GPR23
TA200207 G Protein-Coupled Receptor SLT/MCH2 1 mg/ml IHC, ICC Rabbit polyclonal 300
TA200124 Neurotensin Receptor Type 2 (NTR2) 1 mg/ml IHC Rabbit polyclonal 300
TA200125 G Protein-Coupled Receptor GPR45 1 mg/ml IHC, ICC Rabbit polyclonal 350
TA200126 Thromboxane A2 Receptor (TBXA2R) 1 mg/ml IHC Rabbit polyclonal 250
TA200127 Thromboxane A2 Receptor (TBXA2R) 1 mg/ml IHC Rabbit polyclonal 250
TA200128 Tachykinin Receptor 2 1 mg/ml IHC, ICC Rabbit polyclonal 350
TA200129 PAEL Receptor (GPR37) 1 mg/ml IHC Rabbit polyclonal 300
TA200130 Tachykinin Receptor 2 1 mg/ml ICC Rabbit polyclonal 250
TA200133 Vasoactive Intestinal Polypeptide Receptor 2 1 mg/ml IHC Rabbit polyclonal 300
TA200134 Vasoactive Intestinal Polypeptide Receptor 2 1 mg/ml IHC Rabbit polyclonal 300
TA200131 Vasoactive Intestinal Polypeptide Receptor 1 1 mg/ml IHC Rabbit polyclonal 200
TA200132 Vasoactive Intestinal Polypeptide Receptor 1 1 mg/ml IHC Rabbit polyclonal 300
TA200135 Proteinase-Activated Receptor 4 1 mg/ml IHC Rabbit polyclonal 200
TA200136 Proteinase-Activated Receptor 4 1 mg/ml IHC Rabbit polyclonal 300
TA200137 Proteinase-Activated Receptor 4 1 mg/ml IHC Rabbit polyclonal 300
TA200138 Proteinase-Activated Receptor 4 1 mg/ml IHC Rabbit polyclonal 200
TA200139 Glucagon-Like Peptide 2 Receptor 1 mg/ml IHC Rabbit polyclonal 350
TA200140 Glucagon-Like Peptide 2 Receptor 1 mg/ml IHC Rabbit polyclonal 350
TA200208 Purinergic Receptor P2Y8 1 mg/ml IHC Rabbit polyclonal 250
TA200209 Purinergic Receptor P2Y8 1 mg/ml IHC Rabbit polyclonal 300
TA200210 G Protein-Coupled Receptor GPR103 1 mg/ml IHC Rabbit polyclonal 350
TA200141 Cysteinyl Leukotriene Receptor 1 (CysLT1) 1 mg/ml IHC Rabbit polyclonal 300
TA200142 Cysteinyl Leukotriene Receptor 1 (CysLT1) 1 mg/ml IHC Rabbit polyclonal 300
TA200143 Muscarinic Acetylcholine Receptor M2 1 mg/ml IHC Rabbit polyclonal 200
TA200144 Muscarinic Acetylcholine Receptor M2 1 mg/ml IHC Rabbit polyclonal 300
TA200145 Muscarinic Acetylcholine Receptor M2 1 mg/ml IHC Rabbit polyclonal 300
TA200146 Muscarinic Acetylcholine Receptor M5 1 mg/ml IHC Rabbit polyclonal 300
TA200147 Muscarinic Acetylcholine Receptor M5 1 mg/ml IHC Rabbit polyclonal 300
TA200148 Tachykinin Receptor 1 1 mg/ml IHC Rabbit polyclonal 300
TA200149 CXC Chemokine Receptor 3 (CXCR3) 1 mg/ml IHC Rabbit polyclonal 300
TA200173 G Protein-Coupled Receptor GPR87/GPR95 1 mg/ml IHC Rabbit polyclonal 300
TA200174 G Protein-Coupled Receptor GPR87/GPR95 1 mg/ml IHC Rabbit polyclonal 200
TA200175 G Protein-Coupled Receptor GPR75 1 mg/ml IHC, ICC Rabbit polyclonal 200
TA200150 CXC Chemokine Receptor 5 (CXCR5) 1 mg/ml IHC Rabbit polyclonal 300
TA200151 CXC Chemokine Receptor 5 (CXCR5) 1 mg/ml IHC Rabbit polyclonal 300
TA200152 C-C Chemokine Receptor 3 (CCR3) 1 mg/ml IHC Rabbit polyclonal 300
TA200374 Bombesin Receptor Subtype 3 (BRS3) 1 mg/ml IHC Rabbit polyclonal 300
TA200375 Frizzled-9 (FZD9) 1 mg/ml IHC Rabbit polyclonal 350
TA200376 Frizzled-9 (FZD9) 1 mg/ml IHC Rabbit polyclonal 350
TA200153 C-C Chemokine Receptor 3 (CCR3) 1 mg/ml IHC Rabbit polyclonal 300
TA200154 G Protein-Coupled Receptor GPR39 1 mg/ml IHC Rabbit polyclonal 350
TA200155 G Protein-Coupled Receptor GPR39 1 mg/ml IHC Rabbit polyclonal 350
TA200156 G Protein-Coupled Receptor RDC1 1 mg/ml IHC Rabbit polyclonal 350
Product offering is subject to change. Restricted availability to international customers. Refer to website for the most updated information.
Sp. Cross Reactivity Key: H=Human M=Mouse Mi=Mink Mk=Monkey R=Rat C=Chicken Hm=Hamster X=Xenopus Z=Zebra Fish
Mis
c.m
ole
cula
rto
ols
Pre-
valid
ated
antib
odie
s
119ph: 1.888.267.4436 fax: 1.301.340.9254
Cat. # Description Format Application Host Sp Cross Reactivity Type Price*
TA200157 Chemokine (C-C motif) Receptor-Like 1 (CCRL1) 1 mg/ml IHC Rabbit polyclonal 300
TA200158 Chemokine (C-C motif) Receptor-Like 1 (CCRL1) 1 mg/ml IHC Rabbit polyclonal 200
TA200159 Thyrotropin (TSH) Receptor 1 mg/ml IHC Rabbit polyclonal 300
TA200160 Thyrotropin (TSH) Receptor 1 mg/ml IHC Rabbit polyclonal 300
TA200161 Thyrotropin (TSH) Receptor 1 mg/ml IHC Rabbit polyclonal 200
TA200162 G Protein-Coupled Receptor GPR105 1 mg/ml IHC, ICC Rabbit polyclonal 350
TA200163 Latrophilin-3 1 mg/ml IHC Rabbit polyclonal 250
TA200164 G Protein-Coupled Receptor LOC51210 1 mg/ml IHC Rabbit polyclonal 350
TA200168 G Protein-Coupled Receptor SLC/MCH1 1 mg/ml IHC Rabbit polyclonal 200
TA200169 G Protein-Coupled Receptor SLC/MCH1 1 mg/ml IHC Rabbit polyclonal 300
TA200171 Chemokine (C motif) XC Receptor 1 (CCXCR1) 1 mg/ml IHC Rabbit polyclonal 300
TA200172 G Protein-Coupled Receptor GPR43 1 mg/ml IHC Rabbit polyclonal 250
TA200165 G Protein-Coupled Receptor LOC51210 1 mg/ml IHC, ICC Rabbit polyclonal 350
TA200166 Mas Proto-Oncogene 1 mg/ml IHC Rabbit polyclonal 350
TA200167 Mas Proto-Oncogene 1 mg/ml IHC Rabbit polyclonal 350
TA200170 P2Y12 Platelet ADP Receptor 1 mg/ml IHC, ICC Rabbit polyclonal 350
TA200176 G Protein-Coupled Receptor GPR75 1 mg/ml IHC, ICC Rabbit polyclonal 200
TA200177 G Protein-Coupled Receptor GPR40 1 mg/ml IHC Rabbit polyclonal 350
TA200178 G Protein-Coupled Receptor GPR40 1 mg/ml IHC Rabbit polyclonal 350
TA200179 G Protein-Coupled Receptor G2A 1 mg/ml IHC Rabbit polyclonal 350
TA200180 G Protein-Coupled Receptor GPR72 1 mg/ml IHC Rabbit polyclonal 250
TA200181 G Protein-Coupled Receptor GPR72 1 mg/ml IHC Rabbit polyclonal 350
TA200182 G Protein-Coupled Receptor GPR15 1 mg/ml IHC Rabbit polyclonal 300
TA200183 G Protein-Coupled Receptor GPR86/GPR94/P2Y13 1 mg/ml IHC Rabbit polyclonal 250
TA200184 T-Cell Death-Associated Gene 8 (GPR65) 1 mg/ml IHC Rabbit polyclonal 300
TA200185 T-Cell Death-Associated Gene 8 (GPR65) 1 mg/ml IHC Rabbit polyclonal 300
TA200186 G Protein-Coupled Receptor GPR48 1 mg/ml IHC Rabbit polyclonal 250
TA200187 EGF-Like Module-Containing (EMR2) 1 mg/ml IHC, ICC Rabbit polyclonal 250
Mucin-Like Receptor 2
TA200254 Xenotropic And Polytropic 1 mg/ml IHC, ICC Rabbit polyclonal 200
Retrovirus Receptor (XPR1)
TA200256 G Protein-Coupled Receptor GPR82 1 mg/ml IHC Rabbit polyclonal 350
TA200257 G Protein-Coupled Receptor GPR82 1 mg/ml IHC Rabbit polyclonal 350
TA200188 G Protein-Coupled Receptor GPR6 1 mg/ml IHC Rabbit polyclonal 350
TA200191 G Protein-Coupled Receptor GPR6 1 mg/ml IHC Rabbit polyclonal 350
TA200192 Protein A 1 mg/ml IHC Rabbit polyclonal 350
TA200193 Protein A 1 mg/ml IHC Rabbit polyclonal 350
TA200194 Protein A 1 mg/ml IHC Rabbit polyclonal 250
TA200195 G Protein-Coupled Receptor GPR7 1 mg/ml IHC Rabbit polyclonal 350
TA200196 G Protein-Coupled Receptor GPR7 1 mg/ml IHC Rabbit polyclonal 350
TA200197 G Protein-Coupled Receptor GPR63 (PSP24 Beta) 1 mg/ml IHC Rabbit polyclonal 350
TA200198 G Protein-Coupled Receptor SALPR/GPCR135 1 mg/ml IHC Rabbit polyclonal 350
TA200199 G Protein-Coupled Receptor SALPR/GPCR135 1 mg/ml IHC Rabbit polyclonal 350
TA200201 Muscarinic Acetylcholine Receptor M2 1 mg/ml IHC Rabbit polyclonal 200
TA200204 G Protein-Coupled Receptor RDC1 1 mg/ml IHC Rabbit polyclonal 350
TA200206 C-C Chemokine Receptor 2 (CCR2) 1 mg/ml IHC Rabbit polyclonal 200
Product offering is subject to change. Restricted availability to international customers. Refer to website for the most updated information.
Sp. Cross Reactivity Key: H=Human M=Mouse Mi=Mink Mk=Monkey R=Rat C=Chicken Hm=Hamster X=Xenopus Z=Zebra Fish
Mis
c.m
ole
cula
rto
ols
Pre-
valid
ated
antib
odie
s
120 www.origene.com
Cat. # Description Format Application Host Sp Cross Reactivity Type Price*
TA200211 G Protein-Coupled Receptor GPR54 1 mg/ml IHC Rabbit polyclonal 250
TA200212 G Protein-Coupled Receptor GPR54 1 mg/ml IHC Rabbit polyclonal 350
TA200213 G Protein-Coupled Receptor GPR54 1 mg/ml IHC Rabbit polyclonal 250
TA200214 G Protein-Coupled Receptor GPR54 1 mg/ml IHC Rabbit polyclonal 350
TA200215 G Protein-Coupled Receptor GPR146 1 mg/ml IHC Rabbit polyclonal 350
TA200216 G Protein-Coupled Receptor GPR35 1 mg/ml IHC, ICC Rabbit polyclonal 350
TA200217 Prostate-Specific G Protein-Coupled 1 mg/ml IHC Rabbit polyclonal 350
Receptor (PSGR)
TA200218 Galanin Receptor GalR3 1 mg/ml IHC Rabbit polyclonal 300
TA200219 Galanin Receptor GalR3 1 mg/ml IHC Rabbit polyclonal 300
TA200220 Formyl Peptide Receptor 1 (FPR1) 1 mg/ml IHC Rabbit polyclonal 200
TA200221 Gonadotropin-Releasing Hormone Receptor 1 mg/ml IHC Rabbit polyclonal 300
TA200222 Chemokine Receptor FKSG80/GPR81 1 mg/ml IHC Rabbit polyclonal 350
TA200223 Chemokine Receptor FKSG80/GPR81 1 mg/ml IHC Rabbit polyclonal 350
TA200248 Somatostatin Receptor Type 3 1 mg/ml IHC Rabbit polyclonal 350
TA200249 Somatostatin Receptor Type 3 1 mg/ml IHC Rabbit polyclonal 350
TA200251 Vasopressin V1B Receptor 1 mg/ml IHC Rabbit polyclonal 300
TA200253 Xenotropic And Polytropic 1 mg/ml IHC, ICC Rabbit polyclonal 300
Retrovirus Receptor (XPR1)
TA200231 Melatonin Receptor Type 1A (MT1) 1 mg/ml IHC Rabbit polyclonal 350
TA200232 Melatonin Receptor Type 1A (MT1) 1 mg/ml IHC Rabbit polyclonal 350
TA200235 Follicle-Stimulating Hormone Receptor (FSHR) 1 mg/ml IHC Rabbit polyclonal 200
TA200224 Leukotriene B4 Receptor BLT2 1 mg/ml IHC Rabbit polyclonal 200
TA200225 Leukotriene B4 Receptor BLT2 1 mg/ml IHC Rabbit polyclonal 300
TA200226 Leukotriene B4 Receptor BLT2 1 mg/ml IHC Rabbit polyclonal 300
TA200227 Lysophosphatidic Acid Receptor Edg2/LPA1 1 mg/ml IHC Rabbit polyclonal 300
TA200228 Lysophosphatidic Acid Receptor Edg2/LPA1 1 mg/ml IHC Rabbit polyclonal 300
TA200229 Encephalopsin 1 mg/ml IHC Rabbit polyclonal 350
TA200230 Encephalopsin 1 mg/ml IHC Rabbit polyclonal 350
TA200233 Muscarinic Acetylcholine Receptor M4 1 mg/ml IHC Rabbit polyclonal 200
TA200234 Muscarinic Acetylcholine Receptor M4 1 mg/ml IHC Rabbit polyclonal 200
TA200236 G Protein-Coupled Receptor TG1019 1 mg/ml IHC Rabbit polyclonal 350
TA200237 Opioid Receptor, Mu 1 (OPRM1) 1 mg/ml IHC Rabbit polyclonal 300
TA200238 Retinoid-Related Orphan Receptor 1 mg/ml IHC Rabbit polyclonal 250
Alpha (ROR Alpha/NR1F1)
TA200239 Opioid Receptor, Mu 1 (OPRM1) 1 mg/ml IHC Rabbit polyclonal 300
TA200243 Proteinase-Activated Receptor 2 1 mg/ml IHC Rabbit polyclonal 300
TA200244 Proteinase-Activated Receptor 2 1 mg/ml IHC Rabbit polyclonal 300
TA200062 G Protein-Coupled Receptor TM7XN1/GPR56 1 mg/ml IHC Rabbit polyclonal 350
TA200063 G Protein-Coupled Receptor GPR64 (HE6) 1 mg/ml IHC Rabbit polyclonal 350
TA200064 G Protein-Coupled Receptor GPR64 (HE6) 1 mg/ml IHC Rabbit polyclonal 350
TA200246 Alpha1-Fetoprotein Transcription Factor (NR5A2) 1 mg/ml IHC Rabbit polyclonal 250
TA200247 Xenotropic And Polytropic 1 mg/ml IHC Rabbit polyclonal 200
Retrovirus Receptor (XPR1)
TA200019 Neuropeptide Y Receptor Type 1 1 mg/ml IHC Rabbit polyclonal 300
TA200021 Prostaglandin E2 Receptor EP3 1 mg/ml IHC Rabbit polyclonal 300
TA200022 Prostaglandin E Receptor EP4 1 mg/ml IHC Rabbit polyclonal 300
Product offering is subject to change. Restricted availability to international customers. Refer to website for the most updated information.
Sp. Cross Reactivity Key: H=Human M=Mouse Mi=Mink Mk=Monkey R=Rat C=Chicken Hm=Hamster X=Xenopus Z=Zebra Fish
Mis
c.m
ole
cula
rto
ols
Pre-
valid
ated
antib
odie
s
121ph: 1.888.267.4436 fax: 1.301.340.9254
Cat. # Description Format Application Host Sp Cross Reactivity Type Price*
TA200023 Prostaglandin F2-Alpha Receptor 1 mg/ml IHC, ICC Rabbit polyclonal 350
TA200024 Prostaglandin F2-Alpha Receptor 1 mg/ml IHC Rabbit polyclonal 350
TA200025 C-C Chemokine Receptor 1 (CCR1) 1 mg/ml IHC Rabbit polyclonal 300
TA200026 Sphingolipid Receptor Edg1/S1P1 1 mg/ml IHC Rabbit polyclonal 300
TA200027 Sphingolipid Receptor Edg1/S1P1 1 mg/ml IHC Rabbit polyclonal 300
TA200028 Estrogen-Related Receptor 1 mg/ml IHC Rabbit polyclonal 350
Gamma (ERR Gamma/NR3B3)
TA200029 Estrogen-Related Receptor 1 mg/ml IHC Rabbit polyclonal 350
Gamma (ERR Gamma/NR3B3)
TA200030 Formyl Peptide Receptor-Like Receptor 1 (FPRL1) 1 mg/ml IHC Rabbit polyclonal 300
TA200031 GABA(B) Receptor 1 1 mg/ml IHC Rabbit polyclonal 300
TA200032 GABA(B) Receptor 1 1 mg/ml IHC Rabbit polyclonal 300
TA200050 Proteinase-Activated Receptor 3 1 mg/ml IHC Rabbit polyclonal 300
TA200058 Sphingolipid Receptor Edg3/S1P3 1 mg/ml IHC, ICC Rabbit polyclonal 300
TA200033 G Protein-Coupled Receptor GPR30 1 mg/ml IHC Rabbit polyclonal 350
TA200034 Brain-Specific Angiogenesis Inhibitor 3 (BAI3) 1 mg/ml IHC Rabbit polyclonal 350
TA200057 Parathyroid Hormone Receptor 2 (PTHR2) 1 mg/ml IHC Rabbit polyclonal 300
TA200035 Brain-Specific Angiogenesis Inhibitor 3 (BAI3) 1 mg/ml IHC Rabbit polyclonal 350
TA200036 C-C Chemokine Receptor 7 (CCR7) 1 mg/ml IHC Rabbit polyclonal 300
TA200037 Cannabinoid Receptor 1 (CB1) 1 mg/ml IHC, ICC Rabbit polyclonal 300
TA200038 Cannabinoid Receptor 1 (CB1) 1 mg/ml IHC, ICC Rabbit polyclonal 300
TA200039 Endothelin A Receptor 1 mg/ml IHC Rabbit polyclonal 300
TA200040 Thrombin Receptor 1 mg/ml IHC Rabbit polyclonal 300
TA200041 Formyl Peptide Receptor 1 (FPR1) 1 mg/ml IHC Rabbit polyclonal 300
TA200042 Formyl Peptide Receptor-Like 2 (FPRL2) 1 mg/ml IHC Rabbit polyclonal 350
TA200043 Formyl Peptide Receptor-Like 2 (FPRL2) 1 mg/ml IHC, ICC Rabbit polyclonal 350
TA200044 CXC Chemokine Receptor 4 (CXCR4) 1 mg/ml IHC, ICC Rabbit polyclonal 300
TA200045 CXC Chemokine Receptor 4 (CXCR4) 1 mg/ml IHC Rabbit polyclonal 300
TA200272 Purinergic Receptor P2U2 (GPR91) 1 mg/ml IHC Rabbit polyclonal 350
TA200371 G Protein-Coupled Receptor GPR72 1 mg/ml IHC Rabbit polyclonal 350
TA200373 Pyrimidinergic Receptor P2Y4 1 mg/ml IHC Rabbit polyclonal 350
TA200046 G Protein-Coupled Receptor GPR49 1 mg/ml IHC Rabbit polyclonal 350
TA200047 G Protein-Coupled Receptor GPR49 1 mg/ml IHC Rabbit polyclonal 350
TA200048 Chemokine-Like Receptor 1 (CMKLR1) 1 mg/ml IHC Rabbit polyclonal 350
TA200110 G Protein-Coupled Receptor GPR3 1 mg/ml IHC Rabbit polyclonal 350
TA200049 Chemokine-Like Receptor 1 (CMKLR1) 1 mg/ml IHC Rabbit polyclonal 350
TA200051 Purinergic Receptor P2Y6 1 mg/ml IHC Rabbit polyclonal 350
TA200052 Purinergic Receptor P2Y6 1 mg/ml IHC, ICC Rabbit polyclonal 350
TA200053 Vasoactive Intestinal Polypeptide Receptor 1 1 mg/ml IHC Rabbit polyclonal 300
TA200054 Lysophosphatidic Acid Receptor Edg4/LPA2 1 mg/ml IHC, ICC Rabbit polyclonal 300
TA200055 G Protein-Coupled Receptor GPR44 (CRTH2) 1 mg/ml IHC, ICC Rabbit polyclonal 350
TA200056 Parathyroid Hormone Receptor 2 (PTHR2) 1 mg/ml IHC Rabbit polyclonal 300
TA200059 G Protein-Coupled Receptor GPR4 1 mg/ml IHC Rabbit polyclonal 350
TA200060 G Protein-Coupled Receptor GPR4 1 mg/ml IHC, ICC Rabbit polyclonal 350
TA200065 Calcitonin Receptor-Like Receptor 1 mg/ml IHC, ICC Rabbit polyclonal 350
TA200265 Prokineticin Receptor 2 (GPR73B) 1 mg/ml IHC Rabbit polyclonal 350
Product offering is subject to change. Restricted availability to international customers. Refer to website for the most updated information.
Sp. Cross Reactivity Key: H=Human M=Mouse Mi=Mink Mk=Monkey R=Rat C=Chicken Hm=Hamster X=Xenopus Z=Zebra Fish
Mis
c.m
ole
cula
rto
ols
Pre-
valid
ated
antib
odie
s
122 www.origene.com
Cat. # Description Format Application Host Sp Cross Reactivity Type Price*
TA200266 Putative Neurotransmitter Receptor (PNR) 1 mg/ml IHC Rabbit polyclonal 350
TA200337 G Protein-Coupled Receptor GPR26 1 mg/ml IHC Rabbit polyclonal 300
TA200066 Calcitonin Receptor-Like Receptor 1 mg/ml IHC, ICC Rabbit polyclonal 350
TA200067 G Protein-Coupled Receptor HM74 1 mg/ml IHC, ICC Rabbit polyclonal 350
TA200068 G Protein-Coupled Receptor HM74 1 mg/ml IHC, ICC Rabbit polyclonal 350
TA200069 G Protein-Coupled Receptor HM74 1 mg/ml IHC Rabbit polyclonal 350
TA200070 Frizzled-10 (FZD10) 1 mg/ml IHC Rabbit polyclonal 350
TA200073 G Protein-Coupled Receptor RE2 1 mg/ml IHC, ICC Rabbit polyclonal 350
TA200075 G Protein-Coupled Receptor GPR48 1 mg/ml IHC Rabbit polyclonal 350
TA200076 G Protein-Coupled Receptor GPR86/GPR94/P2Y13 1 mg/ml IHC Rabbit polyclonal 350
TA200071 G Protein-Coupled Receptor AGR9 1 mg/ml IHC Rabbit polyclonal 350
TA200072 G Protein-Coupled Receptor AGR9 1 mg/ml IHC Rabbit polyclonal 350
TA200074 G Protein-Coupled Receptor GPR48 1 mg/ml IHC Rabbit polyclonal 350
TA200077 G Protein-Coupled Receptor GPR86/GPR94/P2Y13 1 mg/ml IHC Rabbit polyclonal 350
TA200078 Adenosine A1 Receptor 1 mg/ml IHC Rabbit polyclonal 300
TA200079 G Protein-Coupled Receptor GPR20 1 mg/ml IHC Rabbit polyclonal 350
TA200080 Lysophosphatidic Acid Receptor Edg7/LPA3 1 mg/ml IHC Rabbit polyclonal 300
TA200082 Sphingolipid Receptor Edg5/S1P2 1 mg/ml IHC, ICC Rabbit polyclonal 300
TA200083 Sphingolipid Receptor Edg5/S1P2 1 mg/ml IHC, ICC Rabbit polyclonal 300
TA200084 Sphingolipid Receptor Edg5/S1P2 1 mg/ml IHC, ICC Rabbit polyclonal 200
TA200081 Lysophosphatidic Acid Receptor Edg7/LPA3 1 mg/ml IHC Rabbit polyclonal 300
TA200086 Melanocortin 1 Receptor (MC1R) 1 mg/ml IHC Rabbit polyclonal 300
TA200087 Melanocortin 1 Receptor (MC1R) 1 mg/ml IHC Rabbit polyclonal 200
TA200088 Prostaglandin F2-Alpha Receptor 1 mg/ml IHC Rabbit polyclonal 250
TA200089 Neuropeptide Y Receptor Type 4 1 mg/ml IHC Rabbit polyclonal 350
TA200090 Neuropeptide Y Receptor Type 4 1 mg/ml IHC Rabbit polyclonal 250
TA200099 Latrophilin-2 1 mg/ml IHC Rabbit polyclonal 350
TA200100 Latrophilin-2 1 mg/ml IHC Rabbit polyclonal 250
TA200101 Latrophilin-2 1 mg/ml IHC Rabbit polyclonal 350
TA200091 Neuropeptide Y Receptor Type 4 1 mg/ml IHC Rabbit polyclonal 350
TA200092 Neuropeptide Y Receptor Type 5 1 mg/ml IHC, ICC Rabbit polyclonal 250
TA200093 Neuropeptide Y Receptor Type 5 1 mg/ml IHC, ICC Rabbit polyclonal 350
TA200094 Neuropeptide Y Receptor Type 5 1 mg/ml IHC, ICC Rabbit polyclonal 250
TA200095 Prolactin Releasing Hormone Receptor (GPR10) 1 mg/ml IHC Rabbit polyclonal 250
TA200096 Chemokine (C-C motif) Receptor-like 2 (CCRL2) 1 mg/ml IHC Rabbit polyclonal 350
TA200267 Putative Neurotransmitter Receptor (PNR) 1 mg/ml IHC Rabbit polyclonal 350
TA200268 G Protein-Coupled Receptor GPR75 1 mg/ml IHC, ICC Rabbit polyclonal 200
TA200269 G Protein-Coupled Receptor GPR75 1 mg/ml IHC, ICC Rabbit polyclonal 200
TA200097 Chemokine (C-C motif) Receptor-like 2 (CCRL2) 1 mg/ml IHC Rabbit polyclonal 350
TA200098 G Protein-Coupled Receptor GPR22 1 mg/ml IHC Rabbit polyclonal 350
TA200102 Latrophilin-3 1 mg/ml IHC Rabbit polyclonal 350
TA200103 G Protein-Coupled Receptor GPR2/CCR10 1 mg/ml IHC Rabbit polyclonal 300
TA200104 G Protein-Coupled Receptor GPR2/CCR10 1 mg/ml IHC Rabbit polyclonal 300
TA200107 Histamine H1 Receptor 1 mg/ml IHC Rabbit polyclonal 300
TA200108 G Protein-Coupled Receptor GPR30 1 mg/ml IHC Rabbit polyclonal 350
TA200109 Alpha 1d-Adrenoceptor 1 mg/ml IHC Rabbit polyclonal 300
Product offering is subject to change. Restricted availability to international customers. Refer to website for the most updated information.
Sp. Cross Reactivity Key: H=Human M=Mouse Mi=Mink Mk=Monkey R=Rat C=Chicken Hm=Hamster X=Xenopus Z=Zebra Fish
Mis
c.m
ole
cula
rto
ols
Pre-
valid
ated
antib
odie
s
123ph: 1.888.267.4436 fax: 1.301.340.9254
Cat. # Description Format Application Host Sp Cross Reactivity Type Price*
TA200240 Retinoid-Related Orphan 1 mg/ml IHC Rabbit polyclonal 350
Receptor Beta (ROR Beta/NR1F2)
TA200241 Oxytocin Receptor 1 mg/ml IHC Rabbit polyclonal 300
TA200242 Oxytocin Receptor 1 mg/ml IHC Rabbit polyclonal 300
TA200245 Retinoid-Related Orphan 1 mg/ml IHC Rabbit polyclonal 350
Receptor Beta (ROR Beta/NR1F2)
TA200250 Somatostatin Receptor Type 5 (SSTR5) 1 mg/ml IHC Rabbit polyclonal 300
TA200252 Beta-2 Adrenoceptor 1 mg/ml IHC Rabbit polyclonal 250
TA200255 Metabotropic Glutamate Receptor 7 1 mg/ml IHC Rabbit polyclonal 250
TA200259 Vasopressin V2 Receptor 1 mg/ml IHC Rabbit polyclonal 300
TA200260 Vasopressin V2 Receptor 1 mg/ml IHC Rabbit polyclonal 300
TA200261 G Protein-Coupled Receptor MRGX2 1 mg/ml IHC Rabbit polyclonal 350
TA200262 G Protein-Coupled Receptor GPR35 1 mg/ml IHC, ICC Rabbit polyclonal 350
TA200264 Prokineticin Receptor 1 (GPR73A) 1 mg/ml IHC Rabbit polyclonal 300
TA200271 Cysteinyl Leukotriene Receptor 2 (CysLT2) 1 mg/ml IHC Rabbit polyclonal 250
TA200273 5-HT1F Receptor 1 mg/ml IHC Rabbit polyclonal 200
TA200274 5-HT1F Receptor 1 mg/ml IHC Rabbit polyclonal 200
TA200275 5-HT1F Receptor 1 mg/ml IHC Rabbit polyclonal 200
TA200276 G Protein-Coupled Receptor GPR85 (SREB2) 1 mg/ml IHC Rabbit polyclonal 350
TA200277 G Protein-Coupled Receptor GPR85 (SREB2) 1 mg/ml IHC Rabbit polyclonal 350
TA200278 G Protein-Coupled Receptor EX33 1 mg/ml IHC Rabbit polyclonal 350
TA200279 G Protein-Coupled Receptor EX33 1 mg/ml IHC Rabbit polyclonal 350
TA200280 Vomeronasal 1 Receptor 1 (VN1R1) 1 mg/ml IHC Rabbit polyclonal 250
TA200283 Vomeronasal 1 Receptor 1 (VN1R1) 1 mg/ml IHC Rabbit polyclonal 250
TA200284 G Protein-Coupled Receptor GPR52 1 mg/ml IHC Rabbit polyclonal 350
TA200291 G Protein-Coupled Receptor GPR48 1 mg/ml IHC Rabbit polyclonal 250
TA200281 Vomeronasal 1 Receptor 1 (VN1R1) 1 mg/ml IHC Rabbit polyclonal 250
TA200282 Vomeronasal 1 Receptor 1 (VN1R1) 1 mg/ml IHC Rabbit polyclonal 250
TA200285 G Protein-Coupled Receptor GPR44 (CRTH2) 1 mg/ml IHC, ICC Rabbit polyclonal 200
TA200286 G Protein-Coupled Receptor GPR44 (CRTH2) 1 mg/ml IHC, ICC Rabbit polyclonal 300
TA200287 C-C Chemokine Receptor 6 (CCR6) 1 mg/ml IHC Rabbit polyclonal 300
TA200289 Retinoic Acid-Induced 3 (RAI3/RAIG1) 1 mg/ml IHC, ICC Rabbit polyclonal 250
TA200292 G Protein-Coupled Receptor GPR48 1 mg/ml IHC Rabbit polyclonal 250
TA200288 Retinoic Acid-Induced 3 (RAI3/RAIG1) 1 mg/ml IHC, ICC Rabbit polyclonal 250
TA200290 C-C Chemokine Receptor 6 (CCR6) 1 mg/ml IHC Rabbit polyclonal 300
TA200293 Bradykinin B1 Receptor 1 mg/ml IHC Rabbit polyclonal 300
TA200294 C-C Chemokine Receptor 8 (CCR8) 1 mg/ml IHC Rabbit polyclonal 300
TA200295 G Protein-Coupled Receptor D6 1 mg/ml IHC Rabbit polyclonal 300
TA200296 G Protein-Coupled Receptor D6 1 mg/ml IHC Rabbit polyclonal 300
TA200297 G Protein-Coupled Receptor G2A 1 mg/ml IHC Rabbit polyclonal 250
TA200298 G Protein-Coupled Receptor G2A 1 mg/ml IHC Rabbit polyclonal 250
TA200299 G Protein-Coupled Receptor GPR27 (SREB1) 1 mg/ml IHC, ICC Rabbit polyclonal 350
TA200300 Urotensin-II Receptor (GPR14) 1 mg/ml IHC Rabbit polyclonal 300
TA200302 Vasopressin V1B Receptor 1 mg/ml IHC Rabbit polyclonal 300
TA200303 Urotensin-II Receptor (GPR14) 1 mg/ml IHC Rabbit polyclonal 300
TA200304 P2Y12 Platelet ADP Receptor 1 mg/ml IHC, ICC Rabbit polyclonal 350
Product offering is subject to change. Restricted availability to international customers. Refer to website for the most updated information.
Sp. Cross Reactivity Key: H=Human M=Mouse Mi=Mink Mk=Monkey R=Rat C=Chicken Hm=Hamster X=Xenopus Z=Zebra Fish
Mis
c.m
ole
cula
rto
ols
Pre-
valid
ated
antib
odie
s
124 www.origene.com
Cat. # Description Format Application Host Sp Cross Reactivity Type Price*
TA200305 Neurotensin Receptor Type 2 (NTR2) 1 mg/ml IHC Rabbit polyclonal 350
TA200340 Somatostatin Receptor Type 4 1 mg/ml IHC Rabbit polyclonal 300
TA200342 Melanocortin 4 Receptor (MC4R) 1 mg/ml IHC Rabbit polyclonal 200
TA200366 G Protein-Coupled Receptor GPR32 1 mg/ml IHC Rabbit polyclonal 350
TA200367 G Protein-Coupled Receptor GPR86/GPR94/P2Y13 1 mg/ml IHC Rabbit polyclonal 250
TA200370 G Protein-Coupled Receptor GPR72 1 mg/ml IHC Rabbit polyclonal 250
TA200306 Lysophosphatidic Acid Receptor Edg4/LPA2 1 mg/ml IHC, ICC Rabbit polyclonal 200
TA200307 G Protein-Coupled Receptor GPR25 1 mg/ml IHC Rabbit polyclonal 350
TA200308 Purinergic Receptor P2Y1 1 mg/ml IHC, ICC Rabbit polyclonal 200
TA200309 Sphingolipid Receptor Edg8/S1P5 1 mg/ml IHC Rabbit polyclonal 250
TA200314 Purinergic Receptor P2Y1 1 mg/ml IHC Rabbit polyclonal 300
TA200315 Purinergic Receptor P2Y1 1 mg/ml IHC, ICC Rabbit polyclonal 300
TA200318 Olfactory Receptor, Family 2, 1 mg/ml IHC Rabbit polyclonal 350
Subfamily A, Member 4 (OR2A4)
TA200310 Gastric Inhibitory Polypeptide Receptor 1 mg/ml IHC Rabbit polyclonal 250
TA200311 Gastric Inhibitory Polypeptide Receptor 1 mg/ml IHC Rabbit polyclonal 350
TA200312 C-C Chemokine Receptor 8 (CCR8) 1 mg/ml IHC Rabbit polyclonal 300
TA200313 Complement Component 5a Receptor 1 1 mg/ml IHC, ICC Rabbit polyclonal 200
TA200316 Prostaglandin E Receptor EP4 1 mg/ml IHC, ICC Rabbit polyclonal 200
TA200317 Prostaglandin E Receptor EP4 1 mg/ml IHC, ICC Rabbit polyclonal 200
TA200319 Prolactin Releasing Hormone Receptor (GPR10) 1 mg/ml IHC Rabbit polyclonal 350
TA200320 Dopamine Receptor D4 (DRD4) 1 mg/ml IHC Rabbit polyclonal 300
TA200324 Neuromedin U Receptor 1 (GPR66) 1 mg/ml IHC Rabbit polyclonal 300
TA200328 Follicle-Stimulating Hormone Receptor (FSHR) 1 mg/ml IHC Rabbit polyclonal 300
TA200329 Endothelin Type B Receptor-Like 1 mg/ml IHC Rabbit polyclonal 350
Protein 2 (ETBR-LP-2)
TA200330 Endothelin Type B Receptor-Like 1 mg/ml IHC Rabbit polyclonal 350
Protein 2 (ETBR-LP-2)
TA200321 G Protein-Coupled Receptor GPR18 1 mg/ml IHC Rabbit polyclonal 250
TA200322 Adrenomedullin Receptor L1 1 mg/ml IHC Rabbit polyclonal 250
TA200323 Adrenomedullin Receptor L1 1 mg/ml IHC Rabbit polyclonal 250
TA200358 G Protein-Coupled Receptor GPR52 1 mg/ml IHC Rabbit polyclonal 350
TA200363 G Protein-Coupled Receptor GPR25 1 mg/ml IHC Rabbit polyclonal 350
TA200364 G Protein-Coupled Receptor GPR86/GPR94/P2Y13 1 mg/ml IHC Rabbit polyclonal 250
TA200325 G Protein-Coupled Receptor GPR35 1 mg/ml IHC, ICC Rabbit polyclonal 250
TA200326 Alpha 2c-Adrenoceptor 1 mg/ml IHC Rabbit polyclonal 200
TA200327 Melanocortin 2 Receptor (ACTH Receptor) 1 mg/ml IHC Rabbit polyclonal 300
TA200331 Endothelin A Receptor 1 mg/ml IHC Rabbit polyclonal 200
TA200332 Endothelin A Receptor 1 mg/ml IHC Rabbit polyclonal 200
TA200334 Endothelin A Receptor 1 mg/ml IHC Rabbit polyclonal 200
TA200335 G Protein-Coupled Receptor GPRC5B 1 mg/ml IHC Rabbit polyclonal 250
TA200338 G Protein-Coupled Receptor GPRC5B 1 mg/ml IHC Rabbit polyclonal 350
TA200339 G Protein-Coupled Receptor GPRC5B 1 mg/ml IHC Rabbit polyclonal 350
TA200341 Frizzled-1 (FZD1) 1 mg/ml IHC Rabbit polyclonal 300
TA200343 Beta-3 Adrenoceptor 1 mg/ml IHC Rabbit polyclonal 300
TA200344 Beta-3 Adrenoceptor 1 mg/ml IHC Rabbit polyclonal 200
TA200345 Beta-3 Adrenoceptor 1 mg/ml IHC Rabbit polyclonal 300
Product offering is subject to change. Restricted availability to international customers. Refer to website for the most updated information.
Sp. Cross Reactivity Key: H=Human M=Mouse Mi=Mink Mk=Monkey R=Rat C=Chicken Hm=Hamster X=Xenopus Z=Zebra Fish
Mis
c.m
ole
cula
rto
ols
Pre-
valid
ated
antib
odie
s
125ph: 1.888.267.4436 fax: 1.301.340.9254
Cat. # Description Format Application Host Sp Cross Reactivity Type Price*
TA200346 Neuromedin U Receptor 2 1 mg/ml IHC Rabbit polyclonal 350
TA200420 Apelin Receptor (APJ/AGTRL1) 1 mg/ml IHC, ICC Rabbit polyclonal 250
TA200422 G Protein-Coupled Receptor GPR105 1 mg/ml IHC Rabbit polyclonal 350
TA200349 Glucagon Receptor 1 mg/ml IHC Rabbit polyclonal 250
TA200350 Glucagon Receptor 1 mg/ml IHC Rabbit polyclonal 250
TA200347 G Protein-Coupled Receptor GPR17 1 mg/ml IHC Rabbit polyclonal 350
TA200348 G Protein-Coupled Receptor GPR17 1 mg/ml IHC, ICC Rabbit polyclonal 250
TA200352 Frizzled-5 (FZD5) 1 mg/ml IHC Rabbit polyclonal 350
TA200353 G Protein-Coupled Receptor GPR92/GPR93 1 mg/ml IHC Rabbit polyclonal 300
TA200354 Frizzled-5 (FZD5) 1 mg/ml IHC Rabbit polyclonal 350
TA200357 Prostacyclin Receptor 1 mg/ml IHC Rabbit polyclonal 350
TA200359 Frizzled-6 (FZD6) 1 mg/ml IHC, ICC Rabbit polyclonal 250
TA200360 Frizzled-6 (FZD6) 1 mg/ml IHC, ICC Rabbit polyclonal 350
TA200361 EBV-Induced Gene 2 1 mg/ml IHC Rabbit polyclonal 350
TA200362 EBV-Induced Gene 2 1 mg/ml IHC Rabbit polyclonal 350
TA200369 Bombesin Receptor Subtype 3 (BRS3) 1 mg/ml IHC Rabbit polyclonal 300
TA200365 Alpha 2c-Adrenoceptor 1 mg/ml IHC Rabbit polyclonal 200
TA200368 Frizzled-4 (FZD4) 1 mg/ml IHC Rabbit polyclonal 350
TA200372 G Protein-Coupled Receptor GPR27 (SREB1) 1 mg/ml IHC, ICC Rabbit polyclonal 350
TA200377 G Protein-Coupled Receptor MRGX3 1 mg/ml IHC Rabbit polyclonal 250
(SNSR1/SNSR2)
TA200378 G Protein-Coupled Receptor MRGX3 1 mg/ml IHC Rabbit polyclonal 350
(SNSR1/SNSR2)
TA200379 SREB3 1 mg/ml IHC Rabbit polyclonal 350
TA200380 Hepatocyte Nuclear Factor 4 Gamma 1 mg/ml IHC Rabbit polyclonal 300
(HNF4 Gamma/NR2A2)
TA200381 Hepatocyte Nuclear Factor 4 Gamma 1 mg/ml IHC Rabbit polyclonal 300
(HNF4 Gamma/NR2A2)
TA200382 Retinoid-Related Orphan Receptor Gamma 1 mg/ml IHC Rabbit polyclonal 250
(ROR Gamma/NR1F3)
TA200383 Retinoid-Related Orphan Receptor Gamma 1 mg/ml IHC Rabbit polyclonal 250
(ROR Gamma/NR1F3)
TA200384 Retinoid-Related Orphan Receptor Gamma 1 mg/ml IHC Rabbit polyclonal 250
(ROR Gamma/NR1F3)
TA200412 Apelin Receptor (APJ/AGTRL1) 1 mg/ml IHC Rabbit polyclonal 350
TA200416 5-HT1E Receptor 1 mg/ml IHC Rabbit polyclonal 250
TA200419 Apelin Receptor (APJ/AGTRL1) 1 mg/ml IHC, ICC Rabbit polyclonal 350
TA200385 Retinoid-Related Orphan Receptor Gamma 1 mg/ml IHC Rabbit polyclonal 350
(ROR Gamma/NR1F3)
TA200386 SREB3 1 mg/ml IHC Rabbit polyclonal 350
TA200387 Nuclear Receptor SHP (NR0B2) 1 mg/ml IHC Rabbit polyclonal 250
TA200397 G Protein-Coupled Receptor GPR26 1 mg/ml IHC Rabbit polyclonal 350
TA200410 Apelin Receptor (APJ/AGTRL1) 1 mg/ml IHC, ICC Rabbit polyclonal 350
TA200423 G Protein-Coupled Receptor GPR43 1 mg/ml IHC Rabbit polyclonal 350
TA200425 G Protein-Coupled Receptor GPR8 1 mg/ml IHC Rabbit polyclonal 350
TA200388 Nuclear Receptor SHP (NR0B2) 1 mg/ml IHC Rabbit polyclonal 350
TA200389 Estrogen-Related Receptor Alpha 1 mg/ml IHC Rabbit polyclonal 350
(ERR Alpha/NR3B1)
Product offering is subject to change. Restricted availability to international customers. Refer to website for the most updated information.
Sp. Cross Reactivity Key: H=Human M=Mouse Mi=Mink Mk=Monkey R=Rat C=Chicken Hm=Hamster X=Xenopus Z=Zebra Fish
Mis
c.m
ole
cula
rto
ols
Pre-
valid
ated
antib
odie
s
126 www.origene.com
Cat. # Description Format Application Host Sp Cross Reactivity Type Price*
TA200390 Nuclear Receptor SHP (NR0B2) 1 mg/ml IHC Rabbit polyclonal 350
TA200391 Estrogen-Related Receptor Alpha 1 mg/ml IHC Rabbit polyclonal 200
(ERR Alpha/NR3B1)
TA200392 G Protein-Coupled Receptor GPCR150 1 mg/ml IHC Rabbit polyclonal 350
TA200393 Estrogen-Related Receptor Alpha 1 mg/ml IHC Rabbit polyclonal 350
(ERR Alpha/NR3B1)
TA200394 Retinoid-Related Orphan Receptor Beta 1 mg/ml IHC Rabbit polyclonal 250
(ROR Beta/NR1F2)
TA200395 Retinoid-Related Orphan Receptor Alpha 1 mg/ml IHC Rabbit polyclonal 350
(ROR Alpha/NR1F1)
TA200396 ErbA-Related Protein EAR2 (NR2F6) 1 mg/ml IHC Rabbit polyclonal 250
TA200398 G Protein-Coupled Receptor LGR7 1 mg/ml IHC Rabbit polyclonal 300
TA200399 G Protein-Coupled Receptor LGR7 1 mg/ml IHC Rabbit polyclonal 300
TA200405 Photoreceptor-Specific Nuclear Receptor 1 mg/ml IHC Rabbit polyclonal 350
(PNR/NR2E3)
TA200406 Photoreceptor-Specific Nuclear Receptor 1 mg/ml IHC Rabbit polyclonal 350
(PNR/NR2E3)
TA200400 G Protein-Coupled Receptor GPRC5D 1 mg/ml IHC Rabbit polyclonal 350
TA200401 ErbA-Related Protein EAR2 (NR2F6) 1 mg/ml IHC Rabbit polyclonal 350
TA200402 G Protein-Coupled Receptor GPRC5D 1 mg/ml IHC Rabbit polyclonal 350
TA200403 ErbA-Related Protein EAR2 (NR2F6) 1 mg/ml IHC Rabbit polyclonal 250
TA200404 ErbA-Related Protein EAR2 (NR2F6) 1 mg/ml IHC Rabbit polyclonal 350
TA200407 5-HT1B Receptor 1 mg/ml IHC Rabbit polyclonal 200
TA200408 Nuclear Receptor Rev-ErbA Alpha (NR1D1) 1 mg/ml IHC Rabbit polyclonal 250
TA200427 Protein Phosphatase MGC1136 1 mg/ml IHC Rabbit polyclonal 350
TA200428 Protein Phosphatase MGC1136 1 mg/ml IHC Rabbit polyclonal 350
TA200463 G Protein-Coupled Receptor GPR128 1 mg/ml IHC Rabbit polyclonal 350
TA200464 Interleukin 8 Receptor B (CXCR2) 1 mg/ml IHC Rabbit polyclonal 200
TA200409 G Protein-Coupled Receptor GPCR150 1 mg/ml IHC Rabbit polyclonal 350
TA200411 G Protein-Coupled Receptor GPR15 1 mg/ml IHC Rabbit polyclonal 300
TA200413 EGF-Like Module-Containing Mucin-Like 1 mg/ml IHC, ICC Rabbit polyclonal 250
Receptor 2 (EMR2)
TA200414 EGF-Like Module-Containing Mucin-Like (EMR2) 1 mg/ml IHC, ICC Rabbit polyclonal 350
Receptor 2
TA200415 G Protein-Coupled Receptor GPR63 (PSP24 Beta) 1 mg/ml IHC Rabbit polyclonal 250
TA200434 FLJ20442 1 mg/ml IHC Rabbit polyclonal 300
TA200417 G Protein-Coupled Receptor GPR63 (PSP24 Beta) 1 mg/ml IHC Rabbit polyclonal 350
TA200418 EGF-Like Module-Containing Mucin-Like (EMR2) 1 mg/ml IHC, ICC Rabbit polyclonal 350
Receptor 2
TA200421 5-HT6 Receptor 1 mg/ml IHC Rabbit polyclonal 300
TA200424 Melatonin-Related Receptor 1 mg/ml IHC Rabbit polyclonal 250
TA200426 Metabotropic Glutamate Receptor 2 1 mg/ml IHC, ICC Rabbit polyclonal 200
TA200429 Protein Tyrosine Phosphatase Alpha (PTPRA) 1 mg/ml IHC Rabbit polyclonal 250
TA200430 Protein Tyrosine Phosphatase Alpha (PTPRA) 1 mg/ml IHC Rabbit polyclonal 250
TA200431 Protein Tyrosine Phosphatase Epsilon (PTPRE) 1 mg/ml IHC Rabbit polyclonal 250
TA200432 Protein Tyrosine Phosphatase Epsilon (PTPRE) 1 mg/ml IHC Rabbit polyclonal 250
TA200433 Protein Tyrosine Phosphatase Epsilon (PTPRE) 1 mg/ml IHC Rabbit polyclonal 250
TA200435 MAP Kinase Phosphatase X (MKPX/JSP-1) 1 mg/ml IHC Rabbit polyclonal 250
Product offering is subject to change. Restricted availability to international customers. Refer to website for the most updated information.
Sp. Cross Reactivity Key: H=Human M=Mouse Mi=Mink Mk=Monkey R=Rat C=Chicken Hm=Hamster X=Xenopus Z=Zebra Fish
Mis
c.m
ole
cula
rto
ols
Pre-
valid
ated
antib
odie
s
127ph: 1.888.267.4436 fax: 1.301.340.9254
Cat. # Description Format Application Host Sp Cross Reactivity Type Price*
TA200436 MAP Kinase Phosphatase X (MKPX/JSP-1) 1 mg/ml IHC Rabbit polyclonal 250
TA200437 G Protein-Coupled Receptor GPR55 1 mg/ml IHC Rabbit polyclonal 350
TA200438 Adenosine A3 Receptor 1 mg/ml IHC Rabbit polyclonal 300
TA200439 Adenosine A3 Receptor 1 mg/ml IHC Rabbit polyclonal 250
TA200440 Alpha 1b-Adrenoceptor 1 mg/ml IHC Rabbit polyclonal 250
TA200443 Estrogen-Related Receptor Beta 1 mg/ml IHC Rabbit polyclonal 350
(ERR Beta/NR3B2)
TA200444 Estrogen-Related Receptor Beta 1 mg/ml IHC Rabbit polyclonal 350
(ERR Beta/NR3B2)
TA200445 Estrogen-Related Receptor Beta 1 mg/ml IHC Rabbit polyclonal 250
(ERR Beta/NR3B2)
TA200441 Alpha 1b-Adrenoceptor 1 mg/ml IHC Rabbit polyclonal 300
TA200442 Sphingolipid Receptor Edg6/S1P4 1 mg/ml IHC Rabbit polyclonal 300
TA200446 Macrophage-Stimulating 1 Receptor (MST1R/Ron) 1 mg/ml IHC Rabbit polyclonal 350
TA200447 Sphingolipid Receptor Edg6/S1P4 1 mg/ml IHC Rabbit polyclonal 300
TA200450 CDC2-Related Protein Kinase 7 (CRK7) 1 mg/ml IHC Rabbit polyclonal 350
TA200480 Lysophosphatidic Acid Receptor 1 mg/ml IHC Rabbit polyclonal 350
P2Y9/LPA4/GPR23
TA200490 Neurotensin Receptor Type 1 1 mg/ml IHC Rabbit polyclonal 300
TA200448 Serum/Glucocorticoid Regulated Kinase-Like 1 mg/ml IHC Rabbit polyclonal 250
(SGKL/SGK3)
TA200449 NIMA (Never In Mitosis Gene A)-Related 1 mg/ml IHC Rabbit polyclonal 250
Kinase 6 (NEK6)
TA200451 G Protein-Coupled Receptor GPR1 1 mg/ml IHC, ICC Rabbit polyclonal 250
TA200452 Misshapen/NIK-Related Kinase (MINK/MAP4K6) 1 mg/ml IHC Rabbit polyclonal 250
TA200453 G Protein-Coupled Receptor GPR1 1 mg/ml IHC, ICC Rabbit polyclonal 350
TA200454 Parathyroid Hormone Receptor 1 (PTHR1) 1 mg/ml IHC Rabbit polyclonal 300
TA200455 G Protein-Coupled Receptor GPR1 1 mg/ml IHC, ICC Rabbit polyclonal 350
TA200456 A Disintegrin-like and Metalloprotease with 1 mg/ml IHC Rabbit polyclonal 350
Thrombospondin Type 1 Motif, 5 (ADAMTS5)
TA200457 G Protein-Coupled Receptor GPR12 1 mg/ml IHC Rabbit polyclonal 350
TA200458 G Protein-Coupled Receptor TM7SF1 1 mg/ml IHC, ICC Rabbit polyclonal 350
TA200459 G Protein-Coupled Receptor TM7SF1 1 mg/ml IHC, ICC Rabbit polyclonal 250
TA200460 G Protein-Coupled Receptor TM7SF1 1 mg/ml IHC Rabbit polyclonal 350
TA200461 MAP Kinase Phosphatase X (MKPX/JSP-1) 1 mg/ml IHC Rabbit polyclonal 250
TA200462 Bradykinin B1 Receptor 1 mg/ml IHC Rabbit polyclonal 300
TA200465 Nuclear Receptor Rev-ErbA Alpha (NR1D1) 1 mg/ml IHC Rabbit polyclonal 250
TA200466 G Protein-Coupled Receptor GPR101 1 mg/ml IHC Rabbit polyclonal 350
TA200467 Neuromedin B Receptor 1 mg/ml IHC Rabbit polyclonal 300
TA200468 Gastrin-Releasing Peptide Receptor 1 mg/ml IHC Rabbit polyclonal 350
TA200469 Growth Hormone Releasing Hormone Receptor 1 mg/ml IHC Rabbit polyclonal 250
TA200470 Growth Hormone Releasing Hormone Receptor 1 mg/ml IHC Rabbit polyclonal 250
TA200471 Growth Hormone Releasing Hormone Receptor 1 mg/ml IHC Rabbit polyclonal 250
TA200472 Glucagon Receptor 1 mg/ml IHC Rabbit polyclonal 350
TA200473 Glucagon Receptor 1 mg/ml IHC Rabbit polyclonal 250
TA200474 Glucagon Receptor 1 mg/ml IHC Rabbit polyclonal 250
TA200475 C-C Chemokine Receptor 7 (CCR7) 1 mg/ml IHC Rabbit polyclonal 300
TA200477 Purinergic Receptor P2Y10 1 mg/ml IHC Rabbit polyclonal 250
Product offering is subject to change. Restricted availability to international customers. Refer to website for the most updated information.
Sp. Cross Reactivity Key: H=Human M=Mouse Mi=Mink Mk=Monkey R=Rat C=Chicken Hm=Hamster X=Xenopus Z=Zebra Fish
Mis
c.m
ole
cula
rto
ols
Pre-
valid
ated
antib
odie
s
128 www.origene.com
Cat. # Description Format Application Host Sp Cross Reactivity Type Price*
TA200478 Purinergic Receptor P2Y10 1 mg/ml IHC Rabbit polyclonal 350
TA200479 Purinergic Receptor P2Y10 1 mg/ml IHC Rabbit polyclonal 350
TA200476 C-C Chemokine Receptor 7 (CCR7) 1 mg/ml IHC, ICC Rabbit polyclonal 200
TA200481 Metabotropic Glutamate Receptor 1 1 mg/ml IHC Rabbit polyclonal 200
TA200482 G Protein-Coupled Receptor GPR17 1 mg/ml IHC, ICC Rabbit polyclonal 350
TA200483 Metabotropic Glutamate Receptor 1 1 mg/ml IHC Rabbit polyclonal 200
TA200484 Metabotropic Glutamate Receptor 2 1 mg/ml IHC, W Rabbit polyclonal 200
TA200485 Metabotropic Glutamate Receptor 2 1 mg/ml IHC, ICC, W Rabbit polyclonal 200
TA200486 Metabotropic Glutamate Receptor 4 1 mg/ml IHC Rabbit polyclonal 300
TA200487 Metabotropic Glutamate Receptor 7 1 mg/ml IHC Rabbit polyclonal 250
TA200488 Metabotropic Glutamate Receptor 7 1 mg/ml IHC Rabbit polyclonal 250
TA200489 Metabotropic Glutamate Receptor 7 1 mg/ml IHC Rabbit polyclonal 350
TA200492 G Protein-Coupled Receptor GPR18 1 mg/ml IHC Rabbit polyclonal 350
TA200493 PACAP Receptor Type 1 1 mg/ml IHC Rabbit polyclonal 350
TA200494 C-C Chemokine Receptor 9 (CCR9) 1 mg/ml IHC Rabbit polyclonal 300
TA200495 C-C Chemokine Receptor 9 (CCR9) 1 mg/ml IHC Rabbit polyclonal 300
TA200496 G Protein-Coupled Receptor GPR19 1 mg/ml IHC Rabbit polyclonal 350
TA200497 G Protein-Coupled Receptor GPR19 1 mg/ml IHC Rabbit polyclonal 350
TA200498 Somatostatin Receptor Type 1 1 mg/ml IHC Rabbit polyclonal 300
TA200499 Somatostatin Receptor Type 1 1 mg/ml IHC Rabbit polyclonal 300
TA200500 Somatostatin Receptor Type 2 1 mg/ml IHC Rabbit polyclonal 300
TA201012 Histone Deacetylase 5 (HDAC5) Antibody 100 µL (10 WB) W IP IHC IC Rabbit H Mk M R Polyclonal 150
TA201013 Alpha-Fodrin Antibody 100 µL (10 WB) W IC Rabbit H Polyclonal 150
TA201092 p21 Waf1/Cip1 (DCS60) Mouse mAb 100 µL (10 WB) W IP IHC Mouse H Mk Monoclonal 150
TA201093 Smac/Diablo Mouse mAb 100 µL (10 WB) W IP IC Mouse H Monoclonal 150
TA201094 Akt2 Antibody 100 µL (10 WB) W IP Rabbit H M R Polyclonal 150
TA201095 Akt2 (5B5) Rabbit mAb 100 µL (10 WB) W IP Rabbit H M R Mk Monoclonal 175
TA201096 Akt (5G3) Mouse mAb 100 µL (10 WB) IP IC Mouse H M R Hm Monoclonal 150
TA201097 Akt1 (2H10) Mouse mAb 100 µL (10 WB) W IP IHC F Mouse H M R Monoclonal 150
TA201098 SUV39H1 Histone Methyltransferase Antibody 100 µL (10 WB) W Rabbit H Polyclonal 150
TA201099 p95/NBS1 Antibody 100 µL (10 WB) W IC Rabbit H Mi Mk M R Polyclonal 150
TA201100 NAK Antibody 100 µL (10 WB) W IC Rabbit H M R Polyclonal 150
TA201101 IGF-I Receptor alpha Antibody 100 µL (10 WB) W IP IHC IC Rabbit H M R Polyclonal 150
TA201102 NF-kappaB p65 Antibody 100 µL (10 WB) W Rabbit H M R Polyclonal 150
TA201103 NF-kappaB p105/p50 Antibody 100 µL (10 WB) W IHC Rabbit H Mk Polyclonal 150
TA201104 MAPKAPK-2 Antibody 100 µL (10 WB) W Rabbit H M R (Hm) Polyclonal 150
TA201105 LKB1 Antibody 100 µL (10 WB) W IC Rabbit H R Polyclonal 150
TA201106 PDK1 Antibody 100 µL (10 WB) W IP IHC Rabbit H M R Polyclonal 150
TA201107 PKR Antibody 100 µL (10 WB) W IP IC Rabbit H M Polyclonal 150
TA201108 C/EBPbeta Antibody 100 µL (10 WB) W Rabbit R Polyclonal 150
TA201109 C/EBPbeta (LAP) Antibody 100 µL (10 WB) W Rabbit H M (R) Polyclonal 150
TA201110 Aurora A/AIK Antibody 100 µL (10 WB) W IC Rabbit H M Mk Polyclonal 150
TA201111 Aurora B/AIM1 Antibody 100 µL (10 WB) W Rabbit H M R Mk Polyclonal 150
TA201112 Smad2 Antibody 100 µL (10 WB) W IP IC Rabbit H M R Polyclonal 150
TA201113 VASP Antibody 100 µL (10 WB) W IHC Rabbit H Mk Polyclonal 150
TA201114 Met (25H2) Mouse mAb 100 µL (10 WB) W IP Mouse H M R Mk Monoclonal 150
Product offering is subject to change. Restricted availability to international customers. Refer to website for the most updated information.
Sp. Cross Reactivity Key: H=Human M=Mouse Mi=Mink Mk=Monkey R=Rat C=Chicken Hm=Hamster X=Xenopus Z=Zebra Fish
Mis
c.m
ole
cula
rto
ols
Pre-
valid
ated
antib
odie
s
129ph: 1.888.267.4436 fax: 1.301.340.9254
Cat. # Description Format Application Host Sp Cross Reactivity Type Price*
TA201115 cdk6 (DCS83) Mouse mAb 100 µL (10 WB) W IHC Mouse H M R Monoclonal 150
TA201116 Ezrin/Radixin/Moesin Antibody 100 µL (10 WB) W IHC IC Rabbit W IHC IC Polyclonal 150
TA201117 PDGF Receptor beta Antibody 100 µL (10 WB) W Rabbit H M R Polyclonal 150
TA201118 Progesterone Receptor (6A1) 100 µL (10 WB) W IP IC Mouse H Monoclonal 150
Monoclonal Antibody
TA201119 CrkL (32H4) Mouse mAb 100 µL (10 WB) W F Mouse H R Hm Monoclonal 150
TA201120 Androgen Receptor Antibody 100 µL (10 WB) W IHC IC Rabbit H Polyclonal 150
TA201121 Etk Antibody 100 µL (10 WB) W Rabbit H M R Polyclonal 150
TA201122 Gab1 Antibody 100 µL (10 WB) W IHC IP Rabbit H M R Mk Polyclonal 150
TA201123 PAK4 Antibody 100 µL (10 WB) W IP IHC Rabbit H M R Mk Polyclonal 150
TA201124 Blk Antibody 100 µL (10 WB) W Rabbit H Polyclonal 150
TA201125 SGK Antibody 100 µL (10 WB) W IP IC Rabbit H M R Polyclonal 150
TA201126 Pyk2 Antibody 100 µL (10 WB) W IP Rabbit H M Polyclonal 150
TA201127 Cofilin Antibody 100 µL (10 WB) W IP Rabbit H M R Hm Mk Polyclonal 150
TA201128 Ras-GRF1 Antibody 100 µL (10 WB) W IHC Rabbit M Polyclonal 150
TA201129 Jak1 Antibody 100 µL (10 WB) W IP Rabbit H M Polyclonal 150
TA201130 ALK Antibody 100 µL (10 WB) W IP Rabbit H Polyclonal 150
TA201131 Stathmin Antibody 100 µL (10 WB) W IHC Rabbit H Mk M R Polyclonal 150
TA201132 CaMKII Antibody 100 µL (10 WB) W Rabbit H M R Mk Polyclonal 150
TA201133 Erk5 Antibody 100 µL (10 WB) W IP Rabbit H M R Mk Polyclonal 150
TA201134 c-Kit Antibody 100 µL (10 WB) W IP Rabbit H M Polyclonal 150
TA201135 Rad17 Antibody 100 µL (10 WB) W IHC IC Rabbit H Mi Mk M R Polyclonal 150
TA201136 Cytokine Receptor Common beta-Chain Antibody 100 µL (10 WB) W Rabbit H M R Mi Mk Polyclonal 150
TA201137 Phospho-FLT3 (Tyr589/591) (30D4) Rabbit mAb 100 µL (10 WB) W Rabbit M (H) Monoclonal 275
TA201138 FGF Receptor 1 Antibody 100 µL (10 WB) W IP Rabbit H (M) (R) Polyclonal 150
TA201139 CrkII Antibody 100 µL (10 WB) W IC Rabbit H M Polyclonal 150
TA201140 Cortactin Antibody 100 µL (10 WB) W IHC Rabbit H Mk M R Polyclonal 150
TA201141 Connexin 43 Antibody 100 µL (10 WB) W IHC Rabbit H M R Mk Polyclonal 150
TA201142 Btk Antibody 100 µL (10 WB) W Rabbit H M Polyclonal 150
TA201143 NPM Antibody 100 µL (10 WB) W IHC Rabbit H M R Polyclonal 150
TA201144 CD19 Antibody 100 µL (10 WB) W IP IC Rabbit H M Polyclonal 150
TA201145 eIF4B Antibody 100 µL (10 WB) W IHC IC Rabbit H M R Mk Polyclonal 150
TA201146 BLNK Antibody 100 µL (10 WB) W Rabbit H M Polyclonal 150
TA201147 Tuberin Antibody 100 µL (10 WB) W Rabbit H M R Mk Polyclonal 150
TA201148 Presenilin 1 Antibody 200 µL (10 WB) W IP Rabbit H R Mk (M) Polyclonal 150
TA201149 Nicastrin Antibody 100 µL (10 WB) W Rabbit H M R Mk Polyclonal 150
TA201150 Myosin Light Chain 2 Antibody 100 µL (10 WB) W Rabbit H M R (C) Polyclonal 150
TA201151 Mst1 Antibody 100 µL (10 WB) W IP IHC Rabbit H Mk M R Polyclonal 150
TA201152 eEF2k Antibody 100 µL (10 WB) W IP IC Rabbit H R Mk Polyclonal 150
TA201153 GRB10 Antibody 100 µL (10 WB) W IP E IHC Rabbit H Hm Polyclonal 150
TA201154 TGF-beta Receptor I Antibody 100 µL (10 WB) W IHC Rabbit H Mi R Polyclonal 150
TA201155 Pin1 Antibody 100 µL (10 WB) W Rabbit H Mk M R (X) Polyclonal 150
TA201156 GP130 Antibody 100 µL (10 WB) W Rabbit H M R Mk Polyclonal 150
TA201157 SHP-2 Antibody 100 µL (10 WB) W IP IHC Rabbit H M R Polyclonal 150
TA201158 GCK Antibody 100 µL (10 WB) W IP IHC IC Rabbit H M R Mk Polyclonal 150
TA201159 Bak Antibody 100 µL (10 WB) W IP IC Rabbit H Mk Polyclonal 150
Product offering is subject to change. Restricted availability to international customers. Refer to website for the most updated information.
Sp. Cross Reactivity Key: H=Human M=Mouse Mi=Mink Mk=Monkey R=Rat C=Chicken Hm=Hamster X=Xenopus Z=Zebra Fish
Mis
c.m
ole
cula
rto
ols
Pre-
valid
ated
antib
odie
s
130 www.origene.com
Cat. # Description Format Application Host Sp Cross Reactivity Type Price*
TA201160 LSP1 Antibody 100 µL (10 WB) W Rabbit H M R Polyclonal 150
TA201161 Mst4 Antibody 100 µL (10 WB) W Rabbit H Mk M R Polyclonal 150
TA201162 PLD1 Antibody 100 µL (10 WB) W IP Rabbit H M R Polyclonal 150
TA201163 LIMK1 Antibody 100 µL (10 WB) W IP Rabbit H Mk M R Polyclonal 150
TA201164 LIMK2 Antibody 100 µL (10 WB) W Rabbit H Mk R (M) Polyclonal 150
TA201165 MMP-9 Antibody 100 µL (10 WB) W IHC Rabbit H Polyclonal 150
TA201166 ILK1 Antibody 100 µL (10 WB) W IHC Rabbit H M R Mk Polyclonal 150
TA201167 PLCgamma2 Antibody 100 µL (10 WB) W IP Rabbit H M (R) Polyclonal 150
TA201168 Bcr Antibody 100 µL (10 WB) W F IC Rabbit H M R Polyclonal 150
TA201169 p62Dok Antibody 100 µL (10 WB) W Rabbit H Polyclonal 150
TA201170 p56Dok-2 Antibody 100 µL (10 WB) W Rabbit H M Polyclonal 150
TA201171 IGFBP-2 Antibody 100 µL (10 WB) W IP IHC Rabbit H Polyclonal 150
TA201172 Mst2 Antibody 100 µL (10 WB) W IP Rabbit H Mk M R Polyclonal 150
TA201173 R-Ras Antibody 100 µL (10 WB) W IP Rabbit H M R Mk Polyclonal 150
TA201174 Ras Antibody 100 µL (10 WB) W Rabbit H M R Mk (C) (Hm) (X) (Z) Polyclonal 150
TA201175 GRB2 Antibody 100 µL (10 WB) W IHC Rabbit H M R Hm Mk Polyclonal 150
TA201176 GRK2 Antibody 100 µL (10 WB) W IP Rabbit H M R Hm Mk Polyclonal 150
TA201177 G Protein alpha Subunit Antibody 100 µL (10 WB) W IP IHC Rabbit H Mk M R Polyclonal 150
TA201178 Troponin I Antibody 100 µL (10 WB) W Rabbit M (H) (R) Polyclonal 150
TA201179 MMP-2 Antibody 100 µL (10 WB) W Rabbit H Polyclonal 150
TA201180 CaMKIV Antibody 100 µL (10 WB) W IP Rabbit H M R Polyclonal 150
TA201181 TCL1 Antibody 100 µL (10 WB) W IHC F Rabbit H (M) Polyclonal 150
TA201182 Akt3 Antibody 100 µL (10 WB) W IP Rabbit H M R Polyclonal 150
TA201183 Mst3b Antibody 100 µL (10 WB) W Rabbit H Mk M R Polyclonal 150
TA201184 PDE5 Antibody 100 µL (10 WB) W Rabbit H M R Mk Polyclonal 150
TA201185 ROR1 Antibody 100 µL (10 WB) W Rabbit M (H) Polyclonal 150
TA201186 ROR2 Antibody 100 µL (10 WB) W IP IC Rabbit M (H) Polyclonal 150
TA201187 Cyclin B1 (V152) Mouse mAb 100 µL (10 WB) W IC F Mouse H M (Hm) Monoclonal 150
TA201188 EGR1 Antibody 100 µL (10 WB) W IP Rabbit R (H) (M) Polyclonal 150
TA201189 MKK7 Antibody 100 µL (10 WB) W IP Rabbit H M R Mk Polyclonal 150
TA201190 AMPKbeta1 Antibody 100 µL (10 WB) W IP Rabbit H M R Mk Polyclonal 150
TA201191 CDCP1 Antibody 100 µL (10 WB) W Rabbit H Mk Polyclonal 150
TA201192 Tie2 (AB33) Mouse mAb 100 µL (10 WB) W IP Mouse H Monoclonal 150
TA201193 BCL6 Antibody 100 µL (10 WB) W Rabbit H M (R) Polyclonal 150
TA201194 PI3 Kinase p110 gamma Antibody 100 µL (10 WB) W IP Rabbit H M Polyclonal 150
TA201195 PI3 Kinase p110 alpha Antibody 100 µL (10 WB) W IHC Rabbit H M R Polyclonal 150
TA201196 Cytochrome c Antibody 100 µL (10 WB) W IHC Rabbit H M R Mk Polyclonal 150
TA201197 Myt1 Antibody 100 µL (10 WB) W IHC Rabbit H M R (X) Polyclonal 150
TA201198 PI3 Kinase p85 Antibody 100 µL (10 WB) W Rabbit H M R Polyclonal 150
TA201199 ATP-Citrate Lyase Antibody 100 µL (10 WB) W IP Rabbit H M R Mk Polyclonal 150
TA201200 IRAK Antibody 100 µL (10 WB) W Rabbit H Polyclonal 150
TA201201 p44 MAP Kinase (Erk1) Antibody 100 µL (10 WB) W Rabbit M R Polyclonal 150
TA201202 A-Raf Antibody 100 µL (10 WB) W IP Rabbit H M R Polyclonal 150
TA201203 Apaf-1 Antibody 100 µL (10 WB) W Rabbit H M R Mk Polyclonal 150
TA201204 Ran Antibody 100 µL (10 WB) W Rabbit H M R Hm Mk Polyclonal 150
TA201205 HPK1 Antibody 100 µL (10 WB) W IP Rabbit H M Polyclonal 150
Product offering is subject to change. Restricted availability to international customers. Refer to website for the most updated information.
Sp. Cross Reactivity Key: H=Human M=Mouse Mi=Mink Mk=Monkey R=Rat C=Chicken Hm=Hamster X=Xenopus Z=Zebra Fish
Mis
c.m
ole
cula
rto
ols
Pre-
valid
ated
antib
odie
s
131ph: 1.888.267.4436 fax: 1.301.340.9254
Cat. # Description Format Application Host Sp Cross Reactivity Type Price*
TA201206 PTPmu (BK2) Mouse mAb 100 µL (10 WB) W IP Mouse H R Mi (M) Monoclonal 150
TA201207 Tpl2 Antibody 100 µL (10 WB) W Rabbit R (H) (M) Polyclonal 150
TA201208 IRS-2 Antibody 100 µL (10 WB) W IC Rabbit H Polyclonal 150
TA201209 Bok Antibody 100 µL (10 WB) W IP IHC Rabbit H M R Mk Polyclonal 150
TA201210 Daxx Antibody 100 µL (10 WB) W IP Rabbit H M R Polyclonal 150
TA201211 MAP2 Antibody 100 µL (10 WB) W Rabbit H R (M) Polyclonal 150
TA201212 TFII-I Antibody 100 µL (10 WB) W IP IHC Rabbit H M Mk Polyclonal 150
TA201213 Bim Antibody 100 µL (10 WB) W IHC IC Rabbit H M R Mk Polyclonal 150
TA201214 Bik Antibody 100 µL (10 WB) W IHC Rabbit H M R Polyclonal 150
TA201215 DNA-PK Antibody 100 µL (10 WB) W Rabbit H Polyclonal 150
TA201216 CTMP Antibody 100 µL (10 WB) W IP F Rabbit H M R Polyclonal 150
TA201217 A1/Bfl-1 Antibody 100 µL (10 WB) W Rabbit H M Polyclonal 150
TA201218 AIF Antibody 100 µL (10 WB) W IP IHC IC Rabbit H M R Polyclonal 150
TA201219 Cyclin A (BF683) Mouse mAb 100 µL (10 WB) W F Mouse H Monoclonal 150
TA201220 p73 Antibody 100 µL (10 WB) W IC Rabbit H (M) (Mk) Polyclonal 150
TA201221 JNK2 Antibody 100 µL (10 WB) W Rabbit H M R Mk Polyclonal 150
TA201222 Mad-1 Antibody 100 µL (10 WB) W Rabbit H M R Polyclonal 150
TA201223 Bmf Antibody 100 µL (10 WB) W Rabbit H M R Mk Polyclonal 150
TA201224 Integrin beta3 Antibody 100 µL (10 WB) W IP IHC Rabbit H M (R) Polyclonal 150
TA201225 TRAF2 Antibody 100 µL (10 WB) W Rabbit H M R Polyclonal 150
TA201226 Pim-1 Antibody 100 µL (10 WB) W Rabbit H M R Polyclonal 150
TA201227 Max Antibody 100 µL (10 WB) W IP Rabbit H M R Polyclonal 150
TA201228 RKIP Antibody 100 µL (10 WB) W Rabbit H M R Mk Polyclonal 150
TA201229 VHR Antibody 100 µL (10 WB) W IP Rabbit H M R Mk Polyclonal 150
TA201230 Filamin A Antibody 100 µL (10 WB) W IP Rabbit H M R Mk Polyclonal 150
TA201231 PKA C-alpha Antibody 100 µL (10 WB) W IP F Rabbit H M R Polyclonal 150
TA201232 SMC1 Antibody 100 µL (10 WB) W Rabbit H M (R) (C) (X) Polyclonal 150
TA201233 p15 INK4B Antibody 100 µL (10 WB) W F Rabbit H M R Polyclonal 150
TA201234 Cox2 Antibody 100 µL (10 WB) W IP IHC F Rabbit M (H) (R) Polyclonal 150
TA201235 PCTAIRE 1 Antibody 100 µL (10 WB) W IP F Rabbit M R (H) Polyclonal 150
TA201236 HSP70 Antibody 100 µL (10 WB) W IHC Rabbit H M R Mk Polyclonal 150
TA201237 HSP90 Antibody 100 µL (10 WB) W IHC Rabbit H M R Mk (C) (Z) Polyclonal 150
TA201238 p44/42 MAP Kinase Antibody 200 µL (20 WB) W IHC F Rabbit H M R Hm Z Polyclonal 150
TA201239 p42 MAP Kinase (3A7) Mouse mAb 100 µL (10 WB) W IHC F Mouse H M R Hm Monoclonal 150
TA201240 p42 MAP Kinase (Erk2) Antibody 100 µL (10 WB) W IHC Rabbit H M R Mk Polyclonal 150
TA201241 cdc2 Antibody 100 µL (10 WB) W Rabbit H M R Polyclonal 150
TA201242 cdc2 (POH1) Mouse mAb 100 µL (10 WB) W IP IHC IC Mouse H Mk Monoclonal 150
TA201243 MEK1/2 Antibody 100 µL (10 WB) W F Rabbit H M R Polyclonal 150
TA201244 MEK1 Antibody 100 µL (10 WB) W Rabbit H M R Mk Polyclonal 150
TA201245 MEK2 Antibody 100 µL (10 WB) W IP Rabbit H M R Mk Polyclonal 150
TA201246 Stat3 Antibody 100 µL (10 WB) W IP IHC IC F Rabbit H M R Polyclonal 150
TA201247 Stat3 (124H6) Mouse mAb 100 µL (10 WB) W IP IHC F IC Mouse H M R Mk Monoclonal 150
TA201248 SEK1/MKK4 Antibody 100 µL (10 WB) W IP Rabbit H M R Mk Polyclonal 150
TA201249 c-Jun Antibody 100 µL (10 WB) W Rabbit H M R Polyclonal 150
TA201250 c-Jun (60A8) Rabbit mAb 100 µL (10 WB) W IP IHC Rabbit H M R Mk Monoclonal 175
TA201251 Stat1 Antibody 100 µL (10 WB) W IP F Rabbit H M R Polyclonal 150
Product offering is subject to change. Restricted availability to international customers. Refer to website for the most updated information.
Sp. Cross Reactivity Key: H=Human M=Mouse Mi=Mink Mk=Monkey R=Rat C=Chicken Hm=Hamster X=Xenopus Z=Zebra Fish
Mis
c.m
ole
cula
rto
ols
Pre-
valid
ated
antib
odie
s
132 www.origene.com
Cat. # Description Format Application Host Sp Cross Reactivity Type Price*
TA201252 Stat1 (9H2) Mouse mAb 100 µL (10 WB) W IP IHC IC Mouse H M Monoclonal 150
TA201253 Elk-1 Antibody 100 µL (10 WB) W IHC Rabbit H M R Polyclonal 150
TA201254 CREB Antibody 100 µL (10 WB) W IP IHC IC Rabbit H M R C Polyclonal 150
TA201255 p70 S6 Kinase Antibody 100 µL (10 WB) W IP IC F Rabbit H M R Mk Polyclonal 150
TA201256 p38 MAP Kinase Antibody 100 µL (10 WB) W IHC F Rabbit H M R Polyclonal 150
TA201257 p38 MAP Kinase delta Antibody 100 µL (10 WB) W IP Rabbit H M R Mk Polyclonal 150
TA201258 p38 MAP Kinase (5F11) Mouse mAb 100 µL (10 WB) W IP F Mouse H M R Monoclonal 150
TA201259 p38 MAP Kinase alpha Antibody 100 µL (10 WB) W IP IHC Rabbit H M R Mk Polyclonal 150
TA201260 ATF-2 (20F1) Rabbit mAb 100 µL (10 WB) W IP IHC Rabbit H M R Mk Monoclonal 175
TA201261 MKK3 Antibody 100 µL (10 WB) W Rabbit H M R Polyclonal 150
TA201262 IkappaB-alpha Antibody 100 µL (10 WB) W IP IHC IC Rabbit H M R Polyclonal 150
TA201263 IkappaB-alpha (112B2) Mouse mAb 100 µL (10 WB) W IP IHC Mouse H R Mk (M) Monoclonal 150
TA201264 IkappaB beta Antibody 100 µL (10 WB) W IHC Rabbit H M R Polyclonal 150
TA201265 IkappaB-epsilon Antibody 100 µL (10 WB) W Rabbit H M R Mk Polyclonal 150
TA201266 SAPK/JNK Antibody 100 µL (10 WB) W IC F Rabbit H M R Z Polyclonal 150
TA201267 SAPK/JNK (56G8) Rabbit mAb 100 µL (10 WB) W IP Rabbit H M R Mi Mk Monoclonal 175
TA201268 Akt Antibody 100 µL (10 WB) W IP F Rabbit H M R C Hm Polyclonal 150
TA201269 p53 Antibody 100 µL (10 WB) W IP IC Rabbit H R Mk Polyclonal 150
TA201270 Bad Antibody 100 µL (10 WB) W IP IHC IC Rabbit H M R Mk Polyclonal 150
TA201271 Rb (4H1) Mouse mAb 100 µL (10 WB) W IP IHC IC Mouse H R Mk Monoclonal 150
TA201272 GSK-3beta Antibody 100 µL (10 WB) W IHC IC Rabbit H M R (X) (Z) Polyclonal 150
TA201273 RSK1/RSK2/RSK3 Antibody 100 µL (10 WB) W Rabbit H M R Mk (C) Polyclonal 150
TA201274 Stat5 Antibody 100 µL (10 WB) W IP Rabbit H M Polyclonal 150
TA201275 Stat6 Antibody 100 µL (10 WB) W IP Rabbit H M R Polyclonal 150
TA201276 c-Myc Antibody 100 µL (10 WB) W IP IC Rabbit H M R Polyclonal 150
TA201277 4E-BP1 Antibody 100 µL (10 WB) W IP IHC IC Rabbit H M R Mk Polyclonal 150
TA201278 FKHR Antibody 100 µL (10 WB) W IHC Rabbit H M R Polyclonal 150
TA201279 AFX Antibody 100 µL (10 WB) W Rabbit H M R Polyclonal 150
TA201280 Caspase-7 Antibody 100 µL (10 WB) W F Rabbit H M R Polyclonal 150
TA201281 Caspase-9 Antibody (Human Specific) 100 µL (10 WB) W F Rabbit H Polyclonal 150
TA201282 Caspase-9 Antibody (Mouse Specific) 100 µL (10 WB) W IHC Rabbit M Polyclonal 150
TA201283 Caspase-9 Antibody (Rat Specific) 100 µL (10 WB) W IC Rabbit R Polyclonal 150
TA201284 Smad1 Antibody 100 µL (10 WB) W Rabbit H Mk Polyclonal 150
TA201285 Smad4 Antibody 100 µL (10 WB) W Rabbit H M R Mk Polyclonal 150
TA201286 Smad5 Antibody 100 µL (10 WB) W Rabbit H M R Mk Polyclonal 150
TA201287 cdc25C Antibody 100 µL (10 WB) W IHC Rabbit H Polyclonal 150
TA201288 cdc25B Antibody 100 µL (10 WB) W IHC Rabbit H Mk M R Polyclonal 150
TA201289 PARP Antibody 100 µL (10 WB) W IHC Rabbit H M R Polyclonal 150
TA201290 PTEN Antibody 100 µL (10 WB) W IP Rabbit H M R Hm Mk Polyclonal 150
TA201291 PTEN (26H9) Mouse mAb 100 µL (10 WB) W IP IHC Mouse H M R Hm Mk Monoclonal 150
TA201292 Beta-Catenin Antibody 100 µL (10 WB) W IP IHC Rabbit H M R Mk Polyclonal 150
TA201293 eNOS Antibody 100 µL (10 WB) W Rabbit H (M) (R) Polyclonal 150
TA201294 Caspase-3 Antibody 100 µL (10 WB) W IP IHC IC F Rabbit H M R Polyclonal 150
TA201295 Caspase-3 (8G10) Rabbit Monoclonal Antibody 100 µL (10 WB) W IP IC Rabbit H M R Monoclonal 175
TA201296 Caspase-3 (3G2 ) Mouse mAb 100 µL (10 WB) W Mouse H Monoclonal 150
TA201297 Pro-TGF-alpha Antibody 100 µL (10 WB) W Rabbit H M (R) Polyclonal 150
Product offering is subject to change. Restricted availability to international customers. Refer to website for the most updated information.
Sp. Cross Reactivity Key: H=Human M=Mouse Mi=Mink Mk=Monkey R=Rat C=Chicken Hm=Hamster X=Xenopus Z=Zebra Fish
Mis
c.m
ole
cula
rto
ols
Pre-
valid
ated
antib
odie
s
133ph: 1.888.267.4436 fax: 1.301.340.9254
Cat. # Description Format Application Host Sp Cross Reactivity Type Price*
TA201298 eIF2alpha Antibody 100 µL (10 WB) W Rabbit H M R Polyclonal 150
TA201299 DFF45/DFF35 Antibody 100 µL (10 WB) W IP Rabbit H Polyclonal 150
TA201300 eIF4E Antibody 100 µL (10 WB) W IP Rabbit H M R Z Polyclonal 150
TA201301 Caspase-8 (1C12) Mouse mAb 100 µL (10 WB) W IP F Mouse H Monoclonal 195
TA201302 Caspase-10 Antibody 100 µL (10 WB) W IHC Rabbit H M R Polyclonal 150
TA201303 Caspase-6 Antibody 100 µL (10 WB) W Rabbit H M R Polyclonal 150
TA201304 MEF2C Antibody 100 µL (10 WB) W Rabbit H M R Mk Polyclonal 150
Product offering is subject to change. Restricted availability to international customers. Refer to website for the most updated information.
Sp. Cross Reactivity Key: H=Human M=Mouse Mi=Mink Mk=Monkey R=Rat C=Chicken Hm=Hamster X=Xenopus Z=Zebra Fish
Mis
c.m
ole
cula
rto
ols
Prot
ein
colle
ctio
ns
134 www.origene.com
Protein collections
Introduction
The OriGene Protein Collection contains over 100
purified recombinant proteins for scientist to per-
form protein-based applications. The proteins are
mostly associated with transcription, nuclear re-
ceptors and oncology. Functionally active proteins
can be overexpressed in and purified from several
different systems that have been well characterized.
OriGene's manufacturer primarily utilizes bacterial
and baculoviral systems and has produced over 100
transcription and cancer related proteins.
OriGene, through its partnership with an expert
protein producer, is actively preparing functional
proteins every day for our catalog and to address our
customer's bulk protein needs. Customers are
encouraged to email and request that their protein of
interest be placed on the priority list.
On our website, the proteins provided can be
searched by the following criteria,
• Nucleotide Sequence, Protein Sequence
• Accession or Catalog Number
• Protein Domain
• Keywords
• Gene Family
Product Application
Whereas a human gene transcript supplies the
required information to blueprint the protein
sequence, in order to test cellular function, it is
necessary to produce and test the actual protein.
OriGene has partnered with quality, human protein
providers who derive value from OriGenes large
transcript repository. These high-quality protein
samples are made accessible through this website.
Enzymatic Assays: Protein enzymes can be used to
catalyze reactions of interest in search of substrate
specificity. The researcher can also use such assays
to assess both activation and inhibition of the
enzymes activity. OriGene provides many quality
protein kinases to test their phosphorylation activity.
Protein Binding Studies: Having a properly folded
protein is key to determine its biologically relevant
interactions. This type of study is traditionally
performed through gel-shift assays, ELISA plate
analysis, and various types of affinity chromatography.
Western Blots and SDS-PAGE:The identification of a
proteins rate of migration (Mr) through SDS-PAGE is
best identified through the use of a purified protein.
The proteins available through OriGene are suitable
for this purpose.
Functional Studies: In addition to enzymatic or
binding assays, the researcher can uncover the
cellular function of these proteins through a variety
of specialized assays measuring fluctuations in
second messengers calcium or camp. Transcriptional
regulation can be assessed through off-the-shelf
reporter gene assays.
Quality Control
Functionally active proteins are over expressed in
and purified from several different systems that
have been well characterized. Proteins provided
through OriGene are primarily derived from
bacterial and baculoviral systems to produce
proteins with a focus on transcription and cancer
related proteins. The protein purification strategy
revolves around utilizing protein tags including 6-
histidine, FLAG or myc, to increase purity of the
proteins to near homogeneity upon initial
purification. Initial synthesis involves the creation of
new plasmids containing one or more of the protein
tags that do not interfere with the protein
function(s). This is followed by a second step of
purification (required to further purify recombinant
proteins with a unique protein tag), which will be
decided based on protein structure and property.
These steps involve use of conventional chroma-
tography, fast performance liquid chromatography
Mis
c.m
ole
cula
rto
ols
Prot
ein
colle
ctio
ns
135ph: 1.888.267.4436 fax: 1.301.340.9254
Cat. # Accn Description Unit Source Price*
TP200002 NM_032940 RNA pol II-hRPB5 (RNA polymerase II, 10000 Recombinant human RPB5 is isolated from an E coli 205
p33 subunit) strain that carries the coding sequence of human
RPB5 under the control of a T7 promoter.
TP200003 NM_032940 RNA pol II-hRPB5 (RNA polymerase II, 25000 Recombinant human RPB5 is isolated from an E coli
p33 subunit) strain that carries the coding sequence of human
RPB5 under the control of a T7 promoter. 410
TP200004 X12492 CTF1 CCAAT-Box-Binding Transcription Factor 5000 The wild type human CTF1 protein (499 amino acids) 310
1 (Recombinant Protein from Insect Cells) was expressed in baculovirus system and purified by
using an affinity column and FPLC chromatography.
TP200005 X12492 CTF1 CCAAT-Box-Binding Transcription Factor 12500 The wild type human CTF1 protein (499 amino acids) 620
1 (Recombinant Protein from Insect Cells) was expressed in baculovirus system and purified by
using an affinity column and FPLC chromatography.
TP200006 NM_000551 VHL von Hippel-Lindau tumor suppressor 5000 The wild type human VHL protein (213 amino acids) 268
protein (Recombinant Protein from Insect Cells) was expressed in baculovirus system and purified
using an affinity column and FPLC chromatography
under non-denaturing condition.
TP200007 NM_000551 VHL von Hippel-Lindau tumor suppressor 12500 The wild type human VHL protein (213 amino acids) 534
protein (Recombinant Protein from Insect Cells) was expressed in baculovirus system and purified
using an affinity column and FPLC chromatography
under non-denaturing condition.
TP200008 NM_001514 TFIIB (Transcription Factor IIB) 10000 Isolated from an E coli strain that carries the coding 205
sequence for human TFIIB under the control of T7
promoter (5).
TP200009 NM_001514 TFIIB (Transcription Factor IIB) 25000 Isolated from an E coli strain that carries the coding 410
sequence for human TFIIB under the control of T7
promoter (5).
TP200010 NM_000800 FGF-1 (acidic fibroblast growth factor) 5000 Recombinant FGF1 was expressed in a baculovirus 237
system and purified by an affinity column in
combination with FPLC chromatography.
TP200011 NM_000800 FGF-1 (acidic fibroblast growth factor) 12500 Recombinant FGF1 was expressed in a baculovirus 473
system and purified by an affinity column in
combination with FPLC chromatography.
TP200012 NM_006238 PPAR beta(delta) (Peroxisome proliferator- 10000 Recombinant PPAR is isolated from an E coli strain 204
activated receptor, beta isotype) that carries the coding sequence of the human PPAR
beta under the control of a T7 promoter.
TP200013 NM_006238 PPAR beta(delta) (Peroxisome proliferator- 25000 Recombinant PPAR is isolated from an E coli strain 407
activated receptor, beta isotype) that carries the coding sequence of the human PPAR
beta under the control of a T7 promoter.
TP200014 NM_005225 E2F-1, (RBAP-1) Transcription Factor 5000 The wild type E2F-1 (residue 1-437) was expressed in 310
and Rb-mediator baculovirus system and purified in combination of an
affinity column and FPLC chromatography.
TP200015 NM_005225 E2F-1, (RBAP-1) Transcription Factor 12500 The wild type E2F-1 (residue 1-437) was expressed in 620
and Rb-mediator baculovirus system and purified in combination of an
affinity column and FPLC chromatography.
(FPLC) and / or high-pressure liquid chromatography
(HPLC) based techniques to achieve highly purified
proteins. Quality is monitored by SDS-PAGE.
Proteins are purified greater than 95% homogeneous
and contain no detectable protease, DNase, and
RNase activity.
Product offering is subject to change. See our website for the most updated information.
Product Listing
Mis
c.m
ole
cula
rto
ols
Prot
ein
colle
ctio
ns
136 www.origene.com
Cat. # Accn Description Unit Source Price*
TP200016 NM_002467 c-Myc (proto oncogene) 5000 Recombinant c-myc was expressed in a bacterial 258
system and purified by an affinity column in
combination with FPLC chromatography
TP200017 NM_002467 c-Myc (proto oncogene) 12500 Recombinant c-myc was expressed in a bacterial
system and purified by an affinity column in
combination with FPLC chromatography 515
TP200018 NM_021976 RXR beta (Retinoid X Receptor, beta isoform) 5000 Recombinant RXR beta is expressed in baculovirus 237
system and purified by an affinity column in
combination with FPLC chromatography.
TP200019 NM_021976 RXR beta (Retinoid X Receptor, beta isoform) 12500 Recombinant RXR beta is expressed in baculovirus 473
system and purified by an affinity column in
combination with FPLC chromatography.
TP200020 NM_021144 p52, Transcriptional Coactivator 10000 Recombinant p52 protein (wild type, 333 amino 258
acids) is isolated from an E coli strain that carries
the coding sequence of human p52 under the control
of T7 promoter and purified by the combination of
an affinity column and FPLC chromatography.
TP200021 NM_021144 p52, Transcriptional Coactivator 25000 Recombinant p52 protein (wild type, 333 amino 515
acids) is isolated from an E coli strain that carries
the coding sequence of human p52 under the control
of T7 promoter and purified by the combination
of an affinity column and FPLC chromatography.
TP200022 NM_015859 TFIIA-p55 (Transription Factor IIA, p55 subunit) 10000 p55 subunit of TFIIA is isolated form a strain of 205
E coli that contains the coding sequence of human
p55 of TFIIA under the control of T7 promoter(3).
TP200023 NM_015859 TFIIA-p55 (Transription Factor IIA, p55 subunit) 25000 p55 subunit of TFIIA is isolated form a strain of 410
E coli that contains the coding sequence of human
p55 of TFIIA under the control of T7 promoter(3).
TP200024 NM_004492 TFIIA (Transription Factor IIA, reconstituted) 10000 p55 subunit of TFIIA is isolated form a strain of E coli 205
that contains the coding sequence of human
p55 of TFIIA under the control of T7 promoter (3).
p12 subunit of TFIIA is isolated from a strain of E coli
that contains the coding sequence for human
TFIIA g subunit under the control of T7 promoter.
TFIIA was reconstituted as described in (5).
TP200025 NM_004492 TFIIA (Transription Factor IIA, reconstituted) 25000 p55 subunit of TFIIA is isolated form a strain of E coli 410
that contains the coding sequence of human
p55 of TFIIA under the control of T7 promoter (3).
p12 subunit of TFIIA is isolated from a strain of E coli
that contains the coding sequence for human
TFIIA g subunit under the control of T7 promoter.
TFIIA was reconstituted as described in (5).
TP200026 NM_007296 BRCA1, Tumor Suppressor Protein 2000 The wild type BRCA1 (1-1863 residues) is expressed in 310
and Transcription Factor baculovirus system and purified by an affinity column
in combination with FPLC chromatography.
TP200027 NM_007296 BRCA1, Tumor Suppressor Protein 5000 The wild type BRCA1 (1-1863 residues) is expressed in 620
and Transcription Factor baculovirus system and purified by an affinity column
in combination with FPLC chromatography.
TP200028 NM_007121 LXR- beta (Liver-X Receptor, beta isoform) 10000 Recombinant LXR is isolated from an E coli strain that 204
carries the coding sequence of the human LXR beta
under the control of a T7 promoter.
TP200029 NM_007121 LXR- beta (Liver-X Receptor, beta isoform) 25000 Recombinant LXR is isolated from an E coli strain that 407
carries the coding sequence of the human LXR beta
under the control of a T7 promoter.
TP200030 NM_006924 ASF/SF2, (SFRS1 or SRp30a from E Coli) 10000 The human ASF/SF2 wild type protein (residue1-248) 215
Pre-mRNA Splicing Factor was expressed in E Coli and purified by an affinity
column.
Product offering is subject to change. See our website for the most updated information.
Mis
c.m
ole
cula
rto
ols
Prot
ein
colle
ctio
ns
137ph: 1.888.267.4436 fax: 1.301.340.9254
Cat. # Accn Description Unit Source Price*
TP200031 NM_006924 ASF/SF2, (SFRS1 or SRp30a from E Coli) 25000 The human ASF/SF2 wild type protein (residue1-248) 429
Pre-mRNA Splicing Factor was expressed in E Coli and purified by an affinity
column.
TP200032 NM_006924 ASF/SF2, (SFRS1 or SRp30a from Baculovirus) 5000 The human ASF/SF2 wild type protein (residue1-248) 310
Pre-mRNA Splicing Factor was expressed in baculovirus system and purified by
an affinity column in combination with FPLC
chromatography.
TP200033 NM_006924 ASF/SF2, (SFRS1 or SRp30a from Baculovirus) 12500 The human ASF/SF2 wild type protein (residue1-248) 620
Pre-mRNA Splicing Factor was expressed in baculovirus system and purified by
an affinity column in combination with FPLC
chromatography.
TP200034 NM_006713 PC4 (Positive Cofactor 4, wild type) 10000 Recombinant PC4 protein (wild type, 127 amino 205
Transcriptional Coactivator acids) is isolated from an E coli strain that carries the
coding sequence of human PC4 under the control of
T7 promoter and purified by conventional
chromatography.
TP200035 NM_006713 PC4 (Positive Cofactor 4, wild type) 25000 Recombinant PC4 protein (wild type, 127 amino 410
Transcriptional Coactivator acids) is isolated from an E coli strain that carries the
coding sequence of human PC4 under the control of
T7 promoter and purified by conventional
chromatography.
TP200036 NM_006713 PC4-mt (Positive Cofactor 4, F77P mutant) 10000 Recombinant PC4 protein (mutant F77P, 127 amino 205
Transcriptional Coactivator acids) is isolated from an E coli strain that carries the
coding sequence of human PC4 under the control of
T7 promoter and purified by conventional
chromatography.
TP200037 NM_006713 PC4-mt (Positive Cofactor 4, F77P mutant) 25000 Recombinant PC4 protein (mutant F77P, 127 amino 410
Transcriptional Coactivator acids) is isolated from an E coli strain that carries the
coding sequence of human PC4 under the control of
T7 promoter and purified by conventional
chromatography.
TP200038 NM_006713 PC4-mt (Positive Cofactor 4, serine mutations) 10000 Recombinant PC4 protein (serine mutations, 127 205
Transcriptional Coactivator amino acids) is isolated from an E coli strain that
carries the coding sequence of human PC4 under the
control of T7 promoter and purified by conventional
chromatography.
TP200039 NM_006713 PC4-mt (Positive Cofactor 4, serine mutations) 25000 Recombinant PC4 protein (serine mutations, 127 410
Transcriptional Coactivator amino acids) is isolated from an E coli strain that
carries the coding sequence of human PC4 under the
control of T7 promoter and purified by conventional
chromatography.
TP200040 NM_006233 RNA pol II-hRPB9 (RNA polymerase II, 10000 Recombinant p14.5 is isolated from an E coli strain 205
p14.5 subunit) that carries the coding sequence of human RPB9
under the control of a T7 promoter.
TP200041 NM_006233 RNA pol II-hRPB9 (RNA polymerase II, 25000 Recombinant p14.5 is isolated from an E coli strain 410
p14.5 subunit) that carries the coding sequence of human RPB9
under the control of a T7 promoter.
TP200042 NM_005693 LXR- alpha (Liver-X Receptor, alpha isoform) 10000 Recombinant LXR is isolated from an E coli strain 204
that carries the coding sequence of the human
LXR alpha under the control of a T7 promoter.
TP200043 NM_005693 LXR- alpha (Liver-X Receptor, alpha isoform) 25000 Recombinant LXR is isolated from an E coli strain 407
that carries the coding sequence of the human LXR
alpha under the control of a T7 promoter.
TP200044 NM_005693 LXR-LBD (Liver-X Receptor, ligand binding 10000 Recombinant LXR-LBD is isolated from an E coli 195
domain) strain that carries the coding sequence of the human
LXR under the control of a T7 promoter.
TP200045 NM_005693 LXR-LBD (Liver-X Receptor, ligand binding 25000 Recombinant LXR-LBD is isolated from an E coli 389
domain) strain that carries the coding sequence of the human
LXR under the control of a T7 promoter.
Product offering is subject to change. See our website for the most updated information.
Mis
c.m
ole
cula
rto
ols
Prot
ein
colle
ctio
ns
138 www.origene.com
Cat. # Accn Description Unit Source Price*
TP200046 NM_005513 TFIIE alpha, p56 (Transcription Factor IIE, 10000 The recombinant protein is purified from an E coli 205
alpha subunit) strain that contains the coding sequence of human
TFIIE a subunit under the control of T7
promoter (5).
TP200047 NM_005513 TFIIE alpha, p56 (Transcription Factor IIE, 25000 The recombinant protein is purified from an E coli 410
alpha subunit) strain that contains the coding sequence of human
TFIIE a subunit under the control of T7
promoter (5).
TP200048 NM_002095 TFIIE (Transcription Factor IIE, alpha+ 10000 The recombinant proteins are purified from an E coli 205
beta subunits) strain that contains the coding sequence of human
TFIIE a or b subunit under the control of T7 promoter
(5) (cat. #P1005 and #P1006, respectively) and
functional TFIIE is reconstituted as a heterotetramer
by combining both subunits in a 1:1 molar ratio.
TP200049 NM_002095 TFIIE (Transcription Factor IIE, alpha+ 25000 The recombinant proteins are purified from an E coli 410
beta subunits) strain that contains the coding sequence of human
TFIIE a or b subunit under the control of T7 promoter
(5) (cat. #P1005 and #P1006, respectively) and
functional TFIIE is reconstituted as a heterotetramer
by combining both subunits in a 1:1 molar ratio.
TP200050 NM_005316 TFIIH-p62 (Transcription Factor IIH, p62 10000 Recombinant p62 protein is isolated from an E coli 205
subunit) strain that carries the coding sequence of human p62
under the control of a T7 promoter.
TP200051 NM_005316 TFIIH-p62 (Transcription Factor IIH, p62 25000 Recombinant p62 protein is isolated from an E coli 410
subunit) strain that carries the coding sequence of human p62
under the control of a T7 promoter.
TP200052 NM_005036 PPAR alpha (Peroxisome proliferator- 10000 Recombinant PPAR is isolated from an E coli strain 204
activated receptor, alpha isotype) that carries the coding sequence of the human PPAR
alpha under the control of a T7 promoter.
TP200053 NM_005036 PPAR alpha (Peroxisome proliferator- 25000 Recombinant PPAR is isolated from an E coli strain 407
activated receptor, alpha isotype) that carries the coding sequence of the human PPAR
alpha under the control of a T7 promoter.
TP200054 NM_004492 TFIIA-p12 (Transcription Factor IIA, subunit) 10000 p12 subunit of TFIIA is isolated from a strain of E coli 205
that contains the coding sequence for human TFIIA
gamma subunit under the control of T7 promoter.
TP200055 NM_004492 TFIIA-p12 (Transcription Factor IIA, subunit) 25000 p12 subunit of TFIIA is isolated from a strain of E coli 410
that contains the coding sequence for human TFIIA
gamma subunit under the control of T7 promoter.
TP200056 NM_003921 Bcl-10 (B Cell Lymphoma 10) 6000 The wild type human Bcl-10 protein was expressed 268
in baculovirus system and purified by an affinity
column in combination with FPLC chromatography.
TP200057 NM_003921 Bcl-10 (B Cell Lymphoma 10) 15000 The wild type human Bcl-10 protein was expressed in 534
baculovirus system and purified by an affinity column
in combination with FPLC chromatography.
TP200058 NM_003250 TR- alpha1 (Thyroid Hormone Receptor, 5000 The wild type human TR alpha 1 was expressed in 237
alpha1 isoform) baculovirus system and purified by an affinity column
in combination with FPLC chromatography.
TP200059 NM_003250 TR- alpha1 (Thyroid Hormone Receptor, 12500 The wild type human TR alpha 1 was expressed in 473
alpha1 isoform) baculovirus system and purified by an affinity column
in combination with FPLC chromatography.
TP200060 NM_003286 Topo I Human DNA Topoisomerase I (wild type) 5000 The wild type DNA topoisomerase I protein (765 258
amino acids) was expressed in baculovirus system
and purified by using an affinity column and FPLC
chromatography.
TP200061 NM_003286 Topo I Human DNA Topoisomerase I (wild type) 12500 The wild type DNA topoisomerase I protein (765 515
amino acids) was expressed in baculovirus system
and purified by using an affinity column and FPLC
chromatography.
Product offering is subject to change. See our website for the most updated information.
Mis
c.m
ole
cula
rto
ols
Prot
ein
colle
ctio
ns
139ph: 1.888.267.4436 fax: 1.301.340.9254
Cat. # Accn Description Unit Source Price*
TP200062 NM_003286 Topo I (C651-765) Human DNA Topoisomerase I 5000 The C-terminal domain of DNA topoisomerase I 258
(C terminal domain) protein (residues 651-765) was expressed in
baculovirus system and purified by using an affinity
column and FPLC chromatography.
TP200063 NM_003286 Topo I (C651-765) Human DNA Topoisomerase I 12500 The C-terminal domain of DNA topoisomerase I 515
(C terminal domain) protein (residues 651-765) was expressed in
baculovirus system and purified by using an affinity
column and FPLC chromatography.
TP200064 NM_003286 Topo I (197-651) Human DNA Topoisomerase I 5000 The core domain of DNA topoisomerase I protein 258
(Core Domain) (residues 197-651) was expressed in baculovirus
system and purified by using an affinity column and
FPLC chromatography.
TP200065 NM_003286 Topo I (197-651) Human DNA Topoisomerase I 12500 The core domain of DNA topoisomerase I protein 515
(Core Domain) (residues 197-651) was expressed in baculovirus
system and purified by using an affinity column and
FPLC chromatography.
TP200066 NM_003286 Topo I (N1-197) Human DNA Topoisomerase I 5000 The N-terminal domain (NTD) of human DNA 258
(NTD) topoisomerase I protein (residue 1-197) was expressed
in baculovirus system and purified by using an
affinity column and FPLC chromatography.
TP200067 NM_003286 Topo I (N1-197) Human DNA Topoisomerase I 12500 The N-terminal domain (NTD) of human DNA 515
(NTD) topoisomerase I protein (residue 1-197) was expressed
in baculovirus system and purified by using an
affinity column and FPLC chromatography.
TP200068 NM_003286 Topo I (Y723F) Human DNA Topoisomerase I 5000 The mutant Y723F of DNA topoisomerase I protein 258
(mt Y723F) (Y723F) was expressed in baculovirus system and
purified by using an affinity column and FPLC
chromatography.
TP200069 NM_003286 Topo I (Y723F) Human DNA Topoisomerase I 12500 The mutant Y723F of DNA topoisomerase I protein 515
(mt Y723F) (Y723F) was expressed in baculovirus system and
purified by using an affinity column and FPLC
chromatography.
TP200070 NM_003194 TBP (TATA box Binding Protein) 10000 Isolated from an E coli strain that carries the coding 205
sequence for human TBP under the control off T7
promoter (3).
TP200071 NM_003194 TBP (TATA box Binding Protein) 25000 Isolated from an E coli strain that carries the coding 410
sequence for human TBP under the control off T7
promoter (3).
TP200072 NM_003131 c-Fos (proto oncogene) 5000 Recombinant c-fos was expressed in a baculovirus 258
system and purified by an affinity column in
combination with FPLC chromatography
TP200073 NM_003131 c-Fos (proto oncogene) 12500 Recombinant c-fos was expressed in a baculovirus 515
system and purified by an affinity column in
combination with FPLC chromatography
TP200074 NM_003072 BRG1 (Brahma-related Gene 1 Protein, 5000 The wild type human BRG1 was expressed in 310
wild type) baculovirus system and purified by an affinity
column in combination with FPLC chromatography.
TP200075 NM_003072 BRG1 (Brahma-related Gene 1 Protein, 12500 The wild type human BRG1 was expressed in 620
wild type) baculovirus system and purified by an affinity column
in combination with FPLC chromatography.
TP200076 NM_003072 BRG1-mt (Brahma-related Gene 1 Protein, 5000 The mutant human BRG1 (K798R) was expressed in 258
K798R) baculovirus system and purified by an affinity column
in combination with FPLC chromatography.
TP200077 NM_003072 BRG1-mt (Brahma-related Gene 1 Protein, 12500 The mutant human BRG1 (K798R) was expressed in 515
K798R) baculovirus system and purified by an affinity column
in combination with FPLC chromatography.
TP200078 NM_003016 SC35, (SFRS2 or SRp30b) Pre-mRNA 5000 The human SC35 wild type protein was expressed in 258
Splicing Factor baculovirus system and purified by an affinity column
in combination with FPLC chromatography.
Product offering is subject to change. See our website for the most updated information.
Mis
c.m
ole
cula
rto
ols
Prot
ein
colle
ctio
ns
140 www.origene.com
Cat. # Accn Description Unit Source Price*
TP200079 NM_003016 SC35, (SFRS2 or SRp30b) Pre-mRNA 12500 The human SC35 wild type protein was expressed in 515
Splicing Factor baculovirus system and purified by an affinity column
in combination with FPLC chromatography.
TP200080 NM_002957 RXR alpha (Retinoid X receptor protein) 10000 Recombinant RXR is isolated from an E coli strain 205
that carries the coding sequence of the human
protein under the control of a T7 promoter.
TP200081 NM_002957 RXR alpha (Retinoid X receptor protein) 25000 Recombinant RXR is isolated from an E coli strain 410
that carries the coding sequence of the human
protein under the control of a T7 promoter.
TP200082 NM_002957 RXR-LBD (RXR alpha 198-462) 10000 Recombinant RXR-LBD is isolated from an E coli strain 195
that carries the coding sequence of the human RXR-
LBD under the control of a T7 promoter.
TP200083 NM_002957 RXR-LBD (RXR alpha 198-462) 25000 Recombinant RXR-LBD is isolated from an E coli strain 389
that carries the coding sequence of the human RXR-
LBD under the control of a T7 promoter.
TP200084 NM_002875 RAD51 10000 Recombinant RAD51 was expressed in a strain of 205
E coli and purified by an affinity column in
combination with FPLC chromatography.
TP200085 NM_002875 RAD51 25000 Recombinant RAD51 was expressed in a strain of 410
E coli and purified by an affinity column in
combination with FPLC chromatography.
TP200086 NM_002228 c-Jun (proto oncogene) 5000 Recombinant c-jun was expressed in a baculovirus 258
system and purified by an affinity column in
combination with FPLC chromatography
TP200087 NM_002228 c-Jun (proto oncogene) 12500 Recombinant c-jun was expressed in a baculovirus 515
system and purified by an affinity column in
combination with FPLC chromatography
TP200088 NM_002128 HMG1 (High Mobility Group 1 protein) 10000 Recombinant HMG1 is isolated from an E coli strain 195
that carries the coding sequence of the human HMG1
under the control of a T7 promoter.
TP200089 NM_002128 HMG1 (High Mobility Group 1 protein) 25000 Recombinant HMG1 is isolated from an E coli strain 389
that carries the coding sequence of the human HMG1
under the control of a T7 promoter.
TP200090 NM_002128 HMG1 (Native from Calf Thymus) 10000 HMG1 was purified from calf thymus using several 258
steps of conventional and FPLC chromatography.
TP200091 NM_002128 HMG1 (Native from Calf Thymus) 25000 HMG1 was purified from calf thymus using several 515
steps of conventional and FPLC chromatography.
TP200092 NM_002095 TFIIE beta, p34 (Transcription Factor IIE, 10000 The recombinant protein is purified from an E coli 205
beta subunit) strain that contains the coding sequence of human
TFIIE subunit under the control of T7 promoter (4).
TP200093 NM_002095 TFIIE beta, p34 (Transcription Factor IIE, 25000 The recombinant protein is purified from an E coli 410
beta subunit) strain that contains the coding sequence of human
TFIIE subunit under the control of T7 promoter (4).
TP200094 NM_002096 TFIIF, RAP74 (Transcription Factor IIF, 10000 Recombinant RAP74 protein is isolated from an E coli 205
Rap74 subunit) strain that carries the coding sequence of human
RAP74, the subunit of TFIIF under the control of T7
promoter.
TP200095 NM_002096 TFIIF, RAP74 (Transcription Factor IIF, 25000 Recombinant RAP74 protein is isolated from an E coli 410
Rap74 subunit) strain that carries the coding sequence of human
RAP74, the subunit of TFIIF under the control of T7
promoter.
TP200096 NM_002006 FGF-2 (basic fibroblast growth factor) 5000 Recombinant FGF2 was expressed in a baculovirus 237
system and purified by an affinity column in
combination with FPLC chromatography.
TP200097 NM_002006 FGF-2 (basic fibroblast growth factor) 12500 Recombinant FGF2 was expressed in a baculovirus 473
system and purified by an affinity column in
combination with FPLC chromatography.
Product offering is subject to change. See our website for the most updated information.
Mis
c.m
ole
cula
rto
ols
Prot
ein
colle
ctio
ns
141ph: 1.888.267.4436 fax: 1.301.340.9254
Cat. # Accn Description Unit Source Price*
TP200098 NM_001938 Dr1 (NC2 beta 19 kDa) 10000 Recombinant Dr1 is isolated from an E coli strain 195
that carries the coding sequence of the human
Dr1 under the control of a T7 promoter.
TP200099 NM_001938 Dr1 (NC2 beta 19 kDa) 25000 Recombinant Dr1 is isolated from an E coli strain 389
that carries the coding sequence of the human
Dr1 under the control of a T7 promoter.
TP200100 NM_001429 p300 Tumor Suppressor Protein and 4000 The wild type p300 (2414 amino acid residues) was 310
Transcription Factor expressed in baculovirus system and purified by an
affinity column in combination with FPLC
chromatography.
TP200101 NM_001429 p300 Tumor Suppressor Protein and 10000 The wild type p300 (2414 amino acid residues) was 620
Transcription Factor expressed in baculovirus system and purified by an
affinity column in combination with FPLC
chromatography.
TP200102 NM_001429 p300 (C1135-2414) p300 C-terminus 4000 The C-terminus of p300 (residues 1135-2414) was 310
expressed in baculovirus system and purified by an
affinity column in combination with FPLC
chromatography.
TP200103 NM_001429 p300 (C1135-2414) p300 C-terminus 10000 The C-terminus of p300 (residues 1135-2414) was 620
expressed in baculovirus system and purified by an
affinity column in combination with FPLC
chromatography.
TP200104 NM_000964 RAR- alpha (Retinoic Acid Receptor, 5000 Recombinant RAR alpha was expressed in a 270
alpha isoform) baculovirus system and purified by an affinity column
in combination with FPLC chromatography
TP200105 NM_000964 RAR- alpha (Retinoic Acid Receptor, 12500 Recombinant RAR alpha was expressed in a 539
alpha isoform) baculovirus system and purified by an affinity column
in combination with FPLC chromatography
TP200106 NM_000965 RAR- gamma (Retinoic Acid Receptor, 5000 Recombinant RAR gamma was expressed in a 258
gamma isoform) baculovirus system and purified by an affinity column
in combination with FPLC chromatography
TP200107 NM_000965 RAR- gamma (Retinoic Acid Receptor, 12500 Recombinant RAR gamma was expressed in a 539
gamma isoform) baculovirus system and purified by an affinity column
in combination with FPLC chromatography
TP200108 NM_000966 RAR- beta (Retinoic Acid Receptor, 5000 Recombinant RAR beta was expressed in a 270
beta isoform) baculovirus system and purified by an affinity column
in combination with FPLC chromatography
TP200109 NM_000966 RAR- beta (Retinoic Acid Receptor, 12500 Recombinant RAR beta was expressed in a 539
beta isoform) baculovirus system and purified by an affinity column
in combination with FPLC chromatography
TP200110 NM_033222 p75, Transcriptional Coactivator and Lens 10000 Recombinant p75 protein (wild type, 530 amino 258
Epithelial Cell-Derived Growth Factor acids) is isolated from an E coli strain that carries
the coding sequence of human p75 under the control
of T7 promoter and purified by an affinity column in
combination with FPLC chromatography.
TP200111 NM_033222 p75, Transcriptional Coactivator and Lens 25000 Recombinant p75 protein (wild type, 530 amino 515
Epithelial Cell-Derived Growth Factor acids) is isolated from an E coli strain that carries the
coding sequence of human p75 under the control of
T7 promoter and purified by an affinity column in
combination with FPLC chromatography.
TP200112 NM_033222 p75-CTR [C-terminal region of p75 (LEDGF)] 10000 Recombinant p75 CTR is isolated from an E coli strain 258
that carries the C-terminal coding sequence of the
human LEDGF/p75 (amino acid 322-530) under the
control of a T7 promoter.
TP200113 NM_033222 p75-CTR [C-terminal region of p75 (LEDGF)] 25000 Recombinant p75 CTR is isolated from an E coli strain 515
that carries the C-terminal coding sequence of the
human LEDGF/p75 (amino acid 322-530) under the
control of a T7 promoter.
Product offering is subject to change. See our website for the most updated information.
Mis
c.m
ole
cula
rto
ols
Prot
ein
colle
ctio
ns
142 www.origene.com
Cat. # Accn Description Unit Source Price*
TP200114 NM_000546 p53 (wild type) Tumor Suppressor Protein 5000 The wild type p53 (393 amino acids was expressed in 275
and Transcription Factor baculovirus system and purified by an affinity column
in combination with FPLC chromatography.
TP200115 NM_000546 p53 (wild type) Tumor Suppressor Protein 12500 The wild type p53 (393 amino acids was expressed in 550
and Transcription Factor baculovirus system and purified by an affinity column
in combination with FPLC chromatography.
TP200116 NM_000546 p53 (1-363, C-terminal deletion) Tumor 5000 The C-terminus-deleted p53 (amino acid 1-363) was 275
Suppressor Protein and Transcription Factor expressed in baculovirus system and purified by an
affinity column in combination with FPLC
chromatography.
TP200117 NM_000546 p53 (1-363, C-terminal deletion) Tumor 12500 The C-terminus-deleted p53 (amino acid 1-363) was 550
Suppressor Protein and Transcription Factor expressed in baculovirus system and purified by an
affinity column in combination with FPLC
chromatography.
TP200118 NM_000546 p53 (1-342, C-terminal deletion) Tumor 5000 The C-terminus-deleted p53 (amino acid 1-342) was 275
Suppressor Protein and Transcription Factor expressed in baculovirus system and purified by an
affinity column in combination with FPLC
chromatography.
TP200119 NM_000546 p53 (1-342, C-terminal deletion) Tumor 12500 The C-terminus-deleted p53 (amino acid 1-342) was 550
Suppressor Protein and Transcription Factor expressed in baculovirus system and purified by an
affinity column in combination with FPLC
chromatography.
TP200120 NM_000461 TR beta1 (Thyroid Hormone Receptor, 10000 Recombinant TR is isolated from an E coli strain that 205
beta1 isoform) carries the coding sequence of the human TR
alpha1 isoform under the control of a T7 promoter.
TP200121 NM_000461 TR beta1 (Thyroid Hormone Receptor, 25000 Recombinant TR is isolated from an E coli strain that 410
beta1 isoform) carries the coding sequence of the human TR
alpha1 isoform under the control of a T7 promoter.
TP200122 NM_000321 pRB (Retinoblastoma protein), Tumor 5000 The wild type pRB (residue1-928) is expressed in 310
Suppressor Protein, Transcription Factor baculovirus system and purified by an affinity column
and Proliferation Facto in combination with FPLC chromatography.
TP200123 NM_000321 pRB (Retinoblastoma protein), Tumor 12500 The wild type pRB (residue1-928) is expressed in 620
Suppressor Protein, Transcription Factor baculovirus system and purified by an affinity column
and Proliferation Facto in combination with FPLC chromatography.
TP200124 NM_000176 GR (Glucocorticoid Receptor) 5000 Recombinant GR was expressed in a baculovirus 258
system and purified by an affinity column in
combination with FPLC chromatography.
TP200125 NM_000176 GR (Glucocorticoid Receptor) 12500 Recombinant GR was expressed in a baculovirus 515
system and purified by an affinity column in
combination with FPLC chromatography.
TP200126 NM_000125 ER (Estrogen Receptor) 4000 Recombinant ER was expressed in a baculovirus 325
system and purified by an affinity column in
combination with FPLC chromatography
TP200127 NM_000125 ER (Estrogen Receptor) 10000 Recombinant ER was expressed in a baculovirus 649
system and purified by an affinity column in
combination with FPLC chromatography
TP200128 NM_138473 GAL4-Sp1<sup>Q</sup> [GAL4(94) + 4000 Recombinant GAL4-Sp1 is isolated from an E coli 258
Sp1(266-516)] strain that carries the coding sequence of the fused
protein under the control of a T7 promoter.
TP200129 NM_138473 GAL4-Sp1<sup>Q</sup> [GAL4(94) + 10000 Recombinant GAL4-Sp1 is isolated from an E coli 515
Sp1(266-516)] strain that carries the coding sequence of the fused
protein under the control of a T7 promoter.
TP200130 NM_138473 Sp1 GC-box Binding Protein (Natural 2000 Natural Sp1 fraction was purified from HeLa cell 325
fraction purified from HeLa Cells) nuclear extract by using wheat germ agglutinin
affinity chromatography (12).
TP200131 NM_138473 Sp1 GC-box Binding Protein (Natural 5000 Natural Sp1 fraction was purified from HeLa cell 649
fraction purified from HeLa Cells) nuclear extract by using wheat germ agglutinin
affinity chromatography (12).
Product offering is subject to change. See our website for the most updated information.
Mis
c.m
ole
cula
rto
ols
Prot
ein
colle
ctio
ns
143ph: 1.888.267.4436 fax: 1.301.340.9254
Cat. # Accn Description Unit Source Price*
TP200132 NM_138473 Sp1 GC-box Binding Protein (Recombinant 5000 The wild type Sp1 protein (785 amino acids) was 310
protein purified from insect cells) expressed in baculovirus system and purified by
using an affinity column and FPLC chromatography.
TP200133 NM_138473 Sp1 GC-box Binding Protein (Recombinant 12500 The wild type Sp1 protein (785 amino acids) was 620
protein purified from insect cells) expressed in baculovirus system and purified by
using an affinity column and FPLC chromatography.
TP200134 NM_138712 PPAR gamma (Peroxisome proliferator- 10000 Recombinant PPAR is isolated from an E coli strain 204
activated receptor, gamma isotype) that carries the coding sequence of the human
PPAR gamma under the control of a T7 promoter.
TP200135 NM_138712 PPAR gamma (Peroxisome proliferator- 25000 Recombinant PPAR is isolated from an E coli strain 407
activated receptor, gamma isotype) that carries the coding sequence of the human
PPAR gamma under the control of a T7 promoter.
Product offering is subject to change. See our website for the most updated information.
Mis
c.m
ole
cula
rto
ols
Quan
ti-La
dder
144 www.origene.com
Quanti-Ladder™ DNA Marker
The Quanti-Ladder™ DNA Marker is a ready-to-use
molecular weight marker, ideal for easy quan-
tification and molecular weight determination. The
size standards range from 200 to 10,000 base pairs of
double-stranded DNA.
Product Description
Quanti-Ladder™ DNA Marker consists of bands of
0.2, 0.4, 0.6, 0.8, 1.0, 1.5, 2.0, 2.5, 3.0, 4.0, 5.0, 6.0, 8.0
and 10 kilobases. The 1.0 and 10 kilobase-pairs bands
have a higher intensity than the others to allow quick
and easy identification. Using a standard loading of
5 µL, each band corresponds to an exact quantitation
of DNA. This allows for easy quantification of any
DNA sample.
Quanti-Ladder™ DNA Marker is prepared in a load-
ing buffer containing the tracking dyes bromophenol
blue and xylene cyanol FF and is available in two
formats:
• 1 mL or 200 lanes equivalent
• 5 mL or 1000 lanes equivalent
SIZE (kbp) ng/band
10 100
8.0 80
6.0 60
5.0 50
4.0 40
3.0 30
2.5 25
2.0 20
1.5 15
1.0 100
0.8 80
0.6 60
0.4 40
0.2 20
Product Listing Cat. No. Price
Quanti-Ladder™ DNA Markers, 200 lanes QLD-200 $150
Quanti-Ladder™ DNA Markers, 1000 lanes QLD-1000 $595
ADVANTAGES
Ideal for easy quantificationof DNA samples
14 DNA bands for accuratesize determination
Easy to remember sizesranging from 0.2 to 10 kb
Mis
c.m
ole
cula
rto
ols
Mill
enni
um™
mar
ker
145ph: 1.888.267.4436 fax: 1.301.340.9254
Millennium™ RNA Marker
The Millennium™ RNA Marker has been developed
to provide easy and accurate molecular weight
determination of single-stranded RNAs from 0.5 to
9.0 kb, and is ideal for Northern blot protocols.
Product Description
The RNA ladder consists of a series of transcripts of
0.5, 1.0, 1.5, 2.0, 2.5, 3.0, 4.0, 5.0, 6.0 and 9.0
kilobases. This large number of well-separated RNA
bands allows the construction of a very accurate
standard curve for determining the sizes of unknown
mRNA species. Each lot contains bands at
approximately the same intensity and is free of
nuclease contamination.
The Millennium™ RNA Marker is provided at a
concentration of 1 µg/µL. The bands are easily
viewed by ethidium bromide staining when 2 µL are
separated in a denaturing agarose gel.
ADVANTAGES
Ten RNA bands foraccurate size determination
Easy-to-remember sizesranging from 0.5 to 9.0 kb
Markers hybridize with λ DNA forautoradiographic detection
Product Listing Cat. No. Price
Millennium™ RNA Markers, 25 lanes RMM-3703 $105
9
6
5
4
3
2.5
1.5
0.5
1
2
size (kb)
Mis
c.m
ole
cula
rto
ols
Rapi
d-Lo
ad
146 www.origene.com
Rapid-Load™ PCR Loading Dye
Rapid-Load™ is a 5x sample loading dye that is
added to a PCR mix before amplification. The
presence of Rapid-Load™ in the PCR mix will not
interfere with the performance of the overall reaction.
After the PCR is finished, the reaction products can be
directly loaded in an agarose gel. It is not necessary
to add standard loading dye to the samples. It
eliminates the need for an extra pipetting step.
Product Description
Rapid-Load™ is red in color making it easy to load
samples and track them in an agarose gel during
electrophoresis. The red dye migrates at 1.4 kbp in a
1% agarose gel with 1x TAE running buffer.
Rapid-Load™ can be used in PCR amplifications
containing standard plasmid DNA, genomic DNA or
bacterial colonies. It is also ideal for use with
multiple samples such as when performing a 96-well
PCR.
Rapid-Load™ is available in two formats:
• 5 mL or 500 PCR equivalent
• 25 mL or 2500 PCR equivalent
Fig. 1 shows PCR amplification of human b-actin from
plasmid DNA, transformed bacterial colonies or geno-
mic DNA. Each reaction was performed in duplicate in
the presence (+) or absence (-) of Rapid-Load™.
– +– +– +
plasmidDNA
bacterialcolonies
genomicDNA
kb
1.0 -0.6 -
Fig.1
ADVANTAGES
Saves time
Easy to use
Ideal for 96-well PCR
Product Listing Cat. No. Price
Rapid-Load™ PCR Loading Dye, 5 mL RL-105 $25
Rapid-Load™ PCR Loading Dye, 25 mL RL-125 $100
Ord
erin
gin
form
atio
n
147ph: 1.888.267.4436 fax: 1.301.340.9254
To Order
E-Commerce: www.origene.com
Telephone: 1-888-267-4436 (toll-free in U.S. only)
1-301-340-3188 (9AM – 5PM EST, M – F)
Fax: 1-301-340-9254 (24 hrs)
E-mail: custsupport@origene.com
Address: 6 Taft Court, Suite 100
Rockville, MD 20850
Please include the following information when ordering via fax:
1. Name and telephone number of person placing order
2. Catalog number, description, and quantity of item ordered
3. Purchase order number or credit card* information
4. Shipping address
5. Billing address
*For your convenience, we accept VISA®, MasterCard® and AmericanExpress®. To order using a credit card,
please supply the cardholder name (as it appears on the card), the account number, and the expiration date.
By placing an order with OriGene, you agree to the following:
PRICINGPrices are subject to change without notice. For current pricing information on any product, please contact
OriGene Customer Service.
PAYMENT TERMSNet 30 days from the date of invoice in U.S. dollars.
PARTIAL SHIPMENTAll orders are normally filled within a single shipment. If this is not possible, a partial shipment will be sent
unless otherwise indicated when the order is placed.
RETURNS AND CREDITSOriGene products may not be returned without prior authorization from Customer Service (1-301-340-3188).
For returns due to damaged products or missing components, please obtain an authorization number and
instructions for returning the goods to the Customer Service Department. The products must be returned in
their original, intact condition to receive credit. In the event of returns due to customer ordering error, there
will be a 20% restocking fee and shipping charges will not be credited.
CONDITIONSAll OriGene products are for research use only and are not intended for drug, diagnostic, or human use. The
resale, modification or duplication for resale, or any other transfer for value of intact, duplicated or modified
product, is strictly prohibited. OriGene products may be protected by patents, pending patent applications,
and/or copyrights.
Ord
erin
gin
form
atio
n
148 www.origene.com
SHIPPINGProducts are shipped F.O.B. Rockville, Maryland. Shipping charges are prepaid and added to your invoice.
Domestic orders requiring shipment on dry ice are delivered via Federal Express Priority Overnight Service,
Monday through Thursday, unless otherwise requested.
CUSTOM AND BULK ORDERSPlease request quotations for all custom and bulk orders by calling OriGene at 1-301-340-3188 or 1-888-267-4436
(toll-free in U.S. only).
LIMITED PRODUCT WARRANTYOriGene Technologies, Inc. warrants that the product will meet specifications listed. At OriGene’s discretion,
free replacement of any nonconforming product will be made if OriGene is notified within 30 days of product
receipt. This warranty limits OriGene’s liability to the cost of replacement of the product in question only.
OriGene provides no other warranty, express or implied, and is not liable or responsible for any indirect or
incidental damages or loss as a consequence of product use. No warranty is given for products used after the
printed expiration date or for products not stored or used according to the product use specifications given. If
you experience difficulty with any OriGene product, please contact OriGene Technical Support promptly at
1-301-340-3188, ext. 2 or 1-888-267-4436, ext. 2.
PCR is covered by patents issued to Cetus Corporation and owned and licensed by Hoffmann-La Roche, Inc. Purchase of any of OriGene’s PCR-related products does not convey a licenseto use the PCR process covered by these patents. Purchasers of these products must obtain a license to use the PCR process before performing PCR.
U.S. Postage
PAID
6Taft Court, Suite 100
Rockville, MD 20850
top related