Laboratory of Applied Stress Microbiology · 2017. 6. 4. · 29 "2017 # Laboratory of Applied Stress Microbiology Manhattan from Hoboken Originality !! Serendipity !! Manhattan from

29 2017

Laboratory of Applied Stress Microbiology

Manhattan from Hoboken

Serendipity !! Originality !!

Manhattan from Empire State Building

1982 1995 2006

Applied Molecular MicrobiologyCiti Field, New York

2 1

DC 5 MC 8 + 6 8 !!

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!!

吟醸香 AAT

(ATF1/2)�Isoamyl acetate�

TA�

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Keywords etc.

Keywords ROSNO

Rim15 TOR etc

Rim15

British Columbia etc.

pH etc.

!

•  • • • 

mRNA!

!

!

X!

response

tolerance

(adaptation)

L-

N-

N- -GSA

L-

L-

L- -γ- (GSA)

L-

L-

L- -5-

L-

N- -L-

Arg2

Arg8

Arg6

Arg5

Arg7

P5C/GSA

L-

Pro2

Pro3

L-Δ1- -5- (P5C) H2O

Arg3,1,4 Car1

Pro1 Pro1

N- -5-

Mpr1 Put1

Put2

CoA

Car2

NO Tah18

ROS ?

AZC

γ-

TOR

Appl. Microbiol. Biotechnol., 47, 405, 1997; 79, 273, 2008, 81, 211, 2008; FEMS Microbiol. Lett., 184, 103, 2000; Appl. Environ. Microbiol., 69, 212, 2003; 69, 6527, 2003; 71, 8656, 2005; 73, 4011, 2007; 74, 5845, 2008; J. Biosci. Bioeng., 94, 2002; 100, 538, 2005; 103, 277, 2007; 116, 576, 2013; Biosci. Biotech. Biochem., 73, 2131, 2009; 76, 454, 2012; Int. J. Food Microbiol., 152, 40, 2012; 238, 233, 2016; J. Gen. Appl. Microbiol., 62, 132, 2016; Yeast, 33, 353, 2016; FEBS Lett., 590, 2906, 2016; Microbial Cell, 3, 522, 2016.

Arg

Mpr1

Mpr1

S. cerevisiae Σ1278b：AZC 耐性株�

AZC N-

ROS

Pro/Glu Arg NO

J. Bacteriol., 182, 4249, 2000; J. Biol. Chem., 276, 41998, 2001; Yeast, 19, 1437, 2002; 26, 587, 2009; J. Biochem., 133, 67, 2003; 138, 391, 2005; Proc. Natl. Acad. Sci. USA, 101, 12616, 2004; 110, 11821, 2013; Appl. Microbiol. Biotechnol., 75, 1343, 2007; 97, 247, 2013; FEMS Yeast Res., 8, 607, 2008; Biotechnol. Bioeng., 103, 341, 2009; Int. J. Food Microbiol., 138, 181, 2010; J. Biosci. Bioeng., 114, 160, 2012; J. Biochem., 159, 271, 2016 etc.

CO2HNH

L-����-�� �� (AZC)

NH

CO2H

HO

��-4-����-L-���� (CHOP)

NO

!!

mpr1Arg NO

NO Tah18-Dre2 NO

NO Mac1

NO etc.

NO Mpr1 Arg Tah18 NO

NO Mac1

Mac1�

Nucleus�

NO�

CTR1�

Arg�

Ctr1�Cu+�

Ctr1�

Fre1�

Cu2+�

ROS�

Tah18� Sod1�

Sod1�Sod1�S-����� ?�

ストレス耐性 !! 高温�

FEMS Yeast Res., 10, 687, 2010; Microb. Cell Fact., 11:40 doi:10.1186/1475-2859-11-40, 2012: Proc. Natl. Acad. Sci. USA, 110, 11821, 2013; Biochem. Biophys. Res. Commun., 430, 137, 2013 PLoS One, 9, e113788, 2014; Nitric Oxide-Biol. Chem., 52, 29, 2016; 57, 85, 2016; Appl. Microbiol. Biotechnol., 100, 9483, 2016.

Rsp5

Rsp5

Rsp5

Rsp5

Rsp5

Rsp5 AZC

Proc. Natl. Acad. Sci. USA, 100, 11505, 2003; FEBS Lett., 580, 3433, 2006; Biosci. Biotech. Biochem., 70, 2762, 2006; 73, 2268, 2009; FEMS Microbiol. Lett., 277, 70, 2007; Genes Cells, 13, 105, 2008; FEMS Yeast Res., 9, 73, 2009; 14, 567, 2014; FEBS J., 276, 5287, 2009 ; J. Brew. Distill., 3, 1, 2012, Genes Cells, 18, 459, 2013; Eukaryot. Cell, 13, 1191, 2014; J. Biochem., 157, 251, 2015 etc.

rim155055insA

Rim15 !!

Rim15

Greatwall

Rim15Appl. Environ. Microbiol., 77, 934, 2011; 78, 385, 2012; 78, 4008, 2012; J. Biosci. Bioeng., 112, 44, 2011; 112, 54, 2011; 112, 577, 2011; 116, 591, 2013; 123, 183, 2017; Biosci. Biotech. Biochem., 77, 2255, 2013; Appl. Environ. Microbiol., 82, 340, 2016.

!!

FEMS Yeast Res., 16, fow049, 2016; J. Cell Sci. 128, 1762, 2015; Biosci. Biotechnol. Biochem., 78, 1389, 2014; Cell Struct. Funct., 38, 135, 2013; Biosci. Biotechnol. Biochem., 77, 1337, 2013; Genes Cells 18, 288, 2013; Mol. Biol. Cell, 22, 3520, 2011; Curr. Opin. Cell Biol., 23, 135, 2011; J. Cell Biol., 179, 75, 2007; J. Cell Biol., 167, 445, 2004; Mol. Biol. Cell, 14, 2559, 2003.

ER !!

ER ?

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��"%)"#* +Ire1

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"%)"�����

��"%)"�

小胞体ストレスに伴う、Ire1の集合

ストレス無し������������������� ストレス時�(DTT$10mM$30min) �

Ire1-GFPの細胞内分布

Ire1

小胞体 サイトゾル�

�����

ER

etc.

Ire1 ER

? !!

ER

NONO

! Rsp5

→ Rsp5 α-

Univ. Medical Center Goettingen Univ. Bayreuth (Geremany)

!!

!! !!

!!

The 10th ANNIVERSARY

!!

, , , , , , , , , , , , , , , J&J,

, , , , , ,

!!

!!

5 International Meeting on Yeast Apoptosis (Italy), NO 6 International Specialized Symposium on Yeast (Ireland) 7 International Union of Microbiological Societies (Singapore)

9 , 12 , 3 etc.

!!

!!

5 M1 Beer & BBQ

12 Bowling → Eating/Drinking → Singing

3

!!