Transcript
DNA
DNAIt’s gonna getchoo.
The basics
Space-filling Model
Super-simplified
DNA in the cell
Transcription
Transcription
Translation
Or you can watch it . . .
Junk DNA?
Junk DNA?
Tandem Repeats
A-T-T-C-G-A-T-T-C-G-A-T-T-C-G
Tandem Repeats
RFLP
RFLP
RFLP
Gel Electrophoresis
PP doesn’t like flash . . .
Gel Electrophoresis Animation
A run gel.
DNA RFLP Comparison
BUT . . .
BUT . . .
•Downsides of RFLP:
BUT . . .
•Downsides of RFLP:•Large DNA fragments
needed
BUT . . .
•Downsides of RFLP:•Large DNA fragments
needed•Large original samples
needed
BUT . . .
•Downsides of RFLP:•Large DNA fragments
needed•Large original samples
needed•Fresh samples a must
BUT . . .
•Downsides of RFLP:•Large DNA fragments needed•Large original samples
needed•Fresh samples a must•Testing is lengthy (3wks – 3
months)
Short Tandem Repeats
Short Tandem Repeats
We are a type of tandem repeat . . .
Short Tandem Repeats
We are a type of tandem repeat . . .
BUT TINY!
The 13 CODIS STR’s and
their Identity ProbabilitiesSTR African American U.S. Caucasian
D3S1358 0.094 0.075
vWA 0.063 0.062
FGA 0.033 0.036
TH01 0.109 0.081
TPOX 0.090 0.195
CSF1PO 0.081 0.112
D5S818 0.112 0.158
D13S317 0.136 0.085
D7S820 0.080 0.065
D8S1179 0.082 0.067
D21S11 0.034 0.039
D18S51 0.029 0.028
D16S539 0.070 0.089
Fortunately!
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