GUSTA ME - univie.ac.atcolisci.univie.ac.at/uploads/media/pres_colisci_gustame.pdf · GUSTA ME Motivation GUSTA ME End-points Wizards Walkthroughs VIZ libraries MASAME Community Usage

GUSTA ME

¿Te gusta la estadística?

An online guide to statistical analysis in microbial ecology

Roman FeldbauerDivision of Computational Systems Biology

GUSTA ME

Motivation

GUSTA ME

End-points

Wizards

Walkthroughs

VIZ libraries

MASAME

Community

Usage ex.

Outlook

● Multivariate statistical analysis in microbial ecology

● Which technique for which data?● Ongoing development of methodology● 'Prevalence rate' of statistical

expertise?

→ Where to find guidance?

GUSTA ME

Motivation

GUSTA ME

End-points

Wizards

Walkthroughs

VIZ libraries

MASAME

Community

Usage ex.

Outlook

● Multivariate statistics for microbial ecologists

● Online resource● Techniques, relevance, debates● Different entry points/approaches

● GUSTA ME webpage:

http://mb3is.megx.net/gustame

GUSTA ME

Motivation

GUSTA ME

End-points

Wizards

Walkthroughs

VIZ libraries

MASAME

Community

Usage ex.

Outlook

● Reference pages● High-level descriptions of techniques● Principles, assumptions, limitations,

statistical and ecological interpretation plus common pitfalls

● Examples: PCA, NMDS, db-RDA, ...

GUSTA ME

Motivation

GUSTA ME

End-points

Wizards

Walkthroughs

VIZ libraries

MASAME

Community

Usage ex.

Outlook

Interactive guides:● Suitable methods for given data set● Decrease and conquer strategy● 'Decision tree' based on (relatively)

easy questions● Suggestion of one or several

appropriate techniques

GUSTA ME

Motivation

GUSTA ME

End-points

Wizards

Walkthroughs

VIZ libraries

MASAME

Community

Usage ex.

Outlook

● Social cognitive theoretic approach● Flowcharts: Analytical methodology

of peer-reviewed studies● Learning by imitation

● http://mb3is.megx.net/gustame/ walkthroughs

GUSTA ME

Motivation

GUSTA ME

End-points

Wizards

Walkthroughs

VIZ libraries

MASAME

Community

Usage ex.

Outlook

● Visualization makes high-dimensional data human readable

● Browsing different types of viz● 'Putting the cart before the horse'

approach

● http://mb3is.megx.net/gustame/home/ visualisations

GUSTA ME

Motivation

GUSTA ME

End-points

Wizards

Walkthroughs

VIZ libraries

MASAME

Community

Usage ex.

Outlook

● Multivariate AnalysiS Applications in Microbial Ecology

● Web front-end for several R tools● Point-and-click style, no R knowledge

required● Example data sets provided● Direct links from end-points

(e.g. PCA, NMDS, db-RDA)

GUSTA ME

Motivation

GUSTA ME

End-points

Wizards

Walkthroughs

VIZ libraries

MASAME

Community

Usage ex.

Outlook

● Community involvement● Online forums

– Comments on content

– Method discussion

– Suggest revisions

– Contribute walkthroughs etc.

● Community still needs to grow...

GUSTA ME

Motivation

GUSTA ME

End-points

Wizards

Walkthroughs

VIZ libraries

MASAME

Community

Usage ex.

Outlook

● Student– Given data set, follow a wizard, analysis using

MASAME, publish study with appropriate multivariate statistics

● Principal investigator– Designing a study, browses walkthroughs, adjusts

sampling design and replication strategy

● Reviewer– Needs to check appropriateness of a certain

technique, jumps directly to corresponding end-point, may check key assumptions with MASAME

GUSTA ME

Motivation

GUSTA ME

End-points

Wizards

Walkthroughs

VIZ libraries

MASAME

Community

Usage ex.

Outlook

● Current state is a starting point, useful resource for multivariate statistics

● Might become great knowledge base● Some issues

– Community involvement

– Minor inconsistencies

– Web design

GUSTA ME

Motivation

GUSTA ME

End-points

Wizards

Walkthroughs

VIZ libraries

MASAME

Community

Usage ex.

Outlook

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