Lecture #20-Intro to Metabolism-29 (2019) copyprofkatz.com/courses/wp-content/uploads/2019/06/Lecture-20-Intro-to-Metabolism-29-2019...Catabolism vs Anabolism Anabolism Synthesis and

CH2210 Introduction to Metabolism Cell Structure and Function

Introduction to Metabolism Cell Structure and Function

Cells can be divided into two primary types

prokaryotes - Almost all prokaryotes are bacteria

eukaryotes - Eukaryotes include all cells of multicellular organisms, and many single-celled organisms such as the yeasts and the protists.

Introduction to Metabolism - Cell Structure and Function

Anatomy of a Typical Eukaryotic Cell

The d i f f e re nce s be t w e e n p r o k a r y o t e s a n d eukaryotes l ie in their internal organization and modes of reproduction.

In eukaryotic cells, many of the cellular macromolecules a r e p a c k a g e d i n t o o r g a n e l l e s , s u b c e l l u l a r structures surrounded by their o w n m e m b r a n e s . P r o k a r y o t e s l a c k t h i s internal organization.

Introduction to Metabolism - Cell Structure and Function

General Functions of Metabolism

Obtain energy in a chemical form by degradation of nutrients

Convert a wide variety of nutrient molecules into the central precursor molecules needed to build proteins,

carbohydrates, nucleic acids, and lipids

Synthesize cell molecules.

Modify and repair the biomolecules necessary for specific functions in specialized cells or both.

1

2

3

4

Catabolism vs Anabolism

Catabolism Biochemical Degradation of Energy-Containing Compounds

1 Capture of the Energy in New Chemical Forms

(ATP, GTP, NADH, NADPH, FADH2) 2

Conversion of Energy-Containing Compounds into a small number of simple molecules

(Pyruvate, Acetate, Krebs Cycle Intermediates)

Catabolism vs Anabolism

Anabolism

Synthesis and Repair of Biomolecules 1

Glucose → Other Monosaccharides and Polysaccharides 2

Acetate → Fatty Acids 3

Glycerol + Fatty Acids → Triglycerides 4

Lipids + Polar Components → Cell Membranes 5

Amino acids → Proteins, Hormones, Neurotransmitters 6

Nucleotides → ATP, Coenzymes, Nucleic Acids

Transport of Substances Across Membranes

Stages of Catabolism for Energy Production

Nutrient molecules are degraded to lower-molecular mass components.

The products of stage 1 are converted into one simple molecule: Accetyl-S-Coenzyme A (Acetyl-S-CoA).

Acetyl-S-coenzyme A is oxidized to CO2 and H2O by the Citric Acid Cycle.

Reduced coenzymes are oxidized through the Electron Transport Chain to yield ATP.

1

2

3

4

Stages of Catabolism

Amino AcidsGlucoseand

Other Sugars

Fatty acids and Glycerol

Stages of Catabolism FOOD

Fats Carbohydrates ProteinsStage 1

Bulk food is digested in the stomach and

small intestine to yield small molecules.

Digestion

Stages of Catabolism

Stage 2

Small sugar, fatty acid, and amino acid

molecules are degraded in cells to yield

Acetyl-CoA.

Glycolysis β-Oxidation Pathway

Amino AcidCatabolism

Amino Acids

Glucoseand

Other SugarsFatty acids

and Glycerol

Acetyl CoA Production

CH3 C

O

CoA

1

2

3

4

Stages of Catabolism

Nutrients New Forms of Energy

During the second and third stages of catabolism, important “high energy” compounds are formed that fuel anabolism:

Adenosine triphosphate (ATP) Carrier of energy.

Nicotinamide adenine dinucleotide (NADH) Carrier of reducing power

Nicotinamide adenine dinucleotide phosphate (NADPH) Carrier of reducing power

Flavin adenine dinucleotide (FAD) Carrier of reducing power

Flavin adenine mononucleotide (FMN) Carrier of reducing power

Synthesis of ATP

Adenosine-O--P--P Adenosine-O--P--P--P

R--PNutrients

Substrate level phosphorylation: Direct transfer of a phosphate group from a high energy phosphate

compound to adenosine diphosphate

Synthesis of ATP

Adenosine-O--P--P Adenosine-O--P--P--P

PEnergy

High energy electrons

Oxidative phosphorylation: Direct addition of inorganic phosphate to ADP during the simultaneous oxidation of reduced

cofactors by the electron transport chain

ATP

N

NN

N

NH2

O

OHOH

HHH

CH2

H

OP

O

O

O-

P

O

O-

O-

P

O

-O

O-

Energy is used to Fuel Anabolic Processes:

Biosynthesis Active transport

Muscle contraction Transcription, translation

N

NN

N

NH2

O

OHOH

HHH

CH2

H

OP

O

O

O-

P

O

O-

O-

P

O

-O

O- ATP

N

NN

N

NH2

O

OHOH

HHH

CH2

H

OP

O

O

O-

P

O

-O

O-

ATP

ADP

AMP

PO43-

PO43-

Energy

Energy

H2O

H2O

ADP

N

NN

N

NH2

O

OHOH

HHH

CH2

H

OP

O

-O

O-

ATP

ADP

AMP

PO43-

PO43-

Energy

Energy

H2O

H2O

AMP

Utilization of ATP

N

N

N

NH2

N

O

OH

HHH

CH2

HOH

PO

O

O

O

PO

O

O

N

O

OH

H HH

H2C

HOH

C

O

NH2

H

NAD+

+ N

C

O

NH2

H H

NADH,H+

Reduction and Oxidation of Coenzymes

N

N

N

NH2

N

O

OH

HHH

CH2

HOH

PO

O

O

O

PO

O

O

CH2CCCCH2

OHOHOH

HHH

N N

N

NO

OH

N N

N

NO

OH

H

H

FADH2FAD

Forms of Hydrogen in Oxidation/Reduction Reactions

H H

H H

H H+-

hydrogen molecule

hydrogen atoms

hydrogen ionhydride ion

The carriers of energy in oxidation-reduction reactions are actually the electrons, but the

hydrogens tell us where the electrons are.

Reduction and Oxidation of Coenzymes

Certain cofactors link metabolic oxidations to metabolic reductions. These oxidations and reductions can be visualized as dehydrogenations and hydrogenations, the removal and addition of hydrogen ions and electrons.

Coenzyme(oxidized)

Coenzyme-H2(oxidized)

BH2

BA

AH2

( reduced )

Metabolic intermediates

Metabolic intermediates

Coenzyme(oxidized)

Coenzyme-H2(oxidized)

BH2

BA

AH2

Coenzyme As an oxidizing agent As a reducing agent

Nicotinamide adenine dinucleotide NAD+ NADH/H+

Nicotinamide adenine dinucleotide phosphate NADP+ NADPH/H+

Flavin adenine dinucleotide FAD FADH2

Flavin adenine mononucleotide FMN FMNH2

Reduction and Oxidation of Coenzymes

( reduced )

Metabolic intermediates

Metabolic intermediates

+ H+N

C

O

NH2

H H

NADH,H+

Reduction and Oxidation of Nicotinamide Cofactors

( H-, H+ )

N

N

N

NH2

N

O

OH

HHH

CH2

HOH

PO

O

O

O

PO

O

O

N

O

OH

H HH

H2C

HOH

C

O

NH2

H

NAD+

This reduced form is later reoxidized by passing its electrons to molecular o x y g e n t h r o u g h t h e electron transport chain in the mitochondria.

N

N

N

NH2

N

O

OH

HHH

CH2

HOH

PO

O

O

O

PO

O

O

CH2CCCCH2

OHOHOH

HHH

N N

N

NO

OH

Reduction and Oxidation of Flavin Cofactors

H H,FAD

C CH CH C

H H O

O

O

O

C CH CH C

O

O

O

O

FAD FADH2

Succinate

Fumaratesuccinate

dehydrogenase

This reduced form is later reoxidized by passing its electrons to molecular

oxygen through the electron transport chain in the mitochondria

N N

N

NO

OH

H

H

FADH2

Stages of Catabolism

Digestion

Acetyl CoA Production

Stages of Catabolism

Citric Acid Cycle

ATP Production

2 H+ + ½ O2 H2O

NADH,H+ FADH2

electrons electrons

ATP synthase

Electron Transport System

ADP + Pi ATP

pyruvate

Acetyl CoA

glycerolderivatives

fats carbohydrates proteins

DIETARY CARBON

fatty acids monosaccharides amino acids

TCACYCLE

CO2

CO2

NADH/H+

FADH2

ATPATP

1

2

3 4

“N”