Transcript
8/18/2019 Nanotech Retina
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NANOTECHNOLOGY
APPLIED TO THE RETINA
David Amat
16 de novembre de 2015Curs 2015-2016Nanotecnologia
Enginyeria Fsica
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REFERENCES
!asic "eference
• Intravitreal silicon-based quantum dots as neuroprotective factors in amodel of photoreceptor degeneration (Olson JL, Velez-Montoa !,Mandava ", #toldt $!% IOV# Jorunal &'&, )ug
#t$er "eferences
• "europrotective *+ect of hotoactive uantum .ots in rogressive!etinal hotoreceptor .egeneration (Olson JL, Velez-Montoa !, Mandava", #toldt $!% Journal of "anomaterials / Molecular "anotechnolog, &'0
Jul
• )rti1cial means for restoring vision (ar2ez 3ossain, Ian 4 #eetho,
)ndre2 $ 5ro2ning,4infried M )moa6u%, 5MJ Journal, &''7 8eb
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OUTLINE
• %ntroduction to retinal structure and biological be$aviour
• Aim of t$e e&'eriment
• Designing t$e e&'eriment
• "esults
• Discussions
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Introduction to retinal
structure
161 million people with visal impairment !2"2# o$ worl%wi%e poplation&
'etinal %isor%ers( %egeneration o$ neral cells !photoreceptors" )ipolar cells*& +rreversi)le e$$ect Electric stimlation as metho% o$ prevention
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Bioloical !e"a#iour
• Dis(s in rods contain r$odo'sin 'roteins
• "$odo'sin 'roteins contain 11-cis retinal
• )$otoisomeri*ation becuase of lig$t
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Bioloical !e"a#iour
• +$en lig$t stri(es, t$e transducin 'rotein is AC%.AED
• )DE transforms c/) to /)
• Closure of ion c$annels
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Bioloical !e"a#iour
• Absence of c/) ma(es t$e ion c$annels to C#E
• Negative 'otential inside
• "ods $y'er'olari*ed
• Electric negative current send to !%)#A" CE
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Ho$ e%es are tested &ERG'
• a-3ave due to '$otorece'tor negative 'otential res'onse
• b-3ave due to bi'olar and ganglion cells
• "od res'onse in DA"4 ADA)ED EE
• Cone res'onse in %/ ADA)ED EE
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Ai( o) t"e e*+eri(ent
hotoreceptor %egeneration
starts at 3r% wee o$ li$e
se o$ antm %ots as electrical stimlos generator
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Desinin t"e e*+eri(ent
32 eyes divided in 4 groups of 8 eyes each
ontrol ham 7 active 7 inactive
1 dose 2 doses 3 doses
4 eyes 2 eyes 2 eyes
• $am grou' received saline in7ection
• 8D in a colloidal a9ueous medium con7ugated 3it$ surfactant 'roteins
• 8D active: silicon 8D, gets e&cited by ;50 nm 3avelengt$ , and t$ird dose at 3ee( 6
• After ; 3ee(s, rats eut$ani*ed and $istological retina images analysis
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Results
,- Rod res+onse !% rou+s
• ig$ voltage res'onse in t$e active 8D?s eyes
analy*ed
• %nactive 8D?s eyes be$ave as t$e s$am grou'
• %nactive, s$am and control grou' s$o3 a 'oor
voltage res'onse at 3ee( ; com'ared to active 8D
$ig$ res'onse
ilicon
8ol%
ham
ontrol
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Results
.- /uantu( Dots (iration
• A: one $our after t$e in7ection 9uantum dots internal membrane retina
• !: i& $ours after t$e in7ection 9uantum dots migration across retina
• !y 2 day all 8D $ave crossed retina
• Nano'articles in7ected in eye are cleared by endot$elial system
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Results
0- Nu(!er o) in1ections
• "e'etitive 'rocess im'roving t$e results
1 in9ection
2 in9ections
3 in9ections ontrol
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Results
2- Retina "istolo%3 cell count
• A: ilicon 8D?s eye: all reinal layers 3ell-'reserved
• !: /old 8D?s eye: loose of '$otorece'tors in #N
• C: Control eye: very t$in layer
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Discussions
Electric
stimulation
%m'lants
C$i' retina
Dra3bac(s
)ar(inson
urgery
needed
"etinal
detac$ment
8uantum
Dots
No surgery
ore @8D
)rocess
re'eated
' remove
coating
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T"an4s )or %our attention
No$ it5s %our turn3
I) %ou "a#e an%
/ U E S T I O
N 1ust AS6
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