BSC1010C Final Lab Practical Review
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BSC1010C Final Lab Practical Review
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What was the substratein the enzyme experiment?
Hydrogen Peroxide (H2O2)
What was the enzyme in the enzyme experiment?
catalase
What was the source of thisenzyme in the enzyme lab?
Sheep’s blood
What was/were the product(s)in the enzyme experiment?
Water and oxygen gas
What was the optimum pHfor the enzyme?
pH 7
Why is the enzyme less active at very high or very low pH?
Enzyme is denatured and reaction stops
What was the optimum temperaturefor the enzyme experiment?
40 C
Why do you see little gas produced at low temperatures?
Enzyme and substrate molecules are moving very slowly, so less enzyme-substrate collisions,
So less reactions
Why do you see little or no gas production at high temperatures?
The enzyme, which is a protein, is denaturedat high temperatures and reaction stops.
What did we measure to determinethe rate of the enzyme reaction?
Oxygen gas pressure – rate of oxygen gas production
What was the control in the enzyme experiment?
Distilled water in place of enzyme
What was the inhibitor used in enzyme experiment?
Copper sulfate (CuSO4)
What should happen when the inhibitor is added in the enzyme experiment?
Reaction should stop as the inhibitor is preventing the substrate and enzyme from coming together
What happened to the enzyme reaction whenthe temperature was raised to 60 C?
Reaction stopped because enzymewas denatured
What is this plant?
Coleus
What is the name of the pigmentin these leaves that is water-soluble?
What color is this pigment?
Anthocyaninred
What pigment is alcohol-soluble?What color is this pigment?
Chlorophyllgreen
What was used to indirectly detectphotosynthesis in these leaves?
IKI
What areas of the leaf carried out photosynthesis according to IKI test?
Green areas (where chlorophyll was located)
What is the name of the techniquethat uses this setup?
chromatography
What was this setup used toseparate?
Plant pigments
What is the genus of plant used to get these pigments from the leaves?
Magnolia
Name 3 things that determine the movement of the pigments up the paper.
1. Size of pigment molecule (molecular weight)2. Solubility of pigment in the solvent
3. H bonding of pigment to paper
What causes the solvent tomove up the paper?
Capillary action (adhesion + cohesion)
What is used to set 100%in absorption spectrum experiment?
acetone
What is the equation forcell respiration? (balanced, please)
C6H12O6 + 6 O2 6 CO2 + 6 H2O
What is the balanced equationfor photosynthesis?
6 CO2 + 6 H2O C6H12O6 + 6 O2
Given cell respiration and photosynthesis,which happens at all times?
Cell respiration
Given cell respiration and photosynthesis, which happens only in the light?
photosynthesis
Where is photosynthesis occurringin the following graph?
A and C
(Photosynthesis is taking in CO2 so level falls)
A B C D
What happens to CO2 levels in the respirometerin the light? WHY?
Decline Because photosynthesis is taking in CO2.
What happens to CO2 levels in the respirometerin the dark? WHY?
They go up.Photosynthesis is NOT occurring so only cell
respiration which is producing CO2
What genus of plant was used in the cell respiration – photosynthesis experiment?
Viburnum
What is the purpose of the beaker of water that was placed between the light source and the leaves?
To act as a heat sink (absorb the heat given off by the light)
What are the 2 typesof nuclear division?
Mitosis and Meiosis
In mitosis, how many cells are formed?What is their chromosome #?
2 cells
Diploid number
In meiosis, how many cells are formed?What is their chromosome #?
4 cells
Haploid #
What is the name of this region?
Root cap
What is the name of this region?
Region of maturation or differentiation
What is the name of this region?
Region ofelongation
What is the name of this region?
Apical meristemOr
Region of cell division
What is this stage? Is it a plant or animal cell?
Anaphase
Plant cell
What stage is this?What is arrow pointing to?
Telophase
Cleavage furrow
What is this phase?Plant or animal cell?
Prophase
Plant cell
What is this phase?Plant or animal cell?
Metaphase
Plant cell
What is this phase?What is arrow pointing to?
Telophase
Cell plate of plant cell
What is this phase?Plant or animal cell?
Anaphase
Animal cell
What is this phase of meiosis?
Metaphase I
What is this phase of meiosis?
Anaphase I
What is this phase of meiosis?
Telophase I
What specimen was used to study mitosisin animals?
Whitefish blastula
What specimen was used to studymitosis in plants?
Onion root tip
What cell division process occursin somatic cells?
mitosis
What cell division process producesgametes and spores?
meiosis
What cell division process is precededby S in the cell cycle?
Both Mitosis and Meiosis I
How many chromosomes are in the cell below?
2
What is this phase of meiosis?
Prophase I
What are the monomers that make up DNA?
nucleotides
If a piece of DNA has 4 sites recognized by the enzyme, how many fragments will be produced?
5
Enzymes that cut up DNA are called ___.
Restriction enzymesOr
Restriction endonucleases
Name an example of these enzymes.
EcoRI
HindIII
What produces the enzymes that cut up DNA?Why?
Bacteria
To cut up invading phage (bacterial viruses)
What is the source of the DNA used in this experiment?
Bacteriophage lambda
What is this piece of equipment?
micropipet
What is the volume this is set to measure(specify units)?
50 L
What is the purpose of the 2 “stop points”?
1st Stop – take up the liquid from tube2nd Step – dispense the liquid into new tube
Side blue button = ejects the tip
What technique is used to separatethe cut up pieces (fragments) of DNA?
electrophoresis
What is the name of the gel thatis used to support the fragments during
this separation?
agarose
Convert 0.6 mL to L
600 L
What is the size (in bp) of this fragment?
6,527 bp
Given the following fragments:500 bp 5,000 bp
2500 bp 25,000 bp Which fragment in the gel below is
the 25,000 bp fragment?
A
A B C D
What is the charge on DNA?
What gives it this charge?
Negative
The phosphate group
What are the places where the enzyme cuts DNA called?
Restriction sites
A piece of DNA was cut by 3 different enzymes. The standard is in well A. How many “cuts” were made by the enzyme in well B?
Two cuts
A B C D
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