Neuron cell under strong laser beam

Neuron cell under strong laser beam

江宏仁 2003.07.07

Living soft matter ?

NEURONAL MORPHOGENESIS ANDSTRUCTURAL PLASTICITY:

Axon Initiation Axon GrowthAxon Guidance Axon Branching Dendritic Growth, Guidance, and Branching Dendritic Spine Formation, Stability, and Rapid MotilitySynapse Formation and Stability Axon and Dendrite Retraction

PHENOMENOLOGY

Growrh cone

protrusion

anchorage

forward movement

tail retraction

4 processes coordinated:

Axon Guidance

Laser:1064nm 700mW source 50 mW after nosepiece

Guiding neuronal growth with light ?

Optical forces can impact diffusive behavior.

Theory 1

Mechanical manipulation of a membrane.

Theory 2

Dynamic Excitations in Membranes Induced by Optical Tweezers

Colloidal effects

Theory 3

PEARLING OF CYLINDRICAL VESICLES

Laser-induced rounding

Tests for each theory

Theory 1 :* in vitro actin polymeried under laser beam * Listeria movement as membrance moving model

Theory 2: *using flow cell to apply shear force on membrance

Theory 3: *using indirect phenomena ex: pearling, rounding, retrcation, or in water *direct observe by EM or light scattering