INVESTIGATING S100A4 AS THERAPEUTIC TARGET VIS-À-VIS EMPLOYABILITY OF AYURVEDIC CONCEPTS FOR ADVANCED RESEARCH AND CLINICAL RELEVANCE Vivek Sharma Scientist.

INVESTIGATING S100A4 AS THERAPEUTIC TARGET VIS-À-VIS

EMPLOYABILITY OF AYURVEDIC CONCEPTS FOR ADVANCED RESEARCH AND CLINICAL RELEVANCE

Vivek SharmaScientist In-chargeDivision of Cancer ResearchDepartment of BiotechnologyChandigarh Group of Colleges, Mohali &

Founder, Anvaya Biosciences (a start-up company)

Ph. No. +91-8054 66 7211; E-mail: biotechno16@gmail.com

S100A4 AT A GLANCE!

FOCUS OF RESEARCH

IMPORTANT RESULTS

S100A4 under normal conditions does not

affect the cell polarity.

S100A4 triggers JNK pathway in metastatic

conditions.

Analyzing S100A4 as a therapeutic target.

INTERACTION BETWEEN FENUGREEKINE AND 3C1V

FENUGREEKINE

Fig. (1a) Distances between active site residues of 3C1V and Fenugreekine in Angstrom (Å)

Fig. (1b) Fitting of Fenugreekine in the active site cavity of 3C1V.

INTERACTION BETWEEN GERANIOL AND 3C1V

GERANIOL

Fig. 2: Fitting of Geraniol in the active site cavity of 3C1V.

SIMULATION STUDIES

Fig. 3: Simulation studies and comparison of potential energies and temperature parameters between (a) 3C1V, (b) 3C1V-Fenugreekine and (c) 3C1V-Geraniol complex.

a

ba

b c

c

QUESTION IN FOCUS!

In the current day clinical practice, what treatment strategy is adopted to treat a patient with metastasis?

Best answer one can get is combinatorial therapies. Looking at new targetsNew mutationsFinding out new molecules

THE TRANSLATIONAL SIGNIFICANCE!

Yes they are.

But, how much comprehensively these therapies are

applicable?

What is the success rate?

Are these therapies treating the disease or just managing it?

Are the fields of genomics, proteomics and metabolomics really

helping us in finding out the therapies for diseases?

Are the processes of pre-clinical trials, clinical trials (Phase I, II,

III) and the pharmacovigilance robust enough?

SYSTEMS APPROACH

MAJOR ELEMENTS AND REQUIREMENTS OF SYSTEMS BIOLOGYMolina F et al. (2010)

AYURVEDA

Ayurveda is Upveda of ‘Athraveda’ and its practice can

be traced back to 3000 BCE.

Is the oldest system of medicine known in the world

with reported evidences by the sages and saints of

ancient India.

It deals with the holistic understanding of the disease,

followed by holistic treatment.

BASIC PRINCIPLES OF AYURVEDA

Principle of Tridosha – Vata, Pitta, Kapha Principle of Panchmahabhutas – Akash (Space), Vayu (Air),

Jal (Water), Agni (Fire), Prithvi (Earth) Principle of Saptdhatu – Rasa (Plasma), Rakta (blood cells),

Mamsa (muscle tissue), Meda (fatty tissue), Asthi (bone tissue), Majja (bone marrow and nervous tissue), Shukra (reproductive tissues)

Principle of Srotvijanana – which is about Macrocosm – microcosm continuum and it correlates with Quantum Biology concept.

Discipline of Bhaisjya Kalpana – field of Drug delivery in Ayurveda

INTEGRATING THE KNOWLEDGE!

Can we integrate the knowledge of Ayurveda with the tools of genomics and proteomics to build a platform which can be used for development of truly integrative and personalized medicine?

Can we bring in the original principles of Ayurveda in modern day research models?

REPORTS

HOW CAN WE DO IT?

INTEGRATING THE SCIENCE OF AYURVEDA WITH MODERN TECHNOLOGIES

www.anvayabio.comE-mail - anvayabios@gmail.com

OUR FOCUS

To create a amalgamated platform for bringing together the molecular diagnostics, Ayurveda diagnostics and treatment strategies from both fields with personalized approach.

To create a library of plant based compounds and there derivatives having potential therapeutic effect in cancer (for example).

To create awareness among Ayurveda fraternity for importance of molecular tools and techniques and how these tools can be helpful in validating the ancient knowledge for its global acceptance.

ACKNOWLEDGEMENTS Prof. (Dr.) Upendra K. Jain, Director – Principal,

Chandigarh College of Pharmacy, CGC, Mohali. Mrs. Palki Sahib Kaur, HOD – Dept. of Biotechnology,

CGC, Mohali. Collaborators:

Mr. Rakesh Kr. Pandit, Assistant Professor, Bioinformatics, Dept. of Biotechnology, CGC.

Mrs. Piyusha Sharma, Anvaya Biosciences Mr. Aniket Sinha, Anvaya Biosciences

Students:• Navpreet Kaur• Shikha• Pooja Thakur• Manpreet Kaur

THANK YOUQUESTIONS ARE

INVITED!