Cellular respiration ppt wit turning pt qs

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Cellular Cellular RespirationRespiration

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Cellular RespirationCellular Respiration A catabolic, exergonic, oxygen (Ocatabolic, exergonic, oxygen (O22))

requiring process that uses energyenergy extracted from macromolecules (glucose)macromolecules (glucose) to produce energy (ATP)energy (ATP) and water (Hwater (H22O).O).

C6H12O6 + 6O2 6CO2 + 6H2O + energyglucoseglucose ATP

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Question:Question: In what kinds organisms does In what kinds organisms does

cellular respiration take place?cellular respiration take place?

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Plants and AnimalsPlants and Animals Plants - AutotrophsPlants - Autotrophs: self-producers. Animals - Heterotrophs: consumers.

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MitochondriaMitochondria OrganelleOrganelle where cellular respirationcellular respiration takes

place.

Innermembrane

Outermembrane

Innermembrane space

Matrix Cristae

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Redox ReactionRedox Reaction Transfer of one or more electrons

from one reactant to another.

Two types:Two types:1.1. OxidationOxidation2.2. ReductionReduction

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Oxidation ReactionOxidation Reaction The lossloss of electrons from a

substance. Or the gaingain of oxygenoxygen.

C6H12O6 + 6O2 6CO2 + 6H2O + energy

glucose ATP

OxidationOxidation

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Reduction ReactionReduction Reaction The gaingain of electrons to a

substance. Or the lossloss of oxygenoxygen.

glucose ATP

C6H12O6 + 6O2 6CO2 + 6H2O + energy

ReductionReduction

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Breakdown of Cellular Breakdown of Cellular RespirationRespiration

Four main parts (reactions).Four main parts (reactions).

1. Glycolysis (splitting of sugar)1. Glycolysis (splitting of sugar)a. cytosol, just outside of mitochondria.

2. Grooming Phase2. Grooming Phasea. migration from cytosol to matrix.

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Breakdown of Cellular Breakdown of Cellular RespirationRespiration3. Krebs Cycle (Citric Acid Cycle)3. Krebs Cycle (Citric Acid Cycle)

a. mitochondrial matrix

4. Electron Transport Chain (ETC) and4. Electron Transport Chain (ETC) and Oxidative PhosphorylationOxidative Phosphorylation

a. Also called Chemiosmosisb. inner mitochondrial membrane.

The reactants in cellular respiration are:

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CO2 & O2

CO2 & C6H12O6

O2 & C6H12O6

ATP & H2O

25% 25%25%25%

1. CO2 & O2

2. CO2 & C6H12O6

3. O2 & C6H12O6

4. ATP & H2O

201 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20

21 22 23 24 25 26 27 28 29 30

Water and what are made in cellular respiration?

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CO 2 & ATP

CO2 & C6H12O6

O2 & C6H12O6

O2 & ATP

25% 25%25%25%

1. CO 2 & ATP2. CO2 & C6H12O6

3. O2 & C6H12O6

4. O2 & ATP

201 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20

21 22 23 24 25 26 27 28 29 30

Where does respiration occur?

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Nucleus

Chloroplasts

Mito

chondria

Cytoplasm

25% 25%25%25%

1. Nucleus2. Chloroplasts3. Mitochondria4. Cytoplasm

201 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20

21 22 23 24 25 26 27 28 29 30

What types of organisms use cellular respiration?

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All plants

All Anim

als

All Eukaryotes

All Pro

karyo

te...

25% 25%25%25%

201 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20

21 22 23 24 25 26 27 28 29 30

1. All plants2. All Animals3. All Eukaryotes4. All Prokaryotes

A molecule that loses electrons is:

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Oxidized

Reduced

Neutralize

d

Molecu

larized

25% 25%25%25%

201 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20

21 22 23 24 25 26 27 28 29 30

1. Oxidized2. Reduced3. Neutralized4. Molecularized

What is the definition of glycolysis?

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Makin

g suga

r

Makin

g ATP

Splitti

ng suga...

Splitti

ng ATP

25% 25%25%25%

1. Making sugar2. Making ATP3. Splitting sugar4. Splitting ATP

201 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20

21 22 23 24 25 26 27 28 29 30

Where does glycolysis occur?

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Mito

chondria

l ...

Chloroplast

Nucleus

Cytosol

25% 25%25%25%

1. Mitochondrial matrix

2. Chloroplast3. Nucleus4. Cytosol

201 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20

21 22 23 24 25 26 27 28 29 30

Where does the Kreb’s Cycle occur?

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Mito

chondria

l ...

Chloroplast

Nucleus

Cytosol

25% 25%25%25%

1. Mitochondrial matrix

2. Chloroplast3. Nucleus4. Cytosol

201 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20

21 22 23 24 25 26 27 28 29 30

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1. Glycolysis1. Glycolysis Occurs in the cytosol just outside of

mitochondria. Two phases (10 steps):Two phases (10 steps):

A. Energy investment phaseA. Energy investment phasea. Preparatory phase (first 5 steps).B. Energy yielding phaseB. Energy yielding phasea. Energy payoff phase (second 5 steps).

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1. Glycolysis1. GlycolysisA. Energy Investment Phase:A. Energy Investment Phase:

Glucose (6C)

Glyceraldehyde phosphate (2 - 3C) (G3P or GAP)

2 ATP - used0 ATP - produced0 NADH - produced

2ATP

2ADP + P

C-C-C-C-C-C

C-C-C C-C-C

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1. Glycolysis1. GlycolysisB. Energy Yielding PhaseB. Energy Yielding Phase

Glyceraldehyde phosphate (2 - 3C) (G3P or GAP)

Pyruvate (2 - 3C) (PYR)

0 ATP - used4 ATP - produced2 NADH - produced

4ATP

4ADP + PC-C-C C-C-C

C-C-C C-C-C

GAP GAP

(PYR) (PYR)

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1. Glycolysis1. Glycolysis Total Net YieldTotal Net Yield

2 - 3C-Pyruvate (PYR)2 - ATP (Substrate-level Phosphorylation)2 - NADH

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Substrate-Level Phosphorylation

ATP is formed when an enzyme transfers a phosphate groupphosphate group from a substrate to ADP.

Enzyme

Substrate

O-

C=OC-O-CH2

P P P Adenosine

ADP(PEP)Example:PEP to PYR

P PPATP

O-

C=OC=OCH2

Product(Pyruvate)

Adenosine

In glycolysis you need ___ATP and make ____ ATP

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2 , 2 2 , 4 4 , 2 4 , 4

25% 25%25%25%

1. 2 , 22. 2 , 43. 4 , 24. 4 , 4

201 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20

21 22 23 24 25 26 27 28 29 30 31 32 33 34

The phase of glycolysis where you use 2 ATP.

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25% 25%25%25%

1. Energy Yielding2. Energy

Investment3. Energy

Production4. Energy

Completion

201 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20

21 22 23 24 25 26 27 28 29 30 31 32 33 34

Glycolysis results in 2 ATP and ________.

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2 NADH & 2 pyr.

..

2 NADH & 4 ATP

2 NADH+ & 2 py..

.

2 NAHH+ & 4 AT...

25% 25%25%25%

201 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20

21 22 23 24 25 26 27 28 29 30 31 32 33 34

1. 2 NADH & 2 pyruvate

2. 2 NADH & 4 ATP3. 2 NADH+ & 2

pyruvate4. 2 NAHH+ & 4 ATP

The process of making ATP by adding Phosphate from a molecule to ADP

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ATP synthase

Oxidative Phos..

. ET

C

Substr

ate leve...

25% 25%25%25%

1. ATP synthase2. Oxidative

Phosphorylation3. ETC4. Substrate level

phosphorylation

201 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20

21 22 23 24 25 26 27 28 29 30 31 32 33 34

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Fermentation Fermentation Occurs in cytosol when “NO Oxygen”“NO Oxygen” is

present (called anaerobic).(called anaerobic). Remember: glycolysisglycolysis is part of

fermentationfermentation. Two Types:Two Types:

1.1. Alcohol FermentationAlcohol Fermentation2. Lactic Acid Fermentation2. Lactic Acid Fermentation

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Alcohol FermentationAlcohol Fermentation Plants and FungiPlants and Fungi beer and beer and

winewine

glucose

Glycolysis

CCCCCC

CCC

2 Pyruvic acid

2ATP2ATP2ADP+ 2

2NADH

P

2 NAD+

CC

2 Ethanol2 Ethanol2CO2CO22

releasedreleased

2NADH 2 NAD+

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Alcohol FermentationAlcohol Fermentation End Products: Alcohol fermentationEnd Products: Alcohol fermentation

2 - ATP (substrate-level phosphorylation)2 - CO2

2 - Ethanol’s

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Lactic Acid FermentationLactic Acid Fermentation Animals (pain in muscle after a Animals (pain in muscle after a

workout).workout).

2 Lactic2 Lactic acidacid

2NADH 2 NAD+

CCC

Glucose

GlycolysisCCC

2 Pyruvic acid

2ATP2ATP2ADP+ 2

2NADH

P

2 NAD+

CCCCCC

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Lactic Acid FermentationLactic Acid Fermentation End Products: Lactic acid End Products: Lactic acid

fermentationfermentation

2 - ATP (substrate-level phosphorylation)2 - Lactic Acids

What occurs after glycolysis if there is no O2 present?

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8%0%

88%

4%

1. Kreb’s Cycle2. ETC3. Fermentation4. Oxidative

phosphorylation

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20

21 22 23 24 25 26 27 28 29 30 31 32 33

What organisms undergo alcoholic fermentation?

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8%0%

92%

0%

1. Plants2. Fungi3. Both 1 & 24. Neither 1 nor 2

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20

21 22 23 24 25 26 27 28 29 30 31 32 33 34

What product of alcoholic fermentation gave it its name?

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ATP CO2

Ethan

ol

Lacti

c Acid

4%12%

77%

8%

1. ATP2. CO2

3. Ethanol4. Lactic Acid

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20

21 22 23 24 25 26 27 28 29 30 31 32 33 34

What organisms undergo lactic acid fermentation?

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Plants Fu

ngi

Animals

None of the ab...

0% 0%

100%

0%

1. Plants2. Fungi3. Animals4. None of the

above

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20

21 22 23 24 25 26 27 28 29 30 31 32 33 34

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2. Grooming Phase2. Grooming Phase Occurs when Oxygen is present (aerobic).Oxygen is present (aerobic). 2 Pyruvate (3C) molecules are transported

through the mitochondria membrane to the matrix and is converted to 2 Acetyl CoA (2C) molecules.

CytosolCCC

2 Pyruvate

2 CO2 CO22

2 Acetyl CoA2 Acetyl CoAC-CC-C

2NADH2NADH2 NAD+

Matrix

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2. Grooming Phase2. Grooming Phase End Products: End Products: grooming phasegrooming phase

2 - NADH2 - NADH2 - CO2 - CO22

2- Acetyl CoA (2C)2- Acetyl CoA (2C)

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3. Krebs Cycle (Citric Acid 3. Krebs Cycle (Citric Acid Cycle)Cycle) Location:Location: mitochondrial matrix. Acetyl CoA (2C) bonds to Oxalacetic

acid (4C - OAA) to make Citrate (6C). It takes 2 turns of the krebs cycle to

oxidize 1 glucose molecule.

MitochondrialMatrix

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3. Krebs Cycle (Citric Acid 3. Krebs Cycle (Citric Acid Cycle)Cycle)

KrebsCycle

1 Acetyl CoA (2C)

3 NAD+

3 NADH3 NADHFAD

FADHFADH22

ATPATP ADP + P

(one turn)(one turn)

OAA (4C) Citrate (6C)

2 CO2

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3. Krebs Cycle (Citric Acid 3. Krebs Cycle (Citric Acid Cycle)Cycle)

KrebsCycle

2 Acetyl CoA (2C)

6 NAD+

6 NADH6 NADH2 FAD

2 FADH2 FADH22

2 ATP2 ATP 2 ADP + P

(two turns)(two turns)

OAA (4C)Citrate (6C)

4 CO2

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3. Krebs Cycle (Citric Acid 3. Krebs Cycle (Citric Acid Cycle)Cycle) Total net yield (2 turns2 turns of krebs

cycle)

1. 2 - ATP (substrate-level phosphorylation)2. 6 - NADH3. 2 - FADH2

4. 4 - CO2

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4. Electron Transport Chain (ETC) 4. Electron Transport Chain (ETC) andand Oxidative Phosphorylation Oxidative Phosphorylation ((ChemiosmosisChemiosmosis))

Location:Location: inner mitochondrial membrane.

Uses ETC (cytochrome proteins)ETC (cytochrome proteins) and ATP Synthase (enzyme) to make ATP.

ETC pumps H+ (protons) across innermembrane (lowers pH in innermembrane space).Inner

MitochondrialMembrane

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4. Electron Transport Chain (ETC) 4. Electron Transport Chain (ETC) andand Oxidative Phosphorylation Oxidative Phosphorylation ((ChemiosmosisChemiosmosis))

The H+ then move via diffusiondiffusion (Proton Motive Force) through ATP Synthase to make ATP.

All NADH and FADH2 converted to ATP during this stage of cellular respirationcellular respiration.

Each NADH converts to 3 ATP. Each FADH2 converts to 2 ATP (enters the

ETC at a lower level than NADH).

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4. Electron Transport Chain (ETC) 4. Electron Transport Chain (ETC) andand Oxidative Phosphorylation Oxidative Phosphorylation ((ChemiosmosisChemiosmosis))

Innermembrane

Outermembrane

Innermembrane space

MatrixCristae

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4. 4. ETC and Oxidative Phosphorylation ETC and Oxidative Phosphorylation ((Chemiosmosis for Chemiosmosis for NADHNADH))

NADH+ H+

ATPSynthase

1H+ 2H+ 3H+

higher Hhigher H++

concentrationconcentration

H+

ADP + ATP

lower Hlower H++

concentrationconcentration

H+

(Proton Pumping)

P

E T C

NAD+2H+ + 1/2O2 H2O

Intermembrane SpaceIntermembrane Space

MatrixMatrix

InnerMitochondrialMembrane

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4. 4. ETC ETC and and Oxidative Phosphorylation Oxidative Phosphorylation (Chemiosmosis for (Chemiosmosis for FADH2)

FADH2

+ H+

ATPSynthase

1H+ 2H+

higher Hhigher H++

concentrationconcentration

H+

ADP + ATP

lower Hlower H++

concentrationconcentration

H+

(Proton Pumping)

P

E T C

FAD+ 2H+ +

1/2O2H2O

Intermembrane SpaceIntermembrane Space

MatrixMatrix

InnerMitochondrialMembrane

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TOTAL ATP YIELDTOTAL ATP YIELD1. 04 ATP - substrate-level

phosphorylation2. 34 ATP - ETC & oxidative

phosphorylation 38 ATP - TOTAL YIELD

ATPATP

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Eukaryotes(Have Membranes)

Total ATP Yield02 ATP - glycolysis (substrate-level phosphorylation)

04 ATP - converted from 2 NADH - glycolysis06 ATP - converted from 2 NADH - grooming

phase02 ATP - Krebs cycle (substrate-level phosphorylation)

18 ATP - converted from 6 NADH - Krebs cycle04 ATP - converted from 2 FADH2 - Krebs cycle36 ATP - TOTAL

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Maximum ATP Yield for Cellular Respiration (Eukaryotes)

36 ATP (maximum per glucose)

Glucose

Glycolysis

2ATP 4ATP 6ATP 18ATP 4ATP 2ATP

2 ATP(substrate-levelphosphorylation)

2NADH

2NADH6NADH

KrebsCycle

2FADH2

2 ATP(substrate-levelphosphorylation)

2 Pyruvate2 Acetyl CoA

ETC and Oxidative Phosphorylation

CytosolMitochondria

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ProkaryotesProkaryotes(Lack Membranes)(Lack Membranes)

Total ATP Yield02 ATP - glycolysis (substrate-level phosphorylation)

06 ATP - converted from 2 NADH - glycolysis06 ATP - converted from 2 NADH - grooming

phase02 ATP - Krebs cycle (substrate-level phosphorylation)

18 ATP - converted from 6 NADH - Krebs cycle04 ATP - converted from 2 FADH2 - Krebs cycle38 ATP - TOTAL

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Question:Question: In addition to glucose, what other In addition to glucose, what other

various food molecules are use in various food molecules are use in Cellular Respiration?Cellular Respiration?

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Catabolism of VariousCatabolism of VariousFood MoleculesFood Molecules Other organic molecules used for

fuel.

1. Carbohydrates: polysaccharides

2. Fats: glycerol’s and fatty acids3. Proteins: amino acids

If O2 is present after glycolysis this is made.

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29%

19%

43%

10%

1. ATP2. Lactic Acid3. Acetyl CoA4. Pyruvate

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20

21 22 23 24 25 26 27 28 29 30 31 32 33 34

The Krebs cycle makes CO2, FADH2 and:

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ATP and O2

ATP and NADH

NADH and O2

Pyruvate and O...

17%8%

0%

75%

1. ATP and O2

2. ATP and NADH3. NADH and O2

4. Pyruvate and O2

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20

21 22 23 24 25 26 27 28 29 30 31 32 33 34

What enzyme makes ATP in the ETC

56201 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20

21 22 23 24 25 26 27 28 29 30 31 32 33 34

1. ETC synthase2. ATP synthase3. Enolase4. Hexokinase

Why do protons (H+) move across the inner membrane

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Osmosis

Electr

on Flow

Diffusion

Active

transp

o...

8% 8%

65%

19%

1. Osmosis2. Electron Flow3. Diffusion4. Active transport

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20

21 22 23 24 25 26 27 28 29 30 31 32 33 34

Each NADH yields _______ ATP

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0%

16%

60%

24%

1. 12. 23. 34. 4

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20

21 22 23 24 25 26 27 28 29 30 31 32 33 34

Each FADH2 yields ____ATP

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1 2 3 4

0% 4%0%

96%1. 12. 23. 34. 4

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20

21 22 23 24 25 26 27 28 29 30 31 32 33 34

How many ATP are made in cellular respiration?

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11%4%

85%

0%

1. 42. 303. 384. 44

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20

21 22 23 24 25 26 27 28 29 30 31 32 33 34

Which process produces more ATP?

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Substr

ate leve...

Oxidative phos..

.

74%

26%

1. Substrate level phosphorylation

2. Oxidative phosphorylation

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20

21 22 23 24 25 26 27 28 29 30 31 32 33 34

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