Studies on cycloferon by VICTORIA, RUBEN GOMBALANDI, IMMUNOMODULATORS

CYCLOFERON INDUCED CHANGES IN STOMACH OF OLD RATS

REPORT PRESENTED BY RUBEN GOMBALANDI,HEADED BY MISS VICTORIA

MINISTRY OF HEALTH UKRIANE LUGANSK STATE MEDICAL UNIVERSITYDEPARTMENT OF BIOLOGICAL SCIENCE

INTRODUCTION

• BOTH Interferon and inductor of endogenous production of interferon have gained clinical acceptance in recent years. WHY?

• They have antiviral and immunomodulatory property• They have no side effect• They can be use for long term therapy.

immunomodulators

IMMUNE MODIFIERS

Immunosuppresants

Immune tolerance

Immunostimulants

LevamisoleThalidomideBCGRecombinant Cytokines

InterferonsCyclopheron*

IMMUSTIMULATORS

CyclopheronAntiviralImmunomodulatory activityBind to cell surface receptors – initiate intracellular eventsEnzyme inductionInhibition of cell proliferationEnhancement of immune activitiesIncreased Phagocytosis

Uses : HSV infection; influenza and acute respiratory viral diseases; Chronic viral hepatitis B and C; Acute intestinal infections; secondary immunodeficiency associated with chronic bacterial and fungal infections.

Scheme of experiment:Age Group kind of

influenceDuration of the experiment in days and the number of animals

old rats (mass of 330 - 350 gr.)

7 day 15 days

30 days

90 days

180 days

1 Cyclopheron

6 6 6 6 6

2 Control of cyclopheron

6 6 6 6 6

Total 60

What Was use for the study

LIGHT MICROSCOPY and Morphometric methood was used for the study.

FINDINGSCONTROL group• Normal• Normal

EXPERIMENTAL GROUP• Rats killed 7 days after

injection,number of chief cell droped by 13% compared to the Contro group.

• Area of zymogenic cells and their nucleus was less by 12% and 15% compare to the control group

Common Findings

IN BOTH GROUPS THE AVERAGE NUMBER OF PARIETAL CELLS AND THE AREA OF THEIR NUCLEUS WAS THE SAME.

RESULT OF CYCLOFERONE ON OLD RATS AFTER 7 DAYS

7дн. 15дн. 30дн. 90дн. 180дн.0

10

20

30

40

50

60

*

**

*

*

*

*

ГК,ЦФ ГК,КЦФ ПК,ЦФ ПК,КЦФ

DAY 15AND 30

At Day 15, and 30 changes in the number and morphometric parameters of the chief and parietal cells were not revealed.

Result on the 15th Day

BY DAY 90

By the 90th Day, Parietal cells showed an increase in number by 42%, but morphometric data did not show any increase in functional activity.

At the End of the experiment

Parietal cells inceased by 21% and the chief cells increased by 28%.But there was not difference in data of area and diameter of cell between experimental and control group.

SYNOPSIS LEADING TO OUR CONCLUSION

Ageing leads to decrease response of the cells to cytokines.,also gene expression of the IL-2 descrease with ageing of the immune system. But exogenous IL-2 restores the ability of CD4+ to respond by proliferation through the Transforming growth factor. TGF alpha,play a role in the regulation of acid secretion and mucosal renewal in the stomach of old rats.therefore we suggest t

IN CONCLUSION

We suggest that cycloferon activate T- cells (CD4 + lymphocytes) that produce IL- 2 which via the TGF-α expressed by stomach chief and parietal cells mediate proliferation of secretory cells to the end of experiment.