MICHAEL HOOKER MICROSCOPY Core Facility—open to all UNC investigators Confocal – fluorescence/reflection/frap/fret Atomic Force Microscope Laser Micro-Dissection.

MICHAEL HOOKER MICROSCOPY

• Core Facility—open to all UNC investigators

• Confocal – fluorescence/reflection/frap/fret• Atomic Force Microscope• Laser Micro-Dissection• Light Microscopy –

tans./fluor./DIC/phase/multimode/time-lapse

http://mhmicroscopy.med.unc.edu

Mike Chua 843-3268

MICROSCOPY SERVICES

• UNC Core facility• Confocal• Laser Capture Microscopy• Electron Microscopy• Light Microscopy

http://www.med.unc.edu/microscopy/Bob Bagnell 966-2413

FLOW CYTOMETRY CORE

• UNC Core Facility

• Deconvolution System (3D)

• Laser Scanning Cytometer

• FACS

http://flowcytometry.med.unc.edu/Larry Arnold 966-1530

R. SUPERFINE - T. SALMON LABS

• Scanning Electron Microscopy• Transmitted Electron Microscopy• Atomic Force Microscopy• 3D Force Microscopy• Confocal Microscopy• Light Microscopy

rsuper@physics.unc.edutsalmon@email.unc.edu

RUSSELL TAYLOR- VISUALIZATION LAB

• Scientific Visualization• Distributed Virtual Worlds• Haptic Display• Interactive 3D Computer Graphics• UNC NIH National Research Resources

for Computer Integrated Systems for Microscopy and Manipulation--http://www.cs.unc.edu/Research/nano/cismm/

UNC NEUROIMAGING LABORATORYG. Gerig, A. Belger, J. Lieberman, J. Piven

• Cognitive Neuroscience– ERP lab: Functional brain mapping– Functional brain mapping using fMRI

• Analysis of MRI diffusion tensor imaging (DTI)• 3D ultrasound: Quantitative image analysis• Developmental Neuroimaging: UNC-NDRC Core• High-power computer system dedicated to neuroimage analysis

Neurolab: http://zeus.ia.unc.edu/NIRL: http://www.nirl.unc.edu/NDRC: http://www.fpg.unc.edu/~ndrc/cores.cfm

BARRY WHITSEL LAB

• Intrinsic Optical Signal Imager (cell-based, in vivo)

bwhitsel@med.unc.edu

BIOLOGY Kerry Bloom – Ted Salmon – Tony Perdue

• Spinning disk Confocals

• Laser Scanning Confocals

• Transmission Electron Microscopy

kerry_bloom@unc.edutsalmon@email.unc.edutdperdue@email.unc.edu

BIOMEDICAL ENGINEERING

• MRS Metabolomics Imaging (J. MacDonald)

• Cortical Intrinsic Signal Imaging

• Multiphoton fluorescence Imaging

• Transmembrane Potential Imaging

• Intracellular Calcium Imaging

• Biophotonics Computer Imaging

macdona1@email.unc.edu

CELL & DEVELOPMENTAL BIOLOGY J. LeMasters – J. Costello – K. Jacobson

• Confocal – Single & 2 Photon• Cryo – Transmitted Electron Microscopy• Scanning Electron Microscopy• Optical Tweezers• Fluorescence Recovery After Photobleaching

lemaster@med.unc.edu

mjc@med.unc.edu

frap@med.unc.edu

CHEMISTRY

• Nuclear Magnetic Resonance

• Spectroscopy

• Total Internal Reflection Microscopy – Nancy Thompson

nit@unc.edu

DENTAL SCHOOL

• Transmitted Electron Microscopy

• Scanning Electron Microscopy

Wallace Ambrose

wallace_ambrose@dentistry.unc.edu

PEDIATRICS/EPA—J. CARSON

• Freeze Fracture

• Transmitted Electron Microscopy

• Scanning Electron Microscopy

• Light Microscopy

jlcarson@email.unc.edu

RADIATION ONCOLOGYEdward Chaney

• CT

• Segmentation

• Registration

• 3D & 4D Display

chaney@med.unc.edu

RADIOLOGY – RESEARCHWeili Lin– Etta Pisano – Stephen Aylward

• 3 Tesla Magnetic Resonance Imaging (Lin)• Micro Single-Photon Computed Tomography

(Lin)• Diffraction Enhanced Imaging(Pisano)• Computer Aided Diagnosis and Display Lab

(CADDLab: Aylward)-- 2D and 3D image segmentation and registration--Multi-modal image resgistration

weili_lin@med.unc.edu; etta@med.unc.edu;aylward@unc.edu

COMPUTER-ASSISTED SURGERY& IMAGING LAB (CASILAB)

Elizabeth Bullitt

• Vessel/Tumor

Imaging and Analysis

• Imaged Guided

Surgery

bullitt@med.unc.edu

GENE THERAPY CENTER

• Optical Imaging (Cryocooled, luminescence and fluorescence)

Allison Hawke

allison_hawke@med.unc.edu

LINEBERGER COMPREHENSIVE CANCER CENTER

• Light Microscopy

• Fluorescence Microscopy

Steve Oglesbee

big_rock@email.unc.edu

NEUROSCIENCES & NEURODEVELOPMENTAL DISORDERS RESEARCH CENTERS

Eva Anton - Robert Sealock

• Confocals – Single & 2-Photon

• Light Microscopy

sealock@med.unc.eduanton@med.unc.edu

VASCULAR BIOLOGY CENTER Cam Patterson

• Ultrasound for small animals

cpatters@med.unc.edu

MEDICAL IMAGE DISPLAY & ANALYSIS GROUP (MIDAG)

• At Comp Sci, Psychiatry, Rad Onc, Radiology, Surgery

• Analysis Capabilities• Image Analysis• Image Display• Augmented Reality (Computer Science)• Segmentation• Registration

http://midag.cs.unc.edu

NC STATE UNIVERSITY

• BOTANY DEPARTMENT – Light Microscopy, Confocal, Nina Allen, Box 7612, NCSU

• John MacKenzie – Coordinator, EM Center john_mackenzie@ncsu.edu

• Phillip Russell – Dir, Analytical Instru. Facility Atomic Force & TEM, prussell@ncsu.edu

• Michael Dykstra – Dir, Laboratory Advanced Microscopy, TEM & Confocal michael_dykstra@ncsu.edu

• VET SCHOOL – X-ray, CT, ultrasound, MRIIan Robertson, ian_robertson@ncsu.edu

DUKE-UNC BRAIN IMAGING & ANALYSIS CENTER (BIAC)

• fMRI Neuroscience acquisition and analyses

http://www.biac.duke.edu/

POSSIBLE APPLICATIONS(from NIH proposal – T. Van Dyke & B. Tsui)

High-resolution pinhole SPECT studies:

• Can do a series of images with a single injection depending upon the length of the half-life of the radionucleotide.

• Locating tumors and bone metastases

microPET studies:

• Locating a large variety of primary tumors and their metastases

POSSIBLE APPLICATIONS (from NIH proposal – T. Van Dyke & B. Tsui)

MRI and MRS studies:• Tumor growth • Changes in tumor vascularityOptical Imaging studies:• Luciferase localization• Reporter gene expressionMicroCT studies:• Tumor growth

FUTURE DEVELOPMENTS(from NIH proposal – T. Van Dyke & B. Tsui)

• Use Animal Model systems for refining specific imaging technologies—i.e. technologies to measure blood flow and microvascular permeability within tumors.

• Imaging results compared to histologic analyses can help develop technologies to measure disease progression and then be used to screen development of disease in backgrounds with additional genetic alterations.