Structure and composition of nuclear pore complexes (NPCs)

Structure and composition of nuclear pore complexes (NPCs)

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Electron micrographs of NPCs

Gall, 1967

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Gall, 1967

Electron micrographs of NPCs

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Three-dimensional view of a nuclear pore in the double-layered nuclear envelope

Gall, 1967

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Diagram of a model cylinder with the x-, y- and z-axes

Akey & Radermacher, 1993

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Akey & Radermacher, 1993

Averaged xy slices taken from the 3D map of the NPC

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Akey & Radermacher, 1993

3D side view of the NPC surface map

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z slices taken from 3D maps of the NPCs

Akey & Radermacher, 1993

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Akey & Radermacher, 1993

Surface views of the NPC 3D map

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3D representation of the membrane-associated NPC

Akey & Radermacher, 1993

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3D structure of the NPC

Beck et al, 2004

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Cutaway view of the NPC

Beck et al, 2004

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Slices through the structure of the pore

Beck et al, 2004

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The NPC nuclear basket

Kiseleva et al, 2004

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The NPC cytoplasmic particles

Kiseleva et al, 2004

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The NPC cytoplasmic particles

Kiseleva et al, 2004

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Immuno-labelling of Nup116

Kiseleva et al, 2004

The NPC is constructed from three main parts: 1) cytoplasmic components 2) central parts3) nucleoplasmic components

The NPC has eight rotational symmetry.

The NPC is huge structure. The vertebrate NPC has about 125 nm diameter (around 20 times larger than a ribosome).

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Comparison of the peripheral and intrinsic membrane polypeptides of nuclear envelopes and microsomal

membranes

Gerace et al, 1982

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Characterization of antibodies to gp 190 and the lamins

Gerace et al, 1982

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Immunofluorescence staining of tissue culture cells with anti-gp 190 and anti-lamins A and C

Gerace et al, 1982

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Immuno-labeling of nuclei with anti-gp 190

Gerace et al, 1982

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Presence of gp 190 in diverse vertebrate species

Gerace et al, 1982

Mammalian, yeast and worm nucleoporins

Angelo & Hetzer, 2008 (Review)

Like structure, the composition of NPC is highly conserved between human and yeast.

The NPC is composed of ONLY about 30 different proteins (nucleoporins). Ribosome has 80 different protein components.

Schematic illustration of the NPC structure

Angelo & Hetzer, 2008 (Review)

Predicted composition of scaffold and central channel

Angelo & Hetzer, 2008 (Review)

Predicted composition of cytoplasmic components

Angelo & Hetzer, 2008 (Review)

Predicted composition of nuclear components

Angelo & Hetzer, 2008 (Review)

Predicted composition of transmembrane components

Angelo & Hetzer, 2008 (Review)

Predicted localization of sub-complexes and nucleoporins within the NPC

Angelo & Hetzer, 2008 (Review)

The NPC sub-complexes are divided to two categories:1) peripheral 2) central

The nuclear pore complex in turn is made of several protein sub-complexes.