Transcript
Barcoding the Kelp Forests of
California’s Channel Islands National Park
Ralph Imondi, David Kushner, Daniel Distel, Timery Deboer, Robert Hanner,
and Linda Santschi
Rattus norvegicus Mus musculus Gallus domesticus
Danio rerio Drosophila melanogaster
Schistocerca nitens
Xenopus laevis
Caenorhabditis elegans Hirudo medicinalis
Energizing science through
interdisciplinary studies
concepts
tools processes
ecology
environmental
science & policy
molecular
biology
genetics
biochemistry
earth science
computer
science
cell biology developmental
biology
Mending the disconnect between
scientific research and science education
scientific research community 2˚ educational community
exploration
& discovery
community
analysis
&
feedback
benefits
&
outcomes
TESTING
IDEAS
Emphasizing the scientific discovery process
utilizing barcode data
application
user
research
user
general
public
GenBank BOLD
EOL
Potential student entry points into the
DNA barcoding workflow
specimen collecting and
tissue sampling
Student engagement in the entire workflow
specimen collecting
tissue processing
creating specimen pages on BOLD
generating COI amplicons
analyzing, editing, and uploading sequence data
California’s northern Channel Islands
The northern Channel Islands are fringed by
extensive kelp forest habitat
Upwelling features near the islands
contribute to kelp forest productivity
* * *
*
Point
Conception
Ventura
Santa Barbara
Los Angeles
California
Current
California
Countercurrent
cold subartic
water
warm equatorial
Pacific water
San Luis Obispo
Oregonian
Province
Californian
Province
The confluence of major current systems
gives rise to ecologically diverse species
assemblages
Santa Cruz Anacapa San Miguel Santa Rosa
variable transition zone
special
assemblage
temperate
biota
boreal
biota
Pycnopodia helianthoides Hypsypops rubicundus
The CINP monitors up to 37 sites around the
northern Channel Islands
San Miguel
Santa Rosa Santa Cruz
Anacapa
the sites reflect the broad range of conditions and
biological assemblages found in the park
O. pictus C. gigantea P. helianthoides C. punctipinnis
B. elegans H. rufescens M. californica S. californicus
U. lofotensis H. rubicundus M. undosa O. californica
The park collects abundance data for select
species of algae, invertebrates, and fishes
C. californica C. spadicea P. miniata C. nicholsii
P. stearnsii K. kelletii P. californicus E. jacksoni
L. chilensis M. crenulata D. imbricata L. dalli
Sebastes barcoding project
S. mystinus S. chrysomelas S. auriculatus S. rosaceus
S. dalli S. carnatus S. semicinctus S. serriceps
S. umbrosus S. atrovirens S. miniatus S. serranoides
number of species
number
of species
1 - 5
6 - 10
11 - 15
16 - 20
21 - 25
26 - 30
31 - 35
36 - 40
41 - 45
46 - 50
51 - 55
56 - 60
1960 1965 1970 1975 1980 1985 1990 1995 2000 2005 2010
1.0
0.8
0.6
0.4
0.2
0
rela
tive d
eple
tion
Rockfishes are a heavily exploited by
commercial and recreational fisheries
Pacific Ocean Perch
Widow
Yelloweye
Bocaccio
Darkblotched
Cowcod
Canary
The inability to identify larval rockfish
hinders efforts to characterize their
distribution, abundance and life history traits
Forms of student engagement
Direct (residential program for students)
Indirect (professional development program for teachers)
CMB
training
instructional materials
standardized lab protocols
transportable equipment
CMB
high
school
Collaboration framework (scientific strand)
specimen
collection & tissue
processing sample curation
& data accession
Ocean
Genome
Legacy taxonomic
verification
& vouchering
SBMNH
CCDB distribution
students
CINP resource management & enforcement personnel
UCSB Marine Operations Facility
volunteers
Economic workforce development linkages
Recruitment Equipment support
Residential program for students
Professional development program
CMB
high
schools
SoCal Biotech Center
LA/OC Biotech Center
North Valley Biotech Center
Bay Area Biotech Center
Recruitment
Equipment support
Economic workforce development linkages
Arcadia
Bakersfield
Calabasas
Camarillo
Carpinteria
Chatsworth
Compton
Dana Point
Dos Pueblos
Dublin
Fontana
Granada Hills
Hollywood
Huntington Beach
La Jolla
Lakewood
Long Beach
Los Angeles
Los Altos
Los Gatos
Los Osos
Mission Viejo
Moorpark
North Hollywood
Norwalk
Oak Park
Geographic Distribution of Participants
300 km
Project status
NORTHERN CHANNEL ISLANDS SEBASTES BARCODE PROJECT [CMBS]
Project status
Channel Islands Kelp Forest and MPA Fish and Invertebrates [CNOI]
Geographic Distribution of Participants
300 km
Mohave
Desert
Expanding student access to specimens
Sonoran
Desert
Klamath
Sierra
Nevada San
Francisco
Bay Area
Mediterranean
Coast
Alignment of student-led barcoding activities
with NPS I&M activities and youth programs
Technology Resource Development
Technology Resource Development
www.DNAbarcodingAssistant.org
Technology Resource Development
CMB Guelph
BOLD data portal
meeting the
barcode data
standard
user user
BOLD
Technology Resource Development
high
schools
Guelph
BOLD
data portal
meeting the
barcode data
standard
professional
user
BOLD
professional
user
non-professional
user
pre-BOLD
Biotechnology ExplorerTM
An Internet platform to broaden student
engagement in DNA barcoding and iBOL
Robert Hanner
Sujeevan Ratnasingham
Acknowledgements
David Kushner
Sonia Ibarra
Eric Mooney
Joshua Sprague
Milton Love
Samuel Hammond
Christoph Pierre
Dan Distel
Abigail Fusaro
Timery Deboer
Eric Hochberg
Paul Valentich-Scott
Jeffery O’Neal
Wendi Johnston
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