Image-Pro Premier Basic Training Course

Image-Pro PremierBasic Training Course

Part 7 - Image Enhancements

Image Enhancements

Enhancements can be made for several reasons:AestheticsFunctionalityPracticality

If the image is modified, the process must be:SystematicJustifiable

Always make a copy of the image before

processing!

Display Enhancement

Use Look-Up Tables to adjust how pixels are displayed on the screenNon-destructive unless appliedDoesn’t generally improve ability to analyse images

Filters

Process individual pixels based on their environmentUsed to:

enhance or reduce noisePull out EdgesEnhance morphological feature

Masks

Create binary images for processingCan be used to extract further information from objectsUse from Count/Size to fill holes or add smoothing

Colour Extraction

Colour increases the complexity of analysis

Colour isn’t always necessary or useful

Colour images can be converted to mono from the Adjust ribbon by either:

Extracting colour channels

Averaging information from all channels

Colour Extraction

Mono Average

Colour Separation

Background Correction

Uneven background can be aesthetically jarring and render analysis difficult

Use background Correction to flatten backgrounds by:

Subtracting background image

Estimating background

Colour Composites

Fluorescence imaging is typically monochromeMultiple images are taken separately from the same sampleColour composites recombine multiple monochrome images into a single workspace