IGF-1 GENE EXPRESSION: an instantaneous growth indicator for tilapia Emmanuel Vera Cruz, Christopher Brown, Remedios Bolivar, Russell Borski, students,

IGF-1 GENE IGF-1 GENE EXPRESSION: an EXPRESSION: an

instantaneous growth instantaneous growth indicator for tilapiaindicator for tilapia

Emmanuel Vera Cruz, Emmanuel Vera Cruz, Christopher Brown, Christopher Brown, Remedios Bolivar, Russell Remedios Bolivar, Russell Borski, students, and Borski, students, and colleaguescolleagues

Cloning, diet (2006) Cloning, diet (2006) Aquaculture 251:585-595. Aquaculture 251:585-595.

Social Status (2007) Hormones Social Status (2007) Hormones and Behavior. 51:611-619.and Behavior. 51:611-619.

Temperature dependence, in Temperature dependence, in presspress

Influence of photoperiod, in press Influence of photoperiod, in press

Isolation of IGF-1 Isolation of IGF-1 mRNA, mRNA, Oreochromis Oreochromis

niloticusniloticus 1 TTCTCCAAAACGAGCCTGCGCAATGGAACAAAGTCGGAATATTGAGATGTGACATCGCC 59 60 CGCATCTCATCCTCTTTCTCCCTGTTTTAATGACTTTAAACAAGTTCATTTTCGTCGGGC 119 Signal Peptide (144-275) 120 TTTGTCTTGTGGAGACCCGTGGGGATGTCTAGCGCTTTTTCCCTTCAGTGGCATTTATGT 179 M S S A F S L Q W H L C 180 GATGTCTTCAAGAGTGCGATGTGCTGTATCTCCTGTAGCCACACCCTCTCACTACTGCCG 239 D V F K S A M C C I S C S H T L S L L P B domain(276-362) 240 TGCGTCCTCACCCTGACTCCGACGGCAACAGGGGCGGGCCCTGAGACCCTGTGCGGGGCG

299 C V L T L T P T A T G A G P E T L C G A 300 GAGCTGGTCGACACGCTGCAGTTTGTCTGTGGAGAGCGAGGCTTTTATTTCAATAAACCA 359 E L V D T L Q F V C G E R G F Y F N K P C domain (363-392) A domain (393-455) 360 ACAGGCTATGGCCCCAGTGCAAGGCGGTCACGTGGCATCGTGGACGAGTGCTGCTTCCAA 419 T G Y G P S A R R S R G I V D E C C F Q D domain (456-479) 420 AGCTGTGAGCTGCAGCGCCTTGAGATGTACTGTGCACCTGTCAAGACTCCCAAGATTTCT 479 S C E L Q R L E M Y C A P V K T P K I S E domain (480-539) 480 CGCTCTGTGCGTTCACAGCGGCACACAGACATGCCAAGAGCACCCAAGGTTAGTAGCAGA 539

Close (84-90%) sequence identity with other IGF-1s

Hepatic gene Hepatic gene expression as related expression as related

to weight gainto weight gain

Temperature-Temperature-dependent differences dependent differences in weight gain and IGF-in weight gain and IGF-

1 expression1 expression

Effect of environmental Effect of environmental temperature on IGF-1 temperature on IGF-1

expressionexpression

Social dominance Social dominance and IGF-1 expressionand IGF-1 expression

Correlation of Correlation of aggression w/ IGF-1 aggression w/ IGF-1

expressionexpression

Photoperiod-induced Photoperiod-induced changes, growth & IGF-changes, growth & IGF-

11

Temperature Temperature influence on growth influence on growth

raterate

Correlative change Correlative change in IGF-1 expression…in IGF-1 expression…

… … resulting from tight resulting from tight association of growth association of growth with IGF-1 expressionwith IGF-1 expression

AcknowledgementsAcknowledgements Funding agenciesFunding agencies

USAID/PDA CRSPUSAID/PDA CRSP The Fulbright FoundationThe Fulbright Foundation

InstitutionsInstitutions Central Luzon State Central Luzon State

UniversityUniversity North Carolina State North Carolina State

UniversityUniversity Florida International Florida International

UniversityUniversity NOAA, The Milford NOAA, The Milford

LaboratoryLaboratory

Thank you!Thank you!