Protein protein interaction basic
Post on 11-Nov-2014
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Groups members
Ayesha Aftab Malik Amber Malik
What is a Protein?
Proteins are the workhorses that facilitate most biological processes in a cell.
Critical aspects required to understand the function of a protein
Expression profile
Post-translational modifications
Intracellular localization
Interactions with other proteins
Evolutionary history and conserved sequence
Protein sequence and structure
PROTEIN PROTEIN INTERACTION
Need of protein protein interaction
The majority of proteins interact with other proteins for proper function
Publication of the human genome Development of the field of
proteomics,
TYPES OF PROTEIN PROTEIN INTERACTION
Two types Stable Transient Both types can be weak or strong
Biological effect of protein protein interaction
Alter the kinetic properties of enzymes Allow for substrate channeling Create a new binding site Inactivate or destroy a protein Change the specificity of a protein for
interaction with different proteins Serve a regulatory role
Common Methods to Analyze Protein-Protein Interactions In Wet Lab
Co-ImmunoprecipetationPull-down assay
Cross linking protein interaction analysisLabel transfer protein interaction analysis
Far-western blot analysis
DEFECTS IN WET LAB PROCESSES Slow Time consumingNot accurateDifficult to handle CostlyLack of equipments
Common methods of PIP’s in BioinformaticsPrimary databasesInt actMintBindstring
Meta databasesPINAAPIP
DATABASES
STRING: known and predicted protein interactions.
Interactions physical and functional associations
The database currently covers 5'214'234 proteins from 1133 organisms.
DATABASES
K VOTE to create seven different integrated
networks by using values of k ranging from 1-7.
modularity, similarity-based modularity, clustering score, enrichment.
Detection and quantification of protein interactions
Databases of protein-protein interactions and their use in drug discovery.
Proteins rarely act alone
Case of use of PPI’s
Questions
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