microarray DNA - Kursused - Arvutiteaduse instituut · Presentation outline 1. What is DNA microarray 2. DNA microarray usage 3. How does it work 4. Analyzing of results

DNAmicroarrayPavlo Tertychnyi6March2017

Presentationoutline

1. WhatisDNAmicroarray2. DNAmicroarrayusage3. Howdoesitwork4. Analyzingofresults

Presentationoutline

1. WhatisDNAmicroarray2. DNAmicroarrayusage3. Howdoesitwork4. Analyzingofresults

WhatisDNAmicroarray

WhatisDNAmicroarray

Presentationoutline

1. WhatisDNAmicroarray2. DNAmicroarrayusage3. Howdoesitwork4. Analyzingofresults

Medicalfield

Helpresearcherslearnmoreaboutdiseases:1. Whatcausesdiseases2. Howtoidentifydiseases3. Howtotreatthem

Multiplesclerosis

Foodproduction

Foodproduction

Please,don’teatme…

Presentationoutline

1. WhatisDNAmicroarray2. DNAmicroarrayusage3. Howdoesitwork4. Analyzingofresults

Howdoesitwork

Howdoesitwork

Howdoesitwork

Example

Presentationoutline

1. WhatisDNAmicroarray2. DNAmicroarrayusage3. Howdoesitwork4. Analyzingofresults

Analyzingofresults

Analyzingofresults

Analyzingofresults

Analyzingofresults

DNAmicroarraydatabases

Summary

1. Usedtoidentifyspecificgenesandtheactivityofgenes.2. Usedtodeterminehowourgenesareaffectedbyenvironmental

factors,drugsanddrugdosages.3. Wideusedinmedicinefield4. Identifyspecificcharacteristicsinhumanaswellasotherspecies.5. Foodproduction

Coolvisualization

https://www.youtube.com/watch?v=VNsThMNjKhM

Acknowledgement1. http://scme-

nm.org/index.php?option=com_docman&task=cat_view&gid=97&Itemid=225

2. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4011503/pdf/nihms-563981.pdf

3. http://www.ub.edu/stat/docencia/bioinformatica/microarrays/ADM/slides/A_Tutorial_Review_of_Microarray_data_Analysis_17-06-08.pdf

4.http://www.ebi.ac.uk/arrayexpress/5.https://www.ncbi.nlm.nih.gov/geo/6.http://www.hhmi.org/biointeractive/how-analyze-dna-microarray-data

Homework

1.WhatisDNAmicroarray?

a)Specificdatatypeb)Microchipc)SubsequenceofaDNAd)Giftofthegods

Homework

2.Describeatleast3applicationofDNAmicroarray(Imentionedtwoapplicationsduringthepresentation,souseyourimaginationorjustgoogletofindmore).3.DescribetheprocessofpreparationofamaterialbeforepouringitonaDNAmicroarray.

Homework

4.Jointhenextstatements:

a)Therearegenesofahealthycellmainly.b)Therearegenesofasickcellmainly.c)Therearegenesofbothhealthyandsickcells.

1)RedcolorofDNAmicroarrayunit.2)GreencolorofDNAmicroarrayunit.3)YellowcolorofDNAmicroarrayunit.

Sendyourhomeworksolutionshere

pavel.tertychny@gmail.com

Thanks!