Glucosyltransferases Alternan Mutan Dextran Glucosyltransferases Leuconostoc mesenteroides Streptococcus linkages 1-6 B-512F B-1299 B-1355 1-3 1-2.
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Glucosyltransferases
O
O
O
O
OHO
O
Polymer synthesis
OHO
HOHO
HO
HO
HOOH
OHOO
+
OHO
OHO
Hydrolysis
Acceptor reactions
HOHO
HOHO
HOHO
HO
HOHO
HO
HO
HO
HO
HO
HO
HOHO
HOHO
HOHO
HO
HO
HOHO
HO
HO
HO
HO
HO
HO
HOHO
HOHO
HOHO
HOHO
HO
HO
HO
O
O
O
O
OH
OH
OH
OHO
OO
O
O
HOMaltose
Sucrose
Dextran
Glucose
+
+
Panose
Fructose
Fructose
Fructose
n
Alternan
Mutan
Dextran
Glucosyltransferases
Leuconostoc mesenteroides
Streptococcus
linkages
1-6B-512F
B-1299
B-1355
1-3
1-2
mutans GS5
downei Mfe28
Microorganism enzyme
Dextransucrase
Alternansucrase
Mutansucrase
Glucansucrase
1-6
1-6 1-3
1-6 1-3
1-6 1-3
strain polymer
Glucan
O
O
OO
O
O
O
CH2OH
CH2OHCH2
OHOH
OH
OH
OH
OH
OH
OHOH
HOCH2
Oligosacaridos
-Fructo-oligosacáridos
-Fructo-oligosacáridos
-Galacto-oligosacáridos
-Galacto-oligosacáridos
Lactulosa
-Gluco-oligosacáridos
-Gluco-oligosacáridos
-Gluco-oligosacáridos
Xylo-oligosacáridos
Proceso
Síntesis Enzimática
Hidrólisis Enzimática
(inulina)
Extracción de Soya
Síntesis Enzim{atica
Isomerización de lactosa
Síntesis Enzimática
Hidrólisis de Almidón
Síntesis Enzimática
Hidrólisis Enzimática
(Hemicelulosa)
Empresa
Meiji Seika
Refinería
Tirlemontoise
Calpis
Yakult
Nikken Kapau
Meiji Seika
Showa Sangyo
BioEurope
fuente: Monsan P. Biofutur, (6,7), 1993
Bioprospection
Agave PozolPulque
Pozol
14 strains of Leuconostoc mesenteroides
Cell-associated activity
Identify as Leuconostoc citreum by analysis of 16S
rRNA
screening of glucosyltransferases activities
FRUCTOSYLTRANSFERASE
o
Fructose
ooHO
HO
OHHO
HO
OHHO
OH
o
HO
HOHO
HO o
Sucrose
Polymer synthesis
(n)
O O OO
o
HO
HO
HOCH2
O O
HO
HO CH2OHCH2OH
CH2 CH2
CH2OHHO
HO
O CH2
CH2OHHO
HO
O
CH2
HO
HO
HOHO
HO
HOHO
HO o
OH
+
Levan
Glucose
Acceptor reactions
Fructosyltransferases
HO
HOHO
HO o
OH
Glucose
+
Hydrolisis
+HO
HOHO
HO o
OH
Glucose
HOHO
HOHO
HO
HO
HO
O
OO
HO
Maltose
HOHO
HOHO
HO
HO
HO
O
OO
OO
HO
HO
OHHO
Fructoside
ß-2,1 linked fructosyl moieties with branching through ß-2,6 linkages
(n)
O
o
HO
HO
O
CH2
HO
HO
HOHO
CH2
CH2OH
O
HO
HO
O
CH2
CH2OH
O
HO
HO
OCH2OH
CH2OH
Inulin
Levan
ß-2,6 linked fructosyl moieties with branching through ß-2,1 linkages
O O OO
o
HO
HO
HOCH2
O O
HO
HOCH2OHCH
2OH
CH2
CH2
CH2OHHO
HO
O CH2
CH2OHHO
HO
O
CH2
HO
HO
HOHO
(n)
Applications of fructans in food
• Technological properties
– Taste improvement
– Texturizing agent
(mouthfeel)
– Sugar and fat
replacement
– Filler/binder in tablets
• Nutritional properties
– Soluble dietary fiber
– Prebiotic and related
health effects
– Sugar and fat replacement
– Low caloric value
– Suitable for diabetics
Fructosiltransferasas
• Plantas
Neoseries de levana
LEVANALEVANA
levana
Neoseries de inulina
INULINAINULINA
levana
NEOKESTOSA
1- KESTOSA
BIFURCOSA
SACAROSA6-KESTOSA
6-SFT
6-SFT
6-SFT 6-SFT
1-FFT
1-FFT
1-FFT
6-SFT
6G-FFT
1-SST
+ Sac
+ Sac
Modelo de la biosíntesis de fructanas en plantas (Vijn y Smeekens, 1999).
©SCIMAT 2003
• Bacterias
SACAROSA
INULINAINULINA
LEVANALEVANA
IS
LS
Azucar Caña de azúcar
Enzima
Fructosiltransferasa
Glucosa
Fructosa
Fructosa
Fructosa
Fructosa
FOS
Glucosa
+
Fructosa
Sacarosa =
1 tea spoon of NutraFlora
22 bananas15 onions383 cloves of garlic
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