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Biological Monitoring in Hiatt and Lick Runs Opequon Creek Watershed Frederick County Virginia
2008 Baseline Data and a Monitoring Framework
An Investigation by Marie R Dahl Woodward S Bousquet L Brandon Millholland J W Pangle Sean G Robertson Jeremy D Tovar Jared B Davis and Andrew G M Fisher
May 2009 (online edition July 2010)
Environmental Studies Department Shenandoah University Winchester Virginia 22601
i
DISCLAIMER
The information presented herein is intended for reference purposes in decision-making and as baseline data and research guidelines for future studies The interpretations of the data do not represent the only interpretations possible from this investigation Any opinions stated are not necessarily those of Shenandoah University its administration or its Board of Trustees
ii
Biological Monitoring in Hiatt and Lick Runs Opequon Creek Watershed Frederick County Virginia
2008 Baseline Data and a Monitoring Framework
An Investigation by
Marie R Dahl Woodward S Bousquet L Brandon Millholland J W Pangle Sean G Robertson Jeremy D Tovar
Jared B Davis and Andrew G M Fisher
Submitted by the Student Authors in Partial Fulfillment of the Requirements for
Environmental Measurement Methods (ES 421) Research Project in Environmental Studies (ES 492) andor
Research Project in Biology (BIO 492)
Technical Report No 3 Environmental Studies Department College of Arts and Sciences
Shenandoah University 1460 University Drive Winchester Virginia 22601
May 2009 Online Edition July 2010
iii
This report may be cited as follows Dahl Marie R Woodward S Bousquet L Brandon Millholland J W Pangle Sean G Robertson Jeremy D Tovar Jared B Davis and Andrew G M Fisher 2009 (online edition 2010) Biological Monitoring in Hiatt and Lick Runs Opequon Creek Watershed Frederick County Virginia 2008 Baseline Data and a Monitoring Framework Winchester Virginia Environmental Studies Program Shenandoah University
iv
ACKNOWLEDGEMENTS
Many individuals and organizations provided time support and expertise for this research
study Although we cannot list everyone by name we would like to express our sincere gratitude to all who made the project possible including Jim Lawrence ndash Coordinator Opequon Targeted Watershed Project Property owners along Hiatt Run and Lick Run
Mike Hoffman Leslie Johns The McCann-Slaughter Family Ervin and Barbara Simons Tonie Wallace-Aitken and Greg Aitken Historic Jordan Springs Event and Cultural Centre
Billy van Wart ndash Regional Aquatic Biologist Virginia Department of Environmental Quality Heather Kepple ndash Technician Opequon Targeted Watershed Project Field Assistance Andrew Barrett
Ben Bousquet Katherine McCann
Financial Support
Opequon Creek Targeted Watershed Grant Dr and Mrs Charles Leeper An anonymous family foundation Shenandoah University
v
vi
TABLE OF CONTENTS page
Disclaimer ii Title Page iii Acknowledgements v Table of Contents vii SECTION 1 OVERVIEW AND FINDINGS 1 Introduction 1 Methods and Analysis 3 Findings 3 Future Research 5 SECTION 2 RESULTS DATA TABLES 7 Site HLR-1 Mouth 7 Site HLR-2 Package Plant 8 Site HLR-3 Route 664 Bridge 9 Site HLR-4 Unnamed Tributary 10 Site HLR-5 Lick Run 11 Site HLR-6 McCann Farm 12 REFERENCES 13 APPENDICES 15 Appendix A Field Data Sheets Appendix B Water Quality Analysis through the Virginia Stream Condition Index (VSCI) Appendix C Laboratory Bench Sheets Note Photographs of the six sampling sites appear on a CD provided separately to the grant administrator
vii
viii
Section 1
OVERVIEW AND FINDINGS
Introduction
This report presents baseline water quality data for Hiatt Run and Lick Run -- termed the Hiatt-Lick Run subwatershed in this study Located in Frederick County Virginia Hiatt and Lick Runs are tributaries of Opequon Creek The Opequon in turn empties into the Potomac River and then the Chesapeake Bay The investigation was conducted by a Shenandoah University research team in 2008 Researchers used benthic macroinvertebrates (BMIs) as biological indicators of water quality This report presents an overview of the study followed by summary tables and a list of references The Appendix contains data sheets and a description of the data analysis These data will form part of the baseline water quality assessment that is being undertaken through the Opequon Creek Targeted Watershed Grant The grant administered by Jim Lawrence reflects a broad-based partnership that includes Virginia Tech Shenandoah University the Frederick-Winchester Service Authority The Opequon Watershed (TOW) citizens organization as well as federal state and local governments community groups and business interests Grant partners will use best management practices (BMPs) to reduce nutrient runoff and address other pollution problems in the Opequon Creek watershed Since 1996 Opequon Creek has appeared on the impaired waters list prepared by the Virginia Department of Environmental Quality (DEQ) The Hiatt-Lick Run subwatershed was selected for this study because it represents changes that are typical of the Opequon Creek watershed These changes include new commercial and residential developments as well as the implementation of water quality improvement practices such as tree planting in riparian zones fences to prevent livestock from entering streams and replacing outdated sewage treatment facilities such as a package plant on lower Hiatt Run
Seven students from Shenandoah Universityrsquos Environmental Studies Department conducted this study from May through December 2008 under the direction of Woodward Bousquet Professor of Environmental Studies and Biology The research team followed procedures described in the Rapid Bioassessment Protocols (RBPs) of the US Environmental Protection Agency (Barbour et al 1999) and the Virginia Stream Condition Index (VSCI) The VSCI provides an evaluation of water quality based on a streamrsquos benthic macroinvertebrate community (Burton and Gerritsen 2003 DEQ 2006b and 2008)
In consultation with Billy van Wart of the DEQ and grant administrator Jim Lawrence the Shenandoah University researchers selected six representative sampling sites in the Hiatt-Lick Run subwatershed Each site was given a name and an HLR (for Hiatt-Lick Run) code Figure 1 maps their positions while their characteristics and coordinates appear in the field data sheets (see Appendix A) These sites were established according to EPA and DEQ guidelines that generally call for a 100-meter-long stream reach with at least three riffle zones a width sufficient to accommodate a 1-meter seine and a substrate composed of least 35 cobbles
Specific locations of the sampling sites on Hiatt and Lick Runs were selected to reflect different watershed positions and changing land uses See Figure 1 Further information about each sampling site appears in the field data sheets (Appendix A)
1
Figure 1 Sampling Sites in the Hiatt-Lick Run Subwatershed of Opequon Creek Frederick County Virginia Scale 1 centimeter = 500 meters
HLR-1 (Mouth) measures water quality at the lowest point of the subwatershed as Hiatt Run flows into Opequon Creek Forests cover the immediate riparian zones on both sides of Hiatt Run This site will be used in future years by the DEQ for BMI monitoring HLR-2 (Package Plant) is situated on Hiatt Run just below the outflow pipe of an outdated package plant that formerly treated sewage from the Jordan Springs area During the study period the package plant was taken off-line and a pumping station was constructed The Frederick-Winchester Service Authority now treats this sewage at its Opequon Water Reclamation Facility HLR-3 (Route 664 Bridge) was chosen because it is located upstream from the package plant but downstream of the confluence of Hiatt Run and Lick Run HLR-4 (Unnamed Tributary) is located downstream of the Snowden Bridge residential development which has been under construction The area immediately upstream of this sampling site was being graded by heavy equipment during the May 2008 sampling time HLR-5 (Lick Run) the researchers learned dries up in late summer It had a modest but constant flow during when the May 2008 sample was taken HLR-6 (McCann Farm) represents the headwater areas of the Hiatt-Lick Run subwatershed of Opequon Creek The McCanns who graze cattle in their pastures that surround Hiatt Run here have been working with the Potomac Conservancy to implement Best Management Practices
2
(BMPs) to improve water quality These practices include fencing cattle out of the stream and spring runs and planting more trees in the riparian zone
Methods and Analysis
The research team established a sampling plan and then sampled according to methods the DEQ developed for the VSCI Using a 1 meter x 1 meter seine samples from 2m2 of substrate in riffle zones were to be collected twice at each site once in May 2008 and once in October 2008 At five of the six sites stream characteristics permitted the standard single-habitat sampling procedure At the McCann Farm (Site HLR-6) researchers needed to use multi-habitat sampling See Barbour et al (1999 pp 7-3 ff) for further descriptions of these two methods
All six sites were sampled in May 2008 Low-water conditions in October 2008 prevented the Shenandoah University group from collecting BMIs at Lick Run (HLR-5) that month but the other five sites were sampled successfully As a result the research team obtained eleven samples for analysis in this study Samples were preserved in ethanol for subsequent identification
DEQ procedures require subsampling when the number of BMIs in a given sample exceeds 100 All eleven samples in this study met that criterion Consequently researchers used a Caton tray to produce random subsamples of 90 to 110 specimens from each sample During the analysis that followed some specimens in the subsamples were rejected either because they turned out to be terrestrial species or because they were in the wrong life stage (eg pupae) In most cases the researchers identified specimens to the family level Primary taxonomic references were A Guide to Common Freshwater Invertebrates of North America (Voshell 2002) Fresh-Water Invertebrates of the United States (Pennak 1953) and An Introduction to the Aquatic Insects of North America (Merritt and Cummins 1996) DEQ Regional Aquatic Biologist Billy van Wart reviewed verified and where necessary corrected the identifications
The investigators then used the Virginia Stream Condition Index (VSCI) to produce water quality scores based on the BMIs collected A VSCI score is based on eight different measurement formulas called metrics Each metric is a numeric measure of a biological communityrsquos species composition diversity ecological niches or tolerance to pollution When combined into a single multimetric VSCI score the response of the benthic community to water conditions can be evaluated For further information and worksheets see Appendices B and C respectively
Findings
Table 1 presents the VSCI scores for the eleven samples collected in the Hiatt-Lick Run subwatershed in 2008 Since a sample year consists of two samples the mean scores for each site for the entire year are also included As the table shows
bull None of the 11 samples was rated excellent bull Only 1 of the 11 samples was rated as good bull Only 2 of the 11 samples were rated fair (ie in the ldquogrey zonerdquo between impaired and
unimpaired) bull The remaining 8 samples were in either moderately stressed or severely stressed
condition
3
Site Name and Code Sampling
Date of
TaxaVCSI Score
Water Quality Rating
Mouth (HLR-1) 21 May 2008 13 571 Fair (Gray Zone)
Mouth (HLR-1) 10 Oct 2008 10 608 Fair (Gray Zone)
site average for 2008 590 Fair (Gray Zone)
Package Plant (HLR-2) 29 May 2008 5 370 Severely Stressed
Package Plant (HLR-2) 10 Oct 2008 7 674 Good
site average for 2008 522 Moderately Stressed
Route 664 Bridge (HLR-3) 28 May 2008 9 485 Moderately Stressed
Route 664 Bridge (HLR-3) 10 Oct 2008 7 469 Moderately Stressed
site average for 2008 477 Moderately Stressed
Unnamed Tributary (HLR-4) 31 May 2008 7 173 Severely Stressed
Unnamed Tributary (HLR-4) 10 Oct 2008 17 476 Moderately Stressed
site average for 2008 325 Severely Stressed
Upper Lick Run (HLR-5) 29 May 2008 7 455 Moderately Stressed
site average for 2008 455 Moderately Stressed
McCann Farm (HLR-6) 30 May 2008 3 190 Severely Stressed
McCann Farm (HLR-6) 10 Oct 2008 10 316 Severely Stressed
site average for 2008 253 Severely Stressed
Summary 6 sites 11 samples
36 BMI taxa
VSCI = Virginia Stream Condition Index see Burton and Gerritsen (2003) and Virginia Department of Environmental Quality (2006b and 2008) Table 1 Summary Table of Virginia Stream Condition Index (VSCI) Scores and Water Quality Ratings for Sites in the Hiatt-Lick Run Subwatershed of Opequon Creek Frederick County Virginia 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
4
Improvements in water quality ratings at three sites between May and October suggest that BMPs and other changes may already be having some positive effects on water quality At the Package Plant (Site HLR-2) the VSCI score rose dramatically from 370 (severely stressed) to 674 (good) The small-scale sewage treatment plant as noted above was taken off-line during this period Similarly water quality at the Unnamed Tributary (HLR-4) increased from 173 (severely stressed) to 476 (moderately stressed) Adjacent construction was completed over the summer and grass was planted on the property Management practices on the McCann Farm (HLR-6) may be responsible for improving conditions for aquatic life in Hiatt Runrsquos headwaters Even though the water quality was still rated as severely stressed in October the VSCI score rose from 190 to 316 at this sampling site Further studies are needed to determine whether the changes noted in 2008 persist into the future In 2003 the DEQ began a Total Maximum Daily Load (TMDL) process for Opequon Creek as required by the Water Quality Act of 1987 The TMDL approach establishes a ldquopollution budgetrdquo for each impaired stream for each type of impairment and for each responsible pollutant In conjunction with the DEQ and the Virginia Department of Conservation and Recreation (DCR) Virginia Polytechnic Institutersquos Department of Biosystems Engineering developed a TMDL implementation plan (Opequon Creek IP Steering Committee et al 2006) Monitoring the BMI communities of Opequon Creek and its tributaries is essential to evaluating the progress of the proposed water quality improvement projects
Future Research The relatively small Hiatt-Lick Run subwatershed provides several case studies that can help assess the effectiveness of various water quality management practices not only in Opequon Creek but also in other watersheds in the Chesapeake Bay region and beyond In supporting that goal this study makes two principal contributions 1 It provides baseline data to measure the improvement or degradation of the Hiatt-Lick Run section of the Opequon Creek watershed and 2 It establishes a monitoring framework for the long-term biological monitoring of water quality and the evaluation of best management practices (BMPs) This framework consists of
bull Sampling Sites ndash six stream reaches in the Hiatt-Lick Run subwatershed selected as sampling sites according to EPA and DEQ specifications
bull Data Collection Protocols ndash based on the EPArsquos Rapid Bioassessment Protocols (RBPs)
(Barbour et al 1999) and the DEQrsquos Virginia Stream Index (VSCI) (Burton and Gerritsen 2003 DEQ 2006b and 2008) and
bull Analytical Procedures ndash contained in the Virginia Stream Condition Index (VSCI see
Burton and Gerritsen 2003 DEQ 2006b pp 8 and 33 and DEQ 2008 pp 29-34)
5
6
Section 2
RESULTS DATA TABLES
Site HLR-1 Mouth
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 13 189 6872 EPT taxa 5 117 4273 Ephemeroptera 121 477 2544 Plec +Tric less Hydropsych 154 562 2745 Scrapers 352 467 7536 Chironomidae 286 16 7267 Top 2 Dominant 550 328 6708 Hilsenhoff Biotic Index (HBI) 45 29 775VSCI Score 571
According to the DEQrsquos water quality standards aquatic life at this site is fair (gray zone) See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 2 VSCI Scores for Mouth (HLR-1) Site in Hiatt-Lick Run Sampled on 21 May 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 10 189 5292 EPT taxa 4 117 3423 Ephemeroptera 163 477 3424 Plec +Tric less Hydropsych 360 562 6415 Scrapers 221 467 4736 Chironomidae 00 16 10007 Top 2 Dominant 546 328 6768 Hilsenhoff Biotic Index (HBI) 39 29 859VSCI Score 608
According to the DEQrsquos water quality standards aquatic life at this site is fair (gray zone) See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 3 VSCI Scores for Mouth (HLR-1) Site in Hiatt-Lick Run Sampled 10 October 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
7
Site HLR-2 Package Plant
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 5 189 2642 EPT taxa 2 117 1713 Ephemeroptera 00 477 004 Plec +Tric less Hydropsych 110 562 1965 Scrapers 173 467 3706 Chironomidae 395 16 6157 Top 2 Dominant 568 328 6438 Hilsenhoff Biotic Index (HBI) 50 29 704VSCI Score 370
According to the DEQrsquos water quality standards aquatic life at this site is severely stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 4 VSCI Scores for Package Plant (HLR-2) Site in Hiatt-Lick Run Sampled 29 May 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 7 189 3702 EPT taxa 34 117 2563 Ephemeroptera 392 477 8214 Plec +Tric less Hydropsych 275 562 4895 Scrapers 539 467 10006 Chironomidae 00 16 10007 Top 2 Dominant 667 328 4968 Hilsenhoff Biotic Index (HBI) 32 29 958VSCI Score 674
According to the DEQrsquos water quality standards aquatic life at this site is good See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 5 VSCI Scores for Package Plant (HLR-2) Site in Hiatt-Lick Run Sampled 10 October 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
8
Site HLR-3 Route 664 Bridge
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 9 189 4762 EPT taxa 5 117 4273 Ephemeroptera 30 477 634 Plec +Tric less Hydropsych 61 562 1095 Scrapers 446 467 9556 Chironomidae 308 16 7037 Top 2 Dominant 723 328 4128 Hilsenhoff Biotic Index (HBI) 48 29 732VSCI Score 485
According to the DEQrsquos water quality standards aquatic life at this site is moderately stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 6 VSCI Scores for Route 664 Bridge (HLR-3) Site in Hiatt-Lick Run Sampled 28 May 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 7 189 3702 EPT taxa 4 117 3423 Ephemeroptera 40 477 844 Plec +Tric less Hydropsych 367 562 6535 Scrapers 122 467 2616 Chironomidae 00 16 10007 Top 2 Dominant 847 328 2288 Hilsenhoff Biotic Index (HBI) 42 29 817VSCI Score 469
According to the DEQrsquos water quality standards aquatic life at this site is moderately stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 7 VSCI Scores for Route 664 Bridge (HLR-3) Site in Hiatt-Lick Run Sampled 10 October 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
9
Site HLR-4 Unnamed Tributary
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 7 189 3702 EPT taxa 1 117 853 Ephemeroptera 00 477 004 Plec +Tric less Hydropsych 28 562 495 Scrapers 00 467 006 Chironomidae 847 16 1557 Top 2 Dominant 889 328 1658 Hilsenhoff Biotic Index (HBI) 60 29 563VSCI Score 173
According to the DEQrsquos water quality standards aquatic life at this site is severely stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 8 VSCI Scores for Unnamed Tributary (HLR-4) Site in Hiatt-Lick Run Sampled 31 May 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 17 189 8992 EPT taxa 3 117 2563 Ephemeroptera 16 477 344 Plec +Tric less Hydropsych 63 562 1125 Scrapers 48 467 1036 Chironomidae 127 16 8877 Top 2 Dominant 254 328 10008 Hilsenhoff Biotic Index (HBI) 63 29 521VSCI Score 476
According to the DEQrsquos water quality standards aquatic life at this site is moderately stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 9 VSCI Scores for Unnamed Tributary (HLR-4) Site in Hiatt-Lick Run Sampled 10 October 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
10
Site HLR-5 Lick Run
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 7 189 3702 EPT taxa 3 117 2563 Ephemeroptera 167 477 3504 Plec +Tric less Hydropsych 167 562 2975 Scrapers 106 467 2276 Chironomidae 348 16 6637 Top 2 Dominant 545 328 6778 Hilsenhoff Biotic Index (HBI) 43 29 803VSCI Score 455
According to the DEQrsquos water quality standards aquatic life at this site is moderately stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 10 VSCI Scores for Lick Run (HLR-5) Site in Hiatt-Lick Run Sampled 29 May 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
Note
Water conditions at the Lick Run (HLR-5) site were too low in October 2008 to permit a sample to be taken
11
Site HLR-6 McCann Farm
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 3 189 1592 EPT taxa 0 117 003 Ephemeroptera 00 477 004 Plec +Tric less Hydropsych 00 562 005 Scrapers 23 467 496 Chironomidae 00 16 10007 Top 2 Dominant 988 328 188 Hilsenhoff Biotic Index (HBI) 79 29 296VSCI Score 190
According to the DEQrsquos water quality standards aquatic life at this site is severely stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 11 VSCI Scores for McCann Farm (HLR-6) Site in Hiatt-Lick Run Sampled 30 May 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 10 189 5292 EPT taxa 3 117 2563 Ephemeroptera 51 477 1074 Plec +Tric less Hydropsych 09 562 165 Scrapers 43 467 926 Chironomidae 00 16 10007 Top 2 Dominant 887 328 1688 Hilsenhoff Biotic Index (HBI) 75 29 352VSCI Score 315
According to the DEQrsquos water quality standards aquatic life at this site is severely stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 12 VSCI Scores for McCann Farm (HLR-6) Site in Hiatt-Lick Run Sampled 10 October 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
12
REFERENCES
Barbour Michael T Jeroen Gerritsen Blaine D Snyder and James B Stribling 1999 Rapid bioassessment protocols for use in streams and wadeable rivers periphyton benthic macroinvertebrates and fish Second edition EPA 841-B-99-002 Washington DC US Environmental Protection Agency Office of Water Available online at httpwwwepagovOWOWmonitoringbioasspshtml Burton June and Jeroen Gerritsen 2003 A stream condition index for Virginia non-coastal streams Prepared for the US EPA Office of Science and Technology US EPA Region 3 Environmental Services Division and Virginia Department of Environmental Quality Owings Mills Maryland Tetra Tech Inc Available online at httpwwwdeqvirginiagovwatermonitoringpdf vastrmconpdf Hessler Rachel A Andrea R Martin Heidi D Ruggeri William B Seymour Jr and Woodward S Bousquet 2006 Water quality assessment using benthic macroinvertebrates A study and compilation Winchester Virginia Environmental Studies Program Shenandoah University Occasional publication no 10 Igoe Robert 2006 DEQ identifies nine impaired streams in Clarke Frederick The Winchester Star 15 July B1 Jackson Charlie 2005 Survey targets Opequon cleanup The Winchester Star 19 August B1 Johnston Elizabeth A and Woodward S Bousquet 2003 What happens when it rains A study of water quality in Abrams Creek and Town Run Winchester and Frederick County Virginia Winchester Virginia Environmental Studies Program Shenandoah University Occasional publication no7 Merritt Richard W and Kenneth W Cummins 1996 An introduction to the aquatic insects of North America Dubuque Iowa KendallHunt Publishing Company Opequon Creek IP Steering Committee Virginia Tech Department of Biological Systems Engineering and Center for TMDL and Watershed Studies University of Virginia Institute for Environmental Negotiation and West Virginia University Division of Resource Management 2006 Opequon Creek watershed TMDL implementation plan Blacksburg Virginia Virginia Tech Department of Biological Systems Engineering Available online at httpwwwtmdlbsevteduoutreachC82 Pennak Robert W 1953 Fresh-water invertebrates of the United States New York New York The Ronald Press Company Rosenburg David M and Vincent H Resh 1993 Freshwater biomonitoring and benthic macroinvertebrates New York New York Chapman amp Hall Shenandoah University Public Relations Office 2002 Data collected by SU and its partners helps identify impaired water Winchester Virginia Available online at httpwwwsuedutemp_ newscfmurlnum=32 Thorp James H and Alan P Covich 1991 Ecology and classification of North American freshwater invertebrates San Diego California Academic Press Inc
13
US Environmental Protection Agency 1997 Volunteer stream monitoring a methods manual EPA 841-B-97-003 Washington DC US Environmental Protection Agency Office of Water Van Meter Val 2003 Opequon Creek pollution limits to be established The Winchester Star 4 April B1 Virginia Department of Environmental Quality 2006a Virginia 305(b)303(d) water quality integrated report to Congress and the EPA Administrator for the period January 1 2000 to December 31 2004 Richmond Virginia Virginia Department of Environmental Quality Available online at httpwwwdeqvirginiagovwqair2006html __________ 2006b Using probabilistic data to validate the non-coastal Virginia Stream Condition Index (draft) VDEQ technical bulletin WQA2006-001 Virginia Department of Environmental Quality Available online at httpwwwloudounwatershedorgstream_assessmentreference_documents VA_DEQ_SCI_Validationpdf __________ 2008 Biological monitoring program quality assurance project plan for wadeable streams and rivers Richmond Virginia Virginia Department of Environmental Quality Update available online at httpwwwdeqstatevausbiomonitoringdocumentsBioMonQAPP_13Aug2008pdf Virginia Tech Aquatic Entomology Laboratory 2005 Taxa dictionary [Excel file] Blacksburg Virginia Virginia Tech Department of Biological Systems Engineering 2003a Opequon watershed TMDLs for benthic impairments Abrams Creek and Lower Opequon Creek Frederick and Clarke Counties Virginia Prepared for the Virginia Department of Environmental Quality and Virginia Department of Conservation and Recreation Blacksburg Virginia Available online at httpwwwdeqstate vaustmdlapptmdlsshenrvrabropebcpdf Voshell J Reese Jr 2002 A guide to common freshwater invertebrates of North America Blacksburg Virginia The McDonald amp Woodward Publishing Company
14
APPENDICES
15
16
a
Appendix A
FIELD DATA SHEETS
This appendix presents a Site Characterization Field Data Sheet for each of the six sampling reaches established on Hiatt Run and Lick Run for this study They were filled out during the sampling runs made in May 2008
These forms were adapted by Shenandoah University from forms used by the EPA and Virginia DEQ for recording characteristics of sampling sites
b
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Mouth STATION _HLR-1_____ STREAM CLASS LAT N 39 12749 (upstream) LONG W 078 04209(upstream) LAT N 39 12729 (downstream) LONG W 078 04148 (downstream) FORM COMPILED BY Jeremy Tovar RIVER BASIN Potomac River INVESTIGATORS JPangle B Millholland MDahl SRobertson W Bousquet JTovar BBousquet
DATE May 21 2008_________ TIME_250 PM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature __24___degC showers (intermittent) Comments _______________________
___ cloud cover _0_ ______________________________
X clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x ( Significant springs andor seeps) x (Valley ) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS
c
d
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence x FieldPasture Industrial Obvious sources Agricultural Other ____________ Stream scour steep slope erosion x Residential Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left Field and young thin deciduous forest 100 Bank=Grass cobble and shrub Right 90 maturing deciduous forest 45 shale slope Bank=Bedrock soil and herbs Indicate the dominant vegetation type(s) and record the dominant species
x Trees Shrubs x (Grasses) Forbs Dominant species Oak species and mix deciduous
INSTREAM FEATURES
Measured reach length ___100__m Canopy Cover Estimated stream width _35__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area __350_m2
Estimated stream depth _008_m Proportion of Reach Represented by Stream High water mark _15_m Morphology Types Surface velocity (at thalweg) _05__msec x Riffle__75___ x Run__20___ x Pool__5___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___00__ m2 Density of LWD _-_ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __15_____ x Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present Algae
WATER QUALITY Temperature __19___degC Water Odors x NormalNone Sewage Specific Conductance - Petroleum Chemical Fishy Other___________________ Dissolved Oxygen - Water Surface Oils Slick Sheen Globs Flecks pH __-_ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used _____NA________ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic x None Relict shells Other______None_____ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 8 Detritus sticks wood coarse plant materials
0 Boulder gt256 mm (10rsquorsquo) 0
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 51 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 40 Sand 006-2 mm (gritty) 1 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University
SITE CHARACTERIZATION FIELD DATA SHEET FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Package Plant STATION HLR-2 STREAM CLASS LAT N 39 129580 (upstream) LONG W 078 04900 (upstream) LAT N 39 129581 (downstream) LONG W 078 04822 (downstream) FORM COMPILED BY JW Pangle RIVER BASIN Potomac River INVESTIGATORS JPangle JTovar MDahl B Millholland SRobertson W Bousquet
DATE__19 May 2008_ TIME__1240__ PM
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature _____degC showers (intermittent) Comments __Sunny Day_____________
_5__ cloud cover _0__ ______________________________
x clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x (Significant springs andor seeps) x (Valley) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS
e
f
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence FieldPasture Industrial Obvious sources Agricultural Other ____________ __Upstream Construction and Stream bank Scour Residential __________________________________ Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left Deciduous forest with thick herb and shrub layers bank= vegetation and rocks Right Deciduous forest with thick herb and shrub layers bank=vegetation soil and rocks Indicate the dominant vegetation type(s) and record the dominant species
x Trees x Shrubs Grasses Forbs Dominant species Black Walnut White Ash Hackberry and American Sycamore
INSTREAM FEATURES
Measured reach length 100 m Canopy Cover Estimated stream width _35__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area __350_m2
Estimated stream depth __15__m Proportion of Reach Represented by Stream High water mark __22___m Morphology Types Surface velocity (at thalweg) _035__msec x Riffle__30___ x Run__30___ x Pool__40___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___10____ m2 Density of LWD _______ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __30___ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present__________________________________________________________
WATER QUALITY Temperature __18___degC Water Odors x NormalNone Sewage Specific Conductance __-____ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ____-____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used _____NA____ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other___None_____ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach SubstrateType Characteristic
Composition in Sampling Reach
Bedrock 3 Detritus sticks wood coarse plant materials
2 Boulder gt256 mm (10rsquorsquo) 5
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 70 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 22 Sand 006-2 mm (gritty) 0 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiattrsquos Run LOCATION Rt 664 Bridge STATION HLR-3 STREAM CLASS LAT N 39 12934 (upstream) LONG W 078 05019 (upstream) LAT N 39 12958 (downstream) LONG W 078 04958 (downstream) FORM COMPILED BY Sean Robertson RIVER BASIN Potomac River INVESTIGATORS SRobertson BMillholland WBousquet MDahl JPangle
DATE 28 May 2008 TIME 1127 AM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality 2008
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature __14___degC showers (intermittent) x Trace Comments _______________________
_65 x cloud cover ___ ______________________________
x clearsunny __________________________________
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x (Significant springs andor seeps) x (Valley) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS
g
h
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence Some evidence x FieldPasture Industrial x Obvious sources Agricultural x Other Old Package Plant Downstream x Residential Bridge and Road Power line and Pump station Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left 70 field 30 shrubs and deciduous trees Bank=Soil and vegetation Right Maturing deciduous forest steep slope Bank=Shale Bedrock Indicate the dominant vegetation type(s) and record the dominant species
x Trees x Shrubs x Grasses Forbs Dominant species Sycamore and Red Oaks
INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width __51___m Fully open x Partly open Partly shaded Fully shaded Sampling reach area _510_m2
Estimated stream depth __135___m Proportion of Reach Represented by Stream High water mark __-___m Morphology Types Surface velocity (at thalweg) __20___msec x Riffle_40_ x Run_50_ x Pool 10 Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___0____ m2 Density of LWD ___-____ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation ___50____ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present Brown Algae
WATER QUALITY Temperature __17___degC Water Odors x NormalNone Sewage Specific Conductance ___-___ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ___-_____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used ____NA_______ Clear x Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other____None____ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 15 Detritus sticks wood coarse plant materials
0 Boulder gt256 mm (10rsquorsquo) 15
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 62 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 8 Sand 006-2 mm (gritty) 0 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Unnamed Tributary STATION HLR-4 STREAM CLASS LAT N 39 13035 (upstream) LONG W 078 05129 (upstream) LAT N 39 13000 (downstream) LONG W 078 05086 (downstream) FORM COMPILED BY Marie Dahl RIVER BASIN Potomac River INVESTIGATORS M Dahl HKepple SRobertson AFisher
DATE 31 May 2008 TIME 5 PM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality 2008
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) YES x NO
rain (steady rain) Air Temperature __22___degC showers (intermittent) Comments _______________________
_0__ cloud cover _0__ ______________________________
x clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area __-___ km2 mi2 Montane Significant springs andor seeps x Valley Marsh or swamp Montane amp Valley Mixture of origins Other ___________________
COMMENTS Snowden Bridge development (upstream) and sewer line
i
j
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence x FieldPasture Industrial Obvious sources Agricultural Other ____________ Construction upstream and adjacent x Residential Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left Forested with rocky banks field across silt fence and 2m tree strip Right Forested with some rocky banks and forest through the highway Indicate the dominant vegetation type(s) and record the dominant species
x Trees x Shrubs x Grasses Forbs Dominant species White Ash and Hackberry
INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width ___38__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area __380__m2
Estimated stream depth __12__cm Proportion of Reach Represented by Stream High water mark __2___m Morphology Types Surface velocity (at thalweg) __46___msec x Riffle__25___ x Run__70___ x Pool__5___ Channelized Yes x No Dam Present x Yes No
LARGE WOODY DEBRIS
LWD _______ m2 Density of LWD _______ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation ___NA____ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present___Algae___
WATER QUALITY Temperature __16___degC Water Odors x NormalNone Sewage Specific Conductance __-____ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ___-_____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used ____NA_____ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells x Other___Shale_______ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 0 Detritus sticks wood coarse plant materials
10 Boulder gt256 mm (10rsquorsquo) 5
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 45 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 30 Sand 006-2 mm (gritty) 0 Marl grey shell
fragments 0
Silt 0004-006 mm 20 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Lick Run STATION HLR-5 STREAM CLASS LAT N 39 127517 (upstream) LONG W 078 05685(upstream) LAT N 39 12784 (downstream) LONG W 078 05631 (downstream) FORM COMPILED BY B Millholland RIVER BASIN Potomac River INVESTIGATORS B Millholland JTovar SRobertson MDahl WBousquet JPangle
DATE 29 May 2008 TIME 1040 AM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project
k
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature __15___degC showers (intermittent) Comments _______________________
_5_ X cloud cover _0__ ______________________________
x clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane Significant springs andor seeps x Valley Marsh or swamp Montane amp Valley Mixture of origins Other ___________________
COMMENTS Lick Run appears to be intermittent and may be dry in Fall 2008
l
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence x FieldPasture Industrial Obvious sources Agricultural Other ____________ Adjacent logging stream bank scour Residential Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left 85 young deciduous forest 15 logged Bank=Soil vegetation and cobbles Right Young deciduous forest on 30 slope Bank= Soil vegetation and cobble Indicate the dominant vegetation type(s) and record the dominant species
x Trees Shrubs Grasses Forbs Dominant species Mixed Deciduous INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width __11__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area 110 m2
Estimated stream depth _009__m Proportion of Reach Represented by Stream High water mark __96___m Morphology Types Surface velocity (at thalweg) __01___msec x Riffle__30___ x Run__5___ x Pool__65___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___0____ m2 Density of LWD ___-____ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __70___ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present__________________________________________________________
WATER QUALITY Temperature __14___degC Water Odors x NormalNone Sewage Specific Conductance __-__ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ___-___ Water Surface Oils Slick Sheen Globs Flecks pH __-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used _____NA______ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other_____None_____
SEDIMENT AND SUBSTRATE
Odors Deposits Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other________________ x Other Slightly Salty Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 0 Detritus sticks wood coarse plant materials
5 Boulder gt256 mm (10rsquorsquo) 0
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 75 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 23 Sand 006-2 mm (gritty) 2 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION McCann Farm STATION HLR-6 STREAM CLASS LAT N 39 13076 (upstream) LONG W 078 06539 (upstream) LAT N 39 13063 (downstream) LONG W 078 06473 (downstream) FORM COMPILED BY MDahl RIVER BASIN Potomac River INVESTIGATORS MDahl JPangle SRobertson W Bousqet BMillholland JTovar H Kepple
DATE 30 May 2008 TIME 1050 AM (start)
PROJECT NAME
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) YES x NO
rain (steady rain) Air Temperature __18___degC showers (intermittent) Comments _______________________
_85_x cloud cover _10__ ______________________________
clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x (Significant springs andor seeps) x ( Valley) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS Multi-habitat sampling with D-frame nets and seine
m
n
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial x No evidence Some evidence x FieldPasture Industrial Obvious sources x Agricultural Other ____________ Minor stream bank scour Residential Local Watershed Erosion x None Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left 35 bank grasses scattered trees and some bushes Right High bank with grasses and scattered trees Indicate the dominant vegetation type(s) and record the dominant species
x Trees Shrubs Grasses Forbs Dominant species Black Walnut and Festuca elatior
INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width __2___m Fully open Partly open x Partly shaded Fully shaded Sampling reach area __200_m2
Estimated stream depth __03_m Proportion of Reach Represented by Stream High water mark __1___m Morphology Types Surface velocity (at thalweg) __166_msec x Riffle__0___ x Run__55___ x Pool__45___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___2____ m2 Density of LWD ___-____ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __20_____ x Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present ____Glyceria (grass)sp__________
WATER QUALITY Temperature __16___degC Water Odors x NormalNone Sewage Specific Conductance ___-___ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ____-____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used ______NA___ Clear x Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other__NONE__ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 0 Detritus sticks wood coarse plant materials
5 Boulder gt256 mm (10rsquorsquo) 0
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 27 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 38 Sand 006-2 mm (gritty) 5 Marl grey shell
fragments 0 (lt1)
Silt 0004-006 mm 25 Clay lt 0004 mm (slick) 5 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Appendix B
WATER QUALITY ANALYSIS THROUGH THE VIRGINIA
STREAM CONDITION INDEX (VSCI)
The Virginia Stream Condition Index (VSCI) relies on eight metrics to categorize stream health According to Burton and Gerritsen (2003 p 1-1) this index ldquohellip can be used as a primary indicator of ecosystem health and can identify impairment with respect to the reference (or natural) conditionrdquo These metrics are numeric measures of a biological communityrsquos species composition diversity ecological niches or tolerance to pollution
Table 13 on the next page describes each VSCI metric lists its formula and provides the best standard value (BSV) for that metric Based on a 100-point scale the BSV is the score that reflects optimum conditions for aquatic life ie the best water quality value possible (DEQ 2006b pp 8 33) The table also explains whether the score increases or decreases with environmental stress
As Table 13 indicates each of the eight VSCI metrics is calculated individually Each formula provides a unitless score based on a 100-point scale with 0 as the worst possible score and 100 as the best Any metric that provides a result above 100 is changed to 100 in order for the index to work properly After the eight metrics are calculated they are summed and averaged to provide a final VSCI score on a 100-point scale
Based on statistical analysis of Virginiarsquos streams and their BMI communities the DEQ (2006b p 8) currently uses the following tiers to interpret VSCI scores
VSCI Scores Aquatic Life Use
(ALI) Tiers
lt 42 severely stressed
42-55 moderately stressed
55-63 unknownfair (gray zone)
63-73 good
gt73 excellent
Section 2 of this report presents the results of calculations for the six BMI sampling sites the research team studied in the Hiatt-Lick Run subwatershed in 2008
o
p
1 Total taxa the number of distinct taxa in the sample measures the overall diversity of the macroinvertebrate assemblage The best standard value (BSV) is 189 Score = 100 x (X189) Total taxa decreases with stress 2 EPT taxa the sum of the distinct taxa in the orders Ephemeroptera (mayflies) Plecoptera (stoneflies) and Trichoptera (caddisflies) These orders are known to be sensitive to pollution The best standard value (BSV) is 117 Score = 100 x (X117) EPT taxa decreases with stress 3 Percent Ephemeroptera the percent abundance of individuals in the sample that belong to the order Ephemeroptera (mayflies) The best standard value (BSV) is 477 Score = 100 x (X477) Percent Ephemeroptera decreases with stress 4 Percent Plecoptera + Trichoptera less Hydropsychidae the percent abundance of individuals in the sample that belong to the orders Plecoptera (stoneflies) and Trichoptera (caddisflies) excluding the pollution-tolerant caddisfly family Hydropsychidae The best standard value (BSV) is 562 Score = 100 x (X562) Percent Plecoptera + Trichoptera less Hydropsychidae decreases with stress 5 Percent Scrapers the percent abundance of individuals in the sample whose primary functional mechanism for feeding is grazing on the substrate or periphyton (ie attached algae and associated material) The best standard value (BSV) is 467 Score = 100 x (X467) Percent scrapers decreases with stress 6 Percent Chironomidae the percent abundance of individuals in the sample that are Chironomidae (non-biting midge) larvae and pupae The best standard value (BSV) is 16 Score = 100 x [(100-X)(100-16)] Percent Chironomidae increases with stress 7 Percent Top Two Dominant the percent abundance of the two most frequent taxa present in the sample The best standard value (BSV) is 328 Score = 100 x [(100-X)(100-328)] The Percent Top 2 Dominant increases with stress 8 Hilsenhoff Biotic Index (HBI) an abundance-weighted average pollution tolerance of all the individuals within a sample keyed to the family level Tolerance ratings range from 1 (highly intolerant) to 10 (highly tolerant) The best standard value (BSV) is 29 Score = 100 x [(10-X)(10-29)] The Hilsenhoff Biotic Index (HBI) increases with stress ______________________________________________________________________________ Table 13 Description of Benthic Macroinvertebrate VSCI Metrics Includes responses (increases decreases) of each VSCI metric to degraded water quality conditions Adapted from Burton and Gerritsen (2003 pp 3-15 3-16 6-2) and DEQ (2008)
q
Appendix C
LABORATORY BENCH SHEETS
Shenandoah Universityrsquos Environmental Studies Department developed the Benthic Macroinvertebrate Laboratory Bench Sheet and VSCI Worksheet to record BMI specimen identifications and their VSCI classifications for each sample
This form is based in part on bench sheets developed by the EPA and Virginia DEQ for recording specimen identifications Additional columns on Shenandoah Universityrsquos forms for relative numbers and VSCI classifications make the calculation and verification of VSCI scores easier
Note the column for taxonomistrsquos initials Specimens were identified by students in Shenandoah Universityrsquos research group and then verified or where necessary changed by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality This external review helps assure the validity of the study Originals of these bench sheets with handwritten taxonomistsrsquo initials will be kept on file at Shenandoah Universityrsquos Environmental Studies Department for five years after the completion of this study
r
s
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-1 STATION NAME Mouth LAT N 39deg 12749rsquo (upstream) LONG W 078deg 04209rsquo (upstream) LAT N 39deg 12729rsquo (downstream) LONG W 078deg 04148rsquo (downstream) FORM COMPILED BY Jeremy Tovar W S Bousquet RIVER BASIN Potomac River INVESTIGATORS Marie Dahl Brandon Millholand J W Pangle Sean Robertson Jeremy Tovar Woodward Bousquet Ben Bousquet
Collection date 21 May 2008 Sheet completion date 12 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Hirudinea 3 33 JT BVW PR 8 SP Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Coenagrionidae 2 22 BVW PR 9 CR Ephemeroptera (mayflies) Caenidae 1 11 JT BVW CG 6 SP Ephemerllidae 4 44 JT BVW CG 2 CR Heptageniidae 5 55 JT BVW SC 3 CG Isonychiidae 1 11 BVW CF 3 CG Plecoptera (stoneflies) Perlidae 14 154 JT BVW PR 2 CR Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Corydalidae 1 11 JT BVW PR 5 CR Coleoptera (beetles) Elmidae 24 264 I JT BVW SC 4 CG Dytiscidae 4 44 JT BVW PR 6 GN Psephenidae 3 33 JT BVW SC 4 CG Diptera (true flies) Tipulidae 3 33 JT BVW SH 4 BU Chironomidae 26 286 JT BVW CG 6 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
t
Other Total Individuals 91 1001 Total Taxa 13
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
u
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-1 STATION NAME Mouth LAT N 39deg 12749rsquo (upstream) LONG W 078deg 04209rsquo (upstream) LAT N 39deg 12729rsquo (downstream) LONG W 078deg 04148rsquo (downstream) FORM COMPILED BY Jeremy Tovar W S Bousquet RIVER BASIN Potomac River INVESTIGATORS Brandon Millholand JW Pangle Jeremy Tovar Heather Kepple
Collection date 10 October 2008 Sheet completion date 12 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Oligochaeta 3 35 JT BVW CG 8 BU Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Coenagrionidae 2 23 JT BVW PR 9 CR Ephemeroptera (mayflies) Heptageniidae 12 140 JT BVW SC 3 CG Isonychiidae 2 23 BVW CF 3 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Hydropsychidae 16 186 JT BVW CF 6 CG Philopotamidae 31 360 JT BVW CF 2 CG Hemiptera (true bugs) (Veliidae) (5) - JT BVW ( ) Megaloptera (dobsonflies etc) Corydalidae 10 116 JT BVW PR 5 CR Coleoptera (beetles) Dytiscidae 1 12 JT BVW PR 6 GN Psephenidae 7 81 JT BVW SC 4 CG Diptera (true flies) Tipulidae 2 23 BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
v
Other Total Individuals 86
(91) 999
Total Taxa 10 (11)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009 Veliidae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
w
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-2 STATION NAME Package Plant LAT N 39deg 12958rsquo (upstream) LONG W 078deg 04900 (upstream) LAT N 39deg 12958rsquo (downstream) LONG W 078deg 04822 (downstream) FORM COMPILED BY JW Pangle W Bousquet RIVER BASIN Potomac River INVESTIGATORS JW Pangle S Robertson J Tovar M Dahl B Millholland W Bousquet
Collection date 29 May 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Plecoptera (stoneflies) Perlidae 11 136 I JP BVW PR 1 CR Trichoptera (caddisflies) Hydropsychidae 10 123 I JP BVW CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 14 173 I JP BVW SC 4 CG Diptera (true flies) Chironomidae 32 395 I JP BVW CG 6 BU Simuliidae 14 173 I JP BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
x
Other Total Individuals 81 1000 Total Taxa 5
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-2 STATION NAME Package Plant LAT N 39deg 12958rsquo (upstream) LONG W 078deg 04900 (upstream) LAT N 39deg 12958rsquo (downstream) LONG W 078deg 04822 (downstream) FORM COMPILED BY JW Pangle W Bousquet RIVER BASIN Potomac River INVESTIGATORS JW Pangle Brandon Millholland Jeremy Tovar Heather Kepple
Collection date 10 October 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Heptageniidae 40 392 I WBJP SC 3 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Hydropsychidae 7 69 I JP WB BVW CF 6 CG Philopotamidae 28 275 I JP WB BVW CF 2 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Corydalidae 6 59 I JP WB BVW PR 5 CR Coleoptera (beetles) Psephenidae 7 69 I JP WB BVW SC 4 CG Elmidae 8 78 I JP WB BVW SC 4 CG Diptera (true flies) Tipulidae 6 59 I JP WB BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
y
z
Other Total Individuals 102 1001 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-3 STATION NAME Rt 664 Bridge LAT N 39deg 12934rsquo (upstream) LONG W 078deg 05019rsquo (upstream) LAT N 39deg 12951rsquo (downstream) LONG W 078deg 04958rsquo (downstream) FORM COMPILED BY S Robertson W Bousquet RIVER BASIN Potomac River INVESTIGATORS SRobertson BMillholland WBousquet MDahl JPangle
Collection date 28 May 2008 Sheet completion date 11 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Leptophlebiidae 1 15 I SGR BVW CG 2 CR Baetidae 1 15 I BVW CG 5 CG Plecoptera (stoneflies) Perlidae 3 46 I SGR BVW PR 1 CR Nemouridae 1 15 I BVW SH 3 CR Trichoptera (caddisflies) Hydropsychidae 5 77 I SGR BVW CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 27 415 I SGR BVW SC 4 CG Psephenidae 2 31 A SGR BVW SC 4 CG Diptera (true flies) Simuliidae 5 77 I SGR BVW CF 6 CG Chironomidae 20 308 I SGR BVW CG 6 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
aa
bb
Other Total Individuals 65 999 Total Taxa 9
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-3 STATION NAME Rt 664 Bridge LAT N 39deg 12934rsquo (upstream) LONG W 078deg 05019rsquo (upstream) LAT N 39deg 12951rsquo (downstream) LONG W 078deg 04958rsquo (downstream) FORM COMPILED BY S Robertson RIVER BASIN Potomac River INVESTIGATORS S Robertson M Dahl A Fisher H Kepple
Collection date 10 Oct 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Heptageniidae 2 20 I SGR SC 3 CG Isonychiidae 2 20 I BVW CF 2 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Philopotamidae 36 367 I SGR CF 2 CG Hydropsychidae 47 480 I SGR CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 2 20 A SGR SC 4 CG Psephenidae 8 82 A SGR SC 4 CG Diptera (true flies) Tabanidae 1 10 I SGR PR 7 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
cc
dd
Other Total Individuals 98 999 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-4 STATION NAME Unnamed Tributary LAT (upstream) N 39deg 13035rsquo LONG (upstream) W 078deg 05129rsquo LAT (downstream) N 39deg 13000rsquo LONG (downstream) W 078deg 5086rsquo FORM COMPILED BY A Fisher M Dahl rev W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson A Fisher H Kepple
Collection date 31 May 2008 Sheet completion date rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Flatworms (Turbellaria) Worms (Oligochaeta) Naididae 1 14 - BVW CG 8 BU Leeches (Hirudinea) Snails (Gastropoda) Clams (Bivalvia) Isopods (Isopoda) Asellidae 3 42 A AF BVW CG 8 SP Scuds (Amphipoda) Crayfish Shrimp (Decapoda) Water Mites (Hydracarina) Dragonflies (Odonata Anisoptera) Damselflies (Odonata Zygoptera) Mayflies (Ephemeroptera) Stoneflies (Plecoptera) Perlidae 2 28 I AF BVW PR 1 CR Caddisflies (Trichoptera) True Bugs (Hemiptera) Corixidae 1 14 I AF BVW MP 9 DV Dobsonflies (Megaloptera) Beetles (Coleoptera) Dytiscidae 1 14 I AF BVW PR 6 GN True Flies (Diptera) Chironomidae 61 847 I AF BVW CG 6 BU Simuliidae 3 42 I AF BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
ee
ff
Other Total Individuals 72 1001 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-4 STATION NAME Unnamed Tributary LAT (upstream) N 39deg 13035rsquo LONG (upstream) W 078deg 05129rsquo LAT (downstream) N 39deg 13000rsquo LONG (downstream) W 078deg 5086rsquo FORM COMPILED BY Andrew G M Fisher W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson A Fisher H Kepple
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Flatworms (Turbellaria) Worms (Oligochaeta) Lumbriculidae 2 32 A AF BVW CG 8 BU Leeches (Hirudinea) Snails (Gastropoda) Lymnaeidae 1 16 A BVW CG 9 GN Clams (Bivalvia) Isopods (Isopoda) Scuds (Amphipoda) Crayfish Shrimp (Decapoda) Cambaridae 1 16 A AF BVW GN 5 GN Water Mites (Hydracarina) Dragonflies (Odonata Anisoptera) Damselflies (Odonata Zygoptera) Calopterygidae 4 63 I BVW PR 7 CL Mayflies (Ephemeroptera) Tricorythidae 1 16 I BVW CG 5 SP Stoneflies (Plecoptera) Caddisflies (Trichoptera) Phryganeidae 4 63 I AF BVW SH 4 CR Hydropsychidae 6 95 I AF BVW CF 6 CG I True Bugs (Hemiptera) Corixidae 8 127 I AF BVW MP 9 DV (Veliidae ) (7) - I AF BVW ( ) Dobsonflies (Megaloptera) Sialidae 3 48 I AF BVW PR 7 SP Corydalidae 2 32 I AF BVW PR 5 CR Beetles (Coleoptera) Haliplidae 1 16 A AF BVW MP 8 GN Dysticidae 7 111 I AF BVW PR 6 GN Hydrophilidae 1 16 A AF BVW PR 8 GN Elmidae 3 48 I BVW SC 4 CG True Flies (Diptera) Chironomidae 8 127 I AF BVW CG 6 BU Simuliidae 7 111 I AF BVW CF 6 CG Tipulidae 4 63 I AF BVW SH 4 GU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
gg
hh
Other Total Individuals 63
(70) 1000
Total Taxa 17 (18)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Veliidae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-5 STATION NAME Lick Run LAT N 39deg 12752rsquo (upstream) LONG W 078deg 05685rsquo (upstream) LAT N 39deg 12784rsquo (downstream) LONG W 078deg 05631rsquo (downstream) FORM COMPILED BY Brandon Millholland W Bousquet RIVER BASIN Potomac River INVESTIGATORS J Pangle S Robertson W Bousquet JTovar M Dahl
Collection date 29 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Gomphidae 1 15 I BVW PR 3 BU Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Leptophlebiidae 11 167 A LBM BVW CG 2 CR Plecoptera (stoneflies) Perlidae 10 152 A LBM BVW PR 1 CR Perlodiae 1 15 A LBM BVW PR 2 CR Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 5 76 A LBM BVW SC 4 CG Elmidae 2 30 I LBM BVW SC 4 CG Diptera (true flies) (Culicidae) (3) - P LMB BVW ( ) Chironomidae 23 348 I LBM BVW CG 6 BU Simuliidae 13 197 I LBM BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
ii
jj
Other Total Individuals 66
(69) 1000
Total Taxa 7 (8)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Culicidae (Diptera) pupae not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl J Pangle S Robertson W Bousquet B Millholland J Tovar H Kepple
Collection date 30 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 1 12 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Asellidae 114 965 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Plecoptera (stoneflies) Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 2 23 A MRD BVW SC 4 CG Diptera (true flies)
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
kk
ll
Other Total Individuals 117 1000 Total Taxa 3
Notes Subsample taken 29 October 2009 All taxonomic determinations by MRD were confirmed by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson K McCann A Fisher H Kepple A Barrett
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 2 17 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Sphaeriidae 2 17 I MRD BVW CF 8 BU Isopoda (isopods) Asellidae 99 853 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Calopterygidae 2 17 A MRD BVW PR 7 CL Ephemeroptera (mayflies) Leptophlebiidae 2 17 I MRD BVW CG 2 CR Heptageniidae 4 34 A MRD BVW SC 3 CG Plecoptera (stoneflies) Perlidae 1 09 A MRD BVW PR 1 CR Trichoptera (caddisflies) Hemiptera (true bugs) (Gerridae) (1) - A MRD BVW ( ) Corixidae 1 09 A MRD BVW PR 5 CR Megaloptera (dobsonflies etc) Coleoptera (beetles) Dryopidae 1 09 A MRD BVW SC 5 CG Diptera (true flies) Tipulidae 2 17 I BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
mm
nn
Other Total Individuals 116
(117) 999
Total Taxa 10 (11)
Notes Subsample taken 6 November 2008 Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009 Gerridae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
- Disclaimer ii
- Title Page iii
- Acknowledgements v
- Table of Contents vii
- SECTION 2 RESULTS DATA TABLES 7
- OVERVIEW AND FINDINGS
- Introduction
- RESULTS DATA TABLES
- REFERENCES
- FIELD DATA SHEETS
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- SubstrateType
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- WATER QUALITY ANALYSIS
- THROUGH THE VIRGINIA
- STREAM CONDITION INDEX (VSCI)
- LABORATORY BENCH SHEETS
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
DISCLAIMER
The information presented herein is intended for reference purposes in decision-making and as baseline data and research guidelines for future studies The interpretations of the data do not represent the only interpretations possible from this investigation Any opinions stated are not necessarily those of Shenandoah University its administration or its Board of Trustees
ii
Biological Monitoring in Hiatt and Lick Runs Opequon Creek Watershed Frederick County Virginia
2008 Baseline Data and a Monitoring Framework
An Investigation by
Marie R Dahl Woodward S Bousquet L Brandon Millholland J W Pangle Sean G Robertson Jeremy D Tovar
Jared B Davis and Andrew G M Fisher
Submitted by the Student Authors in Partial Fulfillment of the Requirements for
Environmental Measurement Methods (ES 421) Research Project in Environmental Studies (ES 492) andor
Research Project in Biology (BIO 492)
Technical Report No 3 Environmental Studies Department College of Arts and Sciences
Shenandoah University 1460 University Drive Winchester Virginia 22601
May 2009 Online Edition July 2010
iii
This report may be cited as follows Dahl Marie R Woodward S Bousquet L Brandon Millholland J W Pangle Sean G Robertson Jeremy D Tovar Jared B Davis and Andrew G M Fisher 2009 (online edition 2010) Biological Monitoring in Hiatt and Lick Runs Opequon Creek Watershed Frederick County Virginia 2008 Baseline Data and a Monitoring Framework Winchester Virginia Environmental Studies Program Shenandoah University
iv
ACKNOWLEDGEMENTS
Many individuals and organizations provided time support and expertise for this research
study Although we cannot list everyone by name we would like to express our sincere gratitude to all who made the project possible including Jim Lawrence ndash Coordinator Opequon Targeted Watershed Project Property owners along Hiatt Run and Lick Run
Mike Hoffman Leslie Johns The McCann-Slaughter Family Ervin and Barbara Simons Tonie Wallace-Aitken and Greg Aitken Historic Jordan Springs Event and Cultural Centre
Billy van Wart ndash Regional Aquatic Biologist Virginia Department of Environmental Quality Heather Kepple ndash Technician Opequon Targeted Watershed Project Field Assistance Andrew Barrett
Ben Bousquet Katherine McCann
Financial Support
Opequon Creek Targeted Watershed Grant Dr and Mrs Charles Leeper An anonymous family foundation Shenandoah University
v
vi
TABLE OF CONTENTS page
Disclaimer ii Title Page iii Acknowledgements v Table of Contents vii SECTION 1 OVERVIEW AND FINDINGS 1 Introduction 1 Methods and Analysis 3 Findings 3 Future Research 5 SECTION 2 RESULTS DATA TABLES 7 Site HLR-1 Mouth 7 Site HLR-2 Package Plant 8 Site HLR-3 Route 664 Bridge 9 Site HLR-4 Unnamed Tributary 10 Site HLR-5 Lick Run 11 Site HLR-6 McCann Farm 12 REFERENCES 13 APPENDICES 15 Appendix A Field Data Sheets Appendix B Water Quality Analysis through the Virginia Stream Condition Index (VSCI) Appendix C Laboratory Bench Sheets Note Photographs of the six sampling sites appear on a CD provided separately to the grant administrator
vii
viii
Section 1
OVERVIEW AND FINDINGS
Introduction
This report presents baseline water quality data for Hiatt Run and Lick Run -- termed the Hiatt-Lick Run subwatershed in this study Located in Frederick County Virginia Hiatt and Lick Runs are tributaries of Opequon Creek The Opequon in turn empties into the Potomac River and then the Chesapeake Bay The investigation was conducted by a Shenandoah University research team in 2008 Researchers used benthic macroinvertebrates (BMIs) as biological indicators of water quality This report presents an overview of the study followed by summary tables and a list of references The Appendix contains data sheets and a description of the data analysis These data will form part of the baseline water quality assessment that is being undertaken through the Opequon Creek Targeted Watershed Grant The grant administered by Jim Lawrence reflects a broad-based partnership that includes Virginia Tech Shenandoah University the Frederick-Winchester Service Authority The Opequon Watershed (TOW) citizens organization as well as federal state and local governments community groups and business interests Grant partners will use best management practices (BMPs) to reduce nutrient runoff and address other pollution problems in the Opequon Creek watershed Since 1996 Opequon Creek has appeared on the impaired waters list prepared by the Virginia Department of Environmental Quality (DEQ) The Hiatt-Lick Run subwatershed was selected for this study because it represents changes that are typical of the Opequon Creek watershed These changes include new commercial and residential developments as well as the implementation of water quality improvement practices such as tree planting in riparian zones fences to prevent livestock from entering streams and replacing outdated sewage treatment facilities such as a package plant on lower Hiatt Run
Seven students from Shenandoah Universityrsquos Environmental Studies Department conducted this study from May through December 2008 under the direction of Woodward Bousquet Professor of Environmental Studies and Biology The research team followed procedures described in the Rapid Bioassessment Protocols (RBPs) of the US Environmental Protection Agency (Barbour et al 1999) and the Virginia Stream Condition Index (VSCI) The VSCI provides an evaluation of water quality based on a streamrsquos benthic macroinvertebrate community (Burton and Gerritsen 2003 DEQ 2006b and 2008)
In consultation with Billy van Wart of the DEQ and grant administrator Jim Lawrence the Shenandoah University researchers selected six representative sampling sites in the Hiatt-Lick Run subwatershed Each site was given a name and an HLR (for Hiatt-Lick Run) code Figure 1 maps their positions while their characteristics and coordinates appear in the field data sheets (see Appendix A) These sites were established according to EPA and DEQ guidelines that generally call for a 100-meter-long stream reach with at least three riffle zones a width sufficient to accommodate a 1-meter seine and a substrate composed of least 35 cobbles
Specific locations of the sampling sites on Hiatt and Lick Runs were selected to reflect different watershed positions and changing land uses See Figure 1 Further information about each sampling site appears in the field data sheets (Appendix A)
1
Figure 1 Sampling Sites in the Hiatt-Lick Run Subwatershed of Opequon Creek Frederick County Virginia Scale 1 centimeter = 500 meters
HLR-1 (Mouth) measures water quality at the lowest point of the subwatershed as Hiatt Run flows into Opequon Creek Forests cover the immediate riparian zones on both sides of Hiatt Run This site will be used in future years by the DEQ for BMI monitoring HLR-2 (Package Plant) is situated on Hiatt Run just below the outflow pipe of an outdated package plant that formerly treated sewage from the Jordan Springs area During the study period the package plant was taken off-line and a pumping station was constructed The Frederick-Winchester Service Authority now treats this sewage at its Opequon Water Reclamation Facility HLR-3 (Route 664 Bridge) was chosen because it is located upstream from the package plant but downstream of the confluence of Hiatt Run and Lick Run HLR-4 (Unnamed Tributary) is located downstream of the Snowden Bridge residential development which has been under construction The area immediately upstream of this sampling site was being graded by heavy equipment during the May 2008 sampling time HLR-5 (Lick Run) the researchers learned dries up in late summer It had a modest but constant flow during when the May 2008 sample was taken HLR-6 (McCann Farm) represents the headwater areas of the Hiatt-Lick Run subwatershed of Opequon Creek The McCanns who graze cattle in their pastures that surround Hiatt Run here have been working with the Potomac Conservancy to implement Best Management Practices
2
(BMPs) to improve water quality These practices include fencing cattle out of the stream and spring runs and planting more trees in the riparian zone
Methods and Analysis
The research team established a sampling plan and then sampled according to methods the DEQ developed for the VSCI Using a 1 meter x 1 meter seine samples from 2m2 of substrate in riffle zones were to be collected twice at each site once in May 2008 and once in October 2008 At five of the six sites stream characteristics permitted the standard single-habitat sampling procedure At the McCann Farm (Site HLR-6) researchers needed to use multi-habitat sampling See Barbour et al (1999 pp 7-3 ff) for further descriptions of these two methods
All six sites were sampled in May 2008 Low-water conditions in October 2008 prevented the Shenandoah University group from collecting BMIs at Lick Run (HLR-5) that month but the other five sites were sampled successfully As a result the research team obtained eleven samples for analysis in this study Samples were preserved in ethanol for subsequent identification
DEQ procedures require subsampling when the number of BMIs in a given sample exceeds 100 All eleven samples in this study met that criterion Consequently researchers used a Caton tray to produce random subsamples of 90 to 110 specimens from each sample During the analysis that followed some specimens in the subsamples were rejected either because they turned out to be terrestrial species or because they were in the wrong life stage (eg pupae) In most cases the researchers identified specimens to the family level Primary taxonomic references were A Guide to Common Freshwater Invertebrates of North America (Voshell 2002) Fresh-Water Invertebrates of the United States (Pennak 1953) and An Introduction to the Aquatic Insects of North America (Merritt and Cummins 1996) DEQ Regional Aquatic Biologist Billy van Wart reviewed verified and where necessary corrected the identifications
The investigators then used the Virginia Stream Condition Index (VSCI) to produce water quality scores based on the BMIs collected A VSCI score is based on eight different measurement formulas called metrics Each metric is a numeric measure of a biological communityrsquos species composition diversity ecological niches or tolerance to pollution When combined into a single multimetric VSCI score the response of the benthic community to water conditions can be evaluated For further information and worksheets see Appendices B and C respectively
Findings
Table 1 presents the VSCI scores for the eleven samples collected in the Hiatt-Lick Run subwatershed in 2008 Since a sample year consists of two samples the mean scores for each site for the entire year are also included As the table shows
bull None of the 11 samples was rated excellent bull Only 1 of the 11 samples was rated as good bull Only 2 of the 11 samples were rated fair (ie in the ldquogrey zonerdquo between impaired and
unimpaired) bull The remaining 8 samples were in either moderately stressed or severely stressed
condition
3
Site Name and Code Sampling
Date of
TaxaVCSI Score
Water Quality Rating
Mouth (HLR-1) 21 May 2008 13 571 Fair (Gray Zone)
Mouth (HLR-1) 10 Oct 2008 10 608 Fair (Gray Zone)
site average for 2008 590 Fair (Gray Zone)
Package Plant (HLR-2) 29 May 2008 5 370 Severely Stressed
Package Plant (HLR-2) 10 Oct 2008 7 674 Good
site average for 2008 522 Moderately Stressed
Route 664 Bridge (HLR-3) 28 May 2008 9 485 Moderately Stressed
Route 664 Bridge (HLR-3) 10 Oct 2008 7 469 Moderately Stressed
site average for 2008 477 Moderately Stressed
Unnamed Tributary (HLR-4) 31 May 2008 7 173 Severely Stressed
Unnamed Tributary (HLR-4) 10 Oct 2008 17 476 Moderately Stressed
site average for 2008 325 Severely Stressed
Upper Lick Run (HLR-5) 29 May 2008 7 455 Moderately Stressed
site average for 2008 455 Moderately Stressed
McCann Farm (HLR-6) 30 May 2008 3 190 Severely Stressed
McCann Farm (HLR-6) 10 Oct 2008 10 316 Severely Stressed
site average for 2008 253 Severely Stressed
Summary 6 sites 11 samples
36 BMI taxa
VSCI = Virginia Stream Condition Index see Burton and Gerritsen (2003) and Virginia Department of Environmental Quality (2006b and 2008) Table 1 Summary Table of Virginia Stream Condition Index (VSCI) Scores and Water Quality Ratings for Sites in the Hiatt-Lick Run Subwatershed of Opequon Creek Frederick County Virginia 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
4
Improvements in water quality ratings at three sites between May and October suggest that BMPs and other changes may already be having some positive effects on water quality At the Package Plant (Site HLR-2) the VSCI score rose dramatically from 370 (severely stressed) to 674 (good) The small-scale sewage treatment plant as noted above was taken off-line during this period Similarly water quality at the Unnamed Tributary (HLR-4) increased from 173 (severely stressed) to 476 (moderately stressed) Adjacent construction was completed over the summer and grass was planted on the property Management practices on the McCann Farm (HLR-6) may be responsible for improving conditions for aquatic life in Hiatt Runrsquos headwaters Even though the water quality was still rated as severely stressed in October the VSCI score rose from 190 to 316 at this sampling site Further studies are needed to determine whether the changes noted in 2008 persist into the future In 2003 the DEQ began a Total Maximum Daily Load (TMDL) process for Opequon Creek as required by the Water Quality Act of 1987 The TMDL approach establishes a ldquopollution budgetrdquo for each impaired stream for each type of impairment and for each responsible pollutant In conjunction with the DEQ and the Virginia Department of Conservation and Recreation (DCR) Virginia Polytechnic Institutersquos Department of Biosystems Engineering developed a TMDL implementation plan (Opequon Creek IP Steering Committee et al 2006) Monitoring the BMI communities of Opequon Creek and its tributaries is essential to evaluating the progress of the proposed water quality improvement projects
Future Research The relatively small Hiatt-Lick Run subwatershed provides several case studies that can help assess the effectiveness of various water quality management practices not only in Opequon Creek but also in other watersheds in the Chesapeake Bay region and beyond In supporting that goal this study makes two principal contributions 1 It provides baseline data to measure the improvement or degradation of the Hiatt-Lick Run section of the Opequon Creek watershed and 2 It establishes a monitoring framework for the long-term biological monitoring of water quality and the evaluation of best management practices (BMPs) This framework consists of
bull Sampling Sites ndash six stream reaches in the Hiatt-Lick Run subwatershed selected as sampling sites according to EPA and DEQ specifications
bull Data Collection Protocols ndash based on the EPArsquos Rapid Bioassessment Protocols (RBPs)
(Barbour et al 1999) and the DEQrsquos Virginia Stream Index (VSCI) (Burton and Gerritsen 2003 DEQ 2006b and 2008) and
bull Analytical Procedures ndash contained in the Virginia Stream Condition Index (VSCI see
Burton and Gerritsen 2003 DEQ 2006b pp 8 and 33 and DEQ 2008 pp 29-34)
5
6
Section 2
RESULTS DATA TABLES
Site HLR-1 Mouth
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 13 189 6872 EPT taxa 5 117 4273 Ephemeroptera 121 477 2544 Plec +Tric less Hydropsych 154 562 2745 Scrapers 352 467 7536 Chironomidae 286 16 7267 Top 2 Dominant 550 328 6708 Hilsenhoff Biotic Index (HBI) 45 29 775VSCI Score 571
According to the DEQrsquos water quality standards aquatic life at this site is fair (gray zone) See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 2 VSCI Scores for Mouth (HLR-1) Site in Hiatt-Lick Run Sampled on 21 May 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 10 189 5292 EPT taxa 4 117 3423 Ephemeroptera 163 477 3424 Plec +Tric less Hydropsych 360 562 6415 Scrapers 221 467 4736 Chironomidae 00 16 10007 Top 2 Dominant 546 328 6768 Hilsenhoff Biotic Index (HBI) 39 29 859VSCI Score 608
According to the DEQrsquos water quality standards aquatic life at this site is fair (gray zone) See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 3 VSCI Scores for Mouth (HLR-1) Site in Hiatt-Lick Run Sampled 10 October 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
7
Site HLR-2 Package Plant
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 5 189 2642 EPT taxa 2 117 1713 Ephemeroptera 00 477 004 Plec +Tric less Hydropsych 110 562 1965 Scrapers 173 467 3706 Chironomidae 395 16 6157 Top 2 Dominant 568 328 6438 Hilsenhoff Biotic Index (HBI) 50 29 704VSCI Score 370
According to the DEQrsquos water quality standards aquatic life at this site is severely stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 4 VSCI Scores for Package Plant (HLR-2) Site in Hiatt-Lick Run Sampled 29 May 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 7 189 3702 EPT taxa 34 117 2563 Ephemeroptera 392 477 8214 Plec +Tric less Hydropsych 275 562 4895 Scrapers 539 467 10006 Chironomidae 00 16 10007 Top 2 Dominant 667 328 4968 Hilsenhoff Biotic Index (HBI) 32 29 958VSCI Score 674
According to the DEQrsquos water quality standards aquatic life at this site is good See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 5 VSCI Scores for Package Plant (HLR-2) Site in Hiatt-Lick Run Sampled 10 October 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
8
Site HLR-3 Route 664 Bridge
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 9 189 4762 EPT taxa 5 117 4273 Ephemeroptera 30 477 634 Plec +Tric less Hydropsych 61 562 1095 Scrapers 446 467 9556 Chironomidae 308 16 7037 Top 2 Dominant 723 328 4128 Hilsenhoff Biotic Index (HBI) 48 29 732VSCI Score 485
According to the DEQrsquos water quality standards aquatic life at this site is moderately stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 6 VSCI Scores for Route 664 Bridge (HLR-3) Site in Hiatt-Lick Run Sampled 28 May 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 7 189 3702 EPT taxa 4 117 3423 Ephemeroptera 40 477 844 Plec +Tric less Hydropsych 367 562 6535 Scrapers 122 467 2616 Chironomidae 00 16 10007 Top 2 Dominant 847 328 2288 Hilsenhoff Biotic Index (HBI) 42 29 817VSCI Score 469
According to the DEQrsquos water quality standards aquatic life at this site is moderately stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 7 VSCI Scores for Route 664 Bridge (HLR-3) Site in Hiatt-Lick Run Sampled 10 October 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
9
Site HLR-4 Unnamed Tributary
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 7 189 3702 EPT taxa 1 117 853 Ephemeroptera 00 477 004 Plec +Tric less Hydropsych 28 562 495 Scrapers 00 467 006 Chironomidae 847 16 1557 Top 2 Dominant 889 328 1658 Hilsenhoff Biotic Index (HBI) 60 29 563VSCI Score 173
According to the DEQrsquos water quality standards aquatic life at this site is severely stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 8 VSCI Scores for Unnamed Tributary (HLR-4) Site in Hiatt-Lick Run Sampled 31 May 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 17 189 8992 EPT taxa 3 117 2563 Ephemeroptera 16 477 344 Plec +Tric less Hydropsych 63 562 1125 Scrapers 48 467 1036 Chironomidae 127 16 8877 Top 2 Dominant 254 328 10008 Hilsenhoff Biotic Index (HBI) 63 29 521VSCI Score 476
According to the DEQrsquos water quality standards aquatic life at this site is moderately stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 9 VSCI Scores for Unnamed Tributary (HLR-4) Site in Hiatt-Lick Run Sampled 10 October 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
10
Site HLR-5 Lick Run
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 7 189 3702 EPT taxa 3 117 2563 Ephemeroptera 167 477 3504 Plec +Tric less Hydropsych 167 562 2975 Scrapers 106 467 2276 Chironomidae 348 16 6637 Top 2 Dominant 545 328 6778 Hilsenhoff Biotic Index (HBI) 43 29 803VSCI Score 455
According to the DEQrsquos water quality standards aquatic life at this site is moderately stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 10 VSCI Scores for Lick Run (HLR-5) Site in Hiatt-Lick Run Sampled 29 May 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
Note
Water conditions at the Lick Run (HLR-5) site were too low in October 2008 to permit a sample to be taken
11
Site HLR-6 McCann Farm
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 3 189 1592 EPT taxa 0 117 003 Ephemeroptera 00 477 004 Plec +Tric less Hydropsych 00 562 005 Scrapers 23 467 496 Chironomidae 00 16 10007 Top 2 Dominant 988 328 188 Hilsenhoff Biotic Index (HBI) 79 29 296VSCI Score 190
According to the DEQrsquos water quality standards aquatic life at this site is severely stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 11 VSCI Scores for McCann Farm (HLR-6) Site in Hiatt-Lick Run Sampled 30 May 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 10 189 5292 EPT taxa 3 117 2563 Ephemeroptera 51 477 1074 Plec +Tric less Hydropsych 09 562 165 Scrapers 43 467 926 Chironomidae 00 16 10007 Top 2 Dominant 887 328 1688 Hilsenhoff Biotic Index (HBI) 75 29 352VSCI Score 315
According to the DEQrsquos water quality standards aquatic life at this site is severely stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 12 VSCI Scores for McCann Farm (HLR-6) Site in Hiatt-Lick Run Sampled 10 October 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
12
REFERENCES
Barbour Michael T Jeroen Gerritsen Blaine D Snyder and James B Stribling 1999 Rapid bioassessment protocols for use in streams and wadeable rivers periphyton benthic macroinvertebrates and fish Second edition EPA 841-B-99-002 Washington DC US Environmental Protection Agency Office of Water Available online at httpwwwepagovOWOWmonitoringbioasspshtml Burton June and Jeroen Gerritsen 2003 A stream condition index for Virginia non-coastal streams Prepared for the US EPA Office of Science and Technology US EPA Region 3 Environmental Services Division and Virginia Department of Environmental Quality Owings Mills Maryland Tetra Tech Inc Available online at httpwwwdeqvirginiagovwatermonitoringpdf vastrmconpdf Hessler Rachel A Andrea R Martin Heidi D Ruggeri William B Seymour Jr and Woodward S Bousquet 2006 Water quality assessment using benthic macroinvertebrates A study and compilation Winchester Virginia Environmental Studies Program Shenandoah University Occasional publication no 10 Igoe Robert 2006 DEQ identifies nine impaired streams in Clarke Frederick The Winchester Star 15 July B1 Jackson Charlie 2005 Survey targets Opequon cleanup The Winchester Star 19 August B1 Johnston Elizabeth A and Woodward S Bousquet 2003 What happens when it rains A study of water quality in Abrams Creek and Town Run Winchester and Frederick County Virginia Winchester Virginia Environmental Studies Program Shenandoah University Occasional publication no7 Merritt Richard W and Kenneth W Cummins 1996 An introduction to the aquatic insects of North America Dubuque Iowa KendallHunt Publishing Company Opequon Creek IP Steering Committee Virginia Tech Department of Biological Systems Engineering and Center for TMDL and Watershed Studies University of Virginia Institute for Environmental Negotiation and West Virginia University Division of Resource Management 2006 Opequon Creek watershed TMDL implementation plan Blacksburg Virginia Virginia Tech Department of Biological Systems Engineering Available online at httpwwwtmdlbsevteduoutreachC82 Pennak Robert W 1953 Fresh-water invertebrates of the United States New York New York The Ronald Press Company Rosenburg David M and Vincent H Resh 1993 Freshwater biomonitoring and benthic macroinvertebrates New York New York Chapman amp Hall Shenandoah University Public Relations Office 2002 Data collected by SU and its partners helps identify impaired water Winchester Virginia Available online at httpwwwsuedutemp_ newscfmurlnum=32 Thorp James H and Alan P Covich 1991 Ecology and classification of North American freshwater invertebrates San Diego California Academic Press Inc
13
US Environmental Protection Agency 1997 Volunteer stream monitoring a methods manual EPA 841-B-97-003 Washington DC US Environmental Protection Agency Office of Water Van Meter Val 2003 Opequon Creek pollution limits to be established The Winchester Star 4 April B1 Virginia Department of Environmental Quality 2006a Virginia 305(b)303(d) water quality integrated report to Congress and the EPA Administrator for the period January 1 2000 to December 31 2004 Richmond Virginia Virginia Department of Environmental Quality Available online at httpwwwdeqvirginiagovwqair2006html __________ 2006b Using probabilistic data to validate the non-coastal Virginia Stream Condition Index (draft) VDEQ technical bulletin WQA2006-001 Virginia Department of Environmental Quality Available online at httpwwwloudounwatershedorgstream_assessmentreference_documents VA_DEQ_SCI_Validationpdf __________ 2008 Biological monitoring program quality assurance project plan for wadeable streams and rivers Richmond Virginia Virginia Department of Environmental Quality Update available online at httpwwwdeqstatevausbiomonitoringdocumentsBioMonQAPP_13Aug2008pdf Virginia Tech Aquatic Entomology Laboratory 2005 Taxa dictionary [Excel file] Blacksburg Virginia Virginia Tech Department of Biological Systems Engineering 2003a Opequon watershed TMDLs for benthic impairments Abrams Creek and Lower Opequon Creek Frederick and Clarke Counties Virginia Prepared for the Virginia Department of Environmental Quality and Virginia Department of Conservation and Recreation Blacksburg Virginia Available online at httpwwwdeqstate vaustmdlapptmdlsshenrvrabropebcpdf Voshell J Reese Jr 2002 A guide to common freshwater invertebrates of North America Blacksburg Virginia The McDonald amp Woodward Publishing Company
14
APPENDICES
15
16
a
Appendix A
FIELD DATA SHEETS
This appendix presents a Site Characterization Field Data Sheet for each of the six sampling reaches established on Hiatt Run and Lick Run for this study They were filled out during the sampling runs made in May 2008
These forms were adapted by Shenandoah University from forms used by the EPA and Virginia DEQ for recording characteristics of sampling sites
b
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Mouth STATION _HLR-1_____ STREAM CLASS LAT N 39 12749 (upstream) LONG W 078 04209(upstream) LAT N 39 12729 (downstream) LONG W 078 04148 (downstream) FORM COMPILED BY Jeremy Tovar RIVER BASIN Potomac River INVESTIGATORS JPangle B Millholland MDahl SRobertson W Bousquet JTovar BBousquet
DATE May 21 2008_________ TIME_250 PM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature __24___degC showers (intermittent) Comments _______________________
___ cloud cover _0_ ______________________________
X clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x ( Significant springs andor seeps) x (Valley ) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS
c
d
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence x FieldPasture Industrial Obvious sources Agricultural Other ____________ Stream scour steep slope erosion x Residential Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left Field and young thin deciduous forest 100 Bank=Grass cobble and shrub Right 90 maturing deciduous forest 45 shale slope Bank=Bedrock soil and herbs Indicate the dominant vegetation type(s) and record the dominant species
x Trees Shrubs x (Grasses) Forbs Dominant species Oak species and mix deciduous
INSTREAM FEATURES
Measured reach length ___100__m Canopy Cover Estimated stream width _35__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area __350_m2
Estimated stream depth _008_m Proportion of Reach Represented by Stream High water mark _15_m Morphology Types Surface velocity (at thalweg) _05__msec x Riffle__75___ x Run__20___ x Pool__5___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___00__ m2 Density of LWD _-_ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __15_____ x Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present Algae
WATER QUALITY Temperature __19___degC Water Odors x NormalNone Sewage Specific Conductance - Petroleum Chemical Fishy Other___________________ Dissolved Oxygen - Water Surface Oils Slick Sheen Globs Flecks pH __-_ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used _____NA________ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic x None Relict shells Other______None_____ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 8 Detritus sticks wood coarse plant materials
0 Boulder gt256 mm (10rsquorsquo) 0
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 51 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 40 Sand 006-2 mm (gritty) 1 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University
SITE CHARACTERIZATION FIELD DATA SHEET FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Package Plant STATION HLR-2 STREAM CLASS LAT N 39 129580 (upstream) LONG W 078 04900 (upstream) LAT N 39 129581 (downstream) LONG W 078 04822 (downstream) FORM COMPILED BY JW Pangle RIVER BASIN Potomac River INVESTIGATORS JPangle JTovar MDahl B Millholland SRobertson W Bousquet
DATE__19 May 2008_ TIME__1240__ PM
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature _____degC showers (intermittent) Comments __Sunny Day_____________
_5__ cloud cover _0__ ______________________________
x clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x (Significant springs andor seeps) x (Valley) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS
e
f
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence FieldPasture Industrial Obvious sources Agricultural Other ____________ __Upstream Construction and Stream bank Scour Residential __________________________________ Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left Deciduous forest with thick herb and shrub layers bank= vegetation and rocks Right Deciduous forest with thick herb and shrub layers bank=vegetation soil and rocks Indicate the dominant vegetation type(s) and record the dominant species
x Trees x Shrubs Grasses Forbs Dominant species Black Walnut White Ash Hackberry and American Sycamore
INSTREAM FEATURES
Measured reach length 100 m Canopy Cover Estimated stream width _35__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area __350_m2
Estimated stream depth __15__m Proportion of Reach Represented by Stream High water mark __22___m Morphology Types Surface velocity (at thalweg) _035__msec x Riffle__30___ x Run__30___ x Pool__40___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___10____ m2 Density of LWD _______ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __30___ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present__________________________________________________________
WATER QUALITY Temperature __18___degC Water Odors x NormalNone Sewage Specific Conductance __-____ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ____-____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used _____NA____ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other___None_____ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach SubstrateType Characteristic
Composition in Sampling Reach
Bedrock 3 Detritus sticks wood coarse plant materials
2 Boulder gt256 mm (10rsquorsquo) 5
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 70 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 22 Sand 006-2 mm (gritty) 0 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiattrsquos Run LOCATION Rt 664 Bridge STATION HLR-3 STREAM CLASS LAT N 39 12934 (upstream) LONG W 078 05019 (upstream) LAT N 39 12958 (downstream) LONG W 078 04958 (downstream) FORM COMPILED BY Sean Robertson RIVER BASIN Potomac River INVESTIGATORS SRobertson BMillholland WBousquet MDahl JPangle
DATE 28 May 2008 TIME 1127 AM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality 2008
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature __14___degC showers (intermittent) x Trace Comments _______________________
_65 x cloud cover ___ ______________________________
x clearsunny __________________________________
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x (Significant springs andor seeps) x (Valley) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS
g
h
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence Some evidence x FieldPasture Industrial x Obvious sources Agricultural x Other Old Package Plant Downstream x Residential Bridge and Road Power line and Pump station Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left 70 field 30 shrubs and deciduous trees Bank=Soil and vegetation Right Maturing deciduous forest steep slope Bank=Shale Bedrock Indicate the dominant vegetation type(s) and record the dominant species
x Trees x Shrubs x Grasses Forbs Dominant species Sycamore and Red Oaks
INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width __51___m Fully open x Partly open Partly shaded Fully shaded Sampling reach area _510_m2
Estimated stream depth __135___m Proportion of Reach Represented by Stream High water mark __-___m Morphology Types Surface velocity (at thalweg) __20___msec x Riffle_40_ x Run_50_ x Pool 10 Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___0____ m2 Density of LWD ___-____ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation ___50____ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present Brown Algae
WATER QUALITY Temperature __17___degC Water Odors x NormalNone Sewage Specific Conductance ___-___ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ___-_____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used ____NA_______ Clear x Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other____None____ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 15 Detritus sticks wood coarse plant materials
0 Boulder gt256 mm (10rsquorsquo) 15
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 62 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 8 Sand 006-2 mm (gritty) 0 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Unnamed Tributary STATION HLR-4 STREAM CLASS LAT N 39 13035 (upstream) LONG W 078 05129 (upstream) LAT N 39 13000 (downstream) LONG W 078 05086 (downstream) FORM COMPILED BY Marie Dahl RIVER BASIN Potomac River INVESTIGATORS M Dahl HKepple SRobertson AFisher
DATE 31 May 2008 TIME 5 PM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality 2008
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) YES x NO
rain (steady rain) Air Temperature __22___degC showers (intermittent) Comments _______________________
_0__ cloud cover _0__ ______________________________
x clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area __-___ km2 mi2 Montane Significant springs andor seeps x Valley Marsh or swamp Montane amp Valley Mixture of origins Other ___________________
COMMENTS Snowden Bridge development (upstream) and sewer line
i
j
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence x FieldPasture Industrial Obvious sources Agricultural Other ____________ Construction upstream and adjacent x Residential Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left Forested with rocky banks field across silt fence and 2m tree strip Right Forested with some rocky banks and forest through the highway Indicate the dominant vegetation type(s) and record the dominant species
x Trees x Shrubs x Grasses Forbs Dominant species White Ash and Hackberry
INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width ___38__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area __380__m2
Estimated stream depth __12__cm Proportion of Reach Represented by Stream High water mark __2___m Morphology Types Surface velocity (at thalweg) __46___msec x Riffle__25___ x Run__70___ x Pool__5___ Channelized Yes x No Dam Present x Yes No
LARGE WOODY DEBRIS
LWD _______ m2 Density of LWD _______ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation ___NA____ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present___Algae___
WATER QUALITY Temperature __16___degC Water Odors x NormalNone Sewage Specific Conductance __-____ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ___-_____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used ____NA_____ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells x Other___Shale_______ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 0 Detritus sticks wood coarse plant materials
10 Boulder gt256 mm (10rsquorsquo) 5
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 45 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 30 Sand 006-2 mm (gritty) 0 Marl grey shell
fragments 0
Silt 0004-006 mm 20 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Lick Run STATION HLR-5 STREAM CLASS LAT N 39 127517 (upstream) LONG W 078 05685(upstream) LAT N 39 12784 (downstream) LONG W 078 05631 (downstream) FORM COMPILED BY B Millholland RIVER BASIN Potomac River INVESTIGATORS B Millholland JTovar SRobertson MDahl WBousquet JPangle
DATE 29 May 2008 TIME 1040 AM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project
k
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature __15___degC showers (intermittent) Comments _______________________
_5_ X cloud cover _0__ ______________________________
x clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane Significant springs andor seeps x Valley Marsh or swamp Montane amp Valley Mixture of origins Other ___________________
COMMENTS Lick Run appears to be intermittent and may be dry in Fall 2008
l
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence x FieldPasture Industrial Obvious sources Agricultural Other ____________ Adjacent logging stream bank scour Residential Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left 85 young deciduous forest 15 logged Bank=Soil vegetation and cobbles Right Young deciduous forest on 30 slope Bank= Soil vegetation and cobble Indicate the dominant vegetation type(s) and record the dominant species
x Trees Shrubs Grasses Forbs Dominant species Mixed Deciduous INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width __11__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area 110 m2
Estimated stream depth _009__m Proportion of Reach Represented by Stream High water mark __96___m Morphology Types Surface velocity (at thalweg) __01___msec x Riffle__30___ x Run__5___ x Pool__65___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___0____ m2 Density of LWD ___-____ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __70___ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present__________________________________________________________
WATER QUALITY Temperature __14___degC Water Odors x NormalNone Sewage Specific Conductance __-__ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ___-___ Water Surface Oils Slick Sheen Globs Flecks pH __-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used _____NA______ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other_____None_____
SEDIMENT AND SUBSTRATE
Odors Deposits Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other________________ x Other Slightly Salty Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 0 Detritus sticks wood coarse plant materials
5 Boulder gt256 mm (10rsquorsquo) 0
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 75 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 23 Sand 006-2 mm (gritty) 2 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION McCann Farm STATION HLR-6 STREAM CLASS LAT N 39 13076 (upstream) LONG W 078 06539 (upstream) LAT N 39 13063 (downstream) LONG W 078 06473 (downstream) FORM COMPILED BY MDahl RIVER BASIN Potomac River INVESTIGATORS MDahl JPangle SRobertson W Bousqet BMillholland JTovar H Kepple
DATE 30 May 2008 TIME 1050 AM (start)
PROJECT NAME
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) YES x NO
rain (steady rain) Air Temperature __18___degC showers (intermittent) Comments _______________________
_85_x cloud cover _10__ ______________________________
clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x (Significant springs andor seeps) x ( Valley) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS Multi-habitat sampling with D-frame nets and seine
m
n
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial x No evidence Some evidence x FieldPasture Industrial Obvious sources x Agricultural Other ____________ Minor stream bank scour Residential Local Watershed Erosion x None Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left 35 bank grasses scattered trees and some bushes Right High bank with grasses and scattered trees Indicate the dominant vegetation type(s) and record the dominant species
x Trees Shrubs Grasses Forbs Dominant species Black Walnut and Festuca elatior
INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width __2___m Fully open Partly open x Partly shaded Fully shaded Sampling reach area __200_m2
Estimated stream depth __03_m Proportion of Reach Represented by Stream High water mark __1___m Morphology Types Surface velocity (at thalweg) __166_msec x Riffle__0___ x Run__55___ x Pool__45___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___2____ m2 Density of LWD ___-____ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __20_____ x Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present ____Glyceria (grass)sp__________
WATER QUALITY Temperature __16___degC Water Odors x NormalNone Sewage Specific Conductance ___-___ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ____-____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used ______NA___ Clear x Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other__NONE__ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 0 Detritus sticks wood coarse plant materials
5 Boulder gt256 mm (10rsquorsquo) 0
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 27 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 38 Sand 006-2 mm (gritty) 5 Marl grey shell
fragments 0 (lt1)
Silt 0004-006 mm 25 Clay lt 0004 mm (slick) 5 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Appendix B
WATER QUALITY ANALYSIS THROUGH THE VIRGINIA
STREAM CONDITION INDEX (VSCI)
The Virginia Stream Condition Index (VSCI) relies on eight metrics to categorize stream health According to Burton and Gerritsen (2003 p 1-1) this index ldquohellip can be used as a primary indicator of ecosystem health and can identify impairment with respect to the reference (or natural) conditionrdquo These metrics are numeric measures of a biological communityrsquos species composition diversity ecological niches or tolerance to pollution
Table 13 on the next page describes each VSCI metric lists its formula and provides the best standard value (BSV) for that metric Based on a 100-point scale the BSV is the score that reflects optimum conditions for aquatic life ie the best water quality value possible (DEQ 2006b pp 8 33) The table also explains whether the score increases or decreases with environmental stress
As Table 13 indicates each of the eight VSCI metrics is calculated individually Each formula provides a unitless score based on a 100-point scale with 0 as the worst possible score and 100 as the best Any metric that provides a result above 100 is changed to 100 in order for the index to work properly After the eight metrics are calculated they are summed and averaged to provide a final VSCI score on a 100-point scale
Based on statistical analysis of Virginiarsquos streams and their BMI communities the DEQ (2006b p 8) currently uses the following tiers to interpret VSCI scores
VSCI Scores Aquatic Life Use
(ALI) Tiers
lt 42 severely stressed
42-55 moderately stressed
55-63 unknownfair (gray zone)
63-73 good
gt73 excellent
Section 2 of this report presents the results of calculations for the six BMI sampling sites the research team studied in the Hiatt-Lick Run subwatershed in 2008
o
p
1 Total taxa the number of distinct taxa in the sample measures the overall diversity of the macroinvertebrate assemblage The best standard value (BSV) is 189 Score = 100 x (X189) Total taxa decreases with stress 2 EPT taxa the sum of the distinct taxa in the orders Ephemeroptera (mayflies) Plecoptera (stoneflies) and Trichoptera (caddisflies) These orders are known to be sensitive to pollution The best standard value (BSV) is 117 Score = 100 x (X117) EPT taxa decreases with stress 3 Percent Ephemeroptera the percent abundance of individuals in the sample that belong to the order Ephemeroptera (mayflies) The best standard value (BSV) is 477 Score = 100 x (X477) Percent Ephemeroptera decreases with stress 4 Percent Plecoptera + Trichoptera less Hydropsychidae the percent abundance of individuals in the sample that belong to the orders Plecoptera (stoneflies) and Trichoptera (caddisflies) excluding the pollution-tolerant caddisfly family Hydropsychidae The best standard value (BSV) is 562 Score = 100 x (X562) Percent Plecoptera + Trichoptera less Hydropsychidae decreases with stress 5 Percent Scrapers the percent abundance of individuals in the sample whose primary functional mechanism for feeding is grazing on the substrate or periphyton (ie attached algae and associated material) The best standard value (BSV) is 467 Score = 100 x (X467) Percent scrapers decreases with stress 6 Percent Chironomidae the percent abundance of individuals in the sample that are Chironomidae (non-biting midge) larvae and pupae The best standard value (BSV) is 16 Score = 100 x [(100-X)(100-16)] Percent Chironomidae increases with stress 7 Percent Top Two Dominant the percent abundance of the two most frequent taxa present in the sample The best standard value (BSV) is 328 Score = 100 x [(100-X)(100-328)] The Percent Top 2 Dominant increases with stress 8 Hilsenhoff Biotic Index (HBI) an abundance-weighted average pollution tolerance of all the individuals within a sample keyed to the family level Tolerance ratings range from 1 (highly intolerant) to 10 (highly tolerant) The best standard value (BSV) is 29 Score = 100 x [(10-X)(10-29)] The Hilsenhoff Biotic Index (HBI) increases with stress ______________________________________________________________________________ Table 13 Description of Benthic Macroinvertebrate VSCI Metrics Includes responses (increases decreases) of each VSCI metric to degraded water quality conditions Adapted from Burton and Gerritsen (2003 pp 3-15 3-16 6-2) and DEQ (2008)
q
Appendix C
LABORATORY BENCH SHEETS
Shenandoah Universityrsquos Environmental Studies Department developed the Benthic Macroinvertebrate Laboratory Bench Sheet and VSCI Worksheet to record BMI specimen identifications and their VSCI classifications for each sample
This form is based in part on bench sheets developed by the EPA and Virginia DEQ for recording specimen identifications Additional columns on Shenandoah Universityrsquos forms for relative numbers and VSCI classifications make the calculation and verification of VSCI scores easier
Note the column for taxonomistrsquos initials Specimens were identified by students in Shenandoah Universityrsquos research group and then verified or where necessary changed by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality This external review helps assure the validity of the study Originals of these bench sheets with handwritten taxonomistsrsquo initials will be kept on file at Shenandoah Universityrsquos Environmental Studies Department for five years after the completion of this study
r
s
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-1 STATION NAME Mouth LAT N 39deg 12749rsquo (upstream) LONG W 078deg 04209rsquo (upstream) LAT N 39deg 12729rsquo (downstream) LONG W 078deg 04148rsquo (downstream) FORM COMPILED BY Jeremy Tovar W S Bousquet RIVER BASIN Potomac River INVESTIGATORS Marie Dahl Brandon Millholand J W Pangle Sean Robertson Jeremy Tovar Woodward Bousquet Ben Bousquet
Collection date 21 May 2008 Sheet completion date 12 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Hirudinea 3 33 JT BVW PR 8 SP Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Coenagrionidae 2 22 BVW PR 9 CR Ephemeroptera (mayflies) Caenidae 1 11 JT BVW CG 6 SP Ephemerllidae 4 44 JT BVW CG 2 CR Heptageniidae 5 55 JT BVW SC 3 CG Isonychiidae 1 11 BVW CF 3 CG Plecoptera (stoneflies) Perlidae 14 154 JT BVW PR 2 CR Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Corydalidae 1 11 JT BVW PR 5 CR Coleoptera (beetles) Elmidae 24 264 I JT BVW SC 4 CG Dytiscidae 4 44 JT BVW PR 6 GN Psephenidae 3 33 JT BVW SC 4 CG Diptera (true flies) Tipulidae 3 33 JT BVW SH 4 BU Chironomidae 26 286 JT BVW CG 6 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
t
Other Total Individuals 91 1001 Total Taxa 13
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
u
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-1 STATION NAME Mouth LAT N 39deg 12749rsquo (upstream) LONG W 078deg 04209rsquo (upstream) LAT N 39deg 12729rsquo (downstream) LONG W 078deg 04148rsquo (downstream) FORM COMPILED BY Jeremy Tovar W S Bousquet RIVER BASIN Potomac River INVESTIGATORS Brandon Millholand JW Pangle Jeremy Tovar Heather Kepple
Collection date 10 October 2008 Sheet completion date 12 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Oligochaeta 3 35 JT BVW CG 8 BU Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Coenagrionidae 2 23 JT BVW PR 9 CR Ephemeroptera (mayflies) Heptageniidae 12 140 JT BVW SC 3 CG Isonychiidae 2 23 BVW CF 3 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Hydropsychidae 16 186 JT BVW CF 6 CG Philopotamidae 31 360 JT BVW CF 2 CG Hemiptera (true bugs) (Veliidae) (5) - JT BVW ( ) Megaloptera (dobsonflies etc) Corydalidae 10 116 JT BVW PR 5 CR Coleoptera (beetles) Dytiscidae 1 12 JT BVW PR 6 GN Psephenidae 7 81 JT BVW SC 4 CG Diptera (true flies) Tipulidae 2 23 BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
v
Other Total Individuals 86
(91) 999
Total Taxa 10 (11)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009 Veliidae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
w
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-2 STATION NAME Package Plant LAT N 39deg 12958rsquo (upstream) LONG W 078deg 04900 (upstream) LAT N 39deg 12958rsquo (downstream) LONG W 078deg 04822 (downstream) FORM COMPILED BY JW Pangle W Bousquet RIVER BASIN Potomac River INVESTIGATORS JW Pangle S Robertson J Tovar M Dahl B Millholland W Bousquet
Collection date 29 May 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Plecoptera (stoneflies) Perlidae 11 136 I JP BVW PR 1 CR Trichoptera (caddisflies) Hydropsychidae 10 123 I JP BVW CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 14 173 I JP BVW SC 4 CG Diptera (true flies) Chironomidae 32 395 I JP BVW CG 6 BU Simuliidae 14 173 I JP BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
x
Other Total Individuals 81 1000 Total Taxa 5
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-2 STATION NAME Package Plant LAT N 39deg 12958rsquo (upstream) LONG W 078deg 04900 (upstream) LAT N 39deg 12958rsquo (downstream) LONG W 078deg 04822 (downstream) FORM COMPILED BY JW Pangle W Bousquet RIVER BASIN Potomac River INVESTIGATORS JW Pangle Brandon Millholland Jeremy Tovar Heather Kepple
Collection date 10 October 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Heptageniidae 40 392 I WBJP SC 3 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Hydropsychidae 7 69 I JP WB BVW CF 6 CG Philopotamidae 28 275 I JP WB BVW CF 2 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Corydalidae 6 59 I JP WB BVW PR 5 CR Coleoptera (beetles) Psephenidae 7 69 I JP WB BVW SC 4 CG Elmidae 8 78 I JP WB BVW SC 4 CG Diptera (true flies) Tipulidae 6 59 I JP WB BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
y
z
Other Total Individuals 102 1001 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-3 STATION NAME Rt 664 Bridge LAT N 39deg 12934rsquo (upstream) LONG W 078deg 05019rsquo (upstream) LAT N 39deg 12951rsquo (downstream) LONG W 078deg 04958rsquo (downstream) FORM COMPILED BY S Robertson W Bousquet RIVER BASIN Potomac River INVESTIGATORS SRobertson BMillholland WBousquet MDahl JPangle
Collection date 28 May 2008 Sheet completion date 11 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Leptophlebiidae 1 15 I SGR BVW CG 2 CR Baetidae 1 15 I BVW CG 5 CG Plecoptera (stoneflies) Perlidae 3 46 I SGR BVW PR 1 CR Nemouridae 1 15 I BVW SH 3 CR Trichoptera (caddisflies) Hydropsychidae 5 77 I SGR BVW CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 27 415 I SGR BVW SC 4 CG Psephenidae 2 31 A SGR BVW SC 4 CG Diptera (true flies) Simuliidae 5 77 I SGR BVW CF 6 CG Chironomidae 20 308 I SGR BVW CG 6 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
aa
bb
Other Total Individuals 65 999 Total Taxa 9
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-3 STATION NAME Rt 664 Bridge LAT N 39deg 12934rsquo (upstream) LONG W 078deg 05019rsquo (upstream) LAT N 39deg 12951rsquo (downstream) LONG W 078deg 04958rsquo (downstream) FORM COMPILED BY S Robertson RIVER BASIN Potomac River INVESTIGATORS S Robertson M Dahl A Fisher H Kepple
Collection date 10 Oct 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Heptageniidae 2 20 I SGR SC 3 CG Isonychiidae 2 20 I BVW CF 2 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Philopotamidae 36 367 I SGR CF 2 CG Hydropsychidae 47 480 I SGR CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 2 20 A SGR SC 4 CG Psephenidae 8 82 A SGR SC 4 CG Diptera (true flies) Tabanidae 1 10 I SGR PR 7 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
cc
dd
Other Total Individuals 98 999 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-4 STATION NAME Unnamed Tributary LAT (upstream) N 39deg 13035rsquo LONG (upstream) W 078deg 05129rsquo LAT (downstream) N 39deg 13000rsquo LONG (downstream) W 078deg 5086rsquo FORM COMPILED BY A Fisher M Dahl rev W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson A Fisher H Kepple
Collection date 31 May 2008 Sheet completion date rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Flatworms (Turbellaria) Worms (Oligochaeta) Naididae 1 14 - BVW CG 8 BU Leeches (Hirudinea) Snails (Gastropoda) Clams (Bivalvia) Isopods (Isopoda) Asellidae 3 42 A AF BVW CG 8 SP Scuds (Amphipoda) Crayfish Shrimp (Decapoda) Water Mites (Hydracarina) Dragonflies (Odonata Anisoptera) Damselflies (Odonata Zygoptera) Mayflies (Ephemeroptera) Stoneflies (Plecoptera) Perlidae 2 28 I AF BVW PR 1 CR Caddisflies (Trichoptera) True Bugs (Hemiptera) Corixidae 1 14 I AF BVW MP 9 DV Dobsonflies (Megaloptera) Beetles (Coleoptera) Dytiscidae 1 14 I AF BVW PR 6 GN True Flies (Diptera) Chironomidae 61 847 I AF BVW CG 6 BU Simuliidae 3 42 I AF BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
ee
ff
Other Total Individuals 72 1001 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-4 STATION NAME Unnamed Tributary LAT (upstream) N 39deg 13035rsquo LONG (upstream) W 078deg 05129rsquo LAT (downstream) N 39deg 13000rsquo LONG (downstream) W 078deg 5086rsquo FORM COMPILED BY Andrew G M Fisher W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson A Fisher H Kepple
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Flatworms (Turbellaria) Worms (Oligochaeta) Lumbriculidae 2 32 A AF BVW CG 8 BU Leeches (Hirudinea) Snails (Gastropoda) Lymnaeidae 1 16 A BVW CG 9 GN Clams (Bivalvia) Isopods (Isopoda) Scuds (Amphipoda) Crayfish Shrimp (Decapoda) Cambaridae 1 16 A AF BVW GN 5 GN Water Mites (Hydracarina) Dragonflies (Odonata Anisoptera) Damselflies (Odonata Zygoptera) Calopterygidae 4 63 I BVW PR 7 CL Mayflies (Ephemeroptera) Tricorythidae 1 16 I BVW CG 5 SP Stoneflies (Plecoptera) Caddisflies (Trichoptera) Phryganeidae 4 63 I AF BVW SH 4 CR Hydropsychidae 6 95 I AF BVW CF 6 CG I True Bugs (Hemiptera) Corixidae 8 127 I AF BVW MP 9 DV (Veliidae ) (7) - I AF BVW ( ) Dobsonflies (Megaloptera) Sialidae 3 48 I AF BVW PR 7 SP Corydalidae 2 32 I AF BVW PR 5 CR Beetles (Coleoptera) Haliplidae 1 16 A AF BVW MP 8 GN Dysticidae 7 111 I AF BVW PR 6 GN Hydrophilidae 1 16 A AF BVW PR 8 GN Elmidae 3 48 I BVW SC 4 CG True Flies (Diptera) Chironomidae 8 127 I AF BVW CG 6 BU Simuliidae 7 111 I AF BVW CF 6 CG Tipulidae 4 63 I AF BVW SH 4 GU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
gg
hh
Other Total Individuals 63
(70) 1000
Total Taxa 17 (18)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Veliidae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-5 STATION NAME Lick Run LAT N 39deg 12752rsquo (upstream) LONG W 078deg 05685rsquo (upstream) LAT N 39deg 12784rsquo (downstream) LONG W 078deg 05631rsquo (downstream) FORM COMPILED BY Brandon Millholland W Bousquet RIVER BASIN Potomac River INVESTIGATORS J Pangle S Robertson W Bousquet JTovar M Dahl
Collection date 29 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Gomphidae 1 15 I BVW PR 3 BU Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Leptophlebiidae 11 167 A LBM BVW CG 2 CR Plecoptera (stoneflies) Perlidae 10 152 A LBM BVW PR 1 CR Perlodiae 1 15 A LBM BVW PR 2 CR Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 5 76 A LBM BVW SC 4 CG Elmidae 2 30 I LBM BVW SC 4 CG Diptera (true flies) (Culicidae) (3) - P LMB BVW ( ) Chironomidae 23 348 I LBM BVW CG 6 BU Simuliidae 13 197 I LBM BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
ii
jj
Other Total Individuals 66
(69) 1000
Total Taxa 7 (8)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Culicidae (Diptera) pupae not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl J Pangle S Robertson W Bousquet B Millholland J Tovar H Kepple
Collection date 30 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 1 12 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Asellidae 114 965 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Plecoptera (stoneflies) Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 2 23 A MRD BVW SC 4 CG Diptera (true flies)
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
kk
ll
Other Total Individuals 117 1000 Total Taxa 3
Notes Subsample taken 29 October 2009 All taxonomic determinations by MRD were confirmed by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson K McCann A Fisher H Kepple A Barrett
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 2 17 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Sphaeriidae 2 17 I MRD BVW CF 8 BU Isopoda (isopods) Asellidae 99 853 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Calopterygidae 2 17 A MRD BVW PR 7 CL Ephemeroptera (mayflies) Leptophlebiidae 2 17 I MRD BVW CG 2 CR Heptageniidae 4 34 A MRD BVW SC 3 CG Plecoptera (stoneflies) Perlidae 1 09 A MRD BVW PR 1 CR Trichoptera (caddisflies) Hemiptera (true bugs) (Gerridae) (1) - A MRD BVW ( ) Corixidae 1 09 A MRD BVW PR 5 CR Megaloptera (dobsonflies etc) Coleoptera (beetles) Dryopidae 1 09 A MRD BVW SC 5 CG Diptera (true flies) Tipulidae 2 17 I BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
mm
nn
Other Total Individuals 116
(117) 999
Total Taxa 10 (11)
Notes Subsample taken 6 November 2008 Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009 Gerridae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
- Disclaimer ii
- Title Page iii
- Acknowledgements v
- Table of Contents vii
- SECTION 2 RESULTS DATA TABLES 7
- OVERVIEW AND FINDINGS
- Introduction
- RESULTS DATA TABLES
- REFERENCES
- FIELD DATA SHEETS
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- SubstrateType
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- WATER QUALITY ANALYSIS
- THROUGH THE VIRGINIA
- STREAM CONDITION INDEX (VSCI)
- LABORATORY BENCH SHEETS
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
Biological Monitoring in Hiatt and Lick Runs Opequon Creek Watershed Frederick County Virginia
2008 Baseline Data and a Monitoring Framework
An Investigation by
Marie R Dahl Woodward S Bousquet L Brandon Millholland J W Pangle Sean G Robertson Jeremy D Tovar
Jared B Davis and Andrew G M Fisher
Submitted by the Student Authors in Partial Fulfillment of the Requirements for
Environmental Measurement Methods (ES 421) Research Project in Environmental Studies (ES 492) andor
Research Project in Biology (BIO 492)
Technical Report No 3 Environmental Studies Department College of Arts and Sciences
Shenandoah University 1460 University Drive Winchester Virginia 22601
May 2009 Online Edition July 2010
iii
This report may be cited as follows Dahl Marie R Woodward S Bousquet L Brandon Millholland J W Pangle Sean G Robertson Jeremy D Tovar Jared B Davis and Andrew G M Fisher 2009 (online edition 2010) Biological Monitoring in Hiatt and Lick Runs Opequon Creek Watershed Frederick County Virginia 2008 Baseline Data and a Monitoring Framework Winchester Virginia Environmental Studies Program Shenandoah University
iv
ACKNOWLEDGEMENTS
Many individuals and organizations provided time support and expertise for this research
study Although we cannot list everyone by name we would like to express our sincere gratitude to all who made the project possible including Jim Lawrence ndash Coordinator Opequon Targeted Watershed Project Property owners along Hiatt Run and Lick Run
Mike Hoffman Leslie Johns The McCann-Slaughter Family Ervin and Barbara Simons Tonie Wallace-Aitken and Greg Aitken Historic Jordan Springs Event and Cultural Centre
Billy van Wart ndash Regional Aquatic Biologist Virginia Department of Environmental Quality Heather Kepple ndash Technician Opequon Targeted Watershed Project Field Assistance Andrew Barrett
Ben Bousquet Katherine McCann
Financial Support
Opequon Creek Targeted Watershed Grant Dr and Mrs Charles Leeper An anonymous family foundation Shenandoah University
v
vi
TABLE OF CONTENTS page
Disclaimer ii Title Page iii Acknowledgements v Table of Contents vii SECTION 1 OVERVIEW AND FINDINGS 1 Introduction 1 Methods and Analysis 3 Findings 3 Future Research 5 SECTION 2 RESULTS DATA TABLES 7 Site HLR-1 Mouth 7 Site HLR-2 Package Plant 8 Site HLR-3 Route 664 Bridge 9 Site HLR-4 Unnamed Tributary 10 Site HLR-5 Lick Run 11 Site HLR-6 McCann Farm 12 REFERENCES 13 APPENDICES 15 Appendix A Field Data Sheets Appendix B Water Quality Analysis through the Virginia Stream Condition Index (VSCI) Appendix C Laboratory Bench Sheets Note Photographs of the six sampling sites appear on a CD provided separately to the grant administrator
vii
viii
Section 1
OVERVIEW AND FINDINGS
Introduction
This report presents baseline water quality data for Hiatt Run and Lick Run -- termed the Hiatt-Lick Run subwatershed in this study Located in Frederick County Virginia Hiatt and Lick Runs are tributaries of Opequon Creek The Opequon in turn empties into the Potomac River and then the Chesapeake Bay The investigation was conducted by a Shenandoah University research team in 2008 Researchers used benthic macroinvertebrates (BMIs) as biological indicators of water quality This report presents an overview of the study followed by summary tables and a list of references The Appendix contains data sheets and a description of the data analysis These data will form part of the baseline water quality assessment that is being undertaken through the Opequon Creek Targeted Watershed Grant The grant administered by Jim Lawrence reflects a broad-based partnership that includes Virginia Tech Shenandoah University the Frederick-Winchester Service Authority The Opequon Watershed (TOW) citizens organization as well as federal state and local governments community groups and business interests Grant partners will use best management practices (BMPs) to reduce nutrient runoff and address other pollution problems in the Opequon Creek watershed Since 1996 Opequon Creek has appeared on the impaired waters list prepared by the Virginia Department of Environmental Quality (DEQ) The Hiatt-Lick Run subwatershed was selected for this study because it represents changes that are typical of the Opequon Creek watershed These changes include new commercial and residential developments as well as the implementation of water quality improvement practices such as tree planting in riparian zones fences to prevent livestock from entering streams and replacing outdated sewage treatment facilities such as a package plant on lower Hiatt Run
Seven students from Shenandoah Universityrsquos Environmental Studies Department conducted this study from May through December 2008 under the direction of Woodward Bousquet Professor of Environmental Studies and Biology The research team followed procedures described in the Rapid Bioassessment Protocols (RBPs) of the US Environmental Protection Agency (Barbour et al 1999) and the Virginia Stream Condition Index (VSCI) The VSCI provides an evaluation of water quality based on a streamrsquos benthic macroinvertebrate community (Burton and Gerritsen 2003 DEQ 2006b and 2008)
In consultation with Billy van Wart of the DEQ and grant administrator Jim Lawrence the Shenandoah University researchers selected six representative sampling sites in the Hiatt-Lick Run subwatershed Each site was given a name and an HLR (for Hiatt-Lick Run) code Figure 1 maps their positions while their characteristics and coordinates appear in the field data sheets (see Appendix A) These sites were established according to EPA and DEQ guidelines that generally call for a 100-meter-long stream reach with at least three riffle zones a width sufficient to accommodate a 1-meter seine and a substrate composed of least 35 cobbles
Specific locations of the sampling sites on Hiatt and Lick Runs were selected to reflect different watershed positions and changing land uses See Figure 1 Further information about each sampling site appears in the field data sheets (Appendix A)
1
Figure 1 Sampling Sites in the Hiatt-Lick Run Subwatershed of Opequon Creek Frederick County Virginia Scale 1 centimeter = 500 meters
HLR-1 (Mouth) measures water quality at the lowest point of the subwatershed as Hiatt Run flows into Opequon Creek Forests cover the immediate riparian zones on both sides of Hiatt Run This site will be used in future years by the DEQ for BMI monitoring HLR-2 (Package Plant) is situated on Hiatt Run just below the outflow pipe of an outdated package plant that formerly treated sewage from the Jordan Springs area During the study period the package plant was taken off-line and a pumping station was constructed The Frederick-Winchester Service Authority now treats this sewage at its Opequon Water Reclamation Facility HLR-3 (Route 664 Bridge) was chosen because it is located upstream from the package plant but downstream of the confluence of Hiatt Run and Lick Run HLR-4 (Unnamed Tributary) is located downstream of the Snowden Bridge residential development which has been under construction The area immediately upstream of this sampling site was being graded by heavy equipment during the May 2008 sampling time HLR-5 (Lick Run) the researchers learned dries up in late summer It had a modest but constant flow during when the May 2008 sample was taken HLR-6 (McCann Farm) represents the headwater areas of the Hiatt-Lick Run subwatershed of Opequon Creek The McCanns who graze cattle in their pastures that surround Hiatt Run here have been working with the Potomac Conservancy to implement Best Management Practices
2
(BMPs) to improve water quality These practices include fencing cattle out of the stream and spring runs and planting more trees in the riparian zone
Methods and Analysis
The research team established a sampling plan and then sampled according to methods the DEQ developed for the VSCI Using a 1 meter x 1 meter seine samples from 2m2 of substrate in riffle zones were to be collected twice at each site once in May 2008 and once in October 2008 At five of the six sites stream characteristics permitted the standard single-habitat sampling procedure At the McCann Farm (Site HLR-6) researchers needed to use multi-habitat sampling See Barbour et al (1999 pp 7-3 ff) for further descriptions of these two methods
All six sites were sampled in May 2008 Low-water conditions in October 2008 prevented the Shenandoah University group from collecting BMIs at Lick Run (HLR-5) that month but the other five sites were sampled successfully As a result the research team obtained eleven samples for analysis in this study Samples were preserved in ethanol for subsequent identification
DEQ procedures require subsampling when the number of BMIs in a given sample exceeds 100 All eleven samples in this study met that criterion Consequently researchers used a Caton tray to produce random subsamples of 90 to 110 specimens from each sample During the analysis that followed some specimens in the subsamples were rejected either because they turned out to be terrestrial species or because they were in the wrong life stage (eg pupae) In most cases the researchers identified specimens to the family level Primary taxonomic references were A Guide to Common Freshwater Invertebrates of North America (Voshell 2002) Fresh-Water Invertebrates of the United States (Pennak 1953) and An Introduction to the Aquatic Insects of North America (Merritt and Cummins 1996) DEQ Regional Aquatic Biologist Billy van Wart reviewed verified and where necessary corrected the identifications
The investigators then used the Virginia Stream Condition Index (VSCI) to produce water quality scores based on the BMIs collected A VSCI score is based on eight different measurement formulas called metrics Each metric is a numeric measure of a biological communityrsquos species composition diversity ecological niches or tolerance to pollution When combined into a single multimetric VSCI score the response of the benthic community to water conditions can be evaluated For further information and worksheets see Appendices B and C respectively
Findings
Table 1 presents the VSCI scores for the eleven samples collected in the Hiatt-Lick Run subwatershed in 2008 Since a sample year consists of two samples the mean scores for each site for the entire year are also included As the table shows
bull None of the 11 samples was rated excellent bull Only 1 of the 11 samples was rated as good bull Only 2 of the 11 samples were rated fair (ie in the ldquogrey zonerdquo between impaired and
unimpaired) bull The remaining 8 samples were in either moderately stressed or severely stressed
condition
3
Site Name and Code Sampling
Date of
TaxaVCSI Score
Water Quality Rating
Mouth (HLR-1) 21 May 2008 13 571 Fair (Gray Zone)
Mouth (HLR-1) 10 Oct 2008 10 608 Fair (Gray Zone)
site average for 2008 590 Fair (Gray Zone)
Package Plant (HLR-2) 29 May 2008 5 370 Severely Stressed
Package Plant (HLR-2) 10 Oct 2008 7 674 Good
site average for 2008 522 Moderately Stressed
Route 664 Bridge (HLR-3) 28 May 2008 9 485 Moderately Stressed
Route 664 Bridge (HLR-3) 10 Oct 2008 7 469 Moderately Stressed
site average for 2008 477 Moderately Stressed
Unnamed Tributary (HLR-4) 31 May 2008 7 173 Severely Stressed
Unnamed Tributary (HLR-4) 10 Oct 2008 17 476 Moderately Stressed
site average for 2008 325 Severely Stressed
Upper Lick Run (HLR-5) 29 May 2008 7 455 Moderately Stressed
site average for 2008 455 Moderately Stressed
McCann Farm (HLR-6) 30 May 2008 3 190 Severely Stressed
McCann Farm (HLR-6) 10 Oct 2008 10 316 Severely Stressed
site average for 2008 253 Severely Stressed
Summary 6 sites 11 samples
36 BMI taxa
VSCI = Virginia Stream Condition Index see Burton and Gerritsen (2003) and Virginia Department of Environmental Quality (2006b and 2008) Table 1 Summary Table of Virginia Stream Condition Index (VSCI) Scores and Water Quality Ratings for Sites in the Hiatt-Lick Run Subwatershed of Opequon Creek Frederick County Virginia 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
4
Improvements in water quality ratings at three sites between May and October suggest that BMPs and other changes may already be having some positive effects on water quality At the Package Plant (Site HLR-2) the VSCI score rose dramatically from 370 (severely stressed) to 674 (good) The small-scale sewage treatment plant as noted above was taken off-line during this period Similarly water quality at the Unnamed Tributary (HLR-4) increased from 173 (severely stressed) to 476 (moderately stressed) Adjacent construction was completed over the summer and grass was planted on the property Management practices on the McCann Farm (HLR-6) may be responsible for improving conditions for aquatic life in Hiatt Runrsquos headwaters Even though the water quality was still rated as severely stressed in October the VSCI score rose from 190 to 316 at this sampling site Further studies are needed to determine whether the changes noted in 2008 persist into the future In 2003 the DEQ began a Total Maximum Daily Load (TMDL) process for Opequon Creek as required by the Water Quality Act of 1987 The TMDL approach establishes a ldquopollution budgetrdquo for each impaired stream for each type of impairment and for each responsible pollutant In conjunction with the DEQ and the Virginia Department of Conservation and Recreation (DCR) Virginia Polytechnic Institutersquos Department of Biosystems Engineering developed a TMDL implementation plan (Opequon Creek IP Steering Committee et al 2006) Monitoring the BMI communities of Opequon Creek and its tributaries is essential to evaluating the progress of the proposed water quality improvement projects
Future Research The relatively small Hiatt-Lick Run subwatershed provides several case studies that can help assess the effectiveness of various water quality management practices not only in Opequon Creek but also in other watersheds in the Chesapeake Bay region and beyond In supporting that goal this study makes two principal contributions 1 It provides baseline data to measure the improvement or degradation of the Hiatt-Lick Run section of the Opequon Creek watershed and 2 It establishes a monitoring framework for the long-term biological monitoring of water quality and the evaluation of best management practices (BMPs) This framework consists of
bull Sampling Sites ndash six stream reaches in the Hiatt-Lick Run subwatershed selected as sampling sites according to EPA and DEQ specifications
bull Data Collection Protocols ndash based on the EPArsquos Rapid Bioassessment Protocols (RBPs)
(Barbour et al 1999) and the DEQrsquos Virginia Stream Index (VSCI) (Burton and Gerritsen 2003 DEQ 2006b and 2008) and
bull Analytical Procedures ndash contained in the Virginia Stream Condition Index (VSCI see
Burton and Gerritsen 2003 DEQ 2006b pp 8 and 33 and DEQ 2008 pp 29-34)
5
6
Section 2
RESULTS DATA TABLES
Site HLR-1 Mouth
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 13 189 6872 EPT taxa 5 117 4273 Ephemeroptera 121 477 2544 Plec +Tric less Hydropsych 154 562 2745 Scrapers 352 467 7536 Chironomidae 286 16 7267 Top 2 Dominant 550 328 6708 Hilsenhoff Biotic Index (HBI) 45 29 775VSCI Score 571
According to the DEQrsquos water quality standards aquatic life at this site is fair (gray zone) See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 2 VSCI Scores for Mouth (HLR-1) Site in Hiatt-Lick Run Sampled on 21 May 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 10 189 5292 EPT taxa 4 117 3423 Ephemeroptera 163 477 3424 Plec +Tric less Hydropsych 360 562 6415 Scrapers 221 467 4736 Chironomidae 00 16 10007 Top 2 Dominant 546 328 6768 Hilsenhoff Biotic Index (HBI) 39 29 859VSCI Score 608
According to the DEQrsquos water quality standards aquatic life at this site is fair (gray zone) See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 3 VSCI Scores for Mouth (HLR-1) Site in Hiatt-Lick Run Sampled 10 October 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
7
Site HLR-2 Package Plant
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 5 189 2642 EPT taxa 2 117 1713 Ephemeroptera 00 477 004 Plec +Tric less Hydropsych 110 562 1965 Scrapers 173 467 3706 Chironomidae 395 16 6157 Top 2 Dominant 568 328 6438 Hilsenhoff Biotic Index (HBI) 50 29 704VSCI Score 370
According to the DEQrsquos water quality standards aquatic life at this site is severely stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 4 VSCI Scores for Package Plant (HLR-2) Site in Hiatt-Lick Run Sampled 29 May 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 7 189 3702 EPT taxa 34 117 2563 Ephemeroptera 392 477 8214 Plec +Tric less Hydropsych 275 562 4895 Scrapers 539 467 10006 Chironomidae 00 16 10007 Top 2 Dominant 667 328 4968 Hilsenhoff Biotic Index (HBI) 32 29 958VSCI Score 674
According to the DEQrsquos water quality standards aquatic life at this site is good See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 5 VSCI Scores for Package Plant (HLR-2) Site in Hiatt-Lick Run Sampled 10 October 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
8
Site HLR-3 Route 664 Bridge
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 9 189 4762 EPT taxa 5 117 4273 Ephemeroptera 30 477 634 Plec +Tric less Hydropsych 61 562 1095 Scrapers 446 467 9556 Chironomidae 308 16 7037 Top 2 Dominant 723 328 4128 Hilsenhoff Biotic Index (HBI) 48 29 732VSCI Score 485
According to the DEQrsquos water quality standards aquatic life at this site is moderately stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 6 VSCI Scores for Route 664 Bridge (HLR-3) Site in Hiatt-Lick Run Sampled 28 May 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 7 189 3702 EPT taxa 4 117 3423 Ephemeroptera 40 477 844 Plec +Tric less Hydropsych 367 562 6535 Scrapers 122 467 2616 Chironomidae 00 16 10007 Top 2 Dominant 847 328 2288 Hilsenhoff Biotic Index (HBI) 42 29 817VSCI Score 469
According to the DEQrsquos water quality standards aquatic life at this site is moderately stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 7 VSCI Scores for Route 664 Bridge (HLR-3) Site in Hiatt-Lick Run Sampled 10 October 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
9
Site HLR-4 Unnamed Tributary
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 7 189 3702 EPT taxa 1 117 853 Ephemeroptera 00 477 004 Plec +Tric less Hydropsych 28 562 495 Scrapers 00 467 006 Chironomidae 847 16 1557 Top 2 Dominant 889 328 1658 Hilsenhoff Biotic Index (HBI) 60 29 563VSCI Score 173
According to the DEQrsquos water quality standards aquatic life at this site is severely stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 8 VSCI Scores for Unnamed Tributary (HLR-4) Site in Hiatt-Lick Run Sampled 31 May 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 17 189 8992 EPT taxa 3 117 2563 Ephemeroptera 16 477 344 Plec +Tric less Hydropsych 63 562 1125 Scrapers 48 467 1036 Chironomidae 127 16 8877 Top 2 Dominant 254 328 10008 Hilsenhoff Biotic Index (HBI) 63 29 521VSCI Score 476
According to the DEQrsquos water quality standards aquatic life at this site is moderately stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 9 VSCI Scores for Unnamed Tributary (HLR-4) Site in Hiatt-Lick Run Sampled 10 October 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
10
Site HLR-5 Lick Run
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 7 189 3702 EPT taxa 3 117 2563 Ephemeroptera 167 477 3504 Plec +Tric less Hydropsych 167 562 2975 Scrapers 106 467 2276 Chironomidae 348 16 6637 Top 2 Dominant 545 328 6778 Hilsenhoff Biotic Index (HBI) 43 29 803VSCI Score 455
According to the DEQrsquos water quality standards aquatic life at this site is moderately stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 10 VSCI Scores for Lick Run (HLR-5) Site in Hiatt-Lick Run Sampled 29 May 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
Note
Water conditions at the Lick Run (HLR-5) site were too low in October 2008 to permit a sample to be taken
11
Site HLR-6 McCann Farm
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 3 189 1592 EPT taxa 0 117 003 Ephemeroptera 00 477 004 Plec +Tric less Hydropsych 00 562 005 Scrapers 23 467 496 Chironomidae 00 16 10007 Top 2 Dominant 988 328 188 Hilsenhoff Biotic Index (HBI) 79 29 296VSCI Score 190
According to the DEQrsquos water quality standards aquatic life at this site is severely stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 11 VSCI Scores for McCann Farm (HLR-6) Site in Hiatt-Lick Run Sampled 30 May 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 10 189 5292 EPT taxa 3 117 2563 Ephemeroptera 51 477 1074 Plec +Tric less Hydropsych 09 562 165 Scrapers 43 467 926 Chironomidae 00 16 10007 Top 2 Dominant 887 328 1688 Hilsenhoff Biotic Index (HBI) 75 29 352VSCI Score 315
According to the DEQrsquos water quality standards aquatic life at this site is severely stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 12 VSCI Scores for McCann Farm (HLR-6) Site in Hiatt-Lick Run Sampled 10 October 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
12
REFERENCES
Barbour Michael T Jeroen Gerritsen Blaine D Snyder and James B Stribling 1999 Rapid bioassessment protocols for use in streams and wadeable rivers periphyton benthic macroinvertebrates and fish Second edition EPA 841-B-99-002 Washington DC US Environmental Protection Agency Office of Water Available online at httpwwwepagovOWOWmonitoringbioasspshtml Burton June and Jeroen Gerritsen 2003 A stream condition index for Virginia non-coastal streams Prepared for the US EPA Office of Science and Technology US EPA Region 3 Environmental Services Division and Virginia Department of Environmental Quality Owings Mills Maryland Tetra Tech Inc Available online at httpwwwdeqvirginiagovwatermonitoringpdf vastrmconpdf Hessler Rachel A Andrea R Martin Heidi D Ruggeri William B Seymour Jr and Woodward S Bousquet 2006 Water quality assessment using benthic macroinvertebrates A study and compilation Winchester Virginia Environmental Studies Program Shenandoah University Occasional publication no 10 Igoe Robert 2006 DEQ identifies nine impaired streams in Clarke Frederick The Winchester Star 15 July B1 Jackson Charlie 2005 Survey targets Opequon cleanup The Winchester Star 19 August B1 Johnston Elizabeth A and Woodward S Bousquet 2003 What happens when it rains A study of water quality in Abrams Creek and Town Run Winchester and Frederick County Virginia Winchester Virginia Environmental Studies Program Shenandoah University Occasional publication no7 Merritt Richard W and Kenneth W Cummins 1996 An introduction to the aquatic insects of North America Dubuque Iowa KendallHunt Publishing Company Opequon Creek IP Steering Committee Virginia Tech Department of Biological Systems Engineering and Center for TMDL and Watershed Studies University of Virginia Institute for Environmental Negotiation and West Virginia University Division of Resource Management 2006 Opequon Creek watershed TMDL implementation plan Blacksburg Virginia Virginia Tech Department of Biological Systems Engineering Available online at httpwwwtmdlbsevteduoutreachC82 Pennak Robert W 1953 Fresh-water invertebrates of the United States New York New York The Ronald Press Company Rosenburg David M and Vincent H Resh 1993 Freshwater biomonitoring and benthic macroinvertebrates New York New York Chapman amp Hall Shenandoah University Public Relations Office 2002 Data collected by SU and its partners helps identify impaired water Winchester Virginia Available online at httpwwwsuedutemp_ newscfmurlnum=32 Thorp James H and Alan P Covich 1991 Ecology and classification of North American freshwater invertebrates San Diego California Academic Press Inc
13
US Environmental Protection Agency 1997 Volunteer stream monitoring a methods manual EPA 841-B-97-003 Washington DC US Environmental Protection Agency Office of Water Van Meter Val 2003 Opequon Creek pollution limits to be established The Winchester Star 4 April B1 Virginia Department of Environmental Quality 2006a Virginia 305(b)303(d) water quality integrated report to Congress and the EPA Administrator for the period January 1 2000 to December 31 2004 Richmond Virginia Virginia Department of Environmental Quality Available online at httpwwwdeqvirginiagovwqair2006html __________ 2006b Using probabilistic data to validate the non-coastal Virginia Stream Condition Index (draft) VDEQ technical bulletin WQA2006-001 Virginia Department of Environmental Quality Available online at httpwwwloudounwatershedorgstream_assessmentreference_documents VA_DEQ_SCI_Validationpdf __________ 2008 Biological monitoring program quality assurance project plan for wadeable streams and rivers Richmond Virginia Virginia Department of Environmental Quality Update available online at httpwwwdeqstatevausbiomonitoringdocumentsBioMonQAPP_13Aug2008pdf Virginia Tech Aquatic Entomology Laboratory 2005 Taxa dictionary [Excel file] Blacksburg Virginia Virginia Tech Department of Biological Systems Engineering 2003a Opequon watershed TMDLs for benthic impairments Abrams Creek and Lower Opequon Creek Frederick and Clarke Counties Virginia Prepared for the Virginia Department of Environmental Quality and Virginia Department of Conservation and Recreation Blacksburg Virginia Available online at httpwwwdeqstate vaustmdlapptmdlsshenrvrabropebcpdf Voshell J Reese Jr 2002 A guide to common freshwater invertebrates of North America Blacksburg Virginia The McDonald amp Woodward Publishing Company
14
APPENDICES
15
16
a
Appendix A
FIELD DATA SHEETS
This appendix presents a Site Characterization Field Data Sheet for each of the six sampling reaches established on Hiatt Run and Lick Run for this study They were filled out during the sampling runs made in May 2008
These forms were adapted by Shenandoah University from forms used by the EPA and Virginia DEQ for recording characteristics of sampling sites
b
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Mouth STATION _HLR-1_____ STREAM CLASS LAT N 39 12749 (upstream) LONG W 078 04209(upstream) LAT N 39 12729 (downstream) LONG W 078 04148 (downstream) FORM COMPILED BY Jeremy Tovar RIVER BASIN Potomac River INVESTIGATORS JPangle B Millholland MDahl SRobertson W Bousquet JTovar BBousquet
DATE May 21 2008_________ TIME_250 PM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature __24___degC showers (intermittent) Comments _______________________
___ cloud cover _0_ ______________________________
X clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x ( Significant springs andor seeps) x (Valley ) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS
c
d
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence x FieldPasture Industrial Obvious sources Agricultural Other ____________ Stream scour steep slope erosion x Residential Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left Field and young thin deciduous forest 100 Bank=Grass cobble and shrub Right 90 maturing deciduous forest 45 shale slope Bank=Bedrock soil and herbs Indicate the dominant vegetation type(s) and record the dominant species
x Trees Shrubs x (Grasses) Forbs Dominant species Oak species and mix deciduous
INSTREAM FEATURES
Measured reach length ___100__m Canopy Cover Estimated stream width _35__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area __350_m2
Estimated stream depth _008_m Proportion of Reach Represented by Stream High water mark _15_m Morphology Types Surface velocity (at thalweg) _05__msec x Riffle__75___ x Run__20___ x Pool__5___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___00__ m2 Density of LWD _-_ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __15_____ x Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present Algae
WATER QUALITY Temperature __19___degC Water Odors x NormalNone Sewage Specific Conductance - Petroleum Chemical Fishy Other___________________ Dissolved Oxygen - Water Surface Oils Slick Sheen Globs Flecks pH __-_ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used _____NA________ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic x None Relict shells Other______None_____ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 8 Detritus sticks wood coarse plant materials
0 Boulder gt256 mm (10rsquorsquo) 0
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 51 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 40 Sand 006-2 mm (gritty) 1 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University
SITE CHARACTERIZATION FIELD DATA SHEET FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Package Plant STATION HLR-2 STREAM CLASS LAT N 39 129580 (upstream) LONG W 078 04900 (upstream) LAT N 39 129581 (downstream) LONG W 078 04822 (downstream) FORM COMPILED BY JW Pangle RIVER BASIN Potomac River INVESTIGATORS JPangle JTovar MDahl B Millholland SRobertson W Bousquet
DATE__19 May 2008_ TIME__1240__ PM
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature _____degC showers (intermittent) Comments __Sunny Day_____________
_5__ cloud cover _0__ ______________________________
x clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x (Significant springs andor seeps) x (Valley) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS
e
f
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence FieldPasture Industrial Obvious sources Agricultural Other ____________ __Upstream Construction and Stream bank Scour Residential __________________________________ Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left Deciduous forest with thick herb and shrub layers bank= vegetation and rocks Right Deciduous forest with thick herb and shrub layers bank=vegetation soil and rocks Indicate the dominant vegetation type(s) and record the dominant species
x Trees x Shrubs Grasses Forbs Dominant species Black Walnut White Ash Hackberry and American Sycamore
INSTREAM FEATURES
Measured reach length 100 m Canopy Cover Estimated stream width _35__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area __350_m2
Estimated stream depth __15__m Proportion of Reach Represented by Stream High water mark __22___m Morphology Types Surface velocity (at thalweg) _035__msec x Riffle__30___ x Run__30___ x Pool__40___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___10____ m2 Density of LWD _______ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __30___ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present__________________________________________________________
WATER QUALITY Temperature __18___degC Water Odors x NormalNone Sewage Specific Conductance __-____ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ____-____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used _____NA____ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other___None_____ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach SubstrateType Characteristic
Composition in Sampling Reach
Bedrock 3 Detritus sticks wood coarse plant materials
2 Boulder gt256 mm (10rsquorsquo) 5
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 70 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 22 Sand 006-2 mm (gritty) 0 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiattrsquos Run LOCATION Rt 664 Bridge STATION HLR-3 STREAM CLASS LAT N 39 12934 (upstream) LONG W 078 05019 (upstream) LAT N 39 12958 (downstream) LONG W 078 04958 (downstream) FORM COMPILED BY Sean Robertson RIVER BASIN Potomac River INVESTIGATORS SRobertson BMillholland WBousquet MDahl JPangle
DATE 28 May 2008 TIME 1127 AM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality 2008
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature __14___degC showers (intermittent) x Trace Comments _______________________
_65 x cloud cover ___ ______________________________
x clearsunny __________________________________
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x (Significant springs andor seeps) x (Valley) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS
g
h
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence Some evidence x FieldPasture Industrial x Obvious sources Agricultural x Other Old Package Plant Downstream x Residential Bridge and Road Power line and Pump station Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left 70 field 30 shrubs and deciduous trees Bank=Soil and vegetation Right Maturing deciduous forest steep slope Bank=Shale Bedrock Indicate the dominant vegetation type(s) and record the dominant species
x Trees x Shrubs x Grasses Forbs Dominant species Sycamore and Red Oaks
INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width __51___m Fully open x Partly open Partly shaded Fully shaded Sampling reach area _510_m2
Estimated stream depth __135___m Proportion of Reach Represented by Stream High water mark __-___m Morphology Types Surface velocity (at thalweg) __20___msec x Riffle_40_ x Run_50_ x Pool 10 Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___0____ m2 Density of LWD ___-____ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation ___50____ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present Brown Algae
WATER QUALITY Temperature __17___degC Water Odors x NormalNone Sewage Specific Conductance ___-___ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ___-_____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used ____NA_______ Clear x Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other____None____ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 15 Detritus sticks wood coarse plant materials
0 Boulder gt256 mm (10rsquorsquo) 15
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 62 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 8 Sand 006-2 mm (gritty) 0 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Unnamed Tributary STATION HLR-4 STREAM CLASS LAT N 39 13035 (upstream) LONG W 078 05129 (upstream) LAT N 39 13000 (downstream) LONG W 078 05086 (downstream) FORM COMPILED BY Marie Dahl RIVER BASIN Potomac River INVESTIGATORS M Dahl HKepple SRobertson AFisher
DATE 31 May 2008 TIME 5 PM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality 2008
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) YES x NO
rain (steady rain) Air Temperature __22___degC showers (intermittent) Comments _______________________
_0__ cloud cover _0__ ______________________________
x clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area __-___ km2 mi2 Montane Significant springs andor seeps x Valley Marsh or swamp Montane amp Valley Mixture of origins Other ___________________
COMMENTS Snowden Bridge development (upstream) and sewer line
i
j
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence x FieldPasture Industrial Obvious sources Agricultural Other ____________ Construction upstream and adjacent x Residential Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left Forested with rocky banks field across silt fence and 2m tree strip Right Forested with some rocky banks and forest through the highway Indicate the dominant vegetation type(s) and record the dominant species
x Trees x Shrubs x Grasses Forbs Dominant species White Ash and Hackberry
INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width ___38__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area __380__m2
Estimated stream depth __12__cm Proportion of Reach Represented by Stream High water mark __2___m Morphology Types Surface velocity (at thalweg) __46___msec x Riffle__25___ x Run__70___ x Pool__5___ Channelized Yes x No Dam Present x Yes No
LARGE WOODY DEBRIS
LWD _______ m2 Density of LWD _______ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation ___NA____ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present___Algae___
WATER QUALITY Temperature __16___degC Water Odors x NormalNone Sewage Specific Conductance __-____ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ___-_____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used ____NA_____ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells x Other___Shale_______ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 0 Detritus sticks wood coarse plant materials
10 Boulder gt256 mm (10rsquorsquo) 5
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 45 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 30 Sand 006-2 mm (gritty) 0 Marl grey shell
fragments 0
Silt 0004-006 mm 20 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Lick Run STATION HLR-5 STREAM CLASS LAT N 39 127517 (upstream) LONG W 078 05685(upstream) LAT N 39 12784 (downstream) LONG W 078 05631 (downstream) FORM COMPILED BY B Millholland RIVER BASIN Potomac River INVESTIGATORS B Millholland JTovar SRobertson MDahl WBousquet JPangle
DATE 29 May 2008 TIME 1040 AM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project
k
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature __15___degC showers (intermittent) Comments _______________________
_5_ X cloud cover _0__ ______________________________
x clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane Significant springs andor seeps x Valley Marsh or swamp Montane amp Valley Mixture of origins Other ___________________
COMMENTS Lick Run appears to be intermittent and may be dry in Fall 2008
l
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence x FieldPasture Industrial Obvious sources Agricultural Other ____________ Adjacent logging stream bank scour Residential Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left 85 young deciduous forest 15 logged Bank=Soil vegetation and cobbles Right Young deciduous forest on 30 slope Bank= Soil vegetation and cobble Indicate the dominant vegetation type(s) and record the dominant species
x Trees Shrubs Grasses Forbs Dominant species Mixed Deciduous INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width __11__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area 110 m2
Estimated stream depth _009__m Proportion of Reach Represented by Stream High water mark __96___m Morphology Types Surface velocity (at thalweg) __01___msec x Riffle__30___ x Run__5___ x Pool__65___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___0____ m2 Density of LWD ___-____ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __70___ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present__________________________________________________________
WATER QUALITY Temperature __14___degC Water Odors x NormalNone Sewage Specific Conductance __-__ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ___-___ Water Surface Oils Slick Sheen Globs Flecks pH __-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used _____NA______ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other_____None_____
SEDIMENT AND SUBSTRATE
Odors Deposits Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other________________ x Other Slightly Salty Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 0 Detritus sticks wood coarse plant materials
5 Boulder gt256 mm (10rsquorsquo) 0
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 75 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 23 Sand 006-2 mm (gritty) 2 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION McCann Farm STATION HLR-6 STREAM CLASS LAT N 39 13076 (upstream) LONG W 078 06539 (upstream) LAT N 39 13063 (downstream) LONG W 078 06473 (downstream) FORM COMPILED BY MDahl RIVER BASIN Potomac River INVESTIGATORS MDahl JPangle SRobertson W Bousqet BMillholland JTovar H Kepple
DATE 30 May 2008 TIME 1050 AM (start)
PROJECT NAME
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) YES x NO
rain (steady rain) Air Temperature __18___degC showers (intermittent) Comments _______________________
_85_x cloud cover _10__ ______________________________
clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x (Significant springs andor seeps) x ( Valley) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS Multi-habitat sampling with D-frame nets and seine
m
n
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial x No evidence Some evidence x FieldPasture Industrial Obvious sources x Agricultural Other ____________ Minor stream bank scour Residential Local Watershed Erosion x None Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left 35 bank grasses scattered trees and some bushes Right High bank with grasses and scattered trees Indicate the dominant vegetation type(s) and record the dominant species
x Trees Shrubs Grasses Forbs Dominant species Black Walnut and Festuca elatior
INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width __2___m Fully open Partly open x Partly shaded Fully shaded Sampling reach area __200_m2
Estimated stream depth __03_m Proportion of Reach Represented by Stream High water mark __1___m Morphology Types Surface velocity (at thalweg) __166_msec x Riffle__0___ x Run__55___ x Pool__45___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___2____ m2 Density of LWD ___-____ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __20_____ x Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present ____Glyceria (grass)sp__________
WATER QUALITY Temperature __16___degC Water Odors x NormalNone Sewage Specific Conductance ___-___ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ____-____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used ______NA___ Clear x Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other__NONE__ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 0 Detritus sticks wood coarse plant materials
5 Boulder gt256 mm (10rsquorsquo) 0
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 27 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 38 Sand 006-2 mm (gritty) 5 Marl grey shell
fragments 0 (lt1)
Silt 0004-006 mm 25 Clay lt 0004 mm (slick) 5 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Appendix B
WATER QUALITY ANALYSIS THROUGH THE VIRGINIA
STREAM CONDITION INDEX (VSCI)
The Virginia Stream Condition Index (VSCI) relies on eight metrics to categorize stream health According to Burton and Gerritsen (2003 p 1-1) this index ldquohellip can be used as a primary indicator of ecosystem health and can identify impairment with respect to the reference (or natural) conditionrdquo These metrics are numeric measures of a biological communityrsquos species composition diversity ecological niches or tolerance to pollution
Table 13 on the next page describes each VSCI metric lists its formula and provides the best standard value (BSV) for that metric Based on a 100-point scale the BSV is the score that reflects optimum conditions for aquatic life ie the best water quality value possible (DEQ 2006b pp 8 33) The table also explains whether the score increases or decreases with environmental stress
As Table 13 indicates each of the eight VSCI metrics is calculated individually Each formula provides a unitless score based on a 100-point scale with 0 as the worst possible score and 100 as the best Any metric that provides a result above 100 is changed to 100 in order for the index to work properly After the eight metrics are calculated they are summed and averaged to provide a final VSCI score on a 100-point scale
Based on statistical analysis of Virginiarsquos streams and their BMI communities the DEQ (2006b p 8) currently uses the following tiers to interpret VSCI scores
VSCI Scores Aquatic Life Use
(ALI) Tiers
lt 42 severely stressed
42-55 moderately stressed
55-63 unknownfair (gray zone)
63-73 good
gt73 excellent
Section 2 of this report presents the results of calculations for the six BMI sampling sites the research team studied in the Hiatt-Lick Run subwatershed in 2008
o
p
1 Total taxa the number of distinct taxa in the sample measures the overall diversity of the macroinvertebrate assemblage The best standard value (BSV) is 189 Score = 100 x (X189) Total taxa decreases with stress 2 EPT taxa the sum of the distinct taxa in the orders Ephemeroptera (mayflies) Plecoptera (stoneflies) and Trichoptera (caddisflies) These orders are known to be sensitive to pollution The best standard value (BSV) is 117 Score = 100 x (X117) EPT taxa decreases with stress 3 Percent Ephemeroptera the percent abundance of individuals in the sample that belong to the order Ephemeroptera (mayflies) The best standard value (BSV) is 477 Score = 100 x (X477) Percent Ephemeroptera decreases with stress 4 Percent Plecoptera + Trichoptera less Hydropsychidae the percent abundance of individuals in the sample that belong to the orders Plecoptera (stoneflies) and Trichoptera (caddisflies) excluding the pollution-tolerant caddisfly family Hydropsychidae The best standard value (BSV) is 562 Score = 100 x (X562) Percent Plecoptera + Trichoptera less Hydropsychidae decreases with stress 5 Percent Scrapers the percent abundance of individuals in the sample whose primary functional mechanism for feeding is grazing on the substrate or periphyton (ie attached algae and associated material) The best standard value (BSV) is 467 Score = 100 x (X467) Percent scrapers decreases with stress 6 Percent Chironomidae the percent abundance of individuals in the sample that are Chironomidae (non-biting midge) larvae and pupae The best standard value (BSV) is 16 Score = 100 x [(100-X)(100-16)] Percent Chironomidae increases with stress 7 Percent Top Two Dominant the percent abundance of the two most frequent taxa present in the sample The best standard value (BSV) is 328 Score = 100 x [(100-X)(100-328)] The Percent Top 2 Dominant increases with stress 8 Hilsenhoff Biotic Index (HBI) an abundance-weighted average pollution tolerance of all the individuals within a sample keyed to the family level Tolerance ratings range from 1 (highly intolerant) to 10 (highly tolerant) The best standard value (BSV) is 29 Score = 100 x [(10-X)(10-29)] The Hilsenhoff Biotic Index (HBI) increases with stress ______________________________________________________________________________ Table 13 Description of Benthic Macroinvertebrate VSCI Metrics Includes responses (increases decreases) of each VSCI metric to degraded water quality conditions Adapted from Burton and Gerritsen (2003 pp 3-15 3-16 6-2) and DEQ (2008)
q
Appendix C
LABORATORY BENCH SHEETS
Shenandoah Universityrsquos Environmental Studies Department developed the Benthic Macroinvertebrate Laboratory Bench Sheet and VSCI Worksheet to record BMI specimen identifications and their VSCI classifications for each sample
This form is based in part on bench sheets developed by the EPA and Virginia DEQ for recording specimen identifications Additional columns on Shenandoah Universityrsquos forms for relative numbers and VSCI classifications make the calculation and verification of VSCI scores easier
Note the column for taxonomistrsquos initials Specimens were identified by students in Shenandoah Universityrsquos research group and then verified or where necessary changed by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality This external review helps assure the validity of the study Originals of these bench sheets with handwritten taxonomistsrsquo initials will be kept on file at Shenandoah Universityrsquos Environmental Studies Department for five years after the completion of this study
r
s
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-1 STATION NAME Mouth LAT N 39deg 12749rsquo (upstream) LONG W 078deg 04209rsquo (upstream) LAT N 39deg 12729rsquo (downstream) LONG W 078deg 04148rsquo (downstream) FORM COMPILED BY Jeremy Tovar W S Bousquet RIVER BASIN Potomac River INVESTIGATORS Marie Dahl Brandon Millholand J W Pangle Sean Robertson Jeremy Tovar Woodward Bousquet Ben Bousquet
Collection date 21 May 2008 Sheet completion date 12 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Hirudinea 3 33 JT BVW PR 8 SP Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Coenagrionidae 2 22 BVW PR 9 CR Ephemeroptera (mayflies) Caenidae 1 11 JT BVW CG 6 SP Ephemerllidae 4 44 JT BVW CG 2 CR Heptageniidae 5 55 JT BVW SC 3 CG Isonychiidae 1 11 BVW CF 3 CG Plecoptera (stoneflies) Perlidae 14 154 JT BVW PR 2 CR Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Corydalidae 1 11 JT BVW PR 5 CR Coleoptera (beetles) Elmidae 24 264 I JT BVW SC 4 CG Dytiscidae 4 44 JT BVW PR 6 GN Psephenidae 3 33 JT BVW SC 4 CG Diptera (true flies) Tipulidae 3 33 JT BVW SH 4 BU Chironomidae 26 286 JT BVW CG 6 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
t
Other Total Individuals 91 1001 Total Taxa 13
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
u
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-1 STATION NAME Mouth LAT N 39deg 12749rsquo (upstream) LONG W 078deg 04209rsquo (upstream) LAT N 39deg 12729rsquo (downstream) LONG W 078deg 04148rsquo (downstream) FORM COMPILED BY Jeremy Tovar W S Bousquet RIVER BASIN Potomac River INVESTIGATORS Brandon Millholand JW Pangle Jeremy Tovar Heather Kepple
Collection date 10 October 2008 Sheet completion date 12 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Oligochaeta 3 35 JT BVW CG 8 BU Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Coenagrionidae 2 23 JT BVW PR 9 CR Ephemeroptera (mayflies) Heptageniidae 12 140 JT BVW SC 3 CG Isonychiidae 2 23 BVW CF 3 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Hydropsychidae 16 186 JT BVW CF 6 CG Philopotamidae 31 360 JT BVW CF 2 CG Hemiptera (true bugs) (Veliidae) (5) - JT BVW ( ) Megaloptera (dobsonflies etc) Corydalidae 10 116 JT BVW PR 5 CR Coleoptera (beetles) Dytiscidae 1 12 JT BVW PR 6 GN Psephenidae 7 81 JT BVW SC 4 CG Diptera (true flies) Tipulidae 2 23 BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
v
Other Total Individuals 86
(91) 999
Total Taxa 10 (11)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009 Veliidae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
w
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-2 STATION NAME Package Plant LAT N 39deg 12958rsquo (upstream) LONG W 078deg 04900 (upstream) LAT N 39deg 12958rsquo (downstream) LONG W 078deg 04822 (downstream) FORM COMPILED BY JW Pangle W Bousquet RIVER BASIN Potomac River INVESTIGATORS JW Pangle S Robertson J Tovar M Dahl B Millholland W Bousquet
Collection date 29 May 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Plecoptera (stoneflies) Perlidae 11 136 I JP BVW PR 1 CR Trichoptera (caddisflies) Hydropsychidae 10 123 I JP BVW CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 14 173 I JP BVW SC 4 CG Diptera (true flies) Chironomidae 32 395 I JP BVW CG 6 BU Simuliidae 14 173 I JP BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
x
Other Total Individuals 81 1000 Total Taxa 5
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-2 STATION NAME Package Plant LAT N 39deg 12958rsquo (upstream) LONG W 078deg 04900 (upstream) LAT N 39deg 12958rsquo (downstream) LONG W 078deg 04822 (downstream) FORM COMPILED BY JW Pangle W Bousquet RIVER BASIN Potomac River INVESTIGATORS JW Pangle Brandon Millholland Jeremy Tovar Heather Kepple
Collection date 10 October 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Heptageniidae 40 392 I WBJP SC 3 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Hydropsychidae 7 69 I JP WB BVW CF 6 CG Philopotamidae 28 275 I JP WB BVW CF 2 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Corydalidae 6 59 I JP WB BVW PR 5 CR Coleoptera (beetles) Psephenidae 7 69 I JP WB BVW SC 4 CG Elmidae 8 78 I JP WB BVW SC 4 CG Diptera (true flies) Tipulidae 6 59 I JP WB BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
y
z
Other Total Individuals 102 1001 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-3 STATION NAME Rt 664 Bridge LAT N 39deg 12934rsquo (upstream) LONG W 078deg 05019rsquo (upstream) LAT N 39deg 12951rsquo (downstream) LONG W 078deg 04958rsquo (downstream) FORM COMPILED BY S Robertson W Bousquet RIVER BASIN Potomac River INVESTIGATORS SRobertson BMillholland WBousquet MDahl JPangle
Collection date 28 May 2008 Sheet completion date 11 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Leptophlebiidae 1 15 I SGR BVW CG 2 CR Baetidae 1 15 I BVW CG 5 CG Plecoptera (stoneflies) Perlidae 3 46 I SGR BVW PR 1 CR Nemouridae 1 15 I BVW SH 3 CR Trichoptera (caddisflies) Hydropsychidae 5 77 I SGR BVW CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 27 415 I SGR BVW SC 4 CG Psephenidae 2 31 A SGR BVW SC 4 CG Diptera (true flies) Simuliidae 5 77 I SGR BVW CF 6 CG Chironomidae 20 308 I SGR BVW CG 6 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
aa
bb
Other Total Individuals 65 999 Total Taxa 9
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-3 STATION NAME Rt 664 Bridge LAT N 39deg 12934rsquo (upstream) LONG W 078deg 05019rsquo (upstream) LAT N 39deg 12951rsquo (downstream) LONG W 078deg 04958rsquo (downstream) FORM COMPILED BY S Robertson RIVER BASIN Potomac River INVESTIGATORS S Robertson M Dahl A Fisher H Kepple
Collection date 10 Oct 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Heptageniidae 2 20 I SGR SC 3 CG Isonychiidae 2 20 I BVW CF 2 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Philopotamidae 36 367 I SGR CF 2 CG Hydropsychidae 47 480 I SGR CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 2 20 A SGR SC 4 CG Psephenidae 8 82 A SGR SC 4 CG Diptera (true flies) Tabanidae 1 10 I SGR PR 7 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
cc
dd
Other Total Individuals 98 999 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-4 STATION NAME Unnamed Tributary LAT (upstream) N 39deg 13035rsquo LONG (upstream) W 078deg 05129rsquo LAT (downstream) N 39deg 13000rsquo LONG (downstream) W 078deg 5086rsquo FORM COMPILED BY A Fisher M Dahl rev W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson A Fisher H Kepple
Collection date 31 May 2008 Sheet completion date rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Flatworms (Turbellaria) Worms (Oligochaeta) Naididae 1 14 - BVW CG 8 BU Leeches (Hirudinea) Snails (Gastropoda) Clams (Bivalvia) Isopods (Isopoda) Asellidae 3 42 A AF BVW CG 8 SP Scuds (Amphipoda) Crayfish Shrimp (Decapoda) Water Mites (Hydracarina) Dragonflies (Odonata Anisoptera) Damselflies (Odonata Zygoptera) Mayflies (Ephemeroptera) Stoneflies (Plecoptera) Perlidae 2 28 I AF BVW PR 1 CR Caddisflies (Trichoptera) True Bugs (Hemiptera) Corixidae 1 14 I AF BVW MP 9 DV Dobsonflies (Megaloptera) Beetles (Coleoptera) Dytiscidae 1 14 I AF BVW PR 6 GN True Flies (Diptera) Chironomidae 61 847 I AF BVW CG 6 BU Simuliidae 3 42 I AF BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
ee
ff
Other Total Individuals 72 1001 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-4 STATION NAME Unnamed Tributary LAT (upstream) N 39deg 13035rsquo LONG (upstream) W 078deg 05129rsquo LAT (downstream) N 39deg 13000rsquo LONG (downstream) W 078deg 5086rsquo FORM COMPILED BY Andrew G M Fisher W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson A Fisher H Kepple
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Flatworms (Turbellaria) Worms (Oligochaeta) Lumbriculidae 2 32 A AF BVW CG 8 BU Leeches (Hirudinea) Snails (Gastropoda) Lymnaeidae 1 16 A BVW CG 9 GN Clams (Bivalvia) Isopods (Isopoda) Scuds (Amphipoda) Crayfish Shrimp (Decapoda) Cambaridae 1 16 A AF BVW GN 5 GN Water Mites (Hydracarina) Dragonflies (Odonata Anisoptera) Damselflies (Odonata Zygoptera) Calopterygidae 4 63 I BVW PR 7 CL Mayflies (Ephemeroptera) Tricorythidae 1 16 I BVW CG 5 SP Stoneflies (Plecoptera) Caddisflies (Trichoptera) Phryganeidae 4 63 I AF BVW SH 4 CR Hydropsychidae 6 95 I AF BVW CF 6 CG I True Bugs (Hemiptera) Corixidae 8 127 I AF BVW MP 9 DV (Veliidae ) (7) - I AF BVW ( ) Dobsonflies (Megaloptera) Sialidae 3 48 I AF BVW PR 7 SP Corydalidae 2 32 I AF BVW PR 5 CR Beetles (Coleoptera) Haliplidae 1 16 A AF BVW MP 8 GN Dysticidae 7 111 I AF BVW PR 6 GN Hydrophilidae 1 16 A AF BVW PR 8 GN Elmidae 3 48 I BVW SC 4 CG True Flies (Diptera) Chironomidae 8 127 I AF BVW CG 6 BU Simuliidae 7 111 I AF BVW CF 6 CG Tipulidae 4 63 I AF BVW SH 4 GU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
gg
hh
Other Total Individuals 63
(70) 1000
Total Taxa 17 (18)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Veliidae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-5 STATION NAME Lick Run LAT N 39deg 12752rsquo (upstream) LONG W 078deg 05685rsquo (upstream) LAT N 39deg 12784rsquo (downstream) LONG W 078deg 05631rsquo (downstream) FORM COMPILED BY Brandon Millholland W Bousquet RIVER BASIN Potomac River INVESTIGATORS J Pangle S Robertson W Bousquet JTovar M Dahl
Collection date 29 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Gomphidae 1 15 I BVW PR 3 BU Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Leptophlebiidae 11 167 A LBM BVW CG 2 CR Plecoptera (stoneflies) Perlidae 10 152 A LBM BVW PR 1 CR Perlodiae 1 15 A LBM BVW PR 2 CR Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 5 76 A LBM BVW SC 4 CG Elmidae 2 30 I LBM BVW SC 4 CG Diptera (true flies) (Culicidae) (3) - P LMB BVW ( ) Chironomidae 23 348 I LBM BVW CG 6 BU Simuliidae 13 197 I LBM BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
ii
jj
Other Total Individuals 66
(69) 1000
Total Taxa 7 (8)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Culicidae (Diptera) pupae not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl J Pangle S Robertson W Bousquet B Millholland J Tovar H Kepple
Collection date 30 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 1 12 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Asellidae 114 965 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Plecoptera (stoneflies) Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 2 23 A MRD BVW SC 4 CG Diptera (true flies)
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
kk
ll
Other Total Individuals 117 1000 Total Taxa 3
Notes Subsample taken 29 October 2009 All taxonomic determinations by MRD were confirmed by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson K McCann A Fisher H Kepple A Barrett
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 2 17 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Sphaeriidae 2 17 I MRD BVW CF 8 BU Isopoda (isopods) Asellidae 99 853 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Calopterygidae 2 17 A MRD BVW PR 7 CL Ephemeroptera (mayflies) Leptophlebiidae 2 17 I MRD BVW CG 2 CR Heptageniidae 4 34 A MRD BVW SC 3 CG Plecoptera (stoneflies) Perlidae 1 09 A MRD BVW PR 1 CR Trichoptera (caddisflies) Hemiptera (true bugs) (Gerridae) (1) - A MRD BVW ( ) Corixidae 1 09 A MRD BVW PR 5 CR Megaloptera (dobsonflies etc) Coleoptera (beetles) Dryopidae 1 09 A MRD BVW SC 5 CG Diptera (true flies) Tipulidae 2 17 I BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
mm
nn
Other Total Individuals 116
(117) 999
Total Taxa 10 (11)
Notes Subsample taken 6 November 2008 Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009 Gerridae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
- Disclaimer ii
- Title Page iii
- Acknowledgements v
- Table of Contents vii
- SECTION 2 RESULTS DATA TABLES 7
- OVERVIEW AND FINDINGS
- Introduction
- RESULTS DATA TABLES
- REFERENCES
- FIELD DATA SHEETS
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- SubstrateType
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- WATER QUALITY ANALYSIS
- THROUGH THE VIRGINIA
- STREAM CONDITION INDEX (VSCI)
- LABORATORY BENCH SHEETS
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
This report may be cited as follows Dahl Marie R Woodward S Bousquet L Brandon Millholland J W Pangle Sean G Robertson Jeremy D Tovar Jared B Davis and Andrew G M Fisher 2009 (online edition 2010) Biological Monitoring in Hiatt and Lick Runs Opequon Creek Watershed Frederick County Virginia 2008 Baseline Data and a Monitoring Framework Winchester Virginia Environmental Studies Program Shenandoah University
iv
ACKNOWLEDGEMENTS
Many individuals and organizations provided time support and expertise for this research
study Although we cannot list everyone by name we would like to express our sincere gratitude to all who made the project possible including Jim Lawrence ndash Coordinator Opequon Targeted Watershed Project Property owners along Hiatt Run and Lick Run
Mike Hoffman Leslie Johns The McCann-Slaughter Family Ervin and Barbara Simons Tonie Wallace-Aitken and Greg Aitken Historic Jordan Springs Event and Cultural Centre
Billy van Wart ndash Regional Aquatic Biologist Virginia Department of Environmental Quality Heather Kepple ndash Technician Opequon Targeted Watershed Project Field Assistance Andrew Barrett
Ben Bousquet Katherine McCann
Financial Support
Opequon Creek Targeted Watershed Grant Dr and Mrs Charles Leeper An anonymous family foundation Shenandoah University
v
vi
TABLE OF CONTENTS page
Disclaimer ii Title Page iii Acknowledgements v Table of Contents vii SECTION 1 OVERVIEW AND FINDINGS 1 Introduction 1 Methods and Analysis 3 Findings 3 Future Research 5 SECTION 2 RESULTS DATA TABLES 7 Site HLR-1 Mouth 7 Site HLR-2 Package Plant 8 Site HLR-3 Route 664 Bridge 9 Site HLR-4 Unnamed Tributary 10 Site HLR-5 Lick Run 11 Site HLR-6 McCann Farm 12 REFERENCES 13 APPENDICES 15 Appendix A Field Data Sheets Appendix B Water Quality Analysis through the Virginia Stream Condition Index (VSCI) Appendix C Laboratory Bench Sheets Note Photographs of the six sampling sites appear on a CD provided separately to the grant administrator
vii
viii
Section 1
OVERVIEW AND FINDINGS
Introduction
This report presents baseline water quality data for Hiatt Run and Lick Run -- termed the Hiatt-Lick Run subwatershed in this study Located in Frederick County Virginia Hiatt and Lick Runs are tributaries of Opequon Creek The Opequon in turn empties into the Potomac River and then the Chesapeake Bay The investigation was conducted by a Shenandoah University research team in 2008 Researchers used benthic macroinvertebrates (BMIs) as biological indicators of water quality This report presents an overview of the study followed by summary tables and a list of references The Appendix contains data sheets and a description of the data analysis These data will form part of the baseline water quality assessment that is being undertaken through the Opequon Creek Targeted Watershed Grant The grant administered by Jim Lawrence reflects a broad-based partnership that includes Virginia Tech Shenandoah University the Frederick-Winchester Service Authority The Opequon Watershed (TOW) citizens organization as well as federal state and local governments community groups and business interests Grant partners will use best management practices (BMPs) to reduce nutrient runoff and address other pollution problems in the Opequon Creek watershed Since 1996 Opequon Creek has appeared on the impaired waters list prepared by the Virginia Department of Environmental Quality (DEQ) The Hiatt-Lick Run subwatershed was selected for this study because it represents changes that are typical of the Opequon Creek watershed These changes include new commercial and residential developments as well as the implementation of water quality improvement practices such as tree planting in riparian zones fences to prevent livestock from entering streams and replacing outdated sewage treatment facilities such as a package plant on lower Hiatt Run
Seven students from Shenandoah Universityrsquos Environmental Studies Department conducted this study from May through December 2008 under the direction of Woodward Bousquet Professor of Environmental Studies and Biology The research team followed procedures described in the Rapid Bioassessment Protocols (RBPs) of the US Environmental Protection Agency (Barbour et al 1999) and the Virginia Stream Condition Index (VSCI) The VSCI provides an evaluation of water quality based on a streamrsquos benthic macroinvertebrate community (Burton and Gerritsen 2003 DEQ 2006b and 2008)
In consultation with Billy van Wart of the DEQ and grant administrator Jim Lawrence the Shenandoah University researchers selected six representative sampling sites in the Hiatt-Lick Run subwatershed Each site was given a name and an HLR (for Hiatt-Lick Run) code Figure 1 maps their positions while their characteristics and coordinates appear in the field data sheets (see Appendix A) These sites were established according to EPA and DEQ guidelines that generally call for a 100-meter-long stream reach with at least three riffle zones a width sufficient to accommodate a 1-meter seine and a substrate composed of least 35 cobbles
Specific locations of the sampling sites on Hiatt and Lick Runs were selected to reflect different watershed positions and changing land uses See Figure 1 Further information about each sampling site appears in the field data sheets (Appendix A)
1
Figure 1 Sampling Sites in the Hiatt-Lick Run Subwatershed of Opequon Creek Frederick County Virginia Scale 1 centimeter = 500 meters
HLR-1 (Mouth) measures water quality at the lowest point of the subwatershed as Hiatt Run flows into Opequon Creek Forests cover the immediate riparian zones on both sides of Hiatt Run This site will be used in future years by the DEQ for BMI monitoring HLR-2 (Package Plant) is situated on Hiatt Run just below the outflow pipe of an outdated package plant that formerly treated sewage from the Jordan Springs area During the study period the package plant was taken off-line and a pumping station was constructed The Frederick-Winchester Service Authority now treats this sewage at its Opequon Water Reclamation Facility HLR-3 (Route 664 Bridge) was chosen because it is located upstream from the package plant but downstream of the confluence of Hiatt Run and Lick Run HLR-4 (Unnamed Tributary) is located downstream of the Snowden Bridge residential development which has been under construction The area immediately upstream of this sampling site was being graded by heavy equipment during the May 2008 sampling time HLR-5 (Lick Run) the researchers learned dries up in late summer It had a modest but constant flow during when the May 2008 sample was taken HLR-6 (McCann Farm) represents the headwater areas of the Hiatt-Lick Run subwatershed of Opequon Creek The McCanns who graze cattle in their pastures that surround Hiatt Run here have been working with the Potomac Conservancy to implement Best Management Practices
2
(BMPs) to improve water quality These practices include fencing cattle out of the stream and spring runs and planting more trees in the riparian zone
Methods and Analysis
The research team established a sampling plan and then sampled according to methods the DEQ developed for the VSCI Using a 1 meter x 1 meter seine samples from 2m2 of substrate in riffle zones were to be collected twice at each site once in May 2008 and once in October 2008 At five of the six sites stream characteristics permitted the standard single-habitat sampling procedure At the McCann Farm (Site HLR-6) researchers needed to use multi-habitat sampling See Barbour et al (1999 pp 7-3 ff) for further descriptions of these two methods
All six sites were sampled in May 2008 Low-water conditions in October 2008 prevented the Shenandoah University group from collecting BMIs at Lick Run (HLR-5) that month but the other five sites were sampled successfully As a result the research team obtained eleven samples for analysis in this study Samples were preserved in ethanol for subsequent identification
DEQ procedures require subsampling when the number of BMIs in a given sample exceeds 100 All eleven samples in this study met that criterion Consequently researchers used a Caton tray to produce random subsamples of 90 to 110 specimens from each sample During the analysis that followed some specimens in the subsamples were rejected either because they turned out to be terrestrial species or because they were in the wrong life stage (eg pupae) In most cases the researchers identified specimens to the family level Primary taxonomic references were A Guide to Common Freshwater Invertebrates of North America (Voshell 2002) Fresh-Water Invertebrates of the United States (Pennak 1953) and An Introduction to the Aquatic Insects of North America (Merritt and Cummins 1996) DEQ Regional Aquatic Biologist Billy van Wart reviewed verified and where necessary corrected the identifications
The investigators then used the Virginia Stream Condition Index (VSCI) to produce water quality scores based on the BMIs collected A VSCI score is based on eight different measurement formulas called metrics Each metric is a numeric measure of a biological communityrsquos species composition diversity ecological niches or tolerance to pollution When combined into a single multimetric VSCI score the response of the benthic community to water conditions can be evaluated For further information and worksheets see Appendices B and C respectively
Findings
Table 1 presents the VSCI scores for the eleven samples collected in the Hiatt-Lick Run subwatershed in 2008 Since a sample year consists of two samples the mean scores for each site for the entire year are also included As the table shows
bull None of the 11 samples was rated excellent bull Only 1 of the 11 samples was rated as good bull Only 2 of the 11 samples were rated fair (ie in the ldquogrey zonerdquo between impaired and
unimpaired) bull The remaining 8 samples were in either moderately stressed or severely stressed
condition
3
Site Name and Code Sampling
Date of
TaxaVCSI Score
Water Quality Rating
Mouth (HLR-1) 21 May 2008 13 571 Fair (Gray Zone)
Mouth (HLR-1) 10 Oct 2008 10 608 Fair (Gray Zone)
site average for 2008 590 Fair (Gray Zone)
Package Plant (HLR-2) 29 May 2008 5 370 Severely Stressed
Package Plant (HLR-2) 10 Oct 2008 7 674 Good
site average for 2008 522 Moderately Stressed
Route 664 Bridge (HLR-3) 28 May 2008 9 485 Moderately Stressed
Route 664 Bridge (HLR-3) 10 Oct 2008 7 469 Moderately Stressed
site average for 2008 477 Moderately Stressed
Unnamed Tributary (HLR-4) 31 May 2008 7 173 Severely Stressed
Unnamed Tributary (HLR-4) 10 Oct 2008 17 476 Moderately Stressed
site average for 2008 325 Severely Stressed
Upper Lick Run (HLR-5) 29 May 2008 7 455 Moderately Stressed
site average for 2008 455 Moderately Stressed
McCann Farm (HLR-6) 30 May 2008 3 190 Severely Stressed
McCann Farm (HLR-6) 10 Oct 2008 10 316 Severely Stressed
site average for 2008 253 Severely Stressed
Summary 6 sites 11 samples
36 BMI taxa
VSCI = Virginia Stream Condition Index see Burton and Gerritsen (2003) and Virginia Department of Environmental Quality (2006b and 2008) Table 1 Summary Table of Virginia Stream Condition Index (VSCI) Scores and Water Quality Ratings for Sites in the Hiatt-Lick Run Subwatershed of Opequon Creek Frederick County Virginia 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
4
Improvements in water quality ratings at three sites between May and October suggest that BMPs and other changes may already be having some positive effects on water quality At the Package Plant (Site HLR-2) the VSCI score rose dramatically from 370 (severely stressed) to 674 (good) The small-scale sewage treatment plant as noted above was taken off-line during this period Similarly water quality at the Unnamed Tributary (HLR-4) increased from 173 (severely stressed) to 476 (moderately stressed) Adjacent construction was completed over the summer and grass was planted on the property Management practices on the McCann Farm (HLR-6) may be responsible for improving conditions for aquatic life in Hiatt Runrsquos headwaters Even though the water quality was still rated as severely stressed in October the VSCI score rose from 190 to 316 at this sampling site Further studies are needed to determine whether the changes noted in 2008 persist into the future In 2003 the DEQ began a Total Maximum Daily Load (TMDL) process for Opequon Creek as required by the Water Quality Act of 1987 The TMDL approach establishes a ldquopollution budgetrdquo for each impaired stream for each type of impairment and for each responsible pollutant In conjunction with the DEQ and the Virginia Department of Conservation and Recreation (DCR) Virginia Polytechnic Institutersquos Department of Biosystems Engineering developed a TMDL implementation plan (Opequon Creek IP Steering Committee et al 2006) Monitoring the BMI communities of Opequon Creek and its tributaries is essential to evaluating the progress of the proposed water quality improvement projects
Future Research The relatively small Hiatt-Lick Run subwatershed provides several case studies that can help assess the effectiveness of various water quality management practices not only in Opequon Creek but also in other watersheds in the Chesapeake Bay region and beyond In supporting that goal this study makes two principal contributions 1 It provides baseline data to measure the improvement or degradation of the Hiatt-Lick Run section of the Opequon Creek watershed and 2 It establishes a monitoring framework for the long-term biological monitoring of water quality and the evaluation of best management practices (BMPs) This framework consists of
bull Sampling Sites ndash six stream reaches in the Hiatt-Lick Run subwatershed selected as sampling sites according to EPA and DEQ specifications
bull Data Collection Protocols ndash based on the EPArsquos Rapid Bioassessment Protocols (RBPs)
(Barbour et al 1999) and the DEQrsquos Virginia Stream Index (VSCI) (Burton and Gerritsen 2003 DEQ 2006b and 2008) and
bull Analytical Procedures ndash contained in the Virginia Stream Condition Index (VSCI see
Burton and Gerritsen 2003 DEQ 2006b pp 8 and 33 and DEQ 2008 pp 29-34)
5
6
Section 2
RESULTS DATA TABLES
Site HLR-1 Mouth
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 13 189 6872 EPT taxa 5 117 4273 Ephemeroptera 121 477 2544 Plec +Tric less Hydropsych 154 562 2745 Scrapers 352 467 7536 Chironomidae 286 16 7267 Top 2 Dominant 550 328 6708 Hilsenhoff Biotic Index (HBI) 45 29 775VSCI Score 571
According to the DEQrsquos water quality standards aquatic life at this site is fair (gray zone) See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 2 VSCI Scores for Mouth (HLR-1) Site in Hiatt-Lick Run Sampled on 21 May 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 10 189 5292 EPT taxa 4 117 3423 Ephemeroptera 163 477 3424 Plec +Tric less Hydropsych 360 562 6415 Scrapers 221 467 4736 Chironomidae 00 16 10007 Top 2 Dominant 546 328 6768 Hilsenhoff Biotic Index (HBI) 39 29 859VSCI Score 608
According to the DEQrsquos water quality standards aquatic life at this site is fair (gray zone) See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 3 VSCI Scores for Mouth (HLR-1) Site in Hiatt-Lick Run Sampled 10 October 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
7
Site HLR-2 Package Plant
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 5 189 2642 EPT taxa 2 117 1713 Ephemeroptera 00 477 004 Plec +Tric less Hydropsych 110 562 1965 Scrapers 173 467 3706 Chironomidae 395 16 6157 Top 2 Dominant 568 328 6438 Hilsenhoff Biotic Index (HBI) 50 29 704VSCI Score 370
According to the DEQrsquos water quality standards aquatic life at this site is severely stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 4 VSCI Scores for Package Plant (HLR-2) Site in Hiatt-Lick Run Sampled 29 May 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 7 189 3702 EPT taxa 34 117 2563 Ephemeroptera 392 477 8214 Plec +Tric less Hydropsych 275 562 4895 Scrapers 539 467 10006 Chironomidae 00 16 10007 Top 2 Dominant 667 328 4968 Hilsenhoff Biotic Index (HBI) 32 29 958VSCI Score 674
According to the DEQrsquos water quality standards aquatic life at this site is good See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 5 VSCI Scores for Package Plant (HLR-2) Site in Hiatt-Lick Run Sampled 10 October 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
8
Site HLR-3 Route 664 Bridge
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 9 189 4762 EPT taxa 5 117 4273 Ephemeroptera 30 477 634 Plec +Tric less Hydropsych 61 562 1095 Scrapers 446 467 9556 Chironomidae 308 16 7037 Top 2 Dominant 723 328 4128 Hilsenhoff Biotic Index (HBI) 48 29 732VSCI Score 485
According to the DEQrsquos water quality standards aquatic life at this site is moderately stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 6 VSCI Scores for Route 664 Bridge (HLR-3) Site in Hiatt-Lick Run Sampled 28 May 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 7 189 3702 EPT taxa 4 117 3423 Ephemeroptera 40 477 844 Plec +Tric less Hydropsych 367 562 6535 Scrapers 122 467 2616 Chironomidae 00 16 10007 Top 2 Dominant 847 328 2288 Hilsenhoff Biotic Index (HBI) 42 29 817VSCI Score 469
According to the DEQrsquos water quality standards aquatic life at this site is moderately stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 7 VSCI Scores for Route 664 Bridge (HLR-3) Site in Hiatt-Lick Run Sampled 10 October 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
9
Site HLR-4 Unnamed Tributary
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 7 189 3702 EPT taxa 1 117 853 Ephemeroptera 00 477 004 Plec +Tric less Hydropsych 28 562 495 Scrapers 00 467 006 Chironomidae 847 16 1557 Top 2 Dominant 889 328 1658 Hilsenhoff Biotic Index (HBI) 60 29 563VSCI Score 173
According to the DEQrsquos water quality standards aquatic life at this site is severely stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 8 VSCI Scores for Unnamed Tributary (HLR-4) Site in Hiatt-Lick Run Sampled 31 May 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 17 189 8992 EPT taxa 3 117 2563 Ephemeroptera 16 477 344 Plec +Tric less Hydropsych 63 562 1125 Scrapers 48 467 1036 Chironomidae 127 16 8877 Top 2 Dominant 254 328 10008 Hilsenhoff Biotic Index (HBI) 63 29 521VSCI Score 476
According to the DEQrsquos water quality standards aquatic life at this site is moderately stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 9 VSCI Scores for Unnamed Tributary (HLR-4) Site in Hiatt-Lick Run Sampled 10 October 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
10
Site HLR-5 Lick Run
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 7 189 3702 EPT taxa 3 117 2563 Ephemeroptera 167 477 3504 Plec +Tric less Hydropsych 167 562 2975 Scrapers 106 467 2276 Chironomidae 348 16 6637 Top 2 Dominant 545 328 6778 Hilsenhoff Biotic Index (HBI) 43 29 803VSCI Score 455
According to the DEQrsquos water quality standards aquatic life at this site is moderately stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 10 VSCI Scores for Lick Run (HLR-5) Site in Hiatt-Lick Run Sampled 29 May 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
Note
Water conditions at the Lick Run (HLR-5) site were too low in October 2008 to permit a sample to be taken
11
Site HLR-6 McCann Farm
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 3 189 1592 EPT taxa 0 117 003 Ephemeroptera 00 477 004 Plec +Tric less Hydropsych 00 562 005 Scrapers 23 467 496 Chironomidae 00 16 10007 Top 2 Dominant 988 328 188 Hilsenhoff Biotic Index (HBI) 79 29 296VSCI Score 190
According to the DEQrsquos water quality standards aquatic life at this site is severely stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 11 VSCI Scores for McCann Farm (HLR-6) Site in Hiatt-Lick Run Sampled 30 May 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 10 189 5292 EPT taxa 3 117 2563 Ephemeroptera 51 477 1074 Plec +Tric less Hydropsych 09 562 165 Scrapers 43 467 926 Chironomidae 00 16 10007 Top 2 Dominant 887 328 1688 Hilsenhoff Biotic Index (HBI) 75 29 352VSCI Score 315
According to the DEQrsquos water quality standards aquatic life at this site is severely stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 12 VSCI Scores for McCann Farm (HLR-6) Site in Hiatt-Lick Run Sampled 10 October 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
12
REFERENCES
Barbour Michael T Jeroen Gerritsen Blaine D Snyder and James B Stribling 1999 Rapid bioassessment protocols for use in streams and wadeable rivers periphyton benthic macroinvertebrates and fish Second edition EPA 841-B-99-002 Washington DC US Environmental Protection Agency Office of Water Available online at httpwwwepagovOWOWmonitoringbioasspshtml Burton June and Jeroen Gerritsen 2003 A stream condition index for Virginia non-coastal streams Prepared for the US EPA Office of Science and Technology US EPA Region 3 Environmental Services Division and Virginia Department of Environmental Quality Owings Mills Maryland Tetra Tech Inc Available online at httpwwwdeqvirginiagovwatermonitoringpdf vastrmconpdf Hessler Rachel A Andrea R Martin Heidi D Ruggeri William B Seymour Jr and Woodward S Bousquet 2006 Water quality assessment using benthic macroinvertebrates A study and compilation Winchester Virginia Environmental Studies Program Shenandoah University Occasional publication no 10 Igoe Robert 2006 DEQ identifies nine impaired streams in Clarke Frederick The Winchester Star 15 July B1 Jackson Charlie 2005 Survey targets Opequon cleanup The Winchester Star 19 August B1 Johnston Elizabeth A and Woodward S Bousquet 2003 What happens when it rains A study of water quality in Abrams Creek and Town Run Winchester and Frederick County Virginia Winchester Virginia Environmental Studies Program Shenandoah University Occasional publication no7 Merritt Richard W and Kenneth W Cummins 1996 An introduction to the aquatic insects of North America Dubuque Iowa KendallHunt Publishing Company Opequon Creek IP Steering Committee Virginia Tech Department of Biological Systems Engineering and Center for TMDL and Watershed Studies University of Virginia Institute for Environmental Negotiation and West Virginia University Division of Resource Management 2006 Opequon Creek watershed TMDL implementation plan Blacksburg Virginia Virginia Tech Department of Biological Systems Engineering Available online at httpwwwtmdlbsevteduoutreachC82 Pennak Robert W 1953 Fresh-water invertebrates of the United States New York New York The Ronald Press Company Rosenburg David M and Vincent H Resh 1993 Freshwater biomonitoring and benthic macroinvertebrates New York New York Chapman amp Hall Shenandoah University Public Relations Office 2002 Data collected by SU and its partners helps identify impaired water Winchester Virginia Available online at httpwwwsuedutemp_ newscfmurlnum=32 Thorp James H and Alan P Covich 1991 Ecology and classification of North American freshwater invertebrates San Diego California Academic Press Inc
13
US Environmental Protection Agency 1997 Volunteer stream monitoring a methods manual EPA 841-B-97-003 Washington DC US Environmental Protection Agency Office of Water Van Meter Val 2003 Opequon Creek pollution limits to be established The Winchester Star 4 April B1 Virginia Department of Environmental Quality 2006a Virginia 305(b)303(d) water quality integrated report to Congress and the EPA Administrator for the period January 1 2000 to December 31 2004 Richmond Virginia Virginia Department of Environmental Quality Available online at httpwwwdeqvirginiagovwqair2006html __________ 2006b Using probabilistic data to validate the non-coastal Virginia Stream Condition Index (draft) VDEQ technical bulletin WQA2006-001 Virginia Department of Environmental Quality Available online at httpwwwloudounwatershedorgstream_assessmentreference_documents VA_DEQ_SCI_Validationpdf __________ 2008 Biological monitoring program quality assurance project plan for wadeable streams and rivers Richmond Virginia Virginia Department of Environmental Quality Update available online at httpwwwdeqstatevausbiomonitoringdocumentsBioMonQAPP_13Aug2008pdf Virginia Tech Aquatic Entomology Laboratory 2005 Taxa dictionary [Excel file] Blacksburg Virginia Virginia Tech Department of Biological Systems Engineering 2003a Opequon watershed TMDLs for benthic impairments Abrams Creek and Lower Opequon Creek Frederick and Clarke Counties Virginia Prepared for the Virginia Department of Environmental Quality and Virginia Department of Conservation and Recreation Blacksburg Virginia Available online at httpwwwdeqstate vaustmdlapptmdlsshenrvrabropebcpdf Voshell J Reese Jr 2002 A guide to common freshwater invertebrates of North America Blacksburg Virginia The McDonald amp Woodward Publishing Company
14
APPENDICES
15
16
a
Appendix A
FIELD DATA SHEETS
This appendix presents a Site Characterization Field Data Sheet for each of the six sampling reaches established on Hiatt Run and Lick Run for this study They were filled out during the sampling runs made in May 2008
These forms were adapted by Shenandoah University from forms used by the EPA and Virginia DEQ for recording characteristics of sampling sites
b
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Mouth STATION _HLR-1_____ STREAM CLASS LAT N 39 12749 (upstream) LONG W 078 04209(upstream) LAT N 39 12729 (downstream) LONG W 078 04148 (downstream) FORM COMPILED BY Jeremy Tovar RIVER BASIN Potomac River INVESTIGATORS JPangle B Millholland MDahl SRobertson W Bousquet JTovar BBousquet
DATE May 21 2008_________ TIME_250 PM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature __24___degC showers (intermittent) Comments _______________________
___ cloud cover _0_ ______________________________
X clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x ( Significant springs andor seeps) x (Valley ) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS
c
d
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence x FieldPasture Industrial Obvious sources Agricultural Other ____________ Stream scour steep slope erosion x Residential Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left Field and young thin deciduous forest 100 Bank=Grass cobble and shrub Right 90 maturing deciduous forest 45 shale slope Bank=Bedrock soil and herbs Indicate the dominant vegetation type(s) and record the dominant species
x Trees Shrubs x (Grasses) Forbs Dominant species Oak species and mix deciduous
INSTREAM FEATURES
Measured reach length ___100__m Canopy Cover Estimated stream width _35__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area __350_m2
Estimated stream depth _008_m Proportion of Reach Represented by Stream High water mark _15_m Morphology Types Surface velocity (at thalweg) _05__msec x Riffle__75___ x Run__20___ x Pool__5___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___00__ m2 Density of LWD _-_ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __15_____ x Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present Algae
WATER QUALITY Temperature __19___degC Water Odors x NormalNone Sewage Specific Conductance - Petroleum Chemical Fishy Other___________________ Dissolved Oxygen - Water Surface Oils Slick Sheen Globs Flecks pH __-_ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used _____NA________ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic x None Relict shells Other______None_____ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 8 Detritus sticks wood coarse plant materials
0 Boulder gt256 mm (10rsquorsquo) 0
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 51 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 40 Sand 006-2 mm (gritty) 1 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University
SITE CHARACTERIZATION FIELD DATA SHEET FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Package Plant STATION HLR-2 STREAM CLASS LAT N 39 129580 (upstream) LONG W 078 04900 (upstream) LAT N 39 129581 (downstream) LONG W 078 04822 (downstream) FORM COMPILED BY JW Pangle RIVER BASIN Potomac River INVESTIGATORS JPangle JTovar MDahl B Millholland SRobertson W Bousquet
DATE__19 May 2008_ TIME__1240__ PM
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature _____degC showers (intermittent) Comments __Sunny Day_____________
_5__ cloud cover _0__ ______________________________
x clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x (Significant springs andor seeps) x (Valley) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS
e
f
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence FieldPasture Industrial Obvious sources Agricultural Other ____________ __Upstream Construction and Stream bank Scour Residential __________________________________ Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left Deciduous forest with thick herb and shrub layers bank= vegetation and rocks Right Deciduous forest with thick herb and shrub layers bank=vegetation soil and rocks Indicate the dominant vegetation type(s) and record the dominant species
x Trees x Shrubs Grasses Forbs Dominant species Black Walnut White Ash Hackberry and American Sycamore
INSTREAM FEATURES
Measured reach length 100 m Canopy Cover Estimated stream width _35__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area __350_m2
Estimated stream depth __15__m Proportion of Reach Represented by Stream High water mark __22___m Morphology Types Surface velocity (at thalweg) _035__msec x Riffle__30___ x Run__30___ x Pool__40___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___10____ m2 Density of LWD _______ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __30___ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present__________________________________________________________
WATER QUALITY Temperature __18___degC Water Odors x NormalNone Sewage Specific Conductance __-____ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ____-____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used _____NA____ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other___None_____ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach SubstrateType Characteristic
Composition in Sampling Reach
Bedrock 3 Detritus sticks wood coarse plant materials
2 Boulder gt256 mm (10rsquorsquo) 5
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 70 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 22 Sand 006-2 mm (gritty) 0 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiattrsquos Run LOCATION Rt 664 Bridge STATION HLR-3 STREAM CLASS LAT N 39 12934 (upstream) LONG W 078 05019 (upstream) LAT N 39 12958 (downstream) LONG W 078 04958 (downstream) FORM COMPILED BY Sean Robertson RIVER BASIN Potomac River INVESTIGATORS SRobertson BMillholland WBousquet MDahl JPangle
DATE 28 May 2008 TIME 1127 AM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality 2008
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature __14___degC showers (intermittent) x Trace Comments _______________________
_65 x cloud cover ___ ______________________________
x clearsunny __________________________________
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x (Significant springs andor seeps) x (Valley) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS
g
h
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence Some evidence x FieldPasture Industrial x Obvious sources Agricultural x Other Old Package Plant Downstream x Residential Bridge and Road Power line and Pump station Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left 70 field 30 shrubs and deciduous trees Bank=Soil and vegetation Right Maturing deciduous forest steep slope Bank=Shale Bedrock Indicate the dominant vegetation type(s) and record the dominant species
x Trees x Shrubs x Grasses Forbs Dominant species Sycamore and Red Oaks
INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width __51___m Fully open x Partly open Partly shaded Fully shaded Sampling reach area _510_m2
Estimated stream depth __135___m Proportion of Reach Represented by Stream High water mark __-___m Morphology Types Surface velocity (at thalweg) __20___msec x Riffle_40_ x Run_50_ x Pool 10 Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___0____ m2 Density of LWD ___-____ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation ___50____ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present Brown Algae
WATER QUALITY Temperature __17___degC Water Odors x NormalNone Sewage Specific Conductance ___-___ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ___-_____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used ____NA_______ Clear x Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other____None____ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 15 Detritus sticks wood coarse plant materials
0 Boulder gt256 mm (10rsquorsquo) 15
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 62 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 8 Sand 006-2 mm (gritty) 0 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Unnamed Tributary STATION HLR-4 STREAM CLASS LAT N 39 13035 (upstream) LONG W 078 05129 (upstream) LAT N 39 13000 (downstream) LONG W 078 05086 (downstream) FORM COMPILED BY Marie Dahl RIVER BASIN Potomac River INVESTIGATORS M Dahl HKepple SRobertson AFisher
DATE 31 May 2008 TIME 5 PM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality 2008
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) YES x NO
rain (steady rain) Air Temperature __22___degC showers (intermittent) Comments _______________________
_0__ cloud cover _0__ ______________________________
x clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area __-___ km2 mi2 Montane Significant springs andor seeps x Valley Marsh or swamp Montane amp Valley Mixture of origins Other ___________________
COMMENTS Snowden Bridge development (upstream) and sewer line
i
j
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence x FieldPasture Industrial Obvious sources Agricultural Other ____________ Construction upstream and adjacent x Residential Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left Forested with rocky banks field across silt fence and 2m tree strip Right Forested with some rocky banks and forest through the highway Indicate the dominant vegetation type(s) and record the dominant species
x Trees x Shrubs x Grasses Forbs Dominant species White Ash and Hackberry
INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width ___38__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area __380__m2
Estimated stream depth __12__cm Proportion of Reach Represented by Stream High water mark __2___m Morphology Types Surface velocity (at thalweg) __46___msec x Riffle__25___ x Run__70___ x Pool__5___ Channelized Yes x No Dam Present x Yes No
LARGE WOODY DEBRIS
LWD _______ m2 Density of LWD _______ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation ___NA____ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present___Algae___
WATER QUALITY Temperature __16___degC Water Odors x NormalNone Sewage Specific Conductance __-____ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ___-_____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used ____NA_____ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells x Other___Shale_______ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 0 Detritus sticks wood coarse plant materials
10 Boulder gt256 mm (10rsquorsquo) 5
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 45 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 30 Sand 006-2 mm (gritty) 0 Marl grey shell
fragments 0
Silt 0004-006 mm 20 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Lick Run STATION HLR-5 STREAM CLASS LAT N 39 127517 (upstream) LONG W 078 05685(upstream) LAT N 39 12784 (downstream) LONG W 078 05631 (downstream) FORM COMPILED BY B Millholland RIVER BASIN Potomac River INVESTIGATORS B Millholland JTovar SRobertson MDahl WBousquet JPangle
DATE 29 May 2008 TIME 1040 AM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project
k
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature __15___degC showers (intermittent) Comments _______________________
_5_ X cloud cover _0__ ______________________________
x clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane Significant springs andor seeps x Valley Marsh or swamp Montane amp Valley Mixture of origins Other ___________________
COMMENTS Lick Run appears to be intermittent and may be dry in Fall 2008
l
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence x FieldPasture Industrial Obvious sources Agricultural Other ____________ Adjacent logging stream bank scour Residential Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left 85 young deciduous forest 15 logged Bank=Soil vegetation and cobbles Right Young deciduous forest on 30 slope Bank= Soil vegetation and cobble Indicate the dominant vegetation type(s) and record the dominant species
x Trees Shrubs Grasses Forbs Dominant species Mixed Deciduous INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width __11__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area 110 m2
Estimated stream depth _009__m Proportion of Reach Represented by Stream High water mark __96___m Morphology Types Surface velocity (at thalweg) __01___msec x Riffle__30___ x Run__5___ x Pool__65___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___0____ m2 Density of LWD ___-____ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __70___ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present__________________________________________________________
WATER QUALITY Temperature __14___degC Water Odors x NormalNone Sewage Specific Conductance __-__ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ___-___ Water Surface Oils Slick Sheen Globs Flecks pH __-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used _____NA______ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other_____None_____
SEDIMENT AND SUBSTRATE
Odors Deposits Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other________________ x Other Slightly Salty Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 0 Detritus sticks wood coarse plant materials
5 Boulder gt256 mm (10rsquorsquo) 0
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 75 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 23 Sand 006-2 mm (gritty) 2 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION McCann Farm STATION HLR-6 STREAM CLASS LAT N 39 13076 (upstream) LONG W 078 06539 (upstream) LAT N 39 13063 (downstream) LONG W 078 06473 (downstream) FORM COMPILED BY MDahl RIVER BASIN Potomac River INVESTIGATORS MDahl JPangle SRobertson W Bousqet BMillholland JTovar H Kepple
DATE 30 May 2008 TIME 1050 AM (start)
PROJECT NAME
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) YES x NO
rain (steady rain) Air Temperature __18___degC showers (intermittent) Comments _______________________
_85_x cloud cover _10__ ______________________________
clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x (Significant springs andor seeps) x ( Valley) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS Multi-habitat sampling with D-frame nets and seine
m
n
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial x No evidence Some evidence x FieldPasture Industrial Obvious sources x Agricultural Other ____________ Minor stream bank scour Residential Local Watershed Erosion x None Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left 35 bank grasses scattered trees and some bushes Right High bank with grasses and scattered trees Indicate the dominant vegetation type(s) and record the dominant species
x Trees Shrubs Grasses Forbs Dominant species Black Walnut and Festuca elatior
INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width __2___m Fully open Partly open x Partly shaded Fully shaded Sampling reach area __200_m2
Estimated stream depth __03_m Proportion of Reach Represented by Stream High water mark __1___m Morphology Types Surface velocity (at thalweg) __166_msec x Riffle__0___ x Run__55___ x Pool__45___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___2____ m2 Density of LWD ___-____ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __20_____ x Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present ____Glyceria (grass)sp__________
WATER QUALITY Temperature __16___degC Water Odors x NormalNone Sewage Specific Conductance ___-___ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ____-____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used ______NA___ Clear x Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other__NONE__ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 0 Detritus sticks wood coarse plant materials
5 Boulder gt256 mm (10rsquorsquo) 0
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 27 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 38 Sand 006-2 mm (gritty) 5 Marl grey shell
fragments 0 (lt1)
Silt 0004-006 mm 25 Clay lt 0004 mm (slick) 5 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Appendix B
WATER QUALITY ANALYSIS THROUGH THE VIRGINIA
STREAM CONDITION INDEX (VSCI)
The Virginia Stream Condition Index (VSCI) relies on eight metrics to categorize stream health According to Burton and Gerritsen (2003 p 1-1) this index ldquohellip can be used as a primary indicator of ecosystem health and can identify impairment with respect to the reference (or natural) conditionrdquo These metrics are numeric measures of a biological communityrsquos species composition diversity ecological niches or tolerance to pollution
Table 13 on the next page describes each VSCI metric lists its formula and provides the best standard value (BSV) for that metric Based on a 100-point scale the BSV is the score that reflects optimum conditions for aquatic life ie the best water quality value possible (DEQ 2006b pp 8 33) The table also explains whether the score increases or decreases with environmental stress
As Table 13 indicates each of the eight VSCI metrics is calculated individually Each formula provides a unitless score based on a 100-point scale with 0 as the worst possible score and 100 as the best Any metric that provides a result above 100 is changed to 100 in order for the index to work properly After the eight metrics are calculated they are summed and averaged to provide a final VSCI score on a 100-point scale
Based on statistical analysis of Virginiarsquos streams and their BMI communities the DEQ (2006b p 8) currently uses the following tiers to interpret VSCI scores
VSCI Scores Aquatic Life Use
(ALI) Tiers
lt 42 severely stressed
42-55 moderately stressed
55-63 unknownfair (gray zone)
63-73 good
gt73 excellent
Section 2 of this report presents the results of calculations for the six BMI sampling sites the research team studied in the Hiatt-Lick Run subwatershed in 2008
o
p
1 Total taxa the number of distinct taxa in the sample measures the overall diversity of the macroinvertebrate assemblage The best standard value (BSV) is 189 Score = 100 x (X189) Total taxa decreases with stress 2 EPT taxa the sum of the distinct taxa in the orders Ephemeroptera (mayflies) Plecoptera (stoneflies) and Trichoptera (caddisflies) These orders are known to be sensitive to pollution The best standard value (BSV) is 117 Score = 100 x (X117) EPT taxa decreases with stress 3 Percent Ephemeroptera the percent abundance of individuals in the sample that belong to the order Ephemeroptera (mayflies) The best standard value (BSV) is 477 Score = 100 x (X477) Percent Ephemeroptera decreases with stress 4 Percent Plecoptera + Trichoptera less Hydropsychidae the percent abundance of individuals in the sample that belong to the orders Plecoptera (stoneflies) and Trichoptera (caddisflies) excluding the pollution-tolerant caddisfly family Hydropsychidae The best standard value (BSV) is 562 Score = 100 x (X562) Percent Plecoptera + Trichoptera less Hydropsychidae decreases with stress 5 Percent Scrapers the percent abundance of individuals in the sample whose primary functional mechanism for feeding is grazing on the substrate or periphyton (ie attached algae and associated material) The best standard value (BSV) is 467 Score = 100 x (X467) Percent scrapers decreases with stress 6 Percent Chironomidae the percent abundance of individuals in the sample that are Chironomidae (non-biting midge) larvae and pupae The best standard value (BSV) is 16 Score = 100 x [(100-X)(100-16)] Percent Chironomidae increases with stress 7 Percent Top Two Dominant the percent abundance of the two most frequent taxa present in the sample The best standard value (BSV) is 328 Score = 100 x [(100-X)(100-328)] The Percent Top 2 Dominant increases with stress 8 Hilsenhoff Biotic Index (HBI) an abundance-weighted average pollution tolerance of all the individuals within a sample keyed to the family level Tolerance ratings range from 1 (highly intolerant) to 10 (highly tolerant) The best standard value (BSV) is 29 Score = 100 x [(10-X)(10-29)] The Hilsenhoff Biotic Index (HBI) increases with stress ______________________________________________________________________________ Table 13 Description of Benthic Macroinvertebrate VSCI Metrics Includes responses (increases decreases) of each VSCI metric to degraded water quality conditions Adapted from Burton and Gerritsen (2003 pp 3-15 3-16 6-2) and DEQ (2008)
q
Appendix C
LABORATORY BENCH SHEETS
Shenandoah Universityrsquos Environmental Studies Department developed the Benthic Macroinvertebrate Laboratory Bench Sheet and VSCI Worksheet to record BMI specimen identifications and their VSCI classifications for each sample
This form is based in part on bench sheets developed by the EPA and Virginia DEQ for recording specimen identifications Additional columns on Shenandoah Universityrsquos forms for relative numbers and VSCI classifications make the calculation and verification of VSCI scores easier
Note the column for taxonomistrsquos initials Specimens were identified by students in Shenandoah Universityrsquos research group and then verified or where necessary changed by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality This external review helps assure the validity of the study Originals of these bench sheets with handwritten taxonomistsrsquo initials will be kept on file at Shenandoah Universityrsquos Environmental Studies Department for five years after the completion of this study
r
s
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-1 STATION NAME Mouth LAT N 39deg 12749rsquo (upstream) LONG W 078deg 04209rsquo (upstream) LAT N 39deg 12729rsquo (downstream) LONG W 078deg 04148rsquo (downstream) FORM COMPILED BY Jeremy Tovar W S Bousquet RIVER BASIN Potomac River INVESTIGATORS Marie Dahl Brandon Millholand J W Pangle Sean Robertson Jeremy Tovar Woodward Bousquet Ben Bousquet
Collection date 21 May 2008 Sheet completion date 12 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Hirudinea 3 33 JT BVW PR 8 SP Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Coenagrionidae 2 22 BVW PR 9 CR Ephemeroptera (mayflies) Caenidae 1 11 JT BVW CG 6 SP Ephemerllidae 4 44 JT BVW CG 2 CR Heptageniidae 5 55 JT BVW SC 3 CG Isonychiidae 1 11 BVW CF 3 CG Plecoptera (stoneflies) Perlidae 14 154 JT BVW PR 2 CR Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Corydalidae 1 11 JT BVW PR 5 CR Coleoptera (beetles) Elmidae 24 264 I JT BVW SC 4 CG Dytiscidae 4 44 JT BVW PR 6 GN Psephenidae 3 33 JT BVW SC 4 CG Diptera (true flies) Tipulidae 3 33 JT BVW SH 4 BU Chironomidae 26 286 JT BVW CG 6 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
t
Other Total Individuals 91 1001 Total Taxa 13
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
u
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-1 STATION NAME Mouth LAT N 39deg 12749rsquo (upstream) LONG W 078deg 04209rsquo (upstream) LAT N 39deg 12729rsquo (downstream) LONG W 078deg 04148rsquo (downstream) FORM COMPILED BY Jeremy Tovar W S Bousquet RIVER BASIN Potomac River INVESTIGATORS Brandon Millholand JW Pangle Jeremy Tovar Heather Kepple
Collection date 10 October 2008 Sheet completion date 12 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Oligochaeta 3 35 JT BVW CG 8 BU Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Coenagrionidae 2 23 JT BVW PR 9 CR Ephemeroptera (mayflies) Heptageniidae 12 140 JT BVW SC 3 CG Isonychiidae 2 23 BVW CF 3 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Hydropsychidae 16 186 JT BVW CF 6 CG Philopotamidae 31 360 JT BVW CF 2 CG Hemiptera (true bugs) (Veliidae) (5) - JT BVW ( ) Megaloptera (dobsonflies etc) Corydalidae 10 116 JT BVW PR 5 CR Coleoptera (beetles) Dytiscidae 1 12 JT BVW PR 6 GN Psephenidae 7 81 JT BVW SC 4 CG Diptera (true flies) Tipulidae 2 23 BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
v
Other Total Individuals 86
(91) 999
Total Taxa 10 (11)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009 Veliidae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
w
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-2 STATION NAME Package Plant LAT N 39deg 12958rsquo (upstream) LONG W 078deg 04900 (upstream) LAT N 39deg 12958rsquo (downstream) LONG W 078deg 04822 (downstream) FORM COMPILED BY JW Pangle W Bousquet RIVER BASIN Potomac River INVESTIGATORS JW Pangle S Robertson J Tovar M Dahl B Millholland W Bousquet
Collection date 29 May 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Plecoptera (stoneflies) Perlidae 11 136 I JP BVW PR 1 CR Trichoptera (caddisflies) Hydropsychidae 10 123 I JP BVW CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 14 173 I JP BVW SC 4 CG Diptera (true flies) Chironomidae 32 395 I JP BVW CG 6 BU Simuliidae 14 173 I JP BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
x
Other Total Individuals 81 1000 Total Taxa 5
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-2 STATION NAME Package Plant LAT N 39deg 12958rsquo (upstream) LONG W 078deg 04900 (upstream) LAT N 39deg 12958rsquo (downstream) LONG W 078deg 04822 (downstream) FORM COMPILED BY JW Pangle W Bousquet RIVER BASIN Potomac River INVESTIGATORS JW Pangle Brandon Millholland Jeremy Tovar Heather Kepple
Collection date 10 October 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Heptageniidae 40 392 I WBJP SC 3 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Hydropsychidae 7 69 I JP WB BVW CF 6 CG Philopotamidae 28 275 I JP WB BVW CF 2 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Corydalidae 6 59 I JP WB BVW PR 5 CR Coleoptera (beetles) Psephenidae 7 69 I JP WB BVW SC 4 CG Elmidae 8 78 I JP WB BVW SC 4 CG Diptera (true flies) Tipulidae 6 59 I JP WB BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
y
z
Other Total Individuals 102 1001 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-3 STATION NAME Rt 664 Bridge LAT N 39deg 12934rsquo (upstream) LONG W 078deg 05019rsquo (upstream) LAT N 39deg 12951rsquo (downstream) LONG W 078deg 04958rsquo (downstream) FORM COMPILED BY S Robertson W Bousquet RIVER BASIN Potomac River INVESTIGATORS SRobertson BMillholland WBousquet MDahl JPangle
Collection date 28 May 2008 Sheet completion date 11 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Leptophlebiidae 1 15 I SGR BVW CG 2 CR Baetidae 1 15 I BVW CG 5 CG Plecoptera (stoneflies) Perlidae 3 46 I SGR BVW PR 1 CR Nemouridae 1 15 I BVW SH 3 CR Trichoptera (caddisflies) Hydropsychidae 5 77 I SGR BVW CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 27 415 I SGR BVW SC 4 CG Psephenidae 2 31 A SGR BVW SC 4 CG Diptera (true flies) Simuliidae 5 77 I SGR BVW CF 6 CG Chironomidae 20 308 I SGR BVW CG 6 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
aa
bb
Other Total Individuals 65 999 Total Taxa 9
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-3 STATION NAME Rt 664 Bridge LAT N 39deg 12934rsquo (upstream) LONG W 078deg 05019rsquo (upstream) LAT N 39deg 12951rsquo (downstream) LONG W 078deg 04958rsquo (downstream) FORM COMPILED BY S Robertson RIVER BASIN Potomac River INVESTIGATORS S Robertson M Dahl A Fisher H Kepple
Collection date 10 Oct 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Heptageniidae 2 20 I SGR SC 3 CG Isonychiidae 2 20 I BVW CF 2 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Philopotamidae 36 367 I SGR CF 2 CG Hydropsychidae 47 480 I SGR CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 2 20 A SGR SC 4 CG Psephenidae 8 82 A SGR SC 4 CG Diptera (true flies) Tabanidae 1 10 I SGR PR 7 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
cc
dd
Other Total Individuals 98 999 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-4 STATION NAME Unnamed Tributary LAT (upstream) N 39deg 13035rsquo LONG (upstream) W 078deg 05129rsquo LAT (downstream) N 39deg 13000rsquo LONG (downstream) W 078deg 5086rsquo FORM COMPILED BY A Fisher M Dahl rev W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson A Fisher H Kepple
Collection date 31 May 2008 Sheet completion date rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Flatworms (Turbellaria) Worms (Oligochaeta) Naididae 1 14 - BVW CG 8 BU Leeches (Hirudinea) Snails (Gastropoda) Clams (Bivalvia) Isopods (Isopoda) Asellidae 3 42 A AF BVW CG 8 SP Scuds (Amphipoda) Crayfish Shrimp (Decapoda) Water Mites (Hydracarina) Dragonflies (Odonata Anisoptera) Damselflies (Odonata Zygoptera) Mayflies (Ephemeroptera) Stoneflies (Plecoptera) Perlidae 2 28 I AF BVW PR 1 CR Caddisflies (Trichoptera) True Bugs (Hemiptera) Corixidae 1 14 I AF BVW MP 9 DV Dobsonflies (Megaloptera) Beetles (Coleoptera) Dytiscidae 1 14 I AF BVW PR 6 GN True Flies (Diptera) Chironomidae 61 847 I AF BVW CG 6 BU Simuliidae 3 42 I AF BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
ee
ff
Other Total Individuals 72 1001 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-4 STATION NAME Unnamed Tributary LAT (upstream) N 39deg 13035rsquo LONG (upstream) W 078deg 05129rsquo LAT (downstream) N 39deg 13000rsquo LONG (downstream) W 078deg 5086rsquo FORM COMPILED BY Andrew G M Fisher W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson A Fisher H Kepple
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Flatworms (Turbellaria) Worms (Oligochaeta) Lumbriculidae 2 32 A AF BVW CG 8 BU Leeches (Hirudinea) Snails (Gastropoda) Lymnaeidae 1 16 A BVW CG 9 GN Clams (Bivalvia) Isopods (Isopoda) Scuds (Amphipoda) Crayfish Shrimp (Decapoda) Cambaridae 1 16 A AF BVW GN 5 GN Water Mites (Hydracarina) Dragonflies (Odonata Anisoptera) Damselflies (Odonata Zygoptera) Calopterygidae 4 63 I BVW PR 7 CL Mayflies (Ephemeroptera) Tricorythidae 1 16 I BVW CG 5 SP Stoneflies (Plecoptera) Caddisflies (Trichoptera) Phryganeidae 4 63 I AF BVW SH 4 CR Hydropsychidae 6 95 I AF BVW CF 6 CG I True Bugs (Hemiptera) Corixidae 8 127 I AF BVW MP 9 DV (Veliidae ) (7) - I AF BVW ( ) Dobsonflies (Megaloptera) Sialidae 3 48 I AF BVW PR 7 SP Corydalidae 2 32 I AF BVW PR 5 CR Beetles (Coleoptera) Haliplidae 1 16 A AF BVW MP 8 GN Dysticidae 7 111 I AF BVW PR 6 GN Hydrophilidae 1 16 A AF BVW PR 8 GN Elmidae 3 48 I BVW SC 4 CG True Flies (Diptera) Chironomidae 8 127 I AF BVW CG 6 BU Simuliidae 7 111 I AF BVW CF 6 CG Tipulidae 4 63 I AF BVW SH 4 GU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
gg
hh
Other Total Individuals 63
(70) 1000
Total Taxa 17 (18)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Veliidae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-5 STATION NAME Lick Run LAT N 39deg 12752rsquo (upstream) LONG W 078deg 05685rsquo (upstream) LAT N 39deg 12784rsquo (downstream) LONG W 078deg 05631rsquo (downstream) FORM COMPILED BY Brandon Millholland W Bousquet RIVER BASIN Potomac River INVESTIGATORS J Pangle S Robertson W Bousquet JTovar M Dahl
Collection date 29 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Gomphidae 1 15 I BVW PR 3 BU Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Leptophlebiidae 11 167 A LBM BVW CG 2 CR Plecoptera (stoneflies) Perlidae 10 152 A LBM BVW PR 1 CR Perlodiae 1 15 A LBM BVW PR 2 CR Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 5 76 A LBM BVW SC 4 CG Elmidae 2 30 I LBM BVW SC 4 CG Diptera (true flies) (Culicidae) (3) - P LMB BVW ( ) Chironomidae 23 348 I LBM BVW CG 6 BU Simuliidae 13 197 I LBM BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
ii
jj
Other Total Individuals 66
(69) 1000
Total Taxa 7 (8)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Culicidae (Diptera) pupae not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl J Pangle S Robertson W Bousquet B Millholland J Tovar H Kepple
Collection date 30 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 1 12 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Asellidae 114 965 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Plecoptera (stoneflies) Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 2 23 A MRD BVW SC 4 CG Diptera (true flies)
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
kk
ll
Other Total Individuals 117 1000 Total Taxa 3
Notes Subsample taken 29 October 2009 All taxonomic determinations by MRD were confirmed by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson K McCann A Fisher H Kepple A Barrett
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 2 17 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Sphaeriidae 2 17 I MRD BVW CF 8 BU Isopoda (isopods) Asellidae 99 853 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Calopterygidae 2 17 A MRD BVW PR 7 CL Ephemeroptera (mayflies) Leptophlebiidae 2 17 I MRD BVW CG 2 CR Heptageniidae 4 34 A MRD BVW SC 3 CG Plecoptera (stoneflies) Perlidae 1 09 A MRD BVW PR 1 CR Trichoptera (caddisflies) Hemiptera (true bugs) (Gerridae) (1) - A MRD BVW ( ) Corixidae 1 09 A MRD BVW PR 5 CR Megaloptera (dobsonflies etc) Coleoptera (beetles) Dryopidae 1 09 A MRD BVW SC 5 CG Diptera (true flies) Tipulidae 2 17 I BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
mm
nn
Other Total Individuals 116
(117) 999
Total Taxa 10 (11)
Notes Subsample taken 6 November 2008 Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009 Gerridae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
- Disclaimer ii
- Title Page iii
- Acknowledgements v
- Table of Contents vii
- SECTION 2 RESULTS DATA TABLES 7
- OVERVIEW AND FINDINGS
- Introduction
- RESULTS DATA TABLES
- REFERENCES
- FIELD DATA SHEETS
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- SubstrateType
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- WATER QUALITY ANALYSIS
- THROUGH THE VIRGINIA
- STREAM CONDITION INDEX (VSCI)
- LABORATORY BENCH SHEETS
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
ACKNOWLEDGEMENTS
Many individuals and organizations provided time support and expertise for this research
study Although we cannot list everyone by name we would like to express our sincere gratitude to all who made the project possible including Jim Lawrence ndash Coordinator Opequon Targeted Watershed Project Property owners along Hiatt Run and Lick Run
Mike Hoffman Leslie Johns The McCann-Slaughter Family Ervin and Barbara Simons Tonie Wallace-Aitken and Greg Aitken Historic Jordan Springs Event and Cultural Centre
Billy van Wart ndash Regional Aquatic Biologist Virginia Department of Environmental Quality Heather Kepple ndash Technician Opequon Targeted Watershed Project Field Assistance Andrew Barrett
Ben Bousquet Katherine McCann
Financial Support
Opequon Creek Targeted Watershed Grant Dr and Mrs Charles Leeper An anonymous family foundation Shenandoah University
v
vi
TABLE OF CONTENTS page
Disclaimer ii Title Page iii Acknowledgements v Table of Contents vii SECTION 1 OVERVIEW AND FINDINGS 1 Introduction 1 Methods and Analysis 3 Findings 3 Future Research 5 SECTION 2 RESULTS DATA TABLES 7 Site HLR-1 Mouth 7 Site HLR-2 Package Plant 8 Site HLR-3 Route 664 Bridge 9 Site HLR-4 Unnamed Tributary 10 Site HLR-5 Lick Run 11 Site HLR-6 McCann Farm 12 REFERENCES 13 APPENDICES 15 Appendix A Field Data Sheets Appendix B Water Quality Analysis through the Virginia Stream Condition Index (VSCI) Appendix C Laboratory Bench Sheets Note Photographs of the six sampling sites appear on a CD provided separately to the grant administrator
vii
viii
Section 1
OVERVIEW AND FINDINGS
Introduction
This report presents baseline water quality data for Hiatt Run and Lick Run -- termed the Hiatt-Lick Run subwatershed in this study Located in Frederick County Virginia Hiatt and Lick Runs are tributaries of Opequon Creek The Opequon in turn empties into the Potomac River and then the Chesapeake Bay The investigation was conducted by a Shenandoah University research team in 2008 Researchers used benthic macroinvertebrates (BMIs) as biological indicators of water quality This report presents an overview of the study followed by summary tables and a list of references The Appendix contains data sheets and a description of the data analysis These data will form part of the baseline water quality assessment that is being undertaken through the Opequon Creek Targeted Watershed Grant The grant administered by Jim Lawrence reflects a broad-based partnership that includes Virginia Tech Shenandoah University the Frederick-Winchester Service Authority The Opequon Watershed (TOW) citizens organization as well as federal state and local governments community groups and business interests Grant partners will use best management practices (BMPs) to reduce nutrient runoff and address other pollution problems in the Opequon Creek watershed Since 1996 Opequon Creek has appeared on the impaired waters list prepared by the Virginia Department of Environmental Quality (DEQ) The Hiatt-Lick Run subwatershed was selected for this study because it represents changes that are typical of the Opequon Creek watershed These changes include new commercial and residential developments as well as the implementation of water quality improvement practices such as tree planting in riparian zones fences to prevent livestock from entering streams and replacing outdated sewage treatment facilities such as a package plant on lower Hiatt Run
Seven students from Shenandoah Universityrsquos Environmental Studies Department conducted this study from May through December 2008 under the direction of Woodward Bousquet Professor of Environmental Studies and Biology The research team followed procedures described in the Rapid Bioassessment Protocols (RBPs) of the US Environmental Protection Agency (Barbour et al 1999) and the Virginia Stream Condition Index (VSCI) The VSCI provides an evaluation of water quality based on a streamrsquos benthic macroinvertebrate community (Burton and Gerritsen 2003 DEQ 2006b and 2008)
In consultation with Billy van Wart of the DEQ and grant administrator Jim Lawrence the Shenandoah University researchers selected six representative sampling sites in the Hiatt-Lick Run subwatershed Each site was given a name and an HLR (for Hiatt-Lick Run) code Figure 1 maps their positions while their characteristics and coordinates appear in the field data sheets (see Appendix A) These sites were established according to EPA and DEQ guidelines that generally call for a 100-meter-long stream reach with at least three riffle zones a width sufficient to accommodate a 1-meter seine and a substrate composed of least 35 cobbles
Specific locations of the sampling sites on Hiatt and Lick Runs were selected to reflect different watershed positions and changing land uses See Figure 1 Further information about each sampling site appears in the field data sheets (Appendix A)
1
Figure 1 Sampling Sites in the Hiatt-Lick Run Subwatershed of Opequon Creek Frederick County Virginia Scale 1 centimeter = 500 meters
HLR-1 (Mouth) measures water quality at the lowest point of the subwatershed as Hiatt Run flows into Opequon Creek Forests cover the immediate riparian zones on both sides of Hiatt Run This site will be used in future years by the DEQ for BMI monitoring HLR-2 (Package Plant) is situated on Hiatt Run just below the outflow pipe of an outdated package plant that formerly treated sewage from the Jordan Springs area During the study period the package plant was taken off-line and a pumping station was constructed The Frederick-Winchester Service Authority now treats this sewage at its Opequon Water Reclamation Facility HLR-3 (Route 664 Bridge) was chosen because it is located upstream from the package plant but downstream of the confluence of Hiatt Run and Lick Run HLR-4 (Unnamed Tributary) is located downstream of the Snowden Bridge residential development which has been under construction The area immediately upstream of this sampling site was being graded by heavy equipment during the May 2008 sampling time HLR-5 (Lick Run) the researchers learned dries up in late summer It had a modest but constant flow during when the May 2008 sample was taken HLR-6 (McCann Farm) represents the headwater areas of the Hiatt-Lick Run subwatershed of Opequon Creek The McCanns who graze cattle in their pastures that surround Hiatt Run here have been working with the Potomac Conservancy to implement Best Management Practices
2
(BMPs) to improve water quality These practices include fencing cattle out of the stream and spring runs and planting more trees in the riparian zone
Methods and Analysis
The research team established a sampling plan and then sampled according to methods the DEQ developed for the VSCI Using a 1 meter x 1 meter seine samples from 2m2 of substrate in riffle zones were to be collected twice at each site once in May 2008 and once in October 2008 At five of the six sites stream characteristics permitted the standard single-habitat sampling procedure At the McCann Farm (Site HLR-6) researchers needed to use multi-habitat sampling See Barbour et al (1999 pp 7-3 ff) for further descriptions of these two methods
All six sites were sampled in May 2008 Low-water conditions in October 2008 prevented the Shenandoah University group from collecting BMIs at Lick Run (HLR-5) that month but the other five sites were sampled successfully As a result the research team obtained eleven samples for analysis in this study Samples were preserved in ethanol for subsequent identification
DEQ procedures require subsampling when the number of BMIs in a given sample exceeds 100 All eleven samples in this study met that criterion Consequently researchers used a Caton tray to produce random subsamples of 90 to 110 specimens from each sample During the analysis that followed some specimens in the subsamples were rejected either because they turned out to be terrestrial species or because they were in the wrong life stage (eg pupae) In most cases the researchers identified specimens to the family level Primary taxonomic references were A Guide to Common Freshwater Invertebrates of North America (Voshell 2002) Fresh-Water Invertebrates of the United States (Pennak 1953) and An Introduction to the Aquatic Insects of North America (Merritt and Cummins 1996) DEQ Regional Aquatic Biologist Billy van Wart reviewed verified and where necessary corrected the identifications
The investigators then used the Virginia Stream Condition Index (VSCI) to produce water quality scores based on the BMIs collected A VSCI score is based on eight different measurement formulas called metrics Each metric is a numeric measure of a biological communityrsquos species composition diversity ecological niches or tolerance to pollution When combined into a single multimetric VSCI score the response of the benthic community to water conditions can be evaluated For further information and worksheets see Appendices B and C respectively
Findings
Table 1 presents the VSCI scores for the eleven samples collected in the Hiatt-Lick Run subwatershed in 2008 Since a sample year consists of two samples the mean scores for each site for the entire year are also included As the table shows
bull None of the 11 samples was rated excellent bull Only 1 of the 11 samples was rated as good bull Only 2 of the 11 samples were rated fair (ie in the ldquogrey zonerdquo between impaired and
unimpaired) bull The remaining 8 samples were in either moderately stressed or severely stressed
condition
3
Site Name and Code Sampling
Date of
TaxaVCSI Score
Water Quality Rating
Mouth (HLR-1) 21 May 2008 13 571 Fair (Gray Zone)
Mouth (HLR-1) 10 Oct 2008 10 608 Fair (Gray Zone)
site average for 2008 590 Fair (Gray Zone)
Package Plant (HLR-2) 29 May 2008 5 370 Severely Stressed
Package Plant (HLR-2) 10 Oct 2008 7 674 Good
site average for 2008 522 Moderately Stressed
Route 664 Bridge (HLR-3) 28 May 2008 9 485 Moderately Stressed
Route 664 Bridge (HLR-3) 10 Oct 2008 7 469 Moderately Stressed
site average for 2008 477 Moderately Stressed
Unnamed Tributary (HLR-4) 31 May 2008 7 173 Severely Stressed
Unnamed Tributary (HLR-4) 10 Oct 2008 17 476 Moderately Stressed
site average for 2008 325 Severely Stressed
Upper Lick Run (HLR-5) 29 May 2008 7 455 Moderately Stressed
site average for 2008 455 Moderately Stressed
McCann Farm (HLR-6) 30 May 2008 3 190 Severely Stressed
McCann Farm (HLR-6) 10 Oct 2008 10 316 Severely Stressed
site average for 2008 253 Severely Stressed
Summary 6 sites 11 samples
36 BMI taxa
VSCI = Virginia Stream Condition Index see Burton and Gerritsen (2003) and Virginia Department of Environmental Quality (2006b and 2008) Table 1 Summary Table of Virginia Stream Condition Index (VSCI) Scores and Water Quality Ratings for Sites in the Hiatt-Lick Run Subwatershed of Opequon Creek Frederick County Virginia 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
4
Improvements in water quality ratings at three sites between May and October suggest that BMPs and other changes may already be having some positive effects on water quality At the Package Plant (Site HLR-2) the VSCI score rose dramatically from 370 (severely stressed) to 674 (good) The small-scale sewage treatment plant as noted above was taken off-line during this period Similarly water quality at the Unnamed Tributary (HLR-4) increased from 173 (severely stressed) to 476 (moderately stressed) Adjacent construction was completed over the summer and grass was planted on the property Management practices on the McCann Farm (HLR-6) may be responsible for improving conditions for aquatic life in Hiatt Runrsquos headwaters Even though the water quality was still rated as severely stressed in October the VSCI score rose from 190 to 316 at this sampling site Further studies are needed to determine whether the changes noted in 2008 persist into the future In 2003 the DEQ began a Total Maximum Daily Load (TMDL) process for Opequon Creek as required by the Water Quality Act of 1987 The TMDL approach establishes a ldquopollution budgetrdquo for each impaired stream for each type of impairment and for each responsible pollutant In conjunction with the DEQ and the Virginia Department of Conservation and Recreation (DCR) Virginia Polytechnic Institutersquos Department of Biosystems Engineering developed a TMDL implementation plan (Opequon Creek IP Steering Committee et al 2006) Monitoring the BMI communities of Opequon Creek and its tributaries is essential to evaluating the progress of the proposed water quality improvement projects
Future Research The relatively small Hiatt-Lick Run subwatershed provides several case studies that can help assess the effectiveness of various water quality management practices not only in Opequon Creek but also in other watersheds in the Chesapeake Bay region and beyond In supporting that goal this study makes two principal contributions 1 It provides baseline data to measure the improvement or degradation of the Hiatt-Lick Run section of the Opequon Creek watershed and 2 It establishes a monitoring framework for the long-term biological monitoring of water quality and the evaluation of best management practices (BMPs) This framework consists of
bull Sampling Sites ndash six stream reaches in the Hiatt-Lick Run subwatershed selected as sampling sites according to EPA and DEQ specifications
bull Data Collection Protocols ndash based on the EPArsquos Rapid Bioassessment Protocols (RBPs)
(Barbour et al 1999) and the DEQrsquos Virginia Stream Index (VSCI) (Burton and Gerritsen 2003 DEQ 2006b and 2008) and
bull Analytical Procedures ndash contained in the Virginia Stream Condition Index (VSCI see
Burton and Gerritsen 2003 DEQ 2006b pp 8 and 33 and DEQ 2008 pp 29-34)
5
6
Section 2
RESULTS DATA TABLES
Site HLR-1 Mouth
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 13 189 6872 EPT taxa 5 117 4273 Ephemeroptera 121 477 2544 Plec +Tric less Hydropsych 154 562 2745 Scrapers 352 467 7536 Chironomidae 286 16 7267 Top 2 Dominant 550 328 6708 Hilsenhoff Biotic Index (HBI) 45 29 775VSCI Score 571
According to the DEQrsquos water quality standards aquatic life at this site is fair (gray zone) See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 2 VSCI Scores for Mouth (HLR-1) Site in Hiatt-Lick Run Sampled on 21 May 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 10 189 5292 EPT taxa 4 117 3423 Ephemeroptera 163 477 3424 Plec +Tric less Hydropsych 360 562 6415 Scrapers 221 467 4736 Chironomidae 00 16 10007 Top 2 Dominant 546 328 6768 Hilsenhoff Biotic Index (HBI) 39 29 859VSCI Score 608
According to the DEQrsquos water quality standards aquatic life at this site is fair (gray zone) See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 3 VSCI Scores for Mouth (HLR-1) Site in Hiatt-Lick Run Sampled 10 October 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
7
Site HLR-2 Package Plant
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 5 189 2642 EPT taxa 2 117 1713 Ephemeroptera 00 477 004 Plec +Tric less Hydropsych 110 562 1965 Scrapers 173 467 3706 Chironomidae 395 16 6157 Top 2 Dominant 568 328 6438 Hilsenhoff Biotic Index (HBI) 50 29 704VSCI Score 370
According to the DEQrsquos water quality standards aquatic life at this site is severely stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 4 VSCI Scores for Package Plant (HLR-2) Site in Hiatt-Lick Run Sampled 29 May 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 7 189 3702 EPT taxa 34 117 2563 Ephemeroptera 392 477 8214 Plec +Tric less Hydropsych 275 562 4895 Scrapers 539 467 10006 Chironomidae 00 16 10007 Top 2 Dominant 667 328 4968 Hilsenhoff Biotic Index (HBI) 32 29 958VSCI Score 674
According to the DEQrsquos water quality standards aquatic life at this site is good See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 5 VSCI Scores for Package Plant (HLR-2) Site in Hiatt-Lick Run Sampled 10 October 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
8
Site HLR-3 Route 664 Bridge
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 9 189 4762 EPT taxa 5 117 4273 Ephemeroptera 30 477 634 Plec +Tric less Hydropsych 61 562 1095 Scrapers 446 467 9556 Chironomidae 308 16 7037 Top 2 Dominant 723 328 4128 Hilsenhoff Biotic Index (HBI) 48 29 732VSCI Score 485
According to the DEQrsquos water quality standards aquatic life at this site is moderately stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 6 VSCI Scores for Route 664 Bridge (HLR-3) Site in Hiatt-Lick Run Sampled 28 May 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 7 189 3702 EPT taxa 4 117 3423 Ephemeroptera 40 477 844 Plec +Tric less Hydropsych 367 562 6535 Scrapers 122 467 2616 Chironomidae 00 16 10007 Top 2 Dominant 847 328 2288 Hilsenhoff Biotic Index (HBI) 42 29 817VSCI Score 469
According to the DEQrsquos water quality standards aquatic life at this site is moderately stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 7 VSCI Scores for Route 664 Bridge (HLR-3) Site in Hiatt-Lick Run Sampled 10 October 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
9
Site HLR-4 Unnamed Tributary
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 7 189 3702 EPT taxa 1 117 853 Ephemeroptera 00 477 004 Plec +Tric less Hydropsych 28 562 495 Scrapers 00 467 006 Chironomidae 847 16 1557 Top 2 Dominant 889 328 1658 Hilsenhoff Biotic Index (HBI) 60 29 563VSCI Score 173
According to the DEQrsquos water quality standards aquatic life at this site is severely stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 8 VSCI Scores for Unnamed Tributary (HLR-4) Site in Hiatt-Lick Run Sampled 31 May 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 17 189 8992 EPT taxa 3 117 2563 Ephemeroptera 16 477 344 Plec +Tric less Hydropsych 63 562 1125 Scrapers 48 467 1036 Chironomidae 127 16 8877 Top 2 Dominant 254 328 10008 Hilsenhoff Biotic Index (HBI) 63 29 521VSCI Score 476
According to the DEQrsquos water quality standards aquatic life at this site is moderately stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 9 VSCI Scores for Unnamed Tributary (HLR-4) Site in Hiatt-Lick Run Sampled 10 October 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
10
Site HLR-5 Lick Run
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 7 189 3702 EPT taxa 3 117 2563 Ephemeroptera 167 477 3504 Plec +Tric less Hydropsych 167 562 2975 Scrapers 106 467 2276 Chironomidae 348 16 6637 Top 2 Dominant 545 328 6778 Hilsenhoff Biotic Index (HBI) 43 29 803VSCI Score 455
According to the DEQrsquos water quality standards aquatic life at this site is moderately stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 10 VSCI Scores for Lick Run (HLR-5) Site in Hiatt-Lick Run Sampled 29 May 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
Note
Water conditions at the Lick Run (HLR-5) site were too low in October 2008 to permit a sample to be taken
11
Site HLR-6 McCann Farm
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 3 189 1592 EPT taxa 0 117 003 Ephemeroptera 00 477 004 Plec +Tric less Hydropsych 00 562 005 Scrapers 23 467 496 Chironomidae 00 16 10007 Top 2 Dominant 988 328 188 Hilsenhoff Biotic Index (HBI) 79 29 296VSCI Score 190
According to the DEQrsquos water quality standards aquatic life at this site is severely stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 11 VSCI Scores for McCann Farm (HLR-6) Site in Hiatt-Lick Run Sampled 30 May 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 10 189 5292 EPT taxa 3 117 2563 Ephemeroptera 51 477 1074 Plec +Tric less Hydropsych 09 562 165 Scrapers 43 467 926 Chironomidae 00 16 10007 Top 2 Dominant 887 328 1688 Hilsenhoff Biotic Index (HBI) 75 29 352VSCI Score 315
According to the DEQrsquos water quality standards aquatic life at this site is severely stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 12 VSCI Scores for McCann Farm (HLR-6) Site in Hiatt-Lick Run Sampled 10 October 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
12
REFERENCES
Barbour Michael T Jeroen Gerritsen Blaine D Snyder and James B Stribling 1999 Rapid bioassessment protocols for use in streams and wadeable rivers periphyton benthic macroinvertebrates and fish Second edition EPA 841-B-99-002 Washington DC US Environmental Protection Agency Office of Water Available online at httpwwwepagovOWOWmonitoringbioasspshtml Burton June and Jeroen Gerritsen 2003 A stream condition index for Virginia non-coastal streams Prepared for the US EPA Office of Science and Technology US EPA Region 3 Environmental Services Division and Virginia Department of Environmental Quality Owings Mills Maryland Tetra Tech Inc Available online at httpwwwdeqvirginiagovwatermonitoringpdf vastrmconpdf Hessler Rachel A Andrea R Martin Heidi D Ruggeri William B Seymour Jr and Woodward S Bousquet 2006 Water quality assessment using benthic macroinvertebrates A study and compilation Winchester Virginia Environmental Studies Program Shenandoah University Occasional publication no 10 Igoe Robert 2006 DEQ identifies nine impaired streams in Clarke Frederick The Winchester Star 15 July B1 Jackson Charlie 2005 Survey targets Opequon cleanup The Winchester Star 19 August B1 Johnston Elizabeth A and Woodward S Bousquet 2003 What happens when it rains A study of water quality in Abrams Creek and Town Run Winchester and Frederick County Virginia Winchester Virginia Environmental Studies Program Shenandoah University Occasional publication no7 Merritt Richard W and Kenneth W Cummins 1996 An introduction to the aquatic insects of North America Dubuque Iowa KendallHunt Publishing Company Opequon Creek IP Steering Committee Virginia Tech Department of Biological Systems Engineering and Center for TMDL and Watershed Studies University of Virginia Institute for Environmental Negotiation and West Virginia University Division of Resource Management 2006 Opequon Creek watershed TMDL implementation plan Blacksburg Virginia Virginia Tech Department of Biological Systems Engineering Available online at httpwwwtmdlbsevteduoutreachC82 Pennak Robert W 1953 Fresh-water invertebrates of the United States New York New York The Ronald Press Company Rosenburg David M and Vincent H Resh 1993 Freshwater biomonitoring and benthic macroinvertebrates New York New York Chapman amp Hall Shenandoah University Public Relations Office 2002 Data collected by SU and its partners helps identify impaired water Winchester Virginia Available online at httpwwwsuedutemp_ newscfmurlnum=32 Thorp James H and Alan P Covich 1991 Ecology and classification of North American freshwater invertebrates San Diego California Academic Press Inc
13
US Environmental Protection Agency 1997 Volunteer stream monitoring a methods manual EPA 841-B-97-003 Washington DC US Environmental Protection Agency Office of Water Van Meter Val 2003 Opequon Creek pollution limits to be established The Winchester Star 4 April B1 Virginia Department of Environmental Quality 2006a Virginia 305(b)303(d) water quality integrated report to Congress and the EPA Administrator for the period January 1 2000 to December 31 2004 Richmond Virginia Virginia Department of Environmental Quality Available online at httpwwwdeqvirginiagovwqair2006html __________ 2006b Using probabilistic data to validate the non-coastal Virginia Stream Condition Index (draft) VDEQ technical bulletin WQA2006-001 Virginia Department of Environmental Quality Available online at httpwwwloudounwatershedorgstream_assessmentreference_documents VA_DEQ_SCI_Validationpdf __________ 2008 Biological monitoring program quality assurance project plan for wadeable streams and rivers Richmond Virginia Virginia Department of Environmental Quality Update available online at httpwwwdeqstatevausbiomonitoringdocumentsBioMonQAPP_13Aug2008pdf Virginia Tech Aquatic Entomology Laboratory 2005 Taxa dictionary [Excel file] Blacksburg Virginia Virginia Tech Department of Biological Systems Engineering 2003a Opequon watershed TMDLs for benthic impairments Abrams Creek and Lower Opequon Creek Frederick and Clarke Counties Virginia Prepared for the Virginia Department of Environmental Quality and Virginia Department of Conservation and Recreation Blacksburg Virginia Available online at httpwwwdeqstate vaustmdlapptmdlsshenrvrabropebcpdf Voshell J Reese Jr 2002 A guide to common freshwater invertebrates of North America Blacksburg Virginia The McDonald amp Woodward Publishing Company
14
APPENDICES
15
16
a
Appendix A
FIELD DATA SHEETS
This appendix presents a Site Characterization Field Data Sheet for each of the six sampling reaches established on Hiatt Run and Lick Run for this study They were filled out during the sampling runs made in May 2008
These forms were adapted by Shenandoah University from forms used by the EPA and Virginia DEQ for recording characteristics of sampling sites
b
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Mouth STATION _HLR-1_____ STREAM CLASS LAT N 39 12749 (upstream) LONG W 078 04209(upstream) LAT N 39 12729 (downstream) LONG W 078 04148 (downstream) FORM COMPILED BY Jeremy Tovar RIVER BASIN Potomac River INVESTIGATORS JPangle B Millholland MDahl SRobertson W Bousquet JTovar BBousquet
DATE May 21 2008_________ TIME_250 PM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature __24___degC showers (intermittent) Comments _______________________
___ cloud cover _0_ ______________________________
X clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x ( Significant springs andor seeps) x (Valley ) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS
c
d
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence x FieldPasture Industrial Obvious sources Agricultural Other ____________ Stream scour steep slope erosion x Residential Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left Field and young thin deciduous forest 100 Bank=Grass cobble and shrub Right 90 maturing deciduous forest 45 shale slope Bank=Bedrock soil and herbs Indicate the dominant vegetation type(s) and record the dominant species
x Trees Shrubs x (Grasses) Forbs Dominant species Oak species and mix deciduous
INSTREAM FEATURES
Measured reach length ___100__m Canopy Cover Estimated stream width _35__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area __350_m2
Estimated stream depth _008_m Proportion of Reach Represented by Stream High water mark _15_m Morphology Types Surface velocity (at thalweg) _05__msec x Riffle__75___ x Run__20___ x Pool__5___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___00__ m2 Density of LWD _-_ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __15_____ x Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present Algae
WATER QUALITY Temperature __19___degC Water Odors x NormalNone Sewage Specific Conductance - Petroleum Chemical Fishy Other___________________ Dissolved Oxygen - Water Surface Oils Slick Sheen Globs Flecks pH __-_ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used _____NA________ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic x None Relict shells Other______None_____ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 8 Detritus sticks wood coarse plant materials
0 Boulder gt256 mm (10rsquorsquo) 0
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 51 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 40 Sand 006-2 mm (gritty) 1 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University
SITE CHARACTERIZATION FIELD DATA SHEET FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Package Plant STATION HLR-2 STREAM CLASS LAT N 39 129580 (upstream) LONG W 078 04900 (upstream) LAT N 39 129581 (downstream) LONG W 078 04822 (downstream) FORM COMPILED BY JW Pangle RIVER BASIN Potomac River INVESTIGATORS JPangle JTovar MDahl B Millholland SRobertson W Bousquet
DATE__19 May 2008_ TIME__1240__ PM
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature _____degC showers (intermittent) Comments __Sunny Day_____________
_5__ cloud cover _0__ ______________________________
x clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x (Significant springs andor seeps) x (Valley) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS
e
f
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence FieldPasture Industrial Obvious sources Agricultural Other ____________ __Upstream Construction and Stream bank Scour Residential __________________________________ Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left Deciduous forest with thick herb and shrub layers bank= vegetation and rocks Right Deciduous forest with thick herb and shrub layers bank=vegetation soil and rocks Indicate the dominant vegetation type(s) and record the dominant species
x Trees x Shrubs Grasses Forbs Dominant species Black Walnut White Ash Hackberry and American Sycamore
INSTREAM FEATURES
Measured reach length 100 m Canopy Cover Estimated stream width _35__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area __350_m2
Estimated stream depth __15__m Proportion of Reach Represented by Stream High water mark __22___m Morphology Types Surface velocity (at thalweg) _035__msec x Riffle__30___ x Run__30___ x Pool__40___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___10____ m2 Density of LWD _______ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __30___ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present__________________________________________________________
WATER QUALITY Temperature __18___degC Water Odors x NormalNone Sewage Specific Conductance __-____ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ____-____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used _____NA____ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other___None_____ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach SubstrateType Characteristic
Composition in Sampling Reach
Bedrock 3 Detritus sticks wood coarse plant materials
2 Boulder gt256 mm (10rsquorsquo) 5
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 70 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 22 Sand 006-2 mm (gritty) 0 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiattrsquos Run LOCATION Rt 664 Bridge STATION HLR-3 STREAM CLASS LAT N 39 12934 (upstream) LONG W 078 05019 (upstream) LAT N 39 12958 (downstream) LONG W 078 04958 (downstream) FORM COMPILED BY Sean Robertson RIVER BASIN Potomac River INVESTIGATORS SRobertson BMillholland WBousquet MDahl JPangle
DATE 28 May 2008 TIME 1127 AM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality 2008
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature __14___degC showers (intermittent) x Trace Comments _______________________
_65 x cloud cover ___ ______________________________
x clearsunny __________________________________
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x (Significant springs andor seeps) x (Valley) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS
g
h
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence Some evidence x FieldPasture Industrial x Obvious sources Agricultural x Other Old Package Plant Downstream x Residential Bridge and Road Power line and Pump station Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left 70 field 30 shrubs and deciduous trees Bank=Soil and vegetation Right Maturing deciduous forest steep slope Bank=Shale Bedrock Indicate the dominant vegetation type(s) and record the dominant species
x Trees x Shrubs x Grasses Forbs Dominant species Sycamore and Red Oaks
INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width __51___m Fully open x Partly open Partly shaded Fully shaded Sampling reach area _510_m2
Estimated stream depth __135___m Proportion of Reach Represented by Stream High water mark __-___m Morphology Types Surface velocity (at thalweg) __20___msec x Riffle_40_ x Run_50_ x Pool 10 Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___0____ m2 Density of LWD ___-____ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation ___50____ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present Brown Algae
WATER QUALITY Temperature __17___degC Water Odors x NormalNone Sewage Specific Conductance ___-___ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ___-_____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used ____NA_______ Clear x Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other____None____ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 15 Detritus sticks wood coarse plant materials
0 Boulder gt256 mm (10rsquorsquo) 15
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 62 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 8 Sand 006-2 mm (gritty) 0 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Unnamed Tributary STATION HLR-4 STREAM CLASS LAT N 39 13035 (upstream) LONG W 078 05129 (upstream) LAT N 39 13000 (downstream) LONG W 078 05086 (downstream) FORM COMPILED BY Marie Dahl RIVER BASIN Potomac River INVESTIGATORS M Dahl HKepple SRobertson AFisher
DATE 31 May 2008 TIME 5 PM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality 2008
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) YES x NO
rain (steady rain) Air Temperature __22___degC showers (intermittent) Comments _______________________
_0__ cloud cover _0__ ______________________________
x clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area __-___ km2 mi2 Montane Significant springs andor seeps x Valley Marsh or swamp Montane amp Valley Mixture of origins Other ___________________
COMMENTS Snowden Bridge development (upstream) and sewer line
i
j
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence x FieldPasture Industrial Obvious sources Agricultural Other ____________ Construction upstream and adjacent x Residential Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left Forested with rocky banks field across silt fence and 2m tree strip Right Forested with some rocky banks and forest through the highway Indicate the dominant vegetation type(s) and record the dominant species
x Trees x Shrubs x Grasses Forbs Dominant species White Ash and Hackberry
INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width ___38__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area __380__m2
Estimated stream depth __12__cm Proportion of Reach Represented by Stream High water mark __2___m Morphology Types Surface velocity (at thalweg) __46___msec x Riffle__25___ x Run__70___ x Pool__5___ Channelized Yes x No Dam Present x Yes No
LARGE WOODY DEBRIS
LWD _______ m2 Density of LWD _______ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation ___NA____ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present___Algae___
WATER QUALITY Temperature __16___degC Water Odors x NormalNone Sewage Specific Conductance __-____ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ___-_____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used ____NA_____ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells x Other___Shale_______ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 0 Detritus sticks wood coarse plant materials
10 Boulder gt256 mm (10rsquorsquo) 5
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 45 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 30 Sand 006-2 mm (gritty) 0 Marl grey shell
fragments 0
Silt 0004-006 mm 20 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Lick Run STATION HLR-5 STREAM CLASS LAT N 39 127517 (upstream) LONG W 078 05685(upstream) LAT N 39 12784 (downstream) LONG W 078 05631 (downstream) FORM COMPILED BY B Millholland RIVER BASIN Potomac River INVESTIGATORS B Millholland JTovar SRobertson MDahl WBousquet JPangle
DATE 29 May 2008 TIME 1040 AM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project
k
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature __15___degC showers (intermittent) Comments _______________________
_5_ X cloud cover _0__ ______________________________
x clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane Significant springs andor seeps x Valley Marsh or swamp Montane amp Valley Mixture of origins Other ___________________
COMMENTS Lick Run appears to be intermittent and may be dry in Fall 2008
l
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence x FieldPasture Industrial Obvious sources Agricultural Other ____________ Adjacent logging stream bank scour Residential Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left 85 young deciduous forest 15 logged Bank=Soil vegetation and cobbles Right Young deciduous forest on 30 slope Bank= Soil vegetation and cobble Indicate the dominant vegetation type(s) and record the dominant species
x Trees Shrubs Grasses Forbs Dominant species Mixed Deciduous INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width __11__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area 110 m2
Estimated stream depth _009__m Proportion of Reach Represented by Stream High water mark __96___m Morphology Types Surface velocity (at thalweg) __01___msec x Riffle__30___ x Run__5___ x Pool__65___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___0____ m2 Density of LWD ___-____ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __70___ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present__________________________________________________________
WATER QUALITY Temperature __14___degC Water Odors x NormalNone Sewage Specific Conductance __-__ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ___-___ Water Surface Oils Slick Sheen Globs Flecks pH __-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used _____NA______ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other_____None_____
SEDIMENT AND SUBSTRATE
Odors Deposits Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other________________ x Other Slightly Salty Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 0 Detritus sticks wood coarse plant materials
5 Boulder gt256 mm (10rsquorsquo) 0
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 75 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 23 Sand 006-2 mm (gritty) 2 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION McCann Farm STATION HLR-6 STREAM CLASS LAT N 39 13076 (upstream) LONG W 078 06539 (upstream) LAT N 39 13063 (downstream) LONG W 078 06473 (downstream) FORM COMPILED BY MDahl RIVER BASIN Potomac River INVESTIGATORS MDahl JPangle SRobertson W Bousqet BMillholland JTovar H Kepple
DATE 30 May 2008 TIME 1050 AM (start)
PROJECT NAME
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) YES x NO
rain (steady rain) Air Temperature __18___degC showers (intermittent) Comments _______________________
_85_x cloud cover _10__ ______________________________
clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x (Significant springs andor seeps) x ( Valley) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS Multi-habitat sampling with D-frame nets and seine
m
n
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial x No evidence Some evidence x FieldPasture Industrial Obvious sources x Agricultural Other ____________ Minor stream bank scour Residential Local Watershed Erosion x None Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left 35 bank grasses scattered trees and some bushes Right High bank with grasses and scattered trees Indicate the dominant vegetation type(s) and record the dominant species
x Trees Shrubs Grasses Forbs Dominant species Black Walnut and Festuca elatior
INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width __2___m Fully open Partly open x Partly shaded Fully shaded Sampling reach area __200_m2
Estimated stream depth __03_m Proportion of Reach Represented by Stream High water mark __1___m Morphology Types Surface velocity (at thalweg) __166_msec x Riffle__0___ x Run__55___ x Pool__45___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___2____ m2 Density of LWD ___-____ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __20_____ x Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present ____Glyceria (grass)sp__________
WATER QUALITY Temperature __16___degC Water Odors x NormalNone Sewage Specific Conductance ___-___ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ____-____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used ______NA___ Clear x Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other__NONE__ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 0 Detritus sticks wood coarse plant materials
5 Boulder gt256 mm (10rsquorsquo) 0
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 27 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 38 Sand 006-2 mm (gritty) 5 Marl grey shell
fragments 0 (lt1)
Silt 0004-006 mm 25 Clay lt 0004 mm (slick) 5 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Appendix B
WATER QUALITY ANALYSIS THROUGH THE VIRGINIA
STREAM CONDITION INDEX (VSCI)
The Virginia Stream Condition Index (VSCI) relies on eight metrics to categorize stream health According to Burton and Gerritsen (2003 p 1-1) this index ldquohellip can be used as a primary indicator of ecosystem health and can identify impairment with respect to the reference (or natural) conditionrdquo These metrics are numeric measures of a biological communityrsquos species composition diversity ecological niches or tolerance to pollution
Table 13 on the next page describes each VSCI metric lists its formula and provides the best standard value (BSV) for that metric Based on a 100-point scale the BSV is the score that reflects optimum conditions for aquatic life ie the best water quality value possible (DEQ 2006b pp 8 33) The table also explains whether the score increases or decreases with environmental stress
As Table 13 indicates each of the eight VSCI metrics is calculated individually Each formula provides a unitless score based on a 100-point scale with 0 as the worst possible score and 100 as the best Any metric that provides a result above 100 is changed to 100 in order for the index to work properly After the eight metrics are calculated they are summed and averaged to provide a final VSCI score on a 100-point scale
Based on statistical analysis of Virginiarsquos streams and their BMI communities the DEQ (2006b p 8) currently uses the following tiers to interpret VSCI scores
VSCI Scores Aquatic Life Use
(ALI) Tiers
lt 42 severely stressed
42-55 moderately stressed
55-63 unknownfair (gray zone)
63-73 good
gt73 excellent
Section 2 of this report presents the results of calculations for the six BMI sampling sites the research team studied in the Hiatt-Lick Run subwatershed in 2008
o
p
1 Total taxa the number of distinct taxa in the sample measures the overall diversity of the macroinvertebrate assemblage The best standard value (BSV) is 189 Score = 100 x (X189) Total taxa decreases with stress 2 EPT taxa the sum of the distinct taxa in the orders Ephemeroptera (mayflies) Plecoptera (stoneflies) and Trichoptera (caddisflies) These orders are known to be sensitive to pollution The best standard value (BSV) is 117 Score = 100 x (X117) EPT taxa decreases with stress 3 Percent Ephemeroptera the percent abundance of individuals in the sample that belong to the order Ephemeroptera (mayflies) The best standard value (BSV) is 477 Score = 100 x (X477) Percent Ephemeroptera decreases with stress 4 Percent Plecoptera + Trichoptera less Hydropsychidae the percent abundance of individuals in the sample that belong to the orders Plecoptera (stoneflies) and Trichoptera (caddisflies) excluding the pollution-tolerant caddisfly family Hydropsychidae The best standard value (BSV) is 562 Score = 100 x (X562) Percent Plecoptera + Trichoptera less Hydropsychidae decreases with stress 5 Percent Scrapers the percent abundance of individuals in the sample whose primary functional mechanism for feeding is grazing on the substrate or periphyton (ie attached algae and associated material) The best standard value (BSV) is 467 Score = 100 x (X467) Percent scrapers decreases with stress 6 Percent Chironomidae the percent abundance of individuals in the sample that are Chironomidae (non-biting midge) larvae and pupae The best standard value (BSV) is 16 Score = 100 x [(100-X)(100-16)] Percent Chironomidae increases with stress 7 Percent Top Two Dominant the percent abundance of the two most frequent taxa present in the sample The best standard value (BSV) is 328 Score = 100 x [(100-X)(100-328)] The Percent Top 2 Dominant increases with stress 8 Hilsenhoff Biotic Index (HBI) an abundance-weighted average pollution tolerance of all the individuals within a sample keyed to the family level Tolerance ratings range from 1 (highly intolerant) to 10 (highly tolerant) The best standard value (BSV) is 29 Score = 100 x [(10-X)(10-29)] The Hilsenhoff Biotic Index (HBI) increases with stress ______________________________________________________________________________ Table 13 Description of Benthic Macroinvertebrate VSCI Metrics Includes responses (increases decreases) of each VSCI metric to degraded water quality conditions Adapted from Burton and Gerritsen (2003 pp 3-15 3-16 6-2) and DEQ (2008)
q
Appendix C
LABORATORY BENCH SHEETS
Shenandoah Universityrsquos Environmental Studies Department developed the Benthic Macroinvertebrate Laboratory Bench Sheet and VSCI Worksheet to record BMI specimen identifications and their VSCI classifications for each sample
This form is based in part on bench sheets developed by the EPA and Virginia DEQ for recording specimen identifications Additional columns on Shenandoah Universityrsquos forms for relative numbers and VSCI classifications make the calculation and verification of VSCI scores easier
Note the column for taxonomistrsquos initials Specimens were identified by students in Shenandoah Universityrsquos research group and then verified or where necessary changed by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality This external review helps assure the validity of the study Originals of these bench sheets with handwritten taxonomistsrsquo initials will be kept on file at Shenandoah Universityrsquos Environmental Studies Department for five years after the completion of this study
r
s
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-1 STATION NAME Mouth LAT N 39deg 12749rsquo (upstream) LONG W 078deg 04209rsquo (upstream) LAT N 39deg 12729rsquo (downstream) LONG W 078deg 04148rsquo (downstream) FORM COMPILED BY Jeremy Tovar W S Bousquet RIVER BASIN Potomac River INVESTIGATORS Marie Dahl Brandon Millholand J W Pangle Sean Robertson Jeremy Tovar Woodward Bousquet Ben Bousquet
Collection date 21 May 2008 Sheet completion date 12 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Hirudinea 3 33 JT BVW PR 8 SP Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Coenagrionidae 2 22 BVW PR 9 CR Ephemeroptera (mayflies) Caenidae 1 11 JT BVW CG 6 SP Ephemerllidae 4 44 JT BVW CG 2 CR Heptageniidae 5 55 JT BVW SC 3 CG Isonychiidae 1 11 BVW CF 3 CG Plecoptera (stoneflies) Perlidae 14 154 JT BVW PR 2 CR Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Corydalidae 1 11 JT BVW PR 5 CR Coleoptera (beetles) Elmidae 24 264 I JT BVW SC 4 CG Dytiscidae 4 44 JT BVW PR 6 GN Psephenidae 3 33 JT BVW SC 4 CG Diptera (true flies) Tipulidae 3 33 JT BVW SH 4 BU Chironomidae 26 286 JT BVW CG 6 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
t
Other Total Individuals 91 1001 Total Taxa 13
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
u
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-1 STATION NAME Mouth LAT N 39deg 12749rsquo (upstream) LONG W 078deg 04209rsquo (upstream) LAT N 39deg 12729rsquo (downstream) LONG W 078deg 04148rsquo (downstream) FORM COMPILED BY Jeremy Tovar W S Bousquet RIVER BASIN Potomac River INVESTIGATORS Brandon Millholand JW Pangle Jeremy Tovar Heather Kepple
Collection date 10 October 2008 Sheet completion date 12 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Oligochaeta 3 35 JT BVW CG 8 BU Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Coenagrionidae 2 23 JT BVW PR 9 CR Ephemeroptera (mayflies) Heptageniidae 12 140 JT BVW SC 3 CG Isonychiidae 2 23 BVW CF 3 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Hydropsychidae 16 186 JT BVW CF 6 CG Philopotamidae 31 360 JT BVW CF 2 CG Hemiptera (true bugs) (Veliidae) (5) - JT BVW ( ) Megaloptera (dobsonflies etc) Corydalidae 10 116 JT BVW PR 5 CR Coleoptera (beetles) Dytiscidae 1 12 JT BVW PR 6 GN Psephenidae 7 81 JT BVW SC 4 CG Diptera (true flies) Tipulidae 2 23 BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
v
Other Total Individuals 86
(91) 999
Total Taxa 10 (11)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009 Veliidae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
w
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-2 STATION NAME Package Plant LAT N 39deg 12958rsquo (upstream) LONG W 078deg 04900 (upstream) LAT N 39deg 12958rsquo (downstream) LONG W 078deg 04822 (downstream) FORM COMPILED BY JW Pangle W Bousquet RIVER BASIN Potomac River INVESTIGATORS JW Pangle S Robertson J Tovar M Dahl B Millholland W Bousquet
Collection date 29 May 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Plecoptera (stoneflies) Perlidae 11 136 I JP BVW PR 1 CR Trichoptera (caddisflies) Hydropsychidae 10 123 I JP BVW CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 14 173 I JP BVW SC 4 CG Diptera (true flies) Chironomidae 32 395 I JP BVW CG 6 BU Simuliidae 14 173 I JP BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
x
Other Total Individuals 81 1000 Total Taxa 5
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-2 STATION NAME Package Plant LAT N 39deg 12958rsquo (upstream) LONG W 078deg 04900 (upstream) LAT N 39deg 12958rsquo (downstream) LONG W 078deg 04822 (downstream) FORM COMPILED BY JW Pangle W Bousquet RIVER BASIN Potomac River INVESTIGATORS JW Pangle Brandon Millholland Jeremy Tovar Heather Kepple
Collection date 10 October 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Heptageniidae 40 392 I WBJP SC 3 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Hydropsychidae 7 69 I JP WB BVW CF 6 CG Philopotamidae 28 275 I JP WB BVW CF 2 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Corydalidae 6 59 I JP WB BVW PR 5 CR Coleoptera (beetles) Psephenidae 7 69 I JP WB BVW SC 4 CG Elmidae 8 78 I JP WB BVW SC 4 CG Diptera (true flies) Tipulidae 6 59 I JP WB BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
y
z
Other Total Individuals 102 1001 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-3 STATION NAME Rt 664 Bridge LAT N 39deg 12934rsquo (upstream) LONG W 078deg 05019rsquo (upstream) LAT N 39deg 12951rsquo (downstream) LONG W 078deg 04958rsquo (downstream) FORM COMPILED BY S Robertson W Bousquet RIVER BASIN Potomac River INVESTIGATORS SRobertson BMillholland WBousquet MDahl JPangle
Collection date 28 May 2008 Sheet completion date 11 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Leptophlebiidae 1 15 I SGR BVW CG 2 CR Baetidae 1 15 I BVW CG 5 CG Plecoptera (stoneflies) Perlidae 3 46 I SGR BVW PR 1 CR Nemouridae 1 15 I BVW SH 3 CR Trichoptera (caddisflies) Hydropsychidae 5 77 I SGR BVW CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 27 415 I SGR BVW SC 4 CG Psephenidae 2 31 A SGR BVW SC 4 CG Diptera (true flies) Simuliidae 5 77 I SGR BVW CF 6 CG Chironomidae 20 308 I SGR BVW CG 6 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
aa
bb
Other Total Individuals 65 999 Total Taxa 9
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-3 STATION NAME Rt 664 Bridge LAT N 39deg 12934rsquo (upstream) LONG W 078deg 05019rsquo (upstream) LAT N 39deg 12951rsquo (downstream) LONG W 078deg 04958rsquo (downstream) FORM COMPILED BY S Robertson RIVER BASIN Potomac River INVESTIGATORS S Robertson M Dahl A Fisher H Kepple
Collection date 10 Oct 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Heptageniidae 2 20 I SGR SC 3 CG Isonychiidae 2 20 I BVW CF 2 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Philopotamidae 36 367 I SGR CF 2 CG Hydropsychidae 47 480 I SGR CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 2 20 A SGR SC 4 CG Psephenidae 8 82 A SGR SC 4 CG Diptera (true flies) Tabanidae 1 10 I SGR PR 7 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
cc
dd
Other Total Individuals 98 999 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-4 STATION NAME Unnamed Tributary LAT (upstream) N 39deg 13035rsquo LONG (upstream) W 078deg 05129rsquo LAT (downstream) N 39deg 13000rsquo LONG (downstream) W 078deg 5086rsquo FORM COMPILED BY A Fisher M Dahl rev W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson A Fisher H Kepple
Collection date 31 May 2008 Sheet completion date rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Flatworms (Turbellaria) Worms (Oligochaeta) Naididae 1 14 - BVW CG 8 BU Leeches (Hirudinea) Snails (Gastropoda) Clams (Bivalvia) Isopods (Isopoda) Asellidae 3 42 A AF BVW CG 8 SP Scuds (Amphipoda) Crayfish Shrimp (Decapoda) Water Mites (Hydracarina) Dragonflies (Odonata Anisoptera) Damselflies (Odonata Zygoptera) Mayflies (Ephemeroptera) Stoneflies (Plecoptera) Perlidae 2 28 I AF BVW PR 1 CR Caddisflies (Trichoptera) True Bugs (Hemiptera) Corixidae 1 14 I AF BVW MP 9 DV Dobsonflies (Megaloptera) Beetles (Coleoptera) Dytiscidae 1 14 I AF BVW PR 6 GN True Flies (Diptera) Chironomidae 61 847 I AF BVW CG 6 BU Simuliidae 3 42 I AF BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
ee
ff
Other Total Individuals 72 1001 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-4 STATION NAME Unnamed Tributary LAT (upstream) N 39deg 13035rsquo LONG (upstream) W 078deg 05129rsquo LAT (downstream) N 39deg 13000rsquo LONG (downstream) W 078deg 5086rsquo FORM COMPILED BY Andrew G M Fisher W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson A Fisher H Kepple
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Flatworms (Turbellaria) Worms (Oligochaeta) Lumbriculidae 2 32 A AF BVW CG 8 BU Leeches (Hirudinea) Snails (Gastropoda) Lymnaeidae 1 16 A BVW CG 9 GN Clams (Bivalvia) Isopods (Isopoda) Scuds (Amphipoda) Crayfish Shrimp (Decapoda) Cambaridae 1 16 A AF BVW GN 5 GN Water Mites (Hydracarina) Dragonflies (Odonata Anisoptera) Damselflies (Odonata Zygoptera) Calopterygidae 4 63 I BVW PR 7 CL Mayflies (Ephemeroptera) Tricorythidae 1 16 I BVW CG 5 SP Stoneflies (Plecoptera) Caddisflies (Trichoptera) Phryganeidae 4 63 I AF BVW SH 4 CR Hydropsychidae 6 95 I AF BVW CF 6 CG I True Bugs (Hemiptera) Corixidae 8 127 I AF BVW MP 9 DV (Veliidae ) (7) - I AF BVW ( ) Dobsonflies (Megaloptera) Sialidae 3 48 I AF BVW PR 7 SP Corydalidae 2 32 I AF BVW PR 5 CR Beetles (Coleoptera) Haliplidae 1 16 A AF BVW MP 8 GN Dysticidae 7 111 I AF BVW PR 6 GN Hydrophilidae 1 16 A AF BVW PR 8 GN Elmidae 3 48 I BVW SC 4 CG True Flies (Diptera) Chironomidae 8 127 I AF BVW CG 6 BU Simuliidae 7 111 I AF BVW CF 6 CG Tipulidae 4 63 I AF BVW SH 4 GU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
gg
hh
Other Total Individuals 63
(70) 1000
Total Taxa 17 (18)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Veliidae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-5 STATION NAME Lick Run LAT N 39deg 12752rsquo (upstream) LONG W 078deg 05685rsquo (upstream) LAT N 39deg 12784rsquo (downstream) LONG W 078deg 05631rsquo (downstream) FORM COMPILED BY Brandon Millholland W Bousquet RIVER BASIN Potomac River INVESTIGATORS J Pangle S Robertson W Bousquet JTovar M Dahl
Collection date 29 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Gomphidae 1 15 I BVW PR 3 BU Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Leptophlebiidae 11 167 A LBM BVW CG 2 CR Plecoptera (stoneflies) Perlidae 10 152 A LBM BVW PR 1 CR Perlodiae 1 15 A LBM BVW PR 2 CR Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 5 76 A LBM BVW SC 4 CG Elmidae 2 30 I LBM BVW SC 4 CG Diptera (true flies) (Culicidae) (3) - P LMB BVW ( ) Chironomidae 23 348 I LBM BVW CG 6 BU Simuliidae 13 197 I LBM BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
ii
jj
Other Total Individuals 66
(69) 1000
Total Taxa 7 (8)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Culicidae (Diptera) pupae not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl J Pangle S Robertson W Bousquet B Millholland J Tovar H Kepple
Collection date 30 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 1 12 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Asellidae 114 965 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Plecoptera (stoneflies) Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 2 23 A MRD BVW SC 4 CG Diptera (true flies)
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
kk
ll
Other Total Individuals 117 1000 Total Taxa 3
Notes Subsample taken 29 October 2009 All taxonomic determinations by MRD were confirmed by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson K McCann A Fisher H Kepple A Barrett
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 2 17 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Sphaeriidae 2 17 I MRD BVW CF 8 BU Isopoda (isopods) Asellidae 99 853 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Calopterygidae 2 17 A MRD BVW PR 7 CL Ephemeroptera (mayflies) Leptophlebiidae 2 17 I MRD BVW CG 2 CR Heptageniidae 4 34 A MRD BVW SC 3 CG Plecoptera (stoneflies) Perlidae 1 09 A MRD BVW PR 1 CR Trichoptera (caddisflies) Hemiptera (true bugs) (Gerridae) (1) - A MRD BVW ( ) Corixidae 1 09 A MRD BVW PR 5 CR Megaloptera (dobsonflies etc) Coleoptera (beetles) Dryopidae 1 09 A MRD BVW SC 5 CG Diptera (true flies) Tipulidae 2 17 I BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
mm
nn
Other Total Individuals 116
(117) 999
Total Taxa 10 (11)
Notes Subsample taken 6 November 2008 Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009 Gerridae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
- Disclaimer ii
- Title Page iii
- Acknowledgements v
- Table of Contents vii
- SECTION 2 RESULTS DATA TABLES 7
- OVERVIEW AND FINDINGS
- Introduction
- RESULTS DATA TABLES
- REFERENCES
- FIELD DATA SHEETS
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- SubstrateType
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- WATER QUALITY ANALYSIS
- THROUGH THE VIRGINIA
- STREAM CONDITION INDEX (VSCI)
- LABORATORY BENCH SHEETS
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
vi
TABLE OF CONTENTS page
Disclaimer ii Title Page iii Acknowledgements v Table of Contents vii SECTION 1 OVERVIEW AND FINDINGS 1 Introduction 1 Methods and Analysis 3 Findings 3 Future Research 5 SECTION 2 RESULTS DATA TABLES 7 Site HLR-1 Mouth 7 Site HLR-2 Package Plant 8 Site HLR-3 Route 664 Bridge 9 Site HLR-4 Unnamed Tributary 10 Site HLR-5 Lick Run 11 Site HLR-6 McCann Farm 12 REFERENCES 13 APPENDICES 15 Appendix A Field Data Sheets Appendix B Water Quality Analysis through the Virginia Stream Condition Index (VSCI) Appendix C Laboratory Bench Sheets Note Photographs of the six sampling sites appear on a CD provided separately to the grant administrator
vii
viii
Section 1
OVERVIEW AND FINDINGS
Introduction
This report presents baseline water quality data for Hiatt Run and Lick Run -- termed the Hiatt-Lick Run subwatershed in this study Located in Frederick County Virginia Hiatt and Lick Runs are tributaries of Opequon Creek The Opequon in turn empties into the Potomac River and then the Chesapeake Bay The investigation was conducted by a Shenandoah University research team in 2008 Researchers used benthic macroinvertebrates (BMIs) as biological indicators of water quality This report presents an overview of the study followed by summary tables and a list of references The Appendix contains data sheets and a description of the data analysis These data will form part of the baseline water quality assessment that is being undertaken through the Opequon Creek Targeted Watershed Grant The grant administered by Jim Lawrence reflects a broad-based partnership that includes Virginia Tech Shenandoah University the Frederick-Winchester Service Authority The Opequon Watershed (TOW) citizens organization as well as federal state and local governments community groups and business interests Grant partners will use best management practices (BMPs) to reduce nutrient runoff and address other pollution problems in the Opequon Creek watershed Since 1996 Opequon Creek has appeared on the impaired waters list prepared by the Virginia Department of Environmental Quality (DEQ) The Hiatt-Lick Run subwatershed was selected for this study because it represents changes that are typical of the Opequon Creek watershed These changes include new commercial and residential developments as well as the implementation of water quality improvement practices such as tree planting in riparian zones fences to prevent livestock from entering streams and replacing outdated sewage treatment facilities such as a package plant on lower Hiatt Run
Seven students from Shenandoah Universityrsquos Environmental Studies Department conducted this study from May through December 2008 under the direction of Woodward Bousquet Professor of Environmental Studies and Biology The research team followed procedures described in the Rapid Bioassessment Protocols (RBPs) of the US Environmental Protection Agency (Barbour et al 1999) and the Virginia Stream Condition Index (VSCI) The VSCI provides an evaluation of water quality based on a streamrsquos benthic macroinvertebrate community (Burton and Gerritsen 2003 DEQ 2006b and 2008)
In consultation with Billy van Wart of the DEQ and grant administrator Jim Lawrence the Shenandoah University researchers selected six representative sampling sites in the Hiatt-Lick Run subwatershed Each site was given a name and an HLR (for Hiatt-Lick Run) code Figure 1 maps their positions while their characteristics and coordinates appear in the field data sheets (see Appendix A) These sites were established according to EPA and DEQ guidelines that generally call for a 100-meter-long stream reach with at least three riffle zones a width sufficient to accommodate a 1-meter seine and a substrate composed of least 35 cobbles
Specific locations of the sampling sites on Hiatt and Lick Runs were selected to reflect different watershed positions and changing land uses See Figure 1 Further information about each sampling site appears in the field data sheets (Appendix A)
1
Figure 1 Sampling Sites in the Hiatt-Lick Run Subwatershed of Opequon Creek Frederick County Virginia Scale 1 centimeter = 500 meters
HLR-1 (Mouth) measures water quality at the lowest point of the subwatershed as Hiatt Run flows into Opequon Creek Forests cover the immediate riparian zones on both sides of Hiatt Run This site will be used in future years by the DEQ for BMI monitoring HLR-2 (Package Plant) is situated on Hiatt Run just below the outflow pipe of an outdated package plant that formerly treated sewage from the Jordan Springs area During the study period the package plant was taken off-line and a pumping station was constructed The Frederick-Winchester Service Authority now treats this sewage at its Opequon Water Reclamation Facility HLR-3 (Route 664 Bridge) was chosen because it is located upstream from the package plant but downstream of the confluence of Hiatt Run and Lick Run HLR-4 (Unnamed Tributary) is located downstream of the Snowden Bridge residential development which has been under construction The area immediately upstream of this sampling site was being graded by heavy equipment during the May 2008 sampling time HLR-5 (Lick Run) the researchers learned dries up in late summer It had a modest but constant flow during when the May 2008 sample was taken HLR-6 (McCann Farm) represents the headwater areas of the Hiatt-Lick Run subwatershed of Opequon Creek The McCanns who graze cattle in their pastures that surround Hiatt Run here have been working with the Potomac Conservancy to implement Best Management Practices
2
(BMPs) to improve water quality These practices include fencing cattle out of the stream and spring runs and planting more trees in the riparian zone
Methods and Analysis
The research team established a sampling plan and then sampled according to methods the DEQ developed for the VSCI Using a 1 meter x 1 meter seine samples from 2m2 of substrate in riffle zones were to be collected twice at each site once in May 2008 and once in October 2008 At five of the six sites stream characteristics permitted the standard single-habitat sampling procedure At the McCann Farm (Site HLR-6) researchers needed to use multi-habitat sampling See Barbour et al (1999 pp 7-3 ff) for further descriptions of these two methods
All six sites were sampled in May 2008 Low-water conditions in October 2008 prevented the Shenandoah University group from collecting BMIs at Lick Run (HLR-5) that month but the other five sites were sampled successfully As a result the research team obtained eleven samples for analysis in this study Samples were preserved in ethanol for subsequent identification
DEQ procedures require subsampling when the number of BMIs in a given sample exceeds 100 All eleven samples in this study met that criterion Consequently researchers used a Caton tray to produce random subsamples of 90 to 110 specimens from each sample During the analysis that followed some specimens in the subsamples were rejected either because they turned out to be terrestrial species or because they were in the wrong life stage (eg pupae) In most cases the researchers identified specimens to the family level Primary taxonomic references were A Guide to Common Freshwater Invertebrates of North America (Voshell 2002) Fresh-Water Invertebrates of the United States (Pennak 1953) and An Introduction to the Aquatic Insects of North America (Merritt and Cummins 1996) DEQ Regional Aquatic Biologist Billy van Wart reviewed verified and where necessary corrected the identifications
The investigators then used the Virginia Stream Condition Index (VSCI) to produce water quality scores based on the BMIs collected A VSCI score is based on eight different measurement formulas called metrics Each metric is a numeric measure of a biological communityrsquos species composition diversity ecological niches or tolerance to pollution When combined into a single multimetric VSCI score the response of the benthic community to water conditions can be evaluated For further information and worksheets see Appendices B and C respectively
Findings
Table 1 presents the VSCI scores for the eleven samples collected in the Hiatt-Lick Run subwatershed in 2008 Since a sample year consists of two samples the mean scores for each site for the entire year are also included As the table shows
bull None of the 11 samples was rated excellent bull Only 1 of the 11 samples was rated as good bull Only 2 of the 11 samples were rated fair (ie in the ldquogrey zonerdquo between impaired and
unimpaired) bull The remaining 8 samples were in either moderately stressed or severely stressed
condition
3
Site Name and Code Sampling
Date of
TaxaVCSI Score
Water Quality Rating
Mouth (HLR-1) 21 May 2008 13 571 Fair (Gray Zone)
Mouth (HLR-1) 10 Oct 2008 10 608 Fair (Gray Zone)
site average for 2008 590 Fair (Gray Zone)
Package Plant (HLR-2) 29 May 2008 5 370 Severely Stressed
Package Plant (HLR-2) 10 Oct 2008 7 674 Good
site average for 2008 522 Moderately Stressed
Route 664 Bridge (HLR-3) 28 May 2008 9 485 Moderately Stressed
Route 664 Bridge (HLR-3) 10 Oct 2008 7 469 Moderately Stressed
site average for 2008 477 Moderately Stressed
Unnamed Tributary (HLR-4) 31 May 2008 7 173 Severely Stressed
Unnamed Tributary (HLR-4) 10 Oct 2008 17 476 Moderately Stressed
site average for 2008 325 Severely Stressed
Upper Lick Run (HLR-5) 29 May 2008 7 455 Moderately Stressed
site average for 2008 455 Moderately Stressed
McCann Farm (HLR-6) 30 May 2008 3 190 Severely Stressed
McCann Farm (HLR-6) 10 Oct 2008 10 316 Severely Stressed
site average for 2008 253 Severely Stressed
Summary 6 sites 11 samples
36 BMI taxa
VSCI = Virginia Stream Condition Index see Burton and Gerritsen (2003) and Virginia Department of Environmental Quality (2006b and 2008) Table 1 Summary Table of Virginia Stream Condition Index (VSCI) Scores and Water Quality Ratings for Sites in the Hiatt-Lick Run Subwatershed of Opequon Creek Frederick County Virginia 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
4
Improvements in water quality ratings at three sites between May and October suggest that BMPs and other changes may already be having some positive effects on water quality At the Package Plant (Site HLR-2) the VSCI score rose dramatically from 370 (severely stressed) to 674 (good) The small-scale sewage treatment plant as noted above was taken off-line during this period Similarly water quality at the Unnamed Tributary (HLR-4) increased from 173 (severely stressed) to 476 (moderately stressed) Adjacent construction was completed over the summer and grass was planted on the property Management practices on the McCann Farm (HLR-6) may be responsible for improving conditions for aquatic life in Hiatt Runrsquos headwaters Even though the water quality was still rated as severely stressed in October the VSCI score rose from 190 to 316 at this sampling site Further studies are needed to determine whether the changes noted in 2008 persist into the future In 2003 the DEQ began a Total Maximum Daily Load (TMDL) process for Opequon Creek as required by the Water Quality Act of 1987 The TMDL approach establishes a ldquopollution budgetrdquo for each impaired stream for each type of impairment and for each responsible pollutant In conjunction with the DEQ and the Virginia Department of Conservation and Recreation (DCR) Virginia Polytechnic Institutersquos Department of Biosystems Engineering developed a TMDL implementation plan (Opequon Creek IP Steering Committee et al 2006) Monitoring the BMI communities of Opequon Creek and its tributaries is essential to evaluating the progress of the proposed water quality improvement projects
Future Research The relatively small Hiatt-Lick Run subwatershed provides several case studies that can help assess the effectiveness of various water quality management practices not only in Opequon Creek but also in other watersheds in the Chesapeake Bay region and beyond In supporting that goal this study makes two principal contributions 1 It provides baseline data to measure the improvement or degradation of the Hiatt-Lick Run section of the Opequon Creek watershed and 2 It establishes a monitoring framework for the long-term biological monitoring of water quality and the evaluation of best management practices (BMPs) This framework consists of
bull Sampling Sites ndash six stream reaches in the Hiatt-Lick Run subwatershed selected as sampling sites according to EPA and DEQ specifications
bull Data Collection Protocols ndash based on the EPArsquos Rapid Bioassessment Protocols (RBPs)
(Barbour et al 1999) and the DEQrsquos Virginia Stream Index (VSCI) (Burton and Gerritsen 2003 DEQ 2006b and 2008) and
bull Analytical Procedures ndash contained in the Virginia Stream Condition Index (VSCI see
Burton and Gerritsen 2003 DEQ 2006b pp 8 and 33 and DEQ 2008 pp 29-34)
5
6
Section 2
RESULTS DATA TABLES
Site HLR-1 Mouth
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 13 189 6872 EPT taxa 5 117 4273 Ephemeroptera 121 477 2544 Plec +Tric less Hydropsych 154 562 2745 Scrapers 352 467 7536 Chironomidae 286 16 7267 Top 2 Dominant 550 328 6708 Hilsenhoff Biotic Index (HBI) 45 29 775VSCI Score 571
According to the DEQrsquos water quality standards aquatic life at this site is fair (gray zone) See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 2 VSCI Scores for Mouth (HLR-1) Site in Hiatt-Lick Run Sampled on 21 May 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 10 189 5292 EPT taxa 4 117 3423 Ephemeroptera 163 477 3424 Plec +Tric less Hydropsych 360 562 6415 Scrapers 221 467 4736 Chironomidae 00 16 10007 Top 2 Dominant 546 328 6768 Hilsenhoff Biotic Index (HBI) 39 29 859VSCI Score 608
According to the DEQrsquos water quality standards aquatic life at this site is fair (gray zone) See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 3 VSCI Scores for Mouth (HLR-1) Site in Hiatt-Lick Run Sampled 10 October 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
7
Site HLR-2 Package Plant
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 5 189 2642 EPT taxa 2 117 1713 Ephemeroptera 00 477 004 Plec +Tric less Hydropsych 110 562 1965 Scrapers 173 467 3706 Chironomidae 395 16 6157 Top 2 Dominant 568 328 6438 Hilsenhoff Biotic Index (HBI) 50 29 704VSCI Score 370
According to the DEQrsquos water quality standards aquatic life at this site is severely stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 4 VSCI Scores for Package Plant (HLR-2) Site in Hiatt-Lick Run Sampled 29 May 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 7 189 3702 EPT taxa 34 117 2563 Ephemeroptera 392 477 8214 Plec +Tric less Hydropsych 275 562 4895 Scrapers 539 467 10006 Chironomidae 00 16 10007 Top 2 Dominant 667 328 4968 Hilsenhoff Biotic Index (HBI) 32 29 958VSCI Score 674
According to the DEQrsquos water quality standards aquatic life at this site is good See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 5 VSCI Scores for Package Plant (HLR-2) Site in Hiatt-Lick Run Sampled 10 October 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
8
Site HLR-3 Route 664 Bridge
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 9 189 4762 EPT taxa 5 117 4273 Ephemeroptera 30 477 634 Plec +Tric less Hydropsych 61 562 1095 Scrapers 446 467 9556 Chironomidae 308 16 7037 Top 2 Dominant 723 328 4128 Hilsenhoff Biotic Index (HBI) 48 29 732VSCI Score 485
According to the DEQrsquos water quality standards aquatic life at this site is moderately stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 6 VSCI Scores for Route 664 Bridge (HLR-3) Site in Hiatt-Lick Run Sampled 28 May 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 7 189 3702 EPT taxa 4 117 3423 Ephemeroptera 40 477 844 Plec +Tric less Hydropsych 367 562 6535 Scrapers 122 467 2616 Chironomidae 00 16 10007 Top 2 Dominant 847 328 2288 Hilsenhoff Biotic Index (HBI) 42 29 817VSCI Score 469
According to the DEQrsquos water quality standards aquatic life at this site is moderately stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 7 VSCI Scores for Route 664 Bridge (HLR-3) Site in Hiatt-Lick Run Sampled 10 October 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
9
Site HLR-4 Unnamed Tributary
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 7 189 3702 EPT taxa 1 117 853 Ephemeroptera 00 477 004 Plec +Tric less Hydropsych 28 562 495 Scrapers 00 467 006 Chironomidae 847 16 1557 Top 2 Dominant 889 328 1658 Hilsenhoff Biotic Index (HBI) 60 29 563VSCI Score 173
According to the DEQrsquos water quality standards aquatic life at this site is severely stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 8 VSCI Scores for Unnamed Tributary (HLR-4) Site in Hiatt-Lick Run Sampled 31 May 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 17 189 8992 EPT taxa 3 117 2563 Ephemeroptera 16 477 344 Plec +Tric less Hydropsych 63 562 1125 Scrapers 48 467 1036 Chironomidae 127 16 8877 Top 2 Dominant 254 328 10008 Hilsenhoff Biotic Index (HBI) 63 29 521VSCI Score 476
According to the DEQrsquos water quality standards aquatic life at this site is moderately stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 9 VSCI Scores for Unnamed Tributary (HLR-4) Site in Hiatt-Lick Run Sampled 10 October 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
10
Site HLR-5 Lick Run
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 7 189 3702 EPT taxa 3 117 2563 Ephemeroptera 167 477 3504 Plec +Tric less Hydropsych 167 562 2975 Scrapers 106 467 2276 Chironomidae 348 16 6637 Top 2 Dominant 545 328 6778 Hilsenhoff Biotic Index (HBI) 43 29 803VSCI Score 455
According to the DEQrsquos water quality standards aquatic life at this site is moderately stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 10 VSCI Scores for Lick Run (HLR-5) Site in Hiatt-Lick Run Sampled 29 May 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
Note
Water conditions at the Lick Run (HLR-5) site were too low in October 2008 to permit a sample to be taken
11
Site HLR-6 McCann Farm
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 3 189 1592 EPT taxa 0 117 003 Ephemeroptera 00 477 004 Plec +Tric less Hydropsych 00 562 005 Scrapers 23 467 496 Chironomidae 00 16 10007 Top 2 Dominant 988 328 188 Hilsenhoff Biotic Index (HBI) 79 29 296VSCI Score 190
According to the DEQrsquos water quality standards aquatic life at this site is severely stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 11 VSCI Scores for McCann Farm (HLR-6) Site in Hiatt-Lick Run Sampled 30 May 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 10 189 5292 EPT taxa 3 117 2563 Ephemeroptera 51 477 1074 Plec +Tric less Hydropsych 09 562 165 Scrapers 43 467 926 Chironomidae 00 16 10007 Top 2 Dominant 887 328 1688 Hilsenhoff Biotic Index (HBI) 75 29 352VSCI Score 315
According to the DEQrsquos water quality standards aquatic life at this site is severely stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 12 VSCI Scores for McCann Farm (HLR-6) Site in Hiatt-Lick Run Sampled 10 October 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
12
REFERENCES
Barbour Michael T Jeroen Gerritsen Blaine D Snyder and James B Stribling 1999 Rapid bioassessment protocols for use in streams and wadeable rivers periphyton benthic macroinvertebrates and fish Second edition EPA 841-B-99-002 Washington DC US Environmental Protection Agency Office of Water Available online at httpwwwepagovOWOWmonitoringbioasspshtml Burton June and Jeroen Gerritsen 2003 A stream condition index for Virginia non-coastal streams Prepared for the US EPA Office of Science and Technology US EPA Region 3 Environmental Services Division and Virginia Department of Environmental Quality Owings Mills Maryland Tetra Tech Inc Available online at httpwwwdeqvirginiagovwatermonitoringpdf vastrmconpdf Hessler Rachel A Andrea R Martin Heidi D Ruggeri William B Seymour Jr and Woodward S Bousquet 2006 Water quality assessment using benthic macroinvertebrates A study and compilation Winchester Virginia Environmental Studies Program Shenandoah University Occasional publication no 10 Igoe Robert 2006 DEQ identifies nine impaired streams in Clarke Frederick The Winchester Star 15 July B1 Jackson Charlie 2005 Survey targets Opequon cleanup The Winchester Star 19 August B1 Johnston Elizabeth A and Woodward S Bousquet 2003 What happens when it rains A study of water quality in Abrams Creek and Town Run Winchester and Frederick County Virginia Winchester Virginia Environmental Studies Program Shenandoah University Occasional publication no7 Merritt Richard W and Kenneth W Cummins 1996 An introduction to the aquatic insects of North America Dubuque Iowa KendallHunt Publishing Company Opequon Creek IP Steering Committee Virginia Tech Department of Biological Systems Engineering and Center for TMDL and Watershed Studies University of Virginia Institute for Environmental Negotiation and West Virginia University Division of Resource Management 2006 Opequon Creek watershed TMDL implementation plan Blacksburg Virginia Virginia Tech Department of Biological Systems Engineering Available online at httpwwwtmdlbsevteduoutreachC82 Pennak Robert W 1953 Fresh-water invertebrates of the United States New York New York The Ronald Press Company Rosenburg David M and Vincent H Resh 1993 Freshwater biomonitoring and benthic macroinvertebrates New York New York Chapman amp Hall Shenandoah University Public Relations Office 2002 Data collected by SU and its partners helps identify impaired water Winchester Virginia Available online at httpwwwsuedutemp_ newscfmurlnum=32 Thorp James H and Alan P Covich 1991 Ecology and classification of North American freshwater invertebrates San Diego California Academic Press Inc
13
US Environmental Protection Agency 1997 Volunteer stream monitoring a methods manual EPA 841-B-97-003 Washington DC US Environmental Protection Agency Office of Water Van Meter Val 2003 Opequon Creek pollution limits to be established The Winchester Star 4 April B1 Virginia Department of Environmental Quality 2006a Virginia 305(b)303(d) water quality integrated report to Congress and the EPA Administrator for the period January 1 2000 to December 31 2004 Richmond Virginia Virginia Department of Environmental Quality Available online at httpwwwdeqvirginiagovwqair2006html __________ 2006b Using probabilistic data to validate the non-coastal Virginia Stream Condition Index (draft) VDEQ technical bulletin WQA2006-001 Virginia Department of Environmental Quality Available online at httpwwwloudounwatershedorgstream_assessmentreference_documents VA_DEQ_SCI_Validationpdf __________ 2008 Biological monitoring program quality assurance project plan for wadeable streams and rivers Richmond Virginia Virginia Department of Environmental Quality Update available online at httpwwwdeqstatevausbiomonitoringdocumentsBioMonQAPP_13Aug2008pdf Virginia Tech Aquatic Entomology Laboratory 2005 Taxa dictionary [Excel file] Blacksburg Virginia Virginia Tech Department of Biological Systems Engineering 2003a Opequon watershed TMDLs for benthic impairments Abrams Creek and Lower Opequon Creek Frederick and Clarke Counties Virginia Prepared for the Virginia Department of Environmental Quality and Virginia Department of Conservation and Recreation Blacksburg Virginia Available online at httpwwwdeqstate vaustmdlapptmdlsshenrvrabropebcpdf Voshell J Reese Jr 2002 A guide to common freshwater invertebrates of North America Blacksburg Virginia The McDonald amp Woodward Publishing Company
14
APPENDICES
15
16
a
Appendix A
FIELD DATA SHEETS
This appendix presents a Site Characterization Field Data Sheet for each of the six sampling reaches established on Hiatt Run and Lick Run for this study They were filled out during the sampling runs made in May 2008
These forms were adapted by Shenandoah University from forms used by the EPA and Virginia DEQ for recording characteristics of sampling sites
b
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Mouth STATION _HLR-1_____ STREAM CLASS LAT N 39 12749 (upstream) LONG W 078 04209(upstream) LAT N 39 12729 (downstream) LONG W 078 04148 (downstream) FORM COMPILED BY Jeremy Tovar RIVER BASIN Potomac River INVESTIGATORS JPangle B Millholland MDahl SRobertson W Bousquet JTovar BBousquet
DATE May 21 2008_________ TIME_250 PM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature __24___degC showers (intermittent) Comments _______________________
___ cloud cover _0_ ______________________________
X clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x ( Significant springs andor seeps) x (Valley ) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS
c
d
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence x FieldPasture Industrial Obvious sources Agricultural Other ____________ Stream scour steep slope erosion x Residential Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left Field and young thin deciduous forest 100 Bank=Grass cobble and shrub Right 90 maturing deciduous forest 45 shale slope Bank=Bedrock soil and herbs Indicate the dominant vegetation type(s) and record the dominant species
x Trees Shrubs x (Grasses) Forbs Dominant species Oak species and mix deciduous
INSTREAM FEATURES
Measured reach length ___100__m Canopy Cover Estimated stream width _35__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area __350_m2
Estimated stream depth _008_m Proportion of Reach Represented by Stream High water mark _15_m Morphology Types Surface velocity (at thalweg) _05__msec x Riffle__75___ x Run__20___ x Pool__5___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___00__ m2 Density of LWD _-_ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __15_____ x Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present Algae
WATER QUALITY Temperature __19___degC Water Odors x NormalNone Sewage Specific Conductance - Petroleum Chemical Fishy Other___________________ Dissolved Oxygen - Water Surface Oils Slick Sheen Globs Flecks pH __-_ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used _____NA________ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic x None Relict shells Other______None_____ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 8 Detritus sticks wood coarse plant materials
0 Boulder gt256 mm (10rsquorsquo) 0
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 51 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 40 Sand 006-2 mm (gritty) 1 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University
SITE CHARACTERIZATION FIELD DATA SHEET FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Package Plant STATION HLR-2 STREAM CLASS LAT N 39 129580 (upstream) LONG W 078 04900 (upstream) LAT N 39 129581 (downstream) LONG W 078 04822 (downstream) FORM COMPILED BY JW Pangle RIVER BASIN Potomac River INVESTIGATORS JPangle JTovar MDahl B Millholland SRobertson W Bousquet
DATE__19 May 2008_ TIME__1240__ PM
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature _____degC showers (intermittent) Comments __Sunny Day_____________
_5__ cloud cover _0__ ______________________________
x clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x (Significant springs andor seeps) x (Valley) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS
e
f
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence FieldPasture Industrial Obvious sources Agricultural Other ____________ __Upstream Construction and Stream bank Scour Residential __________________________________ Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left Deciduous forest with thick herb and shrub layers bank= vegetation and rocks Right Deciduous forest with thick herb and shrub layers bank=vegetation soil and rocks Indicate the dominant vegetation type(s) and record the dominant species
x Trees x Shrubs Grasses Forbs Dominant species Black Walnut White Ash Hackberry and American Sycamore
INSTREAM FEATURES
Measured reach length 100 m Canopy Cover Estimated stream width _35__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area __350_m2
Estimated stream depth __15__m Proportion of Reach Represented by Stream High water mark __22___m Morphology Types Surface velocity (at thalweg) _035__msec x Riffle__30___ x Run__30___ x Pool__40___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___10____ m2 Density of LWD _______ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __30___ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present__________________________________________________________
WATER QUALITY Temperature __18___degC Water Odors x NormalNone Sewage Specific Conductance __-____ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ____-____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used _____NA____ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other___None_____ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach SubstrateType Characteristic
Composition in Sampling Reach
Bedrock 3 Detritus sticks wood coarse plant materials
2 Boulder gt256 mm (10rsquorsquo) 5
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 70 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 22 Sand 006-2 mm (gritty) 0 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiattrsquos Run LOCATION Rt 664 Bridge STATION HLR-3 STREAM CLASS LAT N 39 12934 (upstream) LONG W 078 05019 (upstream) LAT N 39 12958 (downstream) LONG W 078 04958 (downstream) FORM COMPILED BY Sean Robertson RIVER BASIN Potomac River INVESTIGATORS SRobertson BMillholland WBousquet MDahl JPangle
DATE 28 May 2008 TIME 1127 AM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality 2008
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature __14___degC showers (intermittent) x Trace Comments _______________________
_65 x cloud cover ___ ______________________________
x clearsunny __________________________________
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x (Significant springs andor seeps) x (Valley) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS
g
h
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence Some evidence x FieldPasture Industrial x Obvious sources Agricultural x Other Old Package Plant Downstream x Residential Bridge and Road Power line and Pump station Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left 70 field 30 shrubs and deciduous trees Bank=Soil and vegetation Right Maturing deciduous forest steep slope Bank=Shale Bedrock Indicate the dominant vegetation type(s) and record the dominant species
x Trees x Shrubs x Grasses Forbs Dominant species Sycamore and Red Oaks
INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width __51___m Fully open x Partly open Partly shaded Fully shaded Sampling reach area _510_m2
Estimated stream depth __135___m Proportion of Reach Represented by Stream High water mark __-___m Morphology Types Surface velocity (at thalweg) __20___msec x Riffle_40_ x Run_50_ x Pool 10 Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___0____ m2 Density of LWD ___-____ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation ___50____ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present Brown Algae
WATER QUALITY Temperature __17___degC Water Odors x NormalNone Sewage Specific Conductance ___-___ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ___-_____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used ____NA_______ Clear x Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other____None____ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 15 Detritus sticks wood coarse plant materials
0 Boulder gt256 mm (10rsquorsquo) 15
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 62 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 8 Sand 006-2 mm (gritty) 0 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Unnamed Tributary STATION HLR-4 STREAM CLASS LAT N 39 13035 (upstream) LONG W 078 05129 (upstream) LAT N 39 13000 (downstream) LONG W 078 05086 (downstream) FORM COMPILED BY Marie Dahl RIVER BASIN Potomac River INVESTIGATORS M Dahl HKepple SRobertson AFisher
DATE 31 May 2008 TIME 5 PM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality 2008
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) YES x NO
rain (steady rain) Air Temperature __22___degC showers (intermittent) Comments _______________________
_0__ cloud cover _0__ ______________________________
x clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area __-___ km2 mi2 Montane Significant springs andor seeps x Valley Marsh or swamp Montane amp Valley Mixture of origins Other ___________________
COMMENTS Snowden Bridge development (upstream) and sewer line
i
j
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence x FieldPasture Industrial Obvious sources Agricultural Other ____________ Construction upstream and adjacent x Residential Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left Forested with rocky banks field across silt fence and 2m tree strip Right Forested with some rocky banks and forest through the highway Indicate the dominant vegetation type(s) and record the dominant species
x Trees x Shrubs x Grasses Forbs Dominant species White Ash and Hackberry
INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width ___38__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area __380__m2
Estimated stream depth __12__cm Proportion of Reach Represented by Stream High water mark __2___m Morphology Types Surface velocity (at thalweg) __46___msec x Riffle__25___ x Run__70___ x Pool__5___ Channelized Yes x No Dam Present x Yes No
LARGE WOODY DEBRIS
LWD _______ m2 Density of LWD _______ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation ___NA____ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present___Algae___
WATER QUALITY Temperature __16___degC Water Odors x NormalNone Sewage Specific Conductance __-____ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ___-_____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used ____NA_____ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells x Other___Shale_______ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 0 Detritus sticks wood coarse plant materials
10 Boulder gt256 mm (10rsquorsquo) 5
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 45 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 30 Sand 006-2 mm (gritty) 0 Marl grey shell
fragments 0
Silt 0004-006 mm 20 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Lick Run STATION HLR-5 STREAM CLASS LAT N 39 127517 (upstream) LONG W 078 05685(upstream) LAT N 39 12784 (downstream) LONG W 078 05631 (downstream) FORM COMPILED BY B Millholland RIVER BASIN Potomac River INVESTIGATORS B Millholland JTovar SRobertson MDahl WBousquet JPangle
DATE 29 May 2008 TIME 1040 AM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project
k
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature __15___degC showers (intermittent) Comments _______________________
_5_ X cloud cover _0__ ______________________________
x clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane Significant springs andor seeps x Valley Marsh or swamp Montane amp Valley Mixture of origins Other ___________________
COMMENTS Lick Run appears to be intermittent and may be dry in Fall 2008
l
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence x FieldPasture Industrial Obvious sources Agricultural Other ____________ Adjacent logging stream bank scour Residential Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left 85 young deciduous forest 15 logged Bank=Soil vegetation and cobbles Right Young deciduous forest on 30 slope Bank= Soil vegetation and cobble Indicate the dominant vegetation type(s) and record the dominant species
x Trees Shrubs Grasses Forbs Dominant species Mixed Deciduous INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width __11__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area 110 m2
Estimated stream depth _009__m Proportion of Reach Represented by Stream High water mark __96___m Morphology Types Surface velocity (at thalweg) __01___msec x Riffle__30___ x Run__5___ x Pool__65___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___0____ m2 Density of LWD ___-____ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __70___ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present__________________________________________________________
WATER QUALITY Temperature __14___degC Water Odors x NormalNone Sewage Specific Conductance __-__ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ___-___ Water Surface Oils Slick Sheen Globs Flecks pH __-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used _____NA______ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other_____None_____
SEDIMENT AND SUBSTRATE
Odors Deposits Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other________________ x Other Slightly Salty Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 0 Detritus sticks wood coarse plant materials
5 Boulder gt256 mm (10rsquorsquo) 0
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 75 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 23 Sand 006-2 mm (gritty) 2 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION McCann Farm STATION HLR-6 STREAM CLASS LAT N 39 13076 (upstream) LONG W 078 06539 (upstream) LAT N 39 13063 (downstream) LONG W 078 06473 (downstream) FORM COMPILED BY MDahl RIVER BASIN Potomac River INVESTIGATORS MDahl JPangle SRobertson W Bousqet BMillholland JTovar H Kepple
DATE 30 May 2008 TIME 1050 AM (start)
PROJECT NAME
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) YES x NO
rain (steady rain) Air Temperature __18___degC showers (intermittent) Comments _______________________
_85_x cloud cover _10__ ______________________________
clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x (Significant springs andor seeps) x ( Valley) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS Multi-habitat sampling with D-frame nets and seine
m
n
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial x No evidence Some evidence x FieldPasture Industrial Obvious sources x Agricultural Other ____________ Minor stream bank scour Residential Local Watershed Erosion x None Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left 35 bank grasses scattered trees and some bushes Right High bank with grasses and scattered trees Indicate the dominant vegetation type(s) and record the dominant species
x Trees Shrubs Grasses Forbs Dominant species Black Walnut and Festuca elatior
INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width __2___m Fully open Partly open x Partly shaded Fully shaded Sampling reach area __200_m2
Estimated stream depth __03_m Proportion of Reach Represented by Stream High water mark __1___m Morphology Types Surface velocity (at thalweg) __166_msec x Riffle__0___ x Run__55___ x Pool__45___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___2____ m2 Density of LWD ___-____ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __20_____ x Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present ____Glyceria (grass)sp__________
WATER QUALITY Temperature __16___degC Water Odors x NormalNone Sewage Specific Conductance ___-___ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ____-____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used ______NA___ Clear x Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other__NONE__ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 0 Detritus sticks wood coarse plant materials
5 Boulder gt256 mm (10rsquorsquo) 0
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 27 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 38 Sand 006-2 mm (gritty) 5 Marl grey shell
fragments 0 (lt1)
Silt 0004-006 mm 25 Clay lt 0004 mm (slick) 5 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Appendix B
WATER QUALITY ANALYSIS THROUGH THE VIRGINIA
STREAM CONDITION INDEX (VSCI)
The Virginia Stream Condition Index (VSCI) relies on eight metrics to categorize stream health According to Burton and Gerritsen (2003 p 1-1) this index ldquohellip can be used as a primary indicator of ecosystem health and can identify impairment with respect to the reference (or natural) conditionrdquo These metrics are numeric measures of a biological communityrsquos species composition diversity ecological niches or tolerance to pollution
Table 13 on the next page describes each VSCI metric lists its formula and provides the best standard value (BSV) for that metric Based on a 100-point scale the BSV is the score that reflects optimum conditions for aquatic life ie the best water quality value possible (DEQ 2006b pp 8 33) The table also explains whether the score increases or decreases with environmental stress
As Table 13 indicates each of the eight VSCI metrics is calculated individually Each formula provides a unitless score based on a 100-point scale with 0 as the worst possible score and 100 as the best Any metric that provides a result above 100 is changed to 100 in order for the index to work properly After the eight metrics are calculated they are summed and averaged to provide a final VSCI score on a 100-point scale
Based on statistical analysis of Virginiarsquos streams and their BMI communities the DEQ (2006b p 8) currently uses the following tiers to interpret VSCI scores
VSCI Scores Aquatic Life Use
(ALI) Tiers
lt 42 severely stressed
42-55 moderately stressed
55-63 unknownfair (gray zone)
63-73 good
gt73 excellent
Section 2 of this report presents the results of calculations for the six BMI sampling sites the research team studied in the Hiatt-Lick Run subwatershed in 2008
o
p
1 Total taxa the number of distinct taxa in the sample measures the overall diversity of the macroinvertebrate assemblage The best standard value (BSV) is 189 Score = 100 x (X189) Total taxa decreases with stress 2 EPT taxa the sum of the distinct taxa in the orders Ephemeroptera (mayflies) Plecoptera (stoneflies) and Trichoptera (caddisflies) These orders are known to be sensitive to pollution The best standard value (BSV) is 117 Score = 100 x (X117) EPT taxa decreases with stress 3 Percent Ephemeroptera the percent abundance of individuals in the sample that belong to the order Ephemeroptera (mayflies) The best standard value (BSV) is 477 Score = 100 x (X477) Percent Ephemeroptera decreases with stress 4 Percent Plecoptera + Trichoptera less Hydropsychidae the percent abundance of individuals in the sample that belong to the orders Plecoptera (stoneflies) and Trichoptera (caddisflies) excluding the pollution-tolerant caddisfly family Hydropsychidae The best standard value (BSV) is 562 Score = 100 x (X562) Percent Plecoptera + Trichoptera less Hydropsychidae decreases with stress 5 Percent Scrapers the percent abundance of individuals in the sample whose primary functional mechanism for feeding is grazing on the substrate or periphyton (ie attached algae and associated material) The best standard value (BSV) is 467 Score = 100 x (X467) Percent scrapers decreases with stress 6 Percent Chironomidae the percent abundance of individuals in the sample that are Chironomidae (non-biting midge) larvae and pupae The best standard value (BSV) is 16 Score = 100 x [(100-X)(100-16)] Percent Chironomidae increases with stress 7 Percent Top Two Dominant the percent abundance of the two most frequent taxa present in the sample The best standard value (BSV) is 328 Score = 100 x [(100-X)(100-328)] The Percent Top 2 Dominant increases with stress 8 Hilsenhoff Biotic Index (HBI) an abundance-weighted average pollution tolerance of all the individuals within a sample keyed to the family level Tolerance ratings range from 1 (highly intolerant) to 10 (highly tolerant) The best standard value (BSV) is 29 Score = 100 x [(10-X)(10-29)] The Hilsenhoff Biotic Index (HBI) increases with stress ______________________________________________________________________________ Table 13 Description of Benthic Macroinvertebrate VSCI Metrics Includes responses (increases decreases) of each VSCI metric to degraded water quality conditions Adapted from Burton and Gerritsen (2003 pp 3-15 3-16 6-2) and DEQ (2008)
q
Appendix C
LABORATORY BENCH SHEETS
Shenandoah Universityrsquos Environmental Studies Department developed the Benthic Macroinvertebrate Laboratory Bench Sheet and VSCI Worksheet to record BMI specimen identifications and their VSCI classifications for each sample
This form is based in part on bench sheets developed by the EPA and Virginia DEQ for recording specimen identifications Additional columns on Shenandoah Universityrsquos forms for relative numbers and VSCI classifications make the calculation and verification of VSCI scores easier
Note the column for taxonomistrsquos initials Specimens were identified by students in Shenandoah Universityrsquos research group and then verified or where necessary changed by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality This external review helps assure the validity of the study Originals of these bench sheets with handwritten taxonomistsrsquo initials will be kept on file at Shenandoah Universityrsquos Environmental Studies Department for five years after the completion of this study
r
s
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-1 STATION NAME Mouth LAT N 39deg 12749rsquo (upstream) LONG W 078deg 04209rsquo (upstream) LAT N 39deg 12729rsquo (downstream) LONG W 078deg 04148rsquo (downstream) FORM COMPILED BY Jeremy Tovar W S Bousquet RIVER BASIN Potomac River INVESTIGATORS Marie Dahl Brandon Millholand J W Pangle Sean Robertson Jeremy Tovar Woodward Bousquet Ben Bousquet
Collection date 21 May 2008 Sheet completion date 12 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Hirudinea 3 33 JT BVW PR 8 SP Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Coenagrionidae 2 22 BVW PR 9 CR Ephemeroptera (mayflies) Caenidae 1 11 JT BVW CG 6 SP Ephemerllidae 4 44 JT BVW CG 2 CR Heptageniidae 5 55 JT BVW SC 3 CG Isonychiidae 1 11 BVW CF 3 CG Plecoptera (stoneflies) Perlidae 14 154 JT BVW PR 2 CR Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Corydalidae 1 11 JT BVW PR 5 CR Coleoptera (beetles) Elmidae 24 264 I JT BVW SC 4 CG Dytiscidae 4 44 JT BVW PR 6 GN Psephenidae 3 33 JT BVW SC 4 CG Diptera (true flies) Tipulidae 3 33 JT BVW SH 4 BU Chironomidae 26 286 JT BVW CG 6 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
t
Other Total Individuals 91 1001 Total Taxa 13
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
u
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-1 STATION NAME Mouth LAT N 39deg 12749rsquo (upstream) LONG W 078deg 04209rsquo (upstream) LAT N 39deg 12729rsquo (downstream) LONG W 078deg 04148rsquo (downstream) FORM COMPILED BY Jeremy Tovar W S Bousquet RIVER BASIN Potomac River INVESTIGATORS Brandon Millholand JW Pangle Jeremy Tovar Heather Kepple
Collection date 10 October 2008 Sheet completion date 12 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Oligochaeta 3 35 JT BVW CG 8 BU Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Coenagrionidae 2 23 JT BVW PR 9 CR Ephemeroptera (mayflies) Heptageniidae 12 140 JT BVW SC 3 CG Isonychiidae 2 23 BVW CF 3 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Hydropsychidae 16 186 JT BVW CF 6 CG Philopotamidae 31 360 JT BVW CF 2 CG Hemiptera (true bugs) (Veliidae) (5) - JT BVW ( ) Megaloptera (dobsonflies etc) Corydalidae 10 116 JT BVW PR 5 CR Coleoptera (beetles) Dytiscidae 1 12 JT BVW PR 6 GN Psephenidae 7 81 JT BVW SC 4 CG Diptera (true flies) Tipulidae 2 23 BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
v
Other Total Individuals 86
(91) 999
Total Taxa 10 (11)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009 Veliidae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
w
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-2 STATION NAME Package Plant LAT N 39deg 12958rsquo (upstream) LONG W 078deg 04900 (upstream) LAT N 39deg 12958rsquo (downstream) LONG W 078deg 04822 (downstream) FORM COMPILED BY JW Pangle W Bousquet RIVER BASIN Potomac River INVESTIGATORS JW Pangle S Robertson J Tovar M Dahl B Millholland W Bousquet
Collection date 29 May 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Plecoptera (stoneflies) Perlidae 11 136 I JP BVW PR 1 CR Trichoptera (caddisflies) Hydropsychidae 10 123 I JP BVW CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 14 173 I JP BVW SC 4 CG Diptera (true flies) Chironomidae 32 395 I JP BVW CG 6 BU Simuliidae 14 173 I JP BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
x
Other Total Individuals 81 1000 Total Taxa 5
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-2 STATION NAME Package Plant LAT N 39deg 12958rsquo (upstream) LONG W 078deg 04900 (upstream) LAT N 39deg 12958rsquo (downstream) LONG W 078deg 04822 (downstream) FORM COMPILED BY JW Pangle W Bousquet RIVER BASIN Potomac River INVESTIGATORS JW Pangle Brandon Millholland Jeremy Tovar Heather Kepple
Collection date 10 October 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Heptageniidae 40 392 I WBJP SC 3 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Hydropsychidae 7 69 I JP WB BVW CF 6 CG Philopotamidae 28 275 I JP WB BVW CF 2 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Corydalidae 6 59 I JP WB BVW PR 5 CR Coleoptera (beetles) Psephenidae 7 69 I JP WB BVW SC 4 CG Elmidae 8 78 I JP WB BVW SC 4 CG Diptera (true flies) Tipulidae 6 59 I JP WB BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
y
z
Other Total Individuals 102 1001 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-3 STATION NAME Rt 664 Bridge LAT N 39deg 12934rsquo (upstream) LONG W 078deg 05019rsquo (upstream) LAT N 39deg 12951rsquo (downstream) LONG W 078deg 04958rsquo (downstream) FORM COMPILED BY S Robertson W Bousquet RIVER BASIN Potomac River INVESTIGATORS SRobertson BMillholland WBousquet MDahl JPangle
Collection date 28 May 2008 Sheet completion date 11 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Leptophlebiidae 1 15 I SGR BVW CG 2 CR Baetidae 1 15 I BVW CG 5 CG Plecoptera (stoneflies) Perlidae 3 46 I SGR BVW PR 1 CR Nemouridae 1 15 I BVW SH 3 CR Trichoptera (caddisflies) Hydropsychidae 5 77 I SGR BVW CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 27 415 I SGR BVW SC 4 CG Psephenidae 2 31 A SGR BVW SC 4 CG Diptera (true flies) Simuliidae 5 77 I SGR BVW CF 6 CG Chironomidae 20 308 I SGR BVW CG 6 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
aa
bb
Other Total Individuals 65 999 Total Taxa 9
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-3 STATION NAME Rt 664 Bridge LAT N 39deg 12934rsquo (upstream) LONG W 078deg 05019rsquo (upstream) LAT N 39deg 12951rsquo (downstream) LONG W 078deg 04958rsquo (downstream) FORM COMPILED BY S Robertson RIVER BASIN Potomac River INVESTIGATORS S Robertson M Dahl A Fisher H Kepple
Collection date 10 Oct 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Heptageniidae 2 20 I SGR SC 3 CG Isonychiidae 2 20 I BVW CF 2 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Philopotamidae 36 367 I SGR CF 2 CG Hydropsychidae 47 480 I SGR CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 2 20 A SGR SC 4 CG Psephenidae 8 82 A SGR SC 4 CG Diptera (true flies) Tabanidae 1 10 I SGR PR 7 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
cc
dd
Other Total Individuals 98 999 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-4 STATION NAME Unnamed Tributary LAT (upstream) N 39deg 13035rsquo LONG (upstream) W 078deg 05129rsquo LAT (downstream) N 39deg 13000rsquo LONG (downstream) W 078deg 5086rsquo FORM COMPILED BY A Fisher M Dahl rev W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson A Fisher H Kepple
Collection date 31 May 2008 Sheet completion date rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Flatworms (Turbellaria) Worms (Oligochaeta) Naididae 1 14 - BVW CG 8 BU Leeches (Hirudinea) Snails (Gastropoda) Clams (Bivalvia) Isopods (Isopoda) Asellidae 3 42 A AF BVW CG 8 SP Scuds (Amphipoda) Crayfish Shrimp (Decapoda) Water Mites (Hydracarina) Dragonflies (Odonata Anisoptera) Damselflies (Odonata Zygoptera) Mayflies (Ephemeroptera) Stoneflies (Plecoptera) Perlidae 2 28 I AF BVW PR 1 CR Caddisflies (Trichoptera) True Bugs (Hemiptera) Corixidae 1 14 I AF BVW MP 9 DV Dobsonflies (Megaloptera) Beetles (Coleoptera) Dytiscidae 1 14 I AF BVW PR 6 GN True Flies (Diptera) Chironomidae 61 847 I AF BVW CG 6 BU Simuliidae 3 42 I AF BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
ee
ff
Other Total Individuals 72 1001 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-4 STATION NAME Unnamed Tributary LAT (upstream) N 39deg 13035rsquo LONG (upstream) W 078deg 05129rsquo LAT (downstream) N 39deg 13000rsquo LONG (downstream) W 078deg 5086rsquo FORM COMPILED BY Andrew G M Fisher W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson A Fisher H Kepple
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Flatworms (Turbellaria) Worms (Oligochaeta) Lumbriculidae 2 32 A AF BVW CG 8 BU Leeches (Hirudinea) Snails (Gastropoda) Lymnaeidae 1 16 A BVW CG 9 GN Clams (Bivalvia) Isopods (Isopoda) Scuds (Amphipoda) Crayfish Shrimp (Decapoda) Cambaridae 1 16 A AF BVW GN 5 GN Water Mites (Hydracarina) Dragonflies (Odonata Anisoptera) Damselflies (Odonata Zygoptera) Calopterygidae 4 63 I BVW PR 7 CL Mayflies (Ephemeroptera) Tricorythidae 1 16 I BVW CG 5 SP Stoneflies (Plecoptera) Caddisflies (Trichoptera) Phryganeidae 4 63 I AF BVW SH 4 CR Hydropsychidae 6 95 I AF BVW CF 6 CG I True Bugs (Hemiptera) Corixidae 8 127 I AF BVW MP 9 DV (Veliidae ) (7) - I AF BVW ( ) Dobsonflies (Megaloptera) Sialidae 3 48 I AF BVW PR 7 SP Corydalidae 2 32 I AF BVW PR 5 CR Beetles (Coleoptera) Haliplidae 1 16 A AF BVW MP 8 GN Dysticidae 7 111 I AF BVW PR 6 GN Hydrophilidae 1 16 A AF BVW PR 8 GN Elmidae 3 48 I BVW SC 4 CG True Flies (Diptera) Chironomidae 8 127 I AF BVW CG 6 BU Simuliidae 7 111 I AF BVW CF 6 CG Tipulidae 4 63 I AF BVW SH 4 GU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
gg
hh
Other Total Individuals 63
(70) 1000
Total Taxa 17 (18)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Veliidae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-5 STATION NAME Lick Run LAT N 39deg 12752rsquo (upstream) LONG W 078deg 05685rsquo (upstream) LAT N 39deg 12784rsquo (downstream) LONG W 078deg 05631rsquo (downstream) FORM COMPILED BY Brandon Millholland W Bousquet RIVER BASIN Potomac River INVESTIGATORS J Pangle S Robertson W Bousquet JTovar M Dahl
Collection date 29 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Gomphidae 1 15 I BVW PR 3 BU Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Leptophlebiidae 11 167 A LBM BVW CG 2 CR Plecoptera (stoneflies) Perlidae 10 152 A LBM BVW PR 1 CR Perlodiae 1 15 A LBM BVW PR 2 CR Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 5 76 A LBM BVW SC 4 CG Elmidae 2 30 I LBM BVW SC 4 CG Diptera (true flies) (Culicidae) (3) - P LMB BVW ( ) Chironomidae 23 348 I LBM BVW CG 6 BU Simuliidae 13 197 I LBM BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
ii
jj
Other Total Individuals 66
(69) 1000
Total Taxa 7 (8)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Culicidae (Diptera) pupae not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl J Pangle S Robertson W Bousquet B Millholland J Tovar H Kepple
Collection date 30 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 1 12 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Asellidae 114 965 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Plecoptera (stoneflies) Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 2 23 A MRD BVW SC 4 CG Diptera (true flies)
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
kk
ll
Other Total Individuals 117 1000 Total Taxa 3
Notes Subsample taken 29 October 2009 All taxonomic determinations by MRD were confirmed by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson K McCann A Fisher H Kepple A Barrett
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 2 17 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Sphaeriidae 2 17 I MRD BVW CF 8 BU Isopoda (isopods) Asellidae 99 853 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Calopterygidae 2 17 A MRD BVW PR 7 CL Ephemeroptera (mayflies) Leptophlebiidae 2 17 I MRD BVW CG 2 CR Heptageniidae 4 34 A MRD BVW SC 3 CG Plecoptera (stoneflies) Perlidae 1 09 A MRD BVW PR 1 CR Trichoptera (caddisflies) Hemiptera (true bugs) (Gerridae) (1) - A MRD BVW ( ) Corixidae 1 09 A MRD BVW PR 5 CR Megaloptera (dobsonflies etc) Coleoptera (beetles) Dryopidae 1 09 A MRD BVW SC 5 CG Diptera (true flies) Tipulidae 2 17 I BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
mm
nn
Other Total Individuals 116
(117) 999
Total Taxa 10 (11)
Notes Subsample taken 6 November 2008 Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009 Gerridae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
- Disclaimer ii
- Title Page iii
- Acknowledgements v
- Table of Contents vii
- SECTION 2 RESULTS DATA TABLES 7
- OVERVIEW AND FINDINGS
- Introduction
- RESULTS DATA TABLES
- REFERENCES
- FIELD DATA SHEETS
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- SubstrateType
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- WATER QUALITY ANALYSIS
- THROUGH THE VIRGINIA
- STREAM CONDITION INDEX (VSCI)
- LABORATORY BENCH SHEETS
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
TABLE OF CONTENTS page
Disclaimer ii Title Page iii Acknowledgements v Table of Contents vii SECTION 1 OVERVIEW AND FINDINGS 1 Introduction 1 Methods and Analysis 3 Findings 3 Future Research 5 SECTION 2 RESULTS DATA TABLES 7 Site HLR-1 Mouth 7 Site HLR-2 Package Plant 8 Site HLR-3 Route 664 Bridge 9 Site HLR-4 Unnamed Tributary 10 Site HLR-5 Lick Run 11 Site HLR-6 McCann Farm 12 REFERENCES 13 APPENDICES 15 Appendix A Field Data Sheets Appendix B Water Quality Analysis through the Virginia Stream Condition Index (VSCI) Appendix C Laboratory Bench Sheets Note Photographs of the six sampling sites appear on a CD provided separately to the grant administrator
vii
viii
Section 1
OVERVIEW AND FINDINGS
Introduction
This report presents baseline water quality data for Hiatt Run and Lick Run -- termed the Hiatt-Lick Run subwatershed in this study Located in Frederick County Virginia Hiatt and Lick Runs are tributaries of Opequon Creek The Opequon in turn empties into the Potomac River and then the Chesapeake Bay The investigation was conducted by a Shenandoah University research team in 2008 Researchers used benthic macroinvertebrates (BMIs) as biological indicators of water quality This report presents an overview of the study followed by summary tables and a list of references The Appendix contains data sheets and a description of the data analysis These data will form part of the baseline water quality assessment that is being undertaken through the Opequon Creek Targeted Watershed Grant The grant administered by Jim Lawrence reflects a broad-based partnership that includes Virginia Tech Shenandoah University the Frederick-Winchester Service Authority The Opequon Watershed (TOW) citizens organization as well as federal state and local governments community groups and business interests Grant partners will use best management practices (BMPs) to reduce nutrient runoff and address other pollution problems in the Opequon Creek watershed Since 1996 Opequon Creek has appeared on the impaired waters list prepared by the Virginia Department of Environmental Quality (DEQ) The Hiatt-Lick Run subwatershed was selected for this study because it represents changes that are typical of the Opequon Creek watershed These changes include new commercial and residential developments as well as the implementation of water quality improvement practices such as tree planting in riparian zones fences to prevent livestock from entering streams and replacing outdated sewage treatment facilities such as a package plant on lower Hiatt Run
Seven students from Shenandoah Universityrsquos Environmental Studies Department conducted this study from May through December 2008 under the direction of Woodward Bousquet Professor of Environmental Studies and Biology The research team followed procedures described in the Rapid Bioassessment Protocols (RBPs) of the US Environmental Protection Agency (Barbour et al 1999) and the Virginia Stream Condition Index (VSCI) The VSCI provides an evaluation of water quality based on a streamrsquos benthic macroinvertebrate community (Burton and Gerritsen 2003 DEQ 2006b and 2008)
In consultation with Billy van Wart of the DEQ and grant administrator Jim Lawrence the Shenandoah University researchers selected six representative sampling sites in the Hiatt-Lick Run subwatershed Each site was given a name and an HLR (for Hiatt-Lick Run) code Figure 1 maps their positions while their characteristics and coordinates appear in the field data sheets (see Appendix A) These sites were established according to EPA and DEQ guidelines that generally call for a 100-meter-long stream reach with at least three riffle zones a width sufficient to accommodate a 1-meter seine and a substrate composed of least 35 cobbles
Specific locations of the sampling sites on Hiatt and Lick Runs were selected to reflect different watershed positions and changing land uses See Figure 1 Further information about each sampling site appears in the field data sheets (Appendix A)
1
Figure 1 Sampling Sites in the Hiatt-Lick Run Subwatershed of Opequon Creek Frederick County Virginia Scale 1 centimeter = 500 meters
HLR-1 (Mouth) measures water quality at the lowest point of the subwatershed as Hiatt Run flows into Opequon Creek Forests cover the immediate riparian zones on both sides of Hiatt Run This site will be used in future years by the DEQ for BMI monitoring HLR-2 (Package Plant) is situated on Hiatt Run just below the outflow pipe of an outdated package plant that formerly treated sewage from the Jordan Springs area During the study period the package plant was taken off-line and a pumping station was constructed The Frederick-Winchester Service Authority now treats this sewage at its Opequon Water Reclamation Facility HLR-3 (Route 664 Bridge) was chosen because it is located upstream from the package plant but downstream of the confluence of Hiatt Run and Lick Run HLR-4 (Unnamed Tributary) is located downstream of the Snowden Bridge residential development which has been under construction The area immediately upstream of this sampling site was being graded by heavy equipment during the May 2008 sampling time HLR-5 (Lick Run) the researchers learned dries up in late summer It had a modest but constant flow during when the May 2008 sample was taken HLR-6 (McCann Farm) represents the headwater areas of the Hiatt-Lick Run subwatershed of Opequon Creek The McCanns who graze cattle in their pastures that surround Hiatt Run here have been working with the Potomac Conservancy to implement Best Management Practices
2
(BMPs) to improve water quality These practices include fencing cattle out of the stream and spring runs and planting more trees in the riparian zone
Methods and Analysis
The research team established a sampling plan and then sampled according to methods the DEQ developed for the VSCI Using a 1 meter x 1 meter seine samples from 2m2 of substrate in riffle zones were to be collected twice at each site once in May 2008 and once in October 2008 At five of the six sites stream characteristics permitted the standard single-habitat sampling procedure At the McCann Farm (Site HLR-6) researchers needed to use multi-habitat sampling See Barbour et al (1999 pp 7-3 ff) for further descriptions of these two methods
All six sites were sampled in May 2008 Low-water conditions in October 2008 prevented the Shenandoah University group from collecting BMIs at Lick Run (HLR-5) that month but the other five sites were sampled successfully As a result the research team obtained eleven samples for analysis in this study Samples were preserved in ethanol for subsequent identification
DEQ procedures require subsampling when the number of BMIs in a given sample exceeds 100 All eleven samples in this study met that criterion Consequently researchers used a Caton tray to produce random subsamples of 90 to 110 specimens from each sample During the analysis that followed some specimens in the subsamples were rejected either because they turned out to be terrestrial species or because they were in the wrong life stage (eg pupae) In most cases the researchers identified specimens to the family level Primary taxonomic references were A Guide to Common Freshwater Invertebrates of North America (Voshell 2002) Fresh-Water Invertebrates of the United States (Pennak 1953) and An Introduction to the Aquatic Insects of North America (Merritt and Cummins 1996) DEQ Regional Aquatic Biologist Billy van Wart reviewed verified and where necessary corrected the identifications
The investigators then used the Virginia Stream Condition Index (VSCI) to produce water quality scores based on the BMIs collected A VSCI score is based on eight different measurement formulas called metrics Each metric is a numeric measure of a biological communityrsquos species composition diversity ecological niches or tolerance to pollution When combined into a single multimetric VSCI score the response of the benthic community to water conditions can be evaluated For further information and worksheets see Appendices B and C respectively
Findings
Table 1 presents the VSCI scores for the eleven samples collected in the Hiatt-Lick Run subwatershed in 2008 Since a sample year consists of two samples the mean scores for each site for the entire year are also included As the table shows
bull None of the 11 samples was rated excellent bull Only 1 of the 11 samples was rated as good bull Only 2 of the 11 samples were rated fair (ie in the ldquogrey zonerdquo between impaired and
unimpaired) bull The remaining 8 samples were in either moderately stressed or severely stressed
condition
3
Site Name and Code Sampling
Date of
TaxaVCSI Score
Water Quality Rating
Mouth (HLR-1) 21 May 2008 13 571 Fair (Gray Zone)
Mouth (HLR-1) 10 Oct 2008 10 608 Fair (Gray Zone)
site average for 2008 590 Fair (Gray Zone)
Package Plant (HLR-2) 29 May 2008 5 370 Severely Stressed
Package Plant (HLR-2) 10 Oct 2008 7 674 Good
site average for 2008 522 Moderately Stressed
Route 664 Bridge (HLR-3) 28 May 2008 9 485 Moderately Stressed
Route 664 Bridge (HLR-3) 10 Oct 2008 7 469 Moderately Stressed
site average for 2008 477 Moderately Stressed
Unnamed Tributary (HLR-4) 31 May 2008 7 173 Severely Stressed
Unnamed Tributary (HLR-4) 10 Oct 2008 17 476 Moderately Stressed
site average for 2008 325 Severely Stressed
Upper Lick Run (HLR-5) 29 May 2008 7 455 Moderately Stressed
site average for 2008 455 Moderately Stressed
McCann Farm (HLR-6) 30 May 2008 3 190 Severely Stressed
McCann Farm (HLR-6) 10 Oct 2008 10 316 Severely Stressed
site average for 2008 253 Severely Stressed
Summary 6 sites 11 samples
36 BMI taxa
VSCI = Virginia Stream Condition Index see Burton and Gerritsen (2003) and Virginia Department of Environmental Quality (2006b and 2008) Table 1 Summary Table of Virginia Stream Condition Index (VSCI) Scores and Water Quality Ratings for Sites in the Hiatt-Lick Run Subwatershed of Opequon Creek Frederick County Virginia 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
4
Improvements in water quality ratings at three sites between May and October suggest that BMPs and other changes may already be having some positive effects on water quality At the Package Plant (Site HLR-2) the VSCI score rose dramatically from 370 (severely stressed) to 674 (good) The small-scale sewage treatment plant as noted above was taken off-line during this period Similarly water quality at the Unnamed Tributary (HLR-4) increased from 173 (severely stressed) to 476 (moderately stressed) Adjacent construction was completed over the summer and grass was planted on the property Management practices on the McCann Farm (HLR-6) may be responsible for improving conditions for aquatic life in Hiatt Runrsquos headwaters Even though the water quality was still rated as severely stressed in October the VSCI score rose from 190 to 316 at this sampling site Further studies are needed to determine whether the changes noted in 2008 persist into the future In 2003 the DEQ began a Total Maximum Daily Load (TMDL) process for Opequon Creek as required by the Water Quality Act of 1987 The TMDL approach establishes a ldquopollution budgetrdquo for each impaired stream for each type of impairment and for each responsible pollutant In conjunction with the DEQ and the Virginia Department of Conservation and Recreation (DCR) Virginia Polytechnic Institutersquos Department of Biosystems Engineering developed a TMDL implementation plan (Opequon Creek IP Steering Committee et al 2006) Monitoring the BMI communities of Opequon Creek and its tributaries is essential to evaluating the progress of the proposed water quality improvement projects
Future Research The relatively small Hiatt-Lick Run subwatershed provides several case studies that can help assess the effectiveness of various water quality management practices not only in Opequon Creek but also in other watersheds in the Chesapeake Bay region and beyond In supporting that goal this study makes two principal contributions 1 It provides baseline data to measure the improvement or degradation of the Hiatt-Lick Run section of the Opequon Creek watershed and 2 It establishes a monitoring framework for the long-term biological monitoring of water quality and the evaluation of best management practices (BMPs) This framework consists of
bull Sampling Sites ndash six stream reaches in the Hiatt-Lick Run subwatershed selected as sampling sites according to EPA and DEQ specifications
bull Data Collection Protocols ndash based on the EPArsquos Rapid Bioassessment Protocols (RBPs)
(Barbour et al 1999) and the DEQrsquos Virginia Stream Index (VSCI) (Burton and Gerritsen 2003 DEQ 2006b and 2008) and
bull Analytical Procedures ndash contained in the Virginia Stream Condition Index (VSCI see
Burton and Gerritsen 2003 DEQ 2006b pp 8 and 33 and DEQ 2008 pp 29-34)
5
6
Section 2
RESULTS DATA TABLES
Site HLR-1 Mouth
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 13 189 6872 EPT taxa 5 117 4273 Ephemeroptera 121 477 2544 Plec +Tric less Hydropsych 154 562 2745 Scrapers 352 467 7536 Chironomidae 286 16 7267 Top 2 Dominant 550 328 6708 Hilsenhoff Biotic Index (HBI) 45 29 775VSCI Score 571
According to the DEQrsquos water quality standards aquatic life at this site is fair (gray zone) See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 2 VSCI Scores for Mouth (HLR-1) Site in Hiatt-Lick Run Sampled on 21 May 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 10 189 5292 EPT taxa 4 117 3423 Ephemeroptera 163 477 3424 Plec +Tric less Hydropsych 360 562 6415 Scrapers 221 467 4736 Chironomidae 00 16 10007 Top 2 Dominant 546 328 6768 Hilsenhoff Biotic Index (HBI) 39 29 859VSCI Score 608
According to the DEQrsquos water quality standards aquatic life at this site is fair (gray zone) See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 3 VSCI Scores for Mouth (HLR-1) Site in Hiatt-Lick Run Sampled 10 October 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
7
Site HLR-2 Package Plant
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 5 189 2642 EPT taxa 2 117 1713 Ephemeroptera 00 477 004 Plec +Tric less Hydropsych 110 562 1965 Scrapers 173 467 3706 Chironomidae 395 16 6157 Top 2 Dominant 568 328 6438 Hilsenhoff Biotic Index (HBI) 50 29 704VSCI Score 370
According to the DEQrsquos water quality standards aquatic life at this site is severely stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 4 VSCI Scores for Package Plant (HLR-2) Site in Hiatt-Lick Run Sampled 29 May 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 7 189 3702 EPT taxa 34 117 2563 Ephemeroptera 392 477 8214 Plec +Tric less Hydropsych 275 562 4895 Scrapers 539 467 10006 Chironomidae 00 16 10007 Top 2 Dominant 667 328 4968 Hilsenhoff Biotic Index (HBI) 32 29 958VSCI Score 674
According to the DEQrsquos water quality standards aquatic life at this site is good See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 5 VSCI Scores for Package Plant (HLR-2) Site in Hiatt-Lick Run Sampled 10 October 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
8
Site HLR-3 Route 664 Bridge
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 9 189 4762 EPT taxa 5 117 4273 Ephemeroptera 30 477 634 Plec +Tric less Hydropsych 61 562 1095 Scrapers 446 467 9556 Chironomidae 308 16 7037 Top 2 Dominant 723 328 4128 Hilsenhoff Biotic Index (HBI) 48 29 732VSCI Score 485
According to the DEQrsquos water quality standards aquatic life at this site is moderately stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 6 VSCI Scores for Route 664 Bridge (HLR-3) Site in Hiatt-Lick Run Sampled 28 May 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 7 189 3702 EPT taxa 4 117 3423 Ephemeroptera 40 477 844 Plec +Tric less Hydropsych 367 562 6535 Scrapers 122 467 2616 Chironomidae 00 16 10007 Top 2 Dominant 847 328 2288 Hilsenhoff Biotic Index (HBI) 42 29 817VSCI Score 469
According to the DEQrsquos water quality standards aquatic life at this site is moderately stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 7 VSCI Scores for Route 664 Bridge (HLR-3) Site in Hiatt-Lick Run Sampled 10 October 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
9
Site HLR-4 Unnamed Tributary
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 7 189 3702 EPT taxa 1 117 853 Ephemeroptera 00 477 004 Plec +Tric less Hydropsych 28 562 495 Scrapers 00 467 006 Chironomidae 847 16 1557 Top 2 Dominant 889 328 1658 Hilsenhoff Biotic Index (HBI) 60 29 563VSCI Score 173
According to the DEQrsquos water quality standards aquatic life at this site is severely stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 8 VSCI Scores for Unnamed Tributary (HLR-4) Site in Hiatt-Lick Run Sampled 31 May 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 17 189 8992 EPT taxa 3 117 2563 Ephemeroptera 16 477 344 Plec +Tric less Hydropsych 63 562 1125 Scrapers 48 467 1036 Chironomidae 127 16 8877 Top 2 Dominant 254 328 10008 Hilsenhoff Biotic Index (HBI) 63 29 521VSCI Score 476
According to the DEQrsquos water quality standards aquatic life at this site is moderately stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 9 VSCI Scores for Unnamed Tributary (HLR-4) Site in Hiatt-Lick Run Sampled 10 October 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
10
Site HLR-5 Lick Run
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 7 189 3702 EPT taxa 3 117 2563 Ephemeroptera 167 477 3504 Plec +Tric less Hydropsych 167 562 2975 Scrapers 106 467 2276 Chironomidae 348 16 6637 Top 2 Dominant 545 328 6778 Hilsenhoff Biotic Index (HBI) 43 29 803VSCI Score 455
According to the DEQrsquos water quality standards aquatic life at this site is moderately stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 10 VSCI Scores for Lick Run (HLR-5) Site in Hiatt-Lick Run Sampled 29 May 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
Note
Water conditions at the Lick Run (HLR-5) site were too low in October 2008 to permit a sample to be taken
11
Site HLR-6 McCann Farm
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 3 189 1592 EPT taxa 0 117 003 Ephemeroptera 00 477 004 Plec +Tric less Hydropsych 00 562 005 Scrapers 23 467 496 Chironomidae 00 16 10007 Top 2 Dominant 988 328 188 Hilsenhoff Biotic Index (HBI) 79 29 296VSCI Score 190
According to the DEQrsquos water quality standards aquatic life at this site is severely stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 11 VSCI Scores for McCann Farm (HLR-6) Site in Hiatt-Lick Run Sampled 30 May 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 10 189 5292 EPT taxa 3 117 2563 Ephemeroptera 51 477 1074 Plec +Tric less Hydropsych 09 562 165 Scrapers 43 467 926 Chironomidae 00 16 10007 Top 2 Dominant 887 328 1688 Hilsenhoff Biotic Index (HBI) 75 29 352VSCI Score 315
According to the DEQrsquos water quality standards aquatic life at this site is severely stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 12 VSCI Scores for McCann Farm (HLR-6) Site in Hiatt-Lick Run Sampled 10 October 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
12
REFERENCES
Barbour Michael T Jeroen Gerritsen Blaine D Snyder and James B Stribling 1999 Rapid bioassessment protocols for use in streams and wadeable rivers periphyton benthic macroinvertebrates and fish Second edition EPA 841-B-99-002 Washington DC US Environmental Protection Agency Office of Water Available online at httpwwwepagovOWOWmonitoringbioasspshtml Burton June and Jeroen Gerritsen 2003 A stream condition index for Virginia non-coastal streams Prepared for the US EPA Office of Science and Technology US EPA Region 3 Environmental Services Division and Virginia Department of Environmental Quality Owings Mills Maryland Tetra Tech Inc Available online at httpwwwdeqvirginiagovwatermonitoringpdf vastrmconpdf Hessler Rachel A Andrea R Martin Heidi D Ruggeri William B Seymour Jr and Woodward S Bousquet 2006 Water quality assessment using benthic macroinvertebrates A study and compilation Winchester Virginia Environmental Studies Program Shenandoah University Occasional publication no 10 Igoe Robert 2006 DEQ identifies nine impaired streams in Clarke Frederick The Winchester Star 15 July B1 Jackson Charlie 2005 Survey targets Opequon cleanup The Winchester Star 19 August B1 Johnston Elizabeth A and Woodward S Bousquet 2003 What happens when it rains A study of water quality in Abrams Creek and Town Run Winchester and Frederick County Virginia Winchester Virginia Environmental Studies Program Shenandoah University Occasional publication no7 Merritt Richard W and Kenneth W Cummins 1996 An introduction to the aquatic insects of North America Dubuque Iowa KendallHunt Publishing Company Opequon Creek IP Steering Committee Virginia Tech Department of Biological Systems Engineering and Center for TMDL and Watershed Studies University of Virginia Institute for Environmental Negotiation and West Virginia University Division of Resource Management 2006 Opequon Creek watershed TMDL implementation plan Blacksburg Virginia Virginia Tech Department of Biological Systems Engineering Available online at httpwwwtmdlbsevteduoutreachC82 Pennak Robert W 1953 Fresh-water invertebrates of the United States New York New York The Ronald Press Company Rosenburg David M and Vincent H Resh 1993 Freshwater biomonitoring and benthic macroinvertebrates New York New York Chapman amp Hall Shenandoah University Public Relations Office 2002 Data collected by SU and its partners helps identify impaired water Winchester Virginia Available online at httpwwwsuedutemp_ newscfmurlnum=32 Thorp James H and Alan P Covich 1991 Ecology and classification of North American freshwater invertebrates San Diego California Academic Press Inc
13
US Environmental Protection Agency 1997 Volunteer stream monitoring a methods manual EPA 841-B-97-003 Washington DC US Environmental Protection Agency Office of Water Van Meter Val 2003 Opequon Creek pollution limits to be established The Winchester Star 4 April B1 Virginia Department of Environmental Quality 2006a Virginia 305(b)303(d) water quality integrated report to Congress and the EPA Administrator for the period January 1 2000 to December 31 2004 Richmond Virginia Virginia Department of Environmental Quality Available online at httpwwwdeqvirginiagovwqair2006html __________ 2006b Using probabilistic data to validate the non-coastal Virginia Stream Condition Index (draft) VDEQ technical bulletin WQA2006-001 Virginia Department of Environmental Quality Available online at httpwwwloudounwatershedorgstream_assessmentreference_documents VA_DEQ_SCI_Validationpdf __________ 2008 Biological monitoring program quality assurance project plan for wadeable streams and rivers Richmond Virginia Virginia Department of Environmental Quality Update available online at httpwwwdeqstatevausbiomonitoringdocumentsBioMonQAPP_13Aug2008pdf Virginia Tech Aquatic Entomology Laboratory 2005 Taxa dictionary [Excel file] Blacksburg Virginia Virginia Tech Department of Biological Systems Engineering 2003a Opequon watershed TMDLs for benthic impairments Abrams Creek and Lower Opequon Creek Frederick and Clarke Counties Virginia Prepared for the Virginia Department of Environmental Quality and Virginia Department of Conservation and Recreation Blacksburg Virginia Available online at httpwwwdeqstate vaustmdlapptmdlsshenrvrabropebcpdf Voshell J Reese Jr 2002 A guide to common freshwater invertebrates of North America Blacksburg Virginia The McDonald amp Woodward Publishing Company
14
APPENDICES
15
16
a
Appendix A
FIELD DATA SHEETS
This appendix presents a Site Characterization Field Data Sheet for each of the six sampling reaches established on Hiatt Run and Lick Run for this study They were filled out during the sampling runs made in May 2008
These forms were adapted by Shenandoah University from forms used by the EPA and Virginia DEQ for recording characteristics of sampling sites
b
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Mouth STATION _HLR-1_____ STREAM CLASS LAT N 39 12749 (upstream) LONG W 078 04209(upstream) LAT N 39 12729 (downstream) LONG W 078 04148 (downstream) FORM COMPILED BY Jeremy Tovar RIVER BASIN Potomac River INVESTIGATORS JPangle B Millholland MDahl SRobertson W Bousquet JTovar BBousquet
DATE May 21 2008_________ TIME_250 PM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature __24___degC showers (intermittent) Comments _______________________
___ cloud cover _0_ ______________________________
X clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x ( Significant springs andor seeps) x (Valley ) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS
c
d
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence x FieldPasture Industrial Obvious sources Agricultural Other ____________ Stream scour steep slope erosion x Residential Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left Field and young thin deciduous forest 100 Bank=Grass cobble and shrub Right 90 maturing deciduous forest 45 shale slope Bank=Bedrock soil and herbs Indicate the dominant vegetation type(s) and record the dominant species
x Trees Shrubs x (Grasses) Forbs Dominant species Oak species and mix deciduous
INSTREAM FEATURES
Measured reach length ___100__m Canopy Cover Estimated stream width _35__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area __350_m2
Estimated stream depth _008_m Proportion of Reach Represented by Stream High water mark _15_m Morphology Types Surface velocity (at thalweg) _05__msec x Riffle__75___ x Run__20___ x Pool__5___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___00__ m2 Density of LWD _-_ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __15_____ x Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present Algae
WATER QUALITY Temperature __19___degC Water Odors x NormalNone Sewage Specific Conductance - Petroleum Chemical Fishy Other___________________ Dissolved Oxygen - Water Surface Oils Slick Sheen Globs Flecks pH __-_ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used _____NA________ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic x None Relict shells Other______None_____ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 8 Detritus sticks wood coarse plant materials
0 Boulder gt256 mm (10rsquorsquo) 0
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 51 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 40 Sand 006-2 mm (gritty) 1 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University
SITE CHARACTERIZATION FIELD DATA SHEET FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Package Plant STATION HLR-2 STREAM CLASS LAT N 39 129580 (upstream) LONG W 078 04900 (upstream) LAT N 39 129581 (downstream) LONG W 078 04822 (downstream) FORM COMPILED BY JW Pangle RIVER BASIN Potomac River INVESTIGATORS JPangle JTovar MDahl B Millholland SRobertson W Bousquet
DATE__19 May 2008_ TIME__1240__ PM
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature _____degC showers (intermittent) Comments __Sunny Day_____________
_5__ cloud cover _0__ ______________________________
x clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x (Significant springs andor seeps) x (Valley) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS
e
f
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence FieldPasture Industrial Obvious sources Agricultural Other ____________ __Upstream Construction and Stream bank Scour Residential __________________________________ Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left Deciduous forest with thick herb and shrub layers bank= vegetation and rocks Right Deciduous forest with thick herb and shrub layers bank=vegetation soil and rocks Indicate the dominant vegetation type(s) and record the dominant species
x Trees x Shrubs Grasses Forbs Dominant species Black Walnut White Ash Hackberry and American Sycamore
INSTREAM FEATURES
Measured reach length 100 m Canopy Cover Estimated stream width _35__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area __350_m2
Estimated stream depth __15__m Proportion of Reach Represented by Stream High water mark __22___m Morphology Types Surface velocity (at thalweg) _035__msec x Riffle__30___ x Run__30___ x Pool__40___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___10____ m2 Density of LWD _______ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __30___ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present__________________________________________________________
WATER QUALITY Temperature __18___degC Water Odors x NormalNone Sewage Specific Conductance __-____ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ____-____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used _____NA____ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other___None_____ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach SubstrateType Characteristic
Composition in Sampling Reach
Bedrock 3 Detritus sticks wood coarse plant materials
2 Boulder gt256 mm (10rsquorsquo) 5
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 70 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 22 Sand 006-2 mm (gritty) 0 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiattrsquos Run LOCATION Rt 664 Bridge STATION HLR-3 STREAM CLASS LAT N 39 12934 (upstream) LONG W 078 05019 (upstream) LAT N 39 12958 (downstream) LONG W 078 04958 (downstream) FORM COMPILED BY Sean Robertson RIVER BASIN Potomac River INVESTIGATORS SRobertson BMillholland WBousquet MDahl JPangle
DATE 28 May 2008 TIME 1127 AM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality 2008
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature __14___degC showers (intermittent) x Trace Comments _______________________
_65 x cloud cover ___ ______________________________
x clearsunny __________________________________
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x (Significant springs andor seeps) x (Valley) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS
g
h
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence Some evidence x FieldPasture Industrial x Obvious sources Agricultural x Other Old Package Plant Downstream x Residential Bridge and Road Power line and Pump station Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left 70 field 30 shrubs and deciduous trees Bank=Soil and vegetation Right Maturing deciduous forest steep slope Bank=Shale Bedrock Indicate the dominant vegetation type(s) and record the dominant species
x Trees x Shrubs x Grasses Forbs Dominant species Sycamore and Red Oaks
INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width __51___m Fully open x Partly open Partly shaded Fully shaded Sampling reach area _510_m2
Estimated stream depth __135___m Proportion of Reach Represented by Stream High water mark __-___m Morphology Types Surface velocity (at thalweg) __20___msec x Riffle_40_ x Run_50_ x Pool 10 Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___0____ m2 Density of LWD ___-____ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation ___50____ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present Brown Algae
WATER QUALITY Temperature __17___degC Water Odors x NormalNone Sewage Specific Conductance ___-___ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ___-_____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used ____NA_______ Clear x Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other____None____ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 15 Detritus sticks wood coarse plant materials
0 Boulder gt256 mm (10rsquorsquo) 15
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 62 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 8 Sand 006-2 mm (gritty) 0 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Unnamed Tributary STATION HLR-4 STREAM CLASS LAT N 39 13035 (upstream) LONG W 078 05129 (upstream) LAT N 39 13000 (downstream) LONG W 078 05086 (downstream) FORM COMPILED BY Marie Dahl RIVER BASIN Potomac River INVESTIGATORS M Dahl HKepple SRobertson AFisher
DATE 31 May 2008 TIME 5 PM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality 2008
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) YES x NO
rain (steady rain) Air Temperature __22___degC showers (intermittent) Comments _______________________
_0__ cloud cover _0__ ______________________________
x clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area __-___ km2 mi2 Montane Significant springs andor seeps x Valley Marsh or swamp Montane amp Valley Mixture of origins Other ___________________
COMMENTS Snowden Bridge development (upstream) and sewer line
i
j
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence x FieldPasture Industrial Obvious sources Agricultural Other ____________ Construction upstream and adjacent x Residential Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left Forested with rocky banks field across silt fence and 2m tree strip Right Forested with some rocky banks and forest through the highway Indicate the dominant vegetation type(s) and record the dominant species
x Trees x Shrubs x Grasses Forbs Dominant species White Ash and Hackberry
INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width ___38__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area __380__m2
Estimated stream depth __12__cm Proportion of Reach Represented by Stream High water mark __2___m Morphology Types Surface velocity (at thalweg) __46___msec x Riffle__25___ x Run__70___ x Pool__5___ Channelized Yes x No Dam Present x Yes No
LARGE WOODY DEBRIS
LWD _______ m2 Density of LWD _______ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation ___NA____ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present___Algae___
WATER QUALITY Temperature __16___degC Water Odors x NormalNone Sewage Specific Conductance __-____ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ___-_____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used ____NA_____ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells x Other___Shale_______ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 0 Detritus sticks wood coarse plant materials
10 Boulder gt256 mm (10rsquorsquo) 5
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 45 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 30 Sand 006-2 mm (gritty) 0 Marl grey shell
fragments 0
Silt 0004-006 mm 20 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Lick Run STATION HLR-5 STREAM CLASS LAT N 39 127517 (upstream) LONG W 078 05685(upstream) LAT N 39 12784 (downstream) LONG W 078 05631 (downstream) FORM COMPILED BY B Millholland RIVER BASIN Potomac River INVESTIGATORS B Millholland JTovar SRobertson MDahl WBousquet JPangle
DATE 29 May 2008 TIME 1040 AM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project
k
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature __15___degC showers (intermittent) Comments _______________________
_5_ X cloud cover _0__ ______________________________
x clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane Significant springs andor seeps x Valley Marsh or swamp Montane amp Valley Mixture of origins Other ___________________
COMMENTS Lick Run appears to be intermittent and may be dry in Fall 2008
l
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence x FieldPasture Industrial Obvious sources Agricultural Other ____________ Adjacent logging stream bank scour Residential Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left 85 young deciduous forest 15 logged Bank=Soil vegetation and cobbles Right Young deciduous forest on 30 slope Bank= Soil vegetation and cobble Indicate the dominant vegetation type(s) and record the dominant species
x Trees Shrubs Grasses Forbs Dominant species Mixed Deciduous INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width __11__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area 110 m2
Estimated stream depth _009__m Proportion of Reach Represented by Stream High water mark __96___m Morphology Types Surface velocity (at thalweg) __01___msec x Riffle__30___ x Run__5___ x Pool__65___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___0____ m2 Density of LWD ___-____ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __70___ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present__________________________________________________________
WATER QUALITY Temperature __14___degC Water Odors x NormalNone Sewage Specific Conductance __-__ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ___-___ Water Surface Oils Slick Sheen Globs Flecks pH __-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used _____NA______ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other_____None_____
SEDIMENT AND SUBSTRATE
Odors Deposits Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other________________ x Other Slightly Salty Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 0 Detritus sticks wood coarse plant materials
5 Boulder gt256 mm (10rsquorsquo) 0
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 75 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 23 Sand 006-2 mm (gritty) 2 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION McCann Farm STATION HLR-6 STREAM CLASS LAT N 39 13076 (upstream) LONG W 078 06539 (upstream) LAT N 39 13063 (downstream) LONG W 078 06473 (downstream) FORM COMPILED BY MDahl RIVER BASIN Potomac River INVESTIGATORS MDahl JPangle SRobertson W Bousqet BMillholland JTovar H Kepple
DATE 30 May 2008 TIME 1050 AM (start)
PROJECT NAME
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) YES x NO
rain (steady rain) Air Temperature __18___degC showers (intermittent) Comments _______________________
_85_x cloud cover _10__ ______________________________
clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x (Significant springs andor seeps) x ( Valley) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS Multi-habitat sampling with D-frame nets and seine
m
n
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial x No evidence Some evidence x FieldPasture Industrial Obvious sources x Agricultural Other ____________ Minor stream bank scour Residential Local Watershed Erosion x None Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left 35 bank grasses scattered trees and some bushes Right High bank with grasses and scattered trees Indicate the dominant vegetation type(s) and record the dominant species
x Trees Shrubs Grasses Forbs Dominant species Black Walnut and Festuca elatior
INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width __2___m Fully open Partly open x Partly shaded Fully shaded Sampling reach area __200_m2
Estimated stream depth __03_m Proportion of Reach Represented by Stream High water mark __1___m Morphology Types Surface velocity (at thalweg) __166_msec x Riffle__0___ x Run__55___ x Pool__45___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___2____ m2 Density of LWD ___-____ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __20_____ x Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present ____Glyceria (grass)sp__________
WATER QUALITY Temperature __16___degC Water Odors x NormalNone Sewage Specific Conductance ___-___ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ____-____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used ______NA___ Clear x Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other__NONE__ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 0 Detritus sticks wood coarse plant materials
5 Boulder gt256 mm (10rsquorsquo) 0
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 27 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 38 Sand 006-2 mm (gritty) 5 Marl grey shell
fragments 0 (lt1)
Silt 0004-006 mm 25 Clay lt 0004 mm (slick) 5 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Appendix B
WATER QUALITY ANALYSIS THROUGH THE VIRGINIA
STREAM CONDITION INDEX (VSCI)
The Virginia Stream Condition Index (VSCI) relies on eight metrics to categorize stream health According to Burton and Gerritsen (2003 p 1-1) this index ldquohellip can be used as a primary indicator of ecosystem health and can identify impairment with respect to the reference (or natural) conditionrdquo These metrics are numeric measures of a biological communityrsquos species composition diversity ecological niches or tolerance to pollution
Table 13 on the next page describes each VSCI metric lists its formula and provides the best standard value (BSV) for that metric Based on a 100-point scale the BSV is the score that reflects optimum conditions for aquatic life ie the best water quality value possible (DEQ 2006b pp 8 33) The table also explains whether the score increases or decreases with environmental stress
As Table 13 indicates each of the eight VSCI metrics is calculated individually Each formula provides a unitless score based on a 100-point scale with 0 as the worst possible score and 100 as the best Any metric that provides a result above 100 is changed to 100 in order for the index to work properly After the eight metrics are calculated they are summed and averaged to provide a final VSCI score on a 100-point scale
Based on statistical analysis of Virginiarsquos streams and their BMI communities the DEQ (2006b p 8) currently uses the following tiers to interpret VSCI scores
VSCI Scores Aquatic Life Use
(ALI) Tiers
lt 42 severely stressed
42-55 moderately stressed
55-63 unknownfair (gray zone)
63-73 good
gt73 excellent
Section 2 of this report presents the results of calculations for the six BMI sampling sites the research team studied in the Hiatt-Lick Run subwatershed in 2008
o
p
1 Total taxa the number of distinct taxa in the sample measures the overall diversity of the macroinvertebrate assemblage The best standard value (BSV) is 189 Score = 100 x (X189) Total taxa decreases with stress 2 EPT taxa the sum of the distinct taxa in the orders Ephemeroptera (mayflies) Plecoptera (stoneflies) and Trichoptera (caddisflies) These orders are known to be sensitive to pollution The best standard value (BSV) is 117 Score = 100 x (X117) EPT taxa decreases with stress 3 Percent Ephemeroptera the percent abundance of individuals in the sample that belong to the order Ephemeroptera (mayflies) The best standard value (BSV) is 477 Score = 100 x (X477) Percent Ephemeroptera decreases with stress 4 Percent Plecoptera + Trichoptera less Hydropsychidae the percent abundance of individuals in the sample that belong to the orders Plecoptera (stoneflies) and Trichoptera (caddisflies) excluding the pollution-tolerant caddisfly family Hydropsychidae The best standard value (BSV) is 562 Score = 100 x (X562) Percent Plecoptera + Trichoptera less Hydropsychidae decreases with stress 5 Percent Scrapers the percent abundance of individuals in the sample whose primary functional mechanism for feeding is grazing on the substrate or periphyton (ie attached algae and associated material) The best standard value (BSV) is 467 Score = 100 x (X467) Percent scrapers decreases with stress 6 Percent Chironomidae the percent abundance of individuals in the sample that are Chironomidae (non-biting midge) larvae and pupae The best standard value (BSV) is 16 Score = 100 x [(100-X)(100-16)] Percent Chironomidae increases with stress 7 Percent Top Two Dominant the percent abundance of the two most frequent taxa present in the sample The best standard value (BSV) is 328 Score = 100 x [(100-X)(100-328)] The Percent Top 2 Dominant increases with stress 8 Hilsenhoff Biotic Index (HBI) an abundance-weighted average pollution tolerance of all the individuals within a sample keyed to the family level Tolerance ratings range from 1 (highly intolerant) to 10 (highly tolerant) The best standard value (BSV) is 29 Score = 100 x [(10-X)(10-29)] The Hilsenhoff Biotic Index (HBI) increases with stress ______________________________________________________________________________ Table 13 Description of Benthic Macroinvertebrate VSCI Metrics Includes responses (increases decreases) of each VSCI metric to degraded water quality conditions Adapted from Burton and Gerritsen (2003 pp 3-15 3-16 6-2) and DEQ (2008)
q
Appendix C
LABORATORY BENCH SHEETS
Shenandoah Universityrsquos Environmental Studies Department developed the Benthic Macroinvertebrate Laboratory Bench Sheet and VSCI Worksheet to record BMI specimen identifications and their VSCI classifications for each sample
This form is based in part on bench sheets developed by the EPA and Virginia DEQ for recording specimen identifications Additional columns on Shenandoah Universityrsquos forms for relative numbers and VSCI classifications make the calculation and verification of VSCI scores easier
Note the column for taxonomistrsquos initials Specimens were identified by students in Shenandoah Universityrsquos research group and then verified or where necessary changed by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality This external review helps assure the validity of the study Originals of these bench sheets with handwritten taxonomistsrsquo initials will be kept on file at Shenandoah Universityrsquos Environmental Studies Department for five years after the completion of this study
r
s
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-1 STATION NAME Mouth LAT N 39deg 12749rsquo (upstream) LONG W 078deg 04209rsquo (upstream) LAT N 39deg 12729rsquo (downstream) LONG W 078deg 04148rsquo (downstream) FORM COMPILED BY Jeremy Tovar W S Bousquet RIVER BASIN Potomac River INVESTIGATORS Marie Dahl Brandon Millholand J W Pangle Sean Robertson Jeremy Tovar Woodward Bousquet Ben Bousquet
Collection date 21 May 2008 Sheet completion date 12 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Hirudinea 3 33 JT BVW PR 8 SP Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Coenagrionidae 2 22 BVW PR 9 CR Ephemeroptera (mayflies) Caenidae 1 11 JT BVW CG 6 SP Ephemerllidae 4 44 JT BVW CG 2 CR Heptageniidae 5 55 JT BVW SC 3 CG Isonychiidae 1 11 BVW CF 3 CG Plecoptera (stoneflies) Perlidae 14 154 JT BVW PR 2 CR Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Corydalidae 1 11 JT BVW PR 5 CR Coleoptera (beetles) Elmidae 24 264 I JT BVW SC 4 CG Dytiscidae 4 44 JT BVW PR 6 GN Psephenidae 3 33 JT BVW SC 4 CG Diptera (true flies) Tipulidae 3 33 JT BVW SH 4 BU Chironomidae 26 286 JT BVW CG 6 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
t
Other Total Individuals 91 1001 Total Taxa 13
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
u
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-1 STATION NAME Mouth LAT N 39deg 12749rsquo (upstream) LONG W 078deg 04209rsquo (upstream) LAT N 39deg 12729rsquo (downstream) LONG W 078deg 04148rsquo (downstream) FORM COMPILED BY Jeremy Tovar W S Bousquet RIVER BASIN Potomac River INVESTIGATORS Brandon Millholand JW Pangle Jeremy Tovar Heather Kepple
Collection date 10 October 2008 Sheet completion date 12 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Oligochaeta 3 35 JT BVW CG 8 BU Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Coenagrionidae 2 23 JT BVW PR 9 CR Ephemeroptera (mayflies) Heptageniidae 12 140 JT BVW SC 3 CG Isonychiidae 2 23 BVW CF 3 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Hydropsychidae 16 186 JT BVW CF 6 CG Philopotamidae 31 360 JT BVW CF 2 CG Hemiptera (true bugs) (Veliidae) (5) - JT BVW ( ) Megaloptera (dobsonflies etc) Corydalidae 10 116 JT BVW PR 5 CR Coleoptera (beetles) Dytiscidae 1 12 JT BVW PR 6 GN Psephenidae 7 81 JT BVW SC 4 CG Diptera (true flies) Tipulidae 2 23 BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
v
Other Total Individuals 86
(91) 999
Total Taxa 10 (11)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009 Veliidae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
w
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-2 STATION NAME Package Plant LAT N 39deg 12958rsquo (upstream) LONG W 078deg 04900 (upstream) LAT N 39deg 12958rsquo (downstream) LONG W 078deg 04822 (downstream) FORM COMPILED BY JW Pangle W Bousquet RIVER BASIN Potomac River INVESTIGATORS JW Pangle S Robertson J Tovar M Dahl B Millholland W Bousquet
Collection date 29 May 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Plecoptera (stoneflies) Perlidae 11 136 I JP BVW PR 1 CR Trichoptera (caddisflies) Hydropsychidae 10 123 I JP BVW CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 14 173 I JP BVW SC 4 CG Diptera (true flies) Chironomidae 32 395 I JP BVW CG 6 BU Simuliidae 14 173 I JP BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
x
Other Total Individuals 81 1000 Total Taxa 5
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-2 STATION NAME Package Plant LAT N 39deg 12958rsquo (upstream) LONG W 078deg 04900 (upstream) LAT N 39deg 12958rsquo (downstream) LONG W 078deg 04822 (downstream) FORM COMPILED BY JW Pangle W Bousquet RIVER BASIN Potomac River INVESTIGATORS JW Pangle Brandon Millholland Jeremy Tovar Heather Kepple
Collection date 10 October 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Heptageniidae 40 392 I WBJP SC 3 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Hydropsychidae 7 69 I JP WB BVW CF 6 CG Philopotamidae 28 275 I JP WB BVW CF 2 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Corydalidae 6 59 I JP WB BVW PR 5 CR Coleoptera (beetles) Psephenidae 7 69 I JP WB BVW SC 4 CG Elmidae 8 78 I JP WB BVW SC 4 CG Diptera (true flies) Tipulidae 6 59 I JP WB BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
y
z
Other Total Individuals 102 1001 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-3 STATION NAME Rt 664 Bridge LAT N 39deg 12934rsquo (upstream) LONG W 078deg 05019rsquo (upstream) LAT N 39deg 12951rsquo (downstream) LONG W 078deg 04958rsquo (downstream) FORM COMPILED BY S Robertson W Bousquet RIVER BASIN Potomac River INVESTIGATORS SRobertson BMillholland WBousquet MDahl JPangle
Collection date 28 May 2008 Sheet completion date 11 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Leptophlebiidae 1 15 I SGR BVW CG 2 CR Baetidae 1 15 I BVW CG 5 CG Plecoptera (stoneflies) Perlidae 3 46 I SGR BVW PR 1 CR Nemouridae 1 15 I BVW SH 3 CR Trichoptera (caddisflies) Hydropsychidae 5 77 I SGR BVW CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 27 415 I SGR BVW SC 4 CG Psephenidae 2 31 A SGR BVW SC 4 CG Diptera (true flies) Simuliidae 5 77 I SGR BVW CF 6 CG Chironomidae 20 308 I SGR BVW CG 6 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
aa
bb
Other Total Individuals 65 999 Total Taxa 9
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-3 STATION NAME Rt 664 Bridge LAT N 39deg 12934rsquo (upstream) LONG W 078deg 05019rsquo (upstream) LAT N 39deg 12951rsquo (downstream) LONG W 078deg 04958rsquo (downstream) FORM COMPILED BY S Robertson RIVER BASIN Potomac River INVESTIGATORS S Robertson M Dahl A Fisher H Kepple
Collection date 10 Oct 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Heptageniidae 2 20 I SGR SC 3 CG Isonychiidae 2 20 I BVW CF 2 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Philopotamidae 36 367 I SGR CF 2 CG Hydropsychidae 47 480 I SGR CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 2 20 A SGR SC 4 CG Psephenidae 8 82 A SGR SC 4 CG Diptera (true flies) Tabanidae 1 10 I SGR PR 7 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
cc
dd
Other Total Individuals 98 999 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-4 STATION NAME Unnamed Tributary LAT (upstream) N 39deg 13035rsquo LONG (upstream) W 078deg 05129rsquo LAT (downstream) N 39deg 13000rsquo LONG (downstream) W 078deg 5086rsquo FORM COMPILED BY A Fisher M Dahl rev W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson A Fisher H Kepple
Collection date 31 May 2008 Sheet completion date rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Flatworms (Turbellaria) Worms (Oligochaeta) Naididae 1 14 - BVW CG 8 BU Leeches (Hirudinea) Snails (Gastropoda) Clams (Bivalvia) Isopods (Isopoda) Asellidae 3 42 A AF BVW CG 8 SP Scuds (Amphipoda) Crayfish Shrimp (Decapoda) Water Mites (Hydracarina) Dragonflies (Odonata Anisoptera) Damselflies (Odonata Zygoptera) Mayflies (Ephemeroptera) Stoneflies (Plecoptera) Perlidae 2 28 I AF BVW PR 1 CR Caddisflies (Trichoptera) True Bugs (Hemiptera) Corixidae 1 14 I AF BVW MP 9 DV Dobsonflies (Megaloptera) Beetles (Coleoptera) Dytiscidae 1 14 I AF BVW PR 6 GN True Flies (Diptera) Chironomidae 61 847 I AF BVW CG 6 BU Simuliidae 3 42 I AF BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
ee
ff
Other Total Individuals 72 1001 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-4 STATION NAME Unnamed Tributary LAT (upstream) N 39deg 13035rsquo LONG (upstream) W 078deg 05129rsquo LAT (downstream) N 39deg 13000rsquo LONG (downstream) W 078deg 5086rsquo FORM COMPILED BY Andrew G M Fisher W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson A Fisher H Kepple
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Flatworms (Turbellaria) Worms (Oligochaeta) Lumbriculidae 2 32 A AF BVW CG 8 BU Leeches (Hirudinea) Snails (Gastropoda) Lymnaeidae 1 16 A BVW CG 9 GN Clams (Bivalvia) Isopods (Isopoda) Scuds (Amphipoda) Crayfish Shrimp (Decapoda) Cambaridae 1 16 A AF BVW GN 5 GN Water Mites (Hydracarina) Dragonflies (Odonata Anisoptera) Damselflies (Odonata Zygoptera) Calopterygidae 4 63 I BVW PR 7 CL Mayflies (Ephemeroptera) Tricorythidae 1 16 I BVW CG 5 SP Stoneflies (Plecoptera) Caddisflies (Trichoptera) Phryganeidae 4 63 I AF BVW SH 4 CR Hydropsychidae 6 95 I AF BVW CF 6 CG I True Bugs (Hemiptera) Corixidae 8 127 I AF BVW MP 9 DV (Veliidae ) (7) - I AF BVW ( ) Dobsonflies (Megaloptera) Sialidae 3 48 I AF BVW PR 7 SP Corydalidae 2 32 I AF BVW PR 5 CR Beetles (Coleoptera) Haliplidae 1 16 A AF BVW MP 8 GN Dysticidae 7 111 I AF BVW PR 6 GN Hydrophilidae 1 16 A AF BVW PR 8 GN Elmidae 3 48 I BVW SC 4 CG True Flies (Diptera) Chironomidae 8 127 I AF BVW CG 6 BU Simuliidae 7 111 I AF BVW CF 6 CG Tipulidae 4 63 I AF BVW SH 4 GU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
gg
hh
Other Total Individuals 63
(70) 1000
Total Taxa 17 (18)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Veliidae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-5 STATION NAME Lick Run LAT N 39deg 12752rsquo (upstream) LONG W 078deg 05685rsquo (upstream) LAT N 39deg 12784rsquo (downstream) LONG W 078deg 05631rsquo (downstream) FORM COMPILED BY Brandon Millholland W Bousquet RIVER BASIN Potomac River INVESTIGATORS J Pangle S Robertson W Bousquet JTovar M Dahl
Collection date 29 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Gomphidae 1 15 I BVW PR 3 BU Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Leptophlebiidae 11 167 A LBM BVW CG 2 CR Plecoptera (stoneflies) Perlidae 10 152 A LBM BVW PR 1 CR Perlodiae 1 15 A LBM BVW PR 2 CR Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 5 76 A LBM BVW SC 4 CG Elmidae 2 30 I LBM BVW SC 4 CG Diptera (true flies) (Culicidae) (3) - P LMB BVW ( ) Chironomidae 23 348 I LBM BVW CG 6 BU Simuliidae 13 197 I LBM BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
ii
jj
Other Total Individuals 66
(69) 1000
Total Taxa 7 (8)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Culicidae (Diptera) pupae not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl J Pangle S Robertson W Bousquet B Millholland J Tovar H Kepple
Collection date 30 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 1 12 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Asellidae 114 965 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Plecoptera (stoneflies) Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 2 23 A MRD BVW SC 4 CG Diptera (true flies)
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
kk
ll
Other Total Individuals 117 1000 Total Taxa 3
Notes Subsample taken 29 October 2009 All taxonomic determinations by MRD were confirmed by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson K McCann A Fisher H Kepple A Barrett
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 2 17 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Sphaeriidae 2 17 I MRD BVW CF 8 BU Isopoda (isopods) Asellidae 99 853 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Calopterygidae 2 17 A MRD BVW PR 7 CL Ephemeroptera (mayflies) Leptophlebiidae 2 17 I MRD BVW CG 2 CR Heptageniidae 4 34 A MRD BVW SC 3 CG Plecoptera (stoneflies) Perlidae 1 09 A MRD BVW PR 1 CR Trichoptera (caddisflies) Hemiptera (true bugs) (Gerridae) (1) - A MRD BVW ( ) Corixidae 1 09 A MRD BVW PR 5 CR Megaloptera (dobsonflies etc) Coleoptera (beetles) Dryopidae 1 09 A MRD BVW SC 5 CG Diptera (true flies) Tipulidae 2 17 I BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
mm
nn
Other Total Individuals 116
(117) 999
Total Taxa 10 (11)
Notes Subsample taken 6 November 2008 Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009 Gerridae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
- Disclaimer ii
- Title Page iii
- Acknowledgements v
- Table of Contents vii
- SECTION 2 RESULTS DATA TABLES 7
- OVERVIEW AND FINDINGS
- Introduction
- RESULTS DATA TABLES
- REFERENCES
- FIELD DATA SHEETS
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- SubstrateType
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- WATER QUALITY ANALYSIS
- THROUGH THE VIRGINIA
- STREAM CONDITION INDEX (VSCI)
- LABORATORY BENCH SHEETS
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
viii
Section 1
OVERVIEW AND FINDINGS
Introduction
This report presents baseline water quality data for Hiatt Run and Lick Run -- termed the Hiatt-Lick Run subwatershed in this study Located in Frederick County Virginia Hiatt and Lick Runs are tributaries of Opequon Creek The Opequon in turn empties into the Potomac River and then the Chesapeake Bay The investigation was conducted by a Shenandoah University research team in 2008 Researchers used benthic macroinvertebrates (BMIs) as biological indicators of water quality This report presents an overview of the study followed by summary tables and a list of references The Appendix contains data sheets and a description of the data analysis These data will form part of the baseline water quality assessment that is being undertaken through the Opequon Creek Targeted Watershed Grant The grant administered by Jim Lawrence reflects a broad-based partnership that includes Virginia Tech Shenandoah University the Frederick-Winchester Service Authority The Opequon Watershed (TOW) citizens organization as well as federal state and local governments community groups and business interests Grant partners will use best management practices (BMPs) to reduce nutrient runoff and address other pollution problems in the Opequon Creek watershed Since 1996 Opequon Creek has appeared on the impaired waters list prepared by the Virginia Department of Environmental Quality (DEQ) The Hiatt-Lick Run subwatershed was selected for this study because it represents changes that are typical of the Opequon Creek watershed These changes include new commercial and residential developments as well as the implementation of water quality improvement practices such as tree planting in riparian zones fences to prevent livestock from entering streams and replacing outdated sewage treatment facilities such as a package plant on lower Hiatt Run
Seven students from Shenandoah Universityrsquos Environmental Studies Department conducted this study from May through December 2008 under the direction of Woodward Bousquet Professor of Environmental Studies and Biology The research team followed procedures described in the Rapid Bioassessment Protocols (RBPs) of the US Environmental Protection Agency (Barbour et al 1999) and the Virginia Stream Condition Index (VSCI) The VSCI provides an evaluation of water quality based on a streamrsquos benthic macroinvertebrate community (Burton and Gerritsen 2003 DEQ 2006b and 2008)
In consultation with Billy van Wart of the DEQ and grant administrator Jim Lawrence the Shenandoah University researchers selected six representative sampling sites in the Hiatt-Lick Run subwatershed Each site was given a name and an HLR (for Hiatt-Lick Run) code Figure 1 maps their positions while their characteristics and coordinates appear in the field data sheets (see Appendix A) These sites were established according to EPA and DEQ guidelines that generally call for a 100-meter-long stream reach with at least three riffle zones a width sufficient to accommodate a 1-meter seine and a substrate composed of least 35 cobbles
Specific locations of the sampling sites on Hiatt and Lick Runs were selected to reflect different watershed positions and changing land uses See Figure 1 Further information about each sampling site appears in the field data sheets (Appendix A)
1
Figure 1 Sampling Sites in the Hiatt-Lick Run Subwatershed of Opequon Creek Frederick County Virginia Scale 1 centimeter = 500 meters
HLR-1 (Mouth) measures water quality at the lowest point of the subwatershed as Hiatt Run flows into Opequon Creek Forests cover the immediate riparian zones on both sides of Hiatt Run This site will be used in future years by the DEQ for BMI monitoring HLR-2 (Package Plant) is situated on Hiatt Run just below the outflow pipe of an outdated package plant that formerly treated sewage from the Jordan Springs area During the study period the package plant was taken off-line and a pumping station was constructed The Frederick-Winchester Service Authority now treats this sewage at its Opequon Water Reclamation Facility HLR-3 (Route 664 Bridge) was chosen because it is located upstream from the package plant but downstream of the confluence of Hiatt Run and Lick Run HLR-4 (Unnamed Tributary) is located downstream of the Snowden Bridge residential development which has been under construction The area immediately upstream of this sampling site was being graded by heavy equipment during the May 2008 sampling time HLR-5 (Lick Run) the researchers learned dries up in late summer It had a modest but constant flow during when the May 2008 sample was taken HLR-6 (McCann Farm) represents the headwater areas of the Hiatt-Lick Run subwatershed of Opequon Creek The McCanns who graze cattle in their pastures that surround Hiatt Run here have been working with the Potomac Conservancy to implement Best Management Practices
2
(BMPs) to improve water quality These practices include fencing cattle out of the stream and spring runs and planting more trees in the riparian zone
Methods and Analysis
The research team established a sampling plan and then sampled according to methods the DEQ developed for the VSCI Using a 1 meter x 1 meter seine samples from 2m2 of substrate in riffle zones were to be collected twice at each site once in May 2008 and once in October 2008 At five of the six sites stream characteristics permitted the standard single-habitat sampling procedure At the McCann Farm (Site HLR-6) researchers needed to use multi-habitat sampling See Barbour et al (1999 pp 7-3 ff) for further descriptions of these two methods
All six sites were sampled in May 2008 Low-water conditions in October 2008 prevented the Shenandoah University group from collecting BMIs at Lick Run (HLR-5) that month but the other five sites were sampled successfully As a result the research team obtained eleven samples for analysis in this study Samples were preserved in ethanol for subsequent identification
DEQ procedures require subsampling when the number of BMIs in a given sample exceeds 100 All eleven samples in this study met that criterion Consequently researchers used a Caton tray to produce random subsamples of 90 to 110 specimens from each sample During the analysis that followed some specimens in the subsamples were rejected either because they turned out to be terrestrial species or because they were in the wrong life stage (eg pupae) In most cases the researchers identified specimens to the family level Primary taxonomic references were A Guide to Common Freshwater Invertebrates of North America (Voshell 2002) Fresh-Water Invertebrates of the United States (Pennak 1953) and An Introduction to the Aquatic Insects of North America (Merritt and Cummins 1996) DEQ Regional Aquatic Biologist Billy van Wart reviewed verified and where necessary corrected the identifications
The investigators then used the Virginia Stream Condition Index (VSCI) to produce water quality scores based on the BMIs collected A VSCI score is based on eight different measurement formulas called metrics Each metric is a numeric measure of a biological communityrsquos species composition diversity ecological niches or tolerance to pollution When combined into a single multimetric VSCI score the response of the benthic community to water conditions can be evaluated For further information and worksheets see Appendices B and C respectively
Findings
Table 1 presents the VSCI scores for the eleven samples collected in the Hiatt-Lick Run subwatershed in 2008 Since a sample year consists of two samples the mean scores for each site for the entire year are also included As the table shows
bull None of the 11 samples was rated excellent bull Only 1 of the 11 samples was rated as good bull Only 2 of the 11 samples were rated fair (ie in the ldquogrey zonerdquo between impaired and
unimpaired) bull The remaining 8 samples were in either moderately stressed or severely stressed
condition
3
Site Name and Code Sampling
Date of
TaxaVCSI Score
Water Quality Rating
Mouth (HLR-1) 21 May 2008 13 571 Fair (Gray Zone)
Mouth (HLR-1) 10 Oct 2008 10 608 Fair (Gray Zone)
site average for 2008 590 Fair (Gray Zone)
Package Plant (HLR-2) 29 May 2008 5 370 Severely Stressed
Package Plant (HLR-2) 10 Oct 2008 7 674 Good
site average for 2008 522 Moderately Stressed
Route 664 Bridge (HLR-3) 28 May 2008 9 485 Moderately Stressed
Route 664 Bridge (HLR-3) 10 Oct 2008 7 469 Moderately Stressed
site average for 2008 477 Moderately Stressed
Unnamed Tributary (HLR-4) 31 May 2008 7 173 Severely Stressed
Unnamed Tributary (HLR-4) 10 Oct 2008 17 476 Moderately Stressed
site average for 2008 325 Severely Stressed
Upper Lick Run (HLR-5) 29 May 2008 7 455 Moderately Stressed
site average for 2008 455 Moderately Stressed
McCann Farm (HLR-6) 30 May 2008 3 190 Severely Stressed
McCann Farm (HLR-6) 10 Oct 2008 10 316 Severely Stressed
site average for 2008 253 Severely Stressed
Summary 6 sites 11 samples
36 BMI taxa
VSCI = Virginia Stream Condition Index see Burton and Gerritsen (2003) and Virginia Department of Environmental Quality (2006b and 2008) Table 1 Summary Table of Virginia Stream Condition Index (VSCI) Scores and Water Quality Ratings for Sites in the Hiatt-Lick Run Subwatershed of Opequon Creek Frederick County Virginia 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
4
Improvements in water quality ratings at three sites between May and October suggest that BMPs and other changes may already be having some positive effects on water quality At the Package Plant (Site HLR-2) the VSCI score rose dramatically from 370 (severely stressed) to 674 (good) The small-scale sewage treatment plant as noted above was taken off-line during this period Similarly water quality at the Unnamed Tributary (HLR-4) increased from 173 (severely stressed) to 476 (moderately stressed) Adjacent construction was completed over the summer and grass was planted on the property Management practices on the McCann Farm (HLR-6) may be responsible for improving conditions for aquatic life in Hiatt Runrsquos headwaters Even though the water quality was still rated as severely stressed in October the VSCI score rose from 190 to 316 at this sampling site Further studies are needed to determine whether the changes noted in 2008 persist into the future In 2003 the DEQ began a Total Maximum Daily Load (TMDL) process for Opequon Creek as required by the Water Quality Act of 1987 The TMDL approach establishes a ldquopollution budgetrdquo for each impaired stream for each type of impairment and for each responsible pollutant In conjunction with the DEQ and the Virginia Department of Conservation and Recreation (DCR) Virginia Polytechnic Institutersquos Department of Biosystems Engineering developed a TMDL implementation plan (Opequon Creek IP Steering Committee et al 2006) Monitoring the BMI communities of Opequon Creek and its tributaries is essential to evaluating the progress of the proposed water quality improvement projects
Future Research The relatively small Hiatt-Lick Run subwatershed provides several case studies that can help assess the effectiveness of various water quality management practices not only in Opequon Creek but also in other watersheds in the Chesapeake Bay region and beyond In supporting that goal this study makes two principal contributions 1 It provides baseline data to measure the improvement or degradation of the Hiatt-Lick Run section of the Opequon Creek watershed and 2 It establishes a monitoring framework for the long-term biological monitoring of water quality and the evaluation of best management practices (BMPs) This framework consists of
bull Sampling Sites ndash six stream reaches in the Hiatt-Lick Run subwatershed selected as sampling sites according to EPA and DEQ specifications
bull Data Collection Protocols ndash based on the EPArsquos Rapid Bioassessment Protocols (RBPs)
(Barbour et al 1999) and the DEQrsquos Virginia Stream Index (VSCI) (Burton and Gerritsen 2003 DEQ 2006b and 2008) and
bull Analytical Procedures ndash contained in the Virginia Stream Condition Index (VSCI see
Burton and Gerritsen 2003 DEQ 2006b pp 8 and 33 and DEQ 2008 pp 29-34)
5
6
Section 2
RESULTS DATA TABLES
Site HLR-1 Mouth
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 13 189 6872 EPT taxa 5 117 4273 Ephemeroptera 121 477 2544 Plec +Tric less Hydropsych 154 562 2745 Scrapers 352 467 7536 Chironomidae 286 16 7267 Top 2 Dominant 550 328 6708 Hilsenhoff Biotic Index (HBI) 45 29 775VSCI Score 571
According to the DEQrsquos water quality standards aquatic life at this site is fair (gray zone) See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 2 VSCI Scores for Mouth (HLR-1) Site in Hiatt-Lick Run Sampled on 21 May 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 10 189 5292 EPT taxa 4 117 3423 Ephemeroptera 163 477 3424 Plec +Tric less Hydropsych 360 562 6415 Scrapers 221 467 4736 Chironomidae 00 16 10007 Top 2 Dominant 546 328 6768 Hilsenhoff Biotic Index (HBI) 39 29 859VSCI Score 608
According to the DEQrsquos water quality standards aquatic life at this site is fair (gray zone) See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 3 VSCI Scores for Mouth (HLR-1) Site in Hiatt-Lick Run Sampled 10 October 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
7
Site HLR-2 Package Plant
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 5 189 2642 EPT taxa 2 117 1713 Ephemeroptera 00 477 004 Plec +Tric less Hydropsych 110 562 1965 Scrapers 173 467 3706 Chironomidae 395 16 6157 Top 2 Dominant 568 328 6438 Hilsenhoff Biotic Index (HBI) 50 29 704VSCI Score 370
According to the DEQrsquos water quality standards aquatic life at this site is severely stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 4 VSCI Scores for Package Plant (HLR-2) Site in Hiatt-Lick Run Sampled 29 May 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 7 189 3702 EPT taxa 34 117 2563 Ephemeroptera 392 477 8214 Plec +Tric less Hydropsych 275 562 4895 Scrapers 539 467 10006 Chironomidae 00 16 10007 Top 2 Dominant 667 328 4968 Hilsenhoff Biotic Index (HBI) 32 29 958VSCI Score 674
According to the DEQrsquos water quality standards aquatic life at this site is good See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 5 VSCI Scores for Package Plant (HLR-2) Site in Hiatt-Lick Run Sampled 10 October 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
8
Site HLR-3 Route 664 Bridge
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 9 189 4762 EPT taxa 5 117 4273 Ephemeroptera 30 477 634 Plec +Tric less Hydropsych 61 562 1095 Scrapers 446 467 9556 Chironomidae 308 16 7037 Top 2 Dominant 723 328 4128 Hilsenhoff Biotic Index (HBI) 48 29 732VSCI Score 485
According to the DEQrsquos water quality standards aquatic life at this site is moderately stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 6 VSCI Scores for Route 664 Bridge (HLR-3) Site in Hiatt-Lick Run Sampled 28 May 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 7 189 3702 EPT taxa 4 117 3423 Ephemeroptera 40 477 844 Plec +Tric less Hydropsych 367 562 6535 Scrapers 122 467 2616 Chironomidae 00 16 10007 Top 2 Dominant 847 328 2288 Hilsenhoff Biotic Index (HBI) 42 29 817VSCI Score 469
According to the DEQrsquos water quality standards aquatic life at this site is moderately stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 7 VSCI Scores for Route 664 Bridge (HLR-3) Site in Hiatt-Lick Run Sampled 10 October 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
9
Site HLR-4 Unnamed Tributary
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 7 189 3702 EPT taxa 1 117 853 Ephemeroptera 00 477 004 Plec +Tric less Hydropsych 28 562 495 Scrapers 00 467 006 Chironomidae 847 16 1557 Top 2 Dominant 889 328 1658 Hilsenhoff Biotic Index (HBI) 60 29 563VSCI Score 173
According to the DEQrsquos water quality standards aquatic life at this site is severely stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 8 VSCI Scores for Unnamed Tributary (HLR-4) Site in Hiatt-Lick Run Sampled 31 May 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 17 189 8992 EPT taxa 3 117 2563 Ephemeroptera 16 477 344 Plec +Tric less Hydropsych 63 562 1125 Scrapers 48 467 1036 Chironomidae 127 16 8877 Top 2 Dominant 254 328 10008 Hilsenhoff Biotic Index (HBI) 63 29 521VSCI Score 476
According to the DEQrsquos water quality standards aquatic life at this site is moderately stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 9 VSCI Scores for Unnamed Tributary (HLR-4) Site in Hiatt-Lick Run Sampled 10 October 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
10
Site HLR-5 Lick Run
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 7 189 3702 EPT taxa 3 117 2563 Ephemeroptera 167 477 3504 Plec +Tric less Hydropsych 167 562 2975 Scrapers 106 467 2276 Chironomidae 348 16 6637 Top 2 Dominant 545 328 6778 Hilsenhoff Biotic Index (HBI) 43 29 803VSCI Score 455
According to the DEQrsquos water quality standards aquatic life at this site is moderately stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 10 VSCI Scores for Lick Run (HLR-5) Site in Hiatt-Lick Run Sampled 29 May 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
Note
Water conditions at the Lick Run (HLR-5) site were too low in October 2008 to permit a sample to be taken
11
Site HLR-6 McCann Farm
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 3 189 1592 EPT taxa 0 117 003 Ephemeroptera 00 477 004 Plec +Tric less Hydropsych 00 562 005 Scrapers 23 467 496 Chironomidae 00 16 10007 Top 2 Dominant 988 328 188 Hilsenhoff Biotic Index (HBI) 79 29 296VSCI Score 190
According to the DEQrsquos water quality standards aquatic life at this site is severely stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 11 VSCI Scores for McCann Farm (HLR-6) Site in Hiatt-Lick Run Sampled 30 May 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 10 189 5292 EPT taxa 3 117 2563 Ephemeroptera 51 477 1074 Plec +Tric less Hydropsych 09 562 165 Scrapers 43 467 926 Chironomidae 00 16 10007 Top 2 Dominant 887 328 1688 Hilsenhoff Biotic Index (HBI) 75 29 352VSCI Score 315
According to the DEQrsquos water quality standards aquatic life at this site is severely stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 12 VSCI Scores for McCann Farm (HLR-6) Site in Hiatt-Lick Run Sampled 10 October 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
12
REFERENCES
Barbour Michael T Jeroen Gerritsen Blaine D Snyder and James B Stribling 1999 Rapid bioassessment protocols for use in streams and wadeable rivers periphyton benthic macroinvertebrates and fish Second edition EPA 841-B-99-002 Washington DC US Environmental Protection Agency Office of Water Available online at httpwwwepagovOWOWmonitoringbioasspshtml Burton June and Jeroen Gerritsen 2003 A stream condition index for Virginia non-coastal streams Prepared for the US EPA Office of Science and Technology US EPA Region 3 Environmental Services Division and Virginia Department of Environmental Quality Owings Mills Maryland Tetra Tech Inc Available online at httpwwwdeqvirginiagovwatermonitoringpdf vastrmconpdf Hessler Rachel A Andrea R Martin Heidi D Ruggeri William B Seymour Jr and Woodward S Bousquet 2006 Water quality assessment using benthic macroinvertebrates A study and compilation Winchester Virginia Environmental Studies Program Shenandoah University Occasional publication no 10 Igoe Robert 2006 DEQ identifies nine impaired streams in Clarke Frederick The Winchester Star 15 July B1 Jackson Charlie 2005 Survey targets Opequon cleanup The Winchester Star 19 August B1 Johnston Elizabeth A and Woodward S Bousquet 2003 What happens when it rains A study of water quality in Abrams Creek and Town Run Winchester and Frederick County Virginia Winchester Virginia Environmental Studies Program Shenandoah University Occasional publication no7 Merritt Richard W and Kenneth W Cummins 1996 An introduction to the aquatic insects of North America Dubuque Iowa KendallHunt Publishing Company Opequon Creek IP Steering Committee Virginia Tech Department of Biological Systems Engineering and Center for TMDL and Watershed Studies University of Virginia Institute for Environmental Negotiation and West Virginia University Division of Resource Management 2006 Opequon Creek watershed TMDL implementation plan Blacksburg Virginia Virginia Tech Department of Biological Systems Engineering Available online at httpwwwtmdlbsevteduoutreachC82 Pennak Robert W 1953 Fresh-water invertebrates of the United States New York New York The Ronald Press Company Rosenburg David M and Vincent H Resh 1993 Freshwater biomonitoring and benthic macroinvertebrates New York New York Chapman amp Hall Shenandoah University Public Relations Office 2002 Data collected by SU and its partners helps identify impaired water Winchester Virginia Available online at httpwwwsuedutemp_ newscfmurlnum=32 Thorp James H and Alan P Covich 1991 Ecology and classification of North American freshwater invertebrates San Diego California Academic Press Inc
13
US Environmental Protection Agency 1997 Volunteer stream monitoring a methods manual EPA 841-B-97-003 Washington DC US Environmental Protection Agency Office of Water Van Meter Val 2003 Opequon Creek pollution limits to be established The Winchester Star 4 April B1 Virginia Department of Environmental Quality 2006a Virginia 305(b)303(d) water quality integrated report to Congress and the EPA Administrator for the period January 1 2000 to December 31 2004 Richmond Virginia Virginia Department of Environmental Quality Available online at httpwwwdeqvirginiagovwqair2006html __________ 2006b Using probabilistic data to validate the non-coastal Virginia Stream Condition Index (draft) VDEQ technical bulletin WQA2006-001 Virginia Department of Environmental Quality Available online at httpwwwloudounwatershedorgstream_assessmentreference_documents VA_DEQ_SCI_Validationpdf __________ 2008 Biological monitoring program quality assurance project plan for wadeable streams and rivers Richmond Virginia Virginia Department of Environmental Quality Update available online at httpwwwdeqstatevausbiomonitoringdocumentsBioMonQAPP_13Aug2008pdf Virginia Tech Aquatic Entomology Laboratory 2005 Taxa dictionary [Excel file] Blacksburg Virginia Virginia Tech Department of Biological Systems Engineering 2003a Opequon watershed TMDLs for benthic impairments Abrams Creek and Lower Opequon Creek Frederick and Clarke Counties Virginia Prepared for the Virginia Department of Environmental Quality and Virginia Department of Conservation and Recreation Blacksburg Virginia Available online at httpwwwdeqstate vaustmdlapptmdlsshenrvrabropebcpdf Voshell J Reese Jr 2002 A guide to common freshwater invertebrates of North America Blacksburg Virginia The McDonald amp Woodward Publishing Company
14
APPENDICES
15
16
a
Appendix A
FIELD DATA SHEETS
This appendix presents a Site Characterization Field Data Sheet for each of the six sampling reaches established on Hiatt Run and Lick Run for this study They were filled out during the sampling runs made in May 2008
These forms were adapted by Shenandoah University from forms used by the EPA and Virginia DEQ for recording characteristics of sampling sites
b
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Mouth STATION _HLR-1_____ STREAM CLASS LAT N 39 12749 (upstream) LONG W 078 04209(upstream) LAT N 39 12729 (downstream) LONG W 078 04148 (downstream) FORM COMPILED BY Jeremy Tovar RIVER BASIN Potomac River INVESTIGATORS JPangle B Millholland MDahl SRobertson W Bousquet JTovar BBousquet
DATE May 21 2008_________ TIME_250 PM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature __24___degC showers (intermittent) Comments _______________________
___ cloud cover _0_ ______________________________
X clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x ( Significant springs andor seeps) x (Valley ) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS
c
d
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence x FieldPasture Industrial Obvious sources Agricultural Other ____________ Stream scour steep slope erosion x Residential Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left Field and young thin deciduous forest 100 Bank=Grass cobble and shrub Right 90 maturing deciduous forest 45 shale slope Bank=Bedrock soil and herbs Indicate the dominant vegetation type(s) and record the dominant species
x Trees Shrubs x (Grasses) Forbs Dominant species Oak species and mix deciduous
INSTREAM FEATURES
Measured reach length ___100__m Canopy Cover Estimated stream width _35__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area __350_m2
Estimated stream depth _008_m Proportion of Reach Represented by Stream High water mark _15_m Morphology Types Surface velocity (at thalweg) _05__msec x Riffle__75___ x Run__20___ x Pool__5___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___00__ m2 Density of LWD _-_ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __15_____ x Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present Algae
WATER QUALITY Temperature __19___degC Water Odors x NormalNone Sewage Specific Conductance - Petroleum Chemical Fishy Other___________________ Dissolved Oxygen - Water Surface Oils Slick Sheen Globs Flecks pH __-_ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used _____NA________ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic x None Relict shells Other______None_____ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 8 Detritus sticks wood coarse plant materials
0 Boulder gt256 mm (10rsquorsquo) 0
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 51 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 40 Sand 006-2 mm (gritty) 1 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University
SITE CHARACTERIZATION FIELD DATA SHEET FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Package Plant STATION HLR-2 STREAM CLASS LAT N 39 129580 (upstream) LONG W 078 04900 (upstream) LAT N 39 129581 (downstream) LONG W 078 04822 (downstream) FORM COMPILED BY JW Pangle RIVER BASIN Potomac River INVESTIGATORS JPangle JTovar MDahl B Millholland SRobertson W Bousquet
DATE__19 May 2008_ TIME__1240__ PM
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature _____degC showers (intermittent) Comments __Sunny Day_____________
_5__ cloud cover _0__ ______________________________
x clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x (Significant springs andor seeps) x (Valley) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS
e
f
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence FieldPasture Industrial Obvious sources Agricultural Other ____________ __Upstream Construction and Stream bank Scour Residential __________________________________ Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left Deciduous forest with thick herb and shrub layers bank= vegetation and rocks Right Deciduous forest with thick herb and shrub layers bank=vegetation soil and rocks Indicate the dominant vegetation type(s) and record the dominant species
x Trees x Shrubs Grasses Forbs Dominant species Black Walnut White Ash Hackberry and American Sycamore
INSTREAM FEATURES
Measured reach length 100 m Canopy Cover Estimated stream width _35__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area __350_m2
Estimated stream depth __15__m Proportion of Reach Represented by Stream High water mark __22___m Morphology Types Surface velocity (at thalweg) _035__msec x Riffle__30___ x Run__30___ x Pool__40___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___10____ m2 Density of LWD _______ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __30___ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present__________________________________________________________
WATER QUALITY Temperature __18___degC Water Odors x NormalNone Sewage Specific Conductance __-____ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ____-____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used _____NA____ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other___None_____ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach SubstrateType Characteristic
Composition in Sampling Reach
Bedrock 3 Detritus sticks wood coarse plant materials
2 Boulder gt256 mm (10rsquorsquo) 5
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 70 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 22 Sand 006-2 mm (gritty) 0 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiattrsquos Run LOCATION Rt 664 Bridge STATION HLR-3 STREAM CLASS LAT N 39 12934 (upstream) LONG W 078 05019 (upstream) LAT N 39 12958 (downstream) LONG W 078 04958 (downstream) FORM COMPILED BY Sean Robertson RIVER BASIN Potomac River INVESTIGATORS SRobertson BMillholland WBousquet MDahl JPangle
DATE 28 May 2008 TIME 1127 AM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality 2008
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature __14___degC showers (intermittent) x Trace Comments _______________________
_65 x cloud cover ___ ______________________________
x clearsunny __________________________________
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x (Significant springs andor seeps) x (Valley) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS
g
h
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence Some evidence x FieldPasture Industrial x Obvious sources Agricultural x Other Old Package Plant Downstream x Residential Bridge and Road Power line and Pump station Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left 70 field 30 shrubs and deciduous trees Bank=Soil and vegetation Right Maturing deciduous forest steep slope Bank=Shale Bedrock Indicate the dominant vegetation type(s) and record the dominant species
x Trees x Shrubs x Grasses Forbs Dominant species Sycamore and Red Oaks
INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width __51___m Fully open x Partly open Partly shaded Fully shaded Sampling reach area _510_m2
Estimated stream depth __135___m Proportion of Reach Represented by Stream High water mark __-___m Morphology Types Surface velocity (at thalweg) __20___msec x Riffle_40_ x Run_50_ x Pool 10 Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___0____ m2 Density of LWD ___-____ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation ___50____ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present Brown Algae
WATER QUALITY Temperature __17___degC Water Odors x NormalNone Sewage Specific Conductance ___-___ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ___-_____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used ____NA_______ Clear x Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other____None____ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 15 Detritus sticks wood coarse plant materials
0 Boulder gt256 mm (10rsquorsquo) 15
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 62 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 8 Sand 006-2 mm (gritty) 0 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Unnamed Tributary STATION HLR-4 STREAM CLASS LAT N 39 13035 (upstream) LONG W 078 05129 (upstream) LAT N 39 13000 (downstream) LONG W 078 05086 (downstream) FORM COMPILED BY Marie Dahl RIVER BASIN Potomac River INVESTIGATORS M Dahl HKepple SRobertson AFisher
DATE 31 May 2008 TIME 5 PM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality 2008
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) YES x NO
rain (steady rain) Air Temperature __22___degC showers (intermittent) Comments _______________________
_0__ cloud cover _0__ ______________________________
x clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area __-___ km2 mi2 Montane Significant springs andor seeps x Valley Marsh or swamp Montane amp Valley Mixture of origins Other ___________________
COMMENTS Snowden Bridge development (upstream) and sewer line
i
j
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence x FieldPasture Industrial Obvious sources Agricultural Other ____________ Construction upstream and adjacent x Residential Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left Forested with rocky banks field across silt fence and 2m tree strip Right Forested with some rocky banks and forest through the highway Indicate the dominant vegetation type(s) and record the dominant species
x Trees x Shrubs x Grasses Forbs Dominant species White Ash and Hackberry
INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width ___38__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area __380__m2
Estimated stream depth __12__cm Proportion of Reach Represented by Stream High water mark __2___m Morphology Types Surface velocity (at thalweg) __46___msec x Riffle__25___ x Run__70___ x Pool__5___ Channelized Yes x No Dam Present x Yes No
LARGE WOODY DEBRIS
LWD _______ m2 Density of LWD _______ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation ___NA____ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present___Algae___
WATER QUALITY Temperature __16___degC Water Odors x NormalNone Sewage Specific Conductance __-____ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ___-_____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used ____NA_____ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells x Other___Shale_______ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 0 Detritus sticks wood coarse plant materials
10 Boulder gt256 mm (10rsquorsquo) 5
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 45 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 30 Sand 006-2 mm (gritty) 0 Marl grey shell
fragments 0
Silt 0004-006 mm 20 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Lick Run STATION HLR-5 STREAM CLASS LAT N 39 127517 (upstream) LONG W 078 05685(upstream) LAT N 39 12784 (downstream) LONG W 078 05631 (downstream) FORM COMPILED BY B Millholland RIVER BASIN Potomac River INVESTIGATORS B Millholland JTovar SRobertson MDahl WBousquet JPangle
DATE 29 May 2008 TIME 1040 AM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project
k
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature __15___degC showers (intermittent) Comments _______________________
_5_ X cloud cover _0__ ______________________________
x clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane Significant springs andor seeps x Valley Marsh or swamp Montane amp Valley Mixture of origins Other ___________________
COMMENTS Lick Run appears to be intermittent and may be dry in Fall 2008
l
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence x FieldPasture Industrial Obvious sources Agricultural Other ____________ Adjacent logging stream bank scour Residential Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left 85 young deciduous forest 15 logged Bank=Soil vegetation and cobbles Right Young deciduous forest on 30 slope Bank= Soil vegetation and cobble Indicate the dominant vegetation type(s) and record the dominant species
x Trees Shrubs Grasses Forbs Dominant species Mixed Deciduous INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width __11__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area 110 m2
Estimated stream depth _009__m Proportion of Reach Represented by Stream High water mark __96___m Morphology Types Surface velocity (at thalweg) __01___msec x Riffle__30___ x Run__5___ x Pool__65___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___0____ m2 Density of LWD ___-____ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __70___ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present__________________________________________________________
WATER QUALITY Temperature __14___degC Water Odors x NormalNone Sewage Specific Conductance __-__ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ___-___ Water Surface Oils Slick Sheen Globs Flecks pH __-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used _____NA______ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other_____None_____
SEDIMENT AND SUBSTRATE
Odors Deposits Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other________________ x Other Slightly Salty Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 0 Detritus sticks wood coarse plant materials
5 Boulder gt256 mm (10rsquorsquo) 0
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 75 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 23 Sand 006-2 mm (gritty) 2 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION McCann Farm STATION HLR-6 STREAM CLASS LAT N 39 13076 (upstream) LONG W 078 06539 (upstream) LAT N 39 13063 (downstream) LONG W 078 06473 (downstream) FORM COMPILED BY MDahl RIVER BASIN Potomac River INVESTIGATORS MDahl JPangle SRobertson W Bousqet BMillholland JTovar H Kepple
DATE 30 May 2008 TIME 1050 AM (start)
PROJECT NAME
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) YES x NO
rain (steady rain) Air Temperature __18___degC showers (intermittent) Comments _______________________
_85_x cloud cover _10__ ______________________________
clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x (Significant springs andor seeps) x ( Valley) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS Multi-habitat sampling with D-frame nets and seine
m
n
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial x No evidence Some evidence x FieldPasture Industrial Obvious sources x Agricultural Other ____________ Minor stream bank scour Residential Local Watershed Erosion x None Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left 35 bank grasses scattered trees and some bushes Right High bank with grasses and scattered trees Indicate the dominant vegetation type(s) and record the dominant species
x Trees Shrubs Grasses Forbs Dominant species Black Walnut and Festuca elatior
INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width __2___m Fully open Partly open x Partly shaded Fully shaded Sampling reach area __200_m2
Estimated stream depth __03_m Proportion of Reach Represented by Stream High water mark __1___m Morphology Types Surface velocity (at thalweg) __166_msec x Riffle__0___ x Run__55___ x Pool__45___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___2____ m2 Density of LWD ___-____ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __20_____ x Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present ____Glyceria (grass)sp__________
WATER QUALITY Temperature __16___degC Water Odors x NormalNone Sewage Specific Conductance ___-___ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ____-____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used ______NA___ Clear x Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other__NONE__ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 0 Detritus sticks wood coarse plant materials
5 Boulder gt256 mm (10rsquorsquo) 0
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 27 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 38 Sand 006-2 mm (gritty) 5 Marl grey shell
fragments 0 (lt1)
Silt 0004-006 mm 25 Clay lt 0004 mm (slick) 5 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Appendix B
WATER QUALITY ANALYSIS THROUGH THE VIRGINIA
STREAM CONDITION INDEX (VSCI)
The Virginia Stream Condition Index (VSCI) relies on eight metrics to categorize stream health According to Burton and Gerritsen (2003 p 1-1) this index ldquohellip can be used as a primary indicator of ecosystem health and can identify impairment with respect to the reference (or natural) conditionrdquo These metrics are numeric measures of a biological communityrsquos species composition diversity ecological niches or tolerance to pollution
Table 13 on the next page describes each VSCI metric lists its formula and provides the best standard value (BSV) for that metric Based on a 100-point scale the BSV is the score that reflects optimum conditions for aquatic life ie the best water quality value possible (DEQ 2006b pp 8 33) The table also explains whether the score increases or decreases with environmental stress
As Table 13 indicates each of the eight VSCI metrics is calculated individually Each formula provides a unitless score based on a 100-point scale with 0 as the worst possible score and 100 as the best Any metric that provides a result above 100 is changed to 100 in order for the index to work properly After the eight metrics are calculated they are summed and averaged to provide a final VSCI score on a 100-point scale
Based on statistical analysis of Virginiarsquos streams and their BMI communities the DEQ (2006b p 8) currently uses the following tiers to interpret VSCI scores
VSCI Scores Aquatic Life Use
(ALI) Tiers
lt 42 severely stressed
42-55 moderately stressed
55-63 unknownfair (gray zone)
63-73 good
gt73 excellent
Section 2 of this report presents the results of calculations for the six BMI sampling sites the research team studied in the Hiatt-Lick Run subwatershed in 2008
o
p
1 Total taxa the number of distinct taxa in the sample measures the overall diversity of the macroinvertebrate assemblage The best standard value (BSV) is 189 Score = 100 x (X189) Total taxa decreases with stress 2 EPT taxa the sum of the distinct taxa in the orders Ephemeroptera (mayflies) Plecoptera (stoneflies) and Trichoptera (caddisflies) These orders are known to be sensitive to pollution The best standard value (BSV) is 117 Score = 100 x (X117) EPT taxa decreases with stress 3 Percent Ephemeroptera the percent abundance of individuals in the sample that belong to the order Ephemeroptera (mayflies) The best standard value (BSV) is 477 Score = 100 x (X477) Percent Ephemeroptera decreases with stress 4 Percent Plecoptera + Trichoptera less Hydropsychidae the percent abundance of individuals in the sample that belong to the orders Plecoptera (stoneflies) and Trichoptera (caddisflies) excluding the pollution-tolerant caddisfly family Hydropsychidae The best standard value (BSV) is 562 Score = 100 x (X562) Percent Plecoptera + Trichoptera less Hydropsychidae decreases with stress 5 Percent Scrapers the percent abundance of individuals in the sample whose primary functional mechanism for feeding is grazing on the substrate or periphyton (ie attached algae and associated material) The best standard value (BSV) is 467 Score = 100 x (X467) Percent scrapers decreases with stress 6 Percent Chironomidae the percent abundance of individuals in the sample that are Chironomidae (non-biting midge) larvae and pupae The best standard value (BSV) is 16 Score = 100 x [(100-X)(100-16)] Percent Chironomidae increases with stress 7 Percent Top Two Dominant the percent abundance of the two most frequent taxa present in the sample The best standard value (BSV) is 328 Score = 100 x [(100-X)(100-328)] The Percent Top 2 Dominant increases with stress 8 Hilsenhoff Biotic Index (HBI) an abundance-weighted average pollution tolerance of all the individuals within a sample keyed to the family level Tolerance ratings range from 1 (highly intolerant) to 10 (highly tolerant) The best standard value (BSV) is 29 Score = 100 x [(10-X)(10-29)] The Hilsenhoff Biotic Index (HBI) increases with stress ______________________________________________________________________________ Table 13 Description of Benthic Macroinvertebrate VSCI Metrics Includes responses (increases decreases) of each VSCI metric to degraded water quality conditions Adapted from Burton and Gerritsen (2003 pp 3-15 3-16 6-2) and DEQ (2008)
q
Appendix C
LABORATORY BENCH SHEETS
Shenandoah Universityrsquos Environmental Studies Department developed the Benthic Macroinvertebrate Laboratory Bench Sheet and VSCI Worksheet to record BMI specimen identifications and their VSCI classifications for each sample
This form is based in part on bench sheets developed by the EPA and Virginia DEQ for recording specimen identifications Additional columns on Shenandoah Universityrsquos forms for relative numbers and VSCI classifications make the calculation and verification of VSCI scores easier
Note the column for taxonomistrsquos initials Specimens were identified by students in Shenandoah Universityrsquos research group and then verified or where necessary changed by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality This external review helps assure the validity of the study Originals of these bench sheets with handwritten taxonomistsrsquo initials will be kept on file at Shenandoah Universityrsquos Environmental Studies Department for five years after the completion of this study
r
s
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-1 STATION NAME Mouth LAT N 39deg 12749rsquo (upstream) LONG W 078deg 04209rsquo (upstream) LAT N 39deg 12729rsquo (downstream) LONG W 078deg 04148rsquo (downstream) FORM COMPILED BY Jeremy Tovar W S Bousquet RIVER BASIN Potomac River INVESTIGATORS Marie Dahl Brandon Millholand J W Pangle Sean Robertson Jeremy Tovar Woodward Bousquet Ben Bousquet
Collection date 21 May 2008 Sheet completion date 12 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Hirudinea 3 33 JT BVW PR 8 SP Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Coenagrionidae 2 22 BVW PR 9 CR Ephemeroptera (mayflies) Caenidae 1 11 JT BVW CG 6 SP Ephemerllidae 4 44 JT BVW CG 2 CR Heptageniidae 5 55 JT BVW SC 3 CG Isonychiidae 1 11 BVW CF 3 CG Plecoptera (stoneflies) Perlidae 14 154 JT BVW PR 2 CR Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Corydalidae 1 11 JT BVW PR 5 CR Coleoptera (beetles) Elmidae 24 264 I JT BVW SC 4 CG Dytiscidae 4 44 JT BVW PR 6 GN Psephenidae 3 33 JT BVW SC 4 CG Diptera (true flies) Tipulidae 3 33 JT BVW SH 4 BU Chironomidae 26 286 JT BVW CG 6 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
t
Other Total Individuals 91 1001 Total Taxa 13
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
u
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-1 STATION NAME Mouth LAT N 39deg 12749rsquo (upstream) LONG W 078deg 04209rsquo (upstream) LAT N 39deg 12729rsquo (downstream) LONG W 078deg 04148rsquo (downstream) FORM COMPILED BY Jeremy Tovar W S Bousquet RIVER BASIN Potomac River INVESTIGATORS Brandon Millholand JW Pangle Jeremy Tovar Heather Kepple
Collection date 10 October 2008 Sheet completion date 12 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Oligochaeta 3 35 JT BVW CG 8 BU Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Coenagrionidae 2 23 JT BVW PR 9 CR Ephemeroptera (mayflies) Heptageniidae 12 140 JT BVW SC 3 CG Isonychiidae 2 23 BVW CF 3 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Hydropsychidae 16 186 JT BVW CF 6 CG Philopotamidae 31 360 JT BVW CF 2 CG Hemiptera (true bugs) (Veliidae) (5) - JT BVW ( ) Megaloptera (dobsonflies etc) Corydalidae 10 116 JT BVW PR 5 CR Coleoptera (beetles) Dytiscidae 1 12 JT BVW PR 6 GN Psephenidae 7 81 JT BVW SC 4 CG Diptera (true flies) Tipulidae 2 23 BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
v
Other Total Individuals 86
(91) 999
Total Taxa 10 (11)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009 Veliidae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
w
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-2 STATION NAME Package Plant LAT N 39deg 12958rsquo (upstream) LONG W 078deg 04900 (upstream) LAT N 39deg 12958rsquo (downstream) LONG W 078deg 04822 (downstream) FORM COMPILED BY JW Pangle W Bousquet RIVER BASIN Potomac River INVESTIGATORS JW Pangle S Robertson J Tovar M Dahl B Millholland W Bousquet
Collection date 29 May 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Plecoptera (stoneflies) Perlidae 11 136 I JP BVW PR 1 CR Trichoptera (caddisflies) Hydropsychidae 10 123 I JP BVW CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 14 173 I JP BVW SC 4 CG Diptera (true flies) Chironomidae 32 395 I JP BVW CG 6 BU Simuliidae 14 173 I JP BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
x
Other Total Individuals 81 1000 Total Taxa 5
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-2 STATION NAME Package Plant LAT N 39deg 12958rsquo (upstream) LONG W 078deg 04900 (upstream) LAT N 39deg 12958rsquo (downstream) LONG W 078deg 04822 (downstream) FORM COMPILED BY JW Pangle W Bousquet RIVER BASIN Potomac River INVESTIGATORS JW Pangle Brandon Millholland Jeremy Tovar Heather Kepple
Collection date 10 October 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Heptageniidae 40 392 I WBJP SC 3 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Hydropsychidae 7 69 I JP WB BVW CF 6 CG Philopotamidae 28 275 I JP WB BVW CF 2 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Corydalidae 6 59 I JP WB BVW PR 5 CR Coleoptera (beetles) Psephenidae 7 69 I JP WB BVW SC 4 CG Elmidae 8 78 I JP WB BVW SC 4 CG Diptera (true flies) Tipulidae 6 59 I JP WB BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
y
z
Other Total Individuals 102 1001 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-3 STATION NAME Rt 664 Bridge LAT N 39deg 12934rsquo (upstream) LONG W 078deg 05019rsquo (upstream) LAT N 39deg 12951rsquo (downstream) LONG W 078deg 04958rsquo (downstream) FORM COMPILED BY S Robertson W Bousquet RIVER BASIN Potomac River INVESTIGATORS SRobertson BMillholland WBousquet MDahl JPangle
Collection date 28 May 2008 Sheet completion date 11 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Leptophlebiidae 1 15 I SGR BVW CG 2 CR Baetidae 1 15 I BVW CG 5 CG Plecoptera (stoneflies) Perlidae 3 46 I SGR BVW PR 1 CR Nemouridae 1 15 I BVW SH 3 CR Trichoptera (caddisflies) Hydropsychidae 5 77 I SGR BVW CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 27 415 I SGR BVW SC 4 CG Psephenidae 2 31 A SGR BVW SC 4 CG Diptera (true flies) Simuliidae 5 77 I SGR BVW CF 6 CG Chironomidae 20 308 I SGR BVW CG 6 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
aa
bb
Other Total Individuals 65 999 Total Taxa 9
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-3 STATION NAME Rt 664 Bridge LAT N 39deg 12934rsquo (upstream) LONG W 078deg 05019rsquo (upstream) LAT N 39deg 12951rsquo (downstream) LONG W 078deg 04958rsquo (downstream) FORM COMPILED BY S Robertson RIVER BASIN Potomac River INVESTIGATORS S Robertson M Dahl A Fisher H Kepple
Collection date 10 Oct 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Heptageniidae 2 20 I SGR SC 3 CG Isonychiidae 2 20 I BVW CF 2 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Philopotamidae 36 367 I SGR CF 2 CG Hydropsychidae 47 480 I SGR CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 2 20 A SGR SC 4 CG Psephenidae 8 82 A SGR SC 4 CG Diptera (true flies) Tabanidae 1 10 I SGR PR 7 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
cc
dd
Other Total Individuals 98 999 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-4 STATION NAME Unnamed Tributary LAT (upstream) N 39deg 13035rsquo LONG (upstream) W 078deg 05129rsquo LAT (downstream) N 39deg 13000rsquo LONG (downstream) W 078deg 5086rsquo FORM COMPILED BY A Fisher M Dahl rev W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson A Fisher H Kepple
Collection date 31 May 2008 Sheet completion date rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Flatworms (Turbellaria) Worms (Oligochaeta) Naididae 1 14 - BVW CG 8 BU Leeches (Hirudinea) Snails (Gastropoda) Clams (Bivalvia) Isopods (Isopoda) Asellidae 3 42 A AF BVW CG 8 SP Scuds (Amphipoda) Crayfish Shrimp (Decapoda) Water Mites (Hydracarina) Dragonflies (Odonata Anisoptera) Damselflies (Odonata Zygoptera) Mayflies (Ephemeroptera) Stoneflies (Plecoptera) Perlidae 2 28 I AF BVW PR 1 CR Caddisflies (Trichoptera) True Bugs (Hemiptera) Corixidae 1 14 I AF BVW MP 9 DV Dobsonflies (Megaloptera) Beetles (Coleoptera) Dytiscidae 1 14 I AF BVW PR 6 GN True Flies (Diptera) Chironomidae 61 847 I AF BVW CG 6 BU Simuliidae 3 42 I AF BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
ee
ff
Other Total Individuals 72 1001 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-4 STATION NAME Unnamed Tributary LAT (upstream) N 39deg 13035rsquo LONG (upstream) W 078deg 05129rsquo LAT (downstream) N 39deg 13000rsquo LONG (downstream) W 078deg 5086rsquo FORM COMPILED BY Andrew G M Fisher W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson A Fisher H Kepple
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Flatworms (Turbellaria) Worms (Oligochaeta) Lumbriculidae 2 32 A AF BVW CG 8 BU Leeches (Hirudinea) Snails (Gastropoda) Lymnaeidae 1 16 A BVW CG 9 GN Clams (Bivalvia) Isopods (Isopoda) Scuds (Amphipoda) Crayfish Shrimp (Decapoda) Cambaridae 1 16 A AF BVW GN 5 GN Water Mites (Hydracarina) Dragonflies (Odonata Anisoptera) Damselflies (Odonata Zygoptera) Calopterygidae 4 63 I BVW PR 7 CL Mayflies (Ephemeroptera) Tricorythidae 1 16 I BVW CG 5 SP Stoneflies (Plecoptera) Caddisflies (Trichoptera) Phryganeidae 4 63 I AF BVW SH 4 CR Hydropsychidae 6 95 I AF BVW CF 6 CG I True Bugs (Hemiptera) Corixidae 8 127 I AF BVW MP 9 DV (Veliidae ) (7) - I AF BVW ( ) Dobsonflies (Megaloptera) Sialidae 3 48 I AF BVW PR 7 SP Corydalidae 2 32 I AF BVW PR 5 CR Beetles (Coleoptera) Haliplidae 1 16 A AF BVW MP 8 GN Dysticidae 7 111 I AF BVW PR 6 GN Hydrophilidae 1 16 A AF BVW PR 8 GN Elmidae 3 48 I BVW SC 4 CG True Flies (Diptera) Chironomidae 8 127 I AF BVW CG 6 BU Simuliidae 7 111 I AF BVW CF 6 CG Tipulidae 4 63 I AF BVW SH 4 GU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
gg
hh
Other Total Individuals 63
(70) 1000
Total Taxa 17 (18)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Veliidae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-5 STATION NAME Lick Run LAT N 39deg 12752rsquo (upstream) LONG W 078deg 05685rsquo (upstream) LAT N 39deg 12784rsquo (downstream) LONG W 078deg 05631rsquo (downstream) FORM COMPILED BY Brandon Millholland W Bousquet RIVER BASIN Potomac River INVESTIGATORS J Pangle S Robertson W Bousquet JTovar M Dahl
Collection date 29 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Gomphidae 1 15 I BVW PR 3 BU Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Leptophlebiidae 11 167 A LBM BVW CG 2 CR Plecoptera (stoneflies) Perlidae 10 152 A LBM BVW PR 1 CR Perlodiae 1 15 A LBM BVW PR 2 CR Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 5 76 A LBM BVW SC 4 CG Elmidae 2 30 I LBM BVW SC 4 CG Diptera (true flies) (Culicidae) (3) - P LMB BVW ( ) Chironomidae 23 348 I LBM BVW CG 6 BU Simuliidae 13 197 I LBM BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
ii
jj
Other Total Individuals 66
(69) 1000
Total Taxa 7 (8)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Culicidae (Diptera) pupae not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl J Pangle S Robertson W Bousquet B Millholland J Tovar H Kepple
Collection date 30 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 1 12 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Asellidae 114 965 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Plecoptera (stoneflies) Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 2 23 A MRD BVW SC 4 CG Diptera (true flies)
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
kk
ll
Other Total Individuals 117 1000 Total Taxa 3
Notes Subsample taken 29 October 2009 All taxonomic determinations by MRD were confirmed by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson K McCann A Fisher H Kepple A Barrett
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 2 17 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Sphaeriidae 2 17 I MRD BVW CF 8 BU Isopoda (isopods) Asellidae 99 853 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Calopterygidae 2 17 A MRD BVW PR 7 CL Ephemeroptera (mayflies) Leptophlebiidae 2 17 I MRD BVW CG 2 CR Heptageniidae 4 34 A MRD BVW SC 3 CG Plecoptera (stoneflies) Perlidae 1 09 A MRD BVW PR 1 CR Trichoptera (caddisflies) Hemiptera (true bugs) (Gerridae) (1) - A MRD BVW ( ) Corixidae 1 09 A MRD BVW PR 5 CR Megaloptera (dobsonflies etc) Coleoptera (beetles) Dryopidae 1 09 A MRD BVW SC 5 CG Diptera (true flies) Tipulidae 2 17 I BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
mm
nn
Other Total Individuals 116
(117) 999
Total Taxa 10 (11)
Notes Subsample taken 6 November 2008 Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009 Gerridae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
- Disclaimer ii
- Title Page iii
- Acknowledgements v
- Table of Contents vii
- SECTION 2 RESULTS DATA TABLES 7
- OVERVIEW AND FINDINGS
- Introduction
- RESULTS DATA TABLES
- REFERENCES
- FIELD DATA SHEETS
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- SubstrateType
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- WATER QUALITY ANALYSIS
- THROUGH THE VIRGINIA
- STREAM CONDITION INDEX (VSCI)
- LABORATORY BENCH SHEETS
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
Section 1
OVERVIEW AND FINDINGS
Introduction
This report presents baseline water quality data for Hiatt Run and Lick Run -- termed the Hiatt-Lick Run subwatershed in this study Located in Frederick County Virginia Hiatt and Lick Runs are tributaries of Opequon Creek The Opequon in turn empties into the Potomac River and then the Chesapeake Bay The investigation was conducted by a Shenandoah University research team in 2008 Researchers used benthic macroinvertebrates (BMIs) as biological indicators of water quality This report presents an overview of the study followed by summary tables and a list of references The Appendix contains data sheets and a description of the data analysis These data will form part of the baseline water quality assessment that is being undertaken through the Opequon Creek Targeted Watershed Grant The grant administered by Jim Lawrence reflects a broad-based partnership that includes Virginia Tech Shenandoah University the Frederick-Winchester Service Authority The Opequon Watershed (TOW) citizens organization as well as federal state and local governments community groups and business interests Grant partners will use best management practices (BMPs) to reduce nutrient runoff and address other pollution problems in the Opequon Creek watershed Since 1996 Opequon Creek has appeared on the impaired waters list prepared by the Virginia Department of Environmental Quality (DEQ) The Hiatt-Lick Run subwatershed was selected for this study because it represents changes that are typical of the Opequon Creek watershed These changes include new commercial and residential developments as well as the implementation of water quality improvement practices such as tree planting in riparian zones fences to prevent livestock from entering streams and replacing outdated sewage treatment facilities such as a package plant on lower Hiatt Run
Seven students from Shenandoah Universityrsquos Environmental Studies Department conducted this study from May through December 2008 under the direction of Woodward Bousquet Professor of Environmental Studies and Biology The research team followed procedures described in the Rapid Bioassessment Protocols (RBPs) of the US Environmental Protection Agency (Barbour et al 1999) and the Virginia Stream Condition Index (VSCI) The VSCI provides an evaluation of water quality based on a streamrsquos benthic macroinvertebrate community (Burton and Gerritsen 2003 DEQ 2006b and 2008)
In consultation with Billy van Wart of the DEQ and grant administrator Jim Lawrence the Shenandoah University researchers selected six representative sampling sites in the Hiatt-Lick Run subwatershed Each site was given a name and an HLR (for Hiatt-Lick Run) code Figure 1 maps their positions while their characteristics and coordinates appear in the field data sheets (see Appendix A) These sites were established according to EPA and DEQ guidelines that generally call for a 100-meter-long stream reach with at least three riffle zones a width sufficient to accommodate a 1-meter seine and a substrate composed of least 35 cobbles
Specific locations of the sampling sites on Hiatt and Lick Runs were selected to reflect different watershed positions and changing land uses See Figure 1 Further information about each sampling site appears in the field data sheets (Appendix A)
1
Figure 1 Sampling Sites in the Hiatt-Lick Run Subwatershed of Opequon Creek Frederick County Virginia Scale 1 centimeter = 500 meters
HLR-1 (Mouth) measures water quality at the lowest point of the subwatershed as Hiatt Run flows into Opequon Creek Forests cover the immediate riparian zones on both sides of Hiatt Run This site will be used in future years by the DEQ for BMI monitoring HLR-2 (Package Plant) is situated on Hiatt Run just below the outflow pipe of an outdated package plant that formerly treated sewage from the Jordan Springs area During the study period the package plant was taken off-line and a pumping station was constructed The Frederick-Winchester Service Authority now treats this sewage at its Opequon Water Reclamation Facility HLR-3 (Route 664 Bridge) was chosen because it is located upstream from the package plant but downstream of the confluence of Hiatt Run and Lick Run HLR-4 (Unnamed Tributary) is located downstream of the Snowden Bridge residential development which has been under construction The area immediately upstream of this sampling site was being graded by heavy equipment during the May 2008 sampling time HLR-5 (Lick Run) the researchers learned dries up in late summer It had a modest but constant flow during when the May 2008 sample was taken HLR-6 (McCann Farm) represents the headwater areas of the Hiatt-Lick Run subwatershed of Opequon Creek The McCanns who graze cattle in their pastures that surround Hiatt Run here have been working with the Potomac Conservancy to implement Best Management Practices
2
(BMPs) to improve water quality These practices include fencing cattle out of the stream and spring runs and planting more trees in the riparian zone
Methods and Analysis
The research team established a sampling plan and then sampled according to methods the DEQ developed for the VSCI Using a 1 meter x 1 meter seine samples from 2m2 of substrate in riffle zones were to be collected twice at each site once in May 2008 and once in October 2008 At five of the six sites stream characteristics permitted the standard single-habitat sampling procedure At the McCann Farm (Site HLR-6) researchers needed to use multi-habitat sampling See Barbour et al (1999 pp 7-3 ff) for further descriptions of these two methods
All six sites were sampled in May 2008 Low-water conditions in October 2008 prevented the Shenandoah University group from collecting BMIs at Lick Run (HLR-5) that month but the other five sites were sampled successfully As a result the research team obtained eleven samples for analysis in this study Samples were preserved in ethanol for subsequent identification
DEQ procedures require subsampling when the number of BMIs in a given sample exceeds 100 All eleven samples in this study met that criterion Consequently researchers used a Caton tray to produce random subsamples of 90 to 110 specimens from each sample During the analysis that followed some specimens in the subsamples were rejected either because they turned out to be terrestrial species or because they were in the wrong life stage (eg pupae) In most cases the researchers identified specimens to the family level Primary taxonomic references were A Guide to Common Freshwater Invertebrates of North America (Voshell 2002) Fresh-Water Invertebrates of the United States (Pennak 1953) and An Introduction to the Aquatic Insects of North America (Merritt and Cummins 1996) DEQ Regional Aquatic Biologist Billy van Wart reviewed verified and where necessary corrected the identifications
The investigators then used the Virginia Stream Condition Index (VSCI) to produce water quality scores based on the BMIs collected A VSCI score is based on eight different measurement formulas called metrics Each metric is a numeric measure of a biological communityrsquos species composition diversity ecological niches or tolerance to pollution When combined into a single multimetric VSCI score the response of the benthic community to water conditions can be evaluated For further information and worksheets see Appendices B and C respectively
Findings
Table 1 presents the VSCI scores for the eleven samples collected in the Hiatt-Lick Run subwatershed in 2008 Since a sample year consists of two samples the mean scores for each site for the entire year are also included As the table shows
bull None of the 11 samples was rated excellent bull Only 1 of the 11 samples was rated as good bull Only 2 of the 11 samples were rated fair (ie in the ldquogrey zonerdquo between impaired and
unimpaired) bull The remaining 8 samples were in either moderately stressed or severely stressed
condition
3
Site Name and Code Sampling
Date of
TaxaVCSI Score
Water Quality Rating
Mouth (HLR-1) 21 May 2008 13 571 Fair (Gray Zone)
Mouth (HLR-1) 10 Oct 2008 10 608 Fair (Gray Zone)
site average for 2008 590 Fair (Gray Zone)
Package Plant (HLR-2) 29 May 2008 5 370 Severely Stressed
Package Plant (HLR-2) 10 Oct 2008 7 674 Good
site average for 2008 522 Moderately Stressed
Route 664 Bridge (HLR-3) 28 May 2008 9 485 Moderately Stressed
Route 664 Bridge (HLR-3) 10 Oct 2008 7 469 Moderately Stressed
site average for 2008 477 Moderately Stressed
Unnamed Tributary (HLR-4) 31 May 2008 7 173 Severely Stressed
Unnamed Tributary (HLR-4) 10 Oct 2008 17 476 Moderately Stressed
site average for 2008 325 Severely Stressed
Upper Lick Run (HLR-5) 29 May 2008 7 455 Moderately Stressed
site average for 2008 455 Moderately Stressed
McCann Farm (HLR-6) 30 May 2008 3 190 Severely Stressed
McCann Farm (HLR-6) 10 Oct 2008 10 316 Severely Stressed
site average for 2008 253 Severely Stressed
Summary 6 sites 11 samples
36 BMI taxa
VSCI = Virginia Stream Condition Index see Burton and Gerritsen (2003) and Virginia Department of Environmental Quality (2006b and 2008) Table 1 Summary Table of Virginia Stream Condition Index (VSCI) Scores and Water Quality Ratings for Sites in the Hiatt-Lick Run Subwatershed of Opequon Creek Frederick County Virginia 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
4
Improvements in water quality ratings at three sites between May and October suggest that BMPs and other changes may already be having some positive effects on water quality At the Package Plant (Site HLR-2) the VSCI score rose dramatically from 370 (severely stressed) to 674 (good) The small-scale sewage treatment plant as noted above was taken off-line during this period Similarly water quality at the Unnamed Tributary (HLR-4) increased from 173 (severely stressed) to 476 (moderately stressed) Adjacent construction was completed over the summer and grass was planted on the property Management practices on the McCann Farm (HLR-6) may be responsible for improving conditions for aquatic life in Hiatt Runrsquos headwaters Even though the water quality was still rated as severely stressed in October the VSCI score rose from 190 to 316 at this sampling site Further studies are needed to determine whether the changes noted in 2008 persist into the future In 2003 the DEQ began a Total Maximum Daily Load (TMDL) process for Opequon Creek as required by the Water Quality Act of 1987 The TMDL approach establishes a ldquopollution budgetrdquo for each impaired stream for each type of impairment and for each responsible pollutant In conjunction with the DEQ and the Virginia Department of Conservation and Recreation (DCR) Virginia Polytechnic Institutersquos Department of Biosystems Engineering developed a TMDL implementation plan (Opequon Creek IP Steering Committee et al 2006) Monitoring the BMI communities of Opequon Creek and its tributaries is essential to evaluating the progress of the proposed water quality improvement projects
Future Research The relatively small Hiatt-Lick Run subwatershed provides several case studies that can help assess the effectiveness of various water quality management practices not only in Opequon Creek but also in other watersheds in the Chesapeake Bay region and beyond In supporting that goal this study makes two principal contributions 1 It provides baseline data to measure the improvement or degradation of the Hiatt-Lick Run section of the Opequon Creek watershed and 2 It establishes a monitoring framework for the long-term biological monitoring of water quality and the evaluation of best management practices (BMPs) This framework consists of
bull Sampling Sites ndash six stream reaches in the Hiatt-Lick Run subwatershed selected as sampling sites according to EPA and DEQ specifications
bull Data Collection Protocols ndash based on the EPArsquos Rapid Bioassessment Protocols (RBPs)
(Barbour et al 1999) and the DEQrsquos Virginia Stream Index (VSCI) (Burton and Gerritsen 2003 DEQ 2006b and 2008) and
bull Analytical Procedures ndash contained in the Virginia Stream Condition Index (VSCI see
Burton and Gerritsen 2003 DEQ 2006b pp 8 and 33 and DEQ 2008 pp 29-34)
5
6
Section 2
RESULTS DATA TABLES
Site HLR-1 Mouth
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 13 189 6872 EPT taxa 5 117 4273 Ephemeroptera 121 477 2544 Plec +Tric less Hydropsych 154 562 2745 Scrapers 352 467 7536 Chironomidae 286 16 7267 Top 2 Dominant 550 328 6708 Hilsenhoff Biotic Index (HBI) 45 29 775VSCI Score 571
According to the DEQrsquos water quality standards aquatic life at this site is fair (gray zone) See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 2 VSCI Scores for Mouth (HLR-1) Site in Hiatt-Lick Run Sampled on 21 May 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 10 189 5292 EPT taxa 4 117 3423 Ephemeroptera 163 477 3424 Plec +Tric less Hydropsych 360 562 6415 Scrapers 221 467 4736 Chironomidae 00 16 10007 Top 2 Dominant 546 328 6768 Hilsenhoff Biotic Index (HBI) 39 29 859VSCI Score 608
According to the DEQrsquos water quality standards aquatic life at this site is fair (gray zone) See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 3 VSCI Scores for Mouth (HLR-1) Site in Hiatt-Lick Run Sampled 10 October 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
7
Site HLR-2 Package Plant
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 5 189 2642 EPT taxa 2 117 1713 Ephemeroptera 00 477 004 Plec +Tric less Hydropsych 110 562 1965 Scrapers 173 467 3706 Chironomidae 395 16 6157 Top 2 Dominant 568 328 6438 Hilsenhoff Biotic Index (HBI) 50 29 704VSCI Score 370
According to the DEQrsquos water quality standards aquatic life at this site is severely stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 4 VSCI Scores for Package Plant (HLR-2) Site in Hiatt-Lick Run Sampled 29 May 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 7 189 3702 EPT taxa 34 117 2563 Ephemeroptera 392 477 8214 Plec +Tric less Hydropsych 275 562 4895 Scrapers 539 467 10006 Chironomidae 00 16 10007 Top 2 Dominant 667 328 4968 Hilsenhoff Biotic Index (HBI) 32 29 958VSCI Score 674
According to the DEQrsquos water quality standards aquatic life at this site is good See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 5 VSCI Scores for Package Plant (HLR-2) Site in Hiatt-Lick Run Sampled 10 October 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
8
Site HLR-3 Route 664 Bridge
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 9 189 4762 EPT taxa 5 117 4273 Ephemeroptera 30 477 634 Plec +Tric less Hydropsych 61 562 1095 Scrapers 446 467 9556 Chironomidae 308 16 7037 Top 2 Dominant 723 328 4128 Hilsenhoff Biotic Index (HBI) 48 29 732VSCI Score 485
According to the DEQrsquos water quality standards aquatic life at this site is moderately stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 6 VSCI Scores for Route 664 Bridge (HLR-3) Site in Hiatt-Lick Run Sampled 28 May 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 7 189 3702 EPT taxa 4 117 3423 Ephemeroptera 40 477 844 Plec +Tric less Hydropsych 367 562 6535 Scrapers 122 467 2616 Chironomidae 00 16 10007 Top 2 Dominant 847 328 2288 Hilsenhoff Biotic Index (HBI) 42 29 817VSCI Score 469
According to the DEQrsquos water quality standards aquatic life at this site is moderately stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 7 VSCI Scores for Route 664 Bridge (HLR-3) Site in Hiatt-Lick Run Sampled 10 October 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
9
Site HLR-4 Unnamed Tributary
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 7 189 3702 EPT taxa 1 117 853 Ephemeroptera 00 477 004 Plec +Tric less Hydropsych 28 562 495 Scrapers 00 467 006 Chironomidae 847 16 1557 Top 2 Dominant 889 328 1658 Hilsenhoff Biotic Index (HBI) 60 29 563VSCI Score 173
According to the DEQrsquos water quality standards aquatic life at this site is severely stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 8 VSCI Scores for Unnamed Tributary (HLR-4) Site in Hiatt-Lick Run Sampled 31 May 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 17 189 8992 EPT taxa 3 117 2563 Ephemeroptera 16 477 344 Plec +Tric less Hydropsych 63 562 1125 Scrapers 48 467 1036 Chironomidae 127 16 8877 Top 2 Dominant 254 328 10008 Hilsenhoff Biotic Index (HBI) 63 29 521VSCI Score 476
According to the DEQrsquos water quality standards aquatic life at this site is moderately stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 9 VSCI Scores for Unnamed Tributary (HLR-4) Site in Hiatt-Lick Run Sampled 10 October 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
10
Site HLR-5 Lick Run
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 7 189 3702 EPT taxa 3 117 2563 Ephemeroptera 167 477 3504 Plec +Tric less Hydropsych 167 562 2975 Scrapers 106 467 2276 Chironomidae 348 16 6637 Top 2 Dominant 545 328 6778 Hilsenhoff Biotic Index (HBI) 43 29 803VSCI Score 455
According to the DEQrsquos water quality standards aquatic life at this site is moderately stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 10 VSCI Scores for Lick Run (HLR-5) Site in Hiatt-Lick Run Sampled 29 May 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
Note
Water conditions at the Lick Run (HLR-5) site were too low in October 2008 to permit a sample to be taken
11
Site HLR-6 McCann Farm
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 3 189 1592 EPT taxa 0 117 003 Ephemeroptera 00 477 004 Plec +Tric less Hydropsych 00 562 005 Scrapers 23 467 496 Chironomidae 00 16 10007 Top 2 Dominant 988 328 188 Hilsenhoff Biotic Index (HBI) 79 29 296VSCI Score 190
According to the DEQrsquos water quality standards aquatic life at this site is severely stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 11 VSCI Scores for McCann Farm (HLR-6) Site in Hiatt-Lick Run Sampled 30 May 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 10 189 5292 EPT taxa 3 117 2563 Ephemeroptera 51 477 1074 Plec +Tric less Hydropsych 09 562 165 Scrapers 43 467 926 Chironomidae 00 16 10007 Top 2 Dominant 887 328 1688 Hilsenhoff Biotic Index (HBI) 75 29 352VSCI Score 315
According to the DEQrsquos water quality standards aquatic life at this site is severely stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 12 VSCI Scores for McCann Farm (HLR-6) Site in Hiatt-Lick Run Sampled 10 October 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
12
REFERENCES
Barbour Michael T Jeroen Gerritsen Blaine D Snyder and James B Stribling 1999 Rapid bioassessment protocols for use in streams and wadeable rivers periphyton benthic macroinvertebrates and fish Second edition EPA 841-B-99-002 Washington DC US Environmental Protection Agency Office of Water Available online at httpwwwepagovOWOWmonitoringbioasspshtml Burton June and Jeroen Gerritsen 2003 A stream condition index for Virginia non-coastal streams Prepared for the US EPA Office of Science and Technology US EPA Region 3 Environmental Services Division and Virginia Department of Environmental Quality Owings Mills Maryland Tetra Tech Inc Available online at httpwwwdeqvirginiagovwatermonitoringpdf vastrmconpdf Hessler Rachel A Andrea R Martin Heidi D Ruggeri William B Seymour Jr and Woodward S Bousquet 2006 Water quality assessment using benthic macroinvertebrates A study and compilation Winchester Virginia Environmental Studies Program Shenandoah University Occasional publication no 10 Igoe Robert 2006 DEQ identifies nine impaired streams in Clarke Frederick The Winchester Star 15 July B1 Jackson Charlie 2005 Survey targets Opequon cleanup The Winchester Star 19 August B1 Johnston Elizabeth A and Woodward S Bousquet 2003 What happens when it rains A study of water quality in Abrams Creek and Town Run Winchester and Frederick County Virginia Winchester Virginia Environmental Studies Program Shenandoah University Occasional publication no7 Merritt Richard W and Kenneth W Cummins 1996 An introduction to the aquatic insects of North America Dubuque Iowa KendallHunt Publishing Company Opequon Creek IP Steering Committee Virginia Tech Department of Biological Systems Engineering and Center for TMDL and Watershed Studies University of Virginia Institute for Environmental Negotiation and West Virginia University Division of Resource Management 2006 Opequon Creek watershed TMDL implementation plan Blacksburg Virginia Virginia Tech Department of Biological Systems Engineering Available online at httpwwwtmdlbsevteduoutreachC82 Pennak Robert W 1953 Fresh-water invertebrates of the United States New York New York The Ronald Press Company Rosenburg David M and Vincent H Resh 1993 Freshwater biomonitoring and benthic macroinvertebrates New York New York Chapman amp Hall Shenandoah University Public Relations Office 2002 Data collected by SU and its partners helps identify impaired water Winchester Virginia Available online at httpwwwsuedutemp_ newscfmurlnum=32 Thorp James H and Alan P Covich 1991 Ecology and classification of North American freshwater invertebrates San Diego California Academic Press Inc
13
US Environmental Protection Agency 1997 Volunteer stream monitoring a methods manual EPA 841-B-97-003 Washington DC US Environmental Protection Agency Office of Water Van Meter Val 2003 Opequon Creek pollution limits to be established The Winchester Star 4 April B1 Virginia Department of Environmental Quality 2006a Virginia 305(b)303(d) water quality integrated report to Congress and the EPA Administrator for the period January 1 2000 to December 31 2004 Richmond Virginia Virginia Department of Environmental Quality Available online at httpwwwdeqvirginiagovwqair2006html __________ 2006b Using probabilistic data to validate the non-coastal Virginia Stream Condition Index (draft) VDEQ technical bulletin WQA2006-001 Virginia Department of Environmental Quality Available online at httpwwwloudounwatershedorgstream_assessmentreference_documents VA_DEQ_SCI_Validationpdf __________ 2008 Biological monitoring program quality assurance project plan for wadeable streams and rivers Richmond Virginia Virginia Department of Environmental Quality Update available online at httpwwwdeqstatevausbiomonitoringdocumentsBioMonQAPP_13Aug2008pdf Virginia Tech Aquatic Entomology Laboratory 2005 Taxa dictionary [Excel file] Blacksburg Virginia Virginia Tech Department of Biological Systems Engineering 2003a Opequon watershed TMDLs for benthic impairments Abrams Creek and Lower Opequon Creek Frederick and Clarke Counties Virginia Prepared for the Virginia Department of Environmental Quality and Virginia Department of Conservation and Recreation Blacksburg Virginia Available online at httpwwwdeqstate vaustmdlapptmdlsshenrvrabropebcpdf Voshell J Reese Jr 2002 A guide to common freshwater invertebrates of North America Blacksburg Virginia The McDonald amp Woodward Publishing Company
14
APPENDICES
15
16
a
Appendix A
FIELD DATA SHEETS
This appendix presents a Site Characterization Field Data Sheet for each of the six sampling reaches established on Hiatt Run and Lick Run for this study They were filled out during the sampling runs made in May 2008
These forms were adapted by Shenandoah University from forms used by the EPA and Virginia DEQ for recording characteristics of sampling sites
b
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Mouth STATION _HLR-1_____ STREAM CLASS LAT N 39 12749 (upstream) LONG W 078 04209(upstream) LAT N 39 12729 (downstream) LONG W 078 04148 (downstream) FORM COMPILED BY Jeremy Tovar RIVER BASIN Potomac River INVESTIGATORS JPangle B Millholland MDahl SRobertson W Bousquet JTovar BBousquet
DATE May 21 2008_________ TIME_250 PM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature __24___degC showers (intermittent) Comments _______________________
___ cloud cover _0_ ______________________________
X clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x ( Significant springs andor seeps) x (Valley ) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS
c
d
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence x FieldPasture Industrial Obvious sources Agricultural Other ____________ Stream scour steep slope erosion x Residential Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left Field and young thin deciduous forest 100 Bank=Grass cobble and shrub Right 90 maturing deciduous forest 45 shale slope Bank=Bedrock soil and herbs Indicate the dominant vegetation type(s) and record the dominant species
x Trees Shrubs x (Grasses) Forbs Dominant species Oak species and mix deciduous
INSTREAM FEATURES
Measured reach length ___100__m Canopy Cover Estimated stream width _35__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area __350_m2
Estimated stream depth _008_m Proportion of Reach Represented by Stream High water mark _15_m Morphology Types Surface velocity (at thalweg) _05__msec x Riffle__75___ x Run__20___ x Pool__5___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___00__ m2 Density of LWD _-_ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __15_____ x Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present Algae
WATER QUALITY Temperature __19___degC Water Odors x NormalNone Sewage Specific Conductance - Petroleum Chemical Fishy Other___________________ Dissolved Oxygen - Water Surface Oils Slick Sheen Globs Flecks pH __-_ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used _____NA________ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic x None Relict shells Other______None_____ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 8 Detritus sticks wood coarse plant materials
0 Boulder gt256 mm (10rsquorsquo) 0
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 51 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 40 Sand 006-2 mm (gritty) 1 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University
SITE CHARACTERIZATION FIELD DATA SHEET FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Package Plant STATION HLR-2 STREAM CLASS LAT N 39 129580 (upstream) LONG W 078 04900 (upstream) LAT N 39 129581 (downstream) LONG W 078 04822 (downstream) FORM COMPILED BY JW Pangle RIVER BASIN Potomac River INVESTIGATORS JPangle JTovar MDahl B Millholland SRobertson W Bousquet
DATE__19 May 2008_ TIME__1240__ PM
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature _____degC showers (intermittent) Comments __Sunny Day_____________
_5__ cloud cover _0__ ______________________________
x clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x (Significant springs andor seeps) x (Valley) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS
e
f
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence FieldPasture Industrial Obvious sources Agricultural Other ____________ __Upstream Construction and Stream bank Scour Residential __________________________________ Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left Deciduous forest with thick herb and shrub layers bank= vegetation and rocks Right Deciduous forest with thick herb and shrub layers bank=vegetation soil and rocks Indicate the dominant vegetation type(s) and record the dominant species
x Trees x Shrubs Grasses Forbs Dominant species Black Walnut White Ash Hackberry and American Sycamore
INSTREAM FEATURES
Measured reach length 100 m Canopy Cover Estimated stream width _35__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area __350_m2
Estimated stream depth __15__m Proportion of Reach Represented by Stream High water mark __22___m Morphology Types Surface velocity (at thalweg) _035__msec x Riffle__30___ x Run__30___ x Pool__40___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___10____ m2 Density of LWD _______ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __30___ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present__________________________________________________________
WATER QUALITY Temperature __18___degC Water Odors x NormalNone Sewage Specific Conductance __-____ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ____-____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used _____NA____ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other___None_____ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach SubstrateType Characteristic
Composition in Sampling Reach
Bedrock 3 Detritus sticks wood coarse plant materials
2 Boulder gt256 mm (10rsquorsquo) 5
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 70 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 22 Sand 006-2 mm (gritty) 0 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiattrsquos Run LOCATION Rt 664 Bridge STATION HLR-3 STREAM CLASS LAT N 39 12934 (upstream) LONG W 078 05019 (upstream) LAT N 39 12958 (downstream) LONG W 078 04958 (downstream) FORM COMPILED BY Sean Robertson RIVER BASIN Potomac River INVESTIGATORS SRobertson BMillholland WBousquet MDahl JPangle
DATE 28 May 2008 TIME 1127 AM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality 2008
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature __14___degC showers (intermittent) x Trace Comments _______________________
_65 x cloud cover ___ ______________________________
x clearsunny __________________________________
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x (Significant springs andor seeps) x (Valley) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS
g
h
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence Some evidence x FieldPasture Industrial x Obvious sources Agricultural x Other Old Package Plant Downstream x Residential Bridge and Road Power line and Pump station Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left 70 field 30 shrubs and deciduous trees Bank=Soil and vegetation Right Maturing deciduous forest steep slope Bank=Shale Bedrock Indicate the dominant vegetation type(s) and record the dominant species
x Trees x Shrubs x Grasses Forbs Dominant species Sycamore and Red Oaks
INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width __51___m Fully open x Partly open Partly shaded Fully shaded Sampling reach area _510_m2
Estimated stream depth __135___m Proportion of Reach Represented by Stream High water mark __-___m Morphology Types Surface velocity (at thalweg) __20___msec x Riffle_40_ x Run_50_ x Pool 10 Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___0____ m2 Density of LWD ___-____ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation ___50____ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present Brown Algae
WATER QUALITY Temperature __17___degC Water Odors x NormalNone Sewage Specific Conductance ___-___ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ___-_____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used ____NA_______ Clear x Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other____None____ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 15 Detritus sticks wood coarse plant materials
0 Boulder gt256 mm (10rsquorsquo) 15
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 62 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 8 Sand 006-2 mm (gritty) 0 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Unnamed Tributary STATION HLR-4 STREAM CLASS LAT N 39 13035 (upstream) LONG W 078 05129 (upstream) LAT N 39 13000 (downstream) LONG W 078 05086 (downstream) FORM COMPILED BY Marie Dahl RIVER BASIN Potomac River INVESTIGATORS M Dahl HKepple SRobertson AFisher
DATE 31 May 2008 TIME 5 PM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality 2008
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) YES x NO
rain (steady rain) Air Temperature __22___degC showers (intermittent) Comments _______________________
_0__ cloud cover _0__ ______________________________
x clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area __-___ km2 mi2 Montane Significant springs andor seeps x Valley Marsh or swamp Montane amp Valley Mixture of origins Other ___________________
COMMENTS Snowden Bridge development (upstream) and sewer line
i
j
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence x FieldPasture Industrial Obvious sources Agricultural Other ____________ Construction upstream and adjacent x Residential Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left Forested with rocky banks field across silt fence and 2m tree strip Right Forested with some rocky banks and forest through the highway Indicate the dominant vegetation type(s) and record the dominant species
x Trees x Shrubs x Grasses Forbs Dominant species White Ash and Hackberry
INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width ___38__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area __380__m2
Estimated stream depth __12__cm Proportion of Reach Represented by Stream High water mark __2___m Morphology Types Surface velocity (at thalweg) __46___msec x Riffle__25___ x Run__70___ x Pool__5___ Channelized Yes x No Dam Present x Yes No
LARGE WOODY DEBRIS
LWD _______ m2 Density of LWD _______ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation ___NA____ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present___Algae___
WATER QUALITY Temperature __16___degC Water Odors x NormalNone Sewage Specific Conductance __-____ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ___-_____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used ____NA_____ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells x Other___Shale_______ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 0 Detritus sticks wood coarse plant materials
10 Boulder gt256 mm (10rsquorsquo) 5
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 45 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 30 Sand 006-2 mm (gritty) 0 Marl grey shell
fragments 0
Silt 0004-006 mm 20 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Lick Run STATION HLR-5 STREAM CLASS LAT N 39 127517 (upstream) LONG W 078 05685(upstream) LAT N 39 12784 (downstream) LONG W 078 05631 (downstream) FORM COMPILED BY B Millholland RIVER BASIN Potomac River INVESTIGATORS B Millholland JTovar SRobertson MDahl WBousquet JPangle
DATE 29 May 2008 TIME 1040 AM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project
k
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature __15___degC showers (intermittent) Comments _______________________
_5_ X cloud cover _0__ ______________________________
x clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane Significant springs andor seeps x Valley Marsh or swamp Montane amp Valley Mixture of origins Other ___________________
COMMENTS Lick Run appears to be intermittent and may be dry in Fall 2008
l
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence x FieldPasture Industrial Obvious sources Agricultural Other ____________ Adjacent logging stream bank scour Residential Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left 85 young deciduous forest 15 logged Bank=Soil vegetation and cobbles Right Young deciduous forest on 30 slope Bank= Soil vegetation and cobble Indicate the dominant vegetation type(s) and record the dominant species
x Trees Shrubs Grasses Forbs Dominant species Mixed Deciduous INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width __11__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area 110 m2
Estimated stream depth _009__m Proportion of Reach Represented by Stream High water mark __96___m Morphology Types Surface velocity (at thalweg) __01___msec x Riffle__30___ x Run__5___ x Pool__65___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___0____ m2 Density of LWD ___-____ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __70___ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present__________________________________________________________
WATER QUALITY Temperature __14___degC Water Odors x NormalNone Sewage Specific Conductance __-__ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ___-___ Water Surface Oils Slick Sheen Globs Flecks pH __-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used _____NA______ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other_____None_____
SEDIMENT AND SUBSTRATE
Odors Deposits Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other________________ x Other Slightly Salty Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 0 Detritus sticks wood coarse plant materials
5 Boulder gt256 mm (10rsquorsquo) 0
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 75 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 23 Sand 006-2 mm (gritty) 2 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION McCann Farm STATION HLR-6 STREAM CLASS LAT N 39 13076 (upstream) LONG W 078 06539 (upstream) LAT N 39 13063 (downstream) LONG W 078 06473 (downstream) FORM COMPILED BY MDahl RIVER BASIN Potomac River INVESTIGATORS MDahl JPangle SRobertson W Bousqet BMillholland JTovar H Kepple
DATE 30 May 2008 TIME 1050 AM (start)
PROJECT NAME
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) YES x NO
rain (steady rain) Air Temperature __18___degC showers (intermittent) Comments _______________________
_85_x cloud cover _10__ ______________________________
clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x (Significant springs andor seeps) x ( Valley) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS Multi-habitat sampling with D-frame nets and seine
m
n
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial x No evidence Some evidence x FieldPasture Industrial Obvious sources x Agricultural Other ____________ Minor stream bank scour Residential Local Watershed Erosion x None Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left 35 bank grasses scattered trees and some bushes Right High bank with grasses and scattered trees Indicate the dominant vegetation type(s) and record the dominant species
x Trees Shrubs Grasses Forbs Dominant species Black Walnut and Festuca elatior
INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width __2___m Fully open Partly open x Partly shaded Fully shaded Sampling reach area __200_m2
Estimated stream depth __03_m Proportion of Reach Represented by Stream High water mark __1___m Morphology Types Surface velocity (at thalweg) __166_msec x Riffle__0___ x Run__55___ x Pool__45___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___2____ m2 Density of LWD ___-____ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __20_____ x Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present ____Glyceria (grass)sp__________
WATER QUALITY Temperature __16___degC Water Odors x NormalNone Sewage Specific Conductance ___-___ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ____-____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used ______NA___ Clear x Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other__NONE__ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 0 Detritus sticks wood coarse plant materials
5 Boulder gt256 mm (10rsquorsquo) 0
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 27 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 38 Sand 006-2 mm (gritty) 5 Marl grey shell
fragments 0 (lt1)
Silt 0004-006 mm 25 Clay lt 0004 mm (slick) 5 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Appendix B
WATER QUALITY ANALYSIS THROUGH THE VIRGINIA
STREAM CONDITION INDEX (VSCI)
The Virginia Stream Condition Index (VSCI) relies on eight metrics to categorize stream health According to Burton and Gerritsen (2003 p 1-1) this index ldquohellip can be used as a primary indicator of ecosystem health and can identify impairment with respect to the reference (or natural) conditionrdquo These metrics are numeric measures of a biological communityrsquos species composition diversity ecological niches or tolerance to pollution
Table 13 on the next page describes each VSCI metric lists its formula and provides the best standard value (BSV) for that metric Based on a 100-point scale the BSV is the score that reflects optimum conditions for aquatic life ie the best water quality value possible (DEQ 2006b pp 8 33) The table also explains whether the score increases or decreases with environmental stress
As Table 13 indicates each of the eight VSCI metrics is calculated individually Each formula provides a unitless score based on a 100-point scale with 0 as the worst possible score and 100 as the best Any metric that provides a result above 100 is changed to 100 in order for the index to work properly After the eight metrics are calculated they are summed and averaged to provide a final VSCI score on a 100-point scale
Based on statistical analysis of Virginiarsquos streams and their BMI communities the DEQ (2006b p 8) currently uses the following tiers to interpret VSCI scores
VSCI Scores Aquatic Life Use
(ALI) Tiers
lt 42 severely stressed
42-55 moderately stressed
55-63 unknownfair (gray zone)
63-73 good
gt73 excellent
Section 2 of this report presents the results of calculations for the six BMI sampling sites the research team studied in the Hiatt-Lick Run subwatershed in 2008
o
p
1 Total taxa the number of distinct taxa in the sample measures the overall diversity of the macroinvertebrate assemblage The best standard value (BSV) is 189 Score = 100 x (X189) Total taxa decreases with stress 2 EPT taxa the sum of the distinct taxa in the orders Ephemeroptera (mayflies) Plecoptera (stoneflies) and Trichoptera (caddisflies) These orders are known to be sensitive to pollution The best standard value (BSV) is 117 Score = 100 x (X117) EPT taxa decreases with stress 3 Percent Ephemeroptera the percent abundance of individuals in the sample that belong to the order Ephemeroptera (mayflies) The best standard value (BSV) is 477 Score = 100 x (X477) Percent Ephemeroptera decreases with stress 4 Percent Plecoptera + Trichoptera less Hydropsychidae the percent abundance of individuals in the sample that belong to the orders Plecoptera (stoneflies) and Trichoptera (caddisflies) excluding the pollution-tolerant caddisfly family Hydropsychidae The best standard value (BSV) is 562 Score = 100 x (X562) Percent Plecoptera + Trichoptera less Hydropsychidae decreases with stress 5 Percent Scrapers the percent abundance of individuals in the sample whose primary functional mechanism for feeding is grazing on the substrate or periphyton (ie attached algae and associated material) The best standard value (BSV) is 467 Score = 100 x (X467) Percent scrapers decreases with stress 6 Percent Chironomidae the percent abundance of individuals in the sample that are Chironomidae (non-biting midge) larvae and pupae The best standard value (BSV) is 16 Score = 100 x [(100-X)(100-16)] Percent Chironomidae increases with stress 7 Percent Top Two Dominant the percent abundance of the two most frequent taxa present in the sample The best standard value (BSV) is 328 Score = 100 x [(100-X)(100-328)] The Percent Top 2 Dominant increases with stress 8 Hilsenhoff Biotic Index (HBI) an abundance-weighted average pollution tolerance of all the individuals within a sample keyed to the family level Tolerance ratings range from 1 (highly intolerant) to 10 (highly tolerant) The best standard value (BSV) is 29 Score = 100 x [(10-X)(10-29)] The Hilsenhoff Biotic Index (HBI) increases with stress ______________________________________________________________________________ Table 13 Description of Benthic Macroinvertebrate VSCI Metrics Includes responses (increases decreases) of each VSCI metric to degraded water quality conditions Adapted from Burton and Gerritsen (2003 pp 3-15 3-16 6-2) and DEQ (2008)
q
Appendix C
LABORATORY BENCH SHEETS
Shenandoah Universityrsquos Environmental Studies Department developed the Benthic Macroinvertebrate Laboratory Bench Sheet and VSCI Worksheet to record BMI specimen identifications and their VSCI classifications for each sample
This form is based in part on bench sheets developed by the EPA and Virginia DEQ for recording specimen identifications Additional columns on Shenandoah Universityrsquos forms for relative numbers and VSCI classifications make the calculation and verification of VSCI scores easier
Note the column for taxonomistrsquos initials Specimens were identified by students in Shenandoah Universityrsquos research group and then verified or where necessary changed by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality This external review helps assure the validity of the study Originals of these bench sheets with handwritten taxonomistsrsquo initials will be kept on file at Shenandoah Universityrsquos Environmental Studies Department for five years after the completion of this study
r
s
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-1 STATION NAME Mouth LAT N 39deg 12749rsquo (upstream) LONG W 078deg 04209rsquo (upstream) LAT N 39deg 12729rsquo (downstream) LONG W 078deg 04148rsquo (downstream) FORM COMPILED BY Jeremy Tovar W S Bousquet RIVER BASIN Potomac River INVESTIGATORS Marie Dahl Brandon Millholand J W Pangle Sean Robertson Jeremy Tovar Woodward Bousquet Ben Bousquet
Collection date 21 May 2008 Sheet completion date 12 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Hirudinea 3 33 JT BVW PR 8 SP Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Coenagrionidae 2 22 BVW PR 9 CR Ephemeroptera (mayflies) Caenidae 1 11 JT BVW CG 6 SP Ephemerllidae 4 44 JT BVW CG 2 CR Heptageniidae 5 55 JT BVW SC 3 CG Isonychiidae 1 11 BVW CF 3 CG Plecoptera (stoneflies) Perlidae 14 154 JT BVW PR 2 CR Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Corydalidae 1 11 JT BVW PR 5 CR Coleoptera (beetles) Elmidae 24 264 I JT BVW SC 4 CG Dytiscidae 4 44 JT BVW PR 6 GN Psephenidae 3 33 JT BVW SC 4 CG Diptera (true flies) Tipulidae 3 33 JT BVW SH 4 BU Chironomidae 26 286 JT BVW CG 6 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
t
Other Total Individuals 91 1001 Total Taxa 13
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
u
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-1 STATION NAME Mouth LAT N 39deg 12749rsquo (upstream) LONG W 078deg 04209rsquo (upstream) LAT N 39deg 12729rsquo (downstream) LONG W 078deg 04148rsquo (downstream) FORM COMPILED BY Jeremy Tovar W S Bousquet RIVER BASIN Potomac River INVESTIGATORS Brandon Millholand JW Pangle Jeremy Tovar Heather Kepple
Collection date 10 October 2008 Sheet completion date 12 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Oligochaeta 3 35 JT BVW CG 8 BU Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Coenagrionidae 2 23 JT BVW PR 9 CR Ephemeroptera (mayflies) Heptageniidae 12 140 JT BVW SC 3 CG Isonychiidae 2 23 BVW CF 3 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Hydropsychidae 16 186 JT BVW CF 6 CG Philopotamidae 31 360 JT BVW CF 2 CG Hemiptera (true bugs) (Veliidae) (5) - JT BVW ( ) Megaloptera (dobsonflies etc) Corydalidae 10 116 JT BVW PR 5 CR Coleoptera (beetles) Dytiscidae 1 12 JT BVW PR 6 GN Psephenidae 7 81 JT BVW SC 4 CG Diptera (true flies) Tipulidae 2 23 BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
v
Other Total Individuals 86
(91) 999
Total Taxa 10 (11)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009 Veliidae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
w
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-2 STATION NAME Package Plant LAT N 39deg 12958rsquo (upstream) LONG W 078deg 04900 (upstream) LAT N 39deg 12958rsquo (downstream) LONG W 078deg 04822 (downstream) FORM COMPILED BY JW Pangle W Bousquet RIVER BASIN Potomac River INVESTIGATORS JW Pangle S Robertson J Tovar M Dahl B Millholland W Bousquet
Collection date 29 May 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Plecoptera (stoneflies) Perlidae 11 136 I JP BVW PR 1 CR Trichoptera (caddisflies) Hydropsychidae 10 123 I JP BVW CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 14 173 I JP BVW SC 4 CG Diptera (true flies) Chironomidae 32 395 I JP BVW CG 6 BU Simuliidae 14 173 I JP BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
x
Other Total Individuals 81 1000 Total Taxa 5
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-2 STATION NAME Package Plant LAT N 39deg 12958rsquo (upstream) LONG W 078deg 04900 (upstream) LAT N 39deg 12958rsquo (downstream) LONG W 078deg 04822 (downstream) FORM COMPILED BY JW Pangle W Bousquet RIVER BASIN Potomac River INVESTIGATORS JW Pangle Brandon Millholland Jeremy Tovar Heather Kepple
Collection date 10 October 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Heptageniidae 40 392 I WBJP SC 3 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Hydropsychidae 7 69 I JP WB BVW CF 6 CG Philopotamidae 28 275 I JP WB BVW CF 2 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Corydalidae 6 59 I JP WB BVW PR 5 CR Coleoptera (beetles) Psephenidae 7 69 I JP WB BVW SC 4 CG Elmidae 8 78 I JP WB BVW SC 4 CG Diptera (true flies) Tipulidae 6 59 I JP WB BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
y
z
Other Total Individuals 102 1001 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-3 STATION NAME Rt 664 Bridge LAT N 39deg 12934rsquo (upstream) LONG W 078deg 05019rsquo (upstream) LAT N 39deg 12951rsquo (downstream) LONG W 078deg 04958rsquo (downstream) FORM COMPILED BY S Robertson W Bousquet RIVER BASIN Potomac River INVESTIGATORS SRobertson BMillholland WBousquet MDahl JPangle
Collection date 28 May 2008 Sheet completion date 11 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Leptophlebiidae 1 15 I SGR BVW CG 2 CR Baetidae 1 15 I BVW CG 5 CG Plecoptera (stoneflies) Perlidae 3 46 I SGR BVW PR 1 CR Nemouridae 1 15 I BVW SH 3 CR Trichoptera (caddisflies) Hydropsychidae 5 77 I SGR BVW CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 27 415 I SGR BVW SC 4 CG Psephenidae 2 31 A SGR BVW SC 4 CG Diptera (true flies) Simuliidae 5 77 I SGR BVW CF 6 CG Chironomidae 20 308 I SGR BVW CG 6 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
aa
bb
Other Total Individuals 65 999 Total Taxa 9
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-3 STATION NAME Rt 664 Bridge LAT N 39deg 12934rsquo (upstream) LONG W 078deg 05019rsquo (upstream) LAT N 39deg 12951rsquo (downstream) LONG W 078deg 04958rsquo (downstream) FORM COMPILED BY S Robertson RIVER BASIN Potomac River INVESTIGATORS S Robertson M Dahl A Fisher H Kepple
Collection date 10 Oct 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Heptageniidae 2 20 I SGR SC 3 CG Isonychiidae 2 20 I BVW CF 2 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Philopotamidae 36 367 I SGR CF 2 CG Hydropsychidae 47 480 I SGR CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 2 20 A SGR SC 4 CG Psephenidae 8 82 A SGR SC 4 CG Diptera (true flies) Tabanidae 1 10 I SGR PR 7 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
cc
dd
Other Total Individuals 98 999 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-4 STATION NAME Unnamed Tributary LAT (upstream) N 39deg 13035rsquo LONG (upstream) W 078deg 05129rsquo LAT (downstream) N 39deg 13000rsquo LONG (downstream) W 078deg 5086rsquo FORM COMPILED BY A Fisher M Dahl rev W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson A Fisher H Kepple
Collection date 31 May 2008 Sheet completion date rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Flatworms (Turbellaria) Worms (Oligochaeta) Naididae 1 14 - BVW CG 8 BU Leeches (Hirudinea) Snails (Gastropoda) Clams (Bivalvia) Isopods (Isopoda) Asellidae 3 42 A AF BVW CG 8 SP Scuds (Amphipoda) Crayfish Shrimp (Decapoda) Water Mites (Hydracarina) Dragonflies (Odonata Anisoptera) Damselflies (Odonata Zygoptera) Mayflies (Ephemeroptera) Stoneflies (Plecoptera) Perlidae 2 28 I AF BVW PR 1 CR Caddisflies (Trichoptera) True Bugs (Hemiptera) Corixidae 1 14 I AF BVW MP 9 DV Dobsonflies (Megaloptera) Beetles (Coleoptera) Dytiscidae 1 14 I AF BVW PR 6 GN True Flies (Diptera) Chironomidae 61 847 I AF BVW CG 6 BU Simuliidae 3 42 I AF BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
ee
ff
Other Total Individuals 72 1001 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-4 STATION NAME Unnamed Tributary LAT (upstream) N 39deg 13035rsquo LONG (upstream) W 078deg 05129rsquo LAT (downstream) N 39deg 13000rsquo LONG (downstream) W 078deg 5086rsquo FORM COMPILED BY Andrew G M Fisher W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson A Fisher H Kepple
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Flatworms (Turbellaria) Worms (Oligochaeta) Lumbriculidae 2 32 A AF BVW CG 8 BU Leeches (Hirudinea) Snails (Gastropoda) Lymnaeidae 1 16 A BVW CG 9 GN Clams (Bivalvia) Isopods (Isopoda) Scuds (Amphipoda) Crayfish Shrimp (Decapoda) Cambaridae 1 16 A AF BVW GN 5 GN Water Mites (Hydracarina) Dragonflies (Odonata Anisoptera) Damselflies (Odonata Zygoptera) Calopterygidae 4 63 I BVW PR 7 CL Mayflies (Ephemeroptera) Tricorythidae 1 16 I BVW CG 5 SP Stoneflies (Plecoptera) Caddisflies (Trichoptera) Phryganeidae 4 63 I AF BVW SH 4 CR Hydropsychidae 6 95 I AF BVW CF 6 CG I True Bugs (Hemiptera) Corixidae 8 127 I AF BVW MP 9 DV (Veliidae ) (7) - I AF BVW ( ) Dobsonflies (Megaloptera) Sialidae 3 48 I AF BVW PR 7 SP Corydalidae 2 32 I AF BVW PR 5 CR Beetles (Coleoptera) Haliplidae 1 16 A AF BVW MP 8 GN Dysticidae 7 111 I AF BVW PR 6 GN Hydrophilidae 1 16 A AF BVW PR 8 GN Elmidae 3 48 I BVW SC 4 CG True Flies (Diptera) Chironomidae 8 127 I AF BVW CG 6 BU Simuliidae 7 111 I AF BVW CF 6 CG Tipulidae 4 63 I AF BVW SH 4 GU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
gg
hh
Other Total Individuals 63
(70) 1000
Total Taxa 17 (18)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Veliidae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-5 STATION NAME Lick Run LAT N 39deg 12752rsquo (upstream) LONG W 078deg 05685rsquo (upstream) LAT N 39deg 12784rsquo (downstream) LONG W 078deg 05631rsquo (downstream) FORM COMPILED BY Brandon Millholland W Bousquet RIVER BASIN Potomac River INVESTIGATORS J Pangle S Robertson W Bousquet JTovar M Dahl
Collection date 29 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Gomphidae 1 15 I BVW PR 3 BU Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Leptophlebiidae 11 167 A LBM BVW CG 2 CR Plecoptera (stoneflies) Perlidae 10 152 A LBM BVW PR 1 CR Perlodiae 1 15 A LBM BVW PR 2 CR Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 5 76 A LBM BVW SC 4 CG Elmidae 2 30 I LBM BVW SC 4 CG Diptera (true flies) (Culicidae) (3) - P LMB BVW ( ) Chironomidae 23 348 I LBM BVW CG 6 BU Simuliidae 13 197 I LBM BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
ii
jj
Other Total Individuals 66
(69) 1000
Total Taxa 7 (8)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Culicidae (Diptera) pupae not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl J Pangle S Robertson W Bousquet B Millholland J Tovar H Kepple
Collection date 30 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 1 12 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Asellidae 114 965 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Plecoptera (stoneflies) Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 2 23 A MRD BVW SC 4 CG Diptera (true flies)
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
kk
ll
Other Total Individuals 117 1000 Total Taxa 3
Notes Subsample taken 29 October 2009 All taxonomic determinations by MRD were confirmed by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson K McCann A Fisher H Kepple A Barrett
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 2 17 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Sphaeriidae 2 17 I MRD BVW CF 8 BU Isopoda (isopods) Asellidae 99 853 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Calopterygidae 2 17 A MRD BVW PR 7 CL Ephemeroptera (mayflies) Leptophlebiidae 2 17 I MRD BVW CG 2 CR Heptageniidae 4 34 A MRD BVW SC 3 CG Plecoptera (stoneflies) Perlidae 1 09 A MRD BVW PR 1 CR Trichoptera (caddisflies) Hemiptera (true bugs) (Gerridae) (1) - A MRD BVW ( ) Corixidae 1 09 A MRD BVW PR 5 CR Megaloptera (dobsonflies etc) Coleoptera (beetles) Dryopidae 1 09 A MRD BVW SC 5 CG Diptera (true flies) Tipulidae 2 17 I BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
mm
nn
Other Total Individuals 116
(117) 999
Total Taxa 10 (11)
Notes Subsample taken 6 November 2008 Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009 Gerridae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
- Disclaimer ii
- Title Page iii
- Acknowledgements v
- Table of Contents vii
- SECTION 2 RESULTS DATA TABLES 7
- OVERVIEW AND FINDINGS
- Introduction
- RESULTS DATA TABLES
- REFERENCES
- FIELD DATA SHEETS
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- SubstrateType
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- WATER QUALITY ANALYSIS
- THROUGH THE VIRGINIA
- STREAM CONDITION INDEX (VSCI)
- LABORATORY BENCH SHEETS
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
Figure 1 Sampling Sites in the Hiatt-Lick Run Subwatershed of Opequon Creek Frederick County Virginia Scale 1 centimeter = 500 meters
HLR-1 (Mouth) measures water quality at the lowest point of the subwatershed as Hiatt Run flows into Opequon Creek Forests cover the immediate riparian zones on both sides of Hiatt Run This site will be used in future years by the DEQ for BMI monitoring HLR-2 (Package Plant) is situated on Hiatt Run just below the outflow pipe of an outdated package plant that formerly treated sewage from the Jordan Springs area During the study period the package plant was taken off-line and a pumping station was constructed The Frederick-Winchester Service Authority now treats this sewage at its Opequon Water Reclamation Facility HLR-3 (Route 664 Bridge) was chosen because it is located upstream from the package plant but downstream of the confluence of Hiatt Run and Lick Run HLR-4 (Unnamed Tributary) is located downstream of the Snowden Bridge residential development which has been under construction The area immediately upstream of this sampling site was being graded by heavy equipment during the May 2008 sampling time HLR-5 (Lick Run) the researchers learned dries up in late summer It had a modest but constant flow during when the May 2008 sample was taken HLR-6 (McCann Farm) represents the headwater areas of the Hiatt-Lick Run subwatershed of Opequon Creek The McCanns who graze cattle in their pastures that surround Hiatt Run here have been working with the Potomac Conservancy to implement Best Management Practices
2
(BMPs) to improve water quality These practices include fencing cattle out of the stream and spring runs and planting more trees in the riparian zone
Methods and Analysis
The research team established a sampling plan and then sampled according to methods the DEQ developed for the VSCI Using a 1 meter x 1 meter seine samples from 2m2 of substrate in riffle zones were to be collected twice at each site once in May 2008 and once in October 2008 At five of the six sites stream characteristics permitted the standard single-habitat sampling procedure At the McCann Farm (Site HLR-6) researchers needed to use multi-habitat sampling See Barbour et al (1999 pp 7-3 ff) for further descriptions of these two methods
All six sites were sampled in May 2008 Low-water conditions in October 2008 prevented the Shenandoah University group from collecting BMIs at Lick Run (HLR-5) that month but the other five sites were sampled successfully As a result the research team obtained eleven samples for analysis in this study Samples were preserved in ethanol for subsequent identification
DEQ procedures require subsampling when the number of BMIs in a given sample exceeds 100 All eleven samples in this study met that criterion Consequently researchers used a Caton tray to produce random subsamples of 90 to 110 specimens from each sample During the analysis that followed some specimens in the subsamples were rejected either because they turned out to be terrestrial species or because they were in the wrong life stage (eg pupae) In most cases the researchers identified specimens to the family level Primary taxonomic references were A Guide to Common Freshwater Invertebrates of North America (Voshell 2002) Fresh-Water Invertebrates of the United States (Pennak 1953) and An Introduction to the Aquatic Insects of North America (Merritt and Cummins 1996) DEQ Regional Aquatic Biologist Billy van Wart reviewed verified and where necessary corrected the identifications
The investigators then used the Virginia Stream Condition Index (VSCI) to produce water quality scores based on the BMIs collected A VSCI score is based on eight different measurement formulas called metrics Each metric is a numeric measure of a biological communityrsquos species composition diversity ecological niches or tolerance to pollution When combined into a single multimetric VSCI score the response of the benthic community to water conditions can be evaluated For further information and worksheets see Appendices B and C respectively
Findings
Table 1 presents the VSCI scores for the eleven samples collected in the Hiatt-Lick Run subwatershed in 2008 Since a sample year consists of two samples the mean scores for each site for the entire year are also included As the table shows
bull None of the 11 samples was rated excellent bull Only 1 of the 11 samples was rated as good bull Only 2 of the 11 samples were rated fair (ie in the ldquogrey zonerdquo between impaired and
unimpaired) bull The remaining 8 samples were in either moderately stressed or severely stressed
condition
3
Site Name and Code Sampling
Date of
TaxaVCSI Score
Water Quality Rating
Mouth (HLR-1) 21 May 2008 13 571 Fair (Gray Zone)
Mouth (HLR-1) 10 Oct 2008 10 608 Fair (Gray Zone)
site average for 2008 590 Fair (Gray Zone)
Package Plant (HLR-2) 29 May 2008 5 370 Severely Stressed
Package Plant (HLR-2) 10 Oct 2008 7 674 Good
site average for 2008 522 Moderately Stressed
Route 664 Bridge (HLR-3) 28 May 2008 9 485 Moderately Stressed
Route 664 Bridge (HLR-3) 10 Oct 2008 7 469 Moderately Stressed
site average for 2008 477 Moderately Stressed
Unnamed Tributary (HLR-4) 31 May 2008 7 173 Severely Stressed
Unnamed Tributary (HLR-4) 10 Oct 2008 17 476 Moderately Stressed
site average for 2008 325 Severely Stressed
Upper Lick Run (HLR-5) 29 May 2008 7 455 Moderately Stressed
site average for 2008 455 Moderately Stressed
McCann Farm (HLR-6) 30 May 2008 3 190 Severely Stressed
McCann Farm (HLR-6) 10 Oct 2008 10 316 Severely Stressed
site average for 2008 253 Severely Stressed
Summary 6 sites 11 samples
36 BMI taxa
VSCI = Virginia Stream Condition Index see Burton and Gerritsen (2003) and Virginia Department of Environmental Quality (2006b and 2008) Table 1 Summary Table of Virginia Stream Condition Index (VSCI) Scores and Water Quality Ratings for Sites in the Hiatt-Lick Run Subwatershed of Opequon Creek Frederick County Virginia 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
4
Improvements in water quality ratings at three sites between May and October suggest that BMPs and other changes may already be having some positive effects on water quality At the Package Plant (Site HLR-2) the VSCI score rose dramatically from 370 (severely stressed) to 674 (good) The small-scale sewage treatment plant as noted above was taken off-line during this period Similarly water quality at the Unnamed Tributary (HLR-4) increased from 173 (severely stressed) to 476 (moderately stressed) Adjacent construction was completed over the summer and grass was planted on the property Management practices on the McCann Farm (HLR-6) may be responsible for improving conditions for aquatic life in Hiatt Runrsquos headwaters Even though the water quality was still rated as severely stressed in October the VSCI score rose from 190 to 316 at this sampling site Further studies are needed to determine whether the changes noted in 2008 persist into the future In 2003 the DEQ began a Total Maximum Daily Load (TMDL) process for Opequon Creek as required by the Water Quality Act of 1987 The TMDL approach establishes a ldquopollution budgetrdquo for each impaired stream for each type of impairment and for each responsible pollutant In conjunction with the DEQ and the Virginia Department of Conservation and Recreation (DCR) Virginia Polytechnic Institutersquos Department of Biosystems Engineering developed a TMDL implementation plan (Opequon Creek IP Steering Committee et al 2006) Monitoring the BMI communities of Opequon Creek and its tributaries is essential to evaluating the progress of the proposed water quality improvement projects
Future Research The relatively small Hiatt-Lick Run subwatershed provides several case studies that can help assess the effectiveness of various water quality management practices not only in Opequon Creek but also in other watersheds in the Chesapeake Bay region and beyond In supporting that goal this study makes two principal contributions 1 It provides baseline data to measure the improvement or degradation of the Hiatt-Lick Run section of the Opequon Creek watershed and 2 It establishes a monitoring framework for the long-term biological monitoring of water quality and the evaluation of best management practices (BMPs) This framework consists of
bull Sampling Sites ndash six stream reaches in the Hiatt-Lick Run subwatershed selected as sampling sites according to EPA and DEQ specifications
bull Data Collection Protocols ndash based on the EPArsquos Rapid Bioassessment Protocols (RBPs)
(Barbour et al 1999) and the DEQrsquos Virginia Stream Index (VSCI) (Burton and Gerritsen 2003 DEQ 2006b and 2008) and
bull Analytical Procedures ndash contained in the Virginia Stream Condition Index (VSCI see
Burton and Gerritsen 2003 DEQ 2006b pp 8 and 33 and DEQ 2008 pp 29-34)
5
6
Section 2
RESULTS DATA TABLES
Site HLR-1 Mouth
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 13 189 6872 EPT taxa 5 117 4273 Ephemeroptera 121 477 2544 Plec +Tric less Hydropsych 154 562 2745 Scrapers 352 467 7536 Chironomidae 286 16 7267 Top 2 Dominant 550 328 6708 Hilsenhoff Biotic Index (HBI) 45 29 775VSCI Score 571
According to the DEQrsquos water quality standards aquatic life at this site is fair (gray zone) See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 2 VSCI Scores for Mouth (HLR-1) Site in Hiatt-Lick Run Sampled on 21 May 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 10 189 5292 EPT taxa 4 117 3423 Ephemeroptera 163 477 3424 Plec +Tric less Hydropsych 360 562 6415 Scrapers 221 467 4736 Chironomidae 00 16 10007 Top 2 Dominant 546 328 6768 Hilsenhoff Biotic Index (HBI) 39 29 859VSCI Score 608
According to the DEQrsquos water quality standards aquatic life at this site is fair (gray zone) See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 3 VSCI Scores for Mouth (HLR-1) Site in Hiatt-Lick Run Sampled 10 October 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
7
Site HLR-2 Package Plant
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 5 189 2642 EPT taxa 2 117 1713 Ephemeroptera 00 477 004 Plec +Tric less Hydropsych 110 562 1965 Scrapers 173 467 3706 Chironomidae 395 16 6157 Top 2 Dominant 568 328 6438 Hilsenhoff Biotic Index (HBI) 50 29 704VSCI Score 370
According to the DEQrsquos water quality standards aquatic life at this site is severely stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 4 VSCI Scores for Package Plant (HLR-2) Site in Hiatt-Lick Run Sampled 29 May 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 7 189 3702 EPT taxa 34 117 2563 Ephemeroptera 392 477 8214 Plec +Tric less Hydropsych 275 562 4895 Scrapers 539 467 10006 Chironomidae 00 16 10007 Top 2 Dominant 667 328 4968 Hilsenhoff Biotic Index (HBI) 32 29 958VSCI Score 674
According to the DEQrsquos water quality standards aquatic life at this site is good See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 5 VSCI Scores for Package Plant (HLR-2) Site in Hiatt-Lick Run Sampled 10 October 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
8
Site HLR-3 Route 664 Bridge
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 9 189 4762 EPT taxa 5 117 4273 Ephemeroptera 30 477 634 Plec +Tric less Hydropsych 61 562 1095 Scrapers 446 467 9556 Chironomidae 308 16 7037 Top 2 Dominant 723 328 4128 Hilsenhoff Biotic Index (HBI) 48 29 732VSCI Score 485
According to the DEQrsquos water quality standards aquatic life at this site is moderately stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 6 VSCI Scores for Route 664 Bridge (HLR-3) Site in Hiatt-Lick Run Sampled 28 May 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 7 189 3702 EPT taxa 4 117 3423 Ephemeroptera 40 477 844 Plec +Tric less Hydropsych 367 562 6535 Scrapers 122 467 2616 Chironomidae 00 16 10007 Top 2 Dominant 847 328 2288 Hilsenhoff Biotic Index (HBI) 42 29 817VSCI Score 469
According to the DEQrsquos water quality standards aquatic life at this site is moderately stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 7 VSCI Scores for Route 664 Bridge (HLR-3) Site in Hiatt-Lick Run Sampled 10 October 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
9
Site HLR-4 Unnamed Tributary
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 7 189 3702 EPT taxa 1 117 853 Ephemeroptera 00 477 004 Plec +Tric less Hydropsych 28 562 495 Scrapers 00 467 006 Chironomidae 847 16 1557 Top 2 Dominant 889 328 1658 Hilsenhoff Biotic Index (HBI) 60 29 563VSCI Score 173
According to the DEQrsquos water quality standards aquatic life at this site is severely stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 8 VSCI Scores for Unnamed Tributary (HLR-4) Site in Hiatt-Lick Run Sampled 31 May 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 17 189 8992 EPT taxa 3 117 2563 Ephemeroptera 16 477 344 Plec +Tric less Hydropsych 63 562 1125 Scrapers 48 467 1036 Chironomidae 127 16 8877 Top 2 Dominant 254 328 10008 Hilsenhoff Biotic Index (HBI) 63 29 521VSCI Score 476
According to the DEQrsquos water quality standards aquatic life at this site is moderately stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 9 VSCI Scores for Unnamed Tributary (HLR-4) Site in Hiatt-Lick Run Sampled 10 October 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
10
Site HLR-5 Lick Run
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 7 189 3702 EPT taxa 3 117 2563 Ephemeroptera 167 477 3504 Plec +Tric less Hydropsych 167 562 2975 Scrapers 106 467 2276 Chironomidae 348 16 6637 Top 2 Dominant 545 328 6778 Hilsenhoff Biotic Index (HBI) 43 29 803VSCI Score 455
According to the DEQrsquos water quality standards aquatic life at this site is moderately stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 10 VSCI Scores for Lick Run (HLR-5) Site in Hiatt-Lick Run Sampled 29 May 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
Note
Water conditions at the Lick Run (HLR-5) site were too low in October 2008 to permit a sample to be taken
11
Site HLR-6 McCann Farm
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 3 189 1592 EPT taxa 0 117 003 Ephemeroptera 00 477 004 Plec +Tric less Hydropsych 00 562 005 Scrapers 23 467 496 Chironomidae 00 16 10007 Top 2 Dominant 988 328 188 Hilsenhoff Biotic Index (HBI) 79 29 296VSCI Score 190
According to the DEQrsquos water quality standards aquatic life at this site is severely stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 11 VSCI Scores for McCann Farm (HLR-6) Site in Hiatt-Lick Run Sampled 30 May 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 10 189 5292 EPT taxa 3 117 2563 Ephemeroptera 51 477 1074 Plec +Tric less Hydropsych 09 562 165 Scrapers 43 467 926 Chironomidae 00 16 10007 Top 2 Dominant 887 328 1688 Hilsenhoff Biotic Index (HBI) 75 29 352VSCI Score 315
According to the DEQrsquos water quality standards aquatic life at this site is severely stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 12 VSCI Scores for McCann Farm (HLR-6) Site in Hiatt-Lick Run Sampled 10 October 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
12
REFERENCES
Barbour Michael T Jeroen Gerritsen Blaine D Snyder and James B Stribling 1999 Rapid bioassessment protocols for use in streams and wadeable rivers periphyton benthic macroinvertebrates and fish Second edition EPA 841-B-99-002 Washington DC US Environmental Protection Agency Office of Water Available online at httpwwwepagovOWOWmonitoringbioasspshtml Burton June and Jeroen Gerritsen 2003 A stream condition index for Virginia non-coastal streams Prepared for the US EPA Office of Science and Technology US EPA Region 3 Environmental Services Division and Virginia Department of Environmental Quality Owings Mills Maryland Tetra Tech Inc Available online at httpwwwdeqvirginiagovwatermonitoringpdf vastrmconpdf Hessler Rachel A Andrea R Martin Heidi D Ruggeri William B Seymour Jr and Woodward S Bousquet 2006 Water quality assessment using benthic macroinvertebrates A study and compilation Winchester Virginia Environmental Studies Program Shenandoah University Occasional publication no 10 Igoe Robert 2006 DEQ identifies nine impaired streams in Clarke Frederick The Winchester Star 15 July B1 Jackson Charlie 2005 Survey targets Opequon cleanup The Winchester Star 19 August B1 Johnston Elizabeth A and Woodward S Bousquet 2003 What happens when it rains A study of water quality in Abrams Creek and Town Run Winchester and Frederick County Virginia Winchester Virginia Environmental Studies Program Shenandoah University Occasional publication no7 Merritt Richard W and Kenneth W Cummins 1996 An introduction to the aquatic insects of North America Dubuque Iowa KendallHunt Publishing Company Opequon Creek IP Steering Committee Virginia Tech Department of Biological Systems Engineering and Center for TMDL and Watershed Studies University of Virginia Institute for Environmental Negotiation and West Virginia University Division of Resource Management 2006 Opequon Creek watershed TMDL implementation plan Blacksburg Virginia Virginia Tech Department of Biological Systems Engineering Available online at httpwwwtmdlbsevteduoutreachC82 Pennak Robert W 1953 Fresh-water invertebrates of the United States New York New York The Ronald Press Company Rosenburg David M and Vincent H Resh 1993 Freshwater biomonitoring and benthic macroinvertebrates New York New York Chapman amp Hall Shenandoah University Public Relations Office 2002 Data collected by SU and its partners helps identify impaired water Winchester Virginia Available online at httpwwwsuedutemp_ newscfmurlnum=32 Thorp James H and Alan P Covich 1991 Ecology and classification of North American freshwater invertebrates San Diego California Academic Press Inc
13
US Environmental Protection Agency 1997 Volunteer stream monitoring a methods manual EPA 841-B-97-003 Washington DC US Environmental Protection Agency Office of Water Van Meter Val 2003 Opequon Creek pollution limits to be established The Winchester Star 4 April B1 Virginia Department of Environmental Quality 2006a Virginia 305(b)303(d) water quality integrated report to Congress and the EPA Administrator for the period January 1 2000 to December 31 2004 Richmond Virginia Virginia Department of Environmental Quality Available online at httpwwwdeqvirginiagovwqair2006html __________ 2006b Using probabilistic data to validate the non-coastal Virginia Stream Condition Index (draft) VDEQ technical bulletin WQA2006-001 Virginia Department of Environmental Quality Available online at httpwwwloudounwatershedorgstream_assessmentreference_documents VA_DEQ_SCI_Validationpdf __________ 2008 Biological monitoring program quality assurance project plan for wadeable streams and rivers Richmond Virginia Virginia Department of Environmental Quality Update available online at httpwwwdeqstatevausbiomonitoringdocumentsBioMonQAPP_13Aug2008pdf Virginia Tech Aquatic Entomology Laboratory 2005 Taxa dictionary [Excel file] Blacksburg Virginia Virginia Tech Department of Biological Systems Engineering 2003a Opequon watershed TMDLs for benthic impairments Abrams Creek and Lower Opequon Creek Frederick and Clarke Counties Virginia Prepared for the Virginia Department of Environmental Quality and Virginia Department of Conservation and Recreation Blacksburg Virginia Available online at httpwwwdeqstate vaustmdlapptmdlsshenrvrabropebcpdf Voshell J Reese Jr 2002 A guide to common freshwater invertebrates of North America Blacksburg Virginia The McDonald amp Woodward Publishing Company
14
APPENDICES
15
16
a
Appendix A
FIELD DATA SHEETS
This appendix presents a Site Characterization Field Data Sheet for each of the six sampling reaches established on Hiatt Run and Lick Run for this study They were filled out during the sampling runs made in May 2008
These forms were adapted by Shenandoah University from forms used by the EPA and Virginia DEQ for recording characteristics of sampling sites
b
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Mouth STATION _HLR-1_____ STREAM CLASS LAT N 39 12749 (upstream) LONG W 078 04209(upstream) LAT N 39 12729 (downstream) LONG W 078 04148 (downstream) FORM COMPILED BY Jeremy Tovar RIVER BASIN Potomac River INVESTIGATORS JPangle B Millholland MDahl SRobertson W Bousquet JTovar BBousquet
DATE May 21 2008_________ TIME_250 PM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature __24___degC showers (intermittent) Comments _______________________
___ cloud cover _0_ ______________________________
X clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x ( Significant springs andor seeps) x (Valley ) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS
c
d
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence x FieldPasture Industrial Obvious sources Agricultural Other ____________ Stream scour steep slope erosion x Residential Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left Field and young thin deciduous forest 100 Bank=Grass cobble and shrub Right 90 maturing deciduous forest 45 shale slope Bank=Bedrock soil and herbs Indicate the dominant vegetation type(s) and record the dominant species
x Trees Shrubs x (Grasses) Forbs Dominant species Oak species and mix deciduous
INSTREAM FEATURES
Measured reach length ___100__m Canopy Cover Estimated stream width _35__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area __350_m2
Estimated stream depth _008_m Proportion of Reach Represented by Stream High water mark _15_m Morphology Types Surface velocity (at thalweg) _05__msec x Riffle__75___ x Run__20___ x Pool__5___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___00__ m2 Density of LWD _-_ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __15_____ x Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present Algae
WATER QUALITY Temperature __19___degC Water Odors x NormalNone Sewage Specific Conductance - Petroleum Chemical Fishy Other___________________ Dissolved Oxygen - Water Surface Oils Slick Sheen Globs Flecks pH __-_ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used _____NA________ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic x None Relict shells Other______None_____ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 8 Detritus sticks wood coarse plant materials
0 Boulder gt256 mm (10rsquorsquo) 0
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 51 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 40 Sand 006-2 mm (gritty) 1 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University
SITE CHARACTERIZATION FIELD DATA SHEET FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Package Plant STATION HLR-2 STREAM CLASS LAT N 39 129580 (upstream) LONG W 078 04900 (upstream) LAT N 39 129581 (downstream) LONG W 078 04822 (downstream) FORM COMPILED BY JW Pangle RIVER BASIN Potomac River INVESTIGATORS JPangle JTovar MDahl B Millholland SRobertson W Bousquet
DATE__19 May 2008_ TIME__1240__ PM
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature _____degC showers (intermittent) Comments __Sunny Day_____________
_5__ cloud cover _0__ ______________________________
x clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x (Significant springs andor seeps) x (Valley) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS
e
f
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence FieldPasture Industrial Obvious sources Agricultural Other ____________ __Upstream Construction and Stream bank Scour Residential __________________________________ Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left Deciduous forest with thick herb and shrub layers bank= vegetation and rocks Right Deciduous forest with thick herb and shrub layers bank=vegetation soil and rocks Indicate the dominant vegetation type(s) and record the dominant species
x Trees x Shrubs Grasses Forbs Dominant species Black Walnut White Ash Hackberry and American Sycamore
INSTREAM FEATURES
Measured reach length 100 m Canopy Cover Estimated stream width _35__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area __350_m2
Estimated stream depth __15__m Proportion of Reach Represented by Stream High water mark __22___m Morphology Types Surface velocity (at thalweg) _035__msec x Riffle__30___ x Run__30___ x Pool__40___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___10____ m2 Density of LWD _______ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __30___ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present__________________________________________________________
WATER QUALITY Temperature __18___degC Water Odors x NormalNone Sewage Specific Conductance __-____ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ____-____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used _____NA____ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other___None_____ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach SubstrateType Characteristic
Composition in Sampling Reach
Bedrock 3 Detritus sticks wood coarse plant materials
2 Boulder gt256 mm (10rsquorsquo) 5
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 70 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 22 Sand 006-2 mm (gritty) 0 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiattrsquos Run LOCATION Rt 664 Bridge STATION HLR-3 STREAM CLASS LAT N 39 12934 (upstream) LONG W 078 05019 (upstream) LAT N 39 12958 (downstream) LONG W 078 04958 (downstream) FORM COMPILED BY Sean Robertson RIVER BASIN Potomac River INVESTIGATORS SRobertson BMillholland WBousquet MDahl JPangle
DATE 28 May 2008 TIME 1127 AM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality 2008
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature __14___degC showers (intermittent) x Trace Comments _______________________
_65 x cloud cover ___ ______________________________
x clearsunny __________________________________
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x (Significant springs andor seeps) x (Valley) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS
g
h
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence Some evidence x FieldPasture Industrial x Obvious sources Agricultural x Other Old Package Plant Downstream x Residential Bridge and Road Power line and Pump station Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left 70 field 30 shrubs and deciduous trees Bank=Soil and vegetation Right Maturing deciduous forest steep slope Bank=Shale Bedrock Indicate the dominant vegetation type(s) and record the dominant species
x Trees x Shrubs x Grasses Forbs Dominant species Sycamore and Red Oaks
INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width __51___m Fully open x Partly open Partly shaded Fully shaded Sampling reach area _510_m2
Estimated stream depth __135___m Proportion of Reach Represented by Stream High water mark __-___m Morphology Types Surface velocity (at thalweg) __20___msec x Riffle_40_ x Run_50_ x Pool 10 Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___0____ m2 Density of LWD ___-____ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation ___50____ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present Brown Algae
WATER QUALITY Temperature __17___degC Water Odors x NormalNone Sewage Specific Conductance ___-___ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ___-_____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used ____NA_______ Clear x Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other____None____ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 15 Detritus sticks wood coarse plant materials
0 Boulder gt256 mm (10rsquorsquo) 15
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 62 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 8 Sand 006-2 mm (gritty) 0 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Unnamed Tributary STATION HLR-4 STREAM CLASS LAT N 39 13035 (upstream) LONG W 078 05129 (upstream) LAT N 39 13000 (downstream) LONG W 078 05086 (downstream) FORM COMPILED BY Marie Dahl RIVER BASIN Potomac River INVESTIGATORS M Dahl HKepple SRobertson AFisher
DATE 31 May 2008 TIME 5 PM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality 2008
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) YES x NO
rain (steady rain) Air Temperature __22___degC showers (intermittent) Comments _______________________
_0__ cloud cover _0__ ______________________________
x clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area __-___ km2 mi2 Montane Significant springs andor seeps x Valley Marsh or swamp Montane amp Valley Mixture of origins Other ___________________
COMMENTS Snowden Bridge development (upstream) and sewer line
i
j
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence x FieldPasture Industrial Obvious sources Agricultural Other ____________ Construction upstream and adjacent x Residential Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left Forested with rocky banks field across silt fence and 2m tree strip Right Forested with some rocky banks and forest through the highway Indicate the dominant vegetation type(s) and record the dominant species
x Trees x Shrubs x Grasses Forbs Dominant species White Ash and Hackberry
INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width ___38__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area __380__m2
Estimated stream depth __12__cm Proportion of Reach Represented by Stream High water mark __2___m Morphology Types Surface velocity (at thalweg) __46___msec x Riffle__25___ x Run__70___ x Pool__5___ Channelized Yes x No Dam Present x Yes No
LARGE WOODY DEBRIS
LWD _______ m2 Density of LWD _______ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation ___NA____ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present___Algae___
WATER QUALITY Temperature __16___degC Water Odors x NormalNone Sewage Specific Conductance __-____ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ___-_____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used ____NA_____ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells x Other___Shale_______ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 0 Detritus sticks wood coarse plant materials
10 Boulder gt256 mm (10rsquorsquo) 5
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 45 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 30 Sand 006-2 mm (gritty) 0 Marl grey shell
fragments 0
Silt 0004-006 mm 20 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Lick Run STATION HLR-5 STREAM CLASS LAT N 39 127517 (upstream) LONG W 078 05685(upstream) LAT N 39 12784 (downstream) LONG W 078 05631 (downstream) FORM COMPILED BY B Millholland RIVER BASIN Potomac River INVESTIGATORS B Millholland JTovar SRobertson MDahl WBousquet JPangle
DATE 29 May 2008 TIME 1040 AM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project
k
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature __15___degC showers (intermittent) Comments _______________________
_5_ X cloud cover _0__ ______________________________
x clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane Significant springs andor seeps x Valley Marsh or swamp Montane amp Valley Mixture of origins Other ___________________
COMMENTS Lick Run appears to be intermittent and may be dry in Fall 2008
l
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence x FieldPasture Industrial Obvious sources Agricultural Other ____________ Adjacent logging stream bank scour Residential Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left 85 young deciduous forest 15 logged Bank=Soil vegetation and cobbles Right Young deciduous forest on 30 slope Bank= Soil vegetation and cobble Indicate the dominant vegetation type(s) and record the dominant species
x Trees Shrubs Grasses Forbs Dominant species Mixed Deciduous INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width __11__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area 110 m2
Estimated stream depth _009__m Proportion of Reach Represented by Stream High water mark __96___m Morphology Types Surface velocity (at thalweg) __01___msec x Riffle__30___ x Run__5___ x Pool__65___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___0____ m2 Density of LWD ___-____ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __70___ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present__________________________________________________________
WATER QUALITY Temperature __14___degC Water Odors x NormalNone Sewage Specific Conductance __-__ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ___-___ Water Surface Oils Slick Sheen Globs Flecks pH __-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used _____NA______ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other_____None_____
SEDIMENT AND SUBSTRATE
Odors Deposits Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other________________ x Other Slightly Salty Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 0 Detritus sticks wood coarse plant materials
5 Boulder gt256 mm (10rsquorsquo) 0
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 75 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 23 Sand 006-2 mm (gritty) 2 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION McCann Farm STATION HLR-6 STREAM CLASS LAT N 39 13076 (upstream) LONG W 078 06539 (upstream) LAT N 39 13063 (downstream) LONG W 078 06473 (downstream) FORM COMPILED BY MDahl RIVER BASIN Potomac River INVESTIGATORS MDahl JPangle SRobertson W Bousqet BMillholland JTovar H Kepple
DATE 30 May 2008 TIME 1050 AM (start)
PROJECT NAME
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) YES x NO
rain (steady rain) Air Temperature __18___degC showers (intermittent) Comments _______________________
_85_x cloud cover _10__ ______________________________
clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x (Significant springs andor seeps) x ( Valley) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS Multi-habitat sampling with D-frame nets and seine
m
n
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial x No evidence Some evidence x FieldPasture Industrial Obvious sources x Agricultural Other ____________ Minor stream bank scour Residential Local Watershed Erosion x None Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left 35 bank grasses scattered trees and some bushes Right High bank with grasses and scattered trees Indicate the dominant vegetation type(s) and record the dominant species
x Trees Shrubs Grasses Forbs Dominant species Black Walnut and Festuca elatior
INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width __2___m Fully open Partly open x Partly shaded Fully shaded Sampling reach area __200_m2
Estimated stream depth __03_m Proportion of Reach Represented by Stream High water mark __1___m Morphology Types Surface velocity (at thalweg) __166_msec x Riffle__0___ x Run__55___ x Pool__45___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___2____ m2 Density of LWD ___-____ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __20_____ x Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present ____Glyceria (grass)sp__________
WATER QUALITY Temperature __16___degC Water Odors x NormalNone Sewage Specific Conductance ___-___ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ____-____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used ______NA___ Clear x Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other__NONE__ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 0 Detritus sticks wood coarse plant materials
5 Boulder gt256 mm (10rsquorsquo) 0
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 27 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 38 Sand 006-2 mm (gritty) 5 Marl grey shell
fragments 0 (lt1)
Silt 0004-006 mm 25 Clay lt 0004 mm (slick) 5 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Appendix B
WATER QUALITY ANALYSIS THROUGH THE VIRGINIA
STREAM CONDITION INDEX (VSCI)
The Virginia Stream Condition Index (VSCI) relies on eight metrics to categorize stream health According to Burton and Gerritsen (2003 p 1-1) this index ldquohellip can be used as a primary indicator of ecosystem health and can identify impairment with respect to the reference (or natural) conditionrdquo These metrics are numeric measures of a biological communityrsquos species composition diversity ecological niches or tolerance to pollution
Table 13 on the next page describes each VSCI metric lists its formula and provides the best standard value (BSV) for that metric Based on a 100-point scale the BSV is the score that reflects optimum conditions for aquatic life ie the best water quality value possible (DEQ 2006b pp 8 33) The table also explains whether the score increases or decreases with environmental stress
As Table 13 indicates each of the eight VSCI metrics is calculated individually Each formula provides a unitless score based on a 100-point scale with 0 as the worst possible score and 100 as the best Any metric that provides a result above 100 is changed to 100 in order for the index to work properly After the eight metrics are calculated they are summed and averaged to provide a final VSCI score on a 100-point scale
Based on statistical analysis of Virginiarsquos streams and their BMI communities the DEQ (2006b p 8) currently uses the following tiers to interpret VSCI scores
VSCI Scores Aquatic Life Use
(ALI) Tiers
lt 42 severely stressed
42-55 moderately stressed
55-63 unknownfair (gray zone)
63-73 good
gt73 excellent
Section 2 of this report presents the results of calculations for the six BMI sampling sites the research team studied in the Hiatt-Lick Run subwatershed in 2008
o
p
1 Total taxa the number of distinct taxa in the sample measures the overall diversity of the macroinvertebrate assemblage The best standard value (BSV) is 189 Score = 100 x (X189) Total taxa decreases with stress 2 EPT taxa the sum of the distinct taxa in the orders Ephemeroptera (mayflies) Plecoptera (stoneflies) and Trichoptera (caddisflies) These orders are known to be sensitive to pollution The best standard value (BSV) is 117 Score = 100 x (X117) EPT taxa decreases with stress 3 Percent Ephemeroptera the percent abundance of individuals in the sample that belong to the order Ephemeroptera (mayflies) The best standard value (BSV) is 477 Score = 100 x (X477) Percent Ephemeroptera decreases with stress 4 Percent Plecoptera + Trichoptera less Hydropsychidae the percent abundance of individuals in the sample that belong to the orders Plecoptera (stoneflies) and Trichoptera (caddisflies) excluding the pollution-tolerant caddisfly family Hydropsychidae The best standard value (BSV) is 562 Score = 100 x (X562) Percent Plecoptera + Trichoptera less Hydropsychidae decreases with stress 5 Percent Scrapers the percent abundance of individuals in the sample whose primary functional mechanism for feeding is grazing on the substrate or periphyton (ie attached algae and associated material) The best standard value (BSV) is 467 Score = 100 x (X467) Percent scrapers decreases with stress 6 Percent Chironomidae the percent abundance of individuals in the sample that are Chironomidae (non-biting midge) larvae and pupae The best standard value (BSV) is 16 Score = 100 x [(100-X)(100-16)] Percent Chironomidae increases with stress 7 Percent Top Two Dominant the percent abundance of the two most frequent taxa present in the sample The best standard value (BSV) is 328 Score = 100 x [(100-X)(100-328)] The Percent Top 2 Dominant increases with stress 8 Hilsenhoff Biotic Index (HBI) an abundance-weighted average pollution tolerance of all the individuals within a sample keyed to the family level Tolerance ratings range from 1 (highly intolerant) to 10 (highly tolerant) The best standard value (BSV) is 29 Score = 100 x [(10-X)(10-29)] The Hilsenhoff Biotic Index (HBI) increases with stress ______________________________________________________________________________ Table 13 Description of Benthic Macroinvertebrate VSCI Metrics Includes responses (increases decreases) of each VSCI metric to degraded water quality conditions Adapted from Burton and Gerritsen (2003 pp 3-15 3-16 6-2) and DEQ (2008)
q
Appendix C
LABORATORY BENCH SHEETS
Shenandoah Universityrsquos Environmental Studies Department developed the Benthic Macroinvertebrate Laboratory Bench Sheet and VSCI Worksheet to record BMI specimen identifications and their VSCI classifications for each sample
This form is based in part on bench sheets developed by the EPA and Virginia DEQ for recording specimen identifications Additional columns on Shenandoah Universityrsquos forms for relative numbers and VSCI classifications make the calculation and verification of VSCI scores easier
Note the column for taxonomistrsquos initials Specimens were identified by students in Shenandoah Universityrsquos research group and then verified or where necessary changed by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality This external review helps assure the validity of the study Originals of these bench sheets with handwritten taxonomistsrsquo initials will be kept on file at Shenandoah Universityrsquos Environmental Studies Department for five years after the completion of this study
r
s
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-1 STATION NAME Mouth LAT N 39deg 12749rsquo (upstream) LONG W 078deg 04209rsquo (upstream) LAT N 39deg 12729rsquo (downstream) LONG W 078deg 04148rsquo (downstream) FORM COMPILED BY Jeremy Tovar W S Bousquet RIVER BASIN Potomac River INVESTIGATORS Marie Dahl Brandon Millholand J W Pangle Sean Robertson Jeremy Tovar Woodward Bousquet Ben Bousquet
Collection date 21 May 2008 Sheet completion date 12 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Hirudinea 3 33 JT BVW PR 8 SP Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Coenagrionidae 2 22 BVW PR 9 CR Ephemeroptera (mayflies) Caenidae 1 11 JT BVW CG 6 SP Ephemerllidae 4 44 JT BVW CG 2 CR Heptageniidae 5 55 JT BVW SC 3 CG Isonychiidae 1 11 BVW CF 3 CG Plecoptera (stoneflies) Perlidae 14 154 JT BVW PR 2 CR Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Corydalidae 1 11 JT BVW PR 5 CR Coleoptera (beetles) Elmidae 24 264 I JT BVW SC 4 CG Dytiscidae 4 44 JT BVW PR 6 GN Psephenidae 3 33 JT BVW SC 4 CG Diptera (true flies) Tipulidae 3 33 JT BVW SH 4 BU Chironomidae 26 286 JT BVW CG 6 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
t
Other Total Individuals 91 1001 Total Taxa 13
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
u
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-1 STATION NAME Mouth LAT N 39deg 12749rsquo (upstream) LONG W 078deg 04209rsquo (upstream) LAT N 39deg 12729rsquo (downstream) LONG W 078deg 04148rsquo (downstream) FORM COMPILED BY Jeremy Tovar W S Bousquet RIVER BASIN Potomac River INVESTIGATORS Brandon Millholand JW Pangle Jeremy Tovar Heather Kepple
Collection date 10 October 2008 Sheet completion date 12 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Oligochaeta 3 35 JT BVW CG 8 BU Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Coenagrionidae 2 23 JT BVW PR 9 CR Ephemeroptera (mayflies) Heptageniidae 12 140 JT BVW SC 3 CG Isonychiidae 2 23 BVW CF 3 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Hydropsychidae 16 186 JT BVW CF 6 CG Philopotamidae 31 360 JT BVW CF 2 CG Hemiptera (true bugs) (Veliidae) (5) - JT BVW ( ) Megaloptera (dobsonflies etc) Corydalidae 10 116 JT BVW PR 5 CR Coleoptera (beetles) Dytiscidae 1 12 JT BVW PR 6 GN Psephenidae 7 81 JT BVW SC 4 CG Diptera (true flies) Tipulidae 2 23 BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
v
Other Total Individuals 86
(91) 999
Total Taxa 10 (11)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009 Veliidae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
w
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-2 STATION NAME Package Plant LAT N 39deg 12958rsquo (upstream) LONG W 078deg 04900 (upstream) LAT N 39deg 12958rsquo (downstream) LONG W 078deg 04822 (downstream) FORM COMPILED BY JW Pangle W Bousquet RIVER BASIN Potomac River INVESTIGATORS JW Pangle S Robertson J Tovar M Dahl B Millholland W Bousquet
Collection date 29 May 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Plecoptera (stoneflies) Perlidae 11 136 I JP BVW PR 1 CR Trichoptera (caddisflies) Hydropsychidae 10 123 I JP BVW CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 14 173 I JP BVW SC 4 CG Diptera (true flies) Chironomidae 32 395 I JP BVW CG 6 BU Simuliidae 14 173 I JP BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
x
Other Total Individuals 81 1000 Total Taxa 5
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-2 STATION NAME Package Plant LAT N 39deg 12958rsquo (upstream) LONG W 078deg 04900 (upstream) LAT N 39deg 12958rsquo (downstream) LONG W 078deg 04822 (downstream) FORM COMPILED BY JW Pangle W Bousquet RIVER BASIN Potomac River INVESTIGATORS JW Pangle Brandon Millholland Jeremy Tovar Heather Kepple
Collection date 10 October 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Heptageniidae 40 392 I WBJP SC 3 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Hydropsychidae 7 69 I JP WB BVW CF 6 CG Philopotamidae 28 275 I JP WB BVW CF 2 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Corydalidae 6 59 I JP WB BVW PR 5 CR Coleoptera (beetles) Psephenidae 7 69 I JP WB BVW SC 4 CG Elmidae 8 78 I JP WB BVW SC 4 CG Diptera (true flies) Tipulidae 6 59 I JP WB BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
y
z
Other Total Individuals 102 1001 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-3 STATION NAME Rt 664 Bridge LAT N 39deg 12934rsquo (upstream) LONG W 078deg 05019rsquo (upstream) LAT N 39deg 12951rsquo (downstream) LONG W 078deg 04958rsquo (downstream) FORM COMPILED BY S Robertson W Bousquet RIVER BASIN Potomac River INVESTIGATORS SRobertson BMillholland WBousquet MDahl JPangle
Collection date 28 May 2008 Sheet completion date 11 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Leptophlebiidae 1 15 I SGR BVW CG 2 CR Baetidae 1 15 I BVW CG 5 CG Plecoptera (stoneflies) Perlidae 3 46 I SGR BVW PR 1 CR Nemouridae 1 15 I BVW SH 3 CR Trichoptera (caddisflies) Hydropsychidae 5 77 I SGR BVW CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 27 415 I SGR BVW SC 4 CG Psephenidae 2 31 A SGR BVW SC 4 CG Diptera (true flies) Simuliidae 5 77 I SGR BVW CF 6 CG Chironomidae 20 308 I SGR BVW CG 6 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
aa
bb
Other Total Individuals 65 999 Total Taxa 9
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-3 STATION NAME Rt 664 Bridge LAT N 39deg 12934rsquo (upstream) LONG W 078deg 05019rsquo (upstream) LAT N 39deg 12951rsquo (downstream) LONG W 078deg 04958rsquo (downstream) FORM COMPILED BY S Robertson RIVER BASIN Potomac River INVESTIGATORS S Robertson M Dahl A Fisher H Kepple
Collection date 10 Oct 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Heptageniidae 2 20 I SGR SC 3 CG Isonychiidae 2 20 I BVW CF 2 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Philopotamidae 36 367 I SGR CF 2 CG Hydropsychidae 47 480 I SGR CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 2 20 A SGR SC 4 CG Psephenidae 8 82 A SGR SC 4 CG Diptera (true flies) Tabanidae 1 10 I SGR PR 7 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
cc
dd
Other Total Individuals 98 999 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-4 STATION NAME Unnamed Tributary LAT (upstream) N 39deg 13035rsquo LONG (upstream) W 078deg 05129rsquo LAT (downstream) N 39deg 13000rsquo LONG (downstream) W 078deg 5086rsquo FORM COMPILED BY A Fisher M Dahl rev W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson A Fisher H Kepple
Collection date 31 May 2008 Sheet completion date rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Flatworms (Turbellaria) Worms (Oligochaeta) Naididae 1 14 - BVW CG 8 BU Leeches (Hirudinea) Snails (Gastropoda) Clams (Bivalvia) Isopods (Isopoda) Asellidae 3 42 A AF BVW CG 8 SP Scuds (Amphipoda) Crayfish Shrimp (Decapoda) Water Mites (Hydracarina) Dragonflies (Odonata Anisoptera) Damselflies (Odonata Zygoptera) Mayflies (Ephemeroptera) Stoneflies (Plecoptera) Perlidae 2 28 I AF BVW PR 1 CR Caddisflies (Trichoptera) True Bugs (Hemiptera) Corixidae 1 14 I AF BVW MP 9 DV Dobsonflies (Megaloptera) Beetles (Coleoptera) Dytiscidae 1 14 I AF BVW PR 6 GN True Flies (Diptera) Chironomidae 61 847 I AF BVW CG 6 BU Simuliidae 3 42 I AF BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
ee
ff
Other Total Individuals 72 1001 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-4 STATION NAME Unnamed Tributary LAT (upstream) N 39deg 13035rsquo LONG (upstream) W 078deg 05129rsquo LAT (downstream) N 39deg 13000rsquo LONG (downstream) W 078deg 5086rsquo FORM COMPILED BY Andrew G M Fisher W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson A Fisher H Kepple
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Flatworms (Turbellaria) Worms (Oligochaeta) Lumbriculidae 2 32 A AF BVW CG 8 BU Leeches (Hirudinea) Snails (Gastropoda) Lymnaeidae 1 16 A BVW CG 9 GN Clams (Bivalvia) Isopods (Isopoda) Scuds (Amphipoda) Crayfish Shrimp (Decapoda) Cambaridae 1 16 A AF BVW GN 5 GN Water Mites (Hydracarina) Dragonflies (Odonata Anisoptera) Damselflies (Odonata Zygoptera) Calopterygidae 4 63 I BVW PR 7 CL Mayflies (Ephemeroptera) Tricorythidae 1 16 I BVW CG 5 SP Stoneflies (Plecoptera) Caddisflies (Trichoptera) Phryganeidae 4 63 I AF BVW SH 4 CR Hydropsychidae 6 95 I AF BVW CF 6 CG I True Bugs (Hemiptera) Corixidae 8 127 I AF BVW MP 9 DV (Veliidae ) (7) - I AF BVW ( ) Dobsonflies (Megaloptera) Sialidae 3 48 I AF BVW PR 7 SP Corydalidae 2 32 I AF BVW PR 5 CR Beetles (Coleoptera) Haliplidae 1 16 A AF BVW MP 8 GN Dysticidae 7 111 I AF BVW PR 6 GN Hydrophilidae 1 16 A AF BVW PR 8 GN Elmidae 3 48 I BVW SC 4 CG True Flies (Diptera) Chironomidae 8 127 I AF BVW CG 6 BU Simuliidae 7 111 I AF BVW CF 6 CG Tipulidae 4 63 I AF BVW SH 4 GU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
gg
hh
Other Total Individuals 63
(70) 1000
Total Taxa 17 (18)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Veliidae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-5 STATION NAME Lick Run LAT N 39deg 12752rsquo (upstream) LONG W 078deg 05685rsquo (upstream) LAT N 39deg 12784rsquo (downstream) LONG W 078deg 05631rsquo (downstream) FORM COMPILED BY Brandon Millholland W Bousquet RIVER BASIN Potomac River INVESTIGATORS J Pangle S Robertson W Bousquet JTovar M Dahl
Collection date 29 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Gomphidae 1 15 I BVW PR 3 BU Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Leptophlebiidae 11 167 A LBM BVW CG 2 CR Plecoptera (stoneflies) Perlidae 10 152 A LBM BVW PR 1 CR Perlodiae 1 15 A LBM BVW PR 2 CR Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 5 76 A LBM BVW SC 4 CG Elmidae 2 30 I LBM BVW SC 4 CG Diptera (true flies) (Culicidae) (3) - P LMB BVW ( ) Chironomidae 23 348 I LBM BVW CG 6 BU Simuliidae 13 197 I LBM BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
ii
jj
Other Total Individuals 66
(69) 1000
Total Taxa 7 (8)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Culicidae (Diptera) pupae not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl J Pangle S Robertson W Bousquet B Millholland J Tovar H Kepple
Collection date 30 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 1 12 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Asellidae 114 965 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Plecoptera (stoneflies) Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 2 23 A MRD BVW SC 4 CG Diptera (true flies)
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
kk
ll
Other Total Individuals 117 1000 Total Taxa 3
Notes Subsample taken 29 October 2009 All taxonomic determinations by MRD were confirmed by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson K McCann A Fisher H Kepple A Barrett
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 2 17 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Sphaeriidae 2 17 I MRD BVW CF 8 BU Isopoda (isopods) Asellidae 99 853 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Calopterygidae 2 17 A MRD BVW PR 7 CL Ephemeroptera (mayflies) Leptophlebiidae 2 17 I MRD BVW CG 2 CR Heptageniidae 4 34 A MRD BVW SC 3 CG Plecoptera (stoneflies) Perlidae 1 09 A MRD BVW PR 1 CR Trichoptera (caddisflies) Hemiptera (true bugs) (Gerridae) (1) - A MRD BVW ( ) Corixidae 1 09 A MRD BVW PR 5 CR Megaloptera (dobsonflies etc) Coleoptera (beetles) Dryopidae 1 09 A MRD BVW SC 5 CG Diptera (true flies) Tipulidae 2 17 I BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
mm
nn
Other Total Individuals 116
(117) 999
Total Taxa 10 (11)
Notes Subsample taken 6 November 2008 Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009 Gerridae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
- Disclaimer ii
- Title Page iii
- Acknowledgements v
- Table of Contents vii
- SECTION 2 RESULTS DATA TABLES 7
- OVERVIEW AND FINDINGS
- Introduction
- RESULTS DATA TABLES
- REFERENCES
- FIELD DATA SHEETS
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- SubstrateType
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- WATER QUALITY ANALYSIS
- THROUGH THE VIRGINIA
- STREAM CONDITION INDEX (VSCI)
- LABORATORY BENCH SHEETS
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
(BMPs) to improve water quality These practices include fencing cattle out of the stream and spring runs and planting more trees in the riparian zone
Methods and Analysis
The research team established a sampling plan and then sampled according to methods the DEQ developed for the VSCI Using a 1 meter x 1 meter seine samples from 2m2 of substrate in riffle zones were to be collected twice at each site once in May 2008 and once in October 2008 At five of the six sites stream characteristics permitted the standard single-habitat sampling procedure At the McCann Farm (Site HLR-6) researchers needed to use multi-habitat sampling See Barbour et al (1999 pp 7-3 ff) for further descriptions of these two methods
All six sites were sampled in May 2008 Low-water conditions in October 2008 prevented the Shenandoah University group from collecting BMIs at Lick Run (HLR-5) that month but the other five sites were sampled successfully As a result the research team obtained eleven samples for analysis in this study Samples were preserved in ethanol for subsequent identification
DEQ procedures require subsampling when the number of BMIs in a given sample exceeds 100 All eleven samples in this study met that criterion Consequently researchers used a Caton tray to produce random subsamples of 90 to 110 specimens from each sample During the analysis that followed some specimens in the subsamples were rejected either because they turned out to be terrestrial species or because they were in the wrong life stage (eg pupae) In most cases the researchers identified specimens to the family level Primary taxonomic references were A Guide to Common Freshwater Invertebrates of North America (Voshell 2002) Fresh-Water Invertebrates of the United States (Pennak 1953) and An Introduction to the Aquatic Insects of North America (Merritt and Cummins 1996) DEQ Regional Aquatic Biologist Billy van Wart reviewed verified and where necessary corrected the identifications
The investigators then used the Virginia Stream Condition Index (VSCI) to produce water quality scores based on the BMIs collected A VSCI score is based on eight different measurement formulas called metrics Each metric is a numeric measure of a biological communityrsquos species composition diversity ecological niches or tolerance to pollution When combined into a single multimetric VSCI score the response of the benthic community to water conditions can be evaluated For further information and worksheets see Appendices B and C respectively
Findings
Table 1 presents the VSCI scores for the eleven samples collected in the Hiatt-Lick Run subwatershed in 2008 Since a sample year consists of two samples the mean scores for each site for the entire year are also included As the table shows
bull None of the 11 samples was rated excellent bull Only 1 of the 11 samples was rated as good bull Only 2 of the 11 samples were rated fair (ie in the ldquogrey zonerdquo between impaired and
unimpaired) bull The remaining 8 samples were in either moderately stressed or severely stressed
condition
3
Site Name and Code Sampling
Date of
TaxaVCSI Score
Water Quality Rating
Mouth (HLR-1) 21 May 2008 13 571 Fair (Gray Zone)
Mouth (HLR-1) 10 Oct 2008 10 608 Fair (Gray Zone)
site average for 2008 590 Fair (Gray Zone)
Package Plant (HLR-2) 29 May 2008 5 370 Severely Stressed
Package Plant (HLR-2) 10 Oct 2008 7 674 Good
site average for 2008 522 Moderately Stressed
Route 664 Bridge (HLR-3) 28 May 2008 9 485 Moderately Stressed
Route 664 Bridge (HLR-3) 10 Oct 2008 7 469 Moderately Stressed
site average for 2008 477 Moderately Stressed
Unnamed Tributary (HLR-4) 31 May 2008 7 173 Severely Stressed
Unnamed Tributary (HLR-4) 10 Oct 2008 17 476 Moderately Stressed
site average for 2008 325 Severely Stressed
Upper Lick Run (HLR-5) 29 May 2008 7 455 Moderately Stressed
site average for 2008 455 Moderately Stressed
McCann Farm (HLR-6) 30 May 2008 3 190 Severely Stressed
McCann Farm (HLR-6) 10 Oct 2008 10 316 Severely Stressed
site average for 2008 253 Severely Stressed
Summary 6 sites 11 samples
36 BMI taxa
VSCI = Virginia Stream Condition Index see Burton and Gerritsen (2003) and Virginia Department of Environmental Quality (2006b and 2008) Table 1 Summary Table of Virginia Stream Condition Index (VSCI) Scores and Water Quality Ratings for Sites in the Hiatt-Lick Run Subwatershed of Opequon Creek Frederick County Virginia 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
4
Improvements in water quality ratings at three sites between May and October suggest that BMPs and other changes may already be having some positive effects on water quality At the Package Plant (Site HLR-2) the VSCI score rose dramatically from 370 (severely stressed) to 674 (good) The small-scale sewage treatment plant as noted above was taken off-line during this period Similarly water quality at the Unnamed Tributary (HLR-4) increased from 173 (severely stressed) to 476 (moderately stressed) Adjacent construction was completed over the summer and grass was planted on the property Management practices on the McCann Farm (HLR-6) may be responsible for improving conditions for aquatic life in Hiatt Runrsquos headwaters Even though the water quality was still rated as severely stressed in October the VSCI score rose from 190 to 316 at this sampling site Further studies are needed to determine whether the changes noted in 2008 persist into the future In 2003 the DEQ began a Total Maximum Daily Load (TMDL) process for Opequon Creek as required by the Water Quality Act of 1987 The TMDL approach establishes a ldquopollution budgetrdquo for each impaired stream for each type of impairment and for each responsible pollutant In conjunction with the DEQ and the Virginia Department of Conservation and Recreation (DCR) Virginia Polytechnic Institutersquos Department of Biosystems Engineering developed a TMDL implementation plan (Opequon Creek IP Steering Committee et al 2006) Monitoring the BMI communities of Opequon Creek and its tributaries is essential to evaluating the progress of the proposed water quality improvement projects
Future Research The relatively small Hiatt-Lick Run subwatershed provides several case studies that can help assess the effectiveness of various water quality management practices not only in Opequon Creek but also in other watersheds in the Chesapeake Bay region and beyond In supporting that goal this study makes two principal contributions 1 It provides baseline data to measure the improvement or degradation of the Hiatt-Lick Run section of the Opequon Creek watershed and 2 It establishes a monitoring framework for the long-term biological monitoring of water quality and the evaluation of best management practices (BMPs) This framework consists of
bull Sampling Sites ndash six stream reaches in the Hiatt-Lick Run subwatershed selected as sampling sites according to EPA and DEQ specifications
bull Data Collection Protocols ndash based on the EPArsquos Rapid Bioassessment Protocols (RBPs)
(Barbour et al 1999) and the DEQrsquos Virginia Stream Index (VSCI) (Burton and Gerritsen 2003 DEQ 2006b and 2008) and
bull Analytical Procedures ndash contained in the Virginia Stream Condition Index (VSCI see
Burton and Gerritsen 2003 DEQ 2006b pp 8 and 33 and DEQ 2008 pp 29-34)
5
6
Section 2
RESULTS DATA TABLES
Site HLR-1 Mouth
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 13 189 6872 EPT taxa 5 117 4273 Ephemeroptera 121 477 2544 Plec +Tric less Hydropsych 154 562 2745 Scrapers 352 467 7536 Chironomidae 286 16 7267 Top 2 Dominant 550 328 6708 Hilsenhoff Biotic Index (HBI) 45 29 775VSCI Score 571
According to the DEQrsquos water quality standards aquatic life at this site is fair (gray zone) See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 2 VSCI Scores for Mouth (HLR-1) Site in Hiatt-Lick Run Sampled on 21 May 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 10 189 5292 EPT taxa 4 117 3423 Ephemeroptera 163 477 3424 Plec +Tric less Hydropsych 360 562 6415 Scrapers 221 467 4736 Chironomidae 00 16 10007 Top 2 Dominant 546 328 6768 Hilsenhoff Biotic Index (HBI) 39 29 859VSCI Score 608
According to the DEQrsquos water quality standards aquatic life at this site is fair (gray zone) See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 3 VSCI Scores for Mouth (HLR-1) Site in Hiatt-Lick Run Sampled 10 October 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
7
Site HLR-2 Package Plant
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 5 189 2642 EPT taxa 2 117 1713 Ephemeroptera 00 477 004 Plec +Tric less Hydropsych 110 562 1965 Scrapers 173 467 3706 Chironomidae 395 16 6157 Top 2 Dominant 568 328 6438 Hilsenhoff Biotic Index (HBI) 50 29 704VSCI Score 370
According to the DEQrsquos water quality standards aquatic life at this site is severely stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 4 VSCI Scores for Package Plant (HLR-2) Site in Hiatt-Lick Run Sampled 29 May 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 7 189 3702 EPT taxa 34 117 2563 Ephemeroptera 392 477 8214 Plec +Tric less Hydropsych 275 562 4895 Scrapers 539 467 10006 Chironomidae 00 16 10007 Top 2 Dominant 667 328 4968 Hilsenhoff Biotic Index (HBI) 32 29 958VSCI Score 674
According to the DEQrsquos water quality standards aquatic life at this site is good See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 5 VSCI Scores for Package Plant (HLR-2) Site in Hiatt-Lick Run Sampled 10 October 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
8
Site HLR-3 Route 664 Bridge
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 9 189 4762 EPT taxa 5 117 4273 Ephemeroptera 30 477 634 Plec +Tric less Hydropsych 61 562 1095 Scrapers 446 467 9556 Chironomidae 308 16 7037 Top 2 Dominant 723 328 4128 Hilsenhoff Biotic Index (HBI) 48 29 732VSCI Score 485
According to the DEQrsquos water quality standards aquatic life at this site is moderately stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 6 VSCI Scores for Route 664 Bridge (HLR-3) Site in Hiatt-Lick Run Sampled 28 May 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 7 189 3702 EPT taxa 4 117 3423 Ephemeroptera 40 477 844 Plec +Tric less Hydropsych 367 562 6535 Scrapers 122 467 2616 Chironomidae 00 16 10007 Top 2 Dominant 847 328 2288 Hilsenhoff Biotic Index (HBI) 42 29 817VSCI Score 469
According to the DEQrsquos water quality standards aquatic life at this site is moderately stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 7 VSCI Scores for Route 664 Bridge (HLR-3) Site in Hiatt-Lick Run Sampled 10 October 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
9
Site HLR-4 Unnamed Tributary
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 7 189 3702 EPT taxa 1 117 853 Ephemeroptera 00 477 004 Plec +Tric less Hydropsych 28 562 495 Scrapers 00 467 006 Chironomidae 847 16 1557 Top 2 Dominant 889 328 1658 Hilsenhoff Biotic Index (HBI) 60 29 563VSCI Score 173
According to the DEQrsquos water quality standards aquatic life at this site is severely stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 8 VSCI Scores for Unnamed Tributary (HLR-4) Site in Hiatt-Lick Run Sampled 31 May 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 17 189 8992 EPT taxa 3 117 2563 Ephemeroptera 16 477 344 Plec +Tric less Hydropsych 63 562 1125 Scrapers 48 467 1036 Chironomidae 127 16 8877 Top 2 Dominant 254 328 10008 Hilsenhoff Biotic Index (HBI) 63 29 521VSCI Score 476
According to the DEQrsquos water quality standards aquatic life at this site is moderately stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 9 VSCI Scores for Unnamed Tributary (HLR-4) Site in Hiatt-Lick Run Sampled 10 October 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
10
Site HLR-5 Lick Run
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 7 189 3702 EPT taxa 3 117 2563 Ephemeroptera 167 477 3504 Plec +Tric less Hydropsych 167 562 2975 Scrapers 106 467 2276 Chironomidae 348 16 6637 Top 2 Dominant 545 328 6778 Hilsenhoff Biotic Index (HBI) 43 29 803VSCI Score 455
According to the DEQrsquos water quality standards aquatic life at this site is moderately stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 10 VSCI Scores for Lick Run (HLR-5) Site in Hiatt-Lick Run Sampled 29 May 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
Note
Water conditions at the Lick Run (HLR-5) site were too low in October 2008 to permit a sample to be taken
11
Site HLR-6 McCann Farm
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 3 189 1592 EPT taxa 0 117 003 Ephemeroptera 00 477 004 Plec +Tric less Hydropsych 00 562 005 Scrapers 23 467 496 Chironomidae 00 16 10007 Top 2 Dominant 988 328 188 Hilsenhoff Biotic Index (HBI) 79 29 296VSCI Score 190
According to the DEQrsquos water quality standards aquatic life at this site is severely stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 11 VSCI Scores for McCann Farm (HLR-6) Site in Hiatt-Lick Run Sampled 30 May 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 10 189 5292 EPT taxa 3 117 2563 Ephemeroptera 51 477 1074 Plec +Tric less Hydropsych 09 562 165 Scrapers 43 467 926 Chironomidae 00 16 10007 Top 2 Dominant 887 328 1688 Hilsenhoff Biotic Index (HBI) 75 29 352VSCI Score 315
According to the DEQrsquos water quality standards aquatic life at this site is severely stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 12 VSCI Scores for McCann Farm (HLR-6) Site in Hiatt-Lick Run Sampled 10 October 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
12
REFERENCES
Barbour Michael T Jeroen Gerritsen Blaine D Snyder and James B Stribling 1999 Rapid bioassessment protocols for use in streams and wadeable rivers periphyton benthic macroinvertebrates and fish Second edition EPA 841-B-99-002 Washington DC US Environmental Protection Agency Office of Water Available online at httpwwwepagovOWOWmonitoringbioasspshtml Burton June and Jeroen Gerritsen 2003 A stream condition index for Virginia non-coastal streams Prepared for the US EPA Office of Science and Technology US EPA Region 3 Environmental Services Division and Virginia Department of Environmental Quality Owings Mills Maryland Tetra Tech Inc Available online at httpwwwdeqvirginiagovwatermonitoringpdf vastrmconpdf Hessler Rachel A Andrea R Martin Heidi D Ruggeri William B Seymour Jr and Woodward S Bousquet 2006 Water quality assessment using benthic macroinvertebrates A study and compilation Winchester Virginia Environmental Studies Program Shenandoah University Occasional publication no 10 Igoe Robert 2006 DEQ identifies nine impaired streams in Clarke Frederick The Winchester Star 15 July B1 Jackson Charlie 2005 Survey targets Opequon cleanup The Winchester Star 19 August B1 Johnston Elizabeth A and Woodward S Bousquet 2003 What happens when it rains A study of water quality in Abrams Creek and Town Run Winchester and Frederick County Virginia Winchester Virginia Environmental Studies Program Shenandoah University Occasional publication no7 Merritt Richard W and Kenneth W Cummins 1996 An introduction to the aquatic insects of North America Dubuque Iowa KendallHunt Publishing Company Opequon Creek IP Steering Committee Virginia Tech Department of Biological Systems Engineering and Center for TMDL and Watershed Studies University of Virginia Institute for Environmental Negotiation and West Virginia University Division of Resource Management 2006 Opequon Creek watershed TMDL implementation plan Blacksburg Virginia Virginia Tech Department of Biological Systems Engineering Available online at httpwwwtmdlbsevteduoutreachC82 Pennak Robert W 1953 Fresh-water invertebrates of the United States New York New York The Ronald Press Company Rosenburg David M and Vincent H Resh 1993 Freshwater biomonitoring and benthic macroinvertebrates New York New York Chapman amp Hall Shenandoah University Public Relations Office 2002 Data collected by SU and its partners helps identify impaired water Winchester Virginia Available online at httpwwwsuedutemp_ newscfmurlnum=32 Thorp James H and Alan P Covich 1991 Ecology and classification of North American freshwater invertebrates San Diego California Academic Press Inc
13
US Environmental Protection Agency 1997 Volunteer stream monitoring a methods manual EPA 841-B-97-003 Washington DC US Environmental Protection Agency Office of Water Van Meter Val 2003 Opequon Creek pollution limits to be established The Winchester Star 4 April B1 Virginia Department of Environmental Quality 2006a Virginia 305(b)303(d) water quality integrated report to Congress and the EPA Administrator for the period January 1 2000 to December 31 2004 Richmond Virginia Virginia Department of Environmental Quality Available online at httpwwwdeqvirginiagovwqair2006html __________ 2006b Using probabilistic data to validate the non-coastal Virginia Stream Condition Index (draft) VDEQ technical bulletin WQA2006-001 Virginia Department of Environmental Quality Available online at httpwwwloudounwatershedorgstream_assessmentreference_documents VA_DEQ_SCI_Validationpdf __________ 2008 Biological monitoring program quality assurance project plan for wadeable streams and rivers Richmond Virginia Virginia Department of Environmental Quality Update available online at httpwwwdeqstatevausbiomonitoringdocumentsBioMonQAPP_13Aug2008pdf Virginia Tech Aquatic Entomology Laboratory 2005 Taxa dictionary [Excel file] Blacksburg Virginia Virginia Tech Department of Biological Systems Engineering 2003a Opequon watershed TMDLs for benthic impairments Abrams Creek and Lower Opequon Creek Frederick and Clarke Counties Virginia Prepared for the Virginia Department of Environmental Quality and Virginia Department of Conservation and Recreation Blacksburg Virginia Available online at httpwwwdeqstate vaustmdlapptmdlsshenrvrabropebcpdf Voshell J Reese Jr 2002 A guide to common freshwater invertebrates of North America Blacksburg Virginia The McDonald amp Woodward Publishing Company
14
APPENDICES
15
16
a
Appendix A
FIELD DATA SHEETS
This appendix presents a Site Characterization Field Data Sheet for each of the six sampling reaches established on Hiatt Run and Lick Run for this study They were filled out during the sampling runs made in May 2008
These forms were adapted by Shenandoah University from forms used by the EPA and Virginia DEQ for recording characteristics of sampling sites
b
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Mouth STATION _HLR-1_____ STREAM CLASS LAT N 39 12749 (upstream) LONG W 078 04209(upstream) LAT N 39 12729 (downstream) LONG W 078 04148 (downstream) FORM COMPILED BY Jeremy Tovar RIVER BASIN Potomac River INVESTIGATORS JPangle B Millholland MDahl SRobertson W Bousquet JTovar BBousquet
DATE May 21 2008_________ TIME_250 PM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature __24___degC showers (intermittent) Comments _______________________
___ cloud cover _0_ ______________________________
X clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x ( Significant springs andor seeps) x (Valley ) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS
c
d
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence x FieldPasture Industrial Obvious sources Agricultural Other ____________ Stream scour steep slope erosion x Residential Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left Field and young thin deciduous forest 100 Bank=Grass cobble and shrub Right 90 maturing deciduous forest 45 shale slope Bank=Bedrock soil and herbs Indicate the dominant vegetation type(s) and record the dominant species
x Trees Shrubs x (Grasses) Forbs Dominant species Oak species and mix deciduous
INSTREAM FEATURES
Measured reach length ___100__m Canopy Cover Estimated stream width _35__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area __350_m2
Estimated stream depth _008_m Proportion of Reach Represented by Stream High water mark _15_m Morphology Types Surface velocity (at thalweg) _05__msec x Riffle__75___ x Run__20___ x Pool__5___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___00__ m2 Density of LWD _-_ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __15_____ x Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present Algae
WATER QUALITY Temperature __19___degC Water Odors x NormalNone Sewage Specific Conductance - Petroleum Chemical Fishy Other___________________ Dissolved Oxygen - Water Surface Oils Slick Sheen Globs Flecks pH __-_ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used _____NA________ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic x None Relict shells Other______None_____ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 8 Detritus sticks wood coarse plant materials
0 Boulder gt256 mm (10rsquorsquo) 0
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 51 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 40 Sand 006-2 mm (gritty) 1 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University
SITE CHARACTERIZATION FIELD DATA SHEET FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Package Plant STATION HLR-2 STREAM CLASS LAT N 39 129580 (upstream) LONG W 078 04900 (upstream) LAT N 39 129581 (downstream) LONG W 078 04822 (downstream) FORM COMPILED BY JW Pangle RIVER BASIN Potomac River INVESTIGATORS JPangle JTovar MDahl B Millholland SRobertson W Bousquet
DATE__19 May 2008_ TIME__1240__ PM
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature _____degC showers (intermittent) Comments __Sunny Day_____________
_5__ cloud cover _0__ ______________________________
x clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x (Significant springs andor seeps) x (Valley) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS
e
f
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence FieldPasture Industrial Obvious sources Agricultural Other ____________ __Upstream Construction and Stream bank Scour Residential __________________________________ Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left Deciduous forest with thick herb and shrub layers bank= vegetation and rocks Right Deciduous forest with thick herb and shrub layers bank=vegetation soil and rocks Indicate the dominant vegetation type(s) and record the dominant species
x Trees x Shrubs Grasses Forbs Dominant species Black Walnut White Ash Hackberry and American Sycamore
INSTREAM FEATURES
Measured reach length 100 m Canopy Cover Estimated stream width _35__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area __350_m2
Estimated stream depth __15__m Proportion of Reach Represented by Stream High water mark __22___m Morphology Types Surface velocity (at thalweg) _035__msec x Riffle__30___ x Run__30___ x Pool__40___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___10____ m2 Density of LWD _______ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __30___ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present__________________________________________________________
WATER QUALITY Temperature __18___degC Water Odors x NormalNone Sewage Specific Conductance __-____ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ____-____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used _____NA____ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other___None_____ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach SubstrateType Characteristic
Composition in Sampling Reach
Bedrock 3 Detritus sticks wood coarse plant materials
2 Boulder gt256 mm (10rsquorsquo) 5
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 70 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 22 Sand 006-2 mm (gritty) 0 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiattrsquos Run LOCATION Rt 664 Bridge STATION HLR-3 STREAM CLASS LAT N 39 12934 (upstream) LONG W 078 05019 (upstream) LAT N 39 12958 (downstream) LONG W 078 04958 (downstream) FORM COMPILED BY Sean Robertson RIVER BASIN Potomac River INVESTIGATORS SRobertson BMillholland WBousquet MDahl JPangle
DATE 28 May 2008 TIME 1127 AM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality 2008
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature __14___degC showers (intermittent) x Trace Comments _______________________
_65 x cloud cover ___ ______________________________
x clearsunny __________________________________
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x (Significant springs andor seeps) x (Valley) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS
g
h
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence Some evidence x FieldPasture Industrial x Obvious sources Agricultural x Other Old Package Plant Downstream x Residential Bridge and Road Power line and Pump station Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left 70 field 30 shrubs and deciduous trees Bank=Soil and vegetation Right Maturing deciduous forest steep slope Bank=Shale Bedrock Indicate the dominant vegetation type(s) and record the dominant species
x Trees x Shrubs x Grasses Forbs Dominant species Sycamore and Red Oaks
INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width __51___m Fully open x Partly open Partly shaded Fully shaded Sampling reach area _510_m2
Estimated stream depth __135___m Proportion of Reach Represented by Stream High water mark __-___m Morphology Types Surface velocity (at thalweg) __20___msec x Riffle_40_ x Run_50_ x Pool 10 Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___0____ m2 Density of LWD ___-____ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation ___50____ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present Brown Algae
WATER QUALITY Temperature __17___degC Water Odors x NormalNone Sewage Specific Conductance ___-___ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ___-_____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used ____NA_______ Clear x Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other____None____ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 15 Detritus sticks wood coarse plant materials
0 Boulder gt256 mm (10rsquorsquo) 15
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 62 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 8 Sand 006-2 mm (gritty) 0 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Unnamed Tributary STATION HLR-4 STREAM CLASS LAT N 39 13035 (upstream) LONG W 078 05129 (upstream) LAT N 39 13000 (downstream) LONG W 078 05086 (downstream) FORM COMPILED BY Marie Dahl RIVER BASIN Potomac River INVESTIGATORS M Dahl HKepple SRobertson AFisher
DATE 31 May 2008 TIME 5 PM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality 2008
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) YES x NO
rain (steady rain) Air Temperature __22___degC showers (intermittent) Comments _______________________
_0__ cloud cover _0__ ______________________________
x clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area __-___ km2 mi2 Montane Significant springs andor seeps x Valley Marsh or swamp Montane amp Valley Mixture of origins Other ___________________
COMMENTS Snowden Bridge development (upstream) and sewer line
i
j
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence x FieldPasture Industrial Obvious sources Agricultural Other ____________ Construction upstream and adjacent x Residential Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left Forested with rocky banks field across silt fence and 2m tree strip Right Forested with some rocky banks and forest through the highway Indicate the dominant vegetation type(s) and record the dominant species
x Trees x Shrubs x Grasses Forbs Dominant species White Ash and Hackberry
INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width ___38__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area __380__m2
Estimated stream depth __12__cm Proportion of Reach Represented by Stream High water mark __2___m Morphology Types Surface velocity (at thalweg) __46___msec x Riffle__25___ x Run__70___ x Pool__5___ Channelized Yes x No Dam Present x Yes No
LARGE WOODY DEBRIS
LWD _______ m2 Density of LWD _______ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation ___NA____ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present___Algae___
WATER QUALITY Temperature __16___degC Water Odors x NormalNone Sewage Specific Conductance __-____ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ___-_____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used ____NA_____ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells x Other___Shale_______ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 0 Detritus sticks wood coarse plant materials
10 Boulder gt256 mm (10rsquorsquo) 5
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 45 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 30 Sand 006-2 mm (gritty) 0 Marl grey shell
fragments 0
Silt 0004-006 mm 20 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Lick Run STATION HLR-5 STREAM CLASS LAT N 39 127517 (upstream) LONG W 078 05685(upstream) LAT N 39 12784 (downstream) LONG W 078 05631 (downstream) FORM COMPILED BY B Millholland RIVER BASIN Potomac River INVESTIGATORS B Millholland JTovar SRobertson MDahl WBousquet JPangle
DATE 29 May 2008 TIME 1040 AM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project
k
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature __15___degC showers (intermittent) Comments _______________________
_5_ X cloud cover _0__ ______________________________
x clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane Significant springs andor seeps x Valley Marsh or swamp Montane amp Valley Mixture of origins Other ___________________
COMMENTS Lick Run appears to be intermittent and may be dry in Fall 2008
l
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence x FieldPasture Industrial Obvious sources Agricultural Other ____________ Adjacent logging stream bank scour Residential Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left 85 young deciduous forest 15 logged Bank=Soil vegetation and cobbles Right Young deciduous forest on 30 slope Bank= Soil vegetation and cobble Indicate the dominant vegetation type(s) and record the dominant species
x Trees Shrubs Grasses Forbs Dominant species Mixed Deciduous INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width __11__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area 110 m2
Estimated stream depth _009__m Proportion of Reach Represented by Stream High water mark __96___m Morphology Types Surface velocity (at thalweg) __01___msec x Riffle__30___ x Run__5___ x Pool__65___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___0____ m2 Density of LWD ___-____ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __70___ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present__________________________________________________________
WATER QUALITY Temperature __14___degC Water Odors x NormalNone Sewage Specific Conductance __-__ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ___-___ Water Surface Oils Slick Sheen Globs Flecks pH __-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used _____NA______ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other_____None_____
SEDIMENT AND SUBSTRATE
Odors Deposits Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other________________ x Other Slightly Salty Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 0 Detritus sticks wood coarse plant materials
5 Boulder gt256 mm (10rsquorsquo) 0
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 75 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 23 Sand 006-2 mm (gritty) 2 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION McCann Farm STATION HLR-6 STREAM CLASS LAT N 39 13076 (upstream) LONG W 078 06539 (upstream) LAT N 39 13063 (downstream) LONG W 078 06473 (downstream) FORM COMPILED BY MDahl RIVER BASIN Potomac River INVESTIGATORS MDahl JPangle SRobertson W Bousqet BMillholland JTovar H Kepple
DATE 30 May 2008 TIME 1050 AM (start)
PROJECT NAME
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) YES x NO
rain (steady rain) Air Temperature __18___degC showers (intermittent) Comments _______________________
_85_x cloud cover _10__ ______________________________
clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x (Significant springs andor seeps) x ( Valley) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS Multi-habitat sampling with D-frame nets and seine
m
n
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial x No evidence Some evidence x FieldPasture Industrial Obvious sources x Agricultural Other ____________ Minor stream bank scour Residential Local Watershed Erosion x None Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left 35 bank grasses scattered trees and some bushes Right High bank with grasses and scattered trees Indicate the dominant vegetation type(s) and record the dominant species
x Trees Shrubs Grasses Forbs Dominant species Black Walnut and Festuca elatior
INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width __2___m Fully open Partly open x Partly shaded Fully shaded Sampling reach area __200_m2
Estimated stream depth __03_m Proportion of Reach Represented by Stream High water mark __1___m Morphology Types Surface velocity (at thalweg) __166_msec x Riffle__0___ x Run__55___ x Pool__45___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___2____ m2 Density of LWD ___-____ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __20_____ x Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present ____Glyceria (grass)sp__________
WATER QUALITY Temperature __16___degC Water Odors x NormalNone Sewage Specific Conductance ___-___ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ____-____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used ______NA___ Clear x Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other__NONE__ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 0 Detritus sticks wood coarse plant materials
5 Boulder gt256 mm (10rsquorsquo) 0
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 27 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 38 Sand 006-2 mm (gritty) 5 Marl grey shell
fragments 0 (lt1)
Silt 0004-006 mm 25 Clay lt 0004 mm (slick) 5 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Appendix B
WATER QUALITY ANALYSIS THROUGH THE VIRGINIA
STREAM CONDITION INDEX (VSCI)
The Virginia Stream Condition Index (VSCI) relies on eight metrics to categorize stream health According to Burton and Gerritsen (2003 p 1-1) this index ldquohellip can be used as a primary indicator of ecosystem health and can identify impairment with respect to the reference (or natural) conditionrdquo These metrics are numeric measures of a biological communityrsquos species composition diversity ecological niches or tolerance to pollution
Table 13 on the next page describes each VSCI metric lists its formula and provides the best standard value (BSV) for that metric Based on a 100-point scale the BSV is the score that reflects optimum conditions for aquatic life ie the best water quality value possible (DEQ 2006b pp 8 33) The table also explains whether the score increases or decreases with environmental stress
As Table 13 indicates each of the eight VSCI metrics is calculated individually Each formula provides a unitless score based on a 100-point scale with 0 as the worst possible score and 100 as the best Any metric that provides a result above 100 is changed to 100 in order for the index to work properly After the eight metrics are calculated they are summed and averaged to provide a final VSCI score on a 100-point scale
Based on statistical analysis of Virginiarsquos streams and their BMI communities the DEQ (2006b p 8) currently uses the following tiers to interpret VSCI scores
VSCI Scores Aquatic Life Use
(ALI) Tiers
lt 42 severely stressed
42-55 moderately stressed
55-63 unknownfair (gray zone)
63-73 good
gt73 excellent
Section 2 of this report presents the results of calculations for the six BMI sampling sites the research team studied in the Hiatt-Lick Run subwatershed in 2008
o
p
1 Total taxa the number of distinct taxa in the sample measures the overall diversity of the macroinvertebrate assemblage The best standard value (BSV) is 189 Score = 100 x (X189) Total taxa decreases with stress 2 EPT taxa the sum of the distinct taxa in the orders Ephemeroptera (mayflies) Plecoptera (stoneflies) and Trichoptera (caddisflies) These orders are known to be sensitive to pollution The best standard value (BSV) is 117 Score = 100 x (X117) EPT taxa decreases with stress 3 Percent Ephemeroptera the percent abundance of individuals in the sample that belong to the order Ephemeroptera (mayflies) The best standard value (BSV) is 477 Score = 100 x (X477) Percent Ephemeroptera decreases with stress 4 Percent Plecoptera + Trichoptera less Hydropsychidae the percent abundance of individuals in the sample that belong to the orders Plecoptera (stoneflies) and Trichoptera (caddisflies) excluding the pollution-tolerant caddisfly family Hydropsychidae The best standard value (BSV) is 562 Score = 100 x (X562) Percent Plecoptera + Trichoptera less Hydropsychidae decreases with stress 5 Percent Scrapers the percent abundance of individuals in the sample whose primary functional mechanism for feeding is grazing on the substrate or periphyton (ie attached algae and associated material) The best standard value (BSV) is 467 Score = 100 x (X467) Percent scrapers decreases with stress 6 Percent Chironomidae the percent abundance of individuals in the sample that are Chironomidae (non-biting midge) larvae and pupae The best standard value (BSV) is 16 Score = 100 x [(100-X)(100-16)] Percent Chironomidae increases with stress 7 Percent Top Two Dominant the percent abundance of the two most frequent taxa present in the sample The best standard value (BSV) is 328 Score = 100 x [(100-X)(100-328)] The Percent Top 2 Dominant increases with stress 8 Hilsenhoff Biotic Index (HBI) an abundance-weighted average pollution tolerance of all the individuals within a sample keyed to the family level Tolerance ratings range from 1 (highly intolerant) to 10 (highly tolerant) The best standard value (BSV) is 29 Score = 100 x [(10-X)(10-29)] The Hilsenhoff Biotic Index (HBI) increases with stress ______________________________________________________________________________ Table 13 Description of Benthic Macroinvertebrate VSCI Metrics Includes responses (increases decreases) of each VSCI metric to degraded water quality conditions Adapted from Burton and Gerritsen (2003 pp 3-15 3-16 6-2) and DEQ (2008)
q
Appendix C
LABORATORY BENCH SHEETS
Shenandoah Universityrsquos Environmental Studies Department developed the Benthic Macroinvertebrate Laboratory Bench Sheet and VSCI Worksheet to record BMI specimen identifications and their VSCI classifications for each sample
This form is based in part on bench sheets developed by the EPA and Virginia DEQ for recording specimen identifications Additional columns on Shenandoah Universityrsquos forms for relative numbers and VSCI classifications make the calculation and verification of VSCI scores easier
Note the column for taxonomistrsquos initials Specimens were identified by students in Shenandoah Universityrsquos research group and then verified or where necessary changed by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality This external review helps assure the validity of the study Originals of these bench sheets with handwritten taxonomistsrsquo initials will be kept on file at Shenandoah Universityrsquos Environmental Studies Department for five years after the completion of this study
r
s
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-1 STATION NAME Mouth LAT N 39deg 12749rsquo (upstream) LONG W 078deg 04209rsquo (upstream) LAT N 39deg 12729rsquo (downstream) LONG W 078deg 04148rsquo (downstream) FORM COMPILED BY Jeremy Tovar W S Bousquet RIVER BASIN Potomac River INVESTIGATORS Marie Dahl Brandon Millholand J W Pangle Sean Robertson Jeremy Tovar Woodward Bousquet Ben Bousquet
Collection date 21 May 2008 Sheet completion date 12 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Hirudinea 3 33 JT BVW PR 8 SP Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Coenagrionidae 2 22 BVW PR 9 CR Ephemeroptera (mayflies) Caenidae 1 11 JT BVW CG 6 SP Ephemerllidae 4 44 JT BVW CG 2 CR Heptageniidae 5 55 JT BVW SC 3 CG Isonychiidae 1 11 BVW CF 3 CG Plecoptera (stoneflies) Perlidae 14 154 JT BVW PR 2 CR Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Corydalidae 1 11 JT BVW PR 5 CR Coleoptera (beetles) Elmidae 24 264 I JT BVW SC 4 CG Dytiscidae 4 44 JT BVW PR 6 GN Psephenidae 3 33 JT BVW SC 4 CG Diptera (true flies) Tipulidae 3 33 JT BVW SH 4 BU Chironomidae 26 286 JT BVW CG 6 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
t
Other Total Individuals 91 1001 Total Taxa 13
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
u
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-1 STATION NAME Mouth LAT N 39deg 12749rsquo (upstream) LONG W 078deg 04209rsquo (upstream) LAT N 39deg 12729rsquo (downstream) LONG W 078deg 04148rsquo (downstream) FORM COMPILED BY Jeremy Tovar W S Bousquet RIVER BASIN Potomac River INVESTIGATORS Brandon Millholand JW Pangle Jeremy Tovar Heather Kepple
Collection date 10 October 2008 Sheet completion date 12 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Oligochaeta 3 35 JT BVW CG 8 BU Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Coenagrionidae 2 23 JT BVW PR 9 CR Ephemeroptera (mayflies) Heptageniidae 12 140 JT BVW SC 3 CG Isonychiidae 2 23 BVW CF 3 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Hydropsychidae 16 186 JT BVW CF 6 CG Philopotamidae 31 360 JT BVW CF 2 CG Hemiptera (true bugs) (Veliidae) (5) - JT BVW ( ) Megaloptera (dobsonflies etc) Corydalidae 10 116 JT BVW PR 5 CR Coleoptera (beetles) Dytiscidae 1 12 JT BVW PR 6 GN Psephenidae 7 81 JT BVW SC 4 CG Diptera (true flies) Tipulidae 2 23 BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
v
Other Total Individuals 86
(91) 999
Total Taxa 10 (11)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009 Veliidae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
w
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-2 STATION NAME Package Plant LAT N 39deg 12958rsquo (upstream) LONG W 078deg 04900 (upstream) LAT N 39deg 12958rsquo (downstream) LONG W 078deg 04822 (downstream) FORM COMPILED BY JW Pangle W Bousquet RIVER BASIN Potomac River INVESTIGATORS JW Pangle S Robertson J Tovar M Dahl B Millholland W Bousquet
Collection date 29 May 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Plecoptera (stoneflies) Perlidae 11 136 I JP BVW PR 1 CR Trichoptera (caddisflies) Hydropsychidae 10 123 I JP BVW CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 14 173 I JP BVW SC 4 CG Diptera (true flies) Chironomidae 32 395 I JP BVW CG 6 BU Simuliidae 14 173 I JP BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
x
Other Total Individuals 81 1000 Total Taxa 5
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-2 STATION NAME Package Plant LAT N 39deg 12958rsquo (upstream) LONG W 078deg 04900 (upstream) LAT N 39deg 12958rsquo (downstream) LONG W 078deg 04822 (downstream) FORM COMPILED BY JW Pangle W Bousquet RIVER BASIN Potomac River INVESTIGATORS JW Pangle Brandon Millholland Jeremy Tovar Heather Kepple
Collection date 10 October 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Heptageniidae 40 392 I WBJP SC 3 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Hydropsychidae 7 69 I JP WB BVW CF 6 CG Philopotamidae 28 275 I JP WB BVW CF 2 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Corydalidae 6 59 I JP WB BVW PR 5 CR Coleoptera (beetles) Psephenidae 7 69 I JP WB BVW SC 4 CG Elmidae 8 78 I JP WB BVW SC 4 CG Diptera (true flies) Tipulidae 6 59 I JP WB BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
y
z
Other Total Individuals 102 1001 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-3 STATION NAME Rt 664 Bridge LAT N 39deg 12934rsquo (upstream) LONG W 078deg 05019rsquo (upstream) LAT N 39deg 12951rsquo (downstream) LONG W 078deg 04958rsquo (downstream) FORM COMPILED BY S Robertson W Bousquet RIVER BASIN Potomac River INVESTIGATORS SRobertson BMillholland WBousquet MDahl JPangle
Collection date 28 May 2008 Sheet completion date 11 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Leptophlebiidae 1 15 I SGR BVW CG 2 CR Baetidae 1 15 I BVW CG 5 CG Plecoptera (stoneflies) Perlidae 3 46 I SGR BVW PR 1 CR Nemouridae 1 15 I BVW SH 3 CR Trichoptera (caddisflies) Hydropsychidae 5 77 I SGR BVW CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 27 415 I SGR BVW SC 4 CG Psephenidae 2 31 A SGR BVW SC 4 CG Diptera (true flies) Simuliidae 5 77 I SGR BVW CF 6 CG Chironomidae 20 308 I SGR BVW CG 6 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
aa
bb
Other Total Individuals 65 999 Total Taxa 9
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-3 STATION NAME Rt 664 Bridge LAT N 39deg 12934rsquo (upstream) LONG W 078deg 05019rsquo (upstream) LAT N 39deg 12951rsquo (downstream) LONG W 078deg 04958rsquo (downstream) FORM COMPILED BY S Robertson RIVER BASIN Potomac River INVESTIGATORS S Robertson M Dahl A Fisher H Kepple
Collection date 10 Oct 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Heptageniidae 2 20 I SGR SC 3 CG Isonychiidae 2 20 I BVW CF 2 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Philopotamidae 36 367 I SGR CF 2 CG Hydropsychidae 47 480 I SGR CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 2 20 A SGR SC 4 CG Psephenidae 8 82 A SGR SC 4 CG Diptera (true flies) Tabanidae 1 10 I SGR PR 7 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
cc
dd
Other Total Individuals 98 999 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-4 STATION NAME Unnamed Tributary LAT (upstream) N 39deg 13035rsquo LONG (upstream) W 078deg 05129rsquo LAT (downstream) N 39deg 13000rsquo LONG (downstream) W 078deg 5086rsquo FORM COMPILED BY A Fisher M Dahl rev W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson A Fisher H Kepple
Collection date 31 May 2008 Sheet completion date rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Flatworms (Turbellaria) Worms (Oligochaeta) Naididae 1 14 - BVW CG 8 BU Leeches (Hirudinea) Snails (Gastropoda) Clams (Bivalvia) Isopods (Isopoda) Asellidae 3 42 A AF BVW CG 8 SP Scuds (Amphipoda) Crayfish Shrimp (Decapoda) Water Mites (Hydracarina) Dragonflies (Odonata Anisoptera) Damselflies (Odonata Zygoptera) Mayflies (Ephemeroptera) Stoneflies (Plecoptera) Perlidae 2 28 I AF BVW PR 1 CR Caddisflies (Trichoptera) True Bugs (Hemiptera) Corixidae 1 14 I AF BVW MP 9 DV Dobsonflies (Megaloptera) Beetles (Coleoptera) Dytiscidae 1 14 I AF BVW PR 6 GN True Flies (Diptera) Chironomidae 61 847 I AF BVW CG 6 BU Simuliidae 3 42 I AF BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
ee
ff
Other Total Individuals 72 1001 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-4 STATION NAME Unnamed Tributary LAT (upstream) N 39deg 13035rsquo LONG (upstream) W 078deg 05129rsquo LAT (downstream) N 39deg 13000rsquo LONG (downstream) W 078deg 5086rsquo FORM COMPILED BY Andrew G M Fisher W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson A Fisher H Kepple
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Flatworms (Turbellaria) Worms (Oligochaeta) Lumbriculidae 2 32 A AF BVW CG 8 BU Leeches (Hirudinea) Snails (Gastropoda) Lymnaeidae 1 16 A BVW CG 9 GN Clams (Bivalvia) Isopods (Isopoda) Scuds (Amphipoda) Crayfish Shrimp (Decapoda) Cambaridae 1 16 A AF BVW GN 5 GN Water Mites (Hydracarina) Dragonflies (Odonata Anisoptera) Damselflies (Odonata Zygoptera) Calopterygidae 4 63 I BVW PR 7 CL Mayflies (Ephemeroptera) Tricorythidae 1 16 I BVW CG 5 SP Stoneflies (Plecoptera) Caddisflies (Trichoptera) Phryganeidae 4 63 I AF BVW SH 4 CR Hydropsychidae 6 95 I AF BVW CF 6 CG I True Bugs (Hemiptera) Corixidae 8 127 I AF BVW MP 9 DV (Veliidae ) (7) - I AF BVW ( ) Dobsonflies (Megaloptera) Sialidae 3 48 I AF BVW PR 7 SP Corydalidae 2 32 I AF BVW PR 5 CR Beetles (Coleoptera) Haliplidae 1 16 A AF BVW MP 8 GN Dysticidae 7 111 I AF BVW PR 6 GN Hydrophilidae 1 16 A AF BVW PR 8 GN Elmidae 3 48 I BVW SC 4 CG True Flies (Diptera) Chironomidae 8 127 I AF BVW CG 6 BU Simuliidae 7 111 I AF BVW CF 6 CG Tipulidae 4 63 I AF BVW SH 4 GU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
gg
hh
Other Total Individuals 63
(70) 1000
Total Taxa 17 (18)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Veliidae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-5 STATION NAME Lick Run LAT N 39deg 12752rsquo (upstream) LONG W 078deg 05685rsquo (upstream) LAT N 39deg 12784rsquo (downstream) LONG W 078deg 05631rsquo (downstream) FORM COMPILED BY Brandon Millholland W Bousquet RIVER BASIN Potomac River INVESTIGATORS J Pangle S Robertson W Bousquet JTovar M Dahl
Collection date 29 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Gomphidae 1 15 I BVW PR 3 BU Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Leptophlebiidae 11 167 A LBM BVW CG 2 CR Plecoptera (stoneflies) Perlidae 10 152 A LBM BVW PR 1 CR Perlodiae 1 15 A LBM BVW PR 2 CR Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 5 76 A LBM BVW SC 4 CG Elmidae 2 30 I LBM BVW SC 4 CG Diptera (true flies) (Culicidae) (3) - P LMB BVW ( ) Chironomidae 23 348 I LBM BVW CG 6 BU Simuliidae 13 197 I LBM BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
ii
jj
Other Total Individuals 66
(69) 1000
Total Taxa 7 (8)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Culicidae (Diptera) pupae not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl J Pangle S Robertson W Bousquet B Millholland J Tovar H Kepple
Collection date 30 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 1 12 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Asellidae 114 965 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Plecoptera (stoneflies) Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 2 23 A MRD BVW SC 4 CG Diptera (true flies)
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
kk
ll
Other Total Individuals 117 1000 Total Taxa 3
Notes Subsample taken 29 October 2009 All taxonomic determinations by MRD were confirmed by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson K McCann A Fisher H Kepple A Barrett
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 2 17 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Sphaeriidae 2 17 I MRD BVW CF 8 BU Isopoda (isopods) Asellidae 99 853 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Calopterygidae 2 17 A MRD BVW PR 7 CL Ephemeroptera (mayflies) Leptophlebiidae 2 17 I MRD BVW CG 2 CR Heptageniidae 4 34 A MRD BVW SC 3 CG Plecoptera (stoneflies) Perlidae 1 09 A MRD BVW PR 1 CR Trichoptera (caddisflies) Hemiptera (true bugs) (Gerridae) (1) - A MRD BVW ( ) Corixidae 1 09 A MRD BVW PR 5 CR Megaloptera (dobsonflies etc) Coleoptera (beetles) Dryopidae 1 09 A MRD BVW SC 5 CG Diptera (true flies) Tipulidae 2 17 I BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
mm
nn
Other Total Individuals 116
(117) 999
Total Taxa 10 (11)
Notes Subsample taken 6 November 2008 Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009 Gerridae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
- Disclaimer ii
- Title Page iii
- Acknowledgements v
- Table of Contents vii
- SECTION 2 RESULTS DATA TABLES 7
- OVERVIEW AND FINDINGS
- Introduction
- RESULTS DATA TABLES
- REFERENCES
- FIELD DATA SHEETS
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- SubstrateType
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- WATER QUALITY ANALYSIS
- THROUGH THE VIRGINIA
- STREAM CONDITION INDEX (VSCI)
- LABORATORY BENCH SHEETS
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
Site Name and Code Sampling
Date of
TaxaVCSI Score
Water Quality Rating
Mouth (HLR-1) 21 May 2008 13 571 Fair (Gray Zone)
Mouth (HLR-1) 10 Oct 2008 10 608 Fair (Gray Zone)
site average for 2008 590 Fair (Gray Zone)
Package Plant (HLR-2) 29 May 2008 5 370 Severely Stressed
Package Plant (HLR-2) 10 Oct 2008 7 674 Good
site average for 2008 522 Moderately Stressed
Route 664 Bridge (HLR-3) 28 May 2008 9 485 Moderately Stressed
Route 664 Bridge (HLR-3) 10 Oct 2008 7 469 Moderately Stressed
site average for 2008 477 Moderately Stressed
Unnamed Tributary (HLR-4) 31 May 2008 7 173 Severely Stressed
Unnamed Tributary (HLR-4) 10 Oct 2008 17 476 Moderately Stressed
site average for 2008 325 Severely Stressed
Upper Lick Run (HLR-5) 29 May 2008 7 455 Moderately Stressed
site average for 2008 455 Moderately Stressed
McCann Farm (HLR-6) 30 May 2008 3 190 Severely Stressed
McCann Farm (HLR-6) 10 Oct 2008 10 316 Severely Stressed
site average for 2008 253 Severely Stressed
Summary 6 sites 11 samples
36 BMI taxa
VSCI = Virginia Stream Condition Index see Burton and Gerritsen (2003) and Virginia Department of Environmental Quality (2006b and 2008) Table 1 Summary Table of Virginia Stream Condition Index (VSCI) Scores and Water Quality Ratings for Sites in the Hiatt-Lick Run Subwatershed of Opequon Creek Frederick County Virginia 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
4
Improvements in water quality ratings at three sites between May and October suggest that BMPs and other changes may already be having some positive effects on water quality At the Package Plant (Site HLR-2) the VSCI score rose dramatically from 370 (severely stressed) to 674 (good) The small-scale sewage treatment plant as noted above was taken off-line during this period Similarly water quality at the Unnamed Tributary (HLR-4) increased from 173 (severely stressed) to 476 (moderately stressed) Adjacent construction was completed over the summer and grass was planted on the property Management practices on the McCann Farm (HLR-6) may be responsible for improving conditions for aquatic life in Hiatt Runrsquos headwaters Even though the water quality was still rated as severely stressed in October the VSCI score rose from 190 to 316 at this sampling site Further studies are needed to determine whether the changes noted in 2008 persist into the future In 2003 the DEQ began a Total Maximum Daily Load (TMDL) process for Opequon Creek as required by the Water Quality Act of 1987 The TMDL approach establishes a ldquopollution budgetrdquo for each impaired stream for each type of impairment and for each responsible pollutant In conjunction with the DEQ and the Virginia Department of Conservation and Recreation (DCR) Virginia Polytechnic Institutersquos Department of Biosystems Engineering developed a TMDL implementation plan (Opequon Creek IP Steering Committee et al 2006) Monitoring the BMI communities of Opequon Creek and its tributaries is essential to evaluating the progress of the proposed water quality improvement projects
Future Research The relatively small Hiatt-Lick Run subwatershed provides several case studies that can help assess the effectiveness of various water quality management practices not only in Opequon Creek but also in other watersheds in the Chesapeake Bay region and beyond In supporting that goal this study makes two principal contributions 1 It provides baseline data to measure the improvement or degradation of the Hiatt-Lick Run section of the Opequon Creek watershed and 2 It establishes a monitoring framework for the long-term biological monitoring of water quality and the evaluation of best management practices (BMPs) This framework consists of
bull Sampling Sites ndash six stream reaches in the Hiatt-Lick Run subwatershed selected as sampling sites according to EPA and DEQ specifications
bull Data Collection Protocols ndash based on the EPArsquos Rapid Bioassessment Protocols (RBPs)
(Barbour et al 1999) and the DEQrsquos Virginia Stream Index (VSCI) (Burton and Gerritsen 2003 DEQ 2006b and 2008) and
bull Analytical Procedures ndash contained in the Virginia Stream Condition Index (VSCI see
Burton and Gerritsen 2003 DEQ 2006b pp 8 and 33 and DEQ 2008 pp 29-34)
5
6
Section 2
RESULTS DATA TABLES
Site HLR-1 Mouth
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 13 189 6872 EPT taxa 5 117 4273 Ephemeroptera 121 477 2544 Plec +Tric less Hydropsych 154 562 2745 Scrapers 352 467 7536 Chironomidae 286 16 7267 Top 2 Dominant 550 328 6708 Hilsenhoff Biotic Index (HBI) 45 29 775VSCI Score 571
According to the DEQrsquos water quality standards aquatic life at this site is fair (gray zone) See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 2 VSCI Scores for Mouth (HLR-1) Site in Hiatt-Lick Run Sampled on 21 May 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 10 189 5292 EPT taxa 4 117 3423 Ephemeroptera 163 477 3424 Plec +Tric less Hydropsych 360 562 6415 Scrapers 221 467 4736 Chironomidae 00 16 10007 Top 2 Dominant 546 328 6768 Hilsenhoff Biotic Index (HBI) 39 29 859VSCI Score 608
According to the DEQrsquos water quality standards aquatic life at this site is fair (gray zone) See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 3 VSCI Scores for Mouth (HLR-1) Site in Hiatt-Lick Run Sampled 10 October 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
7
Site HLR-2 Package Plant
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 5 189 2642 EPT taxa 2 117 1713 Ephemeroptera 00 477 004 Plec +Tric less Hydropsych 110 562 1965 Scrapers 173 467 3706 Chironomidae 395 16 6157 Top 2 Dominant 568 328 6438 Hilsenhoff Biotic Index (HBI) 50 29 704VSCI Score 370
According to the DEQrsquos water quality standards aquatic life at this site is severely stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 4 VSCI Scores for Package Plant (HLR-2) Site in Hiatt-Lick Run Sampled 29 May 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 7 189 3702 EPT taxa 34 117 2563 Ephemeroptera 392 477 8214 Plec +Tric less Hydropsych 275 562 4895 Scrapers 539 467 10006 Chironomidae 00 16 10007 Top 2 Dominant 667 328 4968 Hilsenhoff Biotic Index (HBI) 32 29 958VSCI Score 674
According to the DEQrsquos water quality standards aquatic life at this site is good See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 5 VSCI Scores for Package Plant (HLR-2) Site in Hiatt-Lick Run Sampled 10 October 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
8
Site HLR-3 Route 664 Bridge
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 9 189 4762 EPT taxa 5 117 4273 Ephemeroptera 30 477 634 Plec +Tric less Hydropsych 61 562 1095 Scrapers 446 467 9556 Chironomidae 308 16 7037 Top 2 Dominant 723 328 4128 Hilsenhoff Biotic Index (HBI) 48 29 732VSCI Score 485
According to the DEQrsquos water quality standards aquatic life at this site is moderately stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 6 VSCI Scores for Route 664 Bridge (HLR-3) Site in Hiatt-Lick Run Sampled 28 May 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 7 189 3702 EPT taxa 4 117 3423 Ephemeroptera 40 477 844 Plec +Tric less Hydropsych 367 562 6535 Scrapers 122 467 2616 Chironomidae 00 16 10007 Top 2 Dominant 847 328 2288 Hilsenhoff Biotic Index (HBI) 42 29 817VSCI Score 469
According to the DEQrsquos water quality standards aquatic life at this site is moderately stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 7 VSCI Scores for Route 664 Bridge (HLR-3) Site in Hiatt-Lick Run Sampled 10 October 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
9
Site HLR-4 Unnamed Tributary
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 7 189 3702 EPT taxa 1 117 853 Ephemeroptera 00 477 004 Plec +Tric less Hydropsych 28 562 495 Scrapers 00 467 006 Chironomidae 847 16 1557 Top 2 Dominant 889 328 1658 Hilsenhoff Biotic Index (HBI) 60 29 563VSCI Score 173
According to the DEQrsquos water quality standards aquatic life at this site is severely stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 8 VSCI Scores for Unnamed Tributary (HLR-4) Site in Hiatt-Lick Run Sampled 31 May 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 17 189 8992 EPT taxa 3 117 2563 Ephemeroptera 16 477 344 Plec +Tric less Hydropsych 63 562 1125 Scrapers 48 467 1036 Chironomidae 127 16 8877 Top 2 Dominant 254 328 10008 Hilsenhoff Biotic Index (HBI) 63 29 521VSCI Score 476
According to the DEQrsquos water quality standards aquatic life at this site is moderately stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 9 VSCI Scores for Unnamed Tributary (HLR-4) Site in Hiatt-Lick Run Sampled 10 October 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
10
Site HLR-5 Lick Run
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 7 189 3702 EPT taxa 3 117 2563 Ephemeroptera 167 477 3504 Plec +Tric less Hydropsych 167 562 2975 Scrapers 106 467 2276 Chironomidae 348 16 6637 Top 2 Dominant 545 328 6778 Hilsenhoff Biotic Index (HBI) 43 29 803VSCI Score 455
According to the DEQrsquos water quality standards aquatic life at this site is moderately stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 10 VSCI Scores for Lick Run (HLR-5) Site in Hiatt-Lick Run Sampled 29 May 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
Note
Water conditions at the Lick Run (HLR-5) site were too low in October 2008 to permit a sample to be taken
11
Site HLR-6 McCann Farm
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 3 189 1592 EPT taxa 0 117 003 Ephemeroptera 00 477 004 Plec +Tric less Hydropsych 00 562 005 Scrapers 23 467 496 Chironomidae 00 16 10007 Top 2 Dominant 988 328 188 Hilsenhoff Biotic Index (HBI) 79 29 296VSCI Score 190
According to the DEQrsquos water quality standards aquatic life at this site is severely stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 11 VSCI Scores for McCann Farm (HLR-6) Site in Hiatt-Lick Run Sampled 30 May 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 10 189 5292 EPT taxa 3 117 2563 Ephemeroptera 51 477 1074 Plec +Tric less Hydropsych 09 562 165 Scrapers 43 467 926 Chironomidae 00 16 10007 Top 2 Dominant 887 328 1688 Hilsenhoff Biotic Index (HBI) 75 29 352VSCI Score 315
According to the DEQrsquos water quality standards aquatic life at this site is severely stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 12 VSCI Scores for McCann Farm (HLR-6) Site in Hiatt-Lick Run Sampled 10 October 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
12
REFERENCES
Barbour Michael T Jeroen Gerritsen Blaine D Snyder and James B Stribling 1999 Rapid bioassessment protocols for use in streams and wadeable rivers periphyton benthic macroinvertebrates and fish Second edition EPA 841-B-99-002 Washington DC US Environmental Protection Agency Office of Water Available online at httpwwwepagovOWOWmonitoringbioasspshtml Burton June and Jeroen Gerritsen 2003 A stream condition index for Virginia non-coastal streams Prepared for the US EPA Office of Science and Technology US EPA Region 3 Environmental Services Division and Virginia Department of Environmental Quality Owings Mills Maryland Tetra Tech Inc Available online at httpwwwdeqvirginiagovwatermonitoringpdf vastrmconpdf Hessler Rachel A Andrea R Martin Heidi D Ruggeri William B Seymour Jr and Woodward S Bousquet 2006 Water quality assessment using benthic macroinvertebrates A study and compilation Winchester Virginia Environmental Studies Program Shenandoah University Occasional publication no 10 Igoe Robert 2006 DEQ identifies nine impaired streams in Clarke Frederick The Winchester Star 15 July B1 Jackson Charlie 2005 Survey targets Opequon cleanup The Winchester Star 19 August B1 Johnston Elizabeth A and Woodward S Bousquet 2003 What happens when it rains A study of water quality in Abrams Creek and Town Run Winchester and Frederick County Virginia Winchester Virginia Environmental Studies Program Shenandoah University Occasional publication no7 Merritt Richard W and Kenneth W Cummins 1996 An introduction to the aquatic insects of North America Dubuque Iowa KendallHunt Publishing Company Opequon Creek IP Steering Committee Virginia Tech Department of Biological Systems Engineering and Center for TMDL and Watershed Studies University of Virginia Institute for Environmental Negotiation and West Virginia University Division of Resource Management 2006 Opequon Creek watershed TMDL implementation plan Blacksburg Virginia Virginia Tech Department of Biological Systems Engineering Available online at httpwwwtmdlbsevteduoutreachC82 Pennak Robert W 1953 Fresh-water invertebrates of the United States New York New York The Ronald Press Company Rosenburg David M and Vincent H Resh 1993 Freshwater biomonitoring and benthic macroinvertebrates New York New York Chapman amp Hall Shenandoah University Public Relations Office 2002 Data collected by SU and its partners helps identify impaired water Winchester Virginia Available online at httpwwwsuedutemp_ newscfmurlnum=32 Thorp James H and Alan P Covich 1991 Ecology and classification of North American freshwater invertebrates San Diego California Academic Press Inc
13
US Environmental Protection Agency 1997 Volunteer stream monitoring a methods manual EPA 841-B-97-003 Washington DC US Environmental Protection Agency Office of Water Van Meter Val 2003 Opequon Creek pollution limits to be established The Winchester Star 4 April B1 Virginia Department of Environmental Quality 2006a Virginia 305(b)303(d) water quality integrated report to Congress and the EPA Administrator for the period January 1 2000 to December 31 2004 Richmond Virginia Virginia Department of Environmental Quality Available online at httpwwwdeqvirginiagovwqair2006html __________ 2006b Using probabilistic data to validate the non-coastal Virginia Stream Condition Index (draft) VDEQ technical bulletin WQA2006-001 Virginia Department of Environmental Quality Available online at httpwwwloudounwatershedorgstream_assessmentreference_documents VA_DEQ_SCI_Validationpdf __________ 2008 Biological monitoring program quality assurance project plan for wadeable streams and rivers Richmond Virginia Virginia Department of Environmental Quality Update available online at httpwwwdeqstatevausbiomonitoringdocumentsBioMonQAPP_13Aug2008pdf Virginia Tech Aquatic Entomology Laboratory 2005 Taxa dictionary [Excel file] Blacksburg Virginia Virginia Tech Department of Biological Systems Engineering 2003a Opequon watershed TMDLs for benthic impairments Abrams Creek and Lower Opequon Creek Frederick and Clarke Counties Virginia Prepared for the Virginia Department of Environmental Quality and Virginia Department of Conservation and Recreation Blacksburg Virginia Available online at httpwwwdeqstate vaustmdlapptmdlsshenrvrabropebcpdf Voshell J Reese Jr 2002 A guide to common freshwater invertebrates of North America Blacksburg Virginia The McDonald amp Woodward Publishing Company
14
APPENDICES
15
16
a
Appendix A
FIELD DATA SHEETS
This appendix presents a Site Characterization Field Data Sheet for each of the six sampling reaches established on Hiatt Run and Lick Run for this study They were filled out during the sampling runs made in May 2008
These forms were adapted by Shenandoah University from forms used by the EPA and Virginia DEQ for recording characteristics of sampling sites
b
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Mouth STATION _HLR-1_____ STREAM CLASS LAT N 39 12749 (upstream) LONG W 078 04209(upstream) LAT N 39 12729 (downstream) LONG W 078 04148 (downstream) FORM COMPILED BY Jeremy Tovar RIVER BASIN Potomac River INVESTIGATORS JPangle B Millholland MDahl SRobertson W Bousquet JTovar BBousquet
DATE May 21 2008_________ TIME_250 PM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature __24___degC showers (intermittent) Comments _______________________
___ cloud cover _0_ ______________________________
X clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x ( Significant springs andor seeps) x (Valley ) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS
c
d
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence x FieldPasture Industrial Obvious sources Agricultural Other ____________ Stream scour steep slope erosion x Residential Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left Field and young thin deciduous forest 100 Bank=Grass cobble and shrub Right 90 maturing deciduous forest 45 shale slope Bank=Bedrock soil and herbs Indicate the dominant vegetation type(s) and record the dominant species
x Trees Shrubs x (Grasses) Forbs Dominant species Oak species and mix deciduous
INSTREAM FEATURES
Measured reach length ___100__m Canopy Cover Estimated stream width _35__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area __350_m2
Estimated stream depth _008_m Proportion of Reach Represented by Stream High water mark _15_m Morphology Types Surface velocity (at thalweg) _05__msec x Riffle__75___ x Run__20___ x Pool__5___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___00__ m2 Density of LWD _-_ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __15_____ x Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present Algae
WATER QUALITY Temperature __19___degC Water Odors x NormalNone Sewage Specific Conductance - Petroleum Chemical Fishy Other___________________ Dissolved Oxygen - Water Surface Oils Slick Sheen Globs Flecks pH __-_ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used _____NA________ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic x None Relict shells Other______None_____ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 8 Detritus sticks wood coarse plant materials
0 Boulder gt256 mm (10rsquorsquo) 0
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 51 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 40 Sand 006-2 mm (gritty) 1 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University
SITE CHARACTERIZATION FIELD DATA SHEET FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Package Plant STATION HLR-2 STREAM CLASS LAT N 39 129580 (upstream) LONG W 078 04900 (upstream) LAT N 39 129581 (downstream) LONG W 078 04822 (downstream) FORM COMPILED BY JW Pangle RIVER BASIN Potomac River INVESTIGATORS JPangle JTovar MDahl B Millholland SRobertson W Bousquet
DATE__19 May 2008_ TIME__1240__ PM
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature _____degC showers (intermittent) Comments __Sunny Day_____________
_5__ cloud cover _0__ ______________________________
x clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x (Significant springs andor seeps) x (Valley) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS
e
f
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence FieldPasture Industrial Obvious sources Agricultural Other ____________ __Upstream Construction and Stream bank Scour Residential __________________________________ Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left Deciduous forest with thick herb and shrub layers bank= vegetation and rocks Right Deciduous forest with thick herb and shrub layers bank=vegetation soil and rocks Indicate the dominant vegetation type(s) and record the dominant species
x Trees x Shrubs Grasses Forbs Dominant species Black Walnut White Ash Hackberry and American Sycamore
INSTREAM FEATURES
Measured reach length 100 m Canopy Cover Estimated stream width _35__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area __350_m2
Estimated stream depth __15__m Proportion of Reach Represented by Stream High water mark __22___m Morphology Types Surface velocity (at thalweg) _035__msec x Riffle__30___ x Run__30___ x Pool__40___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___10____ m2 Density of LWD _______ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __30___ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present__________________________________________________________
WATER QUALITY Temperature __18___degC Water Odors x NormalNone Sewage Specific Conductance __-____ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ____-____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used _____NA____ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other___None_____ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach SubstrateType Characteristic
Composition in Sampling Reach
Bedrock 3 Detritus sticks wood coarse plant materials
2 Boulder gt256 mm (10rsquorsquo) 5
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 70 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 22 Sand 006-2 mm (gritty) 0 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiattrsquos Run LOCATION Rt 664 Bridge STATION HLR-3 STREAM CLASS LAT N 39 12934 (upstream) LONG W 078 05019 (upstream) LAT N 39 12958 (downstream) LONG W 078 04958 (downstream) FORM COMPILED BY Sean Robertson RIVER BASIN Potomac River INVESTIGATORS SRobertson BMillholland WBousquet MDahl JPangle
DATE 28 May 2008 TIME 1127 AM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality 2008
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature __14___degC showers (intermittent) x Trace Comments _______________________
_65 x cloud cover ___ ______________________________
x clearsunny __________________________________
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x (Significant springs andor seeps) x (Valley) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS
g
h
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence Some evidence x FieldPasture Industrial x Obvious sources Agricultural x Other Old Package Plant Downstream x Residential Bridge and Road Power line and Pump station Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left 70 field 30 shrubs and deciduous trees Bank=Soil and vegetation Right Maturing deciduous forest steep slope Bank=Shale Bedrock Indicate the dominant vegetation type(s) and record the dominant species
x Trees x Shrubs x Grasses Forbs Dominant species Sycamore and Red Oaks
INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width __51___m Fully open x Partly open Partly shaded Fully shaded Sampling reach area _510_m2
Estimated stream depth __135___m Proportion of Reach Represented by Stream High water mark __-___m Morphology Types Surface velocity (at thalweg) __20___msec x Riffle_40_ x Run_50_ x Pool 10 Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___0____ m2 Density of LWD ___-____ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation ___50____ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present Brown Algae
WATER QUALITY Temperature __17___degC Water Odors x NormalNone Sewage Specific Conductance ___-___ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ___-_____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used ____NA_______ Clear x Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other____None____ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 15 Detritus sticks wood coarse plant materials
0 Boulder gt256 mm (10rsquorsquo) 15
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 62 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 8 Sand 006-2 mm (gritty) 0 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Unnamed Tributary STATION HLR-4 STREAM CLASS LAT N 39 13035 (upstream) LONG W 078 05129 (upstream) LAT N 39 13000 (downstream) LONG W 078 05086 (downstream) FORM COMPILED BY Marie Dahl RIVER BASIN Potomac River INVESTIGATORS M Dahl HKepple SRobertson AFisher
DATE 31 May 2008 TIME 5 PM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality 2008
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) YES x NO
rain (steady rain) Air Temperature __22___degC showers (intermittent) Comments _______________________
_0__ cloud cover _0__ ______________________________
x clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area __-___ km2 mi2 Montane Significant springs andor seeps x Valley Marsh or swamp Montane amp Valley Mixture of origins Other ___________________
COMMENTS Snowden Bridge development (upstream) and sewer line
i
j
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence x FieldPasture Industrial Obvious sources Agricultural Other ____________ Construction upstream and adjacent x Residential Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left Forested with rocky banks field across silt fence and 2m tree strip Right Forested with some rocky banks and forest through the highway Indicate the dominant vegetation type(s) and record the dominant species
x Trees x Shrubs x Grasses Forbs Dominant species White Ash and Hackberry
INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width ___38__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area __380__m2
Estimated stream depth __12__cm Proportion of Reach Represented by Stream High water mark __2___m Morphology Types Surface velocity (at thalweg) __46___msec x Riffle__25___ x Run__70___ x Pool__5___ Channelized Yes x No Dam Present x Yes No
LARGE WOODY DEBRIS
LWD _______ m2 Density of LWD _______ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation ___NA____ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present___Algae___
WATER QUALITY Temperature __16___degC Water Odors x NormalNone Sewage Specific Conductance __-____ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ___-_____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used ____NA_____ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells x Other___Shale_______ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 0 Detritus sticks wood coarse plant materials
10 Boulder gt256 mm (10rsquorsquo) 5
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 45 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 30 Sand 006-2 mm (gritty) 0 Marl grey shell
fragments 0
Silt 0004-006 mm 20 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Lick Run STATION HLR-5 STREAM CLASS LAT N 39 127517 (upstream) LONG W 078 05685(upstream) LAT N 39 12784 (downstream) LONG W 078 05631 (downstream) FORM COMPILED BY B Millholland RIVER BASIN Potomac River INVESTIGATORS B Millholland JTovar SRobertson MDahl WBousquet JPangle
DATE 29 May 2008 TIME 1040 AM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project
k
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature __15___degC showers (intermittent) Comments _______________________
_5_ X cloud cover _0__ ______________________________
x clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane Significant springs andor seeps x Valley Marsh or swamp Montane amp Valley Mixture of origins Other ___________________
COMMENTS Lick Run appears to be intermittent and may be dry in Fall 2008
l
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence x FieldPasture Industrial Obvious sources Agricultural Other ____________ Adjacent logging stream bank scour Residential Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left 85 young deciduous forest 15 logged Bank=Soil vegetation and cobbles Right Young deciduous forest on 30 slope Bank= Soil vegetation and cobble Indicate the dominant vegetation type(s) and record the dominant species
x Trees Shrubs Grasses Forbs Dominant species Mixed Deciduous INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width __11__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area 110 m2
Estimated stream depth _009__m Proportion of Reach Represented by Stream High water mark __96___m Morphology Types Surface velocity (at thalweg) __01___msec x Riffle__30___ x Run__5___ x Pool__65___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___0____ m2 Density of LWD ___-____ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __70___ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present__________________________________________________________
WATER QUALITY Temperature __14___degC Water Odors x NormalNone Sewage Specific Conductance __-__ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ___-___ Water Surface Oils Slick Sheen Globs Flecks pH __-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used _____NA______ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other_____None_____
SEDIMENT AND SUBSTRATE
Odors Deposits Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other________________ x Other Slightly Salty Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 0 Detritus sticks wood coarse plant materials
5 Boulder gt256 mm (10rsquorsquo) 0
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 75 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 23 Sand 006-2 mm (gritty) 2 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION McCann Farm STATION HLR-6 STREAM CLASS LAT N 39 13076 (upstream) LONG W 078 06539 (upstream) LAT N 39 13063 (downstream) LONG W 078 06473 (downstream) FORM COMPILED BY MDahl RIVER BASIN Potomac River INVESTIGATORS MDahl JPangle SRobertson W Bousqet BMillholland JTovar H Kepple
DATE 30 May 2008 TIME 1050 AM (start)
PROJECT NAME
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) YES x NO
rain (steady rain) Air Temperature __18___degC showers (intermittent) Comments _______________________
_85_x cloud cover _10__ ______________________________
clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x (Significant springs andor seeps) x ( Valley) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS Multi-habitat sampling with D-frame nets and seine
m
n
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial x No evidence Some evidence x FieldPasture Industrial Obvious sources x Agricultural Other ____________ Minor stream bank scour Residential Local Watershed Erosion x None Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left 35 bank grasses scattered trees and some bushes Right High bank with grasses and scattered trees Indicate the dominant vegetation type(s) and record the dominant species
x Trees Shrubs Grasses Forbs Dominant species Black Walnut and Festuca elatior
INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width __2___m Fully open Partly open x Partly shaded Fully shaded Sampling reach area __200_m2
Estimated stream depth __03_m Proportion of Reach Represented by Stream High water mark __1___m Morphology Types Surface velocity (at thalweg) __166_msec x Riffle__0___ x Run__55___ x Pool__45___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___2____ m2 Density of LWD ___-____ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __20_____ x Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present ____Glyceria (grass)sp__________
WATER QUALITY Temperature __16___degC Water Odors x NormalNone Sewage Specific Conductance ___-___ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ____-____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used ______NA___ Clear x Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other__NONE__ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 0 Detritus sticks wood coarse plant materials
5 Boulder gt256 mm (10rsquorsquo) 0
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 27 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 38 Sand 006-2 mm (gritty) 5 Marl grey shell
fragments 0 (lt1)
Silt 0004-006 mm 25 Clay lt 0004 mm (slick) 5 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Appendix B
WATER QUALITY ANALYSIS THROUGH THE VIRGINIA
STREAM CONDITION INDEX (VSCI)
The Virginia Stream Condition Index (VSCI) relies on eight metrics to categorize stream health According to Burton and Gerritsen (2003 p 1-1) this index ldquohellip can be used as a primary indicator of ecosystem health and can identify impairment with respect to the reference (or natural) conditionrdquo These metrics are numeric measures of a biological communityrsquos species composition diversity ecological niches or tolerance to pollution
Table 13 on the next page describes each VSCI metric lists its formula and provides the best standard value (BSV) for that metric Based on a 100-point scale the BSV is the score that reflects optimum conditions for aquatic life ie the best water quality value possible (DEQ 2006b pp 8 33) The table also explains whether the score increases or decreases with environmental stress
As Table 13 indicates each of the eight VSCI metrics is calculated individually Each formula provides a unitless score based on a 100-point scale with 0 as the worst possible score and 100 as the best Any metric that provides a result above 100 is changed to 100 in order for the index to work properly After the eight metrics are calculated they are summed and averaged to provide a final VSCI score on a 100-point scale
Based on statistical analysis of Virginiarsquos streams and their BMI communities the DEQ (2006b p 8) currently uses the following tiers to interpret VSCI scores
VSCI Scores Aquatic Life Use
(ALI) Tiers
lt 42 severely stressed
42-55 moderately stressed
55-63 unknownfair (gray zone)
63-73 good
gt73 excellent
Section 2 of this report presents the results of calculations for the six BMI sampling sites the research team studied in the Hiatt-Lick Run subwatershed in 2008
o
p
1 Total taxa the number of distinct taxa in the sample measures the overall diversity of the macroinvertebrate assemblage The best standard value (BSV) is 189 Score = 100 x (X189) Total taxa decreases with stress 2 EPT taxa the sum of the distinct taxa in the orders Ephemeroptera (mayflies) Plecoptera (stoneflies) and Trichoptera (caddisflies) These orders are known to be sensitive to pollution The best standard value (BSV) is 117 Score = 100 x (X117) EPT taxa decreases with stress 3 Percent Ephemeroptera the percent abundance of individuals in the sample that belong to the order Ephemeroptera (mayflies) The best standard value (BSV) is 477 Score = 100 x (X477) Percent Ephemeroptera decreases with stress 4 Percent Plecoptera + Trichoptera less Hydropsychidae the percent abundance of individuals in the sample that belong to the orders Plecoptera (stoneflies) and Trichoptera (caddisflies) excluding the pollution-tolerant caddisfly family Hydropsychidae The best standard value (BSV) is 562 Score = 100 x (X562) Percent Plecoptera + Trichoptera less Hydropsychidae decreases with stress 5 Percent Scrapers the percent abundance of individuals in the sample whose primary functional mechanism for feeding is grazing on the substrate or periphyton (ie attached algae and associated material) The best standard value (BSV) is 467 Score = 100 x (X467) Percent scrapers decreases with stress 6 Percent Chironomidae the percent abundance of individuals in the sample that are Chironomidae (non-biting midge) larvae and pupae The best standard value (BSV) is 16 Score = 100 x [(100-X)(100-16)] Percent Chironomidae increases with stress 7 Percent Top Two Dominant the percent abundance of the two most frequent taxa present in the sample The best standard value (BSV) is 328 Score = 100 x [(100-X)(100-328)] The Percent Top 2 Dominant increases with stress 8 Hilsenhoff Biotic Index (HBI) an abundance-weighted average pollution tolerance of all the individuals within a sample keyed to the family level Tolerance ratings range from 1 (highly intolerant) to 10 (highly tolerant) The best standard value (BSV) is 29 Score = 100 x [(10-X)(10-29)] The Hilsenhoff Biotic Index (HBI) increases with stress ______________________________________________________________________________ Table 13 Description of Benthic Macroinvertebrate VSCI Metrics Includes responses (increases decreases) of each VSCI metric to degraded water quality conditions Adapted from Burton and Gerritsen (2003 pp 3-15 3-16 6-2) and DEQ (2008)
q
Appendix C
LABORATORY BENCH SHEETS
Shenandoah Universityrsquos Environmental Studies Department developed the Benthic Macroinvertebrate Laboratory Bench Sheet and VSCI Worksheet to record BMI specimen identifications and their VSCI classifications for each sample
This form is based in part on bench sheets developed by the EPA and Virginia DEQ for recording specimen identifications Additional columns on Shenandoah Universityrsquos forms for relative numbers and VSCI classifications make the calculation and verification of VSCI scores easier
Note the column for taxonomistrsquos initials Specimens were identified by students in Shenandoah Universityrsquos research group and then verified or where necessary changed by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality This external review helps assure the validity of the study Originals of these bench sheets with handwritten taxonomistsrsquo initials will be kept on file at Shenandoah Universityrsquos Environmental Studies Department for five years after the completion of this study
r
s
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-1 STATION NAME Mouth LAT N 39deg 12749rsquo (upstream) LONG W 078deg 04209rsquo (upstream) LAT N 39deg 12729rsquo (downstream) LONG W 078deg 04148rsquo (downstream) FORM COMPILED BY Jeremy Tovar W S Bousquet RIVER BASIN Potomac River INVESTIGATORS Marie Dahl Brandon Millholand J W Pangle Sean Robertson Jeremy Tovar Woodward Bousquet Ben Bousquet
Collection date 21 May 2008 Sheet completion date 12 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Hirudinea 3 33 JT BVW PR 8 SP Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Coenagrionidae 2 22 BVW PR 9 CR Ephemeroptera (mayflies) Caenidae 1 11 JT BVW CG 6 SP Ephemerllidae 4 44 JT BVW CG 2 CR Heptageniidae 5 55 JT BVW SC 3 CG Isonychiidae 1 11 BVW CF 3 CG Plecoptera (stoneflies) Perlidae 14 154 JT BVW PR 2 CR Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Corydalidae 1 11 JT BVW PR 5 CR Coleoptera (beetles) Elmidae 24 264 I JT BVW SC 4 CG Dytiscidae 4 44 JT BVW PR 6 GN Psephenidae 3 33 JT BVW SC 4 CG Diptera (true flies) Tipulidae 3 33 JT BVW SH 4 BU Chironomidae 26 286 JT BVW CG 6 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
t
Other Total Individuals 91 1001 Total Taxa 13
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
u
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-1 STATION NAME Mouth LAT N 39deg 12749rsquo (upstream) LONG W 078deg 04209rsquo (upstream) LAT N 39deg 12729rsquo (downstream) LONG W 078deg 04148rsquo (downstream) FORM COMPILED BY Jeremy Tovar W S Bousquet RIVER BASIN Potomac River INVESTIGATORS Brandon Millholand JW Pangle Jeremy Tovar Heather Kepple
Collection date 10 October 2008 Sheet completion date 12 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Oligochaeta 3 35 JT BVW CG 8 BU Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Coenagrionidae 2 23 JT BVW PR 9 CR Ephemeroptera (mayflies) Heptageniidae 12 140 JT BVW SC 3 CG Isonychiidae 2 23 BVW CF 3 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Hydropsychidae 16 186 JT BVW CF 6 CG Philopotamidae 31 360 JT BVW CF 2 CG Hemiptera (true bugs) (Veliidae) (5) - JT BVW ( ) Megaloptera (dobsonflies etc) Corydalidae 10 116 JT BVW PR 5 CR Coleoptera (beetles) Dytiscidae 1 12 JT BVW PR 6 GN Psephenidae 7 81 JT BVW SC 4 CG Diptera (true flies) Tipulidae 2 23 BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
v
Other Total Individuals 86
(91) 999
Total Taxa 10 (11)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009 Veliidae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
w
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-2 STATION NAME Package Plant LAT N 39deg 12958rsquo (upstream) LONG W 078deg 04900 (upstream) LAT N 39deg 12958rsquo (downstream) LONG W 078deg 04822 (downstream) FORM COMPILED BY JW Pangle W Bousquet RIVER BASIN Potomac River INVESTIGATORS JW Pangle S Robertson J Tovar M Dahl B Millholland W Bousquet
Collection date 29 May 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Plecoptera (stoneflies) Perlidae 11 136 I JP BVW PR 1 CR Trichoptera (caddisflies) Hydropsychidae 10 123 I JP BVW CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 14 173 I JP BVW SC 4 CG Diptera (true flies) Chironomidae 32 395 I JP BVW CG 6 BU Simuliidae 14 173 I JP BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
x
Other Total Individuals 81 1000 Total Taxa 5
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-2 STATION NAME Package Plant LAT N 39deg 12958rsquo (upstream) LONG W 078deg 04900 (upstream) LAT N 39deg 12958rsquo (downstream) LONG W 078deg 04822 (downstream) FORM COMPILED BY JW Pangle W Bousquet RIVER BASIN Potomac River INVESTIGATORS JW Pangle Brandon Millholland Jeremy Tovar Heather Kepple
Collection date 10 October 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Heptageniidae 40 392 I WBJP SC 3 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Hydropsychidae 7 69 I JP WB BVW CF 6 CG Philopotamidae 28 275 I JP WB BVW CF 2 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Corydalidae 6 59 I JP WB BVW PR 5 CR Coleoptera (beetles) Psephenidae 7 69 I JP WB BVW SC 4 CG Elmidae 8 78 I JP WB BVW SC 4 CG Diptera (true flies) Tipulidae 6 59 I JP WB BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
y
z
Other Total Individuals 102 1001 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-3 STATION NAME Rt 664 Bridge LAT N 39deg 12934rsquo (upstream) LONG W 078deg 05019rsquo (upstream) LAT N 39deg 12951rsquo (downstream) LONG W 078deg 04958rsquo (downstream) FORM COMPILED BY S Robertson W Bousquet RIVER BASIN Potomac River INVESTIGATORS SRobertson BMillholland WBousquet MDahl JPangle
Collection date 28 May 2008 Sheet completion date 11 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Leptophlebiidae 1 15 I SGR BVW CG 2 CR Baetidae 1 15 I BVW CG 5 CG Plecoptera (stoneflies) Perlidae 3 46 I SGR BVW PR 1 CR Nemouridae 1 15 I BVW SH 3 CR Trichoptera (caddisflies) Hydropsychidae 5 77 I SGR BVW CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 27 415 I SGR BVW SC 4 CG Psephenidae 2 31 A SGR BVW SC 4 CG Diptera (true flies) Simuliidae 5 77 I SGR BVW CF 6 CG Chironomidae 20 308 I SGR BVW CG 6 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
aa
bb
Other Total Individuals 65 999 Total Taxa 9
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-3 STATION NAME Rt 664 Bridge LAT N 39deg 12934rsquo (upstream) LONG W 078deg 05019rsquo (upstream) LAT N 39deg 12951rsquo (downstream) LONG W 078deg 04958rsquo (downstream) FORM COMPILED BY S Robertson RIVER BASIN Potomac River INVESTIGATORS S Robertson M Dahl A Fisher H Kepple
Collection date 10 Oct 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Heptageniidae 2 20 I SGR SC 3 CG Isonychiidae 2 20 I BVW CF 2 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Philopotamidae 36 367 I SGR CF 2 CG Hydropsychidae 47 480 I SGR CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 2 20 A SGR SC 4 CG Psephenidae 8 82 A SGR SC 4 CG Diptera (true flies) Tabanidae 1 10 I SGR PR 7 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
cc
dd
Other Total Individuals 98 999 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-4 STATION NAME Unnamed Tributary LAT (upstream) N 39deg 13035rsquo LONG (upstream) W 078deg 05129rsquo LAT (downstream) N 39deg 13000rsquo LONG (downstream) W 078deg 5086rsquo FORM COMPILED BY A Fisher M Dahl rev W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson A Fisher H Kepple
Collection date 31 May 2008 Sheet completion date rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Flatworms (Turbellaria) Worms (Oligochaeta) Naididae 1 14 - BVW CG 8 BU Leeches (Hirudinea) Snails (Gastropoda) Clams (Bivalvia) Isopods (Isopoda) Asellidae 3 42 A AF BVW CG 8 SP Scuds (Amphipoda) Crayfish Shrimp (Decapoda) Water Mites (Hydracarina) Dragonflies (Odonata Anisoptera) Damselflies (Odonata Zygoptera) Mayflies (Ephemeroptera) Stoneflies (Plecoptera) Perlidae 2 28 I AF BVW PR 1 CR Caddisflies (Trichoptera) True Bugs (Hemiptera) Corixidae 1 14 I AF BVW MP 9 DV Dobsonflies (Megaloptera) Beetles (Coleoptera) Dytiscidae 1 14 I AF BVW PR 6 GN True Flies (Diptera) Chironomidae 61 847 I AF BVW CG 6 BU Simuliidae 3 42 I AF BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
ee
ff
Other Total Individuals 72 1001 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-4 STATION NAME Unnamed Tributary LAT (upstream) N 39deg 13035rsquo LONG (upstream) W 078deg 05129rsquo LAT (downstream) N 39deg 13000rsquo LONG (downstream) W 078deg 5086rsquo FORM COMPILED BY Andrew G M Fisher W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson A Fisher H Kepple
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Flatworms (Turbellaria) Worms (Oligochaeta) Lumbriculidae 2 32 A AF BVW CG 8 BU Leeches (Hirudinea) Snails (Gastropoda) Lymnaeidae 1 16 A BVW CG 9 GN Clams (Bivalvia) Isopods (Isopoda) Scuds (Amphipoda) Crayfish Shrimp (Decapoda) Cambaridae 1 16 A AF BVW GN 5 GN Water Mites (Hydracarina) Dragonflies (Odonata Anisoptera) Damselflies (Odonata Zygoptera) Calopterygidae 4 63 I BVW PR 7 CL Mayflies (Ephemeroptera) Tricorythidae 1 16 I BVW CG 5 SP Stoneflies (Plecoptera) Caddisflies (Trichoptera) Phryganeidae 4 63 I AF BVW SH 4 CR Hydropsychidae 6 95 I AF BVW CF 6 CG I True Bugs (Hemiptera) Corixidae 8 127 I AF BVW MP 9 DV (Veliidae ) (7) - I AF BVW ( ) Dobsonflies (Megaloptera) Sialidae 3 48 I AF BVW PR 7 SP Corydalidae 2 32 I AF BVW PR 5 CR Beetles (Coleoptera) Haliplidae 1 16 A AF BVW MP 8 GN Dysticidae 7 111 I AF BVW PR 6 GN Hydrophilidae 1 16 A AF BVW PR 8 GN Elmidae 3 48 I BVW SC 4 CG True Flies (Diptera) Chironomidae 8 127 I AF BVW CG 6 BU Simuliidae 7 111 I AF BVW CF 6 CG Tipulidae 4 63 I AF BVW SH 4 GU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
gg
hh
Other Total Individuals 63
(70) 1000
Total Taxa 17 (18)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Veliidae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-5 STATION NAME Lick Run LAT N 39deg 12752rsquo (upstream) LONG W 078deg 05685rsquo (upstream) LAT N 39deg 12784rsquo (downstream) LONG W 078deg 05631rsquo (downstream) FORM COMPILED BY Brandon Millholland W Bousquet RIVER BASIN Potomac River INVESTIGATORS J Pangle S Robertson W Bousquet JTovar M Dahl
Collection date 29 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Gomphidae 1 15 I BVW PR 3 BU Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Leptophlebiidae 11 167 A LBM BVW CG 2 CR Plecoptera (stoneflies) Perlidae 10 152 A LBM BVW PR 1 CR Perlodiae 1 15 A LBM BVW PR 2 CR Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 5 76 A LBM BVW SC 4 CG Elmidae 2 30 I LBM BVW SC 4 CG Diptera (true flies) (Culicidae) (3) - P LMB BVW ( ) Chironomidae 23 348 I LBM BVW CG 6 BU Simuliidae 13 197 I LBM BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
ii
jj
Other Total Individuals 66
(69) 1000
Total Taxa 7 (8)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Culicidae (Diptera) pupae not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl J Pangle S Robertson W Bousquet B Millholland J Tovar H Kepple
Collection date 30 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 1 12 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Asellidae 114 965 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Plecoptera (stoneflies) Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 2 23 A MRD BVW SC 4 CG Diptera (true flies)
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
kk
ll
Other Total Individuals 117 1000 Total Taxa 3
Notes Subsample taken 29 October 2009 All taxonomic determinations by MRD were confirmed by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson K McCann A Fisher H Kepple A Barrett
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 2 17 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Sphaeriidae 2 17 I MRD BVW CF 8 BU Isopoda (isopods) Asellidae 99 853 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Calopterygidae 2 17 A MRD BVW PR 7 CL Ephemeroptera (mayflies) Leptophlebiidae 2 17 I MRD BVW CG 2 CR Heptageniidae 4 34 A MRD BVW SC 3 CG Plecoptera (stoneflies) Perlidae 1 09 A MRD BVW PR 1 CR Trichoptera (caddisflies) Hemiptera (true bugs) (Gerridae) (1) - A MRD BVW ( ) Corixidae 1 09 A MRD BVW PR 5 CR Megaloptera (dobsonflies etc) Coleoptera (beetles) Dryopidae 1 09 A MRD BVW SC 5 CG Diptera (true flies) Tipulidae 2 17 I BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
mm
nn
Other Total Individuals 116
(117) 999
Total Taxa 10 (11)
Notes Subsample taken 6 November 2008 Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009 Gerridae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
- Disclaimer ii
- Title Page iii
- Acknowledgements v
- Table of Contents vii
- SECTION 2 RESULTS DATA TABLES 7
- OVERVIEW AND FINDINGS
- Introduction
- RESULTS DATA TABLES
- REFERENCES
- FIELD DATA SHEETS
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- SubstrateType
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- WATER QUALITY ANALYSIS
- THROUGH THE VIRGINIA
- STREAM CONDITION INDEX (VSCI)
- LABORATORY BENCH SHEETS
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
Improvements in water quality ratings at three sites between May and October suggest that BMPs and other changes may already be having some positive effects on water quality At the Package Plant (Site HLR-2) the VSCI score rose dramatically from 370 (severely stressed) to 674 (good) The small-scale sewage treatment plant as noted above was taken off-line during this period Similarly water quality at the Unnamed Tributary (HLR-4) increased from 173 (severely stressed) to 476 (moderately stressed) Adjacent construction was completed over the summer and grass was planted on the property Management practices on the McCann Farm (HLR-6) may be responsible for improving conditions for aquatic life in Hiatt Runrsquos headwaters Even though the water quality was still rated as severely stressed in October the VSCI score rose from 190 to 316 at this sampling site Further studies are needed to determine whether the changes noted in 2008 persist into the future In 2003 the DEQ began a Total Maximum Daily Load (TMDL) process for Opequon Creek as required by the Water Quality Act of 1987 The TMDL approach establishes a ldquopollution budgetrdquo for each impaired stream for each type of impairment and for each responsible pollutant In conjunction with the DEQ and the Virginia Department of Conservation and Recreation (DCR) Virginia Polytechnic Institutersquos Department of Biosystems Engineering developed a TMDL implementation plan (Opequon Creek IP Steering Committee et al 2006) Monitoring the BMI communities of Opequon Creek and its tributaries is essential to evaluating the progress of the proposed water quality improvement projects
Future Research The relatively small Hiatt-Lick Run subwatershed provides several case studies that can help assess the effectiveness of various water quality management practices not only in Opequon Creek but also in other watersheds in the Chesapeake Bay region and beyond In supporting that goal this study makes two principal contributions 1 It provides baseline data to measure the improvement or degradation of the Hiatt-Lick Run section of the Opequon Creek watershed and 2 It establishes a monitoring framework for the long-term biological monitoring of water quality and the evaluation of best management practices (BMPs) This framework consists of
bull Sampling Sites ndash six stream reaches in the Hiatt-Lick Run subwatershed selected as sampling sites according to EPA and DEQ specifications
bull Data Collection Protocols ndash based on the EPArsquos Rapid Bioassessment Protocols (RBPs)
(Barbour et al 1999) and the DEQrsquos Virginia Stream Index (VSCI) (Burton and Gerritsen 2003 DEQ 2006b and 2008) and
bull Analytical Procedures ndash contained in the Virginia Stream Condition Index (VSCI see
Burton and Gerritsen 2003 DEQ 2006b pp 8 and 33 and DEQ 2008 pp 29-34)
5
6
Section 2
RESULTS DATA TABLES
Site HLR-1 Mouth
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 13 189 6872 EPT taxa 5 117 4273 Ephemeroptera 121 477 2544 Plec +Tric less Hydropsych 154 562 2745 Scrapers 352 467 7536 Chironomidae 286 16 7267 Top 2 Dominant 550 328 6708 Hilsenhoff Biotic Index (HBI) 45 29 775VSCI Score 571
According to the DEQrsquos water quality standards aquatic life at this site is fair (gray zone) See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 2 VSCI Scores for Mouth (HLR-1) Site in Hiatt-Lick Run Sampled on 21 May 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 10 189 5292 EPT taxa 4 117 3423 Ephemeroptera 163 477 3424 Plec +Tric less Hydropsych 360 562 6415 Scrapers 221 467 4736 Chironomidae 00 16 10007 Top 2 Dominant 546 328 6768 Hilsenhoff Biotic Index (HBI) 39 29 859VSCI Score 608
According to the DEQrsquos water quality standards aquatic life at this site is fair (gray zone) See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 3 VSCI Scores for Mouth (HLR-1) Site in Hiatt-Lick Run Sampled 10 October 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
7
Site HLR-2 Package Plant
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 5 189 2642 EPT taxa 2 117 1713 Ephemeroptera 00 477 004 Plec +Tric less Hydropsych 110 562 1965 Scrapers 173 467 3706 Chironomidae 395 16 6157 Top 2 Dominant 568 328 6438 Hilsenhoff Biotic Index (HBI) 50 29 704VSCI Score 370
According to the DEQrsquos water quality standards aquatic life at this site is severely stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 4 VSCI Scores for Package Plant (HLR-2) Site in Hiatt-Lick Run Sampled 29 May 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 7 189 3702 EPT taxa 34 117 2563 Ephemeroptera 392 477 8214 Plec +Tric less Hydropsych 275 562 4895 Scrapers 539 467 10006 Chironomidae 00 16 10007 Top 2 Dominant 667 328 4968 Hilsenhoff Biotic Index (HBI) 32 29 958VSCI Score 674
According to the DEQrsquos water quality standards aquatic life at this site is good See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 5 VSCI Scores for Package Plant (HLR-2) Site in Hiatt-Lick Run Sampled 10 October 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
8
Site HLR-3 Route 664 Bridge
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 9 189 4762 EPT taxa 5 117 4273 Ephemeroptera 30 477 634 Plec +Tric less Hydropsych 61 562 1095 Scrapers 446 467 9556 Chironomidae 308 16 7037 Top 2 Dominant 723 328 4128 Hilsenhoff Biotic Index (HBI) 48 29 732VSCI Score 485
According to the DEQrsquos water quality standards aquatic life at this site is moderately stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 6 VSCI Scores for Route 664 Bridge (HLR-3) Site in Hiatt-Lick Run Sampled 28 May 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 7 189 3702 EPT taxa 4 117 3423 Ephemeroptera 40 477 844 Plec +Tric less Hydropsych 367 562 6535 Scrapers 122 467 2616 Chironomidae 00 16 10007 Top 2 Dominant 847 328 2288 Hilsenhoff Biotic Index (HBI) 42 29 817VSCI Score 469
According to the DEQrsquos water quality standards aquatic life at this site is moderately stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 7 VSCI Scores for Route 664 Bridge (HLR-3) Site in Hiatt-Lick Run Sampled 10 October 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
9
Site HLR-4 Unnamed Tributary
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 7 189 3702 EPT taxa 1 117 853 Ephemeroptera 00 477 004 Plec +Tric less Hydropsych 28 562 495 Scrapers 00 467 006 Chironomidae 847 16 1557 Top 2 Dominant 889 328 1658 Hilsenhoff Biotic Index (HBI) 60 29 563VSCI Score 173
According to the DEQrsquos water quality standards aquatic life at this site is severely stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 8 VSCI Scores for Unnamed Tributary (HLR-4) Site in Hiatt-Lick Run Sampled 31 May 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 17 189 8992 EPT taxa 3 117 2563 Ephemeroptera 16 477 344 Plec +Tric less Hydropsych 63 562 1125 Scrapers 48 467 1036 Chironomidae 127 16 8877 Top 2 Dominant 254 328 10008 Hilsenhoff Biotic Index (HBI) 63 29 521VSCI Score 476
According to the DEQrsquos water quality standards aquatic life at this site is moderately stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 9 VSCI Scores for Unnamed Tributary (HLR-4) Site in Hiatt-Lick Run Sampled 10 October 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
10
Site HLR-5 Lick Run
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 7 189 3702 EPT taxa 3 117 2563 Ephemeroptera 167 477 3504 Plec +Tric less Hydropsych 167 562 2975 Scrapers 106 467 2276 Chironomidae 348 16 6637 Top 2 Dominant 545 328 6778 Hilsenhoff Biotic Index (HBI) 43 29 803VSCI Score 455
According to the DEQrsquos water quality standards aquatic life at this site is moderately stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 10 VSCI Scores for Lick Run (HLR-5) Site in Hiatt-Lick Run Sampled 29 May 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
Note
Water conditions at the Lick Run (HLR-5) site were too low in October 2008 to permit a sample to be taken
11
Site HLR-6 McCann Farm
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 3 189 1592 EPT taxa 0 117 003 Ephemeroptera 00 477 004 Plec +Tric less Hydropsych 00 562 005 Scrapers 23 467 496 Chironomidae 00 16 10007 Top 2 Dominant 988 328 188 Hilsenhoff Biotic Index (HBI) 79 29 296VSCI Score 190
According to the DEQrsquos water quality standards aquatic life at this site is severely stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 11 VSCI Scores for McCann Farm (HLR-6) Site in Hiatt-Lick Run Sampled 30 May 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 10 189 5292 EPT taxa 3 117 2563 Ephemeroptera 51 477 1074 Plec +Tric less Hydropsych 09 562 165 Scrapers 43 467 926 Chironomidae 00 16 10007 Top 2 Dominant 887 328 1688 Hilsenhoff Biotic Index (HBI) 75 29 352VSCI Score 315
According to the DEQrsquos water quality standards aquatic life at this site is severely stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 12 VSCI Scores for McCann Farm (HLR-6) Site in Hiatt-Lick Run Sampled 10 October 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
12
REFERENCES
Barbour Michael T Jeroen Gerritsen Blaine D Snyder and James B Stribling 1999 Rapid bioassessment protocols for use in streams and wadeable rivers periphyton benthic macroinvertebrates and fish Second edition EPA 841-B-99-002 Washington DC US Environmental Protection Agency Office of Water Available online at httpwwwepagovOWOWmonitoringbioasspshtml Burton June and Jeroen Gerritsen 2003 A stream condition index for Virginia non-coastal streams Prepared for the US EPA Office of Science and Technology US EPA Region 3 Environmental Services Division and Virginia Department of Environmental Quality Owings Mills Maryland Tetra Tech Inc Available online at httpwwwdeqvirginiagovwatermonitoringpdf vastrmconpdf Hessler Rachel A Andrea R Martin Heidi D Ruggeri William B Seymour Jr and Woodward S Bousquet 2006 Water quality assessment using benthic macroinvertebrates A study and compilation Winchester Virginia Environmental Studies Program Shenandoah University Occasional publication no 10 Igoe Robert 2006 DEQ identifies nine impaired streams in Clarke Frederick The Winchester Star 15 July B1 Jackson Charlie 2005 Survey targets Opequon cleanup The Winchester Star 19 August B1 Johnston Elizabeth A and Woodward S Bousquet 2003 What happens when it rains A study of water quality in Abrams Creek and Town Run Winchester and Frederick County Virginia Winchester Virginia Environmental Studies Program Shenandoah University Occasional publication no7 Merritt Richard W and Kenneth W Cummins 1996 An introduction to the aquatic insects of North America Dubuque Iowa KendallHunt Publishing Company Opequon Creek IP Steering Committee Virginia Tech Department of Biological Systems Engineering and Center for TMDL and Watershed Studies University of Virginia Institute for Environmental Negotiation and West Virginia University Division of Resource Management 2006 Opequon Creek watershed TMDL implementation plan Blacksburg Virginia Virginia Tech Department of Biological Systems Engineering Available online at httpwwwtmdlbsevteduoutreachC82 Pennak Robert W 1953 Fresh-water invertebrates of the United States New York New York The Ronald Press Company Rosenburg David M and Vincent H Resh 1993 Freshwater biomonitoring and benthic macroinvertebrates New York New York Chapman amp Hall Shenandoah University Public Relations Office 2002 Data collected by SU and its partners helps identify impaired water Winchester Virginia Available online at httpwwwsuedutemp_ newscfmurlnum=32 Thorp James H and Alan P Covich 1991 Ecology and classification of North American freshwater invertebrates San Diego California Academic Press Inc
13
US Environmental Protection Agency 1997 Volunteer stream monitoring a methods manual EPA 841-B-97-003 Washington DC US Environmental Protection Agency Office of Water Van Meter Val 2003 Opequon Creek pollution limits to be established The Winchester Star 4 April B1 Virginia Department of Environmental Quality 2006a Virginia 305(b)303(d) water quality integrated report to Congress and the EPA Administrator for the period January 1 2000 to December 31 2004 Richmond Virginia Virginia Department of Environmental Quality Available online at httpwwwdeqvirginiagovwqair2006html __________ 2006b Using probabilistic data to validate the non-coastal Virginia Stream Condition Index (draft) VDEQ technical bulletin WQA2006-001 Virginia Department of Environmental Quality Available online at httpwwwloudounwatershedorgstream_assessmentreference_documents VA_DEQ_SCI_Validationpdf __________ 2008 Biological monitoring program quality assurance project plan for wadeable streams and rivers Richmond Virginia Virginia Department of Environmental Quality Update available online at httpwwwdeqstatevausbiomonitoringdocumentsBioMonQAPP_13Aug2008pdf Virginia Tech Aquatic Entomology Laboratory 2005 Taxa dictionary [Excel file] Blacksburg Virginia Virginia Tech Department of Biological Systems Engineering 2003a Opequon watershed TMDLs for benthic impairments Abrams Creek and Lower Opequon Creek Frederick and Clarke Counties Virginia Prepared for the Virginia Department of Environmental Quality and Virginia Department of Conservation and Recreation Blacksburg Virginia Available online at httpwwwdeqstate vaustmdlapptmdlsshenrvrabropebcpdf Voshell J Reese Jr 2002 A guide to common freshwater invertebrates of North America Blacksburg Virginia The McDonald amp Woodward Publishing Company
14
APPENDICES
15
16
a
Appendix A
FIELD DATA SHEETS
This appendix presents a Site Characterization Field Data Sheet for each of the six sampling reaches established on Hiatt Run and Lick Run for this study They were filled out during the sampling runs made in May 2008
These forms were adapted by Shenandoah University from forms used by the EPA and Virginia DEQ for recording characteristics of sampling sites
b
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Mouth STATION _HLR-1_____ STREAM CLASS LAT N 39 12749 (upstream) LONG W 078 04209(upstream) LAT N 39 12729 (downstream) LONG W 078 04148 (downstream) FORM COMPILED BY Jeremy Tovar RIVER BASIN Potomac River INVESTIGATORS JPangle B Millholland MDahl SRobertson W Bousquet JTovar BBousquet
DATE May 21 2008_________ TIME_250 PM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature __24___degC showers (intermittent) Comments _______________________
___ cloud cover _0_ ______________________________
X clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x ( Significant springs andor seeps) x (Valley ) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS
c
d
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence x FieldPasture Industrial Obvious sources Agricultural Other ____________ Stream scour steep slope erosion x Residential Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left Field and young thin deciduous forest 100 Bank=Grass cobble and shrub Right 90 maturing deciduous forest 45 shale slope Bank=Bedrock soil and herbs Indicate the dominant vegetation type(s) and record the dominant species
x Trees Shrubs x (Grasses) Forbs Dominant species Oak species and mix deciduous
INSTREAM FEATURES
Measured reach length ___100__m Canopy Cover Estimated stream width _35__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area __350_m2
Estimated stream depth _008_m Proportion of Reach Represented by Stream High water mark _15_m Morphology Types Surface velocity (at thalweg) _05__msec x Riffle__75___ x Run__20___ x Pool__5___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___00__ m2 Density of LWD _-_ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __15_____ x Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present Algae
WATER QUALITY Temperature __19___degC Water Odors x NormalNone Sewage Specific Conductance - Petroleum Chemical Fishy Other___________________ Dissolved Oxygen - Water Surface Oils Slick Sheen Globs Flecks pH __-_ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used _____NA________ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic x None Relict shells Other______None_____ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 8 Detritus sticks wood coarse plant materials
0 Boulder gt256 mm (10rsquorsquo) 0
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 51 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 40 Sand 006-2 mm (gritty) 1 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University
SITE CHARACTERIZATION FIELD DATA SHEET FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Package Plant STATION HLR-2 STREAM CLASS LAT N 39 129580 (upstream) LONG W 078 04900 (upstream) LAT N 39 129581 (downstream) LONG W 078 04822 (downstream) FORM COMPILED BY JW Pangle RIVER BASIN Potomac River INVESTIGATORS JPangle JTovar MDahl B Millholland SRobertson W Bousquet
DATE__19 May 2008_ TIME__1240__ PM
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature _____degC showers (intermittent) Comments __Sunny Day_____________
_5__ cloud cover _0__ ______________________________
x clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x (Significant springs andor seeps) x (Valley) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS
e
f
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence FieldPasture Industrial Obvious sources Agricultural Other ____________ __Upstream Construction and Stream bank Scour Residential __________________________________ Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left Deciduous forest with thick herb and shrub layers bank= vegetation and rocks Right Deciduous forest with thick herb and shrub layers bank=vegetation soil and rocks Indicate the dominant vegetation type(s) and record the dominant species
x Trees x Shrubs Grasses Forbs Dominant species Black Walnut White Ash Hackberry and American Sycamore
INSTREAM FEATURES
Measured reach length 100 m Canopy Cover Estimated stream width _35__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area __350_m2
Estimated stream depth __15__m Proportion of Reach Represented by Stream High water mark __22___m Morphology Types Surface velocity (at thalweg) _035__msec x Riffle__30___ x Run__30___ x Pool__40___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___10____ m2 Density of LWD _______ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __30___ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present__________________________________________________________
WATER QUALITY Temperature __18___degC Water Odors x NormalNone Sewage Specific Conductance __-____ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ____-____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used _____NA____ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other___None_____ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach SubstrateType Characteristic
Composition in Sampling Reach
Bedrock 3 Detritus sticks wood coarse plant materials
2 Boulder gt256 mm (10rsquorsquo) 5
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 70 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 22 Sand 006-2 mm (gritty) 0 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiattrsquos Run LOCATION Rt 664 Bridge STATION HLR-3 STREAM CLASS LAT N 39 12934 (upstream) LONG W 078 05019 (upstream) LAT N 39 12958 (downstream) LONG W 078 04958 (downstream) FORM COMPILED BY Sean Robertson RIVER BASIN Potomac River INVESTIGATORS SRobertson BMillholland WBousquet MDahl JPangle
DATE 28 May 2008 TIME 1127 AM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality 2008
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature __14___degC showers (intermittent) x Trace Comments _______________________
_65 x cloud cover ___ ______________________________
x clearsunny __________________________________
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x (Significant springs andor seeps) x (Valley) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS
g
h
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence Some evidence x FieldPasture Industrial x Obvious sources Agricultural x Other Old Package Plant Downstream x Residential Bridge and Road Power line and Pump station Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left 70 field 30 shrubs and deciduous trees Bank=Soil and vegetation Right Maturing deciduous forest steep slope Bank=Shale Bedrock Indicate the dominant vegetation type(s) and record the dominant species
x Trees x Shrubs x Grasses Forbs Dominant species Sycamore and Red Oaks
INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width __51___m Fully open x Partly open Partly shaded Fully shaded Sampling reach area _510_m2
Estimated stream depth __135___m Proportion of Reach Represented by Stream High water mark __-___m Morphology Types Surface velocity (at thalweg) __20___msec x Riffle_40_ x Run_50_ x Pool 10 Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___0____ m2 Density of LWD ___-____ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation ___50____ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present Brown Algae
WATER QUALITY Temperature __17___degC Water Odors x NormalNone Sewage Specific Conductance ___-___ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ___-_____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used ____NA_______ Clear x Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other____None____ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 15 Detritus sticks wood coarse plant materials
0 Boulder gt256 mm (10rsquorsquo) 15
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 62 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 8 Sand 006-2 mm (gritty) 0 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Unnamed Tributary STATION HLR-4 STREAM CLASS LAT N 39 13035 (upstream) LONG W 078 05129 (upstream) LAT N 39 13000 (downstream) LONG W 078 05086 (downstream) FORM COMPILED BY Marie Dahl RIVER BASIN Potomac River INVESTIGATORS M Dahl HKepple SRobertson AFisher
DATE 31 May 2008 TIME 5 PM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality 2008
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) YES x NO
rain (steady rain) Air Temperature __22___degC showers (intermittent) Comments _______________________
_0__ cloud cover _0__ ______________________________
x clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area __-___ km2 mi2 Montane Significant springs andor seeps x Valley Marsh or swamp Montane amp Valley Mixture of origins Other ___________________
COMMENTS Snowden Bridge development (upstream) and sewer line
i
j
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence x FieldPasture Industrial Obvious sources Agricultural Other ____________ Construction upstream and adjacent x Residential Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left Forested with rocky banks field across silt fence and 2m tree strip Right Forested with some rocky banks and forest through the highway Indicate the dominant vegetation type(s) and record the dominant species
x Trees x Shrubs x Grasses Forbs Dominant species White Ash and Hackberry
INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width ___38__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area __380__m2
Estimated stream depth __12__cm Proportion of Reach Represented by Stream High water mark __2___m Morphology Types Surface velocity (at thalweg) __46___msec x Riffle__25___ x Run__70___ x Pool__5___ Channelized Yes x No Dam Present x Yes No
LARGE WOODY DEBRIS
LWD _______ m2 Density of LWD _______ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation ___NA____ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present___Algae___
WATER QUALITY Temperature __16___degC Water Odors x NormalNone Sewage Specific Conductance __-____ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ___-_____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used ____NA_____ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells x Other___Shale_______ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 0 Detritus sticks wood coarse plant materials
10 Boulder gt256 mm (10rsquorsquo) 5
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 45 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 30 Sand 006-2 mm (gritty) 0 Marl grey shell
fragments 0
Silt 0004-006 mm 20 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Lick Run STATION HLR-5 STREAM CLASS LAT N 39 127517 (upstream) LONG W 078 05685(upstream) LAT N 39 12784 (downstream) LONG W 078 05631 (downstream) FORM COMPILED BY B Millholland RIVER BASIN Potomac River INVESTIGATORS B Millholland JTovar SRobertson MDahl WBousquet JPangle
DATE 29 May 2008 TIME 1040 AM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project
k
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature __15___degC showers (intermittent) Comments _______________________
_5_ X cloud cover _0__ ______________________________
x clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane Significant springs andor seeps x Valley Marsh or swamp Montane amp Valley Mixture of origins Other ___________________
COMMENTS Lick Run appears to be intermittent and may be dry in Fall 2008
l
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence x FieldPasture Industrial Obvious sources Agricultural Other ____________ Adjacent logging stream bank scour Residential Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left 85 young deciduous forest 15 logged Bank=Soil vegetation and cobbles Right Young deciduous forest on 30 slope Bank= Soil vegetation and cobble Indicate the dominant vegetation type(s) and record the dominant species
x Trees Shrubs Grasses Forbs Dominant species Mixed Deciduous INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width __11__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area 110 m2
Estimated stream depth _009__m Proportion of Reach Represented by Stream High water mark __96___m Morphology Types Surface velocity (at thalweg) __01___msec x Riffle__30___ x Run__5___ x Pool__65___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___0____ m2 Density of LWD ___-____ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __70___ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present__________________________________________________________
WATER QUALITY Temperature __14___degC Water Odors x NormalNone Sewage Specific Conductance __-__ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ___-___ Water Surface Oils Slick Sheen Globs Flecks pH __-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used _____NA______ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other_____None_____
SEDIMENT AND SUBSTRATE
Odors Deposits Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other________________ x Other Slightly Salty Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 0 Detritus sticks wood coarse plant materials
5 Boulder gt256 mm (10rsquorsquo) 0
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 75 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 23 Sand 006-2 mm (gritty) 2 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION McCann Farm STATION HLR-6 STREAM CLASS LAT N 39 13076 (upstream) LONG W 078 06539 (upstream) LAT N 39 13063 (downstream) LONG W 078 06473 (downstream) FORM COMPILED BY MDahl RIVER BASIN Potomac River INVESTIGATORS MDahl JPangle SRobertson W Bousqet BMillholland JTovar H Kepple
DATE 30 May 2008 TIME 1050 AM (start)
PROJECT NAME
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) YES x NO
rain (steady rain) Air Temperature __18___degC showers (intermittent) Comments _______________________
_85_x cloud cover _10__ ______________________________
clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x (Significant springs andor seeps) x ( Valley) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS Multi-habitat sampling with D-frame nets and seine
m
n
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial x No evidence Some evidence x FieldPasture Industrial Obvious sources x Agricultural Other ____________ Minor stream bank scour Residential Local Watershed Erosion x None Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left 35 bank grasses scattered trees and some bushes Right High bank with grasses and scattered trees Indicate the dominant vegetation type(s) and record the dominant species
x Trees Shrubs Grasses Forbs Dominant species Black Walnut and Festuca elatior
INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width __2___m Fully open Partly open x Partly shaded Fully shaded Sampling reach area __200_m2
Estimated stream depth __03_m Proportion of Reach Represented by Stream High water mark __1___m Morphology Types Surface velocity (at thalweg) __166_msec x Riffle__0___ x Run__55___ x Pool__45___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___2____ m2 Density of LWD ___-____ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __20_____ x Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present ____Glyceria (grass)sp__________
WATER QUALITY Temperature __16___degC Water Odors x NormalNone Sewage Specific Conductance ___-___ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ____-____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used ______NA___ Clear x Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other__NONE__ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 0 Detritus sticks wood coarse plant materials
5 Boulder gt256 mm (10rsquorsquo) 0
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 27 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 38 Sand 006-2 mm (gritty) 5 Marl grey shell
fragments 0 (lt1)
Silt 0004-006 mm 25 Clay lt 0004 mm (slick) 5 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Appendix B
WATER QUALITY ANALYSIS THROUGH THE VIRGINIA
STREAM CONDITION INDEX (VSCI)
The Virginia Stream Condition Index (VSCI) relies on eight metrics to categorize stream health According to Burton and Gerritsen (2003 p 1-1) this index ldquohellip can be used as a primary indicator of ecosystem health and can identify impairment with respect to the reference (or natural) conditionrdquo These metrics are numeric measures of a biological communityrsquos species composition diversity ecological niches or tolerance to pollution
Table 13 on the next page describes each VSCI metric lists its formula and provides the best standard value (BSV) for that metric Based on a 100-point scale the BSV is the score that reflects optimum conditions for aquatic life ie the best water quality value possible (DEQ 2006b pp 8 33) The table also explains whether the score increases or decreases with environmental stress
As Table 13 indicates each of the eight VSCI metrics is calculated individually Each formula provides a unitless score based on a 100-point scale with 0 as the worst possible score and 100 as the best Any metric that provides a result above 100 is changed to 100 in order for the index to work properly After the eight metrics are calculated they are summed and averaged to provide a final VSCI score on a 100-point scale
Based on statistical analysis of Virginiarsquos streams and their BMI communities the DEQ (2006b p 8) currently uses the following tiers to interpret VSCI scores
VSCI Scores Aquatic Life Use
(ALI) Tiers
lt 42 severely stressed
42-55 moderately stressed
55-63 unknownfair (gray zone)
63-73 good
gt73 excellent
Section 2 of this report presents the results of calculations for the six BMI sampling sites the research team studied in the Hiatt-Lick Run subwatershed in 2008
o
p
1 Total taxa the number of distinct taxa in the sample measures the overall diversity of the macroinvertebrate assemblage The best standard value (BSV) is 189 Score = 100 x (X189) Total taxa decreases with stress 2 EPT taxa the sum of the distinct taxa in the orders Ephemeroptera (mayflies) Plecoptera (stoneflies) and Trichoptera (caddisflies) These orders are known to be sensitive to pollution The best standard value (BSV) is 117 Score = 100 x (X117) EPT taxa decreases with stress 3 Percent Ephemeroptera the percent abundance of individuals in the sample that belong to the order Ephemeroptera (mayflies) The best standard value (BSV) is 477 Score = 100 x (X477) Percent Ephemeroptera decreases with stress 4 Percent Plecoptera + Trichoptera less Hydropsychidae the percent abundance of individuals in the sample that belong to the orders Plecoptera (stoneflies) and Trichoptera (caddisflies) excluding the pollution-tolerant caddisfly family Hydropsychidae The best standard value (BSV) is 562 Score = 100 x (X562) Percent Plecoptera + Trichoptera less Hydropsychidae decreases with stress 5 Percent Scrapers the percent abundance of individuals in the sample whose primary functional mechanism for feeding is grazing on the substrate or periphyton (ie attached algae and associated material) The best standard value (BSV) is 467 Score = 100 x (X467) Percent scrapers decreases with stress 6 Percent Chironomidae the percent abundance of individuals in the sample that are Chironomidae (non-biting midge) larvae and pupae The best standard value (BSV) is 16 Score = 100 x [(100-X)(100-16)] Percent Chironomidae increases with stress 7 Percent Top Two Dominant the percent abundance of the two most frequent taxa present in the sample The best standard value (BSV) is 328 Score = 100 x [(100-X)(100-328)] The Percent Top 2 Dominant increases with stress 8 Hilsenhoff Biotic Index (HBI) an abundance-weighted average pollution tolerance of all the individuals within a sample keyed to the family level Tolerance ratings range from 1 (highly intolerant) to 10 (highly tolerant) The best standard value (BSV) is 29 Score = 100 x [(10-X)(10-29)] The Hilsenhoff Biotic Index (HBI) increases with stress ______________________________________________________________________________ Table 13 Description of Benthic Macroinvertebrate VSCI Metrics Includes responses (increases decreases) of each VSCI metric to degraded water quality conditions Adapted from Burton and Gerritsen (2003 pp 3-15 3-16 6-2) and DEQ (2008)
q
Appendix C
LABORATORY BENCH SHEETS
Shenandoah Universityrsquos Environmental Studies Department developed the Benthic Macroinvertebrate Laboratory Bench Sheet and VSCI Worksheet to record BMI specimen identifications and their VSCI classifications for each sample
This form is based in part on bench sheets developed by the EPA and Virginia DEQ for recording specimen identifications Additional columns on Shenandoah Universityrsquos forms for relative numbers and VSCI classifications make the calculation and verification of VSCI scores easier
Note the column for taxonomistrsquos initials Specimens were identified by students in Shenandoah Universityrsquos research group and then verified or where necessary changed by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality This external review helps assure the validity of the study Originals of these bench sheets with handwritten taxonomistsrsquo initials will be kept on file at Shenandoah Universityrsquos Environmental Studies Department for five years after the completion of this study
r
s
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-1 STATION NAME Mouth LAT N 39deg 12749rsquo (upstream) LONG W 078deg 04209rsquo (upstream) LAT N 39deg 12729rsquo (downstream) LONG W 078deg 04148rsquo (downstream) FORM COMPILED BY Jeremy Tovar W S Bousquet RIVER BASIN Potomac River INVESTIGATORS Marie Dahl Brandon Millholand J W Pangle Sean Robertson Jeremy Tovar Woodward Bousquet Ben Bousquet
Collection date 21 May 2008 Sheet completion date 12 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Hirudinea 3 33 JT BVW PR 8 SP Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Coenagrionidae 2 22 BVW PR 9 CR Ephemeroptera (mayflies) Caenidae 1 11 JT BVW CG 6 SP Ephemerllidae 4 44 JT BVW CG 2 CR Heptageniidae 5 55 JT BVW SC 3 CG Isonychiidae 1 11 BVW CF 3 CG Plecoptera (stoneflies) Perlidae 14 154 JT BVW PR 2 CR Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Corydalidae 1 11 JT BVW PR 5 CR Coleoptera (beetles) Elmidae 24 264 I JT BVW SC 4 CG Dytiscidae 4 44 JT BVW PR 6 GN Psephenidae 3 33 JT BVW SC 4 CG Diptera (true flies) Tipulidae 3 33 JT BVW SH 4 BU Chironomidae 26 286 JT BVW CG 6 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
t
Other Total Individuals 91 1001 Total Taxa 13
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
u
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-1 STATION NAME Mouth LAT N 39deg 12749rsquo (upstream) LONG W 078deg 04209rsquo (upstream) LAT N 39deg 12729rsquo (downstream) LONG W 078deg 04148rsquo (downstream) FORM COMPILED BY Jeremy Tovar W S Bousquet RIVER BASIN Potomac River INVESTIGATORS Brandon Millholand JW Pangle Jeremy Tovar Heather Kepple
Collection date 10 October 2008 Sheet completion date 12 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Oligochaeta 3 35 JT BVW CG 8 BU Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Coenagrionidae 2 23 JT BVW PR 9 CR Ephemeroptera (mayflies) Heptageniidae 12 140 JT BVW SC 3 CG Isonychiidae 2 23 BVW CF 3 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Hydropsychidae 16 186 JT BVW CF 6 CG Philopotamidae 31 360 JT BVW CF 2 CG Hemiptera (true bugs) (Veliidae) (5) - JT BVW ( ) Megaloptera (dobsonflies etc) Corydalidae 10 116 JT BVW PR 5 CR Coleoptera (beetles) Dytiscidae 1 12 JT BVW PR 6 GN Psephenidae 7 81 JT BVW SC 4 CG Diptera (true flies) Tipulidae 2 23 BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
v
Other Total Individuals 86
(91) 999
Total Taxa 10 (11)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009 Veliidae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
w
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-2 STATION NAME Package Plant LAT N 39deg 12958rsquo (upstream) LONG W 078deg 04900 (upstream) LAT N 39deg 12958rsquo (downstream) LONG W 078deg 04822 (downstream) FORM COMPILED BY JW Pangle W Bousquet RIVER BASIN Potomac River INVESTIGATORS JW Pangle S Robertson J Tovar M Dahl B Millholland W Bousquet
Collection date 29 May 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Plecoptera (stoneflies) Perlidae 11 136 I JP BVW PR 1 CR Trichoptera (caddisflies) Hydropsychidae 10 123 I JP BVW CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 14 173 I JP BVW SC 4 CG Diptera (true flies) Chironomidae 32 395 I JP BVW CG 6 BU Simuliidae 14 173 I JP BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
x
Other Total Individuals 81 1000 Total Taxa 5
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-2 STATION NAME Package Plant LAT N 39deg 12958rsquo (upstream) LONG W 078deg 04900 (upstream) LAT N 39deg 12958rsquo (downstream) LONG W 078deg 04822 (downstream) FORM COMPILED BY JW Pangle W Bousquet RIVER BASIN Potomac River INVESTIGATORS JW Pangle Brandon Millholland Jeremy Tovar Heather Kepple
Collection date 10 October 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Heptageniidae 40 392 I WBJP SC 3 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Hydropsychidae 7 69 I JP WB BVW CF 6 CG Philopotamidae 28 275 I JP WB BVW CF 2 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Corydalidae 6 59 I JP WB BVW PR 5 CR Coleoptera (beetles) Psephenidae 7 69 I JP WB BVW SC 4 CG Elmidae 8 78 I JP WB BVW SC 4 CG Diptera (true flies) Tipulidae 6 59 I JP WB BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
y
z
Other Total Individuals 102 1001 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-3 STATION NAME Rt 664 Bridge LAT N 39deg 12934rsquo (upstream) LONG W 078deg 05019rsquo (upstream) LAT N 39deg 12951rsquo (downstream) LONG W 078deg 04958rsquo (downstream) FORM COMPILED BY S Robertson W Bousquet RIVER BASIN Potomac River INVESTIGATORS SRobertson BMillholland WBousquet MDahl JPangle
Collection date 28 May 2008 Sheet completion date 11 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Leptophlebiidae 1 15 I SGR BVW CG 2 CR Baetidae 1 15 I BVW CG 5 CG Plecoptera (stoneflies) Perlidae 3 46 I SGR BVW PR 1 CR Nemouridae 1 15 I BVW SH 3 CR Trichoptera (caddisflies) Hydropsychidae 5 77 I SGR BVW CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 27 415 I SGR BVW SC 4 CG Psephenidae 2 31 A SGR BVW SC 4 CG Diptera (true flies) Simuliidae 5 77 I SGR BVW CF 6 CG Chironomidae 20 308 I SGR BVW CG 6 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
aa
bb
Other Total Individuals 65 999 Total Taxa 9
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-3 STATION NAME Rt 664 Bridge LAT N 39deg 12934rsquo (upstream) LONG W 078deg 05019rsquo (upstream) LAT N 39deg 12951rsquo (downstream) LONG W 078deg 04958rsquo (downstream) FORM COMPILED BY S Robertson RIVER BASIN Potomac River INVESTIGATORS S Robertson M Dahl A Fisher H Kepple
Collection date 10 Oct 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Heptageniidae 2 20 I SGR SC 3 CG Isonychiidae 2 20 I BVW CF 2 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Philopotamidae 36 367 I SGR CF 2 CG Hydropsychidae 47 480 I SGR CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 2 20 A SGR SC 4 CG Psephenidae 8 82 A SGR SC 4 CG Diptera (true flies) Tabanidae 1 10 I SGR PR 7 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
cc
dd
Other Total Individuals 98 999 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-4 STATION NAME Unnamed Tributary LAT (upstream) N 39deg 13035rsquo LONG (upstream) W 078deg 05129rsquo LAT (downstream) N 39deg 13000rsquo LONG (downstream) W 078deg 5086rsquo FORM COMPILED BY A Fisher M Dahl rev W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson A Fisher H Kepple
Collection date 31 May 2008 Sheet completion date rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Flatworms (Turbellaria) Worms (Oligochaeta) Naididae 1 14 - BVW CG 8 BU Leeches (Hirudinea) Snails (Gastropoda) Clams (Bivalvia) Isopods (Isopoda) Asellidae 3 42 A AF BVW CG 8 SP Scuds (Amphipoda) Crayfish Shrimp (Decapoda) Water Mites (Hydracarina) Dragonflies (Odonata Anisoptera) Damselflies (Odonata Zygoptera) Mayflies (Ephemeroptera) Stoneflies (Plecoptera) Perlidae 2 28 I AF BVW PR 1 CR Caddisflies (Trichoptera) True Bugs (Hemiptera) Corixidae 1 14 I AF BVW MP 9 DV Dobsonflies (Megaloptera) Beetles (Coleoptera) Dytiscidae 1 14 I AF BVW PR 6 GN True Flies (Diptera) Chironomidae 61 847 I AF BVW CG 6 BU Simuliidae 3 42 I AF BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
ee
ff
Other Total Individuals 72 1001 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-4 STATION NAME Unnamed Tributary LAT (upstream) N 39deg 13035rsquo LONG (upstream) W 078deg 05129rsquo LAT (downstream) N 39deg 13000rsquo LONG (downstream) W 078deg 5086rsquo FORM COMPILED BY Andrew G M Fisher W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson A Fisher H Kepple
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Flatworms (Turbellaria) Worms (Oligochaeta) Lumbriculidae 2 32 A AF BVW CG 8 BU Leeches (Hirudinea) Snails (Gastropoda) Lymnaeidae 1 16 A BVW CG 9 GN Clams (Bivalvia) Isopods (Isopoda) Scuds (Amphipoda) Crayfish Shrimp (Decapoda) Cambaridae 1 16 A AF BVW GN 5 GN Water Mites (Hydracarina) Dragonflies (Odonata Anisoptera) Damselflies (Odonata Zygoptera) Calopterygidae 4 63 I BVW PR 7 CL Mayflies (Ephemeroptera) Tricorythidae 1 16 I BVW CG 5 SP Stoneflies (Plecoptera) Caddisflies (Trichoptera) Phryganeidae 4 63 I AF BVW SH 4 CR Hydropsychidae 6 95 I AF BVW CF 6 CG I True Bugs (Hemiptera) Corixidae 8 127 I AF BVW MP 9 DV (Veliidae ) (7) - I AF BVW ( ) Dobsonflies (Megaloptera) Sialidae 3 48 I AF BVW PR 7 SP Corydalidae 2 32 I AF BVW PR 5 CR Beetles (Coleoptera) Haliplidae 1 16 A AF BVW MP 8 GN Dysticidae 7 111 I AF BVW PR 6 GN Hydrophilidae 1 16 A AF BVW PR 8 GN Elmidae 3 48 I BVW SC 4 CG True Flies (Diptera) Chironomidae 8 127 I AF BVW CG 6 BU Simuliidae 7 111 I AF BVW CF 6 CG Tipulidae 4 63 I AF BVW SH 4 GU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
gg
hh
Other Total Individuals 63
(70) 1000
Total Taxa 17 (18)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Veliidae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-5 STATION NAME Lick Run LAT N 39deg 12752rsquo (upstream) LONG W 078deg 05685rsquo (upstream) LAT N 39deg 12784rsquo (downstream) LONG W 078deg 05631rsquo (downstream) FORM COMPILED BY Brandon Millholland W Bousquet RIVER BASIN Potomac River INVESTIGATORS J Pangle S Robertson W Bousquet JTovar M Dahl
Collection date 29 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Gomphidae 1 15 I BVW PR 3 BU Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Leptophlebiidae 11 167 A LBM BVW CG 2 CR Plecoptera (stoneflies) Perlidae 10 152 A LBM BVW PR 1 CR Perlodiae 1 15 A LBM BVW PR 2 CR Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 5 76 A LBM BVW SC 4 CG Elmidae 2 30 I LBM BVW SC 4 CG Diptera (true flies) (Culicidae) (3) - P LMB BVW ( ) Chironomidae 23 348 I LBM BVW CG 6 BU Simuliidae 13 197 I LBM BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
ii
jj
Other Total Individuals 66
(69) 1000
Total Taxa 7 (8)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Culicidae (Diptera) pupae not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl J Pangle S Robertson W Bousquet B Millholland J Tovar H Kepple
Collection date 30 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 1 12 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Asellidae 114 965 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Plecoptera (stoneflies) Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 2 23 A MRD BVW SC 4 CG Diptera (true flies)
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
kk
ll
Other Total Individuals 117 1000 Total Taxa 3
Notes Subsample taken 29 October 2009 All taxonomic determinations by MRD were confirmed by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson K McCann A Fisher H Kepple A Barrett
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 2 17 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Sphaeriidae 2 17 I MRD BVW CF 8 BU Isopoda (isopods) Asellidae 99 853 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Calopterygidae 2 17 A MRD BVW PR 7 CL Ephemeroptera (mayflies) Leptophlebiidae 2 17 I MRD BVW CG 2 CR Heptageniidae 4 34 A MRD BVW SC 3 CG Plecoptera (stoneflies) Perlidae 1 09 A MRD BVW PR 1 CR Trichoptera (caddisflies) Hemiptera (true bugs) (Gerridae) (1) - A MRD BVW ( ) Corixidae 1 09 A MRD BVW PR 5 CR Megaloptera (dobsonflies etc) Coleoptera (beetles) Dryopidae 1 09 A MRD BVW SC 5 CG Diptera (true flies) Tipulidae 2 17 I BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
mm
nn
Other Total Individuals 116
(117) 999
Total Taxa 10 (11)
Notes Subsample taken 6 November 2008 Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009 Gerridae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
- Disclaimer ii
- Title Page iii
- Acknowledgements v
- Table of Contents vii
- SECTION 2 RESULTS DATA TABLES 7
- OVERVIEW AND FINDINGS
- Introduction
- RESULTS DATA TABLES
- REFERENCES
- FIELD DATA SHEETS
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- SubstrateType
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- WATER QUALITY ANALYSIS
- THROUGH THE VIRGINIA
- STREAM CONDITION INDEX (VSCI)
- LABORATORY BENCH SHEETS
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
6
Section 2
RESULTS DATA TABLES
Site HLR-1 Mouth
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 13 189 6872 EPT taxa 5 117 4273 Ephemeroptera 121 477 2544 Plec +Tric less Hydropsych 154 562 2745 Scrapers 352 467 7536 Chironomidae 286 16 7267 Top 2 Dominant 550 328 6708 Hilsenhoff Biotic Index (HBI) 45 29 775VSCI Score 571
According to the DEQrsquos water quality standards aquatic life at this site is fair (gray zone) See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 2 VSCI Scores for Mouth (HLR-1) Site in Hiatt-Lick Run Sampled on 21 May 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 10 189 5292 EPT taxa 4 117 3423 Ephemeroptera 163 477 3424 Plec +Tric less Hydropsych 360 562 6415 Scrapers 221 467 4736 Chironomidae 00 16 10007 Top 2 Dominant 546 328 6768 Hilsenhoff Biotic Index (HBI) 39 29 859VSCI Score 608
According to the DEQrsquos water quality standards aquatic life at this site is fair (gray zone) See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 3 VSCI Scores for Mouth (HLR-1) Site in Hiatt-Lick Run Sampled 10 October 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
7
Site HLR-2 Package Plant
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 5 189 2642 EPT taxa 2 117 1713 Ephemeroptera 00 477 004 Plec +Tric less Hydropsych 110 562 1965 Scrapers 173 467 3706 Chironomidae 395 16 6157 Top 2 Dominant 568 328 6438 Hilsenhoff Biotic Index (HBI) 50 29 704VSCI Score 370
According to the DEQrsquos water quality standards aquatic life at this site is severely stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 4 VSCI Scores for Package Plant (HLR-2) Site in Hiatt-Lick Run Sampled 29 May 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 7 189 3702 EPT taxa 34 117 2563 Ephemeroptera 392 477 8214 Plec +Tric less Hydropsych 275 562 4895 Scrapers 539 467 10006 Chironomidae 00 16 10007 Top 2 Dominant 667 328 4968 Hilsenhoff Biotic Index (HBI) 32 29 958VSCI Score 674
According to the DEQrsquos water quality standards aquatic life at this site is good See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 5 VSCI Scores for Package Plant (HLR-2) Site in Hiatt-Lick Run Sampled 10 October 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
8
Site HLR-3 Route 664 Bridge
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 9 189 4762 EPT taxa 5 117 4273 Ephemeroptera 30 477 634 Plec +Tric less Hydropsych 61 562 1095 Scrapers 446 467 9556 Chironomidae 308 16 7037 Top 2 Dominant 723 328 4128 Hilsenhoff Biotic Index (HBI) 48 29 732VSCI Score 485
According to the DEQrsquos water quality standards aquatic life at this site is moderately stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 6 VSCI Scores for Route 664 Bridge (HLR-3) Site in Hiatt-Lick Run Sampled 28 May 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 7 189 3702 EPT taxa 4 117 3423 Ephemeroptera 40 477 844 Plec +Tric less Hydropsych 367 562 6535 Scrapers 122 467 2616 Chironomidae 00 16 10007 Top 2 Dominant 847 328 2288 Hilsenhoff Biotic Index (HBI) 42 29 817VSCI Score 469
According to the DEQrsquos water quality standards aquatic life at this site is moderately stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 7 VSCI Scores for Route 664 Bridge (HLR-3) Site in Hiatt-Lick Run Sampled 10 October 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
9
Site HLR-4 Unnamed Tributary
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 7 189 3702 EPT taxa 1 117 853 Ephemeroptera 00 477 004 Plec +Tric less Hydropsych 28 562 495 Scrapers 00 467 006 Chironomidae 847 16 1557 Top 2 Dominant 889 328 1658 Hilsenhoff Biotic Index (HBI) 60 29 563VSCI Score 173
According to the DEQrsquos water quality standards aquatic life at this site is severely stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 8 VSCI Scores for Unnamed Tributary (HLR-4) Site in Hiatt-Lick Run Sampled 31 May 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 17 189 8992 EPT taxa 3 117 2563 Ephemeroptera 16 477 344 Plec +Tric less Hydropsych 63 562 1125 Scrapers 48 467 1036 Chironomidae 127 16 8877 Top 2 Dominant 254 328 10008 Hilsenhoff Biotic Index (HBI) 63 29 521VSCI Score 476
According to the DEQrsquos water quality standards aquatic life at this site is moderately stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 9 VSCI Scores for Unnamed Tributary (HLR-4) Site in Hiatt-Lick Run Sampled 10 October 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
10
Site HLR-5 Lick Run
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 7 189 3702 EPT taxa 3 117 2563 Ephemeroptera 167 477 3504 Plec +Tric less Hydropsych 167 562 2975 Scrapers 106 467 2276 Chironomidae 348 16 6637 Top 2 Dominant 545 328 6778 Hilsenhoff Biotic Index (HBI) 43 29 803VSCI Score 455
According to the DEQrsquos water quality standards aquatic life at this site is moderately stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 10 VSCI Scores for Lick Run (HLR-5) Site in Hiatt-Lick Run Sampled 29 May 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
Note
Water conditions at the Lick Run (HLR-5) site were too low in October 2008 to permit a sample to be taken
11
Site HLR-6 McCann Farm
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 3 189 1592 EPT taxa 0 117 003 Ephemeroptera 00 477 004 Plec +Tric less Hydropsych 00 562 005 Scrapers 23 467 496 Chironomidae 00 16 10007 Top 2 Dominant 988 328 188 Hilsenhoff Biotic Index (HBI) 79 29 296VSCI Score 190
According to the DEQrsquos water quality standards aquatic life at this site is severely stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 11 VSCI Scores for McCann Farm (HLR-6) Site in Hiatt-Lick Run Sampled 30 May 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 10 189 5292 EPT taxa 3 117 2563 Ephemeroptera 51 477 1074 Plec +Tric less Hydropsych 09 562 165 Scrapers 43 467 926 Chironomidae 00 16 10007 Top 2 Dominant 887 328 1688 Hilsenhoff Biotic Index (HBI) 75 29 352VSCI Score 315
According to the DEQrsquos water quality standards aquatic life at this site is severely stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 12 VSCI Scores for McCann Farm (HLR-6) Site in Hiatt-Lick Run Sampled 10 October 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
12
REFERENCES
Barbour Michael T Jeroen Gerritsen Blaine D Snyder and James B Stribling 1999 Rapid bioassessment protocols for use in streams and wadeable rivers periphyton benthic macroinvertebrates and fish Second edition EPA 841-B-99-002 Washington DC US Environmental Protection Agency Office of Water Available online at httpwwwepagovOWOWmonitoringbioasspshtml Burton June and Jeroen Gerritsen 2003 A stream condition index for Virginia non-coastal streams Prepared for the US EPA Office of Science and Technology US EPA Region 3 Environmental Services Division and Virginia Department of Environmental Quality Owings Mills Maryland Tetra Tech Inc Available online at httpwwwdeqvirginiagovwatermonitoringpdf vastrmconpdf Hessler Rachel A Andrea R Martin Heidi D Ruggeri William B Seymour Jr and Woodward S Bousquet 2006 Water quality assessment using benthic macroinvertebrates A study and compilation Winchester Virginia Environmental Studies Program Shenandoah University Occasional publication no 10 Igoe Robert 2006 DEQ identifies nine impaired streams in Clarke Frederick The Winchester Star 15 July B1 Jackson Charlie 2005 Survey targets Opequon cleanup The Winchester Star 19 August B1 Johnston Elizabeth A and Woodward S Bousquet 2003 What happens when it rains A study of water quality in Abrams Creek and Town Run Winchester and Frederick County Virginia Winchester Virginia Environmental Studies Program Shenandoah University Occasional publication no7 Merritt Richard W and Kenneth W Cummins 1996 An introduction to the aquatic insects of North America Dubuque Iowa KendallHunt Publishing Company Opequon Creek IP Steering Committee Virginia Tech Department of Biological Systems Engineering and Center for TMDL and Watershed Studies University of Virginia Institute for Environmental Negotiation and West Virginia University Division of Resource Management 2006 Opequon Creek watershed TMDL implementation plan Blacksburg Virginia Virginia Tech Department of Biological Systems Engineering Available online at httpwwwtmdlbsevteduoutreachC82 Pennak Robert W 1953 Fresh-water invertebrates of the United States New York New York The Ronald Press Company Rosenburg David M and Vincent H Resh 1993 Freshwater biomonitoring and benthic macroinvertebrates New York New York Chapman amp Hall Shenandoah University Public Relations Office 2002 Data collected by SU and its partners helps identify impaired water Winchester Virginia Available online at httpwwwsuedutemp_ newscfmurlnum=32 Thorp James H and Alan P Covich 1991 Ecology and classification of North American freshwater invertebrates San Diego California Academic Press Inc
13
US Environmental Protection Agency 1997 Volunteer stream monitoring a methods manual EPA 841-B-97-003 Washington DC US Environmental Protection Agency Office of Water Van Meter Val 2003 Opequon Creek pollution limits to be established The Winchester Star 4 April B1 Virginia Department of Environmental Quality 2006a Virginia 305(b)303(d) water quality integrated report to Congress and the EPA Administrator for the period January 1 2000 to December 31 2004 Richmond Virginia Virginia Department of Environmental Quality Available online at httpwwwdeqvirginiagovwqair2006html __________ 2006b Using probabilistic data to validate the non-coastal Virginia Stream Condition Index (draft) VDEQ technical bulletin WQA2006-001 Virginia Department of Environmental Quality Available online at httpwwwloudounwatershedorgstream_assessmentreference_documents VA_DEQ_SCI_Validationpdf __________ 2008 Biological monitoring program quality assurance project plan for wadeable streams and rivers Richmond Virginia Virginia Department of Environmental Quality Update available online at httpwwwdeqstatevausbiomonitoringdocumentsBioMonQAPP_13Aug2008pdf Virginia Tech Aquatic Entomology Laboratory 2005 Taxa dictionary [Excel file] Blacksburg Virginia Virginia Tech Department of Biological Systems Engineering 2003a Opequon watershed TMDLs for benthic impairments Abrams Creek and Lower Opequon Creek Frederick and Clarke Counties Virginia Prepared for the Virginia Department of Environmental Quality and Virginia Department of Conservation and Recreation Blacksburg Virginia Available online at httpwwwdeqstate vaustmdlapptmdlsshenrvrabropebcpdf Voshell J Reese Jr 2002 A guide to common freshwater invertebrates of North America Blacksburg Virginia The McDonald amp Woodward Publishing Company
14
APPENDICES
15
16
a
Appendix A
FIELD DATA SHEETS
This appendix presents a Site Characterization Field Data Sheet for each of the six sampling reaches established on Hiatt Run and Lick Run for this study They were filled out during the sampling runs made in May 2008
These forms were adapted by Shenandoah University from forms used by the EPA and Virginia DEQ for recording characteristics of sampling sites
b
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Mouth STATION _HLR-1_____ STREAM CLASS LAT N 39 12749 (upstream) LONG W 078 04209(upstream) LAT N 39 12729 (downstream) LONG W 078 04148 (downstream) FORM COMPILED BY Jeremy Tovar RIVER BASIN Potomac River INVESTIGATORS JPangle B Millholland MDahl SRobertson W Bousquet JTovar BBousquet
DATE May 21 2008_________ TIME_250 PM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature __24___degC showers (intermittent) Comments _______________________
___ cloud cover _0_ ______________________________
X clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x ( Significant springs andor seeps) x (Valley ) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS
c
d
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence x FieldPasture Industrial Obvious sources Agricultural Other ____________ Stream scour steep slope erosion x Residential Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left Field and young thin deciduous forest 100 Bank=Grass cobble and shrub Right 90 maturing deciduous forest 45 shale slope Bank=Bedrock soil and herbs Indicate the dominant vegetation type(s) and record the dominant species
x Trees Shrubs x (Grasses) Forbs Dominant species Oak species and mix deciduous
INSTREAM FEATURES
Measured reach length ___100__m Canopy Cover Estimated stream width _35__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area __350_m2
Estimated stream depth _008_m Proportion of Reach Represented by Stream High water mark _15_m Morphology Types Surface velocity (at thalweg) _05__msec x Riffle__75___ x Run__20___ x Pool__5___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___00__ m2 Density of LWD _-_ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __15_____ x Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present Algae
WATER QUALITY Temperature __19___degC Water Odors x NormalNone Sewage Specific Conductance - Petroleum Chemical Fishy Other___________________ Dissolved Oxygen - Water Surface Oils Slick Sheen Globs Flecks pH __-_ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used _____NA________ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic x None Relict shells Other______None_____ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 8 Detritus sticks wood coarse plant materials
0 Boulder gt256 mm (10rsquorsquo) 0
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 51 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 40 Sand 006-2 mm (gritty) 1 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University
SITE CHARACTERIZATION FIELD DATA SHEET FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Package Plant STATION HLR-2 STREAM CLASS LAT N 39 129580 (upstream) LONG W 078 04900 (upstream) LAT N 39 129581 (downstream) LONG W 078 04822 (downstream) FORM COMPILED BY JW Pangle RIVER BASIN Potomac River INVESTIGATORS JPangle JTovar MDahl B Millholland SRobertson W Bousquet
DATE__19 May 2008_ TIME__1240__ PM
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature _____degC showers (intermittent) Comments __Sunny Day_____________
_5__ cloud cover _0__ ______________________________
x clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x (Significant springs andor seeps) x (Valley) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS
e
f
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence FieldPasture Industrial Obvious sources Agricultural Other ____________ __Upstream Construction and Stream bank Scour Residential __________________________________ Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left Deciduous forest with thick herb and shrub layers bank= vegetation and rocks Right Deciduous forest with thick herb and shrub layers bank=vegetation soil and rocks Indicate the dominant vegetation type(s) and record the dominant species
x Trees x Shrubs Grasses Forbs Dominant species Black Walnut White Ash Hackberry and American Sycamore
INSTREAM FEATURES
Measured reach length 100 m Canopy Cover Estimated stream width _35__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area __350_m2
Estimated stream depth __15__m Proportion of Reach Represented by Stream High water mark __22___m Morphology Types Surface velocity (at thalweg) _035__msec x Riffle__30___ x Run__30___ x Pool__40___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___10____ m2 Density of LWD _______ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __30___ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present__________________________________________________________
WATER QUALITY Temperature __18___degC Water Odors x NormalNone Sewage Specific Conductance __-____ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ____-____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used _____NA____ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other___None_____ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach SubstrateType Characteristic
Composition in Sampling Reach
Bedrock 3 Detritus sticks wood coarse plant materials
2 Boulder gt256 mm (10rsquorsquo) 5
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 70 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 22 Sand 006-2 mm (gritty) 0 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiattrsquos Run LOCATION Rt 664 Bridge STATION HLR-3 STREAM CLASS LAT N 39 12934 (upstream) LONG W 078 05019 (upstream) LAT N 39 12958 (downstream) LONG W 078 04958 (downstream) FORM COMPILED BY Sean Robertson RIVER BASIN Potomac River INVESTIGATORS SRobertson BMillholland WBousquet MDahl JPangle
DATE 28 May 2008 TIME 1127 AM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality 2008
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature __14___degC showers (intermittent) x Trace Comments _______________________
_65 x cloud cover ___ ______________________________
x clearsunny __________________________________
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x (Significant springs andor seeps) x (Valley) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS
g
h
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence Some evidence x FieldPasture Industrial x Obvious sources Agricultural x Other Old Package Plant Downstream x Residential Bridge and Road Power line and Pump station Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left 70 field 30 shrubs and deciduous trees Bank=Soil and vegetation Right Maturing deciduous forest steep slope Bank=Shale Bedrock Indicate the dominant vegetation type(s) and record the dominant species
x Trees x Shrubs x Grasses Forbs Dominant species Sycamore and Red Oaks
INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width __51___m Fully open x Partly open Partly shaded Fully shaded Sampling reach area _510_m2
Estimated stream depth __135___m Proportion of Reach Represented by Stream High water mark __-___m Morphology Types Surface velocity (at thalweg) __20___msec x Riffle_40_ x Run_50_ x Pool 10 Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___0____ m2 Density of LWD ___-____ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation ___50____ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present Brown Algae
WATER QUALITY Temperature __17___degC Water Odors x NormalNone Sewage Specific Conductance ___-___ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ___-_____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used ____NA_______ Clear x Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other____None____ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 15 Detritus sticks wood coarse plant materials
0 Boulder gt256 mm (10rsquorsquo) 15
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 62 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 8 Sand 006-2 mm (gritty) 0 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Unnamed Tributary STATION HLR-4 STREAM CLASS LAT N 39 13035 (upstream) LONG W 078 05129 (upstream) LAT N 39 13000 (downstream) LONG W 078 05086 (downstream) FORM COMPILED BY Marie Dahl RIVER BASIN Potomac River INVESTIGATORS M Dahl HKepple SRobertson AFisher
DATE 31 May 2008 TIME 5 PM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality 2008
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) YES x NO
rain (steady rain) Air Temperature __22___degC showers (intermittent) Comments _______________________
_0__ cloud cover _0__ ______________________________
x clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area __-___ km2 mi2 Montane Significant springs andor seeps x Valley Marsh or swamp Montane amp Valley Mixture of origins Other ___________________
COMMENTS Snowden Bridge development (upstream) and sewer line
i
j
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence x FieldPasture Industrial Obvious sources Agricultural Other ____________ Construction upstream and adjacent x Residential Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left Forested with rocky banks field across silt fence and 2m tree strip Right Forested with some rocky banks and forest through the highway Indicate the dominant vegetation type(s) and record the dominant species
x Trees x Shrubs x Grasses Forbs Dominant species White Ash and Hackberry
INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width ___38__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area __380__m2
Estimated stream depth __12__cm Proportion of Reach Represented by Stream High water mark __2___m Morphology Types Surface velocity (at thalweg) __46___msec x Riffle__25___ x Run__70___ x Pool__5___ Channelized Yes x No Dam Present x Yes No
LARGE WOODY DEBRIS
LWD _______ m2 Density of LWD _______ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation ___NA____ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present___Algae___
WATER QUALITY Temperature __16___degC Water Odors x NormalNone Sewage Specific Conductance __-____ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ___-_____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used ____NA_____ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells x Other___Shale_______ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 0 Detritus sticks wood coarse plant materials
10 Boulder gt256 mm (10rsquorsquo) 5
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 45 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 30 Sand 006-2 mm (gritty) 0 Marl grey shell
fragments 0
Silt 0004-006 mm 20 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Lick Run STATION HLR-5 STREAM CLASS LAT N 39 127517 (upstream) LONG W 078 05685(upstream) LAT N 39 12784 (downstream) LONG W 078 05631 (downstream) FORM COMPILED BY B Millholland RIVER BASIN Potomac River INVESTIGATORS B Millholland JTovar SRobertson MDahl WBousquet JPangle
DATE 29 May 2008 TIME 1040 AM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project
k
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature __15___degC showers (intermittent) Comments _______________________
_5_ X cloud cover _0__ ______________________________
x clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane Significant springs andor seeps x Valley Marsh or swamp Montane amp Valley Mixture of origins Other ___________________
COMMENTS Lick Run appears to be intermittent and may be dry in Fall 2008
l
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence x FieldPasture Industrial Obvious sources Agricultural Other ____________ Adjacent logging stream bank scour Residential Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left 85 young deciduous forest 15 logged Bank=Soil vegetation and cobbles Right Young deciduous forest on 30 slope Bank= Soil vegetation and cobble Indicate the dominant vegetation type(s) and record the dominant species
x Trees Shrubs Grasses Forbs Dominant species Mixed Deciduous INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width __11__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area 110 m2
Estimated stream depth _009__m Proportion of Reach Represented by Stream High water mark __96___m Morphology Types Surface velocity (at thalweg) __01___msec x Riffle__30___ x Run__5___ x Pool__65___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___0____ m2 Density of LWD ___-____ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __70___ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present__________________________________________________________
WATER QUALITY Temperature __14___degC Water Odors x NormalNone Sewage Specific Conductance __-__ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ___-___ Water Surface Oils Slick Sheen Globs Flecks pH __-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used _____NA______ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other_____None_____
SEDIMENT AND SUBSTRATE
Odors Deposits Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other________________ x Other Slightly Salty Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 0 Detritus sticks wood coarse plant materials
5 Boulder gt256 mm (10rsquorsquo) 0
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 75 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 23 Sand 006-2 mm (gritty) 2 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION McCann Farm STATION HLR-6 STREAM CLASS LAT N 39 13076 (upstream) LONG W 078 06539 (upstream) LAT N 39 13063 (downstream) LONG W 078 06473 (downstream) FORM COMPILED BY MDahl RIVER BASIN Potomac River INVESTIGATORS MDahl JPangle SRobertson W Bousqet BMillholland JTovar H Kepple
DATE 30 May 2008 TIME 1050 AM (start)
PROJECT NAME
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) YES x NO
rain (steady rain) Air Temperature __18___degC showers (intermittent) Comments _______________________
_85_x cloud cover _10__ ______________________________
clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x (Significant springs andor seeps) x ( Valley) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS Multi-habitat sampling with D-frame nets and seine
m
n
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial x No evidence Some evidence x FieldPasture Industrial Obvious sources x Agricultural Other ____________ Minor stream bank scour Residential Local Watershed Erosion x None Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left 35 bank grasses scattered trees and some bushes Right High bank with grasses and scattered trees Indicate the dominant vegetation type(s) and record the dominant species
x Trees Shrubs Grasses Forbs Dominant species Black Walnut and Festuca elatior
INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width __2___m Fully open Partly open x Partly shaded Fully shaded Sampling reach area __200_m2
Estimated stream depth __03_m Proportion of Reach Represented by Stream High water mark __1___m Morphology Types Surface velocity (at thalweg) __166_msec x Riffle__0___ x Run__55___ x Pool__45___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___2____ m2 Density of LWD ___-____ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __20_____ x Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present ____Glyceria (grass)sp__________
WATER QUALITY Temperature __16___degC Water Odors x NormalNone Sewage Specific Conductance ___-___ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ____-____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used ______NA___ Clear x Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other__NONE__ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 0 Detritus sticks wood coarse plant materials
5 Boulder gt256 mm (10rsquorsquo) 0
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 27 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 38 Sand 006-2 mm (gritty) 5 Marl grey shell
fragments 0 (lt1)
Silt 0004-006 mm 25 Clay lt 0004 mm (slick) 5 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Appendix B
WATER QUALITY ANALYSIS THROUGH THE VIRGINIA
STREAM CONDITION INDEX (VSCI)
The Virginia Stream Condition Index (VSCI) relies on eight metrics to categorize stream health According to Burton and Gerritsen (2003 p 1-1) this index ldquohellip can be used as a primary indicator of ecosystem health and can identify impairment with respect to the reference (or natural) conditionrdquo These metrics are numeric measures of a biological communityrsquos species composition diversity ecological niches or tolerance to pollution
Table 13 on the next page describes each VSCI metric lists its formula and provides the best standard value (BSV) for that metric Based on a 100-point scale the BSV is the score that reflects optimum conditions for aquatic life ie the best water quality value possible (DEQ 2006b pp 8 33) The table also explains whether the score increases or decreases with environmental stress
As Table 13 indicates each of the eight VSCI metrics is calculated individually Each formula provides a unitless score based on a 100-point scale with 0 as the worst possible score and 100 as the best Any metric that provides a result above 100 is changed to 100 in order for the index to work properly After the eight metrics are calculated they are summed and averaged to provide a final VSCI score on a 100-point scale
Based on statistical analysis of Virginiarsquos streams and their BMI communities the DEQ (2006b p 8) currently uses the following tiers to interpret VSCI scores
VSCI Scores Aquatic Life Use
(ALI) Tiers
lt 42 severely stressed
42-55 moderately stressed
55-63 unknownfair (gray zone)
63-73 good
gt73 excellent
Section 2 of this report presents the results of calculations for the six BMI sampling sites the research team studied in the Hiatt-Lick Run subwatershed in 2008
o
p
1 Total taxa the number of distinct taxa in the sample measures the overall diversity of the macroinvertebrate assemblage The best standard value (BSV) is 189 Score = 100 x (X189) Total taxa decreases with stress 2 EPT taxa the sum of the distinct taxa in the orders Ephemeroptera (mayflies) Plecoptera (stoneflies) and Trichoptera (caddisflies) These orders are known to be sensitive to pollution The best standard value (BSV) is 117 Score = 100 x (X117) EPT taxa decreases with stress 3 Percent Ephemeroptera the percent abundance of individuals in the sample that belong to the order Ephemeroptera (mayflies) The best standard value (BSV) is 477 Score = 100 x (X477) Percent Ephemeroptera decreases with stress 4 Percent Plecoptera + Trichoptera less Hydropsychidae the percent abundance of individuals in the sample that belong to the orders Plecoptera (stoneflies) and Trichoptera (caddisflies) excluding the pollution-tolerant caddisfly family Hydropsychidae The best standard value (BSV) is 562 Score = 100 x (X562) Percent Plecoptera + Trichoptera less Hydropsychidae decreases with stress 5 Percent Scrapers the percent abundance of individuals in the sample whose primary functional mechanism for feeding is grazing on the substrate or periphyton (ie attached algae and associated material) The best standard value (BSV) is 467 Score = 100 x (X467) Percent scrapers decreases with stress 6 Percent Chironomidae the percent abundance of individuals in the sample that are Chironomidae (non-biting midge) larvae and pupae The best standard value (BSV) is 16 Score = 100 x [(100-X)(100-16)] Percent Chironomidae increases with stress 7 Percent Top Two Dominant the percent abundance of the two most frequent taxa present in the sample The best standard value (BSV) is 328 Score = 100 x [(100-X)(100-328)] The Percent Top 2 Dominant increases with stress 8 Hilsenhoff Biotic Index (HBI) an abundance-weighted average pollution tolerance of all the individuals within a sample keyed to the family level Tolerance ratings range from 1 (highly intolerant) to 10 (highly tolerant) The best standard value (BSV) is 29 Score = 100 x [(10-X)(10-29)] The Hilsenhoff Biotic Index (HBI) increases with stress ______________________________________________________________________________ Table 13 Description of Benthic Macroinvertebrate VSCI Metrics Includes responses (increases decreases) of each VSCI metric to degraded water quality conditions Adapted from Burton and Gerritsen (2003 pp 3-15 3-16 6-2) and DEQ (2008)
q
Appendix C
LABORATORY BENCH SHEETS
Shenandoah Universityrsquos Environmental Studies Department developed the Benthic Macroinvertebrate Laboratory Bench Sheet and VSCI Worksheet to record BMI specimen identifications and their VSCI classifications for each sample
This form is based in part on bench sheets developed by the EPA and Virginia DEQ for recording specimen identifications Additional columns on Shenandoah Universityrsquos forms for relative numbers and VSCI classifications make the calculation and verification of VSCI scores easier
Note the column for taxonomistrsquos initials Specimens were identified by students in Shenandoah Universityrsquos research group and then verified or where necessary changed by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality This external review helps assure the validity of the study Originals of these bench sheets with handwritten taxonomistsrsquo initials will be kept on file at Shenandoah Universityrsquos Environmental Studies Department for five years after the completion of this study
r
s
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-1 STATION NAME Mouth LAT N 39deg 12749rsquo (upstream) LONG W 078deg 04209rsquo (upstream) LAT N 39deg 12729rsquo (downstream) LONG W 078deg 04148rsquo (downstream) FORM COMPILED BY Jeremy Tovar W S Bousquet RIVER BASIN Potomac River INVESTIGATORS Marie Dahl Brandon Millholand J W Pangle Sean Robertson Jeremy Tovar Woodward Bousquet Ben Bousquet
Collection date 21 May 2008 Sheet completion date 12 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Hirudinea 3 33 JT BVW PR 8 SP Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Coenagrionidae 2 22 BVW PR 9 CR Ephemeroptera (mayflies) Caenidae 1 11 JT BVW CG 6 SP Ephemerllidae 4 44 JT BVW CG 2 CR Heptageniidae 5 55 JT BVW SC 3 CG Isonychiidae 1 11 BVW CF 3 CG Plecoptera (stoneflies) Perlidae 14 154 JT BVW PR 2 CR Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Corydalidae 1 11 JT BVW PR 5 CR Coleoptera (beetles) Elmidae 24 264 I JT BVW SC 4 CG Dytiscidae 4 44 JT BVW PR 6 GN Psephenidae 3 33 JT BVW SC 4 CG Diptera (true flies) Tipulidae 3 33 JT BVW SH 4 BU Chironomidae 26 286 JT BVW CG 6 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
t
Other Total Individuals 91 1001 Total Taxa 13
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
u
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-1 STATION NAME Mouth LAT N 39deg 12749rsquo (upstream) LONG W 078deg 04209rsquo (upstream) LAT N 39deg 12729rsquo (downstream) LONG W 078deg 04148rsquo (downstream) FORM COMPILED BY Jeremy Tovar W S Bousquet RIVER BASIN Potomac River INVESTIGATORS Brandon Millholand JW Pangle Jeremy Tovar Heather Kepple
Collection date 10 October 2008 Sheet completion date 12 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Oligochaeta 3 35 JT BVW CG 8 BU Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Coenagrionidae 2 23 JT BVW PR 9 CR Ephemeroptera (mayflies) Heptageniidae 12 140 JT BVW SC 3 CG Isonychiidae 2 23 BVW CF 3 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Hydropsychidae 16 186 JT BVW CF 6 CG Philopotamidae 31 360 JT BVW CF 2 CG Hemiptera (true bugs) (Veliidae) (5) - JT BVW ( ) Megaloptera (dobsonflies etc) Corydalidae 10 116 JT BVW PR 5 CR Coleoptera (beetles) Dytiscidae 1 12 JT BVW PR 6 GN Psephenidae 7 81 JT BVW SC 4 CG Diptera (true flies) Tipulidae 2 23 BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
v
Other Total Individuals 86
(91) 999
Total Taxa 10 (11)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009 Veliidae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
w
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-2 STATION NAME Package Plant LAT N 39deg 12958rsquo (upstream) LONG W 078deg 04900 (upstream) LAT N 39deg 12958rsquo (downstream) LONG W 078deg 04822 (downstream) FORM COMPILED BY JW Pangle W Bousquet RIVER BASIN Potomac River INVESTIGATORS JW Pangle S Robertson J Tovar M Dahl B Millholland W Bousquet
Collection date 29 May 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Plecoptera (stoneflies) Perlidae 11 136 I JP BVW PR 1 CR Trichoptera (caddisflies) Hydropsychidae 10 123 I JP BVW CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 14 173 I JP BVW SC 4 CG Diptera (true flies) Chironomidae 32 395 I JP BVW CG 6 BU Simuliidae 14 173 I JP BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
x
Other Total Individuals 81 1000 Total Taxa 5
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-2 STATION NAME Package Plant LAT N 39deg 12958rsquo (upstream) LONG W 078deg 04900 (upstream) LAT N 39deg 12958rsquo (downstream) LONG W 078deg 04822 (downstream) FORM COMPILED BY JW Pangle W Bousquet RIVER BASIN Potomac River INVESTIGATORS JW Pangle Brandon Millholland Jeremy Tovar Heather Kepple
Collection date 10 October 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Heptageniidae 40 392 I WBJP SC 3 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Hydropsychidae 7 69 I JP WB BVW CF 6 CG Philopotamidae 28 275 I JP WB BVW CF 2 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Corydalidae 6 59 I JP WB BVW PR 5 CR Coleoptera (beetles) Psephenidae 7 69 I JP WB BVW SC 4 CG Elmidae 8 78 I JP WB BVW SC 4 CG Diptera (true flies) Tipulidae 6 59 I JP WB BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
y
z
Other Total Individuals 102 1001 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-3 STATION NAME Rt 664 Bridge LAT N 39deg 12934rsquo (upstream) LONG W 078deg 05019rsquo (upstream) LAT N 39deg 12951rsquo (downstream) LONG W 078deg 04958rsquo (downstream) FORM COMPILED BY S Robertson W Bousquet RIVER BASIN Potomac River INVESTIGATORS SRobertson BMillholland WBousquet MDahl JPangle
Collection date 28 May 2008 Sheet completion date 11 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Leptophlebiidae 1 15 I SGR BVW CG 2 CR Baetidae 1 15 I BVW CG 5 CG Plecoptera (stoneflies) Perlidae 3 46 I SGR BVW PR 1 CR Nemouridae 1 15 I BVW SH 3 CR Trichoptera (caddisflies) Hydropsychidae 5 77 I SGR BVW CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 27 415 I SGR BVW SC 4 CG Psephenidae 2 31 A SGR BVW SC 4 CG Diptera (true flies) Simuliidae 5 77 I SGR BVW CF 6 CG Chironomidae 20 308 I SGR BVW CG 6 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
aa
bb
Other Total Individuals 65 999 Total Taxa 9
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-3 STATION NAME Rt 664 Bridge LAT N 39deg 12934rsquo (upstream) LONG W 078deg 05019rsquo (upstream) LAT N 39deg 12951rsquo (downstream) LONG W 078deg 04958rsquo (downstream) FORM COMPILED BY S Robertson RIVER BASIN Potomac River INVESTIGATORS S Robertson M Dahl A Fisher H Kepple
Collection date 10 Oct 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Heptageniidae 2 20 I SGR SC 3 CG Isonychiidae 2 20 I BVW CF 2 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Philopotamidae 36 367 I SGR CF 2 CG Hydropsychidae 47 480 I SGR CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 2 20 A SGR SC 4 CG Psephenidae 8 82 A SGR SC 4 CG Diptera (true flies) Tabanidae 1 10 I SGR PR 7 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
cc
dd
Other Total Individuals 98 999 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-4 STATION NAME Unnamed Tributary LAT (upstream) N 39deg 13035rsquo LONG (upstream) W 078deg 05129rsquo LAT (downstream) N 39deg 13000rsquo LONG (downstream) W 078deg 5086rsquo FORM COMPILED BY A Fisher M Dahl rev W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson A Fisher H Kepple
Collection date 31 May 2008 Sheet completion date rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Flatworms (Turbellaria) Worms (Oligochaeta) Naididae 1 14 - BVW CG 8 BU Leeches (Hirudinea) Snails (Gastropoda) Clams (Bivalvia) Isopods (Isopoda) Asellidae 3 42 A AF BVW CG 8 SP Scuds (Amphipoda) Crayfish Shrimp (Decapoda) Water Mites (Hydracarina) Dragonflies (Odonata Anisoptera) Damselflies (Odonata Zygoptera) Mayflies (Ephemeroptera) Stoneflies (Plecoptera) Perlidae 2 28 I AF BVW PR 1 CR Caddisflies (Trichoptera) True Bugs (Hemiptera) Corixidae 1 14 I AF BVW MP 9 DV Dobsonflies (Megaloptera) Beetles (Coleoptera) Dytiscidae 1 14 I AF BVW PR 6 GN True Flies (Diptera) Chironomidae 61 847 I AF BVW CG 6 BU Simuliidae 3 42 I AF BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
ee
ff
Other Total Individuals 72 1001 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-4 STATION NAME Unnamed Tributary LAT (upstream) N 39deg 13035rsquo LONG (upstream) W 078deg 05129rsquo LAT (downstream) N 39deg 13000rsquo LONG (downstream) W 078deg 5086rsquo FORM COMPILED BY Andrew G M Fisher W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson A Fisher H Kepple
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Flatworms (Turbellaria) Worms (Oligochaeta) Lumbriculidae 2 32 A AF BVW CG 8 BU Leeches (Hirudinea) Snails (Gastropoda) Lymnaeidae 1 16 A BVW CG 9 GN Clams (Bivalvia) Isopods (Isopoda) Scuds (Amphipoda) Crayfish Shrimp (Decapoda) Cambaridae 1 16 A AF BVW GN 5 GN Water Mites (Hydracarina) Dragonflies (Odonata Anisoptera) Damselflies (Odonata Zygoptera) Calopterygidae 4 63 I BVW PR 7 CL Mayflies (Ephemeroptera) Tricorythidae 1 16 I BVW CG 5 SP Stoneflies (Plecoptera) Caddisflies (Trichoptera) Phryganeidae 4 63 I AF BVW SH 4 CR Hydropsychidae 6 95 I AF BVW CF 6 CG I True Bugs (Hemiptera) Corixidae 8 127 I AF BVW MP 9 DV (Veliidae ) (7) - I AF BVW ( ) Dobsonflies (Megaloptera) Sialidae 3 48 I AF BVW PR 7 SP Corydalidae 2 32 I AF BVW PR 5 CR Beetles (Coleoptera) Haliplidae 1 16 A AF BVW MP 8 GN Dysticidae 7 111 I AF BVW PR 6 GN Hydrophilidae 1 16 A AF BVW PR 8 GN Elmidae 3 48 I BVW SC 4 CG True Flies (Diptera) Chironomidae 8 127 I AF BVW CG 6 BU Simuliidae 7 111 I AF BVW CF 6 CG Tipulidae 4 63 I AF BVW SH 4 GU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
gg
hh
Other Total Individuals 63
(70) 1000
Total Taxa 17 (18)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Veliidae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-5 STATION NAME Lick Run LAT N 39deg 12752rsquo (upstream) LONG W 078deg 05685rsquo (upstream) LAT N 39deg 12784rsquo (downstream) LONG W 078deg 05631rsquo (downstream) FORM COMPILED BY Brandon Millholland W Bousquet RIVER BASIN Potomac River INVESTIGATORS J Pangle S Robertson W Bousquet JTovar M Dahl
Collection date 29 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Gomphidae 1 15 I BVW PR 3 BU Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Leptophlebiidae 11 167 A LBM BVW CG 2 CR Plecoptera (stoneflies) Perlidae 10 152 A LBM BVW PR 1 CR Perlodiae 1 15 A LBM BVW PR 2 CR Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 5 76 A LBM BVW SC 4 CG Elmidae 2 30 I LBM BVW SC 4 CG Diptera (true flies) (Culicidae) (3) - P LMB BVW ( ) Chironomidae 23 348 I LBM BVW CG 6 BU Simuliidae 13 197 I LBM BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
ii
jj
Other Total Individuals 66
(69) 1000
Total Taxa 7 (8)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Culicidae (Diptera) pupae not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl J Pangle S Robertson W Bousquet B Millholland J Tovar H Kepple
Collection date 30 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 1 12 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Asellidae 114 965 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Plecoptera (stoneflies) Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 2 23 A MRD BVW SC 4 CG Diptera (true flies)
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
kk
ll
Other Total Individuals 117 1000 Total Taxa 3
Notes Subsample taken 29 October 2009 All taxonomic determinations by MRD were confirmed by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson K McCann A Fisher H Kepple A Barrett
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 2 17 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Sphaeriidae 2 17 I MRD BVW CF 8 BU Isopoda (isopods) Asellidae 99 853 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Calopterygidae 2 17 A MRD BVW PR 7 CL Ephemeroptera (mayflies) Leptophlebiidae 2 17 I MRD BVW CG 2 CR Heptageniidae 4 34 A MRD BVW SC 3 CG Plecoptera (stoneflies) Perlidae 1 09 A MRD BVW PR 1 CR Trichoptera (caddisflies) Hemiptera (true bugs) (Gerridae) (1) - A MRD BVW ( ) Corixidae 1 09 A MRD BVW PR 5 CR Megaloptera (dobsonflies etc) Coleoptera (beetles) Dryopidae 1 09 A MRD BVW SC 5 CG Diptera (true flies) Tipulidae 2 17 I BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
mm
nn
Other Total Individuals 116
(117) 999
Total Taxa 10 (11)
Notes Subsample taken 6 November 2008 Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009 Gerridae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
- Disclaimer ii
- Title Page iii
- Acknowledgements v
- Table of Contents vii
- SECTION 2 RESULTS DATA TABLES 7
- OVERVIEW AND FINDINGS
- Introduction
- RESULTS DATA TABLES
- REFERENCES
- FIELD DATA SHEETS
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- SubstrateType
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- WATER QUALITY ANALYSIS
- THROUGH THE VIRGINIA
- STREAM CONDITION INDEX (VSCI)
- LABORATORY BENCH SHEETS
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
Section 2
RESULTS DATA TABLES
Site HLR-1 Mouth
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 13 189 6872 EPT taxa 5 117 4273 Ephemeroptera 121 477 2544 Plec +Tric less Hydropsych 154 562 2745 Scrapers 352 467 7536 Chironomidae 286 16 7267 Top 2 Dominant 550 328 6708 Hilsenhoff Biotic Index (HBI) 45 29 775VSCI Score 571
According to the DEQrsquos water quality standards aquatic life at this site is fair (gray zone) See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 2 VSCI Scores for Mouth (HLR-1) Site in Hiatt-Lick Run Sampled on 21 May 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 10 189 5292 EPT taxa 4 117 3423 Ephemeroptera 163 477 3424 Plec +Tric less Hydropsych 360 562 6415 Scrapers 221 467 4736 Chironomidae 00 16 10007 Top 2 Dominant 546 328 6768 Hilsenhoff Biotic Index (HBI) 39 29 859VSCI Score 608
According to the DEQrsquos water quality standards aquatic life at this site is fair (gray zone) See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 3 VSCI Scores for Mouth (HLR-1) Site in Hiatt-Lick Run Sampled 10 October 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
7
Site HLR-2 Package Plant
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 5 189 2642 EPT taxa 2 117 1713 Ephemeroptera 00 477 004 Plec +Tric less Hydropsych 110 562 1965 Scrapers 173 467 3706 Chironomidae 395 16 6157 Top 2 Dominant 568 328 6438 Hilsenhoff Biotic Index (HBI) 50 29 704VSCI Score 370
According to the DEQrsquos water quality standards aquatic life at this site is severely stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 4 VSCI Scores for Package Plant (HLR-2) Site in Hiatt-Lick Run Sampled 29 May 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 7 189 3702 EPT taxa 34 117 2563 Ephemeroptera 392 477 8214 Plec +Tric less Hydropsych 275 562 4895 Scrapers 539 467 10006 Chironomidae 00 16 10007 Top 2 Dominant 667 328 4968 Hilsenhoff Biotic Index (HBI) 32 29 958VSCI Score 674
According to the DEQrsquos water quality standards aquatic life at this site is good See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 5 VSCI Scores for Package Plant (HLR-2) Site in Hiatt-Lick Run Sampled 10 October 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
8
Site HLR-3 Route 664 Bridge
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 9 189 4762 EPT taxa 5 117 4273 Ephemeroptera 30 477 634 Plec +Tric less Hydropsych 61 562 1095 Scrapers 446 467 9556 Chironomidae 308 16 7037 Top 2 Dominant 723 328 4128 Hilsenhoff Biotic Index (HBI) 48 29 732VSCI Score 485
According to the DEQrsquos water quality standards aquatic life at this site is moderately stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 6 VSCI Scores for Route 664 Bridge (HLR-3) Site in Hiatt-Lick Run Sampled 28 May 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 7 189 3702 EPT taxa 4 117 3423 Ephemeroptera 40 477 844 Plec +Tric less Hydropsych 367 562 6535 Scrapers 122 467 2616 Chironomidae 00 16 10007 Top 2 Dominant 847 328 2288 Hilsenhoff Biotic Index (HBI) 42 29 817VSCI Score 469
According to the DEQrsquos water quality standards aquatic life at this site is moderately stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 7 VSCI Scores for Route 664 Bridge (HLR-3) Site in Hiatt-Lick Run Sampled 10 October 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
9
Site HLR-4 Unnamed Tributary
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 7 189 3702 EPT taxa 1 117 853 Ephemeroptera 00 477 004 Plec +Tric less Hydropsych 28 562 495 Scrapers 00 467 006 Chironomidae 847 16 1557 Top 2 Dominant 889 328 1658 Hilsenhoff Biotic Index (HBI) 60 29 563VSCI Score 173
According to the DEQrsquos water quality standards aquatic life at this site is severely stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 8 VSCI Scores for Unnamed Tributary (HLR-4) Site in Hiatt-Lick Run Sampled 31 May 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 17 189 8992 EPT taxa 3 117 2563 Ephemeroptera 16 477 344 Plec +Tric less Hydropsych 63 562 1125 Scrapers 48 467 1036 Chironomidae 127 16 8877 Top 2 Dominant 254 328 10008 Hilsenhoff Biotic Index (HBI) 63 29 521VSCI Score 476
According to the DEQrsquos water quality standards aquatic life at this site is moderately stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 9 VSCI Scores for Unnamed Tributary (HLR-4) Site in Hiatt-Lick Run Sampled 10 October 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
10
Site HLR-5 Lick Run
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 7 189 3702 EPT taxa 3 117 2563 Ephemeroptera 167 477 3504 Plec +Tric less Hydropsych 167 562 2975 Scrapers 106 467 2276 Chironomidae 348 16 6637 Top 2 Dominant 545 328 6778 Hilsenhoff Biotic Index (HBI) 43 29 803VSCI Score 455
According to the DEQrsquos water quality standards aquatic life at this site is moderately stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 10 VSCI Scores for Lick Run (HLR-5) Site in Hiatt-Lick Run Sampled 29 May 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
Note
Water conditions at the Lick Run (HLR-5) site were too low in October 2008 to permit a sample to be taken
11
Site HLR-6 McCann Farm
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 3 189 1592 EPT taxa 0 117 003 Ephemeroptera 00 477 004 Plec +Tric less Hydropsych 00 562 005 Scrapers 23 467 496 Chironomidae 00 16 10007 Top 2 Dominant 988 328 188 Hilsenhoff Biotic Index (HBI) 79 29 296VSCI Score 190
According to the DEQrsquos water quality standards aquatic life at this site is severely stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 11 VSCI Scores for McCann Farm (HLR-6) Site in Hiatt-Lick Run Sampled 30 May 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 10 189 5292 EPT taxa 3 117 2563 Ephemeroptera 51 477 1074 Plec +Tric less Hydropsych 09 562 165 Scrapers 43 467 926 Chironomidae 00 16 10007 Top 2 Dominant 887 328 1688 Hilsenhoff Biotic Index (HBI) 75 29 352VSCI Score 315
According to the DEQrsquos water quality standards aquatic life at this site is severely stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 12 VSCI Scores for McCann Farm (HLR-6) Site in Hiatt-Lick Run Sampled 10 October 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
12
REFERENCES
Barbour Michael T Jeroen Gerritsen Blaine D Snyder and James B Stribling 1999 Rapid bioassessment protocols for use in streams and wadeable rivers periphyton benthic macroinvertebrates and fish Second edition EPA 841-B-99-002 Washington DC US Environmental Protection Agency Office of Water Available online at httpwwwepagovOWOWmonitoringbioasspshtml Burton June and Jeroen Gerritsen 2003 A stream condition index for Virginia non-coastal streams Prepared for the US EPA Office of Science and Technology US EPA Region 3 Environmental Services Division and Virginia Department of Environmental Quality Owings Mills Maryland Tetra Tech Inc Available online at httpwwwdeqvirginiagovwatermonitoringpdf vastrmconpdf Hessler Rachel A Andrea R Martin Heidi D Ruggeri William B Seymour Jr and Woodward S Bousquet 2006 Water quality assessment using benthic macroinvertebrates A study and compilation Winchester Virginia Environmental Studies Program Shenandoah University Occasional publication no 10 Igoe Robert 2006 DEQ identifies nine impaired streams in Clarke Frederick The Winchester Star 15 July B1 Jackson Charlie 2005 Survey targets Opequon cleanup The Winchester Star 19 August B1 Johnston Elizabeth A and Woodward S Bousquet 2003 What happens when it rains A study of water quality in Abrams Creek and Town Run Winchester and Frederick County Virginia Winchester Virginia Environmental Studies Program Shenandoah University Occasional publication no7 Merritt Richard W and Kenneth W Cummins 1996 An introduction to the aquatic insects of North America Dubuque Iowa KendallHunt Publishing Company Opequon Creek IP Steering Committee Virginia Tech Department of Biological Systems Engineering and Center for TMDL and Watershed Studies University of Virginia Institute for Environmental Negotiation and West Virginia University Division of Resource Management 2006 Opequon Creek watershed TMDL implementation plan Blacksburg Virginia Virginia Tech Department of Biological Systems Engineering Available online at httpwwwtmdlbsevteduoutreachC82 Pennak Robert W 1953 Fresh-water invertebrates of the United States New York New York The Ronald Press Company Rosenburg David M and Vincent H Resh 1993 Freshwater biomonitoring and benthic macroinvertebrates New York New York Chapman amp Hall Shenandoah University Public Relations Office 2002 Data collected by SU and its partners helps identify impaired water Winchester Virginia Available online at httpwwwsuedutemp_ newscfmurlnum=32 Thorp James H and Alan P Covich 1991 Ecology and classification of North American freshwater invertebrates San Diego California Academic Press Inc
13
US Environmental Protection Agency 1997 Volunteer stream monitoring a methods manual EPA 841-B-97-003 Washington DC US Environmental Protection Agency Office of Water Van Meter Val 2003 Opequon Creek pollution limits to be established The Winchester Star 4 April B1 Virginia Department of Environmental Quality 2006a Virginia 305(b)303(d) water quality integrated report to Congress and the EPA Administrator for the period January 1 2000 to December 31 2004 Richmond Virginia Virginia Department of Environmental Quality Available online at httpwwwdeqvirginiagovwqair2006html __________ 2006b Using probabilistic data to validate the non-coastal Virginia Stream Condition Index (draft) VDEQ technical bulletin WQA2006-001 Virginia Department of Environmental Quality Available online at httpwwwloudounwatershedorgstream_assessmentreference_documents VA_DEQ_SCI_Validationpdf __________ 2008 Biological monitoring program quality assurance project plan for wadeable streams and rivers Richmond Virginia Virginia Department of Environmental Quality Update available online at httpwwwdeqstatevausbiomonitoringdocumentsBioMonQAPP_13Aug2008pdf Virginia Tech Aquatic Entomology Laboratory 2005 Taxa dictionary [Excel file] Blacksburg Virginia Virginia Tech Department of Biological Systems Engineering 2003a Opequon watershed TMDLs for benthic impairments Abrams Creek and Lower Opequon Creek Frederick and Clarke Counties Virginia Prepared for the Virginia Department of Environmental Quality and Virginia Department of Conservation and Recreation Blacksburg Virginia Available online at httpwwwdeqstate vaustmdlapptmdlsshenrvrabropebcpdf Voshell J Reese Jr 2002 A guide to common freshwater invertebrates of North America Blacksburg Virginia The McDonald amp Woodward Publishing Company
14
APPENDICES
15
16
a
Appendix A
FIELD DATA SHEETS
This appendix presents a Site Characterization Field Data Sheet for each of the six sampling reaches established on Hiatt Run and Lick Run for this study They were filled out during the sampling runs made in May 2008
These forms were adapted by Shenandoah University from forms used by the EPA and Virginia DEQ for recording characteristics of sampling sites
b
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Mouth STATION _HLR-1_____ STREAM CLASS LAT N 39 12749 (upstream) LONG W 078 04209(upstream) LAT N 39 12729 (downstream) LONG W 078 04148 (downstream) FORM COMPILED BY Jeremy Tovar RIVER BASIN Potomac River INVESTIGATORS JPangle B Millholland MDahl SRobertson W Bousquet JTovar BBousquet
DATE May 21 2008_________ TIME_250 PM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature __24___degC showers (intermittent) Comments _______________________
___ cloud cover _0_ ______________________________
X clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x ( Significant springs andor seeps) x (Valley ) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS
c
d
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence x FieldPasture Industrial Obvious sources Agricultural Other ____________ Stream scour steep slope erosion x Residential Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left Field and young thin deciduous forest 100 Bank=Grass cobble and shrub Right 90 maturing deciduous forest 45 shale slope Bank=Bedrock soil and herbs Indicate the dominant vegetation type(s) and record the dominant species
x Trees Shrubs x (Grasses) Forbs Dominant species Oak species and mix deciduous
INSTREAM FEATURES
Measured reach length ___100__m Canopy Cover Estimated stream width _35__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area __350_m2
Estimated stream depth _008_m Proportion of Reach Represented by Stream High water mark _15_m Morphology Types Surface velocity (at thalweg) _05__msec x Riffle__75___ x Run__20___ x Pool__5___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___00__ m2 Density of LWD _-_ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __15_____ x Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present Algae
WATER QUALITY Temperature __19___degC Water Odors x NormalNone Sewage Specific Conductance - Petroleum Chemical Fishy Other___________________ Dissolved Oxygen - Water Surface Oils Slick Sheen Globs Flecks pH __-_ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used _____NA________ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic x None Relict shells Other______None_____ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 8 Detritus sticks wood coarse plant materials
0 Boulder gt256 mm (10rsquorsquo) 0
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 51 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 40 Sand 006-2 mm (gritty) 1 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University
SITE CHARACTERIZATION FIELD DATA SHEET FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Package Plant STATION HLR-2 STREAM CLASS LAT N 39 129580 (upstream) LONG W 078 04900 (upstream) LAT N 39 129581 (downstream) LONG W 078 04822 (downstream) FORM COMPILED BY JW Pangle RIVER BASIN Potomac River INVESTIGATORS JPangle JTovar MDahl B Millholland SRobertson W Bousquet
DATE__19 May 2008_ TIME__1240__ PM
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature _____degC showers (intermittent) Comments __Sunny Day_____________
_5__ cloud cover _0__ ______________________________
x clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x (Significant springs andor seeps) x (Valley) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS
e
f
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence FieldPasture Industrial Obvious sources Agricultural Other ____________ __Upstream Construction and Stream bank Scour Residential __________________________________ Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left Deciduous forest with thick herb and shrub layers bank= vegetation and rocks Right Deciduous forest with thick herb and shrub layers bank=vegetation soil and rocks Indicate the dominant vegetation type(s) and record the dominant species
x Trees x Shrubs Grasses Forbs Dominant species Black Walnut White Ash Hackberry and American Sycamore
INSTREAM FEATURES
Measured reach length 100 m Canopy Cover Estimated stream width _35__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area __350_m2
Estimated stream depth __15__m Proportion of Reach Represented by Stream High water mark __22___m Morphology Types Surface velocity (at thalweg) _035__msec x Riffle__30___ x Run__30___ x Pool__40___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___10____ m2 Density of LWD _______ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __30___ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present__________________________________________________________
WATER QUALITY Temperature __18___degC Water Odors x NormalNone Sewage Specific Conductance __-____ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ____-____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used _____NA____ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other___None_____ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach SubstrateType Characteristic
Composition in Sampling Reach
Bedrock 3 Detritus sticks wood coarse plant materials
2 Boulder gt256 mm (10rsquorsquo) 5
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 70 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 22 Sand 006-2 mm (gritty) 0 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiattrsquos Run LOCATION Rt 664 Bridge STATION HLR-3 STREAM CLASS LAT N 39 12934 (upstream) LONG W 078 05019 (upstream) LAT N 39 12958 (downstream) LONG W 078 04958 (downstream) FORM COMPILED BY Sean Robertson RIVER BASIN Potomac River INVESTIGATORS SRobertson BMillholland WBousquet MDahl JPangle
DATE 28 May 2008 TIME 1127 AM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality 2008
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature __14___degC showers (intermittent) x Trace Comments _______________________
_65 x cloud cover ___ ______________________________
x clearsunny __________________________________
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x (Significant springs andor seeps) x (Valley) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS
g
h
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence Some evidence x FieldPasture Industrial x Obvious sources Agricultural x Other Old Package Plant Downstream x Residential Bridge and Road Power line and Pump station Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left 70 field 30 shrubs and deciduous trees Bank=Soil and vegetation Right Maturing deciduous forest steep slope Bank=Shale Bedrock Indicate the dominant vegetation type(s) and record the dominant species
x Trees x Shrubs x Grasses Forbs Dominant species Sycamore and Red Oaks
INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width __51___m Fully open x Partly open Partly shaded Fully shaded Sampling reach area _510_m2
Estimated stream depth __135___m Proportion of Reach Represented by Stream High water mark __-___m Morphology Types Surface velocity (at thalweg) __20___msec x Riffle_40_ x Run_50_ x Pool 10 Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___0____ m2 Density of LWD ___-____ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation ___50____ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present Brown Algae
WATER QUALITY Temperature __17___degC Water Odors x NormalNone Sewage Specific Conductance ___-___ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ___-_____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used ____NA_______ Clear x Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other____None____ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 15 Detritus sticks wood coarse plant materials
0 Boulder gt256 mm (10rsquorsquo) 15
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 62 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 8 Sand 006-2 mm (gritty) 0 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Unnamed Tributary STATION HLR-4 STREAM CLASS LAT N 39 13035 (upstream) LONG W 078 05129 (upstream) LAT N 39 13000 (downstream) LONG W 078 05086 (downstream) FORM COMPILED BY Marie Dahl RIVER BASIN Potomac River INVESTIGATORS M Dahl HKepple SRobertson AFisher
DATE 31 May 2008 TIME 5 PM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality 2008
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) YES x NO
rain (steady rain) Air Temperature __22___degC showers (intermittent) Comments _______________________
_0__ cloud cover _0__ ______________________________
x clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area __-___ km2 mi2 Montane Significant springs andor seeps x Valley Marsh or swamp Montane amp Valley Mixture of origins Other ___________________
COMMENTS Snowden Bridge development (upstream) and sewer line
i
j
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence x FieldPasture Industrial Obvious sources Agricultural Other ____________ Construction upstream and adjacent x Residential Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left Forested with rocky banks field across silt fence and 2m tree strip Right Forested with some rocky banks and forest through the highway Indicate the dominant vegetation type(s) and record the dominant species
x Trees x Shrubs x Grasses Forbs Dominant species White Ash and Hackberry
INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width ___38__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area __380__m2
Estimated stream depth __12__cm Proportion of Reach Represented by Stream High water mark __2___m Morphology Types Surface velocity (at thalweg) __46___msec x Riffle__25___ x Run__70___ x Pool__5___ Channelized Yes x No Dam Present x Yes No
LARGE WOODY DEBRIS
LWD _______ m2 Density of LWD _______ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation ___NA____ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present___Algae___
WATER QUALITY Temperature __16___degC Water Odors x NormalNone Sewage Specific Conductance __-____ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ___-_____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used ____NA_____ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells x Other___Shale_______ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 0 Detritus sticks wood coarse plant materials
10 Boulder gt256 mm (10rsquorsquo) 5
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 45 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 30 Sand 006-2 mm (gritty) 0 Marl grey shell
fragments 0
Silt 0004-006 mm 20 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Lick Run STATION HLR-5 STREAM CLASS LAT N 39 127517 (upstream) LONG W 078 05685(upstream) LAT N 39 12784 (downstream) LONG W 078 05631 (downstream) FORM COMPILED BY B Millholland RIVER BASIN Potomac River INVESTIGATORS B Millholland JTovar SRobertson MDahl WBousquet JPangle
DATE 29 May 2008 TIME 1040 AM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project
k
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature __15___degC showers (intermittent) Comments _______________________
_5_ X cloud cover _0__ ______________________________
x clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane Significant springs andor seeps x Valley Marsh or swamp Montane amp Valley Mixture of origins Other ___________________
COMMENTS Lick Run appears to be intermittent and may be dry in Fall 2008
l
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence x FieldPasture Industrial Obvious sources Agricultural Other ____________ Adjacent logging stream bank scour Residential Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left 85 young deciduous forest 15 logged Bank=Soil vegetation and cobbles Right Young deciduous forest on 30 slope Bank= Soil vegetation and cobble Indicate the dominant vegetation type(s) and record the dominant species
x Trees Shrubs Grasses Forbs Dominant species Mixed Deciduous INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width __11__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area 110 m2
Estimated stream depth _009__m Proportion of Reach Represented by Stream High water mark __96___m Morphology Types Surface velocity (at thalweg) __01___msec x Riffle__30___ x Run__5___ x Pool__65___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___0____ m2 Density of LWD ___-____ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __70___ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present__________________________________________________________
WATER QUALITY Temperature __14___degC Water Odors x NormalNone Sewage Specific Conductance __-__ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ___-___ Water Surface Oils Slick Sheen Globs Flecks pH __-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used _____NA______ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other_____None_____
SEDIMENT AND SUBSTRATE
Odors Deposits Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other________________ x Other Slightly Salty Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 0 Detritus sticks wood coarse plant materials
5 Boulder gt256 mm (10rsquorsquo) 0
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 75 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 23 Sand 006-2 mm (gritty) 2 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION McCann Farm STATION HLR-6 STREAM CLASS LAT N 39 13076 (upstream) LONG W 078 06539 (upstream) LAT N 39 13063 (downstream) LONG W 078 06473 (downstream) FORM COMPILED BY MDahl RIVER BASIN Potomac River INVESTIGATORS MDahl JPangle SRobertson W Bousqet BMillholland JTovar H Kepple
DATE 30 May 2008 TIME 1050 AM (start)
PROJECT NAME
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) YES x NO
rain (steady rain) Air Temperature __18___degC showers (intermittent) Comments _______________________
_85_x cloud cover _10__ ______________________________
clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x (Significant springs andor seeps) x ( Valley) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS Multi-habitat sampling with D-frame nets and seine
m
n
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial x No evidence Some evidence x FieldPasture Industrial Obvious sources x Agricultural Other ____________ Minor stream bank scour Residential Local Watershed Erosion x None Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left 35 bank grasses scattered trees and some bushes Right High bank with grasses and scattered trees Indicate the dominant vegetation type(s) and record the dominant species
x Trees Shrubs Grasses Forbs Dominant species Black Walnut and Festuca elatior
INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width __2___m Fully open Partly open x Partly shaded Fully shaded Sampling reach area __200_m2
Estimated stream depth __03_m Proportion of Reach Represented by Stream High water mark __1___m Morphology Types Surface velocity (at thalweg) __166_msec x Riffle__0___ x Run__55___ x Pool__45___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___2____ m2 Density of LWD ___-____ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __20_____ x Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present ____Glyceria (grass)sp__________
WATER QUALITY Temperature __16___degC Water Odors x NormalNone Sewage Specific Conductance ___-___ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ____-____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used ______NA___ Clear x Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other__NONE__ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 0 Detritus sticks wood coarse plant materials
5 Boulder gt256 mm (10rsquorsquo) 0
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 27 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 38 Sand 006-2 mm (gritty) 5 Marl grey shell
fragments 0 (lt1)
Silt 0004-006 mm 25 Clay lt 0004 mm (slick) 5 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Appendix B
WATER QUALITY ANALYSIS THROUGH THE VIRGINIA
STREAM CONDITION INDEX (VSCI)
The Virginia Stream Condition Index (VSCI) relies on eight metrics to categorize stream health According to Burton and Gerritsen (2003 p 1-1) this index ldquohellip can be used as a primary indicator of ecosystem health and can identify impairment with respect to the reference (or natural) conditionrdquo These metrics are numeric measures of a biological communityrsquos species composition diversity ecological niches or tolerance to pollution
Table 13 on the next page describes each VSCI metric lists its formula and provides the best standard value (BSV) for that metric Based on a 100-point scale the BSV is the score that reflects optimum conditions for aquatic life ie the best water quality value possible (DEQ 2006b pp 8 33) The table also explains whether the score increases or decreases with environmental stress
As Table 13 indicates each of the eight VSCI metrics is calculated individually Each formula provides a unitless score based on a 100-point scale with 0 as the worst possible score and 100 as the best Any metric that provides a result above 100 is changed to 100 in order for the index to work properly After the eight metrics are calculated they are summed and averaged to provide a final VSCI score on a 100-point scale
Based on statistical analysis of Virginiarsquos streams and their BMI communities the DEQ (2006b p 8) currently uses the following tiers to interpret VSCI scores
VSCI Scores Aquatic Life Use
(ALI) Tiers
lt 42 severely stressed
42-55 moderately stressed
55-63 unknownfair (gray zone)
63-73 good
gt73 excellent
Section 2 of this report presents the results of calculations for the six BMI sampling sites the research team studied in the Hiatt-Lick Run subwatershed in 2008
o
p
1 Total taxa the number of distinct taxa in the sample measures the overall diversity of the macroinvertebrate assemblage The best standard value (BSV) is 189 Score = 100 x (X189) Total taxa decreases with stress 2 EPT taxa the sum of the distinct taxa in the orders Ephemeroptera (mayflies) Plecoptera (stoneflies) and Trichoptera (caddisflies) These orders are known to be sensitive to pollution The best standard value (BSV) is 117 Score = 100 x (X117) EPT taxa decreases with stress 3 Percent Ephemeroptera the percent abundance of individuals in the sample that belong to the order Ephemeroptera (mayflies) The best standard value (BSV) is 477 Score = 100 x (X477) Percent Ephemeroptera decreases with stress 4 Percent Plecoptera + Trichoptera less Hydropsychidae the percent abundance of individuals in the sample that belong to the orders Plecoptera (stoneflies) and Trichoptera (caddisflies) excluding the pollution-tolerant caddisfly family Hydropsychidae The best standard value (BSV) is 562 Score = 100 x (X562) Percent Plecoptera + Trichoptera less Hydropsychidae decreases with stress 5 Percent Scrapers the percent abundance of individuals in the sample whose primary functional mechanism for feeding is grazing on the substrate or periphyton (ie attached algae and associated material) The best standard value (BSV) is 467 Score = 100 x (X467) Percent scrapers decreases with stress 6 Percent Chironomidae the percent abundance of individuals in the sample that are Chironomidae (non-biting midge) larvae and pupae The best standard value (BSV) is 16 Score = 100 x [(100-X)(100-16)] Percent Chironomidae increases with stress 7 Percent Top Two Dominant the percent abundance of the two most frequent taxa present in the sample The best standard value (BSV) is 328 Score = 100 x [(100-X)(100-328)] The Percent Top 2 Dominant increases with stress 8 Hilsenhoff Biotic Index (HBI) an abundance-weighted average pollution tolerance of all the individuals within a sample keyed to the family level Tolerance ratings range from 1 (highly intolerant) to 10 (highly tolerant) The best standard value (BSV) is 29 Score = 100 x [(10-X)(10-29)] The Hilsenhoff Biotic Index (HBI) increases with stress ______________________________________________________________________________ Table 13 Description of Benthic Macroinvertebrate VSCI Metrics Includes responses (increases decreases) of each VSCI metric to degraded water quality conditions Adapted from Burton and Gerritsen (2003 pp 3-15 3-16 6-2) and DEQ (2008)
q
Appendix C
LABORATORY BENCH SHEETS
Shenandoah Universityrsquos Environmental Studies Department developed the Benthic Macroinvertebrate Laboratory Bench Sheet and VSCI Worksheet to record BMI specimen identifications and their VSCI classifications for each sample
This form is based in part on bench sheets developed by the EPA and Virginia DEQ for recording specimen identifications Additional columns on Shenandoah Universityrsquos forms for relative numbers and VSCI classifications make the calculation and verification of VSCI scores easier
Note the column for taxonomistrsquos initials Specimens were identified by students in Shenandoah Universityrsquos research group and then verified or where necessary changed by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality This external review helps assure the validity of the study Originals of these bench sheets with handwritten taxonomistsrsquo initials will be kept on file at Shenandoah Universityrsquos Environmental Studies Department for five years after the completion of this study
r
s
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-1 STATION NAME Mouth LAT N 39deg 12749rsquo (upstream) LONG W 078deg 04209rsquo (upstream) LAT N 39deg 12729rsquo (downstream) LONG W 078deg 04148rsquo (downstream) FORM COMPILED BY Jeremy Tovar W S Bousquet RIVER BASIN Potomac River INVESTIGATORS Marie Dahl Brandon Millholand J W Pangle Sean Robertson Jeremy Tovar Woodward Bousquet Ben Bousquet
Collection date 21 May 2008 Sheet completion date 12 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Hirudinea 3 33 JT BVW PR 8 SP Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Coenagrionidae 2 22 BVW PR 9 CR Ephemeroptera (mayflies) Caenidae 1 11 JT BVW CG 6 SP Ephemerllidae 4 44 JT BVW CG 2 CR Heptageniidae 5 55 JT BVW SC 3 CG Isonychiidae 1 11 BVW CF 3 CG Plecoptera (stoneflies) Perlidae 14 154 JT BVW PR 2 CR Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Corydalidae 1 11 JT BVW PR 5 CR Coleoptera (beetles) Elmidae 24 264 I JT BVW SC 4 CG Dytiscidae 4 44 JT BVW PR 6 GN Psephenidae 3 33 JT BVW SC 4 CG Diptera (true flies) Tipulidae 3 33 JT BVW SH 4 BU Chironomidae 26 286 JT BVW CG 6 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
t
Other Total Individuals 91 1001 Total Taxa 13
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
u
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-1 STATION NAME Mouth LAT N 39deg 12749rsquo (upstream) LONG W 078deg 04209rsquo (upstream) LAT N 39deg 12729rsquo (downstream) LONG W 078deg 04148rsquo (downstream) FORM COMPILED BY Jeremy Tovar W S Bousquet RIVER BASIN Potomac River INVESTIGATORS Brandon Millholand JW Pangle Jeremy Tovar Heather Kepple
Collection date 10 October 2008 Sheet completion date 12 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Oligochaeta 3 35 JT BVW CG 8 BU Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Coenagrionidae 2 23 JT BVW PR 9 CR Ephemeroptera (mayflies) Heptageniidae 12 140 JT BVW SC 3 CG Isonychiidae 2 23 BVW CF 3 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Hydropsychidae 16 186 JT BVW CF 6 CG Philopotamidae 31 360 JT BVW CF 2 CG Hemiptera (true bugs) (Veliidae) (5) - JT BVW ( ) Megaloptera (dobsonflies etc) Corydalidae 10 116 JT BVW PR 5 CR Coleoptera (beetles) Dytiscidae 1 12 JT BVW PR 6 GN Psephenidae 7 81 JT BVW SC 4 CG Diptera (true flies) Tipulidae 2 23 BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
v
Other Total Individuals 86
(91) 999
Total Taxa 10 (11)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009 Veliidae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
w
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-2 STATION NAME Package Plant LAT N 39deg 12958rsquo (upstream) LONG W 078deg 04900 (upstream) LAT N 39deg 12958rsquo (downstream) LONG W 078deg 04822 (downstream) FORM COMPILED BY JW Pangle W Bousquet RIVER BASIN Potomac River INVESTIGATORS JW Pangle S Robertson J Tovar M Dahl B Millholland W Bousquet
Collection date 29 May 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Plecoptera (stoneflies) Perlidae 11 136 I JP BVW PR 1 CR Trichoptera (caddisflies) Hydropsychidae 10 123 I JP BVW CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 14 173 I JP BVW SC 4 CG Diptera (true flies) Chironomidae 32 395 I JP BVW CG 6 BU Simuliidae 14 173 I JP BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
x
Other Total Individuals 81 1000 Total Taxa 5
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-2 STATION NAME Package Plant LAT N 39deg 12958rsquo (upstream) LONG W 078deg 04900 (upstream) LAT N 39deg 12958rsquo (downstream) LONG W 078deg 04822 (downstream) FORM COMPILED BY JW Pangle W Bousquet RIVER BASIN Potomac River INVESTIGATORS JW Pangle Brandon Millholland Jeremy Tovar Heather Kepple
Collection date 10 October 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Heptageniidae 40 392 I WBJP SC 3 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Hydropsychidae 7 69 I JP WB BVW CF 6 CG Philopotamidae 28 275 I JP WB BVW CF 2 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Corydalidae 6 59 I JP WB BVW PR 5 CR Coleoptera (beetles) Psephenidae 7 69 I JP WB BVW SC 4 CG Elmidae 8 78 I JP WB BVW SC 4 CG Diptera (true flies) Tipulidae 6 59 I JP WB BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
y
z
Other Total Individuals 102 1001 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-3 STATION NAME Rt 664 Bridge LAT N 39deg 12934rsquo (upstream) LONG W 078deg 05019rsquo (upstream) LAT N 39deg 12951rsquo (downstream) LONG W 078deg 04958rsquo (downstream) FORM COMPILED BY S Robertson W Bousquet RIVER BASIN Potomac River INVESTIGATORS SRobertson BMillholland WBousquet MDahl JPangle
Collection date 28 May 2008 Sheet completion date 11 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Leptophlebiidae 1 15 I SGR BVW CG 2 CR Baetidae 1 15 I BVW CG 5 CG Plecoptera (stoneflies) Perlidae 3 46 I SGR BVW PR 1 CR Nemouridae 1 15 I BVW SH 3 CR Trichoptera (caddisflies) Hydropsychidae 5 77 I SGR BVW CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 27 415 I SGR BVW SC 4 CG Psephenidae 2 31 A SGR BVW SC 4 CG Diptera (true flies) Simuliidae 5 77 I SGR BVW CF 6 CG Chironomidae 20 308 I SGR BVW CG 6 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
aa
bb
Other Total Individuals 65 999 Total Taxa 9
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-3 STATION NAME Rt 664 Bridge LAT N 39deg 12934rsquo (upstream) LONG W 078deg 05019rsquo (upstream) LAT N 39deg 12951rsquo (downstream) LONG W 078deg 04958rsquo (downstream) FORM COMPILED BY S Robertson RIVER BASIN Potomac River INVESTIGATORS S Robertson M Dahl A Fisher H Kepple
Collection date 10 Oct 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Heptageniidae 2 20 I SGR SC 3 CG Isonychiidae 2 20 I BVW CF 2 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Philopotamidae 36 367 I SGR CF 2 CG Hydropsychidae 47 480 I SGR CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 2 20 A SGR SC 4 CG Psephenidae 8 82 A SGR SC 4 CG Diptera (true flies) Tabanidae 1 10 I SGR PR 7 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
cc
dd
Other Total Individuals 98 999 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-4 STATION NAME Unnamed Tributary LAT (upstream) N 39deg 13035rsquo LONG (upstream) W 078deg 05129rsquo LAT (downstream) N 39deg 13000rsquo LONG (downstream) W 078deg 5086rsquo FORM COMPILED BY A Fisher M Dahl rev W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson A Fisher H Kepple
Collection date 31 May 2008 Sheet completion date rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Flatworms (Turbellaria) Worms (Oligochaeta) Naididae 1 14 - BVW CG 8 BU Leeches (Hirudinea) Snails (Gastropoda) Clams (Bivalvia) Isopods (Isopoda) Asellidae 3 42 A AF BVW CG 8 SP Scuds (Amphipoda) Crayfish Shrimp (Decapoda) Water Mites (Hydracarina) Dragonflies (Odonata Anisoptera) Damselflies (Odonata Zygoptera) Mayflies (Ephemeroptera) Stoneflies (Plecoptera) Perlidae 2 28 I AF BVW PR 1 CR Caddisflies (Trichoptera) True Bugs (Hemiptera) Corixidae 1 14 I AF BVW MP 9 DV Dobsonflies (Megaloptera) Beetles (Coleoptera) Dytiscidae 1 14 I AF BVW PR 6 GN True Flies (Diptera) Chironomidae 61 847 I AF BVW CG 6 BU Simuliidae 3 42 I AF BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
ee
ff
Other Total Individuals 72 1001 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-4 STATION NAME Unnamed Tributary LAT (upstream) N 39deg 13035rsquo LONG (upstream) W 078deg 05129rsquo LAT (downstream) N 39deg 13000rsquo LONG (downstream) W 078deg 5086rsquo FORM COMPILED BY Andrew G M Fisher W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson A Fisher H Kepple
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Flatworms (Turbellaria) Worms (Oligochaeta) Lumbriculidae 2 32 A AF BVW CG 8 BU Leeches (Hirudinea) Snails (Gastropoda) Lymnaeidae 1 16 A BVW CG 9 GN Clams (Bivalvia) Isopods (Isopoda) Scuds (Amphipoda) Crayfish Shrimp (Decapoda) Cambaridae 1 16 A AF BVW GN 5 GN Water Mites (Hydracarina) Dragonflies (Odonata Anisoptera) Damselflies (Odonata Zygoptera) Calopterygidae 4 63 I BVW PR 7 CL Mayflies (Ephemeroptera) Tricorythidae 1 16 I BVW CG 5 SP Stoneflies (Plecoptera) Caddisflies (Trichoptera) Phryganeidae 4 63 I AF BVW SH 4 CR Hydropsychidae 6 95 I AF BVW CF 6 CG I True Bugs (Hemiptera) Corixidae 8 127 I AF BVW MP 9 DV (Veliidae ) (7) - I AF BVW ( ) Dobsonflies (Megaloptera) Sialidae 3 48 I AF BVW PR 7 SP Corydalidae 2 32 I AF BVW PR 5 CR Beetles (Coleoptera) Haliplidae 1 16 A AF BVW MP 8 GN Dysticidae 7 111 I AF BVW PR 6 GN Hydrophilidae 1 16 A AF BVW PR 8 GN Elmidae 3 48 I BVW SC 4 CG True Flies (Diptera) Chironomidae 8 127 I AF BVW CG 6 BU Simuliidae 7 111 I AF BVW CF 6 CG Tipulidae 4 63 I AF BVW SH 4 GU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
gg
hh
Other Total Individuals 63
(70) 1000
Total Taxa 17 (18)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Veliidae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-5 STATION NAME Lick Run LAT N 39deg 12752rsquo (upstream) LONG W 078deg 05685rsquo (upstream) LAT N 39deg 12784rsquo (downstream) LONG W 078deg 05631rsquo (downstream) FORM COMPILED BY Brandon Millholland W Bousquet RIVER BASIN Potomac River INVESTIGATORS J Pangle S Robertson W Bousquet JTovar M Dahl
Collection date 29 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Gomphidae 1 15 I BVW PR 3 BU Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Leptophlebiidae 11 167 A LBM BVW CG 2 CR Plecoptera (stoneflies) Perlidae 10 152 A LBM BVW PR 1 CR Perlodiae 1 15 A LBM BVW PR 2 CR Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 5 76 A LBM BVW SC 4 CG Elmidae 2 30 I LBM BVW SC 4 CG Diptera (true flies) (Culicidae) (3) - P LMB BVW ( ) Chironomidae 23 348 I LBM BVW CG 6 BU Simuliidae 13 197 I LBM BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
ii
jj
Other Total Individuals 66
(69) 1000
Total Taxa 7 (8)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Culicidae (Diptera) pupae not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl J Pangle S Robertson W Bousquet B Millholland J Tovar H Kepple
Collection date 30 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 1 12 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Asellidae 114 965 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Plecoptera (stoneflies) Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 2 23 A MRD BVW SC 4 CG Diptera (true flies)
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
kk
ll
Other Total Individuals 117 1000 Total Taxa 3
Notes Subsample taken 29 October 2009 All taxonomic determinations by MRD were confirmed by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson K McCann A Fisher H Kepple A Barrett
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 2 17 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Sphaeriidae 2 17 I MRD BVW CF 8 BU Isopoda (isopods) Asellidae 99 853 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Calopterygidae 2 17 A MRD BVW PR 7 CL Ephemeroptera (mayflies) Leptophlebiidae 2 17 I MRD BVW CG 2 CR Heptageniidae 4 34 A MRD BVW SC 3 CG Plecoptera (stoneflies) Perlidae 1 09 A MRD BVW PR 1 CR Trichoptera (caddisflies) Hemiptera (true bugs) (Gerridae) (1) - A MRD BVW ( ) Corixidae 1 09 A MRD BVW PR 5 CR Megaloptera (dobsonflies etc) Coleoptera (beetles) Dryopidae 1 09 A MRD BVW SC 5 CG Diptera (true flies) Tipulidae 2 17 I BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
mm
nn
Other Total Individuals 116
(117) 999
Total Taxa 10 (11)
Notes Subsample taken 6 November 2008 Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009 Gerridae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
- Disclaimer ii
- Title Page iii
- Acknowledgements v
- Table of Contents vii
- SECTION 2 RESULTS DATA TABLES 7
- OVERVIEW AND FINDINGS
- Introduction
- RESULTS DATA TABLES
- REFERENCES
- FIELD DATA SHEETS
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- SubstrateType
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- WATER QUALITY ANALYSIS
- THROUGH THE VIRGINIA
- STREAM CONDITION INDEX (VSCI)
- LABORATORY BENCH SHEETS
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
Site HLR-2 Package Plant
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 5 189 2642 EPT taxa 2 117 1713 Ephemeroptera 00 477 004 Plec +Tric less Hydropsych 110 562 1965 Scrapers 173 467 3706 Chironomidae 395 16 6157 Top 2 Dominant 568 328 6438 Hilsenhoff Biotic Index (HBI) 50 29 704VSCI Score 370
According to the DEQrsquos water quality standards aquatic life at this site is severely stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 4 VSCI Scores for Package Plant (HLR-2) Site in Hiatt-Lick Run Sampled 29 May 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 7 189 3702 EPT taxa 34 117 2563 Ephemeroptera 392 477 8214 Plec +Tric less Hydropsych 275 562 4895 Scrapers 539 467 10006 Chironomidae 00 16 10007 Top 2 Dominant 667 328 4968 Hilsenhoff Biotic Index (HBI) 32 29 958VSCI Score 674
According to the DEQrsquos water quality standards aquatic life at this site is good See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 5 VSCI Scores for Package Plant (HLR-2) Site in Hiatt-Lick Run Sampled 10 October 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
8
Site HLR-3 Route 664 Bridge
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 9 189 4762 EPT taxa 5 117 4273 Ephemeroptera 30 477 634 Plec +Tric less Hydropsych 61 562 1095 Scrapers 446 467 9556 Chironomidae 308 16 7037 Top 2 Dominant 723 328 4128 Hilsenhoff Biotic Index (HBI) 48 29 732VSCI Score 485
According to the DEQrsquos water quality standards aquatic life at this site is moderately stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 6 VSCI Scores for Route 664 Bridge (HLR-3) Site in Hiatt-Lick Run Sampled 28 May 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 7 189 3702 EPT taxa 4 117 3423 Ephemeroptera 40 477 844 Plec +Tric less Hydropsych 367 562 6535 Scrapers 122 467 2616 Chironomidae 00 16 10007 Top 2 Dominant 847 328 2288 Hilsenhoff Biotic Index (HBI) 42 29 817VSCI Score 469
According to the DEQrsquos water quality standards aquatic life at this site is moderately stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 7 VSCI Scores for Route 664 Bridge (HLR-3) Site in Hiatt-Lick Run Sampled 10 October 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
9
Site HLR-4 Unnamed Tributary
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 7 189 3702 EPT taxa 1 117 853 Ephemeroptera 00 477 004 Plec +Tric less Hydropsych 28 562 495 Scrapers 00 467 006 Chironomidae 847 16 1557 Top 2 Dominant 889 328 1658 Hilsenhoff Biotic Index (HBI) 60 29 563VSCI Score 173
According to the DEQrsquos water quality standards aquatic life at this site is severely stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 8 VSCI Scores for Unnamed Tributary (HLR-4) Site in Hiatt-Lick Run Sampled 31 May 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 17 189 8992 EPT taxa 3 117 2563 Ephemeroptera 16 477 344 Plec +Tric less Hydropsych 63 562 1125 Scrapers 48 467 1036 Chironomidae 127 16 8877 Top 2 Dominant 254 328 10008 Hilsenhoff Biotic Index (HBI) 63 29 521VSCI Score 476
According to the DEQrsquos water quality standards aquatic life at this site is moderately stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 9 VSCI Scores for Unnamed Tributary (HLR-4) Site in Hiatt-Lick Run Sampled 10 October 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
10
Site HLR-5 Lick Run
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 7 189 3702 EPT taxa 3 117 2563 Ephemeroptera 167 477 3504 Plec +Tric less Hydropsych 167 562 2975 Scrapers 106 467 2276 Chironomidae 348 16 6637 Top 2 Dominant 545 328 6778 Hilsenhoff Biotic Index (HBI) 43 29 803VSCI Score 455
According to the DEQrsquos water quality standards aquatic life at this site is moderately stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 10 VSCI Scores for Lick Run (HLR-5) Site in Hiatt-Lick Run Sampled 29 May 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
Note
Water conditions at the Lick Run (HLR-5) site were too low in October 2008 to permit a sample to be taken
11
Site HLR-6 McCann Farm
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 3 189 1592 EPT taxa 0 117 003 Ephemeroptera 00 477 004 Plec +Tric less Hydropsych 00 562 005 Scrapers 23 467 496 Chironomidae 00 16 10007 Top 2 Dominant 988 328 188 Hilsenhoff Biotic Index (HBI) 79 29 296VSCI Score 190
According to the DEQrsquos water quality standards aquatic life at this site is severely stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 11 VSCI Scores for McCann Farm (HLR-6) Site in Hiatt-Lick Run Sampled 30 May 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 10 189 5292 EPT taxa 3 117 2563 Ephemeroptera 51 477 1074 Plec +Tric less Hydropsych 09 562 165 Scrapers 43 467 926 Chironomidae 00 16 10007 Top 2 Dominant 887 328 1688 Hilsenhoff Biotic Index (HBI) 75 29 352VSCI Score 315
According to the DEQrsquos water quality standards aquatic life at this site is severely stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 12 VSCI Scores for McCann Farm (HLR-6) Site in Hiatt-Lick Run Sampled 10 October 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
12
REFERENCES
Barbour Michael T Jeroen Gerritsen Blaine D Snyder and James B Stribling 1999 Rapid bioassessment protocols for use in streams and wadeable rivers periphyton benthic macroinvertebrates and fish Second edition EPA 841-B-99-002 Washington DC US Environmental Protection Agency Office of Water Available online at httpwwwepagovOWOWmonitoringbioasspshtml Burton June and Jeroen Gerritsen 2003 A stream condition index for Virginia non-coastal streams Prepared for the US EPA Office of Science and Technology US EPA Region 3 Environmental Services Division and Virginia Department of Environmental Quality Owings Mills Maryland Tetra Tech Inc Available online at httpwwwdeqvirginiagovwatermonitoringpdf vastrmconpdf Hessler Rachel A Andrea R Martin Heidi D Ruggeri William B Seymour Jr and Woodward S Bousquet 2006 Water quality assessment using benthic macroinvertebrates A study and compilation Winchester Virginia Environmental Studies Program Shenandoah University Occasional publication no 10 Igoe Robert 2006 DEQ identifies nine impaired streams in Clarke Frederick The Winchester Star 15 July B1 Jackson Charlie 2005 Survey targets Opequon cleanup The Winchester Star 19 August B1 Johnston Elizabeth A and Woodward S Bousquet 2003 What happens when it rains A study of water quality in Abrams Creek and Town Run Winchester and Frederick County Virginia Winchester Virginia Environmental Studies Program Shenandoah University Occasional publication no7 Merritt Richard W and Kenneth W Cummins 1996 An introduction to the aquatic insects of North America Dubuque Iowa KendallHunt Publishing Company Opequon Creek IP Steering Committee Virginia Tech Department of Biological Systems Engineering and Center for TMDL and Watershed Studies University of Virginia Institute for Environmental Negotiation and West Virginia University Division of Resource Management 2006 Opequon Creek watershed TMDL implementation plan Blacksburg Virginia Virginia Tech Department of Biological Systems Engineering Available online at httpwwwtmdlbsevteduoutreachC82 Pennak Robert W 1953 Fresh-water invertebrates of the United States New York New York The Ronald Press Company Rosenburg David M and Vincent H Resh 1993 Freshwater biomonitoring and benthic macroinvertebrates New York New York Chapman amp Hall Shenandoah University Public Relations Office 2002 Data collected by SU and its partners helps identify impaired water Winchester Virginia Available online at httpwwwsuedutemp_ newscfmurlnum=32 Thorp James H and Alan P Covich 1991 Ecology and classification of North American freshwater invertebrates San Diego California Academic Press Inc
13
US Environmental Protection Agency 1997 Volunteer stream monitoring a methods manual EPA 841-B-97-003 Washington DC US Environmental Protection Agency Office of Water Van Meter Val 2003 Opequon Creek pollution limits to be established The Winchester Star 4 April B1 Virginia Department of Environmental Quality 2006a Virginia 305(b)303(d) water quality integrated report to Congress and the EPA Administrator for the period January 1 2000 to December 31 2004 Richmond Virginia Virginia Department of Environmental Quality Available online at httpwwwdeqvirginiagovwqair2006html __________ 2006b Using probabilistic data to validate the non-coastal Virginia Stream Condition Index (draft) VDEQ technical bulletin WQA2006-001 Virginia Department of Environmental Quality Available online at httpwwwloudounwatershedorgstream_assessmentreference_documents VA_DEQ_SCI_Validationpdf __________ 2008 Biological monitoring program quality assurance project plan for wadeable streams and rivers Richmond Virginia Virginia Department of Environmental Quality Update available online at httpwwwdeqstatevausbiomonitoringdocumentsBioMonQAPP_13Aug2008pdf Virginia Tech Aquatic Entomology Laboratory 2005 Taxa dictionary [Excel file] Blacksburg Virginia Virginia Tech Department of Biological Systems Engineering 2003a Opequon watershed TMDLs for benthic impairments Abrams Creek and Lower Opequon Creek Frederick and Clarke Counties Virginia Prepared for the Virginia Department of Environmental Quality and Virginia Department of Conservation and Recreation Blacksburg Virginia Available online at httpwwwdeqstate vaustmdlapptmdlsshenrvrabropebcpdf Voshell J Reese Jr 2002 A guide to common freshwater invertebrates of North America Blacksburg Virginia The McDonald amp Woodward Publishing Company
14
APPENDICES
15
16
a
Appendix A
FIELD DATA SHEETS
This appendix presents a Site Characterization Field Data Sheet for each of the six sampling reaches established on Hiatt Run and Lick Run for this study They were filled out during the sampling runs made in May 2008
These forms were adapted by Shenandoah University from forms used by the EPA and Virginia DEQ for recording characteristics of sampling sites
b
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Mouth STATION _HLR-1_____ STREAM CLASS LAT N 39 12749 (upstream) LONG W 078 04209(upstream) LAT N 39 12729 (downstream) LONG W 078 04148 (downstream) FORM COMPILED BY Jeremy Tovar RIVER BASIN Potomac River INVESTIGATORS JPangle B Millholland MDahl SRobertson W Bousquet JTovar BBousquet
DATE May 21 2008_________ TIME_250 PM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature __24___degC showers (intermittent) Comments _______________________
___ cloud cover _0_ ______________________________
X clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x ( Significant springs andor seeps) x (Valley ) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS
c
d
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence x FieldPasture Industrial Obvious sources Agricultural Other ____________ Stream scour steep slope erosion x Residential Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left Field and young thin deciduous forest 100 Bank=Grass cobble and shrub Right 90 maturing deciduous forest 45 shale slope Bank=Bedrock soil and herbs Indicate the dominant vegetation type(s) and record the dominant species
x Trees Shrubs x (Grasses) Forbs Dominant species Oak species and mix deciduous
INSTREAM FEATURES
Measured reach length ___100__m Canopy Cover Estimated stream width _35__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area __350_m2
Estimated stream depth _008_m Proportion of Reach Represented by Stream High water mark _15_m Morphology Types Surface velocity (at thalweg) _05__msec x Riffle__75___ x Run__20___ x Pool__5___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___00__ m2 Density of LWD _-_ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __15_____ x Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present Algae
WATER QUALITY Temperature __19___degC Water Odors x NormalNone Sewage Specific Conductance - Petroleum Chemical Fishy Other___________________ Dissolved Oxygen - Water Surface Oils Slick Sheen Globs Flecks pH __-_ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used _____NA________ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic x None Relict shells Other______None_____ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 8 Detritus sticks wood coarse plant materials
0 Boulder gt256 mm (10rsquorsquo) 0
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 51 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 40 Sand 006-2 mm (gritty) 1 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University
SITE CHARACTERIZATION FIELD DATA SHEET FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Package Plant STATION HLR-2 STREAM CLASS LAT N 39 129580 (upstream) LONG W 078 04900 (upstream) LAT N 39 129581 (downstream) LONG W 078 04822 (downstream) FORM COMPILED BY JW Pangle RIVER BASIN Potomac River INVESTIGATORS JPangle JTovar MDahl B Millholland SRobertson W Bousquet
DATE__19 May 2008_ TIME__1240__ PM
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature _____degC showers (intermittent) Comments __Sunny Day_____________
_5__ cloud cover _0__ ______________________________
x clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x (Significant springs andor seeps) x (Valley) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS
e
f
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence FieldPasture Industrial Obvious sources Agricultural Other ____________ __Upstream Construction and Stream bank Scour Residential __________________________________ Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left Deciduous forest with thick herb and shrub layers bank= vegetation and rocks Right Deciduous forest with thick herb and shrub layers bank=vegetation soil and rocks Indicate the dominant vegetation type(s) and record the dominant species
x Trees x Shrubs Grasses Forbs Dominant species Black Walnut White Ash Hackberry and American Sycamore
INSTREAM FEATURES
Measured reach length 100 m Canopy Cover Estimated stream width _35__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area __350_m2
Estimated stream depth __15__m Proportion of Reach Represented by Stream High water mark __22___m Morphology Types Surface velocity (at thalweg) _035__msec x Riffle__30___ x Run__30___ x Pool__40___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___10____ m2 Density of LWD _______ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __30___ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present__________________________________________________________
WATER QUALITY Temperature __18___degC Water Odors x NormalNone Sewage Specific Conductance __-____ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ____-____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used _____NA____ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other___None_____ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach SubstrateType Characteristic
Composition in Sampling Reach
Bedrock 3 Detritus sticks wood coarse plant materials
2 Boulder gt256 mm (10rsquorsquo) 5
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 70 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 22 Sand 006-2 mm (gritty) 0 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiattrsquos Run LOCATION Rt 664 Bridge STATION HLR-3 STREAM CLASS LAT N 39 12934 (upstream) LONG W 078 05019 (upstream) LAT N 39 12958 (downstream) LONG W 078 04958 (downstream) FORM COMPILED BY Sean Robertson RIVER BASIN Potomac River INVESTIGATORS SRobertson BMillholland WBousquet MDahl JPangle
DATE 28 May 2008 TIME 1127 AM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality 2008
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature __14___degC showers (intermittent) x Trace Comments _______________________
_65 x cloud cover ___ ______________________________
x clearsunny __________________________________
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x (Significant springs andor seeps) x (Valley) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS
g
h
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence Some evidence x FieldPasture Industrial x Obvious sources Agricultural x Other Old Package Plant Downstream x Residential Bridge and Road Power line and Pump station Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left 70 field 30 shrubs and deciduous trees Bank=Soil and vegetation Right Maturing deciduous forest steep slope Bank=Shale Bedrock Indicate the dominant vegetation type(s) and record the dominant species
x Trees x Shrubs x Grasses Forbs Dominant species Sycamore and Red Oaks
INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width __51___m Fully open x Partly open Partly shaded Fully shaded Sampling reach area _510_m2
Estimated stream depth __135___m Proportion of Reach Represented by Stream High water mark __-___m Morphology Types Surface velocity (at thalweg) __20___msec x Riffle_40_ x Run_50_ x Pool 10 Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___0____ m2 Density of LWD ___-____ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation ___50____ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present Brown Algae
WATER QUALITY Temperature __17___degC Water Odors x NormalNone Sewage Specific Conductance ___-___ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ___-_____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used ____NA_______ Clear x Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other____None____ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 15 Detritus sticks wood coarse plant materials
0 Boulder gt256 mm (10rsquorsquo) 15
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 62 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 8 Sand 006-2 mm (gritty) 0 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Unnamed Tributary STATION HLR-4 STREAM CLASS LAT N 39 13035 (upstream) LONG W 078 05129 (upstream) LAT N 39 13000 (downstream) LONG W 078 05086 (downstream) FORM COMPILED BY Marie Dahl RIVER BASIN Potomac River INVESTIGATORS M Dahl HKepple SRobertson AFisher
DATE 31 May 2008 TIME 5 PM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality 2008
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) YES x NO
rain (steady rain) Air Temperature __22___degC showers (intermittent) Comments _______________________
_0__ cloud cover _0__ ______________________________
x clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area __-___ km2 mi2 Montane Significant springs andor seeps x Valley Marsh or swamp Montane amp Valley Mixture of origins Other ___________________
COMMENTS Snowden Bridge development (upstream) and sewer line
i
j
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence x FieldPasture Industrial Obvious sources Agricultural Other ____________ Construction upstream and adjacent x Residential Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left Forested with rocky banks field across silt fence and 2m tree strip Right Forested with some rocky banks and forest through the highway Indicate the dominant vegetation type(s) and record the dominant species
x Trees x Shrubs x Grasses Forbs Dominant species White Ash and Hackberry
INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width ___38__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area __380__m2
Estimated stream depth __12__cm Proportion of Reach Represented by Stream High water mark __2___m Morphology Types Surface velocity (at thalweg) __46___msec x Riffle__25___ x Run__70___ x Pool__5___ Channelized Yes x No Dam Present x Yes No
LARGE WOODY DEBRIS
LWD _______ m2 Density of LWD _______ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation ___NA____ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present___Algae___
WATER QUALITY Temperature __16___degC Water Odors x NormalNone Sewage Specific Conductance __-____ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ___-_____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used ____NA_____ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells x Other___Shale_______ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 0 Detritus sticks wood coarse plant materials
10 Boulder gt256 mm (10rsquorsquo) 5
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 45 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 30 Sand 006-2 mm (gritty) 0 Marl grey shell
fragments 0
Silt 0004-006 mm 20 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Lick Run STATION HLR-5 STREAM CLASS LAT N 39 127517 (upstream) LONG W 078 05685(upstream) LAT N 39 12784 (downstream) LONG W 078 05631 (downstream) FORM COMPILED BY B Millholland RIVER BASIN Potomac River INVESTIGATORS B Millholland JTovar SRobertson MDahl WBousquet JPangle
DATE 29 May 2008 TIME 1040 AM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project
k
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature __15___degC showers (intermittent) Comments _______________________
_5_ X cloud cover _0__ ______________________________
x clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane Significant springs andor seeps x Valley Marsh or swamp Montane amp Valley Mixture of origins Other ___________________
COMMENTS Lick Run appears to be intermittent and may be dry in Fall 2008
l
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence x FieldPasture Industrial Obvious sources Agricultural Other ____________ Adjacent logging stream bank scour Residential Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left 85 young deciduous forest 15 logged Bank=Soil vegetation and cobbles Right Young deciduous forest on 30 slope Bank= Soil vegetation and cobble Indicate the dominant vegetation type(s) and record the dominant species
x Trees Shrubs Grasses Forbs Dominant species Mixed Deciduous INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width __11__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area 110 m2
Estimated stream depth _009__m Proportion of Reach Represented by Stream High water mark __96___m Morphology Types Surface velocity (at thalweg) __01___msec x Riffle__30___ x Run__5___ x Pool__65___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___0____ m2 Density of LWD ___-____ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __70___ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present__________________________________________________________
WATER QUALITY Temperature __14___degC Water Odors x NormalNone Sewage Specific Conductance __-__ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ___-___ Water Surface Oils Slick Sheen Globs Flecks pH __-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used _____NA______ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other_____None_____
SEDIMENT AND SUBSTRATE
Odors Deposits Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other________________ x Other Slightly Salty Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 0 Detritus sticks wood coarse plant materials
5 Boulder gt256 mm (10rsquorsquo) 0
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 75 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 23 Sand 006-2 mm (gritty) 2 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION McCann Farm STATION HLR-6 STREAM CLASS LAT N 39 13076 (upstream) LONG W 078 06539 (upstream) LAT N 39 13063 (downstream) LONG W 078 06473 (downstream) FORM COMPILED BY MDahl RIVER BASIN Potomac River INVESTIGATORS MDahl JPangle SRobertson W Bousqet BMillholland JTovar H Kepple
DATE 30 May 2008 TIME 1050 AM (start)
PROJECT NAME
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) YES x NO
rain (steady rain) Air Temperature __18___degC showers (intermittent) Comments _______________________
_85_x cloud cover _10__ ______________________________
clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x (Significant springs andor seeps) x ( Valley) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS Multi-habitat sampling with D-frame nets and seine
m
n
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial x No evidence Some evidence x FieldPasture Industrial Obvious sources x Agricultural Other ____________ Minor stream bank scour Residential Local Watershed Erosion x None Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left 35 bank grasses scattered trees and some bushes Right High bank with grasses and scattered trees Indicate the dominant vegetation type(s) and record the dominant species
x Trees Shrubs Grasses Forbs Dominant species Black Walnut and Festuca elatior
INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width __2___m Fully open Partly open x Partly shaded Fully shaded Sampling reach area __200_m2
Estimated stream depth __03_m Proportion of Reach Represented by Stream High water mark __1___m Morphology Types Surface velocity (at thalweg) __166_msec x Riffle__0___ x Run__55___ x Pool__45___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___2____ m2 Density of LWD ___-____ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __20_____ x Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present ____Glyceria (grass)sp__________
WATER QUALITY Temperature __16___degC Water Odors x NormalNone Sewage Specific Conductance ___-___ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ____-____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used ______NA___ Clear x Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other__NONE__ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 0 Detritus sticks wood coarse plant materials
5 Boulder gt256 mm (10rsquorsquo) 0
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 27 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 38 Sand 006-2 mm (gritty) 5 Marl grey shell
fragments 0 (lt1)
Silt 0004-006 mm 25 Clay lt 0004 mm (slick) 5 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Appendix B
WATER QUALITY ANALYSIS THROUGH THE VIRGINIA
STREAM CONDITION INDEX (VSCI)
The Virginia Stream Condition Index (VSCI) relies on eight metrics to categorize stream health According to Burton and Gerritsen (2003 p 1-1) this index ldquohellip can be used as a primary indicator of ecosystem health and can identify impairment with respect to the reference (or natural) conditionrdquo These metrics are numeric measures of a biological communityrsquos species composition diversity ecological niches or tolerance to pollution
Table 13 on the next page describes each VSCI metric lists its formula and provides the best standard value (BSV) for that metric Based on a 100-point scale the BSV is the score that reflects optimum conditions for aquatic life ie the best water quality value possible (DEQ 2006b pp 8 33) The table also explains whether the score increases or decreases with environmental stress
As Table 13 indicates each of the eight VSCI metrics is calculated individually Each formula provides a unitless score based on a 100-point scale with 0 as the worst possible score and 100 as the best Any metric that provides a result above 100 is changed to 100 in order for the index to work properly After the eight metrics are calculated they are summed and averaged to provide a final VSCI score on a 100-point scale
Based on statistical analysis of Virginiarsquos streams and their BMI communities the DEQ (2006b p 8) currently uses the following tiers to interpret VSCI scores
VSCI Scores Aquatic Life Use
(ALI) Tiers
lt 42 severely stressed
42-55 moderately stressed
55-63 unknownfair (gray zone)
63-73 good
gt73 excellent
Section 2 of this report presents the results of calculations for the six BMI sampling sites the research team studied in the Hiatt-Lick Run subwatershed in 2008
o
p
1 Total taxa the number of distinct taxa in the sample measures the overall diversity of the macroinvertebrate assemblage The best standard value (BSV) is 189 Score = 100 x (X189) Total taxa decreases with stress 2 EPT taxa the sum of the distinct taxa in the orders Ephemeroptera (mayflies) Plecoptera (stoneflies) and Trichoptera (caddisflies) These orders are known to be sensitive to pollution The best standard value (BSV) is 117 Score = 100 x (X117) EPT taxa decreases with stress 3 Percent Ephemeroptera the percent abundance of individuals in the sample that belong to the order Ephemeroptera (mayflies) The best standard value (BSV) is 477 Score = 100 x (X477) Percent Ephemeroptera decreases with stress 4 Percent Plecoptera + Trichoptera less Hydropsychidae the percent abundance of individuals in the sample that belong to the orders Plecoptera (stoneflies) and Trichoptera (caddisflies) excluding the pollution-tolerant caddisfly family Hydropsychidae The best standard value (BSV) is 562 Score = 100 x (X562) Percent Plecoptera + Trichoptera less Hydropsychidae decreases with stress 5 Percent Scrapers the percent abundance of individuals in the sample whose primary functional mechanism for feeding is grazing on the substrate or periphyton (ie attached algae and associated material) The best standard value (BSV) is 467 Score = 100 x (X467) Percent scrapers decreases with stress 6 Percent Chironomidae the percent abundance of individuals in the sample that are Chironomidae (non-biting midge) larvae and pupae The best standard value (BSV) is 16 Score = 100 x [(100-X)(100-16)] Percent Chironomidae increases with stress 7 Percent Top Two Dominant the percent abundance of the two most frequent taxa present in the sample The best standard value (BSV) is 328 Score = 100 x [(100-X)(100-328)] The Percent Top 2 Dominant increases with stress 8 Hilsenhoff Biotic Index (HBI) an abundance-weighted average pollution tolerance of all the individuals within a sample keyed to the family level Tolerance ratings range from 1 (highly intolerant) to 10 (highly tolerant) The best standard value (BSV) is 29 Score = 100 x [(10-X)(10-29)] The Hilsenhoff Biotic Index (HBI) increases with stress ______________________________________________________________________________ Table 13 Description of Benthic Macroinvertebrate VSCI Metrics Includes responses (increases decreases) of each VSCI metric to degraded water quality conditions Adapted from Burton and Gerritsen (2003 pp 3-15 3-16 6-2) and DEQ (2008)
q
Appendix C
LABORATORY BENCH SHEETS
Shenandoah Universityrsquos Environmental Studies Department developed the Benthic Macroinvertebrate Laboratory Bench Sheet and VSCI Worksheet to record BMI specimen identifications and their VSCI classifications for each sample
This form is based in part on bench sheets developed by the EPA and Virginia DEQ for recording specimen identifications Additional columns on Shenandoah Universityrsquos forms for relative numbers and VSCI classifications make the calculation and verification of VSCI scores easier
Note the column for taxonomistrsquos initials Specimens were identified by students in Shenandoah Universityrsquos research group and then verified or where necessary changed by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality This external review helps assure the validity of the study Originals of these bench sheets with handwritten taxonomistsrsquo initials will be kept on file at Shenandoah Universityrsquos Environmental Studies Department for five years after the completion of this study
r
s
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-1 STATION NAME Mouth LAT N 39deg 12749rsquo (upstream) LONG W 078deg 04209rsquo (upstream) LAT N 39deg 12729rsquo (downstream) LONG W 078deg 04148rsquo (downstream) FORM COMPILED BY Jeremy Tovar W S Bousquet RIVER BASIN Potomac River INVESTIGATORS Marie Dahl Brandon Millholand J W Pangle Sean Robertson Jeremy Tovar Woodward Bousquet Ben Bousquet
Collection date 21 May 2008 Sheet completion date 12 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Hirudinea 3 33 JT BVW PR 8 SP Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Coenagrionidae 2 22 BVW PR 9 CR Ephemeroptera (mayflies) Caenidae 1 11 JT BVW CG 6 SP Ephemerllidae 4 44 JT BVW CG 2 CR Heptageniidae 5 55 JT BVW SC 3 CG Isonychiidae 1 11 BVW CF 3 CG Plecoptera (stoneflies) Perlidae 14 154 JT BVW PR 2 CR Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Corydalidae 1 11 JT BVW PR 5 CR Coleoptera (beetles) Elmidae 24 264 I JT BVW SC 4 CG Dytiscidae 4 44 JT BVW PR 6 GN Psephenidae 3 33 JT BVW SC 4 CG Diptera (true flies) Tipulidae 3 33 JT BVW SH 4 BU Chironomidae 26 286 JT BVW CG 6 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
t
Other Total Individuals 91 1001 Total Taxa 13
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
u
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-1 STATION NAME Mouth LAT N 39deg 12749rsquo (upstream) LONG W 078deg 04209rsquo (upstream) LAT N 39deg 12729rsquo (downstream) LONG W 078deg 04148rsquo (downstream) FORM COMPILED BY Jeremy Tovar W S Bousquet RIVER BASIN Potomac River INVESTIGATORS Brandon Millholand JW Pangle Jeremy Tovar Heather Kepple
Collection date 10 October 2008 Sheet completion date 12 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Oligochaeta 3 35 JT BVW CG 8 BU Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Coenagrionidae 2 23 JT BVW PR 9 CR Ephemeroptera (mayflies) Heptageniidae 12 140 JT BVW SC 3 CG Isonychiidae 2 23 BVW CF 3 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Hydropsychidae 16 186 JT BVW CF 6 CG Philopotamidae 31 360 JT BVW CF 2 CG Hemiptera (true bugs) (Veliidae) (5) - JT BVW ( ) Megaloptera (dobsonflies etc) Corydalidae 10 116 JT BVW PR 5 CR Coleoptera (beetles) Dytiscidae 1 12 JT BVW PR 6 GN Psephenidae 7 81 JT BVW SC 4 CG Diptera (true flies) Tipulidae 2 23 BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
v
Other Total Individuals 86
(91) 999
Total Taxa 10 (11)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009 Veliidae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
w
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-2 STATION NAME Package Plant LAT N 39deg 12958rsquo (upstream) LONG W 078deg 04900 (upstream) LAT N 39deg 12958rsquo (downstream) LONG W 078deg 04822 (downstream) FORM COMPILED BY JW Pangle W Bousquet RIVER BASIN Potomac River INVESTIGATORS JW Pangle S Robertson J Tovar M Dahl B Millholland W Bousquet
Collection date 29 May 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Plecoptera (stoneflies) Perlidae 11 136 I JP BVW PR 1 CR Trichoptera (caddisflies) Hydropsychidae 10 123 I JP BVW CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 14 173 I JP BVW SC 4 CG Diptera (true flies) Chironomidae 32 395 I JP BVW CG 6 BU Simuliidae 14 173 I JP BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
x
Other Total Individuals 81 1000 Total Taxa 5
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-2 STATION NAME Package Plant LAT N 39deg 12958rsquo (upstream) LONG W 078deg 04900 (upstream) LAT N 39deg 12958rsquo (downstream) LONG W 078deg 04822 (downstream) FORM COMPILED BY JW Pangle W Bousquet RIVER BASIN Potomac River INVESTIGATORS JW Pangle Brandon Millholland Jeremy Tovar Heather Kepple
Collection date 10 October 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Heptageniidae 40 392 I WBJP SC 3 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Hydropsychidae 7 69 I JP WB BVW CF 6 CG Philopotamidae 28 275 I JP WB BVW CF 2 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Corydalidae 6 59 I JP WB BVW PR 5 CR Coleoptera (beetles) Psephenidae 7 69 I JP WB BVW SC 4 CG Elmidae 8 78 I JP WB BVW SC 4 CG Diptera (true flies) Tipulidae 6 59 I JP WB BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
y
z
Other Total Individuals 102 1001 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-3 STATION NAME Rt 664 Bridge LAT N 39deg 12934rsquo (upstream) LONG W 078deg 05019rsquo (upstream) LAT N 39deg 12951rsquo (downstream) LONG W 078deg 04958rsquo (downstream) FORM COMPILED BY S Robertson W Bousquet RIVER BASIN Potomac River INVESTIGATORS SRobertson BMillholland WBousquet MDahl JPangle
Collection date 28 May 2008 Sheet completion date 11 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Leptophlebiidae 1 15 I SGR BVW CG 2 CR Baetidae 1 15 I BVW CG 5 CG Plecoptera (stoneflies) Perlidae 3 46 I SGR BVW PR 1 CR Nemouridae 1 15 I BVW SH 3 CR Trichoptera (caddisflies) Hydropsychidae 5 77 I SGR BVW CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 27 415 I SGR BVW SC 4 CG Psephenidae 2 31 A SGR BVW SC 4 CG Diptera (true flies) Simuliidae 5 77 I SGR BVW CF 6 CG Chironomidae 20 308 I SGR BVW CG 6 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
aa
bb
Other Total Individuals 65 999 Total Taxa 9
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-3 STATION NAME Rt 664 Bridge LAT N 39deg 12934rsquo (upstream) LONG W 078deg 05019rsquo (upstream) LAT N 39deg 12951rsquo (downstream) LONG W 078deg 04958rsquo (downstream) FORM COMPILED BY S Robertson RIVER BASIN Potomac River INVESTIGATORS S Robertson M Dahl A Fisher H Kepple
Collection date 10 Oct 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Heptageniidae 2 20 I SGR SC 3 CG Isonychiidae 2 20 I BVW CF 2 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Philopotamidae 36 367 I SGR CF 2 CG Hydropsychidae 47 480 I SGR CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 2 20 A SGR SC 4 CG Psephenidae 8 82 A SGR SC 4 CG Diptera (true flies) Tabanidae 1 10 I SGR PR 7 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
cc
dd
Other Total Individuals 98 999 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-4 STATION NAME Unnamed Tributary LAT (upstream) N 39deg 13035rsquo LONG (upstream) W 078deg 05129rsquo LAT (downstream) N 39deg 13000rsquo LONG (downstream) W 078deg 5086rsquo FORM COMPILED BY A Fisher M Dahl rev W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson A Fisher H Kepple
Collection date 31 May 2008 Sheet completion date rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Flatworms (Turbellaria) Worms (Oligochaeta) Naididae 1 14 - BVW CG 8 BU Leeches (Hirudinea) Snails (Gastropoda) Clams (Bivalvia) Isopods (Isopoda) Asellidae 3 42 A AF BVW CG 8 SP Scuds (Amphipoda) Crayfish Shrimp (Decapoda) Water Mites (Hydracarina) Dragonflies (Odonata Anisoptera) Damselflies (Odonata Zygoptera) Mayflies (Ephemeroptera) Stoneflies (Plecoptera) Perlidae 2 28 I AF BVW PR 1 CR Caddisflies (Trichoptera) True Bugs (Hemiptera) Corixidae 1 14 I AF BVW MP 9 DV Dobsonflies (Megaloptera) Beetles (Coleoptera) Dytiscidae 1 14 I AF BVW PR 6 GN True Flies (Diptera) Chironomidae 61 847 I AF BVW CG 6 BU Simuliidae 3 42 I AF BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
ee
ff
Other Total Individuals 72 1001 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-4 STATION NAME Unnamed Tributary LAT (upstream) N 39deg 13035rsquo LONG (upstream) W 078deg 05129rsquo LAT (downstream) N 39deg 13000rsquo LONG (downstream) W 078deg 5086rsquo FORM COMPILED BY Andrew G M Fisher W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson A Fisher H Kepple
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Flatworms (Turbellaria) Worms (Oligochaeta) Lumbriculidae 2 32 A AF BVW CG 8 BU Leeches (Hirudinea) Snails (Gastropoda) Lymnaeidae 1 16 A BVW CG 9 GN Clams (Bivalvia) Isopods (Isopoda) Scuds (Amphipoda) Crayfish Shrimp (Decapoda) Cambaridae 1 16 A AF BVW GN 5 GN Water Mites (Hydracarina) Dragonflies (Odonata Anisoptera) Damselflies (Odonata Zygoptera) Calopterygidae 4 63 I BVW PR 7 CL Mayflies (Ephemeroptera) Tricorythidae 1 16 I BVW CG 5 SP Stoneflies (Plecoptera) Caddisflies (Trichoptera) Phryganeidae 4 63 I AF BVW SH 4 CR Hydropsychidae 6 95 I AF BVW CF 6 CG I True Bugs (Hemiptera) Corixidae 8 127 I AF BVW MP 9 DV (Veliidae ) (7) - I AF BVW ( ) Dobsonflies (Megaloptera) Sialidae 3 48 I AF BVW PR 7 SP Corydalidae 2 32 I AF BVW PR 5 CR Beetles (Coleoptera) Haliplidae 1 16 A AF BVW MP 8 GN Dysticidae 7 111 I AF BVW PR 6 GN Hydrophilidae 1 16 A AF BVW PR 8 GN Elmidae 3 48 I BVW SC 4 CG True Flies (Diptera) Chironomidae 8 127 I AF BVW CG 6 BU Simuliidae 7 111 I AF BVW CF 6 CG Tipulidae 4 63 I AF BVW SH 4 GU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
gg
hh
Other Total Individuals 63
(70) 1000
Total Taxa 17 (18)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Veliidae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-5 STATION NAME Lick Run LAT N 39deg 12752rsquo (upstream) LONG W 078deg 05685rsquo (upstream) LAT N 39deg 12784rsquo (downstream) LONG W 078deg 05631rsquo (downstream) FORM COMPILED BY Brandon Millholland W Bousquet RIVER BASIN Potomac River INVESTIGATORS J Pangle S Robertson W Bousquet JTovar M Dahl
Collection date 29 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Gomphidae 1 15 I BVW PR 3 BU Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Leptophlebiidae 11 167 A LBM BVW CG 2 CR Plecoptera (stoneflies) Perlidae 10 152 A LBM BVW PR 1 CR Perlodiae 1 15 A LBM BVW PR 2 CR Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 5 76 A LBM BVW SC 4 CG Elmidae 2 30 I LBM BVW SC 4 CG Diptera (true flies) (Culicidae) (3) - P LMB BVW ( ) Chironomidae 23 348 I LBM BVW CG 6 BU Simuliidae 13 197 I LBM BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
ii
jj
Other Total Individuals 66
(69) 1000
Total Taxa 7 (8)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Culicidae (Diptera) pupae not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl J Pangle S Robertson W Bousquet B Millholland J Tovar H Kepple
Collection date 30 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 1 12 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Asellidae 114 965 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Plecoptera (stoneflies) Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 2 23 A MRD BVW SC 4 CG Diptera (true flies)
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
kk
ll
Other Total Individuals 117 1000 Total Taxa 3
Notes Subsample taken 29 October 2009 All taxonomic determinations by MRD were confirmed by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson K McCann A Fisher H Kepple A Barrett
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 2 17 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Sphaeriidae 2 17 I MRD BVW CF 8 BU Isopoda (isopods) Asellidae 99 853 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Calopterygidae 2 17 A MRD BVW PR 7 CL Ephemeroptera (mayflies) Leptophlebiidae 2 17 I MRD BVW CG 2 CR Heptageniidae 4 34 A MRD BVW SC 3 CG Plecoptera (stoneflies) Perlidae 1 09 A MRD BVW PR 1 CR Trichoptera (caddisflies) Hemiptera (true bugs) (Gerridae) (1) - A MRD BVW ( ) Corixidae 1 09 A MRD BVW PR 5 CR Megaloptera (dobsonflies etc) Coleoptera (beetles) Dryopidae 1 09 A MRD BVW SC 5 CG Diptera (true flies) Tipulidae 2 17 I BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
mm
nn
Other Total Individuals 116
(117) 999
Total Taxa 10 (11)
Notes Subsample taken 6 November 2008 Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009 Gerridae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
- Disclaimer ii
- Title Page iii
- Acknowledgements v
- Table of Contents vii
- SECTION 2 RESULTS DATA TABLES 7
- OVERVIEW AND FINDINGS
- Introduction
- RESULTS DATA TABLES
- REFERENCES
- FIELD DATA SHEETS
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- SubstrateType
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- WATER QUALITY ANALYSIS
- THROUGH THE VIRGINIA
- STREAM CONDITION INDEX (VSCI)
- LABORATORY BENCH SHEETS
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
Site HLR-3 Route 664 Bridge
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 9 189 4762 EPT taxa 5 117 4273 Ephemeroptera 30 477 634 Plec +Tric less Hydropsych 61 562 1095 Scrapers 446 467 9556 Chironomidae 308 16 7037 Top 2 Dominant 723 328 4128 Hilsenhoff Biotic Index (HBI) 48 29 732VSCI Score 485
According to the DEQrsquos water quality standards aquatic life at this site is moderately stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 6 VSCI Scores for Route 664 Bridge (HLR-3) Site in Hiatt-Lick Run Sampled 28 May 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 7 189 3702 EPT taxa 4 117 3423 Ephemeroptera 40 477 844 Plec +Tric less Hydropsych 367 562 6535 Scrapers 122 467 2616 Chironomidae 00 16 10007 Top 2 Dominant 847 328 2288 Hilsenhoff Biotic Index (HBI) 42 29 817VSCI Score 469
According to the DEQrsquos water quality standards aquatic life at this site is moderately stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 7 VSCI Scores for Route 664 Bridge (HLR-3) Site in Hiatt-Lick Run Sampled 10 October 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
9
Site HLR-4 Unnamed Tributary
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 7 189 3702 EPT taxa 1 117 853 Ephemeroptera 00 477 004 Plec +Tric less Hydropsych 28 562 495 Scrapers 00 467 006 Chironomidae 847 16 1557 Top 2 Dominant 889 328 1658 Hilsenhoff Biotic Index (HBI) 60 29 563VSCI Score 173
According to the DEQrsquos water quality standards aquatic life at this site is severely stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 8 VSCI Scores for Unnamed Tributary (HLR-4) Site in Hiatt-Lick Run Sampled 31 May 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 17 189 8992 EPT taxa 3 117 2563 Ephemeroptera 16 477 344 Plec +Tric less Hydropsych 63 562 1125 Scrapers 48 467 1036 Chironomidae 127 16 8877 Top 2 Dominant 254 328 10008 Hilsenhoff Biotic Index (HBI) 63 29 521VSCI Score 476
According to the DEQrsquos water quality standards aquatic life at this site is moderately stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 9 VSCI Scores for Unnamed Tributary (HLR-4) Site in Hiatt-Lick Run Sampled 10 October 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
10
Site HLR-5 Lick Run
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 7 189 3702 EPT taxa 3 117 2563 Ephemeroptera 167 477 3504 Plec +Tric less Hydropsych 167 562 2975 Scrapers 106 467 2276 Chironomidae 348 16 6637 Top 2 Dominant 545 328 6778 Hilsenhoff Biotic Index (HBI) 43 29 803VSCI Score 455
According to the DEQrsquos water quality standards aquatic life at this site is moderately stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 10 VSCI Scores for Lick Run (HLR-5) Site in Hiatt-Lick Run Sampled 29 May 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
Note
Water conditions at the Lick Run (HLR-5) site were too low in October 2008 to permit a sample to be taken
11
Site HLR-6 McCann Farm
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 3 189 1592 EPT taxa 0 117 003 Ephemeroptera 00 477 004 Plec +Tric less Hydropsych 00 562 005 Scrapers 23 467 496 Chironomidae 00 16 10007 Top 2 Dominant 988 328 188 Hilsenhoff Biotic Index (HBI) 79 29 296VSCI Score 190
According to the DEQrsquos water quality standards aquatic life at this site is severely stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 11 VSCI Scores for McCann Farm (HLR-6) Site in Hiatt-Lick Run Sampled 30 May 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 10 189 5292 EPT taxa 3 117 2563 Ephemeroptera 51 477 1074 Plec +Tric less Hydropsych 09 562 165 Scrapers 43 467 926 Chironomidae 00 16 10007 Top 2 Dominant 887 328 1688 Hilsenhoff Biotic Index (HBI) 75 29 352VSCI Score 315
According to the DEQrsquos water quality standards aquatic life at this site is severely stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 12 VSCI Scores for McCann Farm (HLR-6) Site in Hiatt-Lick Run Sampled 10 October 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
12
REFERENCES
Barbour Michael T Jeroen Gerritsen Blaine D Snyder and James B Stribling 1999 Rapid bioassessment protocols for use in streams and wadeable rivers periphyton benthic macroinvertebrates and fish Second edition EPA 841-B-99-002 Washington DC US Environmental Protection Agency Office of Water Available online at httpwwwepagovOWOWmonitoringbioasspshtml Burton June and Jeroen Gerritsen 2003 A stream condition index for Virginia non-coastal streams Prepared for the US EPA Office of Science and Technology US EPA Region 3 Environmental Services Division and Virginia Department of Environmental Quality Owings Mills Maryland Tetra Tech Inc Available online at httpwwwdeqvirginiagovwatermonitoringpdf vastrmconpdf Hessler Rachel A Andrea R Martin Heidi D Ruggeri William B Seymour Jr and Woodward S Bousquet 2006 Water quality assessment using benthic macroinvertebrates A study and compilation Winchester Virginia Environmental Studies Program Shenandoah University Occasional publication no 10 Igoe Robert 2006 DEQ identifies nine impaired streams in Clarke Frederick The Winchester Star 15 July B1 Jackson Charlie 2005 Survey targets Opequon cleanup The Winchester Star 19 August B1 Johnston Elizabeth A and Woodward S Bousquet 2003 What happens when it rains A study of water quality in Abrams Creek and Town Run Winchester and Frederick County Virginia Winchester Virginia Environmental Studies Program Shenandoah University Occasional publication no7 Merritt Richard W and Kenneth W Cummins 1996 An introduction to the aquatic insects of North America Dubuque Iowa KendallHunt Publishing Company Opequon Creek IP Steering Committee Virginia Tech Department of Biological Systems Engineering and Center for TMDL and Watershed Studies University of Virginia Institute for Environmental Negotiation and West Virginia University Division of Resource Management 2006 Opequon Creek watershed TMDL implementation plan Blacksburg Virginia Virginia Tech Department of Biological Systems Engineering Available online at httpwwwtmdlbsevteduoutreachC82 Pennak Robert W 1953 Fresh-water invertebrates of the United States New York New York The Ronald Press Company Rosenburg David M and Vincent H Resh 1993 Freshwater biomonitoring and benthic macroinvertebrates New York New York Chapman amp Hall Shenandoah University Public Relations Office 2002 Data collected by SU and its partners helps identify impaired water Winchester Virginia Available online at httpwwwsuedutemp_ newscfmurlnum=32 Thorp James H and Alan P Covich 1991 Ecology and classification of North American freshwater invertebrates San Diego California Academic Press Inc
13
US Environmental Protection Agency 1997 Volunteer stream monitoring a methods manual EPA 841-B-97-003 Washington DC US Environmental Protection Agency Office of Water Van Meter Val 2003 Opequon Creek pollution limits to be established The Winchester Star 4 April B1 Virginia Department of Environmental Quality 2006a Virginia 305(b)303(d) water quality integrated report to Congress and the EPA Administrator for the period January 1 2000 to December 31 2004 Richmond Virginia Virginia Department of Environmental Quality Available online at httpwwwdeqvirginiagovwqair2006html __________ 2006b Using probabilistic data to validate the non-coastal Virginia Stream Condition Index (draft) VDEQ technical bulletin WQA2006-001 Virginia Department of Environmental Quality Available online at httpwwwloudounwatershedorgstream_assessmentreference_documents VA_DEQ_SCI_Validationpdf __________ 2008 Biological monitoring program quality assurance project plan for wadeable streams and rivers Richmond Virginia Virginia Department of Environmental Quality Update available online at httpwwwdeqstatevausbiomonitoringdocumentsBioMonQAPP_13Aug2008pdf Virginia Tech Aquatic Entomology Laboratory 2005 Taxa dictionary [Excel file] Blacksburg Virginia Virginia Tech Department of Biological Systems Engineering 2003a Opequon watershed TMDLs for benthic impairments Abrams Creek and Lower Opequon Creek Frederick and Clarke Counties Virginia Prepared for the Virginia Department of Environmental Quality and Virginia Department of Conservation and Recreation Blacksburg Virginia Available online at httpwwwdeqstate vaustmdlapptmdlsshenrvrabropebcpdf Voshell J Reese Jr 2002 A guide to common freshwater invertebrates of North America Blacksburg Virginia The McDonald amp Woodward Publishing Company
14
APPENDICES
15
16
a
Appendix A
FIELD DATA SHEETS
This appendix presents a Site Characterization Field Data Sheet for each of the six sampling reaches established on Hiatt Run and Lick Run for this study They were filled out during the sampling runs made in May 2008
These forms were adapted by Shenandoah University from forms used by the EPA and Virginia DEQ for recording characteristics of sampling sites
b
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Mouth STATION _HLR-1_____ STREAM CLASS LAT N 39 12749 (upstream) LONG W 078 04209(upstream) LAT N 39 12729 (downstream) LONG W 078 04148 (downstream) FORM COMPILED BY Jeremy Tovar RIVER BASIN Potomac River INVESTIGATORS JPangle B Millholland MDahl SRobertson W Bousquet JTovar BBousquet
DATE May 21 2008_________ TIME_250 PM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature __24___degC showers (intermittent) Comments _______________________
___ cloud cover _0_ ______________________________
X clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x ( Significant springs andor seeps) x (Valley ) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS
c
d
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence x FieldPasture Industrial Obvious sources Agricultural Other ____________ Stream scour steep slope erosion x Residential Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left Field and young thin deciduous forest 100 Bank=Grass cobble and shrub Right 90 maturing deciduous forest 45 shale slope Bank=Bedrock soil and herbs Indicate the dominant vegetation type(s) and record the dominant species
x Trees Shrubs x (Grasses) Forbs Dominant species Oak species and mix deciduous
INSTREAM FEATURES
Measured reach length ___100__m Canopy Cover Estimated stream width _35__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area __350_m2
Estimated stream depth _008_m Proportion of Reach Represented by Stream High water mark _15_m Morphology Types Surface velocity (at thalweg) _05__msec x Riffle__75___ x Run__20___ x Pool__5___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___00__ m2 Density of LWD _-_ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __15_____ x Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present Algae
WATER QUALITY Temperature __19___degC Water Odors x NormalNone Sewage Specific Conductance - Petroleum Chemical Fishy Other___________________ Dissolved Oxygen - Water Surface Oils Slick Sheen Globs Flecks pH __-_ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used _____NA________ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic x None Relict shells Other______None_____ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 8 Detritus sticks wood coarse plant materials
0 Boulder gt256 mm (10rsquorsquo) 0
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 51 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 40 Sand 006-2 mm (gritty) 1 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University
SITE CHARACTERIZATION FIELD DATA SHEET FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Package Plant STATION HLR-2 STREAM CLASS LAT N 39 129580 (upstream) LONG W 078 04900 (upstream) LAT N 39 129581 (downstream) LONG W 078 04822 (downstream) FORM COMPILED BY JW Pangle RIVER BASIN Potomac River INVESTIGATORS JPangle JTovar MDahl B Millholland SRobertson W Bousquet
DATE__19 May 2008_ TIME__1240__ PM
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature _____degC showers (intermittent) Comments __Sunny Day_____________
_5__ cloud cover _0__ ______________________________
x clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x (Significant springs andor seeps) x (Valley) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS
e
f
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence FieldPasture Industrial Obvious sources Agricultural Other ____________ __Upstream Construction and Stream bank Scour Residential __________________________________ Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left Deciduous forest with thick herb and shrub layers bank= vegetation and rocks Right Deciduous forest with thick herb and shrub layers bank=vegetation soil and rocks Indicate the dominant vegetation type(s) and record the dominant species
x Trees x Shrubs Grasses Forbs Dominant species Black Walnut White Ash Hackberry and American Sycamore
INSTREAM FEATURES
Measured reach length 100 m Canopy Cover Estimated stream width _35__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area __350_m2
Estimated stream depth __15__m Proportion of Reach Represented by Stream High water mark __22___m Morphology Types Surface velocity (at thalweg) _035__msec x Riffle__30___ x Run__30___ x Pool__40___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___10____ m2 Density of LWD _______ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __30___ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present__________________________________________________________
WATER QUALITY Temperature __18___degC Water Odors x NormalNone Sewage Specific Conductance __-____ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ____-____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used _____NA____ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other___None_____ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach SubstrateType Characteristic
Composition in Sampling Reach
Bedrock 3 Detritus sticks wood coarse plant materials
2 Boulder gt256 mm (10rsquorsquo) 5
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 70 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 22 Sand 006-2 mm (gritty) 0 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiattrsquos Run LOCATION Rt 664 Bridge STATION HLR-3 STREAM CLASS LAT N 39 12934 (upstream) LONG W 078 05019 (upstream) LAT N 39 12958 (downstream) LONG W 078 04958 (downstream) FORM COMPILED BY Sean Robertson RIVER BASIN Potomac River INVESTIGATORS SRobertson BMillholland WBousquet MDahl JPangle
DATE 28 May 2008 TIME 1127 AM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality 2008
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature __14___degC showers (intermittent) x Trace Comments _______________________
_65 x cloud cover ___ ______________________________
x clearsunny __________________________________
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x (Significant springs andor seeps) x (Valley) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS
g
h
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence Some evidence x FieldPasture Industrial x Obvious sources Agricultural x Other Old Package Plant Downstream x Residential Bridge and Road Power line and Pump station Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left 70 field 30 shrubs and deciduous trees Bank=Soil and vegetation Right Maturing deciduous forest steep slope Bank=Shale Bedrock Indicate the dominant vegetation type(s) and record the dominant species
x Trees x Shrubs x Grasses Forbs Dominant species Sycamore and Red Oaks
INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width __51___m Fully open x Partly open Partly shaded Fully shaded Sampling reach area _510_m2
Estimated stream depth __135___m Proportion of Reach Represented by Stream High water mark __-___m Morphology Types Surface velocity (at thalweg) __20___msec x Riffle_40_ x Run_50_ x Pool 10 Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___0____ m2 Density of LWD ___-____ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation ___50____ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present Brown Algae
WATER QUALITY Temperature __17___degC Water Odors x NormalNone Sewage Specific Conductance ___-___ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ___-_____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used ____NA_______ Clear x Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other____None____ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 15 Detritus sticks wood coarse plant materials
0 Boulder gt256 mm (10rsquorsquo) 15
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 62 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 8 Sand 006-2 mm (gritty) 0 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Unnamed Tributary STATION HLR-4 STREAM CLASS LAT N 39 13035 (upstream) LONG W 078 05129 (upstream) LAT N 39 13000 (downstream) LONG W 078 05086 (downstream) FORM COMPILED BY Marie Dahl RIVER BASIN Potomac River INVESTIGATORS M Dahl HKepple SRobertson AFisher
DATE 31 May 2008 TIME 5 PM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality 2008
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) YES x NO
rain (steady rain) Air Temperature __22___degC showers (intermittent) Comments _______________________
_0__ cloud cover _0__ ______________________________
x clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area __-___ km2 mi2 Montane Significant springs andor seeps x Valley Marsh or swamp Montane amp Valley Mixture of origins Other ___________________
COMMENTS Snowden Bridge development (upstream) and sewer line
i
j
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence x FieldPasture Industrial Obvious sources Agricultural Other ____________ Construction upstream and adjacent x Residential Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left Forested with rocky banks field across silt fence and 2m tree strip Right Forested with some rocky banks and forest through the highway Indicate the dominant vegetation type(s) and record the dominant species
x Trees x Shrubs x Grasses Forbs Dominant species White Ash and Hackberry
INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width ___38__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area __380__m2
Estimated stream depth __12__cm Proportion of Reach Represented by Stream High water mark __2___m Morphology Types Surface velocity (at thalweg) __46___msec x Riffle__25___ x Run__70___ x Pool__5___ Channelized Yes x No Dam Present x Yes No
LARGE WOODY DEBRIS
LWD _______ m2 Density of LWD _______ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation ___NA____ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present___Algae___
WATER QUALITY Temperature __16___degC Water Odors x NormalNone Sewage Specific Conductance __-____ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ___-_____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used ____NA_____ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells x Other___Shale_______ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 0 Detritus sticks wood coarse plant materials
10 Boulder gt256 mm (10rsquorsquo) 5
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 45 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 30 Sand 006-2 mm (gritty) 0 Marl grey shell
fragments 0
Silt 0004-006 mm 20 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Lick Run STATION HLR-5 STREAM CLASS LAT N 39 127517 (upstream) LONG W 078 05685(upstream) LAT N 39 12784 (downstream) LONG W 078 05631 (downstream) FORM COMPILED BY B Millholland RIVER BASIN Potomac River INVESTIGATORS B Millholland JTovar SRobertson MDahl WBousquet JPangle
DATE 29 May 2008 TIME 1040 AM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project
k
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature __15___degC showers (intermittent) Comments _______________________
_5_ X cloud cover _0__ ______________________________
x clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane Significant springs andor seeps x Valley Marsh or swamp Montane amp Valley Mixture of origins Other ___________________
COMMENTS Lick Run appears to be intermittent and may be dry in Fall 2008
l
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence x FieldPasture Industrial Obvious sources Agricultural Other ____________ Adjacent logging stream bank scour Residential Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left 85 young deciduous forest 15 logged Bank=Soil vegetation and cobbles Right Young deciduous forest on 30 slope Bank= Soil vegetation and cobble Indicate the dominant vegetation type(s) and record the dominant species
x Trees Shrubs Grasses Forbs Dominant species Mixed Deciduous INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width __11__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area 110 m2
Estimated stream depth _009__m Proportion of Reach Represented by Stream High water mark __96___m Morphology Types Surface velocity (at thalweg) __01___msec x Riffle__30___ x Run__5___ x Pool__65___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___0____ m2 Density of LWD ___-____ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __70___ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present__________________________________________________________
WATER QUALITY Temperature __14___degC Water Odors x NormalNone Sewage Specific Conductance __-__ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ___-___ Water Surface Oils Slick Sheen Globs Flecks pH __-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used _____NA______ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other_____None_____
SEDIMENT AND SUBSTRATE
Odors Deposits Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other________________ x Other Slightly Salty Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 0 Detritus sticks wood coarse plant materials
5 Boulder gt256 mm (10rsquorsquo) 0
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 75 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 23 Sand 006-2 mm (gritty) 2 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION McCann Farm STATION HLR-6 STREAM CLASS LAT N 39 13076 (upstream) LONG W 078 06539 (upstream) LAT N 39 13063 (downstream) LONG W 078 06473 (downstream) FORM COMPILED BY MDahl RIVER BASIN Potomac River INVESTIGATORS MDahl JPangle SRobertson W Bousqet BMillholland JTovar H Kepple
DATE 30 May 2008 TIME 1050 AM (start)
PROJECT NAME
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) YES x NO
rain (steady rain) Air Temperature __18___degC showers (intermittent) Comments _______________________
_85_x cloud cover _10__ ______________________________
clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x (Significant springs andor seeps) x ( Valley) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS Multi-habitat sampling with D-frame nets and seine
m
n
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial x No evidence Some evidence x FieldPasture Industrial Obvious sources x Agricultural Other ____________ Minor stream bank scour Residential Local Watershed Erosion x None Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left 35 bank grasses scattered trees and some bushes Right High bank with grasses and scattered trees Indicate the dominant vegetation type(s) and record the dominant species
x Trees Shrubs Grasses Forbs Dominant species Black Walnut and Festuca elatior
INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width __2___m Fully open Partly open x Partly shaded Fully shaded Sampling reach area __200_m2
Estimated stream depth __03_m Proportion of Reach Represented by Stream High water mark __1___m Morphology Types Surface velocity (at thalweg) __166_msec x Riffle__0___ x Run__55___ x Pool__45___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___2____ m2 Density of LWD ___-____ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __20_____ x Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present ____Glyceria (grass)sp__________
WATER QUALITY Temperature __16___degC Water Odors x NormalNone Sewage Specific Conductance ___-___ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ____-____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used ______NA___ Clear x Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other__NONE__ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 0 Detritus sticks wood coarse plant materials
5 Boulder gt256 mm (10rsquorsquo) 0
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 27 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 38 Sand 006-2 mm (gritty) 5 Marl grey shell
fragments 0 (lt1)
Silt 0004-006 mm 25 Clay lt 0004 mm (slick) 5 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Appendix B
WATER QUALITY ANALYSIS THROUGH THE VIRGINIA
STREAM CONDITION INDEX (VSCI)
The Virginia Stream Condition Index (VSCI) relies on eight metrics to categorize stream health According to Burton and Gerritsen (2003 p 1-1) this index ldquohellip can be used as a primary indicator of ecosystem health and can identify impairment with respect to the reference (or natural) conditionrdquo These metrics are numeric measures of a biological communityrsquos species composition diversity ecological niches or tolerance to pollution
Table 13 on the next page describes each VSCI metric lists its formula and provides the best standard value (BSV) for that metric Based on a 100-point scale the BSV is the score that reflects optimum conditions for aquatic life ie the best water quality value possible (DEQ 2006b pp 8 33) The table also explains whether the score increases or decreases with environmental stress
As Table 13 indicates each of the eight VSCI metrics is calculated individually Each formula provides a unitless score based on a 100-point scale with 0 as the worst possible score and 100 as the best Any metric that provides a result above 100 is changed to 100 in order for the index to work properly After the eight metrics are calculated they are summed and averaged to provide a final VSCI score on a 100-point scale
Based on statistical analysis of Virginiarsquos streams and their BMI communities the DEQ (2006b p 8) currently uses the following tiers to interpret VSCI scores
VSCI Scores Aquatic Life Use
(ALI) Tiers
lt 42 severely stressed
42-55 moderately stressed
55-63 unknownfair (gray zone)
63-73 good
gt73 excellent
Section 2 of this report presents the results of calculations for the six BMI sampling sites the research team studied in the Hiatt-Lick Run subwatershed in 2008
o
p
1 Total taxa the number of distinct taxa in the sample measures the overall diversity of the macroinvertebrate assemblage The best standard value (BSV) is 189 Score = 100 x (X189) Total taxa decreases with stress 2 EPT taxa the sum of the distinct taxa in the orders Ephemeroptera (mayflies) Plecoptera (stoneflies) and Trichoptera (caddisflies) These orders are known to be sensitive to pollution The best standard value (BSV) is 117 Score = 100 x (X117) EPT taxa decreases with stress 3 Percent Ephemeroptera the percent abundance of individuals in the sample that belong to the order Ephemeroptera (mayflies) The best standard value (BSV) is 477 Score = 100 x (X477) Percent Ephemeroptera decreases with stress 4 Percent Plecoptera + Trichoptera less Hydropsychidae the percent abundance of individuals in the sample that belong to the orders Plecoptera (stoneflies) and Trichoptera (caddisflies) excluding the pollution-tolerant caddisfly family Hydropsychidae The best standard value (BSV) is 562 Score = 100 x (X562) Percent Plecoptera + Trichoptera less Hydropsychidae decreases with stress 5 Percent Scrapers the percent abundance of individuals in the sample whose primary functional mechanism for feeding is grazing on the substrate or periphyton (ie attached algae and associated material) The best standard value (BSV) is 467 Score = 100 x (X467) Percent scrapers decreases with stress 6 Percent Chironomidae the percent abundance of individuals in the sample that are Chironomidae (non-biting midge) larvae and pupae The best standard value (BSV) is 16 Score = 100 x [(100-X)(100-16)] Percent Chironomidae increases with stress 7 Percent Top Two Dominant the percent abundance of the two most frequent taxa present in the sample The best standard value (BSV) is 328 Score = 100 x [(100-X)(100-328)] The Percent Top 2 Dominant increases with stress 8 Hilsenhoff Biotic Index (HBI) an abundance-weighted average pollution tolerance of all the individuals within a sample keyed to the family level Tolerance ratings range from 1 (highly intolerant) to 10 (highly tolerant) The best standard value (BSV) is 29 Score = 100 x [(10-X)(10-29)] The Hilsenhoff Biotic Index (HBI) increases with stress ______________________________________________________________________________ Table 13 Description of Benthic Macroinvertebrate VSCI Metrics Includes responses (increases decreases) of each VSCI metric to degraded water quality conditions Adapted from Burton and Gerritsen (2003 pp 3-15 3-16 6-2) and DEQ (2008)
q
Appendix C
LABORATORY BENCH SHEETS
Shenandoah Universityrsquos Environmental Studies Department developed the Benthic Macroinvertebrate Laboratory Bench Sheet and VSCI Worksheet to record BMI specimen identifications and their VSCI classifications for each sample
This form is based in part on bench sheets developed by the EPA and Virginia DEQ for recording specimen identifications Additional columns on Shenandoah Universityrsquos forms for relative numbers and VSCI classifications make the calculation and verification of VSCI scores easier
Note the column for taxonomistrsquos initials Specimens were identified by students in Shenandoah Universityrsquos research group and then verified or where necessary changed by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality This external review helps assure the validity of the study Originals of these bench sheets with handwritten taxonomistsrsquo initials will be kept on file at Shenandoah Universityrsquos Environmental Studies Department for five years after the completion of this study
r
s
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-1 STATION NAME Mouth LAT N 39deg 12749rsquo (upstream) LONG W 078deg 04209rsquo (upstream) LAT N 39deg 12729rsquo (downstream) LONG W 078deg 04148rsquo (downstream) FORM COMPILED BY Jeremy Tovar W S Bousquet RIVER BASIN Potomac River INVESTIGATORS Marie Dahl Brandon Millholand J W Pangle Sean Robertson Jeremy Tovar Woodward Bousquet Ben Bousquet
Collection date 21 May 2008 Sheet completion date 12 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Hirudinea 3 33 JT BVW PR 8 SP Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Coenagrionidae 2 22 BVW PR 9 CR Ephemeroptera (mayflies) Caenidae 1 11 JT BVW CG 6 SP Ephemerllidae 4 44 JT BVW CG 2 CR Heptageniidae 5 55 JT BVW SC 3 CG Isonychiidae 1 11 BVW CF 3 CG Plecoptera (stoneflies) Perlidae 14 154 JT BVW PR 2 CR Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Corydalidae 1 11 JT BVW PR 5 CR Coleoptera (beetles) Elmidae 24 264 I JT BVW SC 4 CG Dytiscidae 4 44 JT BVW PR 6 GN Psephenidae 3 33 JT BVW SC 4 CG Diptera (true flies) Tipulidae 3 33 JT BVW SH 4 BU Chironomidae 26 286 JT BVW CG 6 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
t
Other Total Individuals 91 1001 Total Taxa 13
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
u
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-1 STATION NAME Mouth LAT N 39deg 12749rsquo (upstream) LONG W 078deg 04209rsquo (upstream) LAT N 39deg 12729rsquo (downstream) LONG W 078deg 04148rsquo (downstream) FORM COMPILED BY Jeremy Tovar W S Bousquet RIVER BASIN Potomac River INVESTIGATORS Brandon Millholand JW Pangle Jeremy Tovar Heather Kepple
Collection date 10 October 2008 Sheet completion date 12 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Oligochaeta 3 35 JT BVW CG 8 BU Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Coenagrionidae 2 23 JT BVW PR 9 CR Ephemeroptera (mayflies) Heptageniidae 12 140 JT BVW SC 3 CG Isonychiidae 2 23 BVW CF 3 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Hydropsychidae 16 186 JT BVW CF 6 CG Philopotamidae 31 360 JT BVW CF 2 CG Hemiptera (true bugs) (Veliidae) (5) - JT BVW ( ) Megaloptera (dobsonflies etc) Corydalidae 10 116 JT BVW PR 5 CR Coleoptera (beetles) Dytiscidae 1 12 JT BVW PR 6 GN Psephenidae 7 81 JT BVW SC 4 CG Diptera (true flies) Tipulidae 2 23 BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
v
Other Total Individuals 86
(91) 999
Total Taxa 10 (11)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009 Veliidae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
w
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-2 STATION NAME Package Plant LAT N 39deg 12958rsquo (upstream) LONG W 078deg 04900 (upstream) LAT N 39deg 12958rsquo (downstream) LONG W 078deg 04822 (downstream) FORM COMPILED BY JW Pangle W Bousquet RIVER BASIN Potomac River INVESTIGATORS JW Pangle S Robertson J Tovar M Dahl B Millholland W Bousquet
Collection date 29 May 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Plecoptera (stoneflies) Perlidae 11 136 I JP BVW PR 1 CR Trichoptera (caddisflies) Hydropsychidae 10 123 I JP BVW CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 14 173 I JP BVW SC 4 CG Diptera (true flies) Chironomidae 32 395 I JP BVW CG 6 BU Simuliidae 14 173 I JP BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
x
Other Total Individuals 81 1000 Total Taxa 5
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-2 STATION NAME Package Plant LAT N 39deg 12958rsquo (upstream) LONG W 078deg 04900 (upstream) LAT N 39deg 12958rsquo (downstream) LONG W 078deg 04822 (downstream) FORM COMPILED BY JW Pangle W Bousquet RIVER BASIN Potomac River INVESTIGATORS JW Pangle Brandon Millholland Jeremy Tovar Heather Kepple
Collection date 10 October 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Heptageniidae 40 392 I WBJP SC 3 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Hydropsychidae 7 69 I JP WB BVW CF 6 CG Philopotamidae 28 275 I JP WB BVW CF 2 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Corydalidae 6 59 I JP WB BVW PR 5 CR Coleoptera (beetles) Psephenidae 7 69 I JP WB BVW SC 4 CG Elmidae 8 78 I JP WB BVW SC 4 CG Diptera (true flies) Tipulidae 6 59 I JP WB BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
y
z
Other Total Individuals 102 1001 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-3 STATION NAME Rt 664 Bridge LAT N 39deg 12934rsquo (upstream) LONG W 078deg 05019rsquo (upstream) LAT N 39deg 12951rsquo (downstream) LONG W 078deg 04958rsquo (downstream) FORM COMPILED BY S Robertson W Bousquet RIVER BASIN Potomac River INVESTIGATORS SRobertson BMillholland WBousquet MDahl JPangle
Collection date 28 May 2008 Sheet completion date 11 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Leptophlebiidae 1 15 I SGR BVW CG 2 CR Baetidae 1 15 I BVW CG 5 CG Plecoptera (stoneflies) Perlidae 3 46 I SGR BVW PR 1 CR Nemouridae 1 15 I BVW SH 3 CR Trichoptera (caddisflies) Hydropsychidae 5 77 I SGR BVW CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 27 415 I SGR BVW SC 4 CG Psephenidae 2 31 A SGR BVW SC 4 CG Diptera (true flies) Simuliidae 5 77 I SGR BVW CF 6 CG Chironomidae 20 308 I SGR BVW CG 6 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
aa
bb
Other Total Individuals 65 999 Total Taxa 9
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-3 STATION NAME Rt 664 Bridge LAT N 39deg 12934rsquo (upstream) LONG W 078deg 05019rsquo (upstream) LAT N 39deg 12951rsquo (downstream) LONG W 078deg 04958rsquo (downstream) FORM COMPILED BY S Robertson RIVER BASIN Potomac River INVESTIGATORS S Robertson M Dahl A Fisher H Kepple
Collection date 10 Oct 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Heptageniidae 2 20 I SGR SC 3 CG Isonychiidae 2 20 I BVW CF 2 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Philopotamidae 36 367 I SGR CF 2 CG Hydropsychidae 47 480 I SGR CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 2 20 A SGR SC 4 CG Psephenidae 8 82 A SGR SC 4 CG Diptera (true flies) Tabanidae 1 10 I SGR PR 7 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
cc
dd
Other Total Individuals 98 999 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-4 STATION NAME Unnamed Tributary LAT (upstream) N 39deg 13035rsquo LONG (upstream) W 078deg 05129rsquo LAT (downstream) N 39deg 13000rsquo LONG (downstream) W 078deg 5086rsquo FORM COMPILED BY A Fisher M Dahl rev W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson A Fisher H Kepple
Collection date 31 May 2008 Sheet completion date rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Flatworms (Turbellaria) Worms (Oligochaeta) Naididae 1 14 - BVW CG 8 BU Leeches (Hirudinea) Snails (Gastropoda) Clams (Bivalvia) Isopods (Isopoda) Asellidae 3 42 A AF BVW CG 8 SP Scuds (Amphipoda) Crayfish Shrimp (Decapoda) Water Mites (Hydracarina) Dragonflies (Odonata Anisoptera) Damselflies (Odonata Zygoptera) Mayflies (Ephemeroptera) Stoneflies (Plecoptera) Perlidae 2 28 I AF BVW PR 1 CR Caddisflies (Trichoptera) True Bugs (Hemiptera) Corixidae 1 14 I AF BVW MP 9 DV Dobsonflies (Megaloptera) Beetles (Coleoptera) Dytiscidae 1 14 I AF BVW PR 6 GN True Flies (Diptera) Chironomidae 61 847 I AF BVW CG 6 BU Simuliidae 3 42 I AF BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
ee
ff
Other Total Individuals 72 1001 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-4 STATION NAME Unnamed Tributary LAT (upstream) N 39deg 13035rsquo LONG (upstream) W 078deg 05129rsquo LAT (downstream) N 39deg 13000rsquo LONG (downstream) W 078deg 5086rsquo FORM COMPILED BY Andrew G M Fisher W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson A Fisher H Kepple
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Flatworms (Turbellaria) Worms (Oligochaeta) Lumbriculidae 2 32 A AF BVW CG 8 BU Leeches (Hirudinea) Snails (Gastropoda) Lymnaeidae 1 16 A BVW CG 9 GN Clams (Bivalvia) Isopods (Isopoda) Scuds (Amphipoda) Crayfish Shrimp (Decapoda) Cambaridae 1 16 A AF BVW GN 5 GN Water Mites (Hydracarina) Dragonflies (Odonata Anisoptera) Damselflies (Odonata Zygoptera) Calopterygidae 4 63 I BVW PR 7 CL Mayflies (Ephemeroptera) Tricorythidae 1 16 I BVW CG 5 SP Stoneflies (Plecoptera) Caddisflies (Trichoptera) Phryganeidae 4 63 I AF BVW SH 4 CR Hydropsychidae 6 95 I AF BVW CF 6 CG I True Bugs (Hemiptera) Corixidae 8 127 I AF BVW MP 9 DV (Veliidae ) (7) - I AF BVW ( ) Dobsonflies (Megaloptera) Sialidae 3 48 I AF BVW PR 7 SP Corydalidae 2 32 I AF BVW PR 5 CR Beetles (Coleoptera) Haliplidae 1 16 A AF BVW MP 8 GN Dysticidae 7 111 I AF BVW PR 6 GN Hydrophilidae 1 16 A AF BVW PR 8 GN Elmidae 3 48 I BVW SC 4 CG True Flies (Diptera) Chironomidae 8 127 I AF BVW CG 6 BU Simuliidae 7 111 I AF BVW CF 6 CG Tipulidae 4 63 I AF BVW SH 4 GU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
gg
hh
Other Total Individuals 63
(70) 1000
Total Taxa 17 (18)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Veliidae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-5 STATION NAME Lick Run LAT N 39deg 12752rsquo (upstream) LONG W 078deg 05685rsquo (upstream) LAT N 39deg 12784rsquo (downstream) LONG W 078deg 05631rsquo (downstream) FORM COMPILED BY Brandon Millholland W Bousquet RIVER BASIN Potomac River INVESTIGATORS J Pangle S Robertson W Bousquet JTovar M Dahl
Collection date 29 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Gomphidae 1 15 I BVW PR 3 BU Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Leptophlebiidae 11 167 A LBM BVW CG 2 CR Plecoptera (stoneflies) Perlidae 10 152 A LBM BVW PR 1 CR Perlodiae 1 15 A LBM BVW PR 2 CR Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 5 76 A LBM BVW SC 4 CG Elmidae 2 30 I LBM BVW SC 4 CG Diptera (true flies) (Culicidae) (3) - P LMB BVW ( ) Chironomidae 23 348 I LBM BVW CG 6 BU Simuliidae 13 197 I LBM BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
ii
jj
Other Total Individuals 66
(69) 1000
Total Taxa 7 (8)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Culicidae (Diptera) pupae not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl J Pangle S Robertson W Bousquet B Millholland J Tovar H Kepple
Collection date 30 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 1 12 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Asellidae 114 965 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Plecoptera (stoneflies) Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 2 23 A MRD BVW SC 4 CG Diptera (true flies)
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
kk
ll
Other Total Individuals 117 1000 Total Taxa 3
Notes Subsample taken 29 October 2009 All taxonomic determinations by MRD were confirmed by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson K McCann A Fisher H Kepple A Barrett
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 2 17 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Sphaeriidae 2 17 I MRD BVW CF 8 BU Isopoda (isopods) Asellidae 99 853 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Calopterygidae 2 17 A MRD BVW PR 7 CL Ephemeroptera (mayflies) Leptophlebiidae 2 17 I MRD BVW CG 2 CR Heptageniidae 4 34 A MRD BVW SC 3 CG Plecoptera (stoneflies) Perlidae 1 09 A MRD BVW PR 1 CR Trichoptera (caddisflies) Hemiptera (true bugs) (Gerridae) (1) - A MRD BVW ( ) Corixidae 1 09 A MRD BVW PR 5 CR Megaloptera (dobsonflies etc) Coleoptera (beetles) Dryopidae 1 09 A MRD BVW SC 5 CG Diptera (true flies) Tipulidae 2 17 I BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
mm
nn
Other Total Individuals 116
(117) 999
Total Taxa 10 (11)
Notes Subsample taken 6 November 2008 Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009 Gerridae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
- Disclaimer ii
- Title Page iii
- Acknowledgements v
- Table of Contents vii
- SECTION 2 RESULTS DATA TABLES 7
- OVERVIEW AND FINDINGS
- Introduction
- RESULTS DATA TABLES
- REFERENCES
- FIELD DATA SHEETS
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- SubstrateType
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- WATER QUALITY ANALYSIS
- THROUGH THE VIRGINIA
- STREAM CONDITION INDEX (VSCI)
- LABORATORY BENCH SHEETS
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
Site HLR-4 Unnamed Tributary
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 7 189 3702 EPT taxa 1 117 853 Ephemeroptera 00 477 004 Plec +Tric less Hydropsych 28 562 495 Scrapers 00 467 006 Chironomidae 847 16 1557 Top 2 Dominant 889 328 1658 Hilsenhoff Biotic Index (HBI) 60 29 563VSCI Score 173
According to the DEQrsquos water quality standards aquatic life at this site is severely stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 8 VSCI Scores for Unnamed Tributary (HLR-4) Site in Hiatt-Lick Run Sampled 31 May 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 17 189 8992 EPT taxa 3 117 2563 Ephemeroptera 16 477 344 Plec +Tric less Hydropsych 63 562 1125 Scrapers 48 467 1036 Chironomidae 127 16 8877 Top 2 Dominant 254 328 10008 Hilsenhoff Biotic Index (HBI) 63 29 521VSCI Score 476
According to the DEQrsquos water quality standards aquatic life at this site is moderately stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 9 VSCI Scores for Unnamed Tributary (HLR-4) Site in Hiatt-Lick Run Sampled 10 October 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
10
Site HLR-5 Lick Run
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 7 189 3702 EPT taxa 3 117 2563 Ephemeroptera 167 477 3504 Plec +Tric less Hydropsych 167 562 2975 Scrapers 106 467 2276 Chironomidae 348 16 6637 Top 2 Dominant 545 328 6778 Hilsenhoff Biotic Index (HBI) 43 29 803VSCI Score 455
According to the DEQrsquos water quality standards aquatic life at this site is moderately stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 10 VSCI Scores for Lick Run (HLR-5) Site in Hiatt-Lick Run Sampled 29 May 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
Note
Water conditions at the Lick Run (HLR-5) site were too low in October 2008 to permit a sample to be taken
11
Site HLR-6 McCann Farm
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 3 189 1592 EPT taxa 0 117 003 Ephemeroptera 00 477 004 Plec +Tric less Hydropsych 00 562 005 Scrapers 23 467 496 Chironomidae 00 16 10007 Top 2 Dominant 988 328 188 Hilsenhoff Biotic Index (HBI) 79 29 296VSCI Score 190
According to the DEQrsquos water quality standards aquatic life at this site is severely stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 11 VSCI Scores for McCann Farm (HLR-6) Site in Hiatt-Lick Run Sampled 30 May 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 10 189 5292 EPT taxa 3 117 2563 Ephemeroptera 51 477 1074 Plec +Tric less Hydropsych 09 562 165 Scrapers 43 467 926 Chironomidae 00 16 10007 Top 2 Dominant 887 328 1688 Hilsenhoff Biotic Index (HBI) 75 29 352VSCI Score 315
According to the DEQrsquos water quality standards aquatic life at this site is severely stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 12 VSCI Scores for McCann Farm (HLR-6) Site in Hiatt-Lick Run Sampled 10 October 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
12
REFERENCES
Barbour Michael T Jeroen Gerritsen Blaine D Snyder and James B Stribling 1999 Rapid bioassessment protocols for use in streams and wadeable rivers periphyton benthic macroinvertebrates and fish Second edition EPA 841-B-99-002 Washington DC US Environmental Protection Agency Office of Water Available online at httpwwwepagovOWOWmonitoringbioasspshtml Burton June and Jeroen Gerritsen 2003 A stream condition index for Virginia non-coastal streams Prepared for the US EPA Office of Science and Technology US EPA Region 3 Environmental Services Division and Virginia Department of Environmental Quality Owings Mills Maryland Tetra Tech Inc Available online at httpwwwdeqvirginiagovwatermonitoringpdf vastrmconpdf Hessler Rachel A Andrea R Martin Heidi D Ruggeri William B Seymour Jr and Woodward S Bousquet 2006 Water quality assessment using benthic macroinvertebrates A study and compilation Winchester Virginia Environmental Studies Program Shenandoah University Occasional publication no 10 Igoe Robert 2006 DEQ identifies nine impaired streams in Clarke Frederick The Winchester Star 15 July B1 Jackson Charlie 2005 Survey targets Opequon cleanup The Winchester Star 19 August B1 Johnston Elizabeth A and Woodward S Bousquet 2003 What happens when it rains A study of water quality in Abrams Creek and Town Run Winchester and Frederick County Virginia Winchester Virginia Environmental Studies Program Shenandoah University Occasional publication no7 Merritt Richard W and Kenneth W Cummins 1996 An introduction to the aquatic insects of North America Dubuque Iowa KendallHunt Publishing Company Opequon Creek IP Steering Committee Virginia Tech Department of Biological Systems Engineering and Center for TMDL and Watershed Studies University of Virginia Institute for Environmental Negotiation and West Virginia University Division of Resource Management 2006 Opequon Creek watershed TMDL implementation plan Blacksburg Virginia Virginia Tech Department of Biological Systems Engineering Available online at httpwwwtmdlbsevteduoutreachC82 Pennak Robert W 1953 Fresh-water invertebrates of the United States New York New York The Ronald Press Company Rosenburg David M and Vincent H Resh 1993 Freshwater biomonitoring and benthic macroinvertebrates New York New York Chapman amp Hall Shenandoah University Public Relations Office 2002 Data collected by SU and its partners helps identify impaired water Winchester Virginia Available online at httpwwwsuedutemp_ newscfmurlnum=32 Thorp James H and Alan P Covich 1991 Ecology and classification of North American freshwater invertebrates San Diego California Academic Press Inc
13
US Environmental Protection Agency 1997 Volunteer stream monitoring a methods manual EPA 841-B-97-003 Washington DC US Environmental Protection Agency Office of Water Van Meter Val 2003 Opequon Creek pollution limits to be established The Winchester Star 4 April B1 Virginia Department of Environmental Quality 2006a Virginia 305(b)303(d) water quality integrated report to Congress and the EPA Administrator for the period January 1 2000 to December 31 2004 Richmond Virginia Virginia Department of Environmental Quality Available online at httpwwwdeqvirginiagovwqair2006html __________ 2006b Using probabilistic data to validate the non-coastal Virginia Stream Condition Index (draft) VDEQ technical bulletin WQA2006-001 Virginia Department of Environmental Quality Available online at httpwwwloudounwatershedorgstream_assessmentreference_documents VA_DEQ_SCI_Validationpdf __________ 2008 Biological monitoring program quality assurance project plan for wadeable streams and rivers Richmond Virginia Virginia Department of Environmental Quality Update available online at httpwwwdeqstatevausbiomonitoringdocumentsBioMonQAPP_13Aug2008pdf Virginia Tech Aquatic Entomology Laboratory 2005 Taxa dictionary [Excel file] Blacksburg Virginia Virginia Tech Department of Biological Systems Engineering 2003a Opequon watershed TMDLs for benthic impairments Abrams Creek and Lower Opequon Creek Frederick and Clarke Counties Virginia Prepared for the Virginia Department of Environmental Quality and Virginia Department of Conservation and Recreation Blacksburg Virginia Available online at httpwwwdeqstate vaustmdlapptmdlsshenrvrabropebcpdf Voshell J Reese Jr 2002 A guide to common freshwater invertebrates of North America Blacksburg Virginia The McDonald amp Woodward Publishing Company
14
APPENDICES
15
16
a
Appendix A
FIELD DATA SHEETS
This appendix presents a Site Characterization Field Data Sheet for each of the six sampling reaches established on Hiatt Run and Lick Run for this study They were filled out during the sampling runs made in May 2008
These forms were adapted by Shenandoah University from forms used by the EPA and Virginia DEQ for recording characteristics of sampling sites
b
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Mouth STATION _HLR-1_____ STREAM CLASS LAT N 39 12749 (upstream) LONG W 078 04209(upstream) LAT N 39 12729 (downstream) LONG W 078 04148 (downstream) FORM COMPILED BY Jeremy Tovar RIVER BASIN Potomac River INVESTIGATORS JPangle B Millholland MDahl SRobertson W Bousquet JTovar BBousquet
DATE May 21 2008_________ TIME_250 PM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature __24___degC showers (intermittent) Comments _______________________
___ cloud cover _0_ ______________________________
X clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x ( Significant springs andor seeps) x (Valley ) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS
c
d
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence x FieldPasture Industrial Obvious sources Agricultural Other ____________ Stream scour steep slope erosion x Residential Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left Field and young thin deciduous forest 100 Bank=Grass cobble and shrub Right 90 maturing deciduous forest 45 shale slope Bank=Bedrock soil and herbs Indicate the dominant vegetation type(s) and record the dominant species
x Trees Shrubs x (Grasses) Forbs Dominant species Oak species and mix deciduous
INSTREAM FEATURES
Measured reach length ___100__m Canopy Cover Estimated stream width _35__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area __350_m2
Estimated stream depth _008_m Proportion of Reach Represented by Stream High water mark _15_m Morphology Types Surface velocity (at thalweg) _05__msec x Riffle__75___ x Run__20___ x Pool__5___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___00__ m2 Density of LWD _-_ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __15_____ x Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present Algae
WATER QUALITY Temperature __19___degC Water Odors x NormalNone Sewage Specific Conductance - Petroleum Chemical Fishy Other___________________ Dissolved Oxygen - Water Surface Oils Slick Sheen Globs Flecks pH __-_ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used _____NA________ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic x None Relict shells Other______None_____ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 8 Detritus sticks wood coarse plant materials
0 Boulder gt256 mm (10rsquorsquo) 0
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 51 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 40 Sand 006-2 mm (gritty) 1 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University
SITE CHARACTERIZATION FIELD DATA SHEET FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Package Plant STATION HLR-2 STREAM CLASS LAT N 39 129580 (upstream) LONG W 078 04900 (upstream) LAT N 39 129581 (downstream) LONG W 078 04822 (downstream) FORM COMPILED BY JW Pangle RIVER BASIN Potomac River INVESTIGATORS JPangle JTovar MDahl B Millholland SRobertson W Bousquet
DATE__19 May 2008_ TIME__1240__ PM
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature _____degC showers (intermittent) Comments __Sunny Day_____________
_5__ cloud cover _0__ ______________________________
x clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x (Significant springs andor seeps) x (Valley) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS
e
f
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence FieldPasture Industrial Obvious sources Agricultural Other ____________ __Upstream Construction and Stream bank Scour Residential __________________________________ Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left Deciduous forest with thick herb and shrub layers bank= vegetation and rocks Right Deciduous forest with thick herb and shrub layers bank=vegetation soil and rocks Indicate the dominant vegetation type(s) and record the dominant species
x Trees x Shrubs Grasses Forbs Dominant species Black Walnut White Ash Hackberry and American Sycamore
INSTREAM FEATURES
Measured reach length 100 m Canopy Cover Estimated stream width _35__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area __350_m2
Estimated stream depth __15__m Proportion of Reach Represented by Stream High water mark __22___m Morphology Types Surface velocity (at thalweg) _035__msec x Riffle__30___ x Run__30___ x Pool__40___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___10____ m2 Density of LWD _______ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __30___ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present__________________________________________________________
WATER QUALITY Temperature __18___degC Water Odors x NormalNone Sewage Specific Conductance __-____ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ____-____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used _____NA____ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other___None_____ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach SubstrateType Characteristic
Composition in Sampling Reach
Bedrock 3 Detritus sticks wood coarse plant materials
2 Boulder gt256 mm (10rsquorsquo) 5
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 70 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 22 Sand 006-2 mm (gritty) 0 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiattrsquos Run LOCATION Rt 664 Bridge STATION HLR-3 STREAM CLASS LAT N 39 12934 (upstream) LONG W 078 05019 (upstream) LAT N 39 12958 (downstream) LONG W 078 04958 (downstream) FORM COMPILED BY Sean Robertson RIVER BASIN Potomac River INVESTIGATORS SRobertson BMillholland WBousquet MDahl JPangle
DATE 28 May 2008 TIME 1127 AM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality 2008
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature __14___degC showers (intermittent) x Trace Comments _______________________
_65 x cloud cover ___ ______________________________
x clearsunny __________________________________
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x (Significant springs andor seeps) x (Valley) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS
g
h
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence Some evidence x FieldPasture Industrial x Obvious sources Agricultural x Other Old Package Plant Downstream x Residential Bridge and Road Power line and Pump station Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left 70 field 30 shrubs and deciduous trees Bank=Soil and vegetation Right Maturing deciduous forest steep slope Bank=Shale Bedrock Indicate the dominant vegetation type(s) and record the dominant species
x Trees x Shrubs x Grasses Forbs Dominant species Sycamore and Red Oaks
INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width __51___m Fully open x Partly open Partly shaded Fully shaded Sampling reach area _510_m2
Estimated stream depth __135___m Proportion of Reach Represented by Stream High water mark __-___m Morphology Types Surface velocity (at thalweg) __20___msec x Riffle_40_ x Run_50_ x Pool 10 Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___0____ m2 Density of LWD ___-____ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation ___50____ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present Brown Algae
WATER QUALITY Temperature __17___degC Water Odors x NormalNone Sewage Specific Conductance ___-___ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ___-_____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used ____NA_______ Clear x Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other____None____ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 15 Detritus sticks wood coarse plant materials
0 Boulder gt256 mm (10rsquorsquo) 15
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 62 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 8 Sand 006-2 mm (gritty) 0 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Unnamed Tributary STATION HLR-4 STREAM CLASS LAT N 39 13035 (upstream) LONG W 078 05129 (upstream) LAT N 39 13000 (downstream) LONG W 078 05086 (downstream) FORM COMPILED BY Marie Dahl RIVER BASIN Potomac River INVESTIGATORS M Dahl HKepple SRobertson AFisher
DATE 31 May 2008 TIME 5 PM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality 2008
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) YES x NO
rain (steady rain) Air Temperature __22___degC showers (intermittent) Comments _______________________
_0__ cloud cover _0__ ______________________________
x clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area __-___ km2 mi2 Montane Significant springs andor seeps x Valley Marsh or swamp Montane amp Valley Mixture of origins Other ___________________
COMMENTS Snowden Bridge development (upstream) and sewer line
i
j
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence x FieldPasture Industrial Obvious sources Agricultural Other ____________ Construction upstream and adjacent x Residential Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left Forested with rocky banks field across silt fence and 2m tree strip Right Forested with some rocky banks and forest through the highway Indicate the dominant vegetation type(s) and record the dominant species
x Trees x Shrubs x Grasses Forbs Dominant species White Ash and Hackberry
INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width ___38__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area __380__m2
Estimated stream depth __12__cm Proportion of Reach Represented by Stream High water mark __2___m Morphology Types Surface velocity (at thalweg) __46___msec x Riffle__25___ x Run__70___ x Pool__5___ Channelized Yes x No Dam Present x Yes No
LARGE WOODY DEBRIS
LWD _______ m2 Density of LWD _______ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation ___NA____ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present___Algae___
WATER QUALITY Temperature __16___degC Water Odors x NormalNone Sewage Specific Conductance __-____ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ___-_____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used ____NA_____ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells x Other___Shale_______ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 0 Detritus sticks wood coarse plant materials
10 Boulder gt256 mm (10rsquorsquo) 5
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 45 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 30 Sand 006-2 mm (gritty) 0 Marl grey shell
fragments 0
Silt 0004-006 mm 20 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Lick Run STATION HLR-5 STREAM CLASS LAT N 39 127517 (upstream) LONG W 078 05685(upstream) LAT N 39 12784 (downstream) LONG W 078 05631 (downstream) FORM COMPILED BY B Millholland RIVER BASIN Potomac River INVESTIGATORS B Millholland JTovar SRobertson MDahl WBousquet JPangle
DATE 29 May 2008 TIME 1040 AM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project
k
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature __15___degC showers (intermittent) Comments _______________________
_5_ X cloud cover _0__ ______________________________
x clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane Significant springs andor seeps x Valley Marsh or swamp Montane amp Valley Mixture of origins Other ___________________
COMMENTS Lick Run appears to be intermittent and may be dry in Fall 2008
l
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence x FieldPasture Industrial Obvious sources Agricultural Other ____________ Adjacent logging stream bank scour Residential Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left 85 young deciduous forest 15 logged Bank=Soil vegetation and cobbles Right Young deciduous forest on 30 slope Bank= Soil vegetation and cobble Indicate the dominant vegetation type(s) and record the dominant species
x Trees Shrubs Grasses Forbs Dominant species Mixed Deciduous INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width __11__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area 110 m2
Estimated stream depth _009__m Proportion of Reach Represented by Stream High water mark __96___m Morphology Types Surface velocity (at thalweg) __01___msec x Riffle__30___ x Run__5___ x Pool__65___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___0____ m2 Density of LWD ___-____ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __70___ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present__________________________________________________________
WATER QUALITY Temperature __14___degC Water Odors x NormalNone Sewage Specific Conductance __-__ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ___-___ Water Surface Oils Slick Sheen Globs Flecks pH __-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used _____NA______ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other_____None_____
SEDIMENT AND SUBSTRATE
Odors Deposits Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other________________ x Other Slightly Salty Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 0 Detritus sticks wood coarse plant materials
5 Boulder gt256 mm (10rsquorsquo) 0
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 75 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 23 Sand 006-2 mm (gritty) 2 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION McCann Farm STATION HLR-6 STREAM CLASS LAT N 39 13076 (upstream) LONG W 078 06539 (upstream) LAT N 39 13063 (downstream) LONG W 078 06473 (downstream) FORM COMPILED BY MDahl RIVER BASIN Potomac River INVESTIGATORS MDahl JPangle SRobertson W Bousqet BMillholland JTovar H Kepple
DATE 30 May 2008 TIME 1050 AM (start)
PROJECT NAME
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) YES x NO
rain (steady rain) Air Temperature __18___degC showers (intermittent) Comments _______________________
_85_x cloud cover _10__ ______________________________
clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x (Significant springs andor seeps) x ( Valley) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS Multi-habitat sampling with D-frame nets and seine
m
n
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial x No evidence Some evidence x FieldPasture Industrial Obvious sources x Agricultural Other ____________ Minor stream bank scour Residential Local Watershed Erosion x None Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left 35 bank grasses scattered trees and some bushes Right High bank with grasses and scattered trees Indicate the dominant vegetation type(s) and record the dominant species
x Trees Shrubs Grasses Forbs Dominant species Black Walnut and Festuca elatior
INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width __2___m Fully open Partly open x Partly shaded Fully shaded Sampling reach area __200_m2
Estimated stream depth __03_m Proportion of Reach Represented by Stream High water mark __1___m Morphology Types Surface velocity (at thalweg) __166_msec x Riffle__0___ x Run__55___ x Pool__45___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___2____ m2 Density of LWD ___-____ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __20_____ x Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present ____Glyceria (grass)sp__________
WATER QUALITY Temperature __16___degC Water Odors x NormalNone Sewage Specific Conductance ___-___ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ____-____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used ______NA___ Clear x Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other__NONE__ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 0 Detritus sticks wood coarse plant materials
5 Boulder gt256 mm (10rsquorsquo) 0
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 27 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 38 Sand 006-2 mm (gritty) 5 Marl grey shell
fragments 0 (lt1)
Silt 0004-006 mm 25 Clay lt 0004 mm (slick) 5 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Appendix B
WATER QUALITY ANALYSIS THROUGH THE VIRGINIA
STREAM CONDITION INDEX (VSCI)
The Virginia Stream Condition Index (VSCI) relies on eight metrics to categorize stream health According to Burton and Gerritsen (2003 p 1-1) this index ldquohellip can be used as a primary indicator of ecosystem health and can identify impairment with respect to the reference (or natural) conditionrdquo These metrics are numeric measures of a biological communityrsquos species composition diversity ecological niches or tolerance to pollution
Table 13 on the next page describes each VSCI metric lists its formula and provides the best standard value (BSV) for that metric Based on a 100-point scale the BSV is the score that reflects optimum conditions for aquatic life ie the best water quality value possible (DEQ 2006b pp 8 33) The table also explains whether the score increases or decreases with environmental stress
As Table 13 indicates each of the eight VSCI metrics is calculated individually Each formula provides a unitless score based on a 100-point scale with 0 as the worst possible score and 100 as the best Any metric that provides a result above 100 is changed to 100 in order for the index to work properly After the eight metrics are calculated they are summed and averaged to provide a final VSCI score on a 100-point scale
Based on statistical analysis of Virginiarsquos streams and their BMI communities the DEQ (2006b p 8) currently uses the following tiers to interpret VSCI scores
VSCI Scores Aquatic Life Use
(ALI) Tiers
lt 42 severely stressed
42-55 moderately stressed
55-63 unknownfair (gray zone)
63-73 good
gt73 excellent
Section 2 of this report presents the results of calculations for the six BMI sampling sites the research team studied in the Hiatt-Lick Run subwatershed in 2008
o
p
1 Total taxa the number of distinct taxa in the sample measures the overall diversity of the macroinvertebrate assemblage The best standard value (BSV) is 189 Score = 100 x (X189) Total taxa decreases with stress 2 EPT taxa the sum of the distinct taxa in the orders Ephemeroptera (mayflies) Plecoptera (stoneflies) and Trichoptera (caddisflies) These orders are known to be sensitive to pollution The best standard value (BSV) is 117 Score = 100 x (X117) EPT taxa decreases with stress 3 Percent Ephemeroptera the percent abundance of individuals in the sample that belong to the order Ephemeroptera (mayflies) The best standard value (BSV) is 477 Score = 100 x (X477) Percent Ephemeroptera decreases with stress 4 Percent Plecoptera + Trichoptera less Hydropsychidae the percent abundance of individuals in the sample that belong to the orders Plecoptera (stoneflies) and Trichoptera (caddisflies) excluding the pollution-tolerant caddisfly family Hydropsychidae The best standard value (BSV) is 562 Score = 100 x (X562) Percent Plecoptera + Trichoptera less Hydropsychidae decreases with stress 5 Percent Scrapers the percent abundance of individuals in the sample whose primary functional mechanism for feeding is grazing on the substrate or periphyton (ie attached algae and associated material) The best standard value (BSV) is 467 Score = 100 x (X467) Percent scrapers decreases with stress 6 Percent Chironomidae the percent abundance of individuals in the sample that are Chironomidae (non-biting midge) larvae and pupae The best standard value (BSV) is 16 Score = 100 x [(100-X)(100-16)] Percent Chironomidae increases with stress 7 Percent Top Two Dominant the percent abundance of the two most frequent taxa present in the sample The best standard value (BSV) is 328 Score = 100 x [(100-X)(100-328)] The Percent Top 2 Dominant increases with stress 8 Hilsenhoff Biotic Index (HBI) an abundance-weighted average pollution tolerance of all the individuals within a sample keyed to the family level Tolerance ratings range from 1 (highly intolerant) to 10 (highly tolerant) The best standard value (BSV) is 29 Score = 100 x [(10-X)(10-29)] The Hilsenhoff Biotic Index (HBI) increases with stress ______________________________________________________________________________ Table 13 Description of Benthic Macroinvertebrate VSCI Metrics Includes responses (increases decreases) of each VSCI metric to degraded water quality conditions Adapted from Burton and Gerritsen (2003 pp 3-15 3-16 6-2) and DEQ (2008)
q
Appendix C
LABORATORY BENCH SHEETS
Shenandoah Universityrsquos Environmental Studies Department developed the Benthic Macroinvertebrate Laboratory Bench Sheet and VSCI Worksheet to record BMI specimen identifications and their VSCI classifications for each sample
This form is based in part on bench sheets developed by the EPA and Virginia DEQ for recording specimen identifications Additional columns on Shenandoah Universityrsquos forms for relative numbers and VSCI classifications make the calculation and verification of VSCI scores easier
Note the column for taxonomistrsquos initials Specimens were identified by students in Shenandoah Universityrsquos research group and then verified or where necessary changed by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality This external review helps assure the validity of the study Originals of these bench sheets with handwritten taxonomistsrsquo initials will be kept on file at Shenandoah Universityrsquos Environmental Studies Department for five years after the completion of this study
r
s
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-1 STATION NAME Mouth LAT N 39deg 12749rsquo (upstream) LONG W 078deg 04209rsquo (upstream) LAT N 39deg 12729rsquo (downstream) LONG W 078deg 04148rsquo (downstream) FORM COMPILED BY Jeremy Tovar W S Bousquet RIVER BASIN Potomac River INVESTIGATORS Marie Dahl Brandon Millholand J W Pangle Sean Robertson Jeremy Tovar Woodward Bousquet Ben Bousquet
Collection date 21 May 2008 Sheet completion date 12 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Hirudinea 3 33 JT BVW PR 8 SP Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Coenagrionidae 2 22 BVW PR 9 CR Ephemeroptera (mayflies) Caenidae 1 11 JT BVW CG 6 SP Ephemerllidae 4 44 JT BVW CG 2 CR Heptageniidae 5 55 JT BVW SC 3 CG Isonychiidae 1 11 BVW CF 3 CG Plecoptera (stoneflies) Perlidae 14 154 JT BVW PR 2 CR Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Corydalidae 1 11 JT BVW PR 5 CR Coleoptera (beetles) Elmidae 24 264 I JT BVW SC 4 CG Dytiscidae 4 44 JT BVW PR 6 GN Psephenidae 3 33 JT BVW SC 4 CG Diptera (true flies) Tipulidae 3 33 JT BVW SH 4 BU Chironomidae 26 286 JT BVW CG 6 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
t
Other Total Individuals 91 1001 Total Taxa 13
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
u
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-1 STATION NAME Mouth LAT N 39deg 12749rsquo (upstream) LONG W 078deg 04209rsquo (upstream) LAT N 39deg 12729rsquo (downstream) LONG W 078deg 04148rsquo (downstream) FORM COMPILED BY Jeremy Tovar W S Bousquet RIVER BASIN Potomac River INVESTIGATORS Brandon Millholand JW Pangle Jeremy Tovar Heather Kepple
Collection date 10 October 2008 Sheet completion date 12 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Oligochaeta 3 35 JT BVW CG 8 BU Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Coenagrionidae 2 23 JT BVW PR 9 CR Ephemeroptera (mayflies) Heptageniidae 12 140 JT BVW SC 3 CG Isonychiidae 2 23 BVW CF 3 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Hydropsychidae 16 186 JT BVW CF 6 CG Philopotamidae 31 360 JT BVW CF 2 CG Hemiptera (true bugs) (Veliidae) (5) - JT BVW ( ) Megaloptera (dobsonflies etc) Corydalidae 10 116 JT BVW PR 5 CR Coleoptera (beetles) Dytiscidae 1 12 JT BVW PR 6 GN Psephenidae 7 81 JT BVW SC 4 CG Diptera (true flies) Tipulidae 2 23 BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
v
Other Total Individuals 86
(91) 999
Total Taxa 10 (11)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009 Veliidae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
w
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-2 STATION NAME Package Plant LAT N 39deg 12958rsquo (upstream) LONG W 078deg 04900 (upstream) LAT N 39deg 12958rsquo (downstream) LONG W 078deg 04822 (downstream) FORM COMPILED BY JW Pangle W Bousquet RIVER BASIN Potomac River INVESTIGATORS JW Pangle S Robertson J Tovar M Dahl B Millholland W Bousquet
Collection date 29 May 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Plecoptera (stoneflies) Perlidae 11 136 I JP BVW PR 1 CR Trichoptera (caddisflies) Hydropsychidae 10 123 I JP BVW CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 14 173 I JP BVW SC 4 CG Diptera (true flies) Chironomidae 32 395 I JP BVW CG 6 BU Simuliidae 14 173 I JP BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
x
Other Total Individuals 81 1000 Total Taxa 5
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-2 STATION NAME Package Plant LAT N 39deg 12958rsquo (upstream) LONG W 078deg 04900 (upstream) LAT N 39deg 12958rsquo (downstream) LONG W 078deg 04822 (downstream) FORM COMPILED BY JW Pangle W Bousquet RIVER BASIN Potomac River INVESTIGATORS JW Pangle Brandon Millholland Jeremy Tovar Heather Kepple
Collection date 10 October 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Heptageniidae 40 392 I WBJP SC 3 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Hydropsychidae 7 69 I JP WB BVW CF 6 CG Philopotamidae 28 275 I JP WB BVW CF 2 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Corydalidae 6 59 I JP WB BVW PR 5 CR Coleoptera (beetles) Psephenidae 7 69 I JP WB BVW SC 4 CG Elmidae 8 78 I JP WB BVW SC 4 CG Diptera (true flies) Tipulidae 6 59 I JP WB BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
y
z
Other Total Individuals 102 1001 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-3 STATION NAME Rt 664 Bridge LAT N 39deg 12934rsquo (upstream) LONG W 078deg 05019rsquo (upstream) LAT N 39deg 12951rsquo (downstream) LONG W 078deg 04958rsquo (downstream) FORM COMPILED BY S Robertson W Bousquet RIVER BASIN Potomac River INVESTIGATORS SRobertson BMillholland WBousquet MDahl JPangle
Collection date 28 May 2008 Sheet completion date 11 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Leptophlebiidae 1 15 I SGR BVW CG 2 CR Baetidae 1 15 I BVW CG 5 CG Plecoptera (stoneflies) Perlidae 3 46 I SGR BVW PR 1 CR Nemouridae 1 15 I BVW SH 3 CR Trichoptera (caddisflies) Hydropsychidae 5 77 I SGR BVW CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 27 415 I SGR BVW SC 4 CG Psephenidae 2 31 A SGR BVW SC 4 CG Diptera (true flies) Simuliidae 5 77 I SGR BVW CF 6 CG Chironomidae 20 308 I SGR BVW CG 6 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
aa
bb
Other Total Individuals 65 999 Total Taxa 9
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-3 STATION NAME Rt 664 Bridge LAT N 39deg 12934rsquo (upstream) LONG W 078deg 05019rsquo (upstream) LAT N 39deg 12951rsquo (downstream) LONG W 078deg 04958rsquo (downstream) FORM COMPILED BY S Robertson RIVER BASIN Potomac River INVESTIGATORS S Robertson M Dahl A Fisher H Kepple
Collection date 10 Oct 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Heptageniidae 2 20 I SGR SC 3 CG Isonychiidae 2 20 I BVW CF 2 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Philopotamidae 36 367 I SGR CF 2 CG Hydropsychidae 47 480 I SGR CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 2 20 A SGR SC 4 CG Psephenidae 8 82 A SGR SC 4 CG Diptera (true flies) Tabanidae 1 10 I SGR PR 7 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
cc
dd
Other Total Individuals 98 999 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-4 STATION NAME Unnamed Tributary LAT (upstream) N 39deg 13035rsquo LONG (upstream) W 078deg 05129rsquo LAT (downstream) N 39deg 13000rsquo LONG (downstream) W 078deg 5086rsquo FORM COMPILED BY A Fisher M Dahl rev W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson A Fisher H Kepple
Collection date 31 May 2008 Sheet completion date rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Flatworms (Turbellaria) Worms (Oligochaeta) Naididae 1 14 - BVW CG 8 BU Leeches (Hirudinea) Snails (Gastropoda) Clams (Bivalvia) Isopods (Isopoda) Asellidae 3 42 A AF BVW CG 8 SP Scuds (Amphipoda) Crayfish Shrimp (Decapoda) Water Mites (Hydracarina) Dragonflies (Odonata Anisoptera) Damselflies (Odonata Zygoptera) Mayflies (Ephemeroptera) Stoneflies (Plecoptera) Perlidae 2 28 I AF BVW PR 1 CR Caddisflies (Trichoptera) True Bugs (Hemiptera) Corixidae 1 14 I AF BVW MP 9 DV Dobsonflies (Megaloptera) Beetles (Coleoptera) Dytiscidae 1 14 I AF BVW PR 6 GN True Flies (Diptera) Chironomidae 61 847 I AF BVW CG 6 BU Simuliidae 3 42 I AF BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
ee
ff
Other Total Individuals 72 1001 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-4 STATION NAME Unnamed Tributary LAT (upstream) N 39deg 13035rsquo LONG (upstream) W 078deg 05129rsquo LAT (downstream) N 39deg 13000rsquo LONG (downstream) W 078deg 5086rsquo FORM COMPILED BY Andrew G M Fisher W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson A Fisher H Kepple
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Flatworms (Turbellaria) Worms (Oligochaeta) Lumbriculidae 2 32 A AF BVW CG 8 BU Leeches (Hirudinea) Snails (Gastropoda) Lymnaeidae 1 16 A BVW CG 9 GN Clams (Bivalvia) Isopods (Isopoda) Scuds (Amphipoda) Crayfish Shrimp (Decapoda) Cambaridae 1 16 A AF BVW GN 5 GN Water Mites (Hydracarina) Dragonflies (Odonata Anisoptera) Damselflies (Odonata Zygoptera) Calopterygidae 4 63 I BVW PR 7 CL Mayflies (Ephemeroptera) Tricorythidae 1 16 I BVW CG 5 SP Stoneflies (Plecoptera) Caddisflies (Trichoptera) Phryganeidae 4 63 I AF BVW SH 4 CR Hydropsychidae 6 95 I AF BVW CF 6 CG I True Bugs (Hemiptera) Corixidae 8 127 I AF BVW MP 9 DV (Veliidae ) (7) - I AF BVW ( ) Dobsonflies (Megaloptera) Sialidae 3 48 I AF BVW PR 7 SP Corydalidae 2 32 I AF BVW PR 5 CR Beetles (Coleoptera) Haliplidae 1 16 A AF BVW MP 8 GN Dysticidae 7 111 I AF BVW PR 6 GN Hydrophilidae 1 16 A AF BVW PR 8 GN Elmidae 3 48 I BVW SC 4 CG True Flies (Diptera) Chironomidae 8 127 I AF BVW CG 6 BU Simuliidae 7 111 I AF BVW CF 6 CG Tipulidae 4 63 I AF BVW SH 4 GU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
gg
hh
Other Total Individuals 63
(70) 1000
Total Taxa 17 (18)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Veliidae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-5 STATION NAME Lick Run LAT N 39deg 12752rsquo (upstream) LONG W 078deg 05685rsquo (upstream) LAT N 39deg 12784rsquo (downstream) LONG W 078deg 05631rsquo (downstream) FORM COMPILED BY Brandon Millholland W Bousquet RIVER BASIN Potomac River INVESTIGATORS J Pangle S Robertson W Bousquet JTovar M Dahl
Collection date 29 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Gomphidae 1 15 I BVW PR 3 BU Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Leptophlebiidae 11 167 A LBM BVW CG 2 CR Plecoptera (stoneflies) Perlidae 10 152 A LBM BVW PR 1 CR Perlodiae 1 15 A LBM BVW PR 2 CR Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 5 76 A LBM BVW SC 4 CG Elmidae 2 30 I LBM BVW SC 4 CG Diptera (true flies) (Culicidae) (3) - P LMB BVW ( ) Chironomidae 23 348 I LBM BVW CG 6 BU Simuliidae 13 197 I LBM BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
ii
jj
Other Total Individuals 66
(69) 1000
Total Taxa 7 (8)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Culicidae (Diptera) pupae not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl J Pangle S Robertson W Bousquet B Millholland J Tovar H Kepple
Collection date 30 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 1 12 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Asellidae 114 965 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Plecoptera (stoneflies) Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 2 23 A MRD BVW SC 4 CG Diptera (true flies)
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
kk
ll
Other Total Individuals 117 1000 Total Taxa 3
Notes Subsample taken 29 October 2009 All taxonomic determinations by MRD were confirmed by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson K McCann A Fisher H Kepple A Barrett
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 2 17 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Sphaeriidae 2 17 I MRD BVW CF 8 BU Isopoda (isopods) Asellidae 99 853 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Calopterygidae 2 17 A MRD BVW PR 7 CL Ephemeroptera (mayflies) Leptophlebiidae 2 17 I MRD BVW CG 2 CR Heptageniidae 4 34 A MRD BVW SC 3 CG Plecoptera (stoneflies) Perlidae 1 09 A MRD BVW PR 1 CR Trichoptera (caddisflies) Hemiptera (true bugs) (Gerridae) (1) - A MRD BVW ( ) Corixidae 1 09 A MRD BVW PR 5 CR Megaloptera (dobsonflies etc) Coleoptera (beetles) Dryopidae 1 09 A MRD BVW SC 5 CG Diptera (true flies) Tipulidae 2 17 I BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
mm
nn
Other Total Individuals 116
(117) 999
Total Taxa 10 (11)
Notes Subsample taken 6 November 2008 Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009 Gerridae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
- Disclaimer ii
- Title Page iii
- Acknowledgements v
- Table of Contents vii
- SECTION 2 RESULTS DATA TABLES 7
- OVERVIEW AND FINDINGS
- Introduction
- RESULTS DATA TABLES
- REFERENCES
- FIELD DATA SHEETS
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- SubstrateType
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- WATER QUALITY ANALYSIS
- THROUGH THE VIRGINIA
- STREAM CONDITION INDEX (VSCI)
- LABORATORY BENCH SHEETS
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
Site HLR-5 Lick Run
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 7 189 3702 EPT taxa 3 117 2563 Ephemeroptera 167 477 3504 Plec +Tric less Hydropsych 167 562 2975 Scrapers 106 467 2276 Chironomidae 348 16 6637 Top 2 Dominant 545 328 6778 Hilsenhoff Biotic Index (HBI) 43 29 803VSCI Score 455
According to the DEQrsquos water quality standards aquatic life at this site is moderately stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 10 VSCI Scores for Lick Run (HLR-5) Site in Hiatt-Lick Run Sampled 29 May 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
Note
Water conditions at the Lick Run (HLR-5) site were too low in October 2008 to permit a sample to be taken
11
Site HLR-6 McCann Farm
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 3 189 1592 EPT taxa 0 117 003 Ephemeroptera 00 477 004 Plec +Tric less Hydropsych 00 562 005 Scrapers 23 467 496 Chironomidae 00 16 10007 Top 2 Dominant 988 328 188 Hilsenhoff Biotic Index (HBI) 79 29 296VSCI Score 190
According to the DEQrsquos water quality standards aquatic life at this site is severely stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 11 VSCI Scores for McCann Farm (HLR-6) Site in Hiatt-Lick Run Sampled 30 May 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 10 189 5292 EPT taxa 3 117 2563 Ephemeroptera 51 477 1074 Plec +Tric less Hydropsych 09 562 165 Scrapers 43 467 926 Chironomidae 00 16 10007 Top 2 Dominant 887 328 1688 Hilsenhoff Biotic Index (HBI) 75 29 352VSCI Score 315
According to the DEQrsquos water quality standards aquatic life at this site is severely stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 12 VSCI Scores for McCann Farm (HLR-6) Site in Hiatt-Lick Run Sampled 10 October 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
12
REFERENCES
Barbour Michael T Jeroen Gerritsen Blaine D Snyder and James B Stribling 1999 Rapid bioassessment protocols for use in streams and wadeable rivers periphyton benthic macroinvertebrates and fish Second edition EPA 841-B-99-002 Washington DC US Environmental Protection Agency Office of Water Available online at httpwwwepagovOWOWmonitoringbioasspshtml Burton June and Jeroen Gerritsen 2003 A stream condition index for Virginia non-coastal streams Prepared for the US EPA Office of Science and Technology US EPA Region 3 Environmental Services Division and Virginia Department of Environmental Quality Owings Mills Maryland Tetra Tech Inc Available online at httpwwwdeqvirginiagovwatermonitoringpdf vastrmconpdf Hessler Rachel A Andrea R Martin Heidi D Ruggeri William B Seymour Jr and Woodward S Bousquet 2006 Water quality assessment using benthic macroinvertebrates A study and compilation Winchester Virginia Environmental Studies Program Shenandoah University Occasional publication no 10 Igoe Robert 2006 DEQ identifies nine impaired streams in Clarke Frederick The Winchester Star 15 July B1 Jackson Charlie 2005 Survey targets Opequon cleanup The Winchester Star 19 August B1 Johnston Elizabeth A and Woodward S Bousquet 2003 What happens when it rains A study of water quality in Abrams Creek and Town Run Winchester and Frederick County Virginia Winchester Virginia Environmental Studies Program Shenandoah University Occasional publication no7 Merritt Richard W and Kenneth W Cummins 1996 An introduction to the aquatic insects of North America Dubuque Iowa KendallHunt Publishing Company Opequon Creek IP Steering Committee Virginia Tech Department of Biological Systems Engineering and Center for TMDL and Watershed Studies University of Virginia Institute for Environmental Negotiation and West Virginia University Division of Resource Management 2006 Opequon Creek watershed TMDL implementation plan Blacksburg Virginia Virginia Tech Department of Biological Systems Engineering Available online at httpwwwtmdlbsevteduoutreachC82 Pennak Robert W 1953 Fresh-water invertebrates of the United States New York New York The Ronald Press Company Rosenburg David M and Vincent H Resh 1993 Freshwater biomonitoring and benthic macroinvertebrates New York New York Chapman amp Hall Shenandoah University Public Relations Office 2002 Data collected by SU and its partners helps identify impaired water Winchester Virginia Available online at httpwwwsuedutemp_ newscfmurlnum=32 Thorp James H and Alan P Covich 1991 Ecology and classification of North American freshwater invertebrates San Diego California Academic Press Inc
13
US Environmental Protection Agency 1997 Volunteer stream monitoring a methods manual EPA 841-B-97-003 Washington DC US Environmental Protection Agency Office of Water Van Meter Val 2003 Opequon Creek pollution limits to be established The Winchester Star 4 April B1 Virginia Department of Environmental Quality 2006a Virginia 305(b)303(d) water quality integrated report to Congress and the EPA Administrator for the period January 1 2000 to December 31 2004 Richmond Virginia Virginia Department of Environmental Quality Available online at httpwwwdeqvirginiagovwqair2006html __________ 2006b Using probabilistic data to validate the non-coastal Virginia Stream Condition Index (draft) VDEQ technical bulletin WQA2006-001 Virginia Department of Environmental Quality Available online at httpwwwloudounwatershedorgstream_assessmentreference_documents VA_DEQ_SCI_Validationpdf __________ 2008 Biological monitoring program quality assurance project plan for wadeable streams and rivers Richmond Virginia Virginia Department of Environmental Quality Update available online at httpwwwdeqstatevausbiomonitoringdocumentsBioMonQAPP_13Aug2008pdf Virginia Tech Aquatic Entomology Laboratory 2005 Taxa dictionary [Excel file] Blacksburg Virginia Virginia Tech Department of Biological Systems Engineering 2003a Opequon watershed TMDLs for benthic impairments Abrams Creek and Lower Opequon Creek Frederick and Clarke Counties Virginia Prepared for the Virginia Department of Environmental Quality and Virginia Department of Conservation and Recreation Blacksburg Virginia Available online at httpwwwdeqstate vaustmdlapptmdlsshenrvrabropebcpdf Voshell J Reese Jr 2002 A guide to common freshwater invertebrates of North America Blacksburg Virginia The McDonald amp Woodward Publishing Company
14
APPENDICES
15
16
a
Appendix A
FIELD DATA SHEETS
This appendix presents a Site Characterization Field Data Sheet for each of the six sampling reaches established on Hiatt Run and Lick Run for this study They were filled out during the sampling runs made in May 2008
These forms were adapted by Shenandoah University from forms used by the EPA and Virginia DEQ for recording characteristics of sampling sites
b
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Mouth STATION _HLR-1_____ STREAM CLASS LAT N 39 12749 (upstream) LONG W 078 04209(upstream) LAT N 39 12729 (downstream) LONG W 078 04148 (downstream) FORM COMPILED BY Jeremy Tovar RIVER BASIN Potomac River INVESTIGATORS JPangle B Millholland MDahl SRobertson W Bousquet JTovar BBousquet
DATE May 21 2008_________ TIME_250 PM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature __24___degC showers (intermittent) Comments _______________________
___ cloud cover _0_ ______________________________
X clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x ( Significant springs andor seeps) x (Valley ) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS
c
d
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence x FieldPasture Industrial Obvious sources Agricultural Other ____________ Stream scour steep slope erosion x Residential Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left Field and young thin deciduous forest 100 Bank=Grass cobble and shrub Right 90 maturing deciduous forest 45 shale slope Bank=Bedrock soil and herbs Indicate the dominant vegetation type(s) and record the dominant species
x Trees Shrubs x (Grasses) Forbs Dominant species Oak species and mix deciduous
INSTREAM FEATURES
Measured reach length ___100__m Canopy Cover Estimated stream width _35__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area __350_m2
Estimated stream depth _008_m Proportion of Reach Represented by Stream High water mark _15_m Morphology Types Surface velocity (at thalweg) _05__msec x Riffle__75___ x Run__20___ x Pool__5___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___00__ m2 Density of LWD _-_ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __15_____ x Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present Algae
WATER QUALITY Temperature __19___degC Water Odors x NormalNone Sewage Specific Conductance - Petroleum Chemical Fishy Other___________________ Dissolved Oxygen - Water Surface Oils Slick Sheen Globs Flecks pH __-_ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used _____NA________ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic x None Relict shells Other______None_____ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 8 Detritus sticks wood coarse plant materials
0 Boulder gt256 mm (10rsquorsquo) 0
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 51 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 40 Sand 006-2 mm (gritty) 1 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University
SITE CHARACTERIZATION FIELD DATA SHEET FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Package Plant STATION HLR-2 STREAM CLASS LAT N 39 129580 (upstream) LONG W 078 04900 (upstream) LAT N 39 129581 (downstream) LONG W 078 04822 (downstream) FORM COMPILED BY JW Pangle RIVER BASIN Potomac River INVESTIGATORS JPangle JTovar MDahl B Millholland SRobertson W Bousquet
DATE__19 May 2008_ TIME__1240__ PM
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature _____degC showers (intermittent) Comments __Sunny Day_____________
_5__ cloud cover _0__ ______________________________
x clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x (Significant springs andor seeps) x (Valley) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS
e
f
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence FieldPasture Industrial Obvious sources Agricultural Other ____________ __Upstream Construction and Stream bank Scour Residential __________________________________ Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left Deciduous forest with thick herb and shrub layers bank= vegetation and rocks Right Deciduous forest with thick herb and shrub layers bank=vegetation soil and rocks Indicate the dominant vegetation type(s) and record the dominant species
x Trees x Shrubs Grasses Forbs Dominant species Black Walnut White Ash Hackberry and American Sycamore
INSTREAM FEATURES
Measured reach length 100 m Canopy Cover Estimated stream width _35__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area __350_m2
Estimated stream depth __15__m Proportion of Reach Represented by Stream High water mark __22___m Morphology Types Surface velocity (at thalweg) _035__msec x Riffle__30___ x Run__30___ x Pool__40___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___10____ m2 Density of LWD _______ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __30___ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present__________________________________________________________
WATER QUALITY Temperature __18___degC Water Odors x NormalNone Sewage Specific Conductance __-____ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ____-____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used _____NA____ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other___None_____ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach SubstrateType Characteristic
Composition in Sampling Reach
Bedrock 3 Detritus sticks wood coarse plant materials
2 Boulder gt256 mm (10rsquorsquo) 5
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 70 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 22 Sand 006-2 mm (gritty) 0 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiattrsquos Run LOCATION Rt 664 Bridge STATION HLR-3 STREAM CLASS LAT N 39 12934 (upstream) LONG W 078 05019 (upstream) LAT N 39 12958 (downstream) LONG W 078 04958 (downstream) FORM COMPILED BY Sean Robertson RIVER BASIN Potomac River INVESTIGATORS SRobertson BMillholland WBousquet MDahl JPangle
DATE 28 May 2008 TIME 1127 AM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality 2008
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature __14___degC showers (intermittent) x Trace Comments _______________________
_65 x cloud cover ___ ______________________________
x clearsunny __________________________________
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x (Significant springs andor seeps) x (Valley) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS
g
h
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence Some evidence x FieldPasture Industrial x Obvious sources Agricultural x Other Old Package Plant Downstream x Residential Bridge and Road Power line and Pump station Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left 70 field 30 shrubs and deciduous trees Bank=Soil and vegetation Right Maturing deciduous forest steep slope Bank=Shale Bedrock Indicate the dominant vegetation type(s) and record the dominant species
x Trees x Shrubs x Grasses Forbs Dominant species Sycamore and Red Oaks
INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width __51___m Fully open x Partly open Partly shaded Fully shaded Sampling reach area _510_m2
Estimated stream depth __135___m Proportion of Reach Represented by Stream High water mark __-___m Morphology Types Surface velocity (at thalweg) __20___msec x Riffle_40_ x Run_50_ x Pool 10 Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___0____ m2 Density of LWD ___-____ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation ___50____ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present Brown Algae
WATER QUALITY Temperature __17___degC Water Odors x NormalNone Sewage Specific Conductance ___-___ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ___-_____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used ____NA_______ Clear x Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other____None____ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 15 Detritus sticks wood coarse plant materials
0 Boulder gt256 mm (10rsquorsquo) 15
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 62 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 8 Sand 006-2 mm (gritty) 0 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Unnamed Tributary STATION HLR-4 STREAM CLASS LAT N 39 13035 (upstream) LONG W 078 05129 (upstream) LAT N 39 13000 (downstream) LONG W 078 05086 (downstream) FORM COMPILED BY Marie Dahl RIVER BASIN Potomac River INVESTIGATORS M Dahl HKepple SRobertson AFisher
DATE 31 May 2008 TIME 5 PM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality 2008
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) YES x NO
rain (steady rain) Air Temperature __22___degC showers (intermittent) Comments _______________________
_0__ cloud cover _0__ ______________________________
x clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area __-___ km2 mi2 Montane Significant springs andor seeps x Valley Marsh or swamp Montane amp Valley Mixture of origins Other ___________________
COMMENTS Snowden Bridge development (upstream) and sewer line
i
j
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence x FieldPasture Industrial Obvious sources Agricultural Other ____________ Construction upstream and adjacent x Residential Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left Forested with rocky banks field across silt fence and 2m tree strip Right Forested with some rocky banks and forest through the highway Indicate the dominant vegetation type(s) and record the dominant species
x Trees x Shrubs x Grasses Forbs Dominant species White Ash and Hackberry
INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width ___38__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area __380__m2
Estimated stream depth __12__cm Proportion of Reach Represented by Stream High water mark __2___m Morphology Types Surface velocity (at thalweg) __46___msec x Riffle__25___ x Run__70___ x Pool__5___ Channelized Yes x No Dam Present x Yes No
LARGE WOODY DEBRIS
LWD _______ m2 Density of LWD _______ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation ___NA____ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present___Algae___
WATER QUALITY Temperature __16___degC Water Odors x NormalNone Sewage Specific Conductance __-____ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ___-_____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used ____NA_____ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells x Other___Shale_______ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 0 Detritus sticks wood coarse plant materials
10 Boulder gt256 mm (10rsquorsquo) 5
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 45 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 30 Sand 006-2 mm (gritty) 0 Marl grey shell
fragments 0
Silt 0004-006 mm 20 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Lick Run STATION HLR-5 STREAM CLASS LAT N 39 127517 (upstream) LONG W 078 05685(upstream) LAT N 39 12784 (downstream) LONG W 078 05631 (downstream) FORM COMPILED BY B Millholland RIVER BASIN Potomac River INVESTIGATORS B Millholland JTovar SRobertson MDahl WBousquet JPangle
DATE 29 May 2008 TIME 1040 AM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project
k
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature __15___degC showers (intermittent) Comments _______________________
_5_ X cloud cover _0__ ______________________________
x clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane Significant springs andor seeps x Valley Marsh or swamp Montane amp Valley Mixture of origins Other ___________________
COMMENTS Lick Run appears to be intermittent and may be dry in Fall 2008
l
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence x FieldPasture Industrial Obvious sources Agricultural Other ____________ Adjacent logging stream bank scour Residential Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left 85 young deciduous forest 15 logged Bank=Soil vegetation and cobbles Right Young deciduous forest on 30 slope Bank= Soil vegetation and cobble Indicate the dominant vegetation type(s) and record the dominant species
x Trees Shrubs Grasses Forbs Dominant species Mixed Deciduous INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width __11__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area 110 m2
Estimated stream depth _009__m Proportion of Reach Represented by Stream High water mark __96___m Morphology Types Surface velocity (at thalweg) __01___msec x Riffle__30___ x Run__5___ x Pool__65___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___0____ m2 Density of LWD ___-____ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __70___ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present__________________________________________________________
WATER QUALITY Temperature __14___degC Water Odors x NormalNone Sewage Specific Conductance __-__ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ___-___ Water Surface Oils Slick Sheen Globs Flecks pH __-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used _____NA______ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other_____None_____
SEDIMENT AND SUBSTRATE
Odors Deposits Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other________________ x Other Slightly Salty Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 0 Detritus sticks wood coarse plant materials
5 Boulder gt256 mm (10rsquorsquo) 0
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 75 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 23 Sand 006-2 mm (gritty) 2 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION McCann Farm STATION HLR-6 STREAM CLASS LAT N 39 13076 (upstream) LONG W 078 06539 (upstream) LAT N 39 13063 (downstream) LONG W 078 06473 (downstream) FORM COMPILED BY MDahl RIVER BASIN Potomac River INVESTIGATORS MDahl JPangle SRobertson W Bousqet BMillholland JTovar H Kepple
DATE 30 May 2008 TIME 1050 AM (start)
PROJECT NAME
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) YES x NO
rain (steady rain) Air Temperature __18___degC showers (intermittent) Comments _______________________
_85_x cloud cover _10__ ______________________________
clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x (Significant springs andor seeps) x ( Valley) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS Multi-habitat sampling with D-frame nets and seine
m
n
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial x No evidence Some evidence x FieldPasture Industrial Obvious sources x Agricultural Other ____________ Minor stream bank scour Residential Local Watershed Erosion x None Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left 35 bank grasses scattered trees and some bushes Right High bank with grasses and scattered trees Indicate the dominant vegetation type(s) and record the dominant species
x Trees Shrubs Grasses Forbs Dominant species Black Walnut and Festuca elatior
INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width __2___m Fully open Partly open x Partly shaded Fully shaded Sampling reach area __200_m2
Estimated stream depth __03_m Proportion of Reach Represented by Stream High water mark __1___m Morphology Types Surface velocity (at thalweg) __166_msec x Riffle__0___ x Run__55___ x Pool__45___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___2____ m2 Density of LWD ___-____ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __20_____ x Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present ____Glyceria (grass)sp__________
WATER QUALITY Temperature __16___degC Water Odors x NormalNone Sewage Specific Conductance ___-___ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ____-____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used ______NA___ Clear x Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other__NONE__ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 0 Detritus sticks wood coarse plant materials
5 Boulder gt256 mm (10rsquorsquo) 0
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 27 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 38 Sand 006-2 mm (gritty) 5 Marl grey shell
fragments 0 (lt1)
Silt 0004-006 mm 25 Clay lt 0004 mm (slick) 5 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Appendix B
WATER QUALITY ANALYSIS THROUGH THE VIRGINIA
STREAM CONDITION INDEX (VSCI)
The Virginia Stream Condition Index (VSCI) relies on eight metrics to categorize stream health According to Burton and Gerritsen (2003 p 1-1) this index ldquohellip can be used as a primary indicator of ecosystem health and can identify impairment with respect to the reference (or natural) conditionrdquo These metrics are numeric measures of a biological communityrsquos species composition diversity ecological niches or tolerance to pollution
Table 13 on the next page describes each VSCI metric lists its formula and provides the best standard value (BSV) for that metric Based on a 100-point scale the BSV is the score that reflects optimum conditions for aquatic life ie the best water quality value possible (DEQ 2006b pp 8 33) The table also explains whether the score increases or decreases with environmental stress
As Table 13 indicates each of the eight VSCI metrics is calculated individually Each formula provides a unitless score based on a 100-point scale with 0 as the worst possible score and 100 as the best Any metric that provides a result above 100 is changed to 100 in order for the index to work properly After the eight metrics are calculated they are summed and averaged to provide a final VSCI score on a 100-point scale
Based on statistical analysis of Virginiarsquos streams and their BMI communities the DEQ (2006b p 8) currently uses the following tiers to interpret VSCI scores
VSCI Scores Aquatic Life Use
(ALI) Tiers
lt 42 severely stressed
42-55 moderately stressed
55-63 unknownfair (gray zone)
63-73 good
gt73 excellent
Section 2 of this report presents the results of calculations for the six BMI sampling sites the research team studied in the Hiatt-Lick Run subwatershed in 2008
o
p
1 Total taxa the number of distinct taxa in the sample measures the overall diversity of the macroinvertebrate assemblage The best standard value (BSV) is 189 Score = 100 x (X189) Total taxa decreases with stress 2 EPT taxa the sum of the distinct taxa in the orders Ephemeroptera (mayflies) Plecoptera (stoneflies) and Trichoptera (caddisflies) These orders are known to be sensitive to pollution The best standard value (BSV) is 117 Score = 100 x (X117) EPT taxa decreases with stress 3 Percent Ephemeroptera the percent abundance of individuals in the sample that belong to the order Ephemeroptera (mayflies) The best standard value (BSV) is 477 Score = 100 x (X477) Percent Ephemeroptera decreases with stress 4 Percent Plecoptera + Trichoptera less Hydropsychidae the percent abundance of individuals in the sample that belong to the orders Plecoptera (stoneflies) and Trichoptera (caddisflies) excluding the pollution-tolerant caddisfly family Hydropsychidae The best standard value (BSV) is 562 Score = 100 x (X562) Percent Plecoptera + Trichoptera less Hydropsychidae decreases with stress 5 Percent Scrapers the percent abundance of individuals in the sample whose primary functional mechanism for feeding is grazing on the substrate or periphyton (ie attached algae and associated material) The best standard value (BSV) is 467 Score = 100 x (X467) Percent scrapers decreases with stress 6 Percent Chironomidae the percent abundance of individuals in the sample that are Chironomidae (non-biting midge) larvae and pupae The best standard value (BSV) is 16 Score = 100 x [(100-X)(100-16)] Percent Chironomidae increases with stress 7 Percent Top Two Dominant the percent abundance of the two most frequent taxa present in the sample The best standard value (BSV) is 328 Score = 100 x [(100-X)(100-328)] The Percent Top 2 Dominant increases with stress 8 Hilsenhoff Biotic Index (HBI) an abundance-weighted average pollution tolerance of all the individuals within a sample keyed to the family level Tolerance ratings range from 1 (highly intolerant) to 10 (highly tolerant) The best standard value (BSV) is 29 Score = 100 x [(10-X)(10-29)] The Hilsenhoff Biotic Index (HBI) increases with stress ______________________________________________________________________________ Table 13 Description of Benthic Macroinvertebrate VSCI Metrics Includes responses (increases decreases) of each VSCI metric to degraded water quality conditions Adapted from Burton and Gerritsen (2003 pp 3-15 3-16 6-2) and DEQ (2008)
q
Appendix C
LABORATORY BENCH SHEETS
Shenandoah Universityrsquos Environmental Studies Department developed the Benthic Macroinvertebrate Laboratory Bench Sheet and VSCI Worksheet to record BMI specimen identifications and their VSCI classifications for each sample
This form is based in part on bench sheets developed by the EPA and Virginia DEQ for recording specimen identifications Additional columns on Shenandoah Universityrsquos forms for relative numbers and VSCI classifications make the calculation and verification of VSCI scores easier
Note the column for taxonomistrsquos initials Specimens were identified by students in Shenandoah Universityrsquos research group and then verified or where necessary changed by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality This external review helps assure the validity of the study Originals of these bench sheets with handwritten taxonomistsrsquo initials will be kept on file at Shenandoah Universityrsquos Environmental Studies Department for five years after the completion of this study
r
s
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-1 STATION NAME Mouth LAT N 39deg 12749rsquo (upstream) LONG W 078deg 04209rsquo (upstream) LAT N 39deg 12729rsquo (downstream) LONG W 078deg 04148rsquo (downstream) FORM COMPILED BY Jeremy Tovar W S Bousquet RIVER BASIN Potomac River INVESTIGATORS Marie Dahl Brandon Millholand J W Pangle Sean Robertson Jeremy Tovar Woodward Bousquet Ben Bousquet
Collection date 21 May 2008 Sheet completion date 12 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Hirudinea 3 33 JT BVW PR 8 SP Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Coenagrionidae 2 22 BVW PR 9 CR Ephemeroptera (mayflies) Caenidae 1 11 JT BVW CG 6 SP Ephemerllidae 4 44 JT BVW CG 2 CR Heptageniidae 5 55 JT BVW SC 3 CG Isonychiidae 1 11 BVW CF 3 CG Plecoptera (stoneflies) Perlidae 14 154 JT BVW PR 2 CR Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Corydalidae 1 11 JT BVW PR 5 CR Coleoptera (beetles) Elmidae 24 264 I JT BVW SC 4 CG Dytiscidae 4 44 JT BVW PR 6 GN Psephenidae 3 33 JT BVW SC 4 CG Diptera (true flies) Tipulidae 3 33 JT BVW SH 4 BU Chironomidae 26 286 JT BVW CG 6 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
t
Other Total Individuals 91 1001 Total Taxa 13
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
u
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-1 STATION NAME Mouth LAT N 39deg 12749rsquo (upstream) LONG W 078deg 04209rsquo (upstream) LAT N 39deg 12729rsquo (downstream) LONG W 078deg 04148rsquo (downstream) FORM COMPILED BY Jeremy Tovar W S Bousquet RIVER BASIN Potomac River INVESTIGATORS Brandon Millholand JW Pangle Jeremy Tovar Heather Kepple
Collection date 10 October 2008 Sheet completion date 12 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Oligochaeta 3 35 JT BVW CG 8 BU Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Coenagrionidae 2 23 JT BVW PR 9 CR Ephemeroptera (mayflies) Heptageniidae 12 140 JT BVW SC 3 CG Isonychiidae 2 23 BVW CF 3 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Hydropsychidae 16 186 JT BVW CF 6 CG Philopotamidae 31 360 JT BVW CF 2 CG Hemiptera (true bugs) (Veliidae) (5) - JT BVW ( ) Megaloptera (dobsonflies etc) Corydalidae 10 116 JT BVW PR 5 CR Coleoptera (beetles) Dytiscidae 1 12 JT BVW PR 6 GN Psephenidae 7 81 JT BVW SC 4 CG Diptera (true flies) Tipulidae 2 23 BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
v
Other Total Individuals 86
(91) 999
Total Taxa 10 (11)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009 Veliidae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
w
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-2 STATION NAME Package Plant LAT N 39deg 12958rsquo (upstream) LONG W 078deg 04900 (upstream) LAT N 39deg 12958rsquo (downstream) LONG W 078deg 04822 (downstream) FORM COMPILED BY JW Pangle W Bousquet RIVER BASIN Potomac River INVESTIGATORS JW Pangle S Robertson J Tovar M Dahl B Millholland W Bousquet
Collection date 29 May 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Plecoptera (stoneflies) Perlidae 11 136 I JP BVW PR 1 CR Trichoptera (caddisflies) Hydropsychidae 10 123 I JP BVW CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 14 173 I JP BVW SC 4 CG Diptera (true flies) Chironomidae 32 395 I JP BVW CG 6 BU Simuliidae 14 173 I JP BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
x
Other Total Individuals 81 1000 Total Taxa 5
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-2 STATION NAME Package Plant LAT N 39deg 12958rsquo (upstream) LONG W 078deg 04900 (upstream) LAT N 39deg 12958rsquo (downstream) LONG W 078deg 04822 (downstream) FORM COMPILED BY JW Pangle W Bousquet RIVER BASIN Potomac River INVESTIGATORS JW Pangle Brandon Millholland Jeremy Tovar Heather Kepple
Collection date 10 October 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Heptageniidae 40 392 I WBJP SC 3 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Hydropsychidae 7 69 I JP WB BVW CF 6 CG Philopotamidae 28 275 I JP WB BVW CF 2 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Corydalidae 6 59 I JP WB BVW PR 5 CR Coleoptera (beetles) Psephenidae 7 69 I JP WB BVW SC 4 CG Elmidae 8 78 I JP WB BVW SC 4 CG Diptera (true flies) Tipulidae 6 59 I JP WB BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
y
z
Other Total Individuals 102 1001 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-3 STATION NAME Rt 664 Bridge LAT N 39deg 12934rsquo (upstream) LONG W 078deg 05019rsquo (upstream) LAT N 39deg 12951rsquo (downstream) LONG W 078deg 04958rsquo (downstream) FORM COMPILED BY S Robertson W Bousquet RIVER BASIN Potomac River INVESTIGATORS SRobertson BMillholland WBousquet MDahl JPangle
Collection date 28 May 2008 Sheet completion date 11 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Leptophlebiidae 1 15 I SGR BVW CG 2 CR Baetidae 1 15 I BVW CG 5 CG Plecoptera (stoneflies) Perlidae 3 46 I SGR BVW PR 1 CR Nemouridae 1 15 I BVW SH 3 CR Trichoptera (caddisflies) Hydropsychidae 5 77 I SGR BVW CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 27 415 I SGR BVW SC 4 CG Psephenidae 2 31 A SGR BVW SC 4 CG Diptera (true flies) Simuliidae 5 77 I SGR BVW CF 6 CG Chironomidae 20 308 I SGR BVW CG 6 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
aa
bb
Other Total Individuals 65 999 Total Taxa 9
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-3 STATION NAME Rt 664 Bridge LAT N 39deg 12934rsquo (upstream) LONG W 078deg 05019rsquo (upstream) LAT N 39deg 12951rsquo (downstream) LONG W 078deg 04958rsquo (downstream) FORM COMPILED BY S Robertson RIVER BASIN Potomac River INVESTIGATORS S Robertson M Dahl A Fisher H Kepple
Collection date 10 Oct 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Heptageniidae 2 20 I SGR SC 3 CG Isonychiidae 2 20 I BVW CF 2 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Philopotamidae 36 367 I SGR CF 2 CG Hydropsychidae 47 480 I SGR CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 2 20 A SGR SC 4 CG Psephenidae 8 82 A SGR SC 4 CG Diptera (true flies) Tabanidae 1 10 I SGR PR 7 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
cc
dd
Other Total Individuals 98 999 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-4 STATION NAME Unnamed Tributary LAT (upstream) N 39deg 13035rsquo LONG (upstream) W 078deg 05129rsquo LAT (downstream) N 39deg 13000rsquo LONG (downstream) W 078deg 5086rsquo FORM COMPILED BY A Fisher M Dahl rev W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson A Fisher H Kepple
Collection date 31 May 2008 Sheet completion date rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Flatworms (Turbellaria) Worms (Oligochaeta) Naididae 1 14 - BVW CG 8 BU Leeches (Hirudinea) Snails (Gastropoda) Clams (Bivalvia) Isopods (Isopoda) Asellidae 3 42 A AF BVW CG 8 SP Scuds (Amphipoda) Crayfish Shrimp (Decapoda) Water Mites (Hydracarina) Dragonflies (Odonata Anisoptera) Damselflies (Odonata Zygoptera) Mayflies (Ephemeroptera) Stoneflies (Plecoptera) Perlidae 2 28 I AF BVW PR 1 CR Caddisflies (Trichoptera) True Bugs (Hemiptera) Corixidae 1 14 I AF BVW MP 9 DV Dobsonflies (Megaloptera) Beetles (Coleoptera) Dytiscidae 1 14 I AF BVW PR 6 GN True Flies (Diptera) Chironomidae 61 847 I AF BVW CG 6 BU Simuliidae 3 42 I AF BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
ee
ff
Other Total Individuals 72 1001 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-4 STATION NAME Unnamed Tributary LAT (upstream) N 39deg 13035rsquo LONG (upstream) W 078deg 05129rsquo LAT (downstream) N 39deg 13000rsquo LONG (downstream) W 078deg 5086rsquo FORM COMPILED BY Andrew G M Fisher W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson A Fisher H Kepple
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Flatworms (Turbellaria) Worms (Oligochaeta) Lumbriculidae 2 32 A AF BVW CG 8 BU Leeches (Hirudinea) Snails (Gastropoda) Lymnaeidae 1 16 A BVW CG 9 GN Clams (Bivalvia) Isopods (Isopoda) Scuds (Amphipoda) Crayfish Shrimp (Decapoda) Cambaridae 1 16 A AF BVW GN 5 GN Water Mites (Hydracarina) Dragonflies (Odonata Anisoptera) Damselflies (Odonata Zygoptera) Calopterygidae 4 63 I BVW PR 7 CL Mayflies (Ephemeroptera) Tricorythidae 1 16 I BVW CG 5 SP Stoneflies (Plecoptera) Caddisflies (Trichoptera) Phryganeidae 4 63 I AF BVW SH 4 CR Hydropsychidae 6 95 I AF BVW CF 6 CG I True Bugs (Hemiptera) Corixidae 8 127 I AF BVW MP 9 DV (Veliidae ) (7) - I AF BVW ( ) Dobsonflies (Megaloptera) Sialidae 3 48 I AF BVW PR 7 SP Corydalidae 2 32 I AF BVW PR 5 CR Beetles (Coleoptera) Haliplidae 1 16 A AF BVW MP 8 GN Dysticidae 7 111 I AF BVW PR 6 GN Hydrophilidae 1 16 A AF BVW PR 8 GN Elmidae 3 48 I BVW SC 4 CG True Flies (Diptera) Chironomidae 8 127 I AF BVW CG 6 BU Simuliidae 7 111 I AF BVW CF 6 CG Tipulidae 4 63 I AF BVW SH 4 GU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
gg
hh
Other Total Individuals 63
(70) 1000
Total Taxa 17 (18)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Veliidae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-5 STATION NAME Lick Run LAT N 39deg 12752rsquo (upstream) LONG W 078deg 05685rsquo (upstream) LAT N 39deg 12784rsquo (downstream) LONG W 078deg 05631rsquo (downstream) FORM COMPILED BY Brandon Millholland W Bousquet RIVER BASIN Potomac River INVESTIGATORS J Pangle S Robertson W Bousquet JTovar M Dahl
Collection date 29 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Gomphidae 1 15 I BVW PR 3 BU Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Leptophlebiidae 11 167 A LBM BVW CG 2 CR Plecoptera (stoneflies) Perlidae 10 152 A LBM BVW PR 1 CR Perlodiae 1 15 A LBM BVW PR 2 CR Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 5 76 A LBM BVW SC 4 CG Elmidae 2 30 I LBM BVW SC 4 CG Diptera (true flies) (Culicidae) (3) - P LMB BVW ( ) Chironomidae 23 348 I LBM BVW CG 6 BU Simuliidae 13 197 I LBM BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
ii
jj
Other Total Individuals 66
(69) 1000
Total Taxa 7 (8)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Culicidae (Diptera) pupae not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl J Pangle S Robertson W Bousquet B Millholland J Tovar H Kepple
Collection date 30 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 1 12 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Asellidae 114 965 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Plecoptera (stoneflies) Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 2 23 A MRD BVW SC 4 CG Diptera (true flies)
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
kk
ll
Other Total Individuals 117 1000 Total Taxa 3
Notes Subsample taken 29 October 2009 All taxonomic determinations by MRD were confirmed by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson K McCann A Fisher H Kepple A Barrett
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 2 17 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Sphaeriidae 2 17 I MRD BVW CF 8 BU Isopoda (isopods) Asellidae 99 853 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Calopterygidae 2 17 A MRD BVW PR 7 CL Ephemeroptera (mayflies) Leptophlebiidae 2 17 I MRD BVW CG 2 CR Heptageniidae 4 34 A MRD BVW SC 3 CG Plecoptera (stoneflies) Perlidae 1 09 A MRD BVW PR 1 CR Trichoptera (caddisflies) Hemiptera (true bugs) (Gerridae) (1) - A MRD BVW ( ) Corixidae 1 09 A MRD BVW PR 5 CR Megaloptera (dobsonflies etc) Coleoptera (beetles) Dryopidae 1 09 A MRD BVW SC 5 CG Diptera (true flies) Tipulidae 2 17 I BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
mm
nn
Other Total Individuals 116
(117) 999
Total Taxa 10 (11)
Notes Subsample taken 6 November 2008 Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009 Gerridae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
- Disclaimer ii
- Title Page iii
- Acknowledgements v
- Table of Contents vii
- SECTION 2 RESULTS DATA TABLES 7
- OVERVIEW AND FINDINGS
- Introduction
- RESULTS DATA TABLES
- REFERENCES
- FIELD DATA SHEETS
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- SubstrateType
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- WATER QUALITY ANALYSIS
- THROUGH THE VIRGINIA
- STREAM CONDITION INDEX (VSCI)
- LABORATORY BENCH SHEETS
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
Site HLR-6 McCann Farm
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 3 189 1592 EPT taxa 0 117 003 Ephemeroptera 00 477 004 Plec +Tric less Hydropsych 00 562 005 Scrapers 23 467 496 Chironomidae 00 16 10007 Top 2 Dominant 988 328 188 Hilsenhoff Biotic Index (HBI) 79 29 296VSCI Score 190
According to the DEQrsquos water quality standards aquatic life at this site is severely stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 11 VSCI Scores for McCann Farm (HLR-6) Site in Hiatt-Lick Run Sampled 30 May 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
VSCI Metric
Number or Percent
Best Standard Value (BSV)
Score
1 Taxa Richness 10 189 5292 EPT taxa 3 117 2563 Ephemeroptera 51 477 1074 Plec +Tric less Hydropsych 09 562 165 Scrapers 43 467 926 Chironomidae 00 16 10007 Top 2 Dominant 887 328 1688 Hilsenhoff Biotic Index (HBI) 75 29 352VSCI Score 315
According to the DEQrsquos water quality standards aquatic life at this site is severely stressed See Burton and Gerritsen (2003) and DEQ (2006b 2008) Table 12 VSCI Scores for McCann Farm (HLR-6) Site in Hiatt-Lick Run Sampled 10 October 2008
Prepared by Environmental Studies Department Shenandoah University Winchester VA 2009
12
REFERENCES
Barbour Michael T Jeroen Gerritsen Blaine D Snyder and James B Stribling 1999 Rapid bioassessment protocols for use in streams and wadeable rivers periphyton benthic macroinvertebrates and fish Second edition EPA 841-B-99-002 Washington DC US Environmental Protection Agency Office of Water Available online at httpwwwepagovOWOWmonitoringbioasspshtml Burton June and Jeroen Gerritsen 2003 A stream condition index for Virginia non-coastal streams Prepared for the US EPA Office of Science and Technology US EPA Region 3 Environmental Services Division and Virginia Department of Environmental Quality Owings Mills Maryland Tetra Tech Inc Available online at httpwwwdeqvirginiagovwatermonitoringpdf vastrmconpdf Hessler Rachel A Andrea R Martin Heidi D Ruggeri William B Seymour Jr and Woodward S Bousquet 2006 Water quality assessment using benthic macroinvertebrates A study and compilation Winchester Virginia Environmental Studies Program Shenandoah University Occasional publication no 10 Igoe Robert 2006 DEQ identifies nine impaired streams in Clarke Frederick The Winchester Star 15 July B1 Jackson Charlie 2005 Survey targets Opequon cleanup The Winchester Star 19 August B1 Johnston Elizabeth A and Woodward S Bousquet 2003 What happens when it rains A study of water quality in Abrams Creek and Town Run Winchester and Frederick County Virginia Winchester Virginia Environmental Studies Program Shenandoah University Occasional publication no7 Merritt Richard W and Kenneth W Cummins 1996 An introduction to the aquatic insects of North America Dubuque Iowa KendallHunt Publishing Company Opequon Creek IP Steering Committee Virginia Tech Department of Biological Systems Engineering and Center for TMDL and Watershed Studies University of Virginia Institute for Environmental Negotiation and West Virginia University Division of Resource Management 2006 Opequon Creek watershed TMDL implementation plan Blacksburg Virginia Virginia Tech Department of Biological Systems Engineering Available online at httpwwwtmdlbsevteduoutreachC82 Pennak Robert W 1953 Fresh-water invertebrates of the United States New York New York The Ronald Press Company Rosenburg David M and Vincent H Resh 1993 Freshwater biomonitoring and benthic macroinvertebrates New York New York Chapman amp Hall Shenandoah University Public Relations Office 2002 Data collected by SU and its partners helps identify impaired water Winchester Virginia Available online at httpwwwsuedutemp_ newscfmurlnum=32 Thorp James H and Alan P Covich 1991 Ecology and classification of North American freshwater invertebrates San Diego California Academic Press Inc
13
US Environmental Protection Agency 1997 Volunteer stream monitoring a methods manual EPA 841-B-97-003 Washington DC US Environmental Protection Agency Office of Water Van Meter Val 2003 Opequon Creek pollution limits to be established The Winchester Star 4 April B1 Virginia Department of Environmental Quality 2006a Virginia 305(b)303(d) water quality integrated report to Congress and the EPA Administrator for the period January 1 2000 to December 31 2004 Richmond Virginia Virginia Department of Environmental Quality Available online at httpwwwdeqvirginiagovwqair2006html __________ 2006b Using probabilistic data to validate the non-coastal Virginia Stream Condition Index (draft) VDEQ technical bulletin WQA2006-001 Virginia Department of Environmental Quality Available online at httpwwwloudounwatershedorgstream_assessmentreference_documents VA_DEQ_SCI_Validationpdf __________ 2008 Biological monitoring program quality assurance project plan for wadeable streams and rivers Richmond Virginia Virginia Department of Environmental Quality Update available online at httpwwwdeqstatevausbiomonitoringdocumentsBioMonQAPP_13Aug2008pdf Virginia Tech Aquatic Entomology Laboratory 2005 Taxa dictionary [Excel file] Blacksburg Virginia Virginia Tech Department of Biological Systems Engineering 2003a Opequon watershed TMDLs for benthic impairments Abrams Creek and Lower Opequon Creek Frederick and Clarke Counties Virginia Prepared for the Virginia Department of Environmental Quality and Virginia Department of Conservation and Recreation Blacksburg Virginia Available online at httpwwwdeqstate vaustmdlapptmdlsshenrvrabropebcpdf Voshell J Reese Jr 2002 A guide to common freshwater invertebrates of North America Blacksburg Virginia The McDonald amp Woodward Publishing Company
14
APPENDICES
15
16
a
Appendix A
FIELD DATA SHEETS
This appendix presents a Site Characterization Field Data Sheet for each of the six sampling reaches established on Hiatt Run and Lick Run for this study They were filled out during the sampling runs made in May 2008
These forms were adapted by Shenandoah University from forms used by the EPA and Virginia DEQ for recording characteristics of sampling sites
b
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Mouth STATION _HLR-1_____ STREAM CLASS LAT N 39 12749 (upstream) LONG W 078 04209(upstream) LAT N 39 12729 (downstream) LONG W 078 04148 (downstream) FORM COMPILED BY Jeremy Tovar RIVER BASIN Potomac River INVESTIGATORS JPangle B Millholland MDahl SRobertson W Bousquet JTovar BBousquet
DATE May 21 2008_________ TIME_250 PM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature __24___degC showers (intermittent) Comments _______________________
___ cloud cover _0_ ______________________________
X clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x ( Significant springs andor seeps) x (Valley ) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS
c
d
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence x FieldPasture Industrial Obvious sources Agricultural Other ____________ Stream scour steep slope erosion x Residential Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left Field and young thin deciduous forest 100 Bank=Grass cobble and shrub Right 90 maturing deciduous forest 45 shale slope Bank=Bedrock soil and herbs Indicate the dominant vegetation type(s) and record the dominant species
x Trees Shrubs x (Grasses) Forbs Dominant species Oak species and mix deciduous
INSTREAM FEATURES
Measured reach length ___100__m Canopy Cover Estimated stream width _35__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area __350_m2
Estimated stream depth _008_m Proportion of Reach Represented by Stream High water mark _15_m Morphology Types Surface velocity (at thalweg) _05__msec x Riffle__75___ x Run__20___ x Pool__5___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___00__ m2 Density of LWD _-_ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __15_____ x Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present Algae
WATER QUALITY Temperature __19___degC Water Odors x NormalNone Sewage Specific Conductance - Petroleum Chemical Fishy Other___________________ Dissolved Oxygen - Water Surface Oils Slick Sheen Globs Flecks pH __-_ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used _____NA________ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic x None Relict shells Other______None_____ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 8 Detritus sticks wood coarse plant materials
0 Boulder gt256 mm (10rsquorsquo) 0
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 51 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 40 Sand 006-2 mm (gritty) 1 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University
SITE CHARACTERIZATION FIELD DATA SHEET FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Package Plant STATION HLR-2 STREAM CLASS LAT N 39 129580 (upstream) LONG W 078 04900 (upstream) LAT N 39 129581 (downstream) LONG W 078 04822 (downstream) FORM COMPILED BY JW Pangle RIVER BASIN Potomac River INVESTIGATORS JPangle JTovar MDahl B Millholland SRobertson W Bousquet
DATE__19 May 2008_ TIME__1240__ PM
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature _____degC showers (intermittent) Comments __Sunny Day_____________
_5__ cloud cover _0__ ______________________________
x clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x (Significant springs andor seeps) x (Valley) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS
e
f
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence FieldPasture Industrial Obvious sources Agricultural Other ____________ __Upstream Construction and Stream bank Scour Residential __________________________________ Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left Deciduous forest with thick herb and shrub layers bank= vegetation and rocks Right Deciduous forest with thick herb and shrub layers bank=vegetation soil and rocks Indicate the dominant vegetation type(s) and record the dominant species
x Trees x Shrubs Grasses Forbs Dominant species Black Walnut White Ash Hackberry and American Sycamore
INSTREAM FEATURES
Measured reach length 100 m Canopy Cover Estimated stream width _35__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area __350_m2
Estimated stream depth __15__m Proportion of Reach Represented by Stream High water mark __22___m Morphology Types Surface velocity (at thalweg) _035__msec x Riffle__30___ x Run__30___ x Pool__40___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___10____ m2 Density of LWD _______ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __30___ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present__________________________________________________________
WATER QUALITY Temperature __18___degC Water Odors x NormalNone Sewage Specific Conductance __-____ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ____-____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used _____NA____ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other___None_____ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach SubstrateType Characteristic
Composition in Sampling Reach
Bedrock 3 Detritus sticks wood coarse plant materials
2 Boulder gt256 mm (10rsquorsquo) 5
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 70 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 22 Sand 006-2 mm (gritty) 0 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiattrsquos Run LOCATION Rt 664 Bridge STATION HLR-3 STREAM CLASS LAT N 39 12934 (upstream) LONG W 078 05019 (upstream) LAT N 39 12958 (downstream) LONG W 078 04958 (downstream) FORM COMPILED BY Sean Robertson RIVER BASIN Potomac River INVESTIGATORS SRobertson BMillholland WBousquet MDahl JPangle
DATE 28 May 2008 TIME 1127 AM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality 2008
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature __14___degC showers (intermittent) x Trace Comments _______________________
_65 x cloud cover ___ ______________________________
x clearsunny __________________________________
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x (Significant springs andor seeps) x (Valley) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS
g
h
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence Some evidence x FieldPasture Industrial x Obvious sources Agricultural x Other Old Package Plant Downstream x Residential Bridge and Road Power line and Pump station Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left 70 field 30 shrubs and deciduous trees Bank=Soil and vegetation Right Maturing deciduous forest steep slope Bank=Shale Bedrock Indicate the dominant vegetation type(s) and record the dominant species
x Trees x Shrubs x Grasses Forbs Dominant species Sycamore and Red Oaks
INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width __51___m Fully open x Partly open Partly shaded Fully shaded Sampling reach area _510_m2
Estimated stream depth __135___m Proportion of Reach Represented by Stream High water mark __-___m Morphology Types Surface velocity (at thalweg) __20___msec x Riffle_40_ x Run_50_ x Pool 10 Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___0____ m2 Density of LWD ___-____ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation ___50____ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present Brown Algae
WATER QUALITY Temperature __17___degC Water Odors x NormalNone Sewage Specific Conductance ___-___ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ___-_____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used ____NA_______ Clear x Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other____None____ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 15 Detritus sticks wood coarse plant materials
0 Boulder gt256 mm (10rsquorsquo) 15
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 62 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 8 Sand 006-2 mm (gritty) 0 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Unnamed Tributary STATION HLR-4 STREAM CLASS LAT N 39 13035 (upstream) LONG W 078 05129 (upstream) LAT N 39 13000 (downstream) LONG W 078 05086 (downstream) FORM COMPILED BY Marie Dahl RIVER BASIN Potomac River INVESTIGATORS M Dahl HKepple SRobertson AFisher
DATE 31 May 2008 TIME 5 PM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality 2008
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) YES x NO
rain (steady rain) Air Temperature __22___degC showers (intermittent) Comments _______________________
_0__ cloud cover _0__ ______________________________
x clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area __-___ km2 mi2 Montane Significant springs andor seeps x Valley Marsh or swamp Montane amp Valley Mixture of origins Other ___________________
COMMENTS Snowden Bridge development (upstream) and sewer line
i
j
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence x FieldPasture Industrial Obvious sources Agricultural Other ____________ Construction upstream and adjacent x Residential Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left Forested with rocky banks field across silt fence and 2m tree strip Right Forested with some rocky banks and forest through the highway Indicate the dominant vegetation type(s) and record the dominant species
x Trees x Shrubs x Grasses Forbs Dominant species White Ash and Hackberry
INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width ___38__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area __380__m2
Estimated stream depth __12__cm Proportion of Reach Represented by Stream High water mark __2___m Morphology Types Surface velocity (at thalweg) __46___msec x Riffle__25___ x Run__70___ x Pool__5___ Channelized Yes x No Dam Present x Yes No
LARGE WOODY DEBRIS
LWD _______ m2 Density of LWD _______ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation ___NA____ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present___Algae___
WATER QUALITY Temperature __16___degC Water Odors x NormalNone Sewage Specific Conductance __-____ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ___-_____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used ____NA_____ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells x Other___Shale_______ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 0 Detritus sticks wood coarse plant materials
10 Boulder gt256 mm (10rsquorsquo) 5
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 45 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 30 Sand 006-2 mm (gritty) 0 Marl grey shell
fragments 0
Silt 0004-006 mm 20 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Lick Run STATION HLR-5 STREAM CLASS LAT N 39 127517 (upstream) LONG W 078 05685(upstream) LAT N 39 12784 (downstream) LONG W 078 05631 (downstream) FORM COMPILED BY B Millholland RIVER BASIN Potomac River INVESTIGATORS B Millholland JTovar SRobertson MDahl WBousquet JPangle
DATE 29 May 2008 TIME 1040 AM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project
k
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature __15___degC showers (intermittent) Comments _______________________
_5_ X cloud cover _0__ ______________________________
x clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane Significant springs andor seeps x Valley Marsh or swamp Montane amp Valley Mixture of origins Other ___________________
COMMENTS Lick Run appears to be intermittent and may be dry in Fall 2008
l
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence x FieldPasture Industrial Obvious sources Agricultural Other ____________ Adjacent logging stream bank scour Residential Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left 85 young deciduous forest 15 logged Bank=Soil vegetation and cobbles Right Young deciduous forest on 30 slope Bank= Soil vegetation and cobble Indicate the dominant vegetation type(s) and record the dominant species
x Trees Shrubs Grasses Forbs Dominant species Mixed Deciduous INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width __11__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area 110 m2
Estimated stream depth _009__m Proportion of Reach Represented by Stream High water mark __96___m Morphology Types Surface velocity (at thalweg) __01___msec x Riffle__30___ x Run__5___ x Pool__65___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___0____ m2 Density of LWD ___-____ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __70___ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present__________________________________________________________
WATER QUALITY Temperature __14___degC Water Odors x NormalNone Sewage Specific Conductance __-__ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ___-___ Water Surface Oils Slick Sheen Globs Flecks pH __-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used _____NA______ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other_____None_____
SEDIMENT AND SUBSTRATE
Odors Deposits Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other________________ x Other Slightly Salty Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 0 Detritus sticks wood coarse plant materials
5 Boulder gt256 mm (10rsquorsquo) 0
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 75 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 23 Sand 006-2 mm (gritty) 2 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION McCann Farm STATION HLR-6 STREAM CLASS LAT N 39 13076 (upstream) LONG W 078 06539 (upstream) LAT N 39 13063 (downstream) LONG W 078 06473 (downstream) FORM COMPILED BY MDahl RIVER BASIN Potomac River INVESTIGATORS MDahl JPangle SRobertson W Bousqet BMillholland JTovar H Kepple
DATE 30 May 2008 TIME 1050 AM (start)
PROJECT NAME
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) YES x NO
rain (steady rain) Air Temperature __18___degC showers (intermittent) Comments _______________________
_85_x cloud cover _10__ ______________________________
clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x (Significant springs andor seeps) x ( Valley) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS Multi-habitat sampling with D-frame nets and seine
m
n
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial x No evidence Some evidence x FieldPasture Industrial Obvious sources x Agricultural Other ____________ Minor stream bank scour Residential Local Watershed Erosion x None Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left 35 bank grasses scattered trees and some bushes Right High bank with grasses and scattered trees Indicate the dominant vegetation type(s) and record the dominant species
x Trees Shrubs Grasses Forbs Dominant species Black Walnut and Festuca elatior
INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width __2___m Fully open Partly open x Partly shaded Fully shaded Sampling reach area __200_m2
Estimated stream depth __03_m Proportion of Reach Represented by Stream High water mark __1___m Morphology Types Surface velocity (at thalweg) __166_msec x Riffle__0___ x Run__55___ x Pool__45___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___2____ m2 Density of LWD ___-____ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __20_____ x Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present ____Glyceria (grass)sp__________
WATER QUALITY Temperature __16___degC Water Odors x NormalNone Sewage Specific Conductance ___-___ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ____-____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used ______NA___ Clear x Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other__NONE__ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 0 Detritus sticks wood coarse plant materials
5 Boulder gt256 mm (10rsquorsquo) 0
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 27 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 38 Sand 006-2 mm (gritty) 5 Marl grey shell
fragments 0 (lt1)
Silt 0004-006 mm 25 Clay lt 0004 mm (slick) 5 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Appendix B
WATER QUALITY ANALYSIS THROUGH THE VIRGINIA
STREAM CONDITION INDEX (VSCI)
The Virginia Stream Condition Index (VSCI) relies on eight metrics to categorize stream health According to Burton and Gerritsen (2003 p 1-1) this index ldquohellip can be used as a primary indicator of ecosystem health and can identify impairment with respect to the reference (or natural) conditionrdquo These metrics are numeric measures of a biological communityrsquos species composition diversity ecological niches or tolerance to pollution
Table 13 on the next page describes each VSCI metric lists its formula and provides the best standard value (BSV) for that metric Based on a 100-point scale the BSV is the score that reflects optimum conditions for aquatic life ie the best water quality value possible (DEQ 2006b pp 8 33) The table also explains whether the score increases or decreases with environmental stress
As Table 13 indicates each of the eight VSCI metrics is calculated individually Each formula provides a unitless score based on a 100-point scale with 0 as the worst possible score and 100 as the best Any metric that provides a result above 100 is changed to 100 in order for the index to work properly After the eight metrics are calculated they are summed and averaged to provide a final VSCI score on a 100-point scale
Based on statistical analysis of Virginiarsquos streams and their BMI communities the DEQ (2006b p 8) currently uses the following tiers to interpret VSCI scores
VSCI Scores Aquatic Life Use
(ALI) Tiers
lt 42 severely stressed
42-55 moderately stressed
55-63 unknownfair (gray zone)
63-73 good
gt73 excellent
Section 2 of this report presents the results of calculations for the six BMI sampling sites the research team studied in the Hiatt-Lick Run subwatershed in 2008
o
p
1 Total taxa the number of distinct taxa in the sample measures the overall diversity of the macroinvertebrate assemblage The best standard value (BSV) is 189 Score = 100 x (X189) Total taxa decreases with stress 2 EPT taxa the sum of the distinct taxa in the orders Ephemeroptera (mayflies) Plecoptera (stoneflies) and Trichoptera (caddisflies) These orders are known to be sensitive to pollution The best standard value (BSV) is 117 Score = 100 x (X117) EPT taxa decreases with stress 3 Percent Ephemeroptera the percent abundance of individuals in the sample that belong to the order Ephemeroptera (mayflies) The best standard value (BSV) is 477 Score = 100 x (X477) Percent Ephemeroptera decreases with stress 4 Percent Plecoptera + Trichoptera less Hydropsychidae the percent abundance of individuals in the sample that belong to the orders Plecoptera (stoneflies) and Trichoptera (caddisflies) excluding the pollution-tolerant caddisfly family Hydropsychidae The best standard value (BSV) is 562 Score = 100 x (X562) Percent Plecoptera + Trichoptera less Hydropsychidae decreases with stress 5 Percent Scrapers the percent abundance of individuals in the sample whose primary functional mechanism for feeding is grazing on the substrate or periphyton (ie attached algae and associated material) The best standard value (BSV) is 467 Score = 100 x (X467) Percent scrapers decreases with stress 6 Percent Chironomidae the percent abundance of individuals in the sample that are Chironomidae (non-biting midge) larvae and pupae The best standard value (BSV) is 16 Score = 100 x [(100-X)(100-16)] Percent Chironomidae increases with stress 7 Percent Top Two Dominant the percent abundance of the two most frequent taxa present in the sample The best standard value (BSV) is 328 Score = 100 x [(100-X)(100-328)] The Percent Top 2 Dominant increases with stress 8 Hilsenhoff Biotic Index (HBI) an abundance-weighted average pollution tolerance of all the individuals within a sample keyed to the family level Tolerance ratings range from 1 (highly intolerant) to 10 (highly tolerant) The best standard value (BSV) is 29 Score = 100 x [(10-X)(10-29)] The Hilsenhoff Biotic Index (HBI) increases with stress ______________________________________________________________________________ Table 13 Description of Benthic Macroinvertebrate VSCI Metrics Includes responses (increases decreases) of each VSCI metric to degraded water quality conditions Adapted from Burton and Gerritsen (2003 pp 3-15 3-16 6-2) and DEQ (2008)
q
Appendix C
LABORATORY BENCH SHEETS
Shenandoah Universityrsquos Environmental Studies Department developed the Benthic Macroinvertebrate Laboratory Bench Sheet and VSCI Worksheet to record BMI specimen identifications and their VSCI classifications for each sample
This form is based in part on bench sheets developed by the EPA and Virginia DEQ for recording specimen identifications Additional columns on Shenandoah Universityrsquos forms for relative numbers and VSCI classifications make the calculation and verification of VSCI scores easier
Note the column for taxonomistrsquos initials Specimens were identified by students in Shenandoah Universityrsquos research group and then verified or where necessary changed by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality This external review helps assure the validity of the study Originals of these bench sheets with handwritten taxonomistsrsquo initials will be kept on file at Shenandoah Universityrsquos Environmental Studies Department for five years after the completion of this study
r
s
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-1 STATION NAME Mouth LAT N 39deg 12749rsquo (upstream) LONG W 078deg 04209rsquo (upstream) LAT N 39deg 12729rsquo (downstream) LONG W 078deg 04148rsquo (downstream) FORM COMPILED BY Jeremy Tovar W S Bousquet RIVER BASIN Potomac River INVESTIGATORS Marie Dahl Brandon Millholand J W Pangle Sean Robertson Jeremy Tovar Woodward Bousquet Ben Bousquet
Collection date 21 May 2008 Sheet completion date 12 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Hirudinea 3 33 JT BVW PR 8 SP Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Coenagrionidae 2 22 BVW PR 9 CR Ephemeroptera (mayflies) Caenidae 1 11 JT BVW CG 6 SP Ephemerllidae 4 44 JT BVW CG 2 CR Heptageniidae 5 55 JT BVW SC 3 CG Isonychiidae 1 11 BVW CF 3 CG Plecoptera (stoneflies) Perlidae 14 154 JT BVW PR 2 CR Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Corydalidae 1 11 JT BVW PR 5 CR Coleoptera (beetles) Elmidae 24 264 I JT BVW SC 4 CG Dytiscidae 4 44 JT BVW PR 6 GN Psephenidae 3 33 JT BVW SC 4 CG Diptera (true flies) Tipulidae 3 33 JT BVW SH 4 BU Chironomidae 26 286 JT BVW CG 6 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
t
Other Total Individuals 91 1001 Total Taxa 13
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
u
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-1 STATION NAME Mouth LAT N 39deg 12749rsquo (upstream) LONG W 078deg 04209rsquo (upstream) LAT N 39deg 12729rsquo (downstream) LONG W 078deg 04148rsquo (downstream) FORM COMPILED BY Jeremy Tovar W S Bousquet RIVER BASIN Potomac River INVESTIGATORS Brandon Millholand JW Pangle Jeremy Tovar Heather Kepple
Collection date 10 October 2008 Sheet completion date 12 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Oligochaeta 3 35 JT BVW CG 8 BU Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Coenagrionidae 2 23 JT BVW PR 9 CR Ephemeroptera (mayflies) Heptageniidae 12 140 JT BVW SC 3 CG Isonychiidae 2 23 BVW CF 3 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Hydropsychidae 16 186 JT BVW CF 6 CG Philopotamidae 31 360 JT BVW CF 2 CG Hemiptera (true bugs) (Veliidae) (5) - JT BVW ( ) Megaloptera (dobsonflies etc) Corydalidae 10 116 JT BVW PR 5 CR Coleoptera (beetles) Dytiscidae 1 12 JT BVW PR 6 GN Psephenidae 7 81 JT BVW SC 4 CG Diptera (true flies) Tipulidae 2 23 BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
v
Other Total Individuals 86
(91) 999
Total Taxa 10 (11)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009 Veliidae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
w
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-2 STATION NAME Package Plant LAT N 39deg 12958rsquo (upstream) LONG W 078deg 04900 (upstream) LAT N 39deg 12958rsquo (downstream) LONG W 078deg 04822 (downstream) FORM COMPILED BY JW Pangle W Bousquet RIVER BASIN Potomac River INVESTIGATORS JW Pangle S Robertson J Tovar M Dahl B Millholland W Bousquet
Collection date 29 May 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Plecoptera (stoneflies) Perlidae 11 136 I JP BVW PR 1 CR Trichoptera (caddisflies) Hydropsychidae 10 123 I JP BVW CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 14 173 I JP BVW SC 4 CG Diptera (true flies) Chironomidae 32 395 I JP BVW CG 6 BU Simuliidae 14 173 I JP BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
x
Other Total Individuals 81 1000 Total Taxa 5
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-2 STATION NAME Package Plant LAT N 39deg 12958rsquo (upstream) LONG W 078deg 04900 (upstream) LAT N 39deg 12958rsquo (downstream) LONG W 078deg 04822 (downstream) FORM COMPILED BY JW Pangle W Bousquet RIVER BASIN Potomac River INVESTIGATORS JW Pangle Brandon Millholland Jeremy Tovar Heather Kepple
Collection date 10 October 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Heptageniidae 40 392 I WBJP SC 3 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Hydropsychidae 7 69 I JP WB BVW CF 6 CG Philopotamidae 28 275 I JP WB BVW CF 2 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Corydalidae 6 59 I JP WB BVW PR 5 CR Coleoptera (beetles) Psephenidae 7 69 I JP WB BVW SC 4 CG Elmidae 8 78 I JP WB BVW SC 4 CG Diptera (true flies) Tipulidae 6 59 I JP WB BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
y
z
Other Total Individuals 102 1001 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-3 STATION NAME Rt 664 Bridge LAT N 39deg 12934rsquo (upstream) LONG W 078deg 05019rsquo (upstream) LAT N 39deg 12951rsquo (downstream) LONG W 078deg 04958rsquo (downstream) FORM COMPILED BY S Robertson W Bousquet RIVER BASIN Potomac River INVESTIGATORS SRobertson BMillholland WBousquet MDahl JPangle
Collection date 28 May 2008 Sheet completion date 11 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Leptophlebiidae 1 15 I SGR BVW CG 2 CR Baetidae 1 15 I BVW CG 5 CG Plecoptera (stoneflies) Perlidae 3 46 I SGR BVW PR 1 CR Nemouridae 1 15 I BVW SH 3 CR Trichoptera (caddisflies) Hydropsychidae 5 77 I SGR BVW CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 27 415 I SGR BVW SC 4 CG Psephenidae 2 31 A SGR BVW SC 4 CG Diptera (true flies) Simuliidae 5 77 I SGR BVW CF 6 CG Chironomidae 20 308 I SGR BVW CG 6 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
aa
bb
Other Total Individuals 65 999 Total Taxa 9
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-3 STATION NAME Rt 664 Bridge LAT N 39deg 12934rsquo (upstream) LONG W 078deg 05019rsquo (upstream) LAT N 39deg 12951rsquo (downstream) LONG W 078deg 04958rsquo (downstream) FORM COMPILED BY S Robertson RIVER BASIN Potomac River INVESTIGATORS S Robertson M Dahl A Fisher H Kepple
Collection date 10 Oct 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Heptageniidae 2 20 I SGR SC 3 CG Isonychiidae 2 20 I BVW CF 2 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Philopotamidae 36 367 I SGR CF 2 CG Hydropsychidae 47 480 I SGR CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 2 20 A SGR SC 4 CG Psephenidae 8 82 A SGR SC 4 CG Diptera (true flies) Tabanidae 1 10 I SGR PR 7 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
cc
dd
Other Total Individuals 98 999 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-4 STATION NAME Unnamed Tributary LAT (upstream) N 39deg 13035rsquo LONG (upstream) W 078deg 05129rsquo LAT (downstream) N 39deg 13000rsquo LONG (downstream) W 078deg 5086rsquo FORM COMPILED BY A Fisher M Dahl rev W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson A Fisher H Kepple
Collection date 31 May 2008 Sheet completion date rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Flatworms (Turbellaria) Worms (Oligochaeta) Naididae 1 14 - BVW CG 8 BU Leeches (Hirudinea) Snails (Gastropoda) Clams (Bivalvia) Isopods (Isopoda) Asellidae 3 42 A AF BVW CG 8 SP Scuds (Amphipoda) Crayfish Shrimp (Decapoda) Water Mites (Hydracarina) Dragonflies (Odonata Anisoptera) Damselflies (Odonata Zygoptera) Mayflies (Ephemeroptera) Stoneflies (Plecoptera) Perlidae 2 28 I AF BVW PR 1 CR Caddisflies (Trichoptera) True Bugs (Hemiptera) Corixidae 1 14 I AF BVW MP 9 DV Dobsonflies (Megaloptera) Beetles (Coleoptera) Dytiscidae 1 14 I AF BVW PR 6 GN True Flies (Diptera) Chironomidae 61 847 I AF BVW CG 6 BU Simuliidae 3 42 I AF BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
ee
ff
Other Total Individuals 72 1001 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-4 STATION NAME Unnamed Tributary LAT (upstream) N 39deg 13035rsquo LONG (upstream) W 078deg 05129rsquo LAT (downstream) N 39deg 13000rsquo LONG (downstream) W 078deg 5086rsquo FORM COMPILED BY Andrew G M Fisher W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson A Fisher H Kepple
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Flatworms (Turbellaria) Worms (Oligochaeta) Lumbriculidae 2 32 A AF BVW CG 8 BU Leeches (Hirudinea) Snails (Gastropoda) Lymnaeidae 1 16 A BVW CG 9 GN Clams (Bivalvia) Isopods (Isopoda) Scuds (Amphipoda) Crayfish Shrimp (Decapoda) Cambaridae 1 16 A AF BVW GN 5 GN Water Mites (Hydracarina) Dragonflies (Odonata Anisoptera) Damselflies (Odonata Zygoptera) Calopterygidae 4 63 I BVW PR 7 CL Mayflies (Ephemeroptera) Tricorythidae 1 16 I BVW CG 5 SP Stoneflies (Plecoptera) Caddisflies (Trichoptera) Phryganeidae 4 63 I AF BVW SH 4 CR Hydropsychidae 6 95 I AF BVW CF 6 CG I True Bugs (Hemiptera) Corixidae 8 127 I AF BVW MP 9 DV (Veliidae ) (7) - I AF BVW ( ) Dobsonflies (Megaloptera) Sialidae 3 48 I AF BVW PR 7 SP Corydalidae 2 32 I AF BVW PR 5 CR Beetles (Coleoptera) Haliplidae 1 16 A AF BVW MP 8 GN Dysticidae 7 111 I AF BVW PR 6 GN Hydrophilidae 1 16 A AF BVW PR 8 GN Elmidae 3 48 I BVW SC 4 CG True Flies (Diptera) Chironomidae 8 127 I AF BVW CG 6 BU Simuliidae 7 111 I AF BVW CF 6 CG Tipulidae 4 63 I AF BVW SH 4 GU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
gg
hh
Other Total Individuals 63
(70) 1000
Total Taxa 17 (18)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Veliidae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-5 STATION NAME Lick Run LAT N 39deg 12752rsquo (upstream) LONG W 078deg 05685rsquo (upstream) LAT N 39deg 12784rsquo (downstream) LONG W 078deg 05631rsquo (downstream) FORM COMPILED BY Brandon Millholland W Bousquet RIVER BASIN Potomac River INVESTIGATORS J Pangle S Robertson W Bousquet JTovar M Dahl
Collection date 29 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Gomphidae 1 15 I BVW PR 3 BU Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Leptophlebiidae 11 167 A LBM BVW CG 2 CR Plecoptera (stoneflies) Perlidae 10 152 A LBM BVW PR 1 CR Perlodiae 1 15 A LBM BVW PR 2 CR Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 5 76 A LBM BVW SC 4 CG Elmidae 2 30 I LBM BVW SC 4 CG Diptera (true flies) (Culicidae) (3) - P LMB BVW ( ) Chironomidae 23 348 I LBM BVW CG 6 BU Simuliidae 13 197 I LBM BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
ii
jj
Other Total Individuals 66
(69) 1000
Total Taxa 7 (8)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Culicidae (Diptera) pupae not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl J Pangle S Robertson W Bousquet B Millholland J Tovar H Kepple
Collection date 30 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 1 12 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Asellidae 114 965 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Plecoptera (stoneflies) Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 2 23 A MRD BVW SC 4 CG Diptera (true flies)
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
kk
ll
Other Total Individuals 117 1000 Total Taxa 3
Notes Subsample taken 29 October 2009 All taxonomic determinations by MRD were confirmed by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson K McCann A Fisher H Kepple A Barrett
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 2 17 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Sphaeriidae 2 17 I MRD BVW CF 8 BU Isopoda (isopods) Asellidae 99 853 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Calopterygidae 2 17 A MRD BVW PR 7 CL Ephemeroptera (mayflies) Leptophlebiidae 2 17 I MRD BVW CG 2 CR Heptageniidae 4 34 A MRD BVW SC 3 CG Plecoptera (stoneflies) Perlidae 1 09 A MRD BVW PR 1 CR Trichoptera (caddisflies) Hemiptera (true bugs) (Gerridae) (1) - A MRD BVW ( ) Corixidae 1 09 A MRD BVW PR 5 CR Megaloptera (dobsonflies etc) Coleoptera (beetles) Dryopidae 1 09 A MRD BVW SC 5 CG Diptera (true flies) Tipulidae 2 17 I BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
mm
nn
Other Total Individuals 116
(117) 999
Total Taxa 10 (11)
Notes Subsample taken 6 November 2008 Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009 Gerridae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
- Disclaimer ii
- Title Page iii
- Acknowledgements v
- Table of Contents vii
- SECTION 2 RESULTS DATA TABLES 7
- OVERVIEW AND FINDINGS
- Introduction
- RESULTS DATA TABLES
- REFERENCES
- FIELD DATA SHEETS
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- SubstrateType
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- WATER QUALITY ANALYSIS
- THROUGH THE VIRGINIA
- STREAM CONDITION INDEX (VSCI)
- LABORATORY BENCH SHEETS
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
REFERENCES
Barbour Michael T Jeroen Gerritsen Blaine D Snyder and James B Stribling 1999 Rapid bioassessment protocols for use in streams and wadeable rivers periphyton benthic macroinvertebrates and fish Second edition EPA 841-B-99-002 Washington DC US Environmental Protection Agency Office of Water Available online at httpwwwepagovOWOWmonitoringbioasspshtml Burton June and Jeroen Gerritsen 2003 A stream condition index for Virginia non-coastal streams Prepared for the US EPA Office of Science and Technology US EPA Region 3 Environmental Services Division and Virginia Department of Environmental Quality Owings Mills Maryland Tetra Tech Inc Available online at httpwwwdeqvirginiagovwatermonitoringpdf vastrmconpdf Hessler Rachel A Andrea R Martin Heidi D Ruggeri William B Seymour Jr and Woodward S Bousquet 2006 Water quality assessment using benthic macroinvertebrates A study and compilation Winchester Virginia Environmental Studies Program Shenandoah University Occasional publication no 10 Igoe Robert 2006 DEQ identifies nine impaired streams in Clarke Frederick The Winchester Star 15 July B1 Jackson Charlie 2005 Survey targets Opequon cleanup The Winchester Star 19 August B1 Johnston Elizabeth A and Woodward S Bousquet 2003 What happens when it rains A study of water quality in Abrams Creek and Town Run Winchester and Frederick County Virginia Winchester Virginia Environmental Studies Program Shenandoah University Occasional publication no7 Merritt Richard W and Kenneth W Cummins 1996 An introduction to the aquatic insects of North America Dubuque Iowa KendallHunt Publishing Company Opequon Creek IP Steering Committee Virginia Tech Department of Biological Systems Engineering and Center for TMDL and Watershed Studies University of Virginia Institute for Environmental Negotiation and West Virginia University Division of Resource Management 2006 Opequon Creek watershed TMDL implementation plan Blacksburg Virginia Virginia Tech Department of Biological Systems Engineering Available online at httpwwwtmdlbsevteduoutreachC82 Pennak Robert W 1953 Fresh-water invertebrates of the United States New York New York The Ronald Press Company Rosenburg David M and Vincent H Resh 1993 Freshwater biomonitoring and benthic macroinvertebrates New York New York Chapman amp Hall Shenandoah University Public Relations Office 2002 Data collected by SU and its partners helps identify impaired water Winchester Virginia Available online at httpwwwsuedutemp_ newscfmurlnum=32 Thorp James H and Alan P Covich 1991 Ecology and classification of North American freshwater invertebrates San Diego California Academic Press Inc
13
US Environmental Protection Agency 1997 Volunteer stream monitoring a methods manual EPA 841-B-97-003 Washington DC US Environmental Protection Agency Office of Water Van Meter Val 2003 Opequon Creek pollution limits to be established The Winchester Star 4 April B1 Virginia Department of Environmental Quality 2006a Virginia 305(b)303(d) water quality integrated report to Congress and the EPA Administrator for the period January 1 2000 to December 31 2004 Richmond Virginia Virginia Department of Environmental Quality Available online at httpwwwdeqvirginiagovwqair2006html __________ 2006b Using probabilistic data to validate the non-coastal Virginia Stream Condition Index (draft) VDEQ technical bulletin WQA2006-001 Virginia Department of Environmental Quality Available online at httpwwwloudounwatershedorgstream_assessmentreference_documents VA_DEQ_SCI_Validationpdf __________ 2008 Biological monitoring program quality assurance project plan for wadeable streams and rivers Richmond Virginia Virginia Department of Environmental Quality Update available online at httpwwwdeqstatevausbiomonitoringdocumentsBioMonQAPP_13Aug2008pdf Virginia Tech Aquatic Entomology Laboratory 2005 Taxa dictionary [Excel file] Blacksburg Virginia Virginia Tech Department of Biological Systems Engineering 2003a Opequon watershed TMDLs for benthic impairments Abrams Creek and Lower Opequon Creek Frederick and Clarke Counties Virginia Prepared for the Virginia Department of Environmental Quality and Virginia Department of Conservation and Recreation Blacksburg Virginia Available online at httpwwwdeqstate vaustmdlapptmdlsshenrvrabropebcpdf Voshell J Reese Jr 2002 A guide to common freshwater invertebrates of North America Blacksburg Virginia The McDonald amp Woodward Publishing Company
14
APPENDICES
15
16
a
Appendix A
FIELD DATA SHEETS
This appendix presents a Site Characterization Field Data Sheet for each of the six sampling reaches established on Hiatt Run and Lick Run for this study They were filled out during the sampling runs made in May 2008
These forms were adapted by Shenandoah University from forms used by the EPA and Virginia DEQ for recording characteristics of sampling sites
b
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Mouth STATION _HLR-1_____ STREAM CLASS LAT N 39 12749 (upstream) LONG W 078 04209(upstream) LAT N 39 12729 (downstream) LONG W 078 04148 (downstream) FORM COMPILED BY Jeremy Tovar RIVER BASIN Potomac River INVESTIGATORS JPangle B Millholland MDahl SRobertson W Bousquet JTovar BBousquet
DATE May 21 2008_________ TIME_250 PM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature __24___degC showers (intermittent) Comments _______________________
___ cloud cover _0_ ______________________________
X clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x ( Significant springs andor seeps) x (Valley ) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS
c
d
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence x FieldPasture Industrial Obvious sources Agricultural Other ____________ Stream scour steep slope erosion x Residential Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left Field and young thin deciduous forest 100 Bank=Grass cobble and shrub Right 90 maturing deciduous forest 45 shale slope Bank=Bedrock soil and herbs Indicate the dominant vegetation type(s) and record the dominant species
x Trees Shrubs x (Grasses) Forbs Dominant species Oak species and mix deciduous
INSTREAM FEATURES
Measured reach length ___100__m Canopy Cover Estimated stream width _35__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area __350_m2
Estimated stream depth _008_m Proportion of Reach Represented by Stream High water mark _15_m Morphology Types Surface velocity (at thalweg) _05__msec x Riffle__75___ x Run__20___ x Pool__5___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___00__ m2 Density of LWD _-_ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __15_____ x Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present Algae
WATER QUALITY Temperature __19___degC Water Odors x NormalNone Sewage Specific Conductance - Petroleum Chemical Fishy Other___________________ Dissolved Oxygen - Water Surface Oils Slick Sheen Globs Flecks pH __-_ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used _____NA________ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic x None Relict shells Other______None_____ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 8 Detritus sticks wood coarse plant materials
0 Boulder gt256 mm (10rsquorsquo) 0
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 51 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 40 Sand 006-2 mm (gritty) 1 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University
SITE CHARACTERIZATION FIELD DATA SHEET FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Package Plant STATION HLR-2 STREAM CLASS LAT N 39 129580 (upstream) LONG W 078 04900 (upstream) LAT N 39 129581 (downstream) LONG W 078 04822 (downstream) FORM COMPILED BY JW Pangle RIVER BASIN Potomac River INVESTIGATORS JPangle JTovar MDahl B Millholland SRobertson W Bousquet
DATE__19 May 2008_ TIME__1240__ PM
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature _____degC showers (intermittent) Comments __Sunny Day_____________
_5__ cloud cover _0__ ______________________________
x clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x (Significant springs andor seeps) x (Valley) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS
e
f
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence FieldPasture Industrial Obvious sources Agricultural Other ____________ __Upstream Construction and Stream bank Scour Residential __________________________________ Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left Deciduous forest with thick herb and shrub layers bank= vegetation and rocks Right Deciduous forest with thick herb and shrub layers bank=vegetation soil and rocks Indicate the dominant vegetation type(s) and record the dominant species
x Trees x Shrubs Grasses Forbs Dominant species Black Walnut White Ash Hackberry and American Sycamore
INSTREAM FEATURES
Measured reach length 100 m Canopy Cover Estimated stream width _35__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area __350_m2
Estimated stream depth __15__m Proportion of Reach Represented by Stream High water mark __22___m Morphology Types Surface velocity (at thalweg) _035__msec x Riffle__30___ x Run__30___ x Pool__40___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___10____ m2 Density of LWD _______ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __30___ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present__________________________________________________________
WATER QUALITY Temperature __18___degC Water Odors x NormalNone Sewage Specific Conductance __-____ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ____-____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used _____NA____ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other___None_____ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach SubstrateType Characteristic
Composition in Sampling Reach
Bedrock 3 Detritus sticks wood coarse plant materials
2 Boulder gt256 mm (10rsquorsquo) 5
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 70 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 22 Sand 006-2 mm (gritty) 0 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiattrsquos Run LOCATION Rt 664 Bridge STATION HLR-3 STREAM CLASS LAT N 39 12934 (upstream) LONG W 078 05019 (upstream) LAT N 39 12958 (downstream) LONG W 078 04958 (downstream) FORM COMPILED BY Sean Robertson RIVER BASIN Potomac River INVESTIGATORS SRobertson BMillholland WBousquet MDahl JPangle
DATE 28 May 2008 TIME 1127 AM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality 2008
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature __14___degC showers (intermittent) x Trace Comments _______________________
_65 x cloud cover ___ ______________________________
x clearsunny __________________________________
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x (Significant springs andor seeps) x (Valley) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS
g
h
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence Some evidence x FieldPasture Industrial x Obvious sources Agricultural x Other Old Package Plant Downstream x Residential Bridge and Road Power line and Pump station Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left 70 field 30 shrubs and deciduous trees Bank=Soil and vegetation Right Maturing deciduous forest steep slope Bank=Shale Bedrock Indicate the dominant vegetation type(s) and record the dominant species
x Trees x Shrubs x Grasses Forbs Dominant species Sycamore and Red Oaks
INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width __51___m Fully open x Partly open Partly shaded Fully shaded Sampling reach area _510_m2
Estimated stream depth __135___m Proportion of Reach Represented by Stream High water mark __-___m Morphology Types Surface velocity (at thalweg) __20___msec x Riffle_40_ x Run_50_ x Pool 10 Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___0____ m2 Density of LWD ___-____ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation ___50____ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present Brown Algae
WATER QUALITY Temperature __17___degC Water Odors x NormalNone Sewage Specific Conductance ___-___ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ___-_____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used ____NA_______ Clear x Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other____None____ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 15 Detritus sticks wood coarse plant materials
0 Boulder gt256 mm (10rsquorsquo) 15
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 62 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 8 Sand 006-2 mm (gritty) 0 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Unnamed Tributary STATION HLR-4 STREAM CLASS LAT N 39 13035 (upstream) LONG W 078 05129 (upstream) LAT N 39 13000 (downstream) LONG W 078 05086 (downstream) FORM COMPILED BY Marie Dahl RIVER BASIN Potomac River INVESTIGATORS M Dahl HKepple SRobertson AFisher
DATE 31 May 2008 TIME 5 PM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality 2008
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) YES x NO
rain (steady rain) Air Temperature __22___degC showers (intermittent) Comments _______________________
_0__ cloud cover _0__ ______________________________
x clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area __-___ km2 mi2 Montane Significant springs andor seeps x Valley Marsh or swamp Montane amp Valley Mixture of origins Other ___________________
COMMENTS Snowden Bridge development (upstream) and sewer line
i
j
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence x FieldPasture Industrial Obvious sources Agricultural Other ____________ Construction upstream and adjacent x Residential Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left Forested with rocky banks field across silt fence and 2m tree strip Right Forested with some rocky banks and forest through the highway Indicate the dominant vegetation type(s) and record the dominant species
x Trees x Shrubs x Grasses Forbs Dominant species White Ash and Hackberry
INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width ___38__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area __380__m2
Estimated stream depth __12__cm Proportion of Reach Represented by Stream High water mark __2___m Morphology Types Surface velocity (at thalweg) __46___msec x Riffle__25___ x Run__70___ x Pool__5___ Channelized Yes x No Dam Present x Yes No
LARGE WOODY DEBRIS
LWD _______ m2 Density of LWD _______ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation ___NA____ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present___Algae___
WATER QUALITY Temperature __16___degC Water Odors x NormalNone Sewage Specific Conductance __-____ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ___-_____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used ____NA_____ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells x Other___Shale_______ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 0 Detritus sticks wood coarse plant materials
10 Boulder gt256 mm (10rsquorsquo) 5
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 45 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 30 Sand 006-2 mm (gritty) 0 Marl grey shell
fragments 0
Silt 0004-006 mm 20 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Lick Run STATION HLR-5 STREAM CLASS LAT N 39 127517 (upstream) LONG W 078 05685(upstream) LAT N 39 12784 (downstream) LONG W 078 05631 (downstream) FORM COMPILED BY B Millholland RIVER BASIN Potomac River INVESTIGATORS B Millholland JTovar SRobertson MDahl WBousquet JPangle
DATE 29 May 2008 TIME 1040 AM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project
k
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature __15___degC showers (intermittent) Comments _______________________
_5_ X cloud cover _0__ ______________________________
x clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane Significant springs andor seeps x Valley Marsh or swamp Montane amp Valley Mixture of origins Other ___________________
COMMENTS Lick Run appears to be intermittent and may be dry in Fall 2008
l
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence x FieldPasture Industrial Obvious sources Agricultural Other ____________ Adjacent logging stream bank scour Residential Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left 85 young deciduous forest 15 logged Bank=Soil vegetation and cobbles Right Young deciduous forest on 30 slope Bank= Soil vegetation and cobble Indicate the dominant vegetation type(s) and record the dominant species
x Trees Shrubs Grasses Forbs Dominant species Mixed Deciduous INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width __11__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area 110 m2
Estimated stream depth _009__m Proportion of Reach Represented by Stream High water mark __96___m Morphology Types Surface velocity (at thalweg) __01___msec x Riffle__30___ x Run__5___ x Pool__65___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___0____ m2 Density of LWD ___-____ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __70___ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present__________________________________________________________
WATER QUALITY Temperature __14___degC Water Odors x NormalNone Sewage Specific Conductance __-__ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ___-___ Water Surface Oils Slick Sheen Globs Flecks pH __-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used _____NA______ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other_____None_____
SEDIMENT AND SUBSTRATE
Odors Deposits Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other________________ x Other Slightly Salty Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 0 Detritus sticks wood coarse plant materials
5 Boulder gt256 mm (10rsquorsquo) 0
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 75 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 23 Sand 006-2 mm (gritty) 2 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION McCann Farm STATION HLR-6 STREAM CLASS LAT N 39 13076 (upstream) LONG W 078 06539 (upstream) LAT N 39 13063 (downstream) LONG W 078 06473 (downstream) FORM COMPILED BY MDahl RIVER BASIN Potomac River INVESTIGATORS MDahl JPangle SRobertson W Bousqet BMillholland JTovar H Kepple
DATE 30 May 2008 TIME 1050 AM (start)
PROJECT NAME
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) YES x NO
rain (steady rain) Air Temperature __18___degC showers (intermittent) Comments _______________________
_85_x cloud cover _10__ ______________________________
clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x (Significant springs andor seeps) x ( Valley) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS Multi-habitat sampling with D-frame nets and seine
m
n
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial x No evidence Some evidence x FieldPasture Industrial Obvious sources x Agricultural Other ____________ Minor stream bank scour Residential Local Watershed Erosion x None Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left 35 bank grasses scattered trees and some bushes Right High bank with grasses and scattered trees Indicate the dominant vegetation type(s) and record the dominant species
x Trees Shrubs Grasses Forbs Dominant species Black Walnut and Festuca elatior
INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width __2___m Fully open Partly open x Partly shaded Fully shaded Sampling reach area __200_m2
Estimated stream depth __03_m Proportion of Reach Represented by Stream High water mark __1___m Morphology Types Surface velocity (at thalweg) __166_msec x Riffle__0___ x Run__55___ x Pool__45___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___2____ m2 Density of LWD ___-____ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __20_____ x Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present ____Glyceria (grass)sp__________
WATER QUALITY Temperature __16___degC Water Odors x NormalNone Sewage Specific Conductance ___-___ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ____-____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used ______NA___ Clear x Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other__NONE__ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 0 Detritus sticks wood coarse plant materials
5 Boulder gt256 mm (10rsquorsquo) 0
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 27 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 38 Sand 006-2 mm (gritty) 5 Marl grey shell
fragments 0 (lt1)
Silt 0004-006 mm 25 Clay lt 0004 mm (slick) 5 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Appendix B
WATER QUALITY ANALYSIS THROUGH THE VIRGINIA
STREAM CONDITION INDEX (VSCI)
The Virginia Stream Condition Index (VSCI) relies on eight metrics to categorize stream health According to Burton and Gerritsen (2003 p 1-1) this index ldquohellip can be used as a primary indicator of ecosystem health and can identify impairment with respect to the reference (or natural) conditionrdquo These metrics are numeric measures of a biological communityrsquos species composition diversity ecological niches or tolerance to pollution
Table 13 on the next page describes each VSCI metric lists its formula and provides the best standard value (BSV) for that metric Based on a 100-point scale the BSV is the score that reflects optimum conditions for aquatic life ie the best water quality value possible (DEQ 2006b pp 8 33) The table also explains whether the score increases or decreases with environmental stress
As Table 13 indicates each of the eight VSCI metrics is calculated individually Each formula provides a unitless score based on a 100-point scale with 0 as the worst possible score and 100 as the best Any metric that provides a result above 100 is changed to 100 in order for the index to work properly After the eight metrics are calculated they are summed and averaged to provide a final VSCI score on a 100-point scale
Based on statistical analysis of Virginiarsquos streams and their BMI communities the DEQ (2006b p 8) currently uses the following tiers to interpret VSCI scores
VSCI Scores Aquatic Life Use
(ALI) Tiers
lt 42 severely stressed
42-55 moderately stressed
55-63 unknownfair (gray zone)
63-73 good
gt73 excellent
Section 2 of this report presents the results of calculations for the six BMI sampling sites the research team studied in the Hiatt-Lick Run subwatershed in 2008
o
p
1 Total taxa the number of distinct taxa in the sample measures the overall diversity of the macroinvertebrate assemblage The best standard value (BSV) is 189 Score = 100 x (X189) Total taxa decreases with stress 2 EPT taxa the sum of the distinct taxa in the orders Ephemeroptera (mayflies) Plecoptera (stoneflies) and Trichoptera (caddisflies) These orders are known to be sensitive to pollution The best standard value (BSV) is 117 Score = 100 x (X117) EPT taxa decreases with stress 3 Percent Ephemeroptera the percent abundance of individuals in the sample that belong to the order Ephemeroptera (mayflies) The best standard value (BSV) is 477 Score = 100 x (X477) Percent Ephemeroptera decreases with stress 4 Percent Plecoptera + Trichoptera less Hydropsychidae the percent abundance of individuals in the sample that belong to the orders Plecoptera (stoneflies) and Trichoptera (caddisflies) excluding the pollution-tolerant caddisfly family Hydropsychidae The best standard value (BSV) is 562 Score = 100 x (X562) Percent Plecoptera + Trichoptera less Hydropsychidae decreases with stress 5 Percent Scrapers the percent abundance of individuals in the sample whose primary functional mechanism for feeding is grazing on the substrate or periphyton (ie attached algae and associated material) The best standard value (BSV) is 467 Score = 100 x (X467) Percent scrapers decreases with stress 6 Percent Chironomidae the percent abundance of individuals in the sample that are Chironomidae (non-biting midge) larvae and pupae The best standard value (BSV) is 16 Score = 100 x [(100-X)(100-16)] Percent Chironomidae increases with stress 7 Percent Top Two Dominant the percent abundance of the two most frequent taxa present in the sample The best standard value (BSV) is 328 Score = 100 x [(100-X)(100-328)] The Percent Top 2 Dominant increases with stress 8 Hilsenhoff Biotic Index (HBI) an abundance-weighted average pollution tolerance of all the individuals within a sample keyed to the family level Tolerance ratings range from 1 (highly intolerant) to 10 (highly tolerant) The best standard value (BSV) is 29 Score = 100 x [(10-X)(10-29)] The Hilsenhoff Biotic Index (HBI) increases with stress ______________________________________________________________________________ Table 13 Description of Benthic Macroinvertebrate VSCI Metrics Includes responses (increases decreases) of each VSCI metric to degraded water quality conditions Adapted from Burton and Gerritsen (2003 pp 3-15 3-16 6-2) and DEQ (2008)
q
Appendix C
LABORATORY BENCH SHEETS
Shenandoah Universityrsquos Environmental Studies Department developed the Benthic Macroinvertebrate Laboratory Bench Sheet and VSCI Worksheet to record BMI specimen identifications and their VSCI classifications for each sample
This form is based in part on bench sheets developed by the EPA and Virginia DEQ for recording specimen identifications Additional columns on Shenandoah Universityrsquos forms for relative numbers and VSCI classifications make the calculation and verification of VSCI scores easier
Note the column for taxonomistrsquos initials Specimens were identified by students in Shenandoah Universityrsquos research group and then verified or where necessary changed by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality This external review helps assure the validity of the study Originals of these bench sheets with handwritten taxonomistsrsquo initials will be kept on file at Shenandoah Universityrsquos Environmental Studies Department for five years after the completion of this study
r
s
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-1 STATION NAME Mouth LAT N 39deg 12749rsquo (upstream) LONG W 078deg 04209rsquo (upstream) LAT N 39deg 12729rsquo (downstream) LONG W 078deg 04148rsquo (downstream) FORM COMPILED BY Jeremy Tovar W S Bousquet RIVER BASIN Potomac River INVESTIGATORS Marie Dahl Brandon Millholand J W Pangle Sean Robertson Jeremy Tovar Woodward Bousquet Ben Bousquet
Collection date 21 May 2008 Sheet completion date 12 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Hirudinea 3 33 JT BVW PR 8 SP Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Coenagrionidae 2 22 BVW PR 9 CR Ephemeroptera (mayflies) Caenidae 1 11 JT BVW CG 6 SP Ephemerllidae 4 44 JT BVW CG 2 CR Heptageniidae 5 55 JT BVW SC 3 CG Isonychiidae 1 11 BVW CF 3 CG Plecoptera (stoneflies) Perlidae 14 154 JT BVW PR 2 CR Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Corydalidae 1 11 JT BVW PR 5 CR Coleoptera (beetles) Elmidae 24 264 I JT BVW SC 4 CG Dytiscidae 4 44 JT BVW PR 6 GN Psephenidae 3 33 JT BVW SC 4 CG Diptera (true flies) Tipulidae 3 33 JT BVW SH 4 BU Chironomidae 26 286 JT BVW CG 6 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
t
Other Total Individuals 91 1001 Total Taxa 13
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
u
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-1 STATION NAME Mouth LAT N 39deg 12749rsquo (upstream) LONG W 078deg 04209rsquo (upstream) LAT N 39deg 12729rsquo (downstream) LONG W 078deg 04148rsquo (downstream) FORM COMPILED BY Jeremy Tovar W S Bousquet RIVER BASIN Potomac River INVESTIGATORS Brandon Millholand JW Pangle Jeremy Tovar Heather Kepple
Collection date 10 October 2008 Sheet completion date 12 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Oligochaeta 3 35 JT BVW CG 8 BU Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Coenagrionidae 2 23 JT BVW PR 9 CR Ephemeroptera (mayflies) Heptageniidae 12 140 JT BVW SC 3 CG Isonychiidae 2 23 BVW CF 3 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Hydropsychidae 16 186 JT BVW CF 6 CG Philopotamidae 31 360 JT BVW CF 2 CG Hemiptera (true bugs) (Veliidae) (5) - JT BVW ( ) Megaloptera (dobsonflies etc) Corydalidae 10 116 JT BVW PR 5 CR Coleoptera (beetles) Dytiscidae 1 12 JT BVW PR 6 GN Psephenidae 7 81 JT BVW SC 4 CG Diptera (true flies) Tipulidae 2 23 BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
v
Other Total Individuals 86
(91) 999
Total Taxa 10 (11)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009 Veliidae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
w
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-2 STATION NAME Package Plant LAT N 39deg 12958rsquo (upstream) LONG W 078deg 04900 (upstream) LAT N 39deg 12958rsquo (downstream) LONG W 078deg 04822 (downstream) FORM COMPILED BY JW Pangle W Bousquet RIVER BASIN Potomac River INVESTIGATORS JW Pangle S Robertson J Tovar M Dahl B Millholland W Bousquet
Collection date 29 May 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Plecoptera (stoneflies) Perlidae 11 136 I JP BVW PR 1 CR Trichoptera (caddisflies) Hydropsychidae 10 123 I JP BVW CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 14 173 I JP BVW SC 4 CG Diptera (true flies) Chironomidae 32 395 I JP BVW CG 6 BU Simuliidae 14 173 I JP BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
x
Other Total Individuals 81 1000 Total Taxa 5
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-2 STATION NAME Package Plant LAT N 39deg 12958rsquo (upstream) LONG W 078deg 04900 (upstream) LAT N 39deg 12958rsquo (downstream) LONG W 078deg 04822 (downstream) FORM COMPILED BY JW Pangle W Bousquet RIVER BASIN Potomac River INVESTIGATORS JW Pangle Brandon Millholland Jeremy Tovar Heather Kepple
Collection date 10 October 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Heptageniidae 40 392 I WBJP SC 3 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Hydropsychidae 7 69 I JP WB BVW CF 6 CG Philopotamidae 28 275 I JP WB BVW CF 2 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Corydalidae 6 59 I JP WB BVW PR 5 CR Coleoptera (beetles) Psephenidae 7 69 I JP WB BVW SC 4 CG Elmidae 8 78 I JP WB BVW SC 4 CG Diptera (true flies) Tipulidae 6 59 I JP WB BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
y
z
Other Total Individuals 102 1001 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-3 STATION NAME Rt 664 Bridge LAT N 39deg 12934rsquo (upstream) LONG W 078deg 05019rsquo (upstream) LAT N 39deg 12951rsquo (downstream) LONG W 078deg 04958rsquo (downstream) FORM COMPILED BY S Robertson W Bousquet RIVER BASIN Potomac River INVESTIGATORS SRobertson BMillholland WBousquet MDahl JPangle
Collection date 28 May 2008 Sheet completion date 11 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Leptophlebiidae 1 15 I SGR BVW CG 2 CR Baetidae 1 15 I BVW CG 5 CG Plecoptera (stoneflies) Perlidae 3 46 I SGR BVW PR 1 CR Nemouridae 1 15 I BVW SH 3 CR Trichoptera (caddisflies) Hydropsychidae 5 77 I SGR BVW CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 27 415 I SGR BVW SC 4 CG Psephenidae 2 31 A SGR BVW SC 4 CG Diptera (true flies) Simuliidae 5 77 I SGR BVW CF 6 CG Chironomidae 20 308 I SGR BVW CG 6 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
aa
bb
Other Total Individuals 65 999 Total Taxa 9
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-3 STATION NAME Rt 664 Bridge LAT N 39deg 12934rsquo (upstream) LONG W 078deg 05019rsquo (upstream) LAT N 39deg 12951rsquo (downstream) LONG W 078deg 04958rsquo (downstream) FORM COMPILED BY S Robertson RIVER BASIN Potomac River INVESTIGATORS S Robertson M Dahl A Fisher H Kepple
Collection date 10 Oct 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Heptageniidae 2 20 I SGR SC 3 CG Isonychiidae 2 20 I BVW CF 2 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Philopotamidae 36 367 I SGR CF 2 CG Hydropsychidae 47 480 I SGR CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 2 20 A SGR SC 4 CG Psephenidae 8 82 A SGR SC 4 CG Diptera (true flies) Tabanidae 1 10 I SGR PR 7 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
cc
dd
Other Total Individuals 98 999 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-4 STATION NAME Unnamed Tributary LAT (upstream) N 39deg 13035rsquo LONG (upstream) W 078deg 05129rsquo LAT (downstream) N 39deg 13000rsquo LONG (downstream) W 078deg 5086rsquo FORM COMPILED BY A Fisher M Dahl rev W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson A Fisher H Kepple
Collection date 31 May 2008 Sheet completion date rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Flatworms (Turbellaria) Worms (Oligochaeta) Naididae 1 14 - BVW CG 8 BU Leeches (Hirudinea) Snails (Gastropoda) Clams (Bivalvia) Isopods (Isopoda) Asellidae 3 42 A AF BVW CG 8 SP Scuds (Amphipoda) Crayfish Shrimp (Decapoda) Water Mites (Hydracarina) Dragonflies (Odonata Anisoptera) Damselflies (Odonata Zygoptera) Mayflies (Ephemeroptera) Stoneflies (Plecoptera) Perlidae 2 28 I AF BVW PR 1 CR Caddisflies (Trichoptera) True Bugs (Hemiptera) Corixidae 1 14 I AF BVW MP 9 DV Dobsonflies (Megaloptera) Beetles (Coleoptera) Dytiscidae 1 14 I AF BVW PR 6 GN True Flies (Diptera) Chironomidae 61 847 I AF BVW CG 6 BU Simuliidae 3 42 I AF BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
ee
ff
Other Total Individuals 72 1001 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-4 STATION NAME Unnamed Tributary LAT (upstream) N 39deg 13035rsquo LONG (upstream) W 078deg 05129rsquo LAT (downstream) N 39deg 13000rsquo LONG (downstream) W 078deg 5086rsquo FORM COMPILED BY Andrew G M Fisher W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson A Fisher H Kepple
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Flatworms (Turbellaria) Worms (Oligochaeta) Lumbriculidae 2 32 A AF BVW CG 8 BU Leeches (Hirudinea) Snails (Gastropoda) Lymnaeidae 1 16 A BVW CG 9 GN Clams (Bivalvia) Isopods (Isopoda) Scuds (Amphipoda) Crayfish Shrimp (Decapoda) Cambaridae 1 16 A AF BVW GN 5 GN Water Mites (Hydracarina) Dragonflies (Odonata Anisoptera) Damselflies (Odonata Zygoptera) Calopterygidae 4 63 I BVW PR 7 CL Mayflies (Ephemeroptera) Tricorythidae 1 16 I BVW CG 5 SP Stoneflies (Plecoptera) Caddisflies (Trichoptera) Phryganeidae 4 63 I AF BVW SH 4 CR Hydropsychidae 6 95 I AF BVW CF 6 CG I True Bugs (Hemiptera) Corixidae 8 127 I AF BVW MP 9 DV (Veliidae ) (7) - I AF BVW ( ) Dobsonflies (Megaloptera) Sialidae 3 48 I AF BVW PR 7 SP Corydalidae 2 32 I AF BVW PR 5 CR Beetles (Coleoptera) Haliplidae 1 16 A AF BVW MP 8 GN Dysticidae 7 111 I AF BVW PR 6 GN Hydrophilidae 1 16 A AF BVW PR 8 GN Elmidae 3 48 I BVW SC 4 CG True Flies (Diptera) Chironomidae 8 127 I AF BVW CG 6 BU Simuliidae 7 111 I AF BVW CF 6 CG Tipulidae 4 63 I AF BVW SH 4 GU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
gg
hh
Other Total Individuals 63
(70) 1000
Total Taxa 17 (18)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Veliidae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-5 STATION NAME Lick Run LAT N 39deg 12752rsquo (upstream) LONG W 078deg 05685rsquo (upstream) LAT N 39deg 12784rsquo (downstream) LONG W 078deg 05631rsquo (downstream) FORM COMPILED BY Brandon Millholland W Bousquet RIVER BASIN Potomac River INVESTIGATORS J Pangle S Robertson W Bousquet JTovar M Dahl
Collection date 29 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Gomphidae 1 15 I BVW PR 3 BU Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Leptophlebiidae 11 167 A LBM BVW CG 2 CR Plecoptera (stoneflies) Perlidae 10 152 A LBM BVW PR 1 CR Perlodiae 1 15 A LBM BVW PR 2 CR Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 5 76 A LBM BVW SC 4 CG Elmidae 2 30 I LBM BVW SC 4 CG Diptera (true flies) (Culicidae) (3) - P LMB BVW ( ) Chironomidae 23 348 I LBM BVW CG 6 BU Simuliidae 13 197 I LBM BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
ii
jj
Other Total Individuals 66
(69) 1000
Total Taxa 7 (8)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Culicidae (Diptera) pupae not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl J Pangle S Robertson W Bousquet B Millholland J Tovar H Kepple
Collection date 30 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 1 12 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Asellidae 114 965 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Plecoptera (stoneflies) Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 2 23 A MRD BVW SC 4 CG Diptera (true flies)
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
kk
ll
Other Total Individuals 117 1000 Total Taxa 3
Notes Subsample taken 29 October 2009 All taxonomic determinations by MRD were confirmed by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson K McCann A Fisher H Kepple A Barrett
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 2 17 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Sphaeriidae 2 17 I MRD BVW CF 8 BU Isopoda (isopods) Asellidae 99 853 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Calopterygidae 2 17 A MRD BVW PR 7 CL Ephemeroptera (mayflies) Leptophlebiidae 2 17 I MRD BVW CG 2 CR Heptageniidae 4 34 A MRD BVW SC 3 CG Plecoptera (stoneflies) Perlidae 1 09 A MRD BVW PR 1 CR Trichoptera (caddisflies) Hemiptera (true bugs) (Gerridae) (1) - A MRD BVW ( ) Corixidae 1 09 A MRD BVW PR 5 CR Megaloptera (dobsonflies etc) Coleoptera (beetles) Dryopidae 1 09 A MRD BVW SC 5 CG Diptera (true flies) Tipulidae 2 17 I BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
mm
nn
Other Total Individuals 116
(117) 999
Total Taxa 10 (11)
Notes Subsample taken 6 November 2008 Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009 Gerridae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
- Disclaimer ii
- Title Page iii
- Acknowledgements v
- Table of Contents vii
- SECTION 2 RESULTS DATA TABLES 7
- OVERVIEW AND FINDINGS
- Introduction
- RESULTS DATA TABLES
- REFERENCES
- FIELD DATA SHEETS
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- SubstrateType
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- WATER QUALITY ANALYSIS
- THROUGH THE VIRGINIA
- STREAM CONDITION INDEX (VSCI)
- LABORATORY BENCH SHEETS
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
US Environmental Protection Agency 1997 Volunteer stream monitoring a methods manual EPA 841-B-97-003 Washington DC US Environmental Protection Agency Office of Water Van Meter Val 2003 Opequon Creek pollution limits to be established The Winchester Star 4 April B1 Virginia Department of Environmental Quality 2006a Virginia 305(b)303(d) water quality integrated report to Congress and the EPA Administrator for the period January 1 2000 to December 31 2004 Richmond Virginia Virginia Department of Environmental Quality Available online at httpwwwdeqvirginiagovwqair2006html __________ 2006b Using probabilistic data to validate the non-coastal Virginia Stream Condition Index (draft) VDEQ technical bulletin WQA2006-001 Virginia Department of Environmental Quality Available online at httpwwwloudounwatershedorgstream_assessmentreference_documents VA_DEQ_SCI_Validationpdf __________ 2008 Biological monitoring program quality assurance project plan for wadeable streams and rivers Richmond Virginia Virginia Department of Environmental Quality Update available online at httpwwwdeqstatevausbiomonitoringdocumentsBioMonQAPP_13Aug2008pdf Virginia Tech Aquatic Entomology Laboratory 2005 Taxa dictionary [Excel file] Blacksburg Virginia Virginia Tech Department of Biological Systems Engineering 2003a Opequon watershed TMDLs for benthic impairments Abrams Creek and Lower Opequon Creek Frederick and Clarke Counties Virginia Prepared for the Virginia Department of Environmental Quality and Virginia Department of Conservation and Recreation Blacksburg Virginia Available online at httpwwwdeqstate vaustmdlapptmdlsshenrvrabropebcpdf Voshell J Reese Jr 2002 A guide to common freshwater invertebrates of North America Blacksburg Virginia The McDonald amp Woodward Publishing Company
14
APPENDICES
15
16
a
Appendix A
FIELD DATA SHEETS
This appendix presents a Site Characterization Field Data Sheet for each of the six sampling reaches established on Hiatt Run and Lick Run for this study They were filled out during the sampling runs made in May 2008
These forms were adapted by Shenandoah University from forms used by the EPA and Virginia DEQ for recording characteristics of sampling sites
b
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Mouth STATION _HLR-1_____ STREAM CLASS LAT N 39 12749 (upstream) LONG W 078 04209(upstream) LAT N 39 12729 (downstream) LONG W 078 04148 (downstream) FORM COMPILED BY Jeremy Tovar RIVER BASIN Potomac River INVESTIGATORS JPangle B Millholland MDahl SRobertson W Bousquet JTovar BBousquet
DATE May 21 2008_________ TIME_250 PM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature __24___degC showers (intermittent) Comments _______________________
___ cloud cover _0_ ______________________________
X clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x ( Significant springs andor seeps) x (Valley ) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS
c
d
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence x FieldPasture Industrial Obvious sources Agricultural Other ____________ Stream scour steep slope erosion x Residential Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left Field and young thin deciduous forest 100 Bank=Grass cobble and shrub Right 90 maturing deciduous forest 45 shale slope Bank=Bedrock soil and herbs Indicate the dominant vegetation type(s) and record the dominant species
x Trees Shrubs x (Grasses) Forbs Dominant species Oak species and mix deciduous
INSTREAM FEATURES
Measured reach length ___100__m Canopy Cover Estimated stream width _35__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area __350_m2
Estimated stream depth _008_m Proportion of Reach Represented by Stream High water mark _15_m Morphology Types Surface velocity (at thalweg) _05__msec x Riffle__75___ x Run__20___ x Pool__5___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___00__ m2 Density of LWD _-_ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __15_____ x Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present Algae
WATER QUALITY Temperature __19___degC Water Odors x NormalNone Sewage Specific Conductance - Petroleum Chemical Fishy Other___________________ Dissolved Oxygen - Water Surface Oils Slick Sheen Globs Flecks pH __-_ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used _____NA________ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic x None Relict shells Other______None_____ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 8 Detritus sticks wood coarse plant materials
0 Boulder gt256 mm (10rsquorsquo) 0
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 51 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 40 Sand 006-2 mm (gritty) 1 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University
SITE CHARACTERIZATION FIELD DATA SHEET FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Package Plant STATION HLR-2 STREAM CLASS LAT N 39 129580 (upstream) LONG W 078 04900 (upstream) LAT N 39 129581 (downstream) LONG W 078 04822 (downstream) FORM COMPILED BY JW Pangle RIVER BASIN Potomac River INVESTIGATORS JPangle JTovar MDahl B Millholland SRobertson W Bousquet
DATE__19 May 2008_ TIME__1240__ PM
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature _____degC showers (intermittent) Comments __Sunny Day_____________
_5__ cloud cover _0__ ______________________________
x clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x (Significant springs andor seeps) x (Valley) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS
e
f
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence FieldPasture Industrial Obvious sources Agricultural Other ____________ __Upstream Construction and Stream bank Scour Residential __________________________________ Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left Deciduous forest with thick herb and shrub layers bank= vegetation and rocks Right Deciduous forest with thick herb and shrub layers bank=vegetation soil and rocks Indicate the dominant vegetation type(s) and record the dominant species
x Trees x Shrubs Grasses Forbs Dominant species Black Walnut White Ash Hackberry and American Sycamore
INSTREAM FEATURES
Measured reach length 100 m Canopy Cover Estimated stream width _35__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area __350_m2
Estimated stream depth __15__m Proportion of Reach Represented by Stream High water mark __22___m Morphology Types Surface velocity (at thalweg) _035__msec x Riffle__30___ x Run__30___ x Pool__40___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___10____ m2 Density of LWD _______ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __30___ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present__________________________________________________________
WATER QUALITY Temperature __18___degC Water Odors x NormalNone Sewage Specific Conductance __-____ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ____-____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used _____NA____ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other___None_____ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach SubstrateType Characteristic
Composition in Sampling Reach
Bedrock 3 Detritus sticks wood coarse plant materials
2 Boulder gt256 mm (10rsquorsquo) 5
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 70 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 22 Sand 006-2 mm (gritty) 0 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiattrsquos Run LOCATION Rt 664 Bridge STATION HLR-3 STREAM CLASS LAT N 39 12934 (upstream) LONG W 078 05019 (upstream) LAT N 39 12958 (downstream) LONG W 078 04958 (downstream) FORM COMPILED BY Sean Robertson RIVER BASIN Potomac River INVESTIGATORS SRobertson BMillholland WBousquet MDahl JPangle
DATE 28 May 2008 TIME 1127 AM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality 2008
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature __14___degC showers (intermittent) x Trace Comments _______________________
_65 x cloud cover ___ ______________________________
x clearsunny __________________________________
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x (Significant springs andor seeps) x (Valley) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS
g
h
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence Some evidence x FieldPasture Industrial x Obvious sources Agricultural x Other Old Package Plant Downstream x Residential Bridge and Road Power line and Pump station Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left 70 field 30 shrubs and deciduous trees Bank=Soil and vegetation Right Maturing deciduous forest steep slope Bank=Shale Bedrock Indicate the dominant vegetation type(s) and record the dominant species
x Trees x Shrubs x Grasses Forbs Dominant species Sycamore and Red Oaks
INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width __51___m Fully open x Partly open Partly shaded Fully shaded Sampling reach area _510_m2
Estimated stream depth __135___m Proportion of Reach Represented by Stream High water mark __-___m Morphology Types Surface velocity (at thalweg) __20___msec x Riffle_40_ x Run_50_ x Pool 10 Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___0____ m2 Density of LWD ___-____ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation ___50____ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present Brown Algae
WATER QUALITY Temperature __17___degC Water Odors x NormalNone Sewage Specific Conductance ___-___ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ___-_____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used ____NA_______ Clear x Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other____None____ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 15 Detritus sticks wood coarse plant materials
0 Boulder gt256 mm (10rsquorsquo) 15
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 62 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 8 Sand 006-2 mm (gritty) 0 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Unnamed Tributary STATION HLR-4 STREAM CLASS LAT N 39 13035 (upstream) LONG W 078 05129 (upstream) LAT N 39 13000 (downstream) LONG W 078 05086 (downstream) FORM COMPILED BY Marie Dahl RIVER BASIN Potomac River INVESTIGATORS M Dahl HKepple SRobertson AFisher
DATE 31 May 2008 TIME 5 PM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality 2008
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) YES x NO
rain (steady rain) Air Temperature __22___degC showers (intermittent) Comments _______________________
_0__ cloud cover _0__ ______________________________
x clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area __-___ km2 mi2 Montane Significant springs andor seeps x Valley Marsh or swamp Montane amp Valley Mixture of origins Other ___________________
COMMENTS Snowden Bridge development (upstream) and sewer line
i
j
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence x FieldPasture Industrial Obvious sources Agricultural Other ____________ Construction upstream and adjacent x Residential Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left Forested with rocky banks field across silt fence and 2m tree strip Right Forested with some rocky banks and forest through the highway Indicate the dominant vegetation type(s) and record the dominant species
x Trees x Shrubs x Grasses Forbs Dominant species White Ash and Hackberry
INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width ___38__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area __380__m2
Estimated stream depth __12__cm Proportion of Reach Represented by Stream High water mark __2___m Morphology Types Surface velocity (at thalweg) __46___msec x Riffle__25___ x Run__70___ x Pool__5___ Channelized Yes x No Dam Present x Yes No
LARGE WOODY DEBRIS
LWD _______ m2 Density of LWD _______ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation ___NA____ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present___Algae___
WATER QUALITY Temperature __16___degC Water Odors x NormalNone Sewage Specific Conductance __-____ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ___-_____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used ____NA_____ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells x Other___Shale_______ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 0 Detritus sticks wood coarse plant materials
10 Boulder gt256 mm (10rsquorsquo) 5
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 45 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 30 Sand 006-2 mm (gritty) 0 Marl grey shell
fragments 0
Silt 0004-006 mm 20 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Lick Run STATION HLR-5 STREAM CLASS LAT N 39 127517 (upstream) LONG W 078 05685(upstream) LAT N 39 12784 (downstream) LONG W 078 05631 (downstream) FORM COMPILED BY B Millholland RIVER BASIN Potomac River INVESTIGATORS B Millholland JTovar SRobertson MDahl WBousquet JPangle
DATE 29 May 2008 TIME 1040 AM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project
k
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature __15___degC showers (intermittent) Comments _______________________
_5_ X cloud cover _0__ ______________________________
x clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane Significant springs andor seeps x Valley Marsh or swamp Montane amp Valley Mixture of origins Other ___________________
COMMENTS Lick Run appears to be intermittent and may be dry in Fall 2008
l
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence x FieldPasture Industrial Obvious sources Agricultural Other ____________ Adjacent logging stream bank scour Residential Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left 85 young deciduous forest 15 logged Bank=Soil vegetation and cobbles Right Young deciduous forest on 30 slope Bank= Soil vegetation and cobble Indicate the dominant vegetation type(s) and record the dominant species
x Trees Shrubs Grasses Forbs Dominant species Mixed Deciduous INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width __11__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area 110 m2
Estimated stream depth _009__m Proportion of Reach Represented by Stream High water mark __96___m Morphology Types Surface velocity (at thalweg) __01___msec x Riffle__30___ x Run__5___ x Pool__65___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___0____ m2 Density of LWD ___-____ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __70___ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present__________________________________________________________
WATER QUALITY Temperature __14___degC Water Odors x NormalNone Sewage Specific Conductance __-__ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ___-___ Water Surface Oils Slick Sheen Globs Flecks pH __-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used _____NA______ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other_____None_____
SEDIMENT AND SUBSTRATE
Odors Deposits Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other________________ x Other Slightly Salty Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 0 Detritus sticks wood coarse plant materials
5 Boulder gt256 mm (10rsquorsquo) 0
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 75 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 23 Sand 006-2 mm (gritty) 2 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION McCann Farm STATION HLR-6 STREAM CLASS LAT N 39 13076 (upstream) LONG W 078 06539 (upstream) LAT N 39 13063 (downstream) LONG W 078 06473 (downstream) FORM COMPILED BY MDahl RIVER BASIN Potomac River INVESTIGATORS MDahl JPangle SRobertson W Bousqet BMillholland JTovar H Kepple
DATE 30 May 2008 TIME 1050 AM (start)
PROJECT NAME
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) YES x NO
rain (steady rain) Air Temperature __18___degC showers (intermittent) Comments _______________________
_85_x cloud cover _10__ ______________________________
clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x (Significant springs andor seeps) x ( Valley) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS Multi-habitat sampling with D-frame nets and seine
m
n
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial x No evidence Some evidence x FieldPasture Industrial Obvious sources x Agricultural Other ____________ Minor stream bank scour Residential Local Watershed Erosion x None Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left 35 bank grasses scattered trees and some bushes Right High bank with grasses and scattered trees Indicate the dominant vegetation type(s) and record the dominant species
x Trees Shrubs Grasses Forbs Dominant species Black Walnut and Festuca elatior
INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width __2___m Fully open Partly open x Partly shaded Fully shaded Sampling reach area __200_m2
Estimated stream depth __03_m Proportion of Reach Represented by Stream High water mark __1___m Morphology Types Surface velocity (at thalweg) __166_msec x Riffle__0___ x Run__55___ x Pool__45___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___2____ m2 Density of LWD ___-____ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __20_____ x Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present ____Glyceria (grass)sp__________
WATER QUALITY Temperature __16___degC Water Odors x NormalNone Sewage Specific Conductance ___-___ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ____-____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used ______NA___ Clear x Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other__NONE__ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 0 Detritus sticks wood coarse plant materials
5 Boulder gt256 mm (10rsquorsquo) 0
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 27 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 38 Sand 006-2 mm (gritty) 5 Marl grey shell
fragments 0 (lt1)
Silt 0004-006 mm 25 Clay lt 0004 mm (slick) 5 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Appendix B
WATER QUALITY ANALYSIS THROUGH THE VIRGINIA
STREAM CONDITION INDEX (VSCI)
The Virginia Stream Condition Index (VSCI) relies on eight metrics to categorize stream health According to Burton and Gerritsen (2003 p 1-1) this index ldquohellip can be used as a primary indicator of ecosystem health and can identify impairment with respect to the reference (or natural) conditionrdquo These metrics are numeric measures of a biological communityrsquos species composition diversity ecological niches or tolerance to pollution
Table 13 on the next page describes each VSCI metric lists its formula and provides the best standard value (BSV) for that metric Based on a 100-point scale the BSV is the score that reflects optimum conditions for aquatic life ie the best water quality value possible (DEQ 2006b pp 8 33) The table also explains whether the score increases or decreases with environmental stress
As Table 13 indicates each of the eight VSCI metrics is calculated individually Each formula provides a unitless score based on a 100-point scale with 0 as the worst possible score and 100 as the best Any metric that provides a result above 100 is changed to 100 in order for the index to work properly After the eight metrics are calculated they are summed and averaged to provide a final VSCI score on a 100-point scale
Based on statistical analysis of Virginiarsquos streams and their BMI communities the DEQ (2006b p 8) currently uses the following tiers to interpret VSCI scores
VSCI Scores Aquatic Life Use
(ALI) Tiers
lt 42 severely stressed
42-55 moderately stressed
55-63 unknownfair (gray zone)
63-73 good
gt73 excellent
Section 2 of this report presents the results of calculations for the six BMI sampling sites the research team studied in the Hiatt-Lick Run subwatershed in 2008
o
p
1 Total taxa the number of distinct taxa in the sample measures the overall diversity of the macroinvertebrate assemblage The best standard value (BSV) is 189 Score = 100 x (X189) Total taxa decreases with stress 2 EPT taxa the sum of the distinct taxa in the orders Ephemeroptera (mayflies) Plecoptera (stoneflies) and Trichoptera (caddisflies) These orders are known to be sensitive to pollution The best standard value (BSV) is 117 Score = 100 x (X117) EPT taxa decreases with stress 3 Percent Ephemeroptera the percent abundance of individuals in the sample that belong to the order Ephemeroptera (mayflies) The best standard value (BSV) is 477 Score = 100 x (X477) Percent Ephemeroptera decreases with stress 4 Percent Plecoptera + Trichoptera less Hydropsychidae the percent abundance of individuals in the sample that belong to the orders Plecoptera (stoneflies) and Trichoptera (caddisflies) excluding the pollution-tolerant caddisfly family Hydropsychidae The best standard value (BSV) is 562 Score = 100 x (X562) Percent Plecoptera + Trichoptera less Hydropsychidae decreases with stress 5 Percent Scrapers the percent abundance of individuals in the sample whose primary functional mechanism for feeding is grazing on the substrate or periphyton (ie attached algae and associated material) The best standard value (BSV) is 467 Score = 100 x (X467) Percent scrapers decreases with stress 6 Percent Chironomidae the percent abundance of individuals in the sample that are Chironomidae (non-biting midge) larvae and pupae The best standard value (BSV) is 16 Score = 100 x [(100-X)(100-16)] Percent Chironomidae increases with stress 7 Percent Top Two Dominant the percent abundance of the two most frequent taxa present in the sample The best standard value (BSV) is 328 Score = 100 x [(100-X)(100-328)] The Percent Top 2 Dominant increases with stress 8 Hilsenhoff Biotic Index (HBI) an abundance-weighted average pollution tolerance of all the individuals within a sample keyed to the family level Tolerance ratings range from 1 (highly intolerant) to 10 (highly tolerant) The best standard value (BSV) is 29 Score = 100 x [(10-X)(10-29)] The Hilsenhoff Biotic Index (HBI) increases with stress ______________________________________________________________________________ Table 13 Description of Benthic Macroinvertebrate VSCI Metrics Includes responses (increases decreases) of each VSCI metric to degraded water quality conditions Adapted from Burton and Gerritsen (2003 pp 3-15 3-16 6-2) and DEQ (2008)
q
Appendix C
LABORATORY BENCH SHEETS
Shenandoah Universityrsquos Environmental Studies Department developed the Benthic Macroinvertebrate Laboratory Bench Sheet and VSCI Worksheet to record BMI specimen identifications and their VSCI classifications for each sample
This form is based in part on bench sheets developed by the EPA and Virginia DEQ for recording specimen identifications Additional columns on Shenandoah Universityrsquos forms for relative numbers and VSCI classifications make the calculation and verification of VSCI scores easier
Note the column for taxonomistrsquos initials Specimens were identified by students in Shenandoah Universityrsquos research group and then verified or where necessary changed by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality This external review helps assure the validity of the study Originals of these bench sheets with handwritten taxonomistsrsquo initials will be kept on file at Shenandoah Universityrsquos Environmental Studies Department for five years after the completion of this study
r
s
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-1 STATION NAME Mouth LAT N 39deg 12749rsquo (upstream) LONG W 078deg 04209rsquo (upstream) LAT N 39deg 12729rsquo (downstream) LONG W 078deg 04148rsquo (downstream) FORM COMPILED BY Jeremy Tovar W S Bousquet RIVER BASIN Potomac River INVESTIGATORS Marie Dahl Brandon Millholand J W Pangle Sean Robertson Jeremy Tovar Woodward Bousquet Ben Bousquet
Collection date 21 May 2008 Sheet completion date 12 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Hirudinea 3 33 JT BVW PR 8 SP Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Coenagrionidae 2 22 BVW PR 9 CR Ephemeroptera (mayflies) Caenidae 1 11 JT BVW CG 6 SP Ephemerllidae 4 44 JT BVW CG 2 CR Heptageniidae 5 55 JT BVW SC 3 CG Isonychiidae 1 11 BVW CF 3 CG Plecoptera (stoneflies) Perlidae 14 154 JT BVW PR 2 CR Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Corydalidae 1 11 JT BVW PR 5 CR Coleoptera (beetles) Elmidae 24 264 I JT BVW SC 4 CG Dytiscidae 4 44 JT BVW PR 6 GN Psephenidae 3 33 JT BVW SC 4 CG Diptera (true flies) Tipulidae 3 33 JT BVW SH 4 BU Chironomidae 26 286 JT BVW CG 6 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
t
Other Total Individuals 91 1001 Total Taxa 13
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
u
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-1 STATION NAME Mouth LAT N 39deg 12749rsquo (upstream) LONG W 078deg 04209rsquo (upstream) LAT N 39deg 12729rsquo (downstream) LONG W 078deg 04148rsquo (downstream) FORM COMPILED BY Jeremy Tovar W S Bousquet RIVER BASIN Potomac River INVESTIGATORS Brandon Millholand JW Pangle Jeremy Tovar Heather Kepple
Collection date 10 October 2008 Sheet completion date 12 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Oligochaeta 3 35 JT BVW CG 8 BU Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Coenagrionidae 2 23 JT BVW PR 9 CR Ephemeroptera (mayflies) Heptageniidae 12 140 JT BVW SC 3 CG Isonychiidae 2 23 BVW CF 3 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Hydropsychidae 16 186 JT BVW CF 6 CG Philopotamidae 31 360 JT BVW CF 2 CG Hemiptera (true bugs) (Veliidae) (5) - JT BVW ( ) Megaloptera (dobsonflies etc) Corydalidae 10 116 JT BVW PR 5 CR Coleoptera (beetles) Dytiscidae 1 12 JT BVW PR 6 GN Psephenidae 7 81 JT BVW SC 4 CG Diptera (true flies) Tipulidae 2 23 BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
v
Other Total Individuals 86
(91) 999
Total Taxa 10 (11)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009 Veliidae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
w
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-2 STATION NAME Package Plant LAT N 39deg 12958rsquo (upstream) LONG W 078deg 04900 (upstream) LAT N 39deg 12958rsquo (downstream) LONG W 078deg 04822 (downstream) FORM COMPILED BY JW Pangle W Bousquet RIVER BASIN Potomac River INVESTIGATORS JW Pangle S Robertson J Tovar M Dahl B Millholland W Bousquet
Collection date 29 May 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Plecoptera (stoneflies) Perlidae 11 136 I JP BVW PR 1 CR Trichoptera (caddisflies) Hydropsychidae 10 123 I JP BVW CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 14 173 I JP BVW SC 4 CG Diptera (true flies) Chironomidae 32 395 I JP BVW CG 6 BU Simuliidae 14 173 I JP BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
x
Other Total Individuals 81 1000 Total Taxa 5
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-2 STATION NAME Package Plant LAT N 39deg 12958rsquo (upstream) LONG W 078deg 04900 (upstream) LAT N 39deg 12958rsquo (downstream) LONG W 078deg 04822 (downstream) FORM COMPILED BY JW Pangle W Bousquet RIVER BASIN Potomac River INVESTIGATORS JW Pangle Brandon Millholland Jeremy Tovar Heather Kepple
Collection date 10 October 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Heptageniidae 40 392 I WBJP SC 3 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Hydropsychidae 7 69 I JP WB BVW CF 6 CG Philopotamidae 28 275 I JP WB BVW CF 2 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Corydalidae 6 59 I JP WB BVW PR 5 CR Coleoptera (beetles) Psephenidae 7 69 I JP WB BVW SC 4 CG Elmidae 8 78 I JP WB BVW SC 4 CG Diptera (true flies) Tipulidae 6 59 I JP WB BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
y
z
Other Total Individuals 102 1001 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-3 STATION NAME Rt 664 Bridge LAT N 39deg 12934rsquo (upstream) LONG W 078deg 05019rsquo (upstream) LAT N 39deg 12951rsquo (downstream) LONG W 078deg 04958rsquo (downstream) FORM COMPILED BY S Robertson W Bousquet RIVER BASIN Potomac River INVESTIGATORS SRobertson BMillholland WBousquet MDahl JPangle
Collection date 28 May 2008 Sheet completion date 11 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Leptophlebiidae 1 15 I SGR BVW CG 2 CR Baetidae 1 15 I BVW CG 5 CG Plecoptera (stoneflies) Perlidae 3 46 I SGR BVW PR 1 CR Nemouridae 1 15 I BVW SH 3 CR Trichoptera (caddisflies) Hydropsychidae 5 77 I SGR BVW CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 27 415 I SGR BVW SC 4 CG Psephenidae 2 31 A SGR BVW SC 4 CG Diptera (true flies) Simuliidae 5 77 I SGR BVW CF 6 CG Chironomidae 20 308 I SGR BVW CG 6 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
aa
bb
Other Total Individuals 65 999 Total Taxa 9
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-3 STATION NAME Rt 664 Bridge LAT N 39deg 12934rsquo (upstream) LONG W 078deg 05019rsquo (upstream) LAT N 39deg 12951rsquo (downstream) LONG W 078deg 04958rsquo (downstream) FORM COMPILED BY S Robertson RIVER BASIN Potomac River INVESTIGATORS S Robertson M Dahl A Fisher H Kepple
Collection date 10 Oct 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Heptageniidae 2 20 I SGR SC 3 CG Isonychiidae 2 20 I BVW CF 2 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Philopotamidae 36 367 I SGR CF 2 CG Hydropsychidae 47 480 I SGR CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 2 20 A SGR SC 4 CG Psephenidae 8 82 A SGR SC 4 CG Diptera (true flies) Tabanidae 1 10 I SGR PR 7 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
cc
dd
Other Total Individuals 98 999 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-4 STATION NAME Unnamed Tributary LAT (upstream) N 39deg 13035rsquo LONG (upstream) W 078deg 05129rsquo LAT (downstream) N 39deg 13000rsquo LONG (downstream) W 078deg 5086rsquo FORM COMPILED BY A Fisher M Dahl rev W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson A Fisher H Kepple
Collection date 31 May 2008 Sheet completion date rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Flatworms (Turbellaria) Worms (Oligochaeta) Naididae 1 14 - BVW CG 8 BU Leeches (Hirudinea) Snails (Gastropoda) Clams (Bivalvia) Isopods (Isopoda) Asellidae 3 42 A AF BVW CG 8 SP Scuds (Amphipoda) Crayfish Shrimp (Decapoda) Water Mites (Hydracarina) Dragonflies (Odonata Anisoptera) Damselflies (Odonata Zygoptera) Mayflies (Ephemeroptera) Stoneflies (Plecoptera) Perlidae 2 28 I AF BVW PR 1 CR Caddisflies (Trichoptera) True Bugs (Hemiptera) Corixidae 1 14 I AF BVW MP 9 DV Dobsonflies (Megaloptera) Beetles (Coleoptera) Dytiscidae 1 14 I AF BVW PR 6 GN True Flies (Diptera) Chironomidae 61 847 I AF BVW CG 6 BU Simuliidae 3 42 I AF BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
ee
ff
Other Total Individuals 72 1001 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-4 STATION NAME Unnamed Tributary LAT (upstream) N 39deg 13035rsquo LONG (upstream) W 078deg 05129rsquo LAT (downstream) N 39deg 13000rsquo LONG (downstream) W 078deg 5086rsquo FORM COMPILED BY Andrew G M Fisher W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson A Fisher H Kepple
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Flatworms (Turbellaria) Worms (Oligochaeta) Lumbriculidae 2 32 A AF BVW CG 8 BU Leeches (Hirudinea) Snails (Gastropoda) Lymnaeidae 1 16 A BVW CG 9 GN Clams (Bivalvia) Isopods (Isopoda) Scuds (Amphipoda) Crayfish Shrimp (Decapoda) Cambaridae 1 16 A AF BVW GN 5 GN Water Mites (Hydracarina) Dragonflies (Odonata Anisoptera) Damselflies (Odonata Zygoptera) Calopterygidae 4 63 I BVW PR 7 CL Mayflies (Ephemeroptera) Tricorythidae 1 16 I BVW CG 5 SP Stoneflies (Plecoptera) Caddisflies (Trichoptera) Phryganeidae 4 63 I AF BVW SH 4 CR Hydropsychidae 6 95 I AF BVW CF 6 CG I True Bugs (Hemiptera) Corixidae 8 127 I AF BVW MP 9 DV (Veliidae ) (7) - I AF BVW ( ) Dobsonflies (Megaloptera) Sialidae 3 48 I AF BVW PR 7 SP Corydalidae 2 32 I AF BVW PR 5 CR Beetles (Coleoptera) Haliplidae 1 16 A AF BVW MP 8 GN Dysticidae 7 111 I AF BVW PR 6 GN Hydrophilidae 1 16 A AF BVW PR 8 GN Elmidae 3 48 I BVW SC 4 CG True Flies (Diptera) Chironomidae 8 127 I AF BVW CG 6 BU Simuliidae 7 111 I AF BVW CF 6 CG Tipulidae 4 63 I AF BVW SH 4 GU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
gg
hh
Other Total Individuals 63
(70) 1000
Total Taxa 17 (18)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Veliidae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-5 STATION NAME Lick Run LAT N 39deg 12752rsquo (upstream) LONG W 078deg 05685rsquo (upstream) LAT N 39deg 12784rsquo (downstream) LONG W 078deg 05631rsquo (downstream) FORM COMPILED BY Brandon Millholland W Bousquet RIVER BASIN Potomac River INVESTIGATORS J Pangle S Robertson W Bousquet JTovar M Dahl
Collection date 29 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Gomphidae 1 15 I BVW PR 3 BU Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Leptophlebiidae 11 167 A LBM BVW CG 2 CR Plecoptera (stoneflies) Perlidae 10 152 A LBM BVW PR 1 CR Perlodiae 1 15 A LBM BVW PR 2 CR Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 5 76 A LBM BVW SC 4 CG Elmidae 2 30 I LBM BVW SC 4 CG Diptera (true flies) (Culicidae) (3) - P LMB BVW ( ) Chironomidae 23 348 I LBM BVW CG 6 BU Simuliidae 13 197 I LBM BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
ii
jj
Other Total Individuals 66
(69) 1000
Total Taxa 7 (8)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Culicidae (Diptera) pupae not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl J Pangle S Robertson W Bousquet B Millholland J Tovar H Kepple
Collection date 30 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 1 12 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Asellidae 114 965 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Plecoptera (stoneflies) Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 2 23 A MRD BVW SC 4 CG Diptera (true flies)
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
kk
ll
Other Total Individuals 117 1000 Total Taxa 3
Notes Subsample taken 29 October 2009 All taxonomic determinations by MRD were confirmed by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson K McCann A Fisher H Kepple A Barrett
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 2 17 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Sphaeriidae 2 17 I MRD BVW CF 8 BU Isopoda (isopods) Asellidae 99 853 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Calopterygidae 2 17 A MRD BVW PR 7 CL Ephemeroptera (mayflies) Leptophlebiidae 2 17 I MRD BVW CG 2 CR Heptageniidae 4 34 A MRD BVW SC 3 CG Plecoptera (stoneflies) Perlidae 1 09 A MRD BVW PR 1 CR Trichoptera (caddisflies) Hemiptera (true bugs) (Gerridae) (1) - A MRD BVW ( ) Corixidae 1 09 A MRD BVW PR 5 CR Megaloptera (dobsonflies etc) Coleoptera (beetles) Dryopidae 1 09 A MRD BVW SC 5 CG Diptera (true flies) Tipulidae 2 17 I BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
mm
nn
Other Total Individuals 116
(117) 999
Total Taxa 10 (11)
Notes Subsample taken 6 November 2008 Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009 Gerridae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
- Disclaimer ii
- Title Page iii
- Acknowledgements v
- Table of Contents vii
- SECTION 2 RESULTS DATA TABLES 7
- OVERVIEW AND FINDINGS
- Introduction
- RESULTS DATA TABLES
- REFERENCES
- FIELD DATA SHEETS
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- SubstrateType
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- WATER QUALITY ANALYSIS
- THROUGH THE VIRGINIA
- STREAM CONDITION INDEX (VSCI)
- LABORATORY BENCH SHEETS
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
APPENDICES
15
16
a
Appendix A
FIELD DATA SHEETS
This appendix presents a Site Characterization Field Data Sheet for each of the six sampling reaches established on Hiatt Run and Lick Run for this study They were filled out during the sampling runs made in May 2008
These forms were adapted by Shenandoah University from forms used by the EPA and Virginia DEQ for recording characteristics of sampling sites
b
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Mouth STATION _HLR-1_____ STREAM CLASS LAT N 39 12749 (upstream) LONG W 078 04209(upstream) LAT N 39 12729 (downstream) LONG W 078 04148 (downstream) FORM COMPILED BY Jeremy Tovar RIVER BASIN Potomac River INVESTIGATORS JPangle B Millholland MDahl SRobertson W Bousquet JTovar BBousquet
DATE May 21 2008_________ TIME_250 PM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature __24___degC showers (intermittent) Comments _______________________
___ cloud cover _0_ ______________________________
X clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x ( Significant springs andor seeps) x (Valley ) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS
c
d
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence x FieldPasture Industrial Obvious sources Agricultural Other ____________ Stream scour steep slope erosion x Residential Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left Field and young thin deciduous forest 100 Bank=Grass cobble and shrub Right 90 maturing deciduous forest 45 shale slope Bank=Bedrock soil and herbs Indicate the dominant vegetation type(s) and record the dominant species
x Trees Shrubs x (Grasses) Forbs Dominant species Oak species and mix deciduous
INSTREAM FEATURES
Measured reach length ___100__m Canopy Cover Estimated stream width _35__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area __350_m2
Estimated stream depth _008_m Proportion of Reach Represented by Stream High water mark _15_m Morphology Types Surface velocity (at thalweg) _05__msec x Riffle__75___ x Run__20___ x Pool__5___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___00__ m2 Density of LWD _-_ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __15_____ x Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present Algae
WATER QUALITY Temperature __19___degC Water Odors x NormalNone Sewage Specific Conductance - Petroleum Chemical Fishy Other___________________ Dissolved Oxygen - Water Surface Oils Slick Sheen Globs Flecks pH __-_ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used _____NA________ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic x None Relict shells Other______None_____ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 8 Detritus sticks wood coarse plant materials
0 Boulder gt256 mm (10rsquorsquo) 0
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 51 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 40 Sand 006-2 mm (gritty) 1 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University
SITE CHARACTERIZATION FIELD DATA SHEET FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Package Plant STATION HLR-2 STREAM CLASS LAT N 39 129580 (upstream) LONG W 078 04900 (upstream) LAT N 39 129581 (downstream) LONG W 078 04822 (downstream) FORM COMPILED BY JW Pangle RIVER BASIN Potomac River INVESTIGATORS JPangle JTovar MDahl B Millholland SRobertson W Bousquet
DATE__19 May 2008_ TIME__1240__ PM
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature _____degC showers (intermittent) Comments __Sunny Day_____________
_5__ cloud cover _0__ ______________________________
x clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x (Significant springs andor seeps) x (Valley) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS
e
f
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence FieldPasture Industrial Obvious sources Agricultural Other ____________ __Upstream Construction and Stream bank Scour Residential __________________________________ Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left Deciduous forest with thick herb and shrub layers bank= vegetation and rocks Right Deciduous forest with thick herb and shrub layers bank=vegetation soil and rocks Indicate the dominant vegetation type(s) and record the dominant species
x Trees x Shrubs Grasses Forbs Dominant species Black Walnut White Ash Hackberry and American Sycamore
INSTREAM FEATURES
Measured reach length 100 m Canopy Cover Estimated stream width _35__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area __350_m2
Estimated stream depth __15__m Proportion of Reach Represented by Stream High water mark __22___m Morphology Types Surface velocity (at thalweg) _035__msec x Riffle__30___ x Run__30___ x Pool__40___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___10____ m2 Density of LWD _______ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __30___ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present__________________________________________________________
WATER QUALITY Temperature __18___degC Water Odors x NormalNone Sewage Specific Conductance __-____ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ____-____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used _____NA____ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other___None_____ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach SubstrateType Characteristic
Composition in Sampling Reach
Bedrock 3 Detritus sticks wood coarse plant materials
2 Boulder gt256 mm (10rsquorsquo) 5
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 70 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 22 Sand 006-2 mm (gritty) 0 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiattrsquos Run LOCATION Rt 664 Bridge STATION HLR-3 STREAM CLASS LAT N 39 12934 (upstream) LONG W 078 05019 (upstream) LAT N 39 12958 (downstream) LONG W 078 04958 (downstream) FORM COMPILED BY Sean Robertson RIVER BASIN Potomac River INVESTIGATORS SRobertson BMillholland WBousquet MDahl JPangle
DATE 28 May 2008 TIME 1127 AM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality 2008
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature __14___degC showers (intermittent) x Trace Comments _______________________
_65 x cloud cover ___ ______________________________
x clearsunny __________________________________
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x (Significant springs andor seeps) x (Valley) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS
g
h
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence Some evidence x FieldPasture Industrial x Obvious sources Agricultural x Other Old Package Plant Downstream x Residential Bridge and Road Power line and Pump station Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left 70 field 30 shrubs and deciduous trees Bank=Soil and vegetation Right Maturing deciduous forest steep slope Bank=Shale Bedrock Indicate the dominant vegetation type(s) and record the dominant species
x Trees x Shrubs x Grasses Forbs Dominant species Sycamore and Red Oaks
INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width __51___m Fully open x Partly open Partly shaded Fully shaded Sampling reach area _510_m2
Estimated stream depth __135___m Proportion of Reach Represented by Stream High water mark __-___m Morphology Types Surface velocity (at thalweg) __20___msec x Riffle_40_ x Run_50_ x Pool 10 Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___0____ m2 Density of LWD ___-____ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation ___50____ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present Brown Algae
WATER QUALITY Temperature __17___degC Water Odors x NormalNone Sewage Specific Conductance ___-___ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ___-_____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used ____NA_______ Clear x Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other____None____ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 15 Detritus sticks wood coarse plant materials
0 Boulder gt256 mm (10rsquorsquo) 15
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 62 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 8 Sand 006-2 mm (gritty) 0 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Unnamed Tributary STATION HLR-4 STREAM CLASS LAT N 39 13035 (upstream) LONG W 078 05129 (upstream) LAT N 39 13000 (downstream) LONG W 078 05086 (downstream) FORM COMPILED BY Marie Dahl RIVER BASIN Potomac River INVESTIGATORS M Dahl HKepple SRobertson AFisher
DATE 31 May 2008 TIME 5 PM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality 2008
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) YES x NO
rain (steady rain) Air Temperature __22___degC showers (intermittent) Comments _______________________
_0__ cloud cover _0__ ______________________________
x clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area __-___ km2 mi2 Montane Significant springs andor seeps x Valley Marsh or swamp Montane amp Valley Mixture of origins Other ___________________
COMMENTS Snowden Bridge development (upstream) and sewer line
i
j
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence x FieldPasture Industrial Obvious sources Agricultural Other ____________ Construction upstream and adjacent x Residential Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left Forested with rocky banks field across silt fence and 2m tree strip Right Forested with some rocky banks and forest through the highway Indicate the dominant vegetation type(s) and record the dominant species
x Trees x Shrubs x Grasses Forbs Dominant species White Ash and Hackberry
INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width ___38__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area __380__m2
Estimated stream depth __12__cm Proportion of Reach Represented by Stream High water mark __2___m Morphology Types Surface velocity (at thalweg) __46___msec x Riffle__25___ x Run__70___ x Pool__5___ Channelized Yes x No Dam Present x Yes No
LARGE WOODY DEBRIS
LWD _______ m2 Density of LWD _______ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation ___NA____ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present___Algae___
WATER QUALITY Temperature __16___degC Water Odors x NormalNone Sewage Specific Conductance __-____ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ___-_____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used ____NA_____ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells x Other___Shale_______ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 0 Detritus sticks wood coarse plant materials
10 Boulder gt256 mm (10rsquorsquo) 5
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 45 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 30 Sand 006-2 mm (gritty) 0 Marl grey shell
fragments 0
Silt 0004-006 mm 20 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Lick Run STATION HLR-5 STREAM CLASS LAT N 39 127517 (upstream) LONG W 078 05685(upstream) LAT N 39 12784 (downstream) LONG W 078 05631 (downstream) FORM COMPILED BY B Millholland RIVER BASIN Potomac River INVESTIGATORS B Millholland JTovar SRobertson MDahl WBousquet JPangle
DATE 29 May 2008 TIME 1040 AM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project
k
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature __15___degC showers (intermittent) Comments _______________________
_5_ X cloud cover _0__ ______________________________
x clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane Significant springs andor seeps x Valley Marsh or swamp Montane amp Valley Mixture of origins Other ___________________
COMMENTS Lick Run appears to be intermittent and may be dry in Fall 2008
l
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence x FieldPasture Industrial Obvious sources Agricultural Other ____________ Adjacent logging stream bank scour Residential Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left 85 young deciduous forest 15 logged Bank=Soil vegetation and cobbles Right Young deciduous forest on 30 slope Bank= Soil vegetation and cobble Indicate the dominant vegetation type(s) and record the dominant species
x Trees Shrubs Grasses Forbs Dominant species Mixed Deciduous INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width __11__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area 110 m2
Estimated stream depth _009__m Proportion of Reach Represented by Stream High water mark __96___m Morphology Types Surface velocity (at thalweg) __01___msec x Riffle__30___ x Run__5___ x Pool__65___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___0____ m2 Density of LWD ___-____ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __70___ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present__________________________________________________________
WATER QUALITY Temperature __14___degC Water Odors x NormalNone Sewage Specific Conductance __-__ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ___-___ Water Surface Oils Slick Sheen Globs Flecks pH __-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used _____NA______ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other_____None_____
SEDIMENT AND SUBSTRATE
Odors Deposits Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other________________ x Other Slightly Salty Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 0 Detritus sticks wood coarse plant materials
5 Boulder gt256 mm (10rsquorsquo) 0
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 75 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 23 Sand 006-2 mm (gritty) 2 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION McCann Farm STATION HLR-6 STREAM CLASS LAT N 39 13076 (upstream) LONG W 078 06539 (upstream) LAT N 39 13063 (downstream) LONG W 078 06473 (downstream) FORM COMPILED BY MDahl RIVER BASIN Potomac River INVESTIGATORS MDahl JPangle SRobertson W Bousqet BMillholland JTovar H Kepple
DATE 30 May 2008 TIME 1050 AM (start)
PROJECT NAME
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) YES x NO
rain (steady rain) Air Temperature __18___degC showers (intermittent) Comments _______________________
_85_x cloud cover _10__ ______________________________
clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x (Significant springs andor seeps) x ( Valley) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS Multi-habitat sampling with D-frame nets and seine
m
n
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial x No evidence Some evidence x FieldPasture Industrial Obvious sources x Agricultural Other ____________ Minor stream bank scour Residential Local Watershed Erosion x None Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left 35 bank grasses scattered trees and some bushes Right High bank with grasses and scattered trees Indicate the dominant vegetation type(s) and record the dominant species
x Trees Shrubs Grasses Forbs Dominant species Black Walnut and Festuca elatior
INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width __2___m Fully open Partly open x Partly shaded Fully shaded Sampling reach area __200_m2
Estimated stream depth __03_m Proportion of Reach Represented by Stream High water mark __1___m Morphology Types Surface velocity (at thalweg) __166_msec x Riffle__0___ x Run__55___ x Pool__45___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___2____ m2 Density of LWD ___-____ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __20_____ x Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present ____Glyceria (grass)sp__________
WATER QUALITY Temperature __16___degC Water Odors x NormalNone Sewage Specific Conductance ___-___ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ____-____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used ______NA___ Clear x Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other__NONE__ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 0 Detritus sticks wood coarse plant materials
5 Boulder gt256 mm (10rsquorsquo) 0
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 27 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 38 Sand 006-2 mm (gritty) 5 Marl grey shell
fragments 0 (lt1)
Silt 0004-006 mm 25 Clay lt 0004 mm (slick) 5 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Appendix B
WATER QUALITY ANALYSIS THROUGH THE VIRGINIA
STREAM CONDITION INDEX (VSCI)
The Virginia Stream Condition Index (VSCI) relies on eight metrics to categorize stream health According to Burton and Gerritsen (2003 p 1-1) this index ldquohellip can be used as a primary indicator of ecosystem health and can identify impairment with respect to the reference (or natural) conditionrdquo These metrics are numeric measures of a biological communityrsquos species composition diversity ecological niches or tolerance to pollution
Table 13 on the next page describes each VSCI metric lists its formula and provides the best standard value (BSV) for that metric Based on a 100-point scale the BSV is the score that reflects optimum conditions for aquatic life ie the best water quality value possible (DEQ 2006b pp 8 33) The table also explains whether the score increases or decreases with environmental stress
As Table 13 indicates each of the eight VSCI metrics is calculated individually Each formula provides a unitless score based on a 100-point scale with 0 as the worst possible score and 100 as the best Any metric that provides a result above 100 is changed to 100 in order for the index to work properly After the eight metrics are calculated they are summed and averaged to provide a final VSCI score on a 100-point scale
Based on statistical analysis of Virginiarsquos streams and their BMI communities the DEQ (2006b p 8) currently uses the following tiers to interpret VSCI scores
VSCI Scores Aquatic Life Use
(ALI) Tiers
lt 42 severely stressed
42-55 moderately stressed
55-63 unknownfair (gray zone)
63-73 good
gt73 excellent
Section 2 of this report presents the results of calculations for the six BMI sampling sites the research team studied in the Hiatt-Lick Run subwatershed in 2008
o
p
1 Total taxa the number of distinct taxa in the sample measures the overall diversity of the macroinvertebrate assemblage The best standard value (BSV) is 189 Score = 100 x (X189) Total taxa decreases with stress 2 EPT taxa the sum of the distinct taxa in the orders Ephemeroptera (mayflies) Plecoptera (stoneflies) and Trichoptera (caddisflies) These orders are known to be sensitive to pollution The best standard value (BSV) is 117 Score = 100 x (X117) EPT taxa decreases with stress 3 Percent Ephemeroptera the percent abundance of individuals in the sample that belong to the order Ephemeroptera (mayflies) The best standard value (BSV) is 477 Score = 100 x (X477) Percent Ephemeroptera decreases with stress 4 Percent Plecoptera + Trichoptera less Hydropsychidae the percent abundance of individuals in the sample that belong to the orders Plecoptera (stoneflies) and Trichoptera (caddisflies) excluding the pollution-tolerant caddisfly family Hydropsychidae The best standard value (BSV) is 562 Score = 100 x (X562) Percent Plecoptera + Trichoptera less Hydropsychidae decreases with stress 5 Percent Scrapers the percent abundance of individuals in the sample whose primary functional mechanism for feeding is grazing on the substrate or periphyton (ie attached algae and associated material) The best standard value (BSV) is 467 Score = 100 x (X467) Percent scrapers decreases with stress 6 Percent Chironomidae the percent abundance of individuals in the sample that are Chironomidae (non-biting midge) larvae and pupae The best standard value (BSV) is 16 Score = 100 x [(100-X)(100-16)] Percent Chironomidae increases with stress 7 Percent Top Two Dominant the percent abundance of the two most frequent taxa present in the sample The best standard value (BSV) is 328 Score = 100 x [(100-X)(100-328)] The Percent Top 2 Dominant increases with stress 8 Hilsenhoff Biotic Index (HBI) an abundance-weighted average pollution tolerance of all the individuals within a sample keyed to the family level Tolerance ratings range from 1 (highly intolerant) to 10 (highly tolerant) The best standard value (BSV) is 29 Score = 100 x [(10-X)(10-29)] The Hilsenhoff Biotic Index (HBI) increases with stress ______________________________________________________________________________ Table 13 Description of Benthic Macroinvertebrate VSCI Metrics Includes responses (increases decreases) of each VSCI metric to degraded water quality conditions Adapted from Burton and Gerritsen (2003 pp 3-15 3-16 6-2) and DEQ (2008)
q
Appendix C
LABORATORY BENCH SHEETS
Shenandoah Universityrsquos Environmental Studies Department developed the Benthic Macroinvertebrate Laboratory Bench Sheet and VSCI Worksheet to record BMI specimen identifications and their VSCI classifications for each sample
This form is based in part on bench sheets developed by the EPA and Virginia DEQ for recording specimen identifications Additional columns on Shenandoah Universityrsquos forms for relative numbers and VSCI classifications make the calculation and verification of VSCI scores easier
Note the column for taxonomistrsquos initials Specimens were identified by students in Shenandoah Universityrsquos research group and then verified or where necessary changed by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality This external review helps assure the validity of the study Originals of these bench sheets with handwritten taxonomistsrsquo initials will be kept on file at Shenandoah Universityrsquos Environmental Studies Department for five years after the completion of this study
r
s
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-1 STATION NAME Mouth LAT N 39deg 12749rsquo (upstream) LONG W 078deg 04209rsquo (upstream) LAT N 39deg 12729rsquo (downstream) LONG W 078deg 04148rsquo (downstream) FORM COMPILED BY Jeremy Tovar W S Bousquet RIVER BASIN Potomac River INVESTIGATORS Marie Dahl Brandon Millholand J W Pangle Sean Robertson Jeremy Tovar Woodward Bousquet Ben Bousquet
Collection date 21 May 2008 Sheet completion date 12 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Hirudinea 3 33 JT BVW PR 8 SP Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Coenagrionidae 2 22 BVW PR 9 CR Ephemeroptera (mayflies) Caenidae 1 11 JT BVW CG 6 SP Ephemerllidae 4 44 JT BVW CG 2 CR Heptageniidae 5 55 JT BVW SC 3 CG Isonychiidae 1 11 BVW CF 3 CG Plecoptera (stoneflies) Perlidae 14 154 JT BVW PR 2 CR Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Corydalidae 1 11 JT BVW PR 5 CR Coleoptera (beetles) Elmidae 24 264 I JT BVW SC 4 CG Dytiscidae 4 44 JT BVW PR 6 GN Psephenidae 3 33 JT BVW SC 4 CG Diptera (true flies) Tipulidae 3 33 JT BVW SH 4 BU Chironomidae 26 286 JT BVW CG 6 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
t
Other Total Individuals 91 1001 Total Taxa 13
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
u
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-1 STATION NAME Mouth LAT N 39deg 12749rsquo (upstream) LONG W 078deg 04209rsquo (upstream) LAT N 39deg 12729rsquo (downstream) LONG W 078deg 04148rsquo (downstream) FORM COMPILED BY Jeremy Tovar W S Bousquet RIVER BASIN Potomac River INVESTIGATORS Brandon Millholand JW Pangle Jeremy Tovar Heather Kepple
Collection date 10 October 2008 Sheet completion date 12 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Oligochaeta 3 35 JT BVW CG 8 BU Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Coenagrionidae 2 23 JT BVW PR 9 CR Ephemeroptera (mayflies) Heptageniidae 12 140 JT BVW SC 3 CG Isonychiidae 2 23 BVW CF 3 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Hydropsychidae 16 186 JT BVW CF 6 CG Philopotamidae 31 360 JT BVW CF 2 CG Hemiptera (true bugs) (Veliidae) (5) - JT BVW ( ) Megaloptera (dobsonflies etc) Corydalidae 10 116 JT BVW PR 5 CR Coleoptera (beetles) Dytiscidae 1 12 JT BVW PR 6 GN Psephenidae 7 81 JT BVW SC 4 CG Diptera (true flies) Tipulidae 2 23 BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
v
Other Total Individuals 86
(91) 999
Total Taxa 10 (11)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009 Veliidae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
w
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-2 STATION NAME Package Plant LAT N 39deg 12958rsquo (upstream) LONG W 078deg 04900 (upstream) LAT N 39deg 12958rsquo (downstream) LONG W 078deg 04822 (downstream) FORM COMPILED BY JW Pangle W Bousquet RIVER BASIN Potomac River INVESTIGATORS JW Pangle S Robertson J Tovar M Dahl B Millholland W Bousquet
Collection date 29 May 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Plecoptera (stoneflies) Perlidae 11 136 I JP BVW PR 1 CR Trichoptera (caddisflies) Hydropsychidae 10 123 I JP BVW CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 14 173 I JP BVW SC 4 CG Diptera (true flies) Chironomidae 32 395 I JP BVW CG 6 BU Simuliidae 14 173 I JP BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
x
Other Total Individuals 81 1000 Total Taxa 5
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-2 STATION NAME Package Plant LAT N 39deg 12958rsquo (upstream) LONG W 078deg 04900 (upstream) LAT N 39deg 12958rsquo (downstream) LONG W 078deg 04822 (downstream) FORM COMPILED BY JW Pangle W Bousquet RIVER BASIN Potomac River INVESTIGATORS JW Pangle Brandon Millholland Jeremy Tovar Heather Kepple
Collection date 10 October 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Heptageniidae 40 392 I WBJP SC 3 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Hydropsychidae 7 69 I JP WB BVW CF 6 CG Philopotamidae 28 275 I JP WB BVW CF 2 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Corydalidae 6 59 I JP WB BVW PR 5 CR Coleoptera (beetles) Psephenidae 7 69 I JP WB BVW SC 4 CG Elmidae 8 78 I JP WB BVW SC 4 CG Diptera (true flies) Tipulidae 6 59 I JP WB BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
y
z
Other Total Individuals 102 1001 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-3 STATION NAME Rt 664 Bridge LAT N 39deg 12934rsquo (upstream) LONG W 078deg 05019rsquo (upstream) LAT N 39deg 12951rsquo (downstream) LONG W 078deg 04958rsquo (downstream) FORM COMPILED BY S Robertson W Bousquet RIVER BASIN Potomac River INVESTIGATORS SRobertson BMillholland WBousquet MDahl JPangle
Collection date 28 May 2008 Sheet completion date 11 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Leptophlebiidae 1 15 I SGR BVW CG 2 CR Baetidae 1 15 I BVW CG 5 CG Plecoptera (stoneflies) Perlidae 3 46 I SGR BVW PR 1 CR Nemouridae 1 15 I BVW SH 3 CR Trichoptera (caddisflies) Hydropsychidae 5 77 I SGR BVW CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 27 415 I SGR BVW SC 4 CG Psephenidae 2 31 A SGR BVW SC 4 CG Diptera (true flies) Simuliidae 5 77 I SGR BVW CF 6 CG Chironomidae 20 308 I SGR BVW CG 6 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
aa
bb
Other Total Individuals 65 999 Total Taxa 9
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-3 STATION NAME Rt 664 Bridge LAT N 39deg 12934rsquo (upstream) LONG W 078deg 05019rsquo (upstream) LAT N 39deg 12951rsquo (downstream) LONG W 078deg 04958rsquo (downstream) FORM COMPILED BY S Robertson RIVER BASIN Potomac River INVESTIGATORS S Robertson M Dahl A Fisher H Kepple
Collection date 10 Oct 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Heptageniidae 2 20 I SGR SC 3 CG Isonychiidae 2 20 I BVW CF 2 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Philopotamidae 36 367 I SGR CF 2 CG Hydropsychidae 47 480 I SGR CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 2 20 A SGR SC 4 CG Psephenidae 8 82 A SGR SC 4 CG Diptera (true flies) Tabanidae 1 10 I SGR PR 7 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
cc
dd
Other Total Individuals 98 999 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-4 STATION NAME Unnamed Tributary LAT (upstream) N 39deg 13035rsquo LONG (upstream) W 078deg 05129rsquo LAT (downstream) N 39deg 13000rsquo LONG (downstream) W 078deg 5086rsquo FORM COMPILED BY A Fisher M Dahl rev W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson A Fisher H Kepple
Collection date 31 May 2008 Sheet completion date rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Flatworms (Turbellaria) Worms (Oligochaeta) Naididae 1 14 - BVW CG 8 BU Leeches (Hirudinea) Snails (Gastropoda) Clams (Bivalvia) Isopods (Isopoda) Asellidae 3 42 A AF BVW CG 8 SP Scuds (Amphipoda) Crayfish Shrimp (Decapoda) Water Mites (Hydracarina) Dragonflies (Odonata Anisoptera) Damselflies (Odonata Zygoptera) Mayflies (Ephemeroptera) Stoneflies (Plecoptera) Perlidae 2 28 I AF BVW PR 1 CR Caddisflies (Trichoptera) True Bugs (Hemiptera) Corixidae 1 14 I AF BVW MP 9 DV Dobsonflies (Megaloptera) Beetles (Coleoptera) Dytiscidae 1 14 I AF BVW PR 6 GN True Flies (Diptera) Chironomidae 61 847 I AF BVW CG 6 BU Simuliidae 3 42 I AF BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
ee
ff
Other Total Individuals 72 1001 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-4 STATION NAME Unnamed Tributary LAT (upstream) N 39deg 13035rsquo LONG (upstream) W 078deg 05129rsquo LAT (downstream) N 39deg 13000rsquo LONG (downstream) W 078deg 5086rsquo FORM COMPILED BY Andrew G M Fisher W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson A Fisher H Kepple
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Flatworms (Turbellaria) Worms (Oligochaeta) Lumbriculidae 2 32 A AF BVW CG 8 BU Leeches (Hirudinea) Snails (Gastropoda) Lymnaeidae 1 16 A BVW CG 9 GN Clams (Bivalvia) Isopods (Isopoda) Scuds (Amphipoda) Crayfish Shrimp (Decapoda) Cambaridae 1 16 A AF BVW GN 5 GN Water Mites (Hydracarina) Dragonflies (Odonata Anisoptera) Damselflies (Odonata Zygoptera) Calopterygidae 4 63 I BVW PR 7 CL Mayflies (Ephemeroptera) Tricorythidae 1 16 I BVW CG 5 SP Stoneflies (Plecoptera) Caddisflies (Trichoptera) Phryganeidae 4 63 I AF BVW SH 4 CR Hydropsychidae 6 95 I AF BVW CF 6 CG I True Bugs (Hemiptera) Corixidae 8 127 I AF BVW MP 9 DV (Veliidae ) (7) - I AF BVW ( ) Dobsonflies (Megaloptera) Sialidae 3 48 I AF BVW PR 7 SP Corydalidae 2 32 I AF BVW PR 5 CR Beetles (Coleoptera) Haliplidae 1 16 A AF BVW MP 8 GN Dysticidae 7 111 I AF BVW PR 6 GN Hydrophilidae 1 16 A AF BVW PR 8 GN Elmidae 3 48 I BVW SC 4 CG True Flies (Diptera) Chironomidae 8 127 I AF BVW CG 6 BU Simuliidae 7 111 I AF BVW CF 6 CG Tipulidae 4 63 I AF BVW SH 4 GU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
gg
hh
Other Total Individuals 63
(70) 1000
Total Taxa 17 (18)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Veliidae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-5 STATION NAME Lick Run LAT N 39deg 12752rsquo (upstream) LONG W 078deg 05685rsquo (upstream) LAT N 39deg 12784rsquo (downstream) LONG W 078deg 05631rsquo (downstream) FORM COMPILED BY Brandon Millholland W Bousquet RIVER BASIN Potomac River INVESTIGATORS J Pangle S Robertson W Bousquet JTovar M Dahl
Collection date 29 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Gomphidae 1 15 I BVW PR 3 BU Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Leptophlebiidae 11 167 A LBM BVW CG 2 CR Plecoptera (stoneflies) Perlidae 10 152 A LBM BVW PR 1 CR Perlodiae 1 15 A LBM BVW PR 2 CR Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 5 76 A LBM BVW SC 4 CG Elmidae 2 30 I LBM BVW SC 4 CG Diptera (true flies) (Culicidae) (3) - P LMB BVW ( ) Chironomidae 23 348 I LBM BVW CG 6 BU Simuliidae 13 197 I LBM BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
ii
jj
Other Total Individuals 66
(69) 1000
Total Taxa 7 (8)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Culicidae (Diptera) pupae not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl J Pangle S Robertson W Bousquet B Millholland J Tovar H Kepple
Collection date 30 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 1 12 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Asellidae 114 965 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Plecoptera (stoneflies) Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 2 23 A MRD BVW SC 4 CG Diptera (true flies)
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
kk
ll
Other Total Individuals 117 1000 Total Taxa 3
Notes Subsample taken 29 October 2009 All taxonomic determinations by MRD were confirmed by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson K McCann A Fisher H Kepple A Barrett
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 2 17 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Sphaeriidae 2 17 I MRD BVW CF 8 BU Isopoda (isopods) Asellidae 99 853 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Calopterygidae 2 17 A MRD BVW PR 7 CL Ephemeroptera (mayflies) Leptophlebiidae 2 17 I MRD BVW CG 2 CR Heptageniidae 4 34 A MRD BVW SC 3 CG Plecoptera (stoneflies) Perlidae 1 09 A MRD BVW PR 1 CR Trichoptera (caddisflies) Hemiptera (true bugs) (Gerridae) (1) - A MRD BVW ( ) Corixidae 1 09 A MRD BVW PR 5 CR Megaloptera (dobsonflies etc) Coleoptera (beetles) Dryopidae 1 09 A MRD BVW SC 5 CG Diptera (true flies) Tipulidae 2 17 I BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
mm
nn
Other Total Individuals 116
(117) 999
Total Taxa 10 (11)
Notes Subsample taken 6 November 2008 Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009 Gerridae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
- Disclaimer ii
- Title Page iii
- Acknowledgements v
- Table of Contents vii
- SECTION 2 RESULTS DATA TABLES 7
- OVERVIEW AND FINDINGS
- Introduction
- RESULTS DATA TABLES
- REFERENCES
- FIELD DATA SHEETS
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- SubstrateType
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- WATER QUALITY ANALYSIS
- THROUGH THE VIRGINIA
- STREAM CONDITION INDEX (VSCI)
- LABORATORY BENCH SHEETS
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
16
a
Appendix A
FIELD DATA SHEETS
This appendix presents a Site Characterization Field Data Sheet for each of the six sampling reaches established on Hiatt Run and Lick Run for this study They were filled out during the sampling runs made in May 2008
These forms were adapted by Shenandoah University from forms used by the EPA and Virginia DEQ for recording characteristics of sampling sites
b
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Mouth STATION _HLR-1_____ STREAM CLASS LAT N 39 12749 (upstream) LONG W 078 04209(upstream) LAT N 39 12729 (downstream) LONG W 078 04148 (downstream) FORM COMPILED BY Jeremy Tovar RIVER BASIN Potomac River INVESTIGATORS JPangle B Millholland MDahl SRobertson W Bousquet JTovar BBousquet
DATE May 21 2008_________ TIME_250 PM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature __24___degC showers (intermittent) Comments _______________________
___ cloud cover _0_ ______________________________
X clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x ( Significant springs andor seeps) x (Valley ) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS
c
d
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence x FieldPasture Industrial Obvious sources Agricultural Other ____________ Stream scour steep slope erosion x Residential Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left Field and young thin deciduous forest 100 Bank=Grass cobble and shrub Right 90 maturing deciduous forest 45 shale slope Bank=Bedrock soil and herbs Indicate the dominant vegetation type(s) and record the dominant species
x Trees Shrubs x (Grasses) Forbs Dominant species Oak species and mix deciduous
INSTREAM FEATURES
Measured reach length ___100__m Canopy Cover Estimated stream width _35__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area __350_m2
Estimated stream depth _008_m Proportion of Reach Represented by Stream High water mark _15_m Morphology Types Surface velocity (at thalweg) _05__msec x Riffle__75___ x Run__20___ x Pool__5___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___00__ m2 Density of LWD _-_ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __15_____ x Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present Algae
WATER QUALITY Temperature __19___degC Water Odors x NormalNone Sewage Specific Conductance - Petroleum Chemical Fishy Other___________________ Dissolved Oxygen - Water Surface Oils Slick Sheen Globs Flecks pH __-_ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used _____NA________ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic x None Relict shells Other______None_____ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 8 Detritus sticks wood coarse plant materials
0 Boulder gt256 mm (10rsquorsquo) 0
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 51 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 40 Sand 006-2 mm (gritty) 1 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University
SITE CHARACTERIZATION FIELD DATA SHEET FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Package Plant STATION HLR-2 STREAM CLASS LAT N 39 129580 (upstream) LONG W 078 04900 (upstream) LAT N 39 129581 (downstream) LONG W 078 04822 (downstream) FORM COMPILED BY JW Pangle RIVER BASIN Potomac River INVESTIGATORS JPangle JTovar MDahl B Millholland SRobertson W Bousquet
DATE__19 May 2008_ TIME__1240__ PM
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature _____degC showers (intermittent) Comments __Sunny Day_____________
_5__ cloud cover _0__ ______________________________
x clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x (Significant springs andor seeps) x (Valley) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS
e
f
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence FieldPasture Industrial Obvious sources Agricultural Other ____________ __Upstream Construction and Stream bank Scour Residential __________________________________ Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left Deciduous forest with thick herb and shrub layers bank= vegetation and rocks Right Deciduous forest with thick herb and shrub layers bank=vegetation soil and rocks Indicate the dominant vegetation type(s) and record the dominant species
x Trees x Shrubs Grasses Forbs Dominant species Black Walnut White Ash Hackberry and American Sycamore
INSTREAM FEATURES
Measured reach length 100 m Canopy Cover Estimated stream width _35__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area __350_m2
Estimated stream depth __15__m Proportion of Reach Represented by Stream High water mark __22___m Morphology Types Surface velocity (at thalweg) _035__msec x Riffle__30___ x Run__30___ x Pool__40___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___10____ m2 Density of LWD _______ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __30___ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present__________________________________________________________
WATER QUALITY Temperature __18___degC Water Odors x NormalNone Sewage Specific Conductance __-____ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ____-____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used _____NA____ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other___None_____ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach SubstrateType Characteristic
Composition in Sampling Reach
Bedrock 3 Detritus sticks wood coarse plant materials
2 Boulder gt256 mm (10rsquorsquo) 5
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 70 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 22 Sand 006-2 mm (gritty) 0 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiattrsquos Run LOCATION Rt 664 Bridge STATION HLR-3 STREAM CLASS LAT N 39 12934 (upstream) LONG W 078 05019 (upstream) LAT N 39 12958 (downstream) LONG W 078 04958 (downstream) FORM COMPILED BY Sean Robertson RIVER BASIN Potomac River INVESTIGATORS SRobertson BMillholland WBousquet MDahl JPangle
DATE 28 May 2008 TIME 1127 AM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality 2008
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature __14___degC showers (intermittent) x Trace Comments _______________________
_65 x cloud cover ___ ______________________________
x clearsunny __________________________________
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x (Significant springs andor seeps) x (Valley) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS
g
h
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence Some evidence x FieldPasture Industrial x Obvious sources Agricultural x Other Old Package Plant Downstream x Residential Bridge and Road Power line and Pump station Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left 70 field 30 shrubs and deciduous trees Bank=Soil and vegetation Right Maturing deciduous forest steep slope Bank=Shale Bedrock Indicate the dominant vegetation type(s) and record the dominant species
x Trees x Shrubs x Grasses Forbs Dominant species Sycamore and Red Oaks
INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width __51___m Fully open x Partly open Partly shaded Fully shaded Sampling reach area _510_m2
Estimated stream depth __135___m Proportion of Reach Represented by Stream High water mark __-___m Morphology Types Surface velocity (at thalweg) __20___msec x Riffle_40_ x Run_50_ x Pool 10 Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___0____ m2 Density of LWD ___-____ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation ___50____ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present Brown Algae
WATER QUALITY Temperature __17___degC Water Odors x NormalNone Sewage Specific Conductance ___-___ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ___-_____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used ____NA_______ Clear x Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other____None____ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 15 Detritus sticks wood coarse plant materials
0 Boulder gt256 mm (10rsquorsquo) 15
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 62 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 8 Sand 006-2 mm (gritty) 0 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Unnamed Tributary STATION HLR-4 STREAM CLASS LAT N 39 13035 (upstream) LONG W 078 05129 (upstream) LAT N 39 13000 (downstream) LONG W 078 05086 (downstream) FORM COMPILED BY Marie Dahl RIVER BASIN Potomac River INVESTIGATORS M Dahl HKepple SRobertson AFisher
DATE 31 May 2008 TIME 5 PM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality 2008
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) YES x NO
rain (steady rain) Air Temperature __22___degC showers (intermittent) Comments _______________________
_0__ cloud cover _0__ ______________________________
x clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area __-___ km2 mi2 Montane Significant springs andor seeps x Valley Marsh or swamp Montane amp Valley Mixture of origins Other ___________________
COMMENTS Snowden Bridge development (upstream) and sewer line
i
j
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence x FieldPasture Industrial Obvious sources Agricultural Other ____________ Construction upstream and adjacent x Residential Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left Forested with rocky banks field across silt fence and 2m tree strip Right Forested with some rocky banks and forest through the highway Indicate the dominant vegetation type(s) and record the dominant species
x Trees x Shrubs x Grasses Forbs Dominant species White Ash and Hackberry
INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width ___38__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area __380__m2
Estimated stream depth __12__cm Proportion of Reach Represented by Stream High water mark __2___m Morphology Types Surface velocity (at thalweg) __46___msec x Riffle__25___ x Run__70___ x Pool__5___ Channelized Yes x No Dam Present x Yes No
LARGE WOODY DEBRIS
LWD _______ m2 Density of LWD _______ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation ___NA____ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present___Algae___
WATER QUALITY Temperature __16___degC Water Odors x NormalNone Sewage Specific Conductance __-____ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ___-_____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used ____NA_____ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells x Other___Shale_______ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 0 Detritus sticks wood coarse plant materials
10 Boulder gt256 mm (10rsquorsquo) 5
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 45 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 30 Sand 006-2 mm (gritty) 0 Marl grey shell
fragments 0
Silt 0004-006 mm 20 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Lick Run STATION HLR-5 STREAM CLASS LAT N 39 127517 (upstream) LONG W 078 05685(upstream) LAT N 39 12784 (downstream) LONG W 078 05631 (downstream) FORM COMPILED BY B Millholland RIVER BASIN Potomac River INVESTIGATORS B Millholland JTovar SRobertson MDahl WBousquet JPangle
DATE 29 May 2008 TIME 1040 AM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project
k
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature __15___degC showers (intermittent) Comments _______________________
_5_ X cloud cover _0__ ______________________________
x clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane Significant springs andor seeps x Valley Marsh or swamp Montane amp Valley Mixture of origins Other ___________________
COMMENTS Lick Run appears to be intermittent and may be dry in Fall 2008
l
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence x FieldPasture Industrial Obvious sources Agricultural Other ____________ Adjacent logging stream bank scour Residential Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left 85 young deciduous forest 15 logged Bank=Soil vegetation and cobbles Right Young deciduous forest on 30 slope Bank= Soil vegetation and cobble Indicate the dominant vegetation type(s) and record the dominant species
x Trees Shrubs Grasses Forbs Dominant species Mixed Deciduous INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width __11__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area 110 m2
Estimated stream depth _009__m Proportion of Reach Represented by Stream High water mark __96___m Morphology Types Surface velocity (at thalweg) __01___msec x Riffle__30___ x Run__5___ x Pool__65___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___0____ m2 Density of LWD ___-____ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __70___ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present__________________________________________________________
WATER QUALITY Temperature __14___degC Water Odors x NormalNone Sewage Specific Conductance __-__ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ___-___ Water Surface Oils Slick Sheen Globs Flecks pH __-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used _____NA______ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other_____None_____
SEDIMENT AND SUBSTRATE
Odors Deposits Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other________________ x Other Slightly Salty Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 0 Detritus sticks wood coarse plant materials
5 Boulder gt256 mm (10rsquorsquo) 0
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 75 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 23 Sand 006-2 mm (gritty) 2 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION McCann Farm STATION HLR-6 STREAM CLASS LAT N 39 13076 (upstream) LONG W 078 06539 (upstream) LAT N 39 13063 (downstream) LONG W 078 06473 (downstream) FORM COMPILED BY MDahl RIVER BASIN Potomac River INVESTIGATORS MDahl JPangle SRobertson W Bousqet BMillholland JTovar H Kepple
DATE 30 May 2008 TIME 1050 AM (start)
PROJECT NAME
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) YES x NO
rain (steady rain) Air Temperature __18___degC showers (intermittent) Comments _______________________
_85_x cloud cover _10__ ______________________________
clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x (Significant springs andor seeps) x ( Valley) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS Multi-habitat sampling with D-frame nets and seine
m
n
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial x No evidence Some evidence x FieldPasture Industrial Obvious sources x Agricultural Other ____________ Minor stream bank scour Residential Local Watershed Erosion x None Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left 35 bank grasses scattered trees and some bushes Right High bank with grasses and scattered trees Indicate the dominant vegetation type(s) and record the dominant species
x Trees Shrubs Grasses Forbs Dominant species Black Walnut and Festuca elatior
INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width __2___m Fully open Partly open x Partly shaded Fully shaded Sampling reach area __200_m2
Estimated stream depth __03_m Proportion of Reach Represented by Stream High water mark __1___m Morphology Types Surface velocity (at thalweg) __166_msec x Riffle__0___ x Run__55___ x Pool__45___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___2____ m2 Density of LWD ___-____ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __20_____ x Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present ____Glyceria (grass)sp__________
WATER QUALITY Temperature __16___degC Water Odors x NormalNone Sewage Specific Conductance ___-___ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ____-____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used ______NA___ Clear x Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other__NONE__ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 0 Detritus sticks wood coarse plant materials
5 Boulder gt256 mm (10rsquorsquo) 0
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 27 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 38 Sand 006-2 mm (gritty) 5 Marl grey shell
fragments 0 (lt1)
Silt 0004-006 mm 25 Clay lt 0004 mm (slick) 5 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Appendix B
WATER QUALITY ANALYSIS THROUGH THE VIRGINIA
STREAM CONDITION INDEX (VSCI)
The Virginia Stream Condition Index (VSCI) relies on eight metrics to categorize stream health According to Burton and Gerritsen (2003 p 1-1) this index ldquohellip can be used as a primary indicator of ecosystem health and can identify impairment with respect to the reference (or natural) conditionrdquo These metrics are numeric measures of a biological communityrsquos species composition diversity ecological niches or tolerance to pollution
Table 13 on the next page describes each VSCI metric lists its formula and provides the best standard value (BSV) for that metric Based on a 100-point scale the BSV is the score that reflects optimum conditions for aquatic life ie the best water quality value possible (DEQ 2006b pp 8 33) The table also explains whether the score increases or decreases with environmental stress
As Table 13 indicates each of the eight VSCI metrics is calculated individually Each formula provides a unitless score based on a 100-point scale with 0 as the worst possible score and 100 as the best Any metric that provides a result above 100 is changed to 100 in order for the index to work properly After the eight metrics are calculated they are summed and averaged to provide a final VSCI score on a 100-point scale
Based on statistical analysis of Virginiarsquos streams and their BMI communities the DEQ (2006b p 8) currently uses the following tiers to interpret VSCI scores
VSCI Scores Aquatic Life Use
(ALI) Tiers
lt 42 severely stressed
42-55 moderately stressed
55-63 unknownfair (gray zone)
63-73 good
gt73 excellent
Section 2 of this report presents the results of calculations for the six BMI sampling sites the research team studied in the Hiatt-Lick Run subwatershed in 2008
o
p
1 Total taxa the number of distinct taxa in the sample measures the overall diversity of the macroinvertebrate assemblage The best standard value (BSV) is 189 Score = 100 x (X189) Total taxa decreases with stress 2 EPT taxa the sum of the distinct taxa in the orders Ephemeroptera (mayflies) Plecoptera (stoneflies) and Trichoptera (caddisflies) These orders are known to be sensitive to pollution The best standard value (BSV) is 117 Score = 100 x (X117) EPT taxa decreases with stress 3 Percent Ephemeroptera the percent abundance of individuals in the sample that belong to the order Ephemeroptera (mayflies) The best standard value (BSV) is 477 Score = 100 x (X477) Percent Ephemeroptera decreases with stress 4 Percent Plecoptera + Trichoptera less Hydropsychidae the percent abundance of individuals in the sample that belong to the orders Plecoptera (stoneflies) and Trichoptera (caddisflies) excluding the pollution-tolerant caddisfly family Hydropsychidae The best standard value (BSV) is 562 Score = 100 x (X562) Percent Plecoptera + Trichoptera less Hydropsychidae decreases with stress 5 Percent Scrapers the percent abundance of individuals in the sample whose primary functional mechanism for feeding is grazing on the substrate or periphyton (ie attached algae and associated material) The best standard value (BSV) is 467 Score = 100 x (X467) Percent scrapers decreases with stress 6 Percent Chironomidae the percent abundance of individuals in the sample that are Chironomidae (non-biting midge) larvae and pupae The best standard value (BSV) is 16 Score = 100 x [(100-X)(100-16)] Percent Chironomidae increases with stress 7 Percent Top Two Dominant the percent abundance of the two most frequent taxa present in the sample The best standard value (BSV) is 328 Score = 100 x [(100-X)(100-328)] The Percent Top 2 Dominant increases with stress 8 Hilsenhoff Biotic Index (HBI) an abundance-weighted average pollution tolerance of all the individuals within a sample keyed to the family level Tolerance ratings range from 1 (highly intolerant) to 10 (highly tolerant) The best standard value (BSV) is 29 Score = 100 x [(10-X)(10-29)] The Hilsenhoff Biotic Index (HBI) increases with stress ______________________________________________________________________________ Table 13 Description of Benthic Macroinvertebrate VSCI Metrics Includes responses (increases decreases) of each VSCI metric to degraded water quality conditions Adapted from Burton and Gerritsen (2003 pp 3-15 3-16 6-2) and DEQ (2008)
q
Appendix C
LABORATORY BENCH SHEETS
Shenandoah Universityrsquos Environmental Studies Department developed the Benthic Macroinvertebrate Laboratory Bench Sheet and VSCI Worksheet to record BMI specimen identifications and their VSCI classifications for each sample
This form is based in part on bench sheets developed by the EPA and Virginia DEQ for recording specimen identifications Additional columns on Shenandoah Universityrsquos forms for relative numbers and VSCI classifications make the calculation and verification of VSCI scores easier
Note the column for taxonomistrsquos initials Specimens were identified by students in Shenandoah Universityrsquos research group and then verified or where necessary changed by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality This external review helps assure the validity of the study Originals of these bench sheets with handwritten taxonomistsrsquo initials will be kept on file at Shenandoah Universityrsquos Environmental Studies Department for five years after the completion of this study
r
s
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-1 STATION NAME Mouth LAT N 39deg 12749rsquo (upstream) LONG W 078deg 04209rsquo (upstream) LAT N 39deg 12729rsquo (downstream) LONG W 078deg 04148rsquo (downstream) FORM COMPILED BY Jeremy Tovar W S Bousquet RIVER BASIN Potomac River INVESTIGATORS Marie Dahl Brandon Millholand J W Pangle Sean Robertson Jeremy Tovar Woodward Bousquet Ben Bousquet
Collection date 21 May 2008 Sheet completion date 12 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Hirudinea 3 33 JT BVW PR 8 SP Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Coenagrionidae 2 22 BVW PR 9 CR Ephemeroptera (mayflies) Caenidae 1 11 JT BVW CG 6 SP Ephemerllidae 4 44 JT BVW CG 2 CR Heptageniidae 5 55 JT BVW SC 3 CG Isonychiidae 1 11 BVW CF 3 CG Plecoptera (stoneflies) Perlidae 14 154 JT BVW PR 2 CR Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Corydalidae 1 11 JT BVW PR 5 CR Coleoptera (beetles) Elmidae 24 264 I JT BVW SC 4 CG Dytiscidae 4 44 JT BVW PR 6 GN Psephenidae 3 33 JT BVW SC 4 CG Diptera (true flies) Tipulidae 3 33 JT BVW SH 4 BU Chironomidae 26 286 JT BVW CG 6 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
t
Other Total Individuals 91 1001 Total Taxa 13
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
u
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-1 STATION NAME Mouth LAT N 39deg 12749rsquo (upstream) LONG W 078deg 04209rsquo (upstream) LAT N 39deg 12729rsquo (downstream) LONG W 078deg 04148rsquo (downstream) FORM COMPILED BY Jeremy Tovar W S Bousquet RIVER BASIN Potomac River INVESTIGATORS Brandon Millholand JW Pangle Jeremy Tovar Heather Kepple
Collection date 10 October 2008 Sheet completion date 12 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Oligochaeta 3 35 JT BVW CG 8 BU Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Coenagrionidae 2 23 JT BVW PR 9 CR Ephemeroptera (mayflies) Heptageniidae 12 140 JT BVW SC 3 CG Isonychiidae 2 23 BVW CF 3 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Hydropsychidae 16 186 JT BVW CF 6 CG Philopotamidae 31 360 JT BVW CF 2 CG Hemiptera (true bugs) (Veliidae) (5) - JT BVW ( ) Megaloptera (dobsonflies etc) Corydalidae 10 116 JT BVW PR 5 CR Coleoptera (beetles) Dytiscidae 1 12 JT BVW PR 6 GN Psephenidae 7 81 JT BVW SC 4 CG Diptera (true flies) Tipulidae 2 23 BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
v
Other Total Individuals 86
(91) 999
Total Taxa 10 (11)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009 Veliidae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
w
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-2 STATION NAME Package Plant LAT N 39deg 12958rsquo (upstream) LONG W 078deg 04900 (upstream) LAT N 39deg 12958rsquo (downstream) LONG W 078deg 04822 (downstream) FORM COMPILED BY JW Pangle W Bousquet RIVER BASIN Potomac River INVESTIGATORS JW Pangle S Robertson J Tovar M Dahl B Millholland W Bousquet
Collection date 29 May 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Plecoptera (stoneflies) Perlidae 11 136 I JP BVW PR 1 CR Trichoptera (caddisflies) Hydropsychidae 10 123 I JP BVW CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 14 173 I JP BVW SC 4 CG Diptera (true flies) Chironomidae 32 395 I JP BVW CG 6 BU Simuliidae 14 173 I JP BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
x
Other Total Individuals 81 1000 Total Taxa 5
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-2 STATION NAME Package Plant LAT N 39deg 12958rsquo (upstream) LONG W 078deg 04900 (upstream) LAT N 39deg 12958rsquo (downstream) LONG W 078deg 04822 (downstream) FORM COMPILED BY JW Pangle W Bousquet RIVER BASIN Potomac River INVESTIGATORS JW Pangle Brandon Millholland Jeremy Tovar Heather Kepple
Collection date 10 October 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Heptageniidae 40 392 I WBJP SC 3 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Hydropsychidae 7 69 I JP WB BVW CF 6 CG Philopotamidae 28 275 I JP WB BVW CF 2 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Corydalidae 6 59 I JP WB BVW PR 5 CR Coleoptera (beetles) Psephenidae 7 69 I JP WB BVW SC 4 CG Elmidae 8 78 I JP WB BVW SC 4 CG Diptera (true flies) Tipulidae 6 59 I JP WB BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
y
z
Other Total Individuals 102 1001 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-3 STATION NAME Rt 664 Bridge LAT N 39deg 12934rsquo (upstream) LONG W 078deg 05019rsquo (upstream) LAT N 39deg 12951rsquo (downstream) LONG W 078deg 04958rsquo (downstream) FORM COMPILED BY S Robertson W Bousquet RIVER BASIN Potomac River INVESTIGATORS SRobertson BMillholland WBousquet MDahl JPangle
Collection date 28 May 2008 Sheet completion date 11 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Leptophlebiidae 1 15 I SGR BVW CG 2 CR Baetidae 1 15 I BVW CG 5 CG Plecoptera (stoneflies) Perlidae 3 46 I SGR BVW PR 1 CR Nemouridae 1 15 I BVW SH 3 CR Trichoptera (caddisflies) Hydropsychidae 5 77 I SGR BVW CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 27 415 I SGR BVW SC 4 CG Psephenidae 2 31 A SGR BVW SC 4 CG Diptera (true flies) Simuliidae 5 77 I SGR BVW CF 6 CG Chironomidae 20 308 I SGR BVW CG 6 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
aa
bb
Other Total Individuals 65 999 Total Taxa 9
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-3 STATION NAME Rt 664 Bridge LAT N 39deg 12934rsquo (upstream) LONG W 078deg 05019rsquo (upstream) LAT N 39deg 12951rsquo (downstream) LONG W 078deg 04958rsquo (downstream) FORM COMPILED BY S Robertson RIVER BASIN Potomac River INVESTIGATORS S Robertson M Dahl A Fisher H Kepple
Collection date 10 Oct 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Heptageniidae 2 20 I SGR SC 3 CG Isonychiidae 2 20 I BVW CF 2 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Philopotamidae 36 367 I SGR CF 2 CG Hydropsychidae 47 480 I SGR CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 2 20 A SGR SC 4 CG Psephenidae 8 82 A SGR SC 4 CG Diptera (true flies) Tabanidae 1 10 I SGR PR 7 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
cc
dd
Other Total Individuals 98 999 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-4 STATION NAME Unnamed Tributary LAT (upstream) N 39deg 13035rsquo LONG (upstream) W 078deg 05129rsquo LAT (downstream) N 39deg 13000rsquo LONG (downstream) W 078deg 5086rsquo FORM COMPILED BY A Fisher M Dahl rev W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson A Fisher H Kepple
Collection date 31 May 2008 Sheet completion date rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Flatworms (Turbellaria) Worms (Oligochaeta) Naididae 1 14 - BVW CG 8 BU Leeches (Hirudinea) Snails (Gastropoda) Clams (Bivalvia) Isopods (Isopoda) Asellidae 3 42 A AF BVW CG 8 SP Scuds (Amphipoda) Crayfish Shrimp (Decapoda) Water Mites (Hydracarina) Dragonflies (Odonata Anisoptera) Damselflies (Odonata Zygoptera) Mayflies (Ephemeroptera) Stoneflies (Plecoptera) Perlidae 2 28 I AF BVW PR 1 CR Caddisflies (Trichoptera) True Bugs (Hemiptera) Corixidae 1 14 I AF BVW MP 9 DV Dobsonflies (Megaloptera) Beetles (Coleoptera) Dytiscidae 1 14 I AF BVW PR 6 GN True Flies (Diptera) Chironomidae 61 847 I AF BVW CG 6 BU Simuliidae 3 42 I AF BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
ee
ff
Other Total Individuals 72 1001 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-4 STATION NAME Unnamed Tributary LAT (upstream) N 39deg 13035rsquo LONG (upstream) W 078deg 05129rsquo LAT (downstream) N 39deg 13000rsquo LONG (downstream) W 078deg 5086rsquo FORM COMPILED BY Andrew G M Fisher W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson A Fisher H Kepple
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Flatworms (Turbellaria) Worms (Oligochaeta) Lumbriculidae 2 32 A AF BVW CG 8 BU Leeches (Hirudinea) Snails (Gastropoda) Lymnaeidae 1 16 A BVW CG 9 GN Clams (Bivalvia) Isopods (Isopoda) Scuds (Amphipoda) Crayfish Shrimp (Decapoda) Cambaridae 1 16 A AF BVW GN 5 GN Water Mites (Hydracarina) Dragonflies (Odonata Anisoptera) Damselflies (Odonata Zygoptera) Calopterygidae 4 63 I BVW PR 7 CL Mayflies (Ephemeroptera) Tricorythidae 1 16 I BVW CG 5 SP Stoneflies (Plecoptera) Caddisflies (Trichoptera) Phryganeidae 4 63 I AF BVW SH 4 CR Hydropsychidae 6 95 I AF BVW CF 6 CG I True Bugs (Hemiptera) Corixidae 8 127 I AF BVW MP 9 DV (Veliidae ) (7) - I AF BVW ( ) Dobsonflies (Megaloptera) Sialidae 3 48 I AF BVW PR 7 SP Corydalidae 2 32 I AF BVW PR 5 CR Beetles (Coleoptera) Haliplidae 1 16 A AF BVW MP 8 GN Dysticidae 7 111 I AF BVW PR 6 GN Hydrophilidae 1 16 A AF BVW PR 8 GN Elmidae 3 48 I BVW SC 4 CG True Flies (Diptera) Chironomidae 8 127 I AF BVW CG 6 BU Simuliidae 7 111 I AF BVW CF 6 CG Tipulidae 4 63 I AF BVW SH 4 GU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
gg
hh
Other Total Individuals 63
(70) 1000
Total Taxa 17 (18)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Veliidae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-5 STATION NAME Lick Run LAT N 39deg 12752rsquo (upstream) LONG W 078deg 05685rsquo (upstream) LAT N 39deg 12784rsquo (downstream) LONG W 078deg 05631rsquo (downstream) FORM COMPILED BY Brandon Millholland W Bousquet RIVER BASIN Potomac River INVESTIGATORS J Pangle S Robertson W Bousquet JTovar M Dahl
Collection date 29 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Gomphidae 1 15 I BVW PR 3 BU Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Leptophlebiidae 11 167 A LBM BVW CG 2 CR Plecoptera (stoneflies) Perlidae 10 152 A LBM BVW PR 1 CR Perlodiae 1 15 A LBM BVW PR 2 CR Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 5 76 A LBM BVW SC 4 CG Elmidae 2 30 I LBM BVW SC 4 CG Diptera (true flies) (Culicidae) (3) - P LMB BVW ( ) Chironomidae 23 348 I LBM BVW CG 6 BU Simuliidae 13 197 I LBM BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
ii
jj
Other Total Individuals 66
(69) 1000
Total Taxa 7 (8)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Culicidae (Diptera) pupae not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl J Pangle S Robertson W Bousquet B Millholland J Tovar H Kepple
Collection date 30 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 1 12 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Asellidae 114 965 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Plecoptera (stoneflies) Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 2 23 A MRD BVW SC 4 CG Diptera (true flies)
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
kk
ll
Other Total Individuals 117 1000 Total Taxa 3
Notes Subsample taken 29 October 2009 All taxonomic determinations by MRD were confirmed by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson K McCann A Fisher H Kepple A Barrett
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 2 17 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Sphaeriidae 2 17 I MRD BVW CF 8 BU Isopoda (isopods) Asellidae 99 853 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Calopterygidae 2 17 A MRD BVW PR 7 CL Ephemeroptera (mayflies) Leptophlebiidae 2 17 I MRD BVW CG 2 CR Heptageniidae 4 34 A MRD BVW SC 3 CG Plecoptera (stoneflies) Perlidae 1 09 A MRD BVW PR 1 CR Trichoptera (caddisflies) Hemiptera (true bugs) (Gerridae) (1) - A MRD BVW ( ) Corixidae 1 09 A MRD BVW PR 5 CR Megaloptera (dobsonflies etc) Coleoptera (beetles) Dryopidae 1 09 A MRD BVW SC 5 CG Diptera (true flies) Tipulidae 2 17 I BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
mm
nn
Other Total Individuals 116
(117) 999
Total Taxa 10 (11)
Notes Subsample taken 6 November 2008 Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009 Gerridae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
- Disclaimer ii
- Title Page iii
- Acknowledgements v
- Table of Contents vii
- SECTION 2 RESULTS DATA TABLES 7
- OVERVIEW AND FINDINGS
- Introduction
- RESULTS DATA TABLES
- REFERENCES
- FIELD DATA SHEETS
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- SubstrateType
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- WATER QUALITY ANALYSIS
- THROUGH THE VIRGINIA
- STREAM CONDITION INDEX (VSCI)
- LABORATORY BENCH SHEETS
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
a
Appendix A
FIELD DATA SHEETS
This appendix presents a Site Characterization Field Data Sheet for each of the six sampling reaches established on Hiatt Run and Lick Run for this study They were filled out during the sampling runs made in May 2008
These forms were adapted by Shenandoah University from forms used by the EPA and Virginia DEQ for recording characteristics of sampling sites
b
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Mouth STATION _HLR-1_____ STREAM CLASS LAT N 39 12749 (upstream) LONG W 078 04209(upstream) LAT N 39 12729 (downstream) LONG W 078 04148 (downstream) FORM COMPILED BY Jeremy Tovar RIVER BASIN Potomac River INVESTIGATORS JPangle B Millholland MDahl SRobertson W Bousquet JTovar BBousquet
DATE May 21 2008_________ TIME_250 PM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature __24___degC showers (intermittent) Comments _______________________
___ cloud cover _0_ ______________________________
X clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x ( Significant springs andor seeps) x (Valley ) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS
c
d
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence x FieldPasture Industrial Obvious sources Agricultural Other ____________ Stream scour steep slope erosion x Residential Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left Field and young thin deciduous forest 100 Bank=Grass cobble and shrub Right 90 maturing deciduous forest 45 shale slope Bank=Bedrock soil and herbs Indicate the dominant vegetation type(s) and record the dominant species
x Trees Shrubs x (Grasses) Forbs Dominant species Oak species and mix deciduous
INSTREAM FEATURES
Measured reach length ___100__m Canopy Cover Estimated stream width _35__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area __350_m2
Estimated stream depth _008_m Proportion of Reach Represented by Stream High water mark _15_m Morphology Types Surface velocity (at thalweg) _05__msec x Riffle__75___ x Run__20___ x Pool__5___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___00__ m2 Density of LWD _-_ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __15_____ x Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present Algae
WATER QUALITY Temperature __19___degC Water Odors x NormalNone Sewage Specific Conductance - Petroleum Chemical Fishy Other___________________ Dissolved Oxygen - Water Surface Oils Slick Sheen Globs Flecks pH __-_ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used _____NA________ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic x None Relict shells Other______None_____ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 8 Detritus sticks wood coarse plant materials
0 Boulder gt256 mm (10rsquorsquo) 0
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 51 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 40 Sand 006-2 mm (gritty) 1 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University
SITE CHARACTERIZATION FIELD DATA SHEET FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Package Plant STATION HLR-2 STREAM CLASS LAT N 39 129580 (upstream) LONG W 078 04900 (upstream) LAT N 39 129581 (downstream) LONG W 078 04822 (downstream) FORM COMPILED BY JW Pangle RIVER BASIN Potomac River INVESTIGATORS JPangle JTovar MDahl B Millholland SRobertson W Bousquet
DATE__19 May 2008_ TIME__1240__ PM
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature _____degC showers (intermittent) Comments __Sunny Day_____________
_5__ cloud cover _0__ ______________________________
x clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x (Significant springs andor seeps) x (Valley) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS
e
f
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence FieldPasture Industrial Obvious sources Agricultural Other ____________ __Upstream Construction and Stream bank Scour Residential __________________________________ Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left Deciduous forest with thick herb and shrub layers bank= vegetation and rocks Right Deciduous forest with thick herb and shrub layers bank=vegetation soil and rocks Indicate the dominant vegetation type(s) and record the dominant species
x Trees x Shrubs Grasses Forbs Dominant species Black Walnut White Ash Hackberry and American Sycamore
INSTREAM FEATURES
Measured reach length 100 m Canopy Cover Estimated stream width _35__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area __350_m2
Estimated stream depth __15__m Proportion of Reach Represented by Stream High water mark __22___m Morphology Types Surface velocity (at thalweg) _035__msec x Riffle__30___ x Run__30___ x Pool__40___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___10____ m2 Density of LWD _______ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __30___ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present__________________________________________________________
WATER QUALITY Temperature __18___degC Water Odors x NormalNone Sewage Specific Conductance __-____ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ____-____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used _____NA____ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other___None_____ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach SubstrateType Characteristic
Composition in Sampling Reach
Bedrock 3 Detritus sticks wood coarse plant materials
2 Boulder gt256 mm (10rsquorsquo) 5
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 70 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 22 Sand 006-2 mm (gritty) 0 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiattrsquos Run LOCATION Rt 664 Bridge STATION HLR-3 STREAM CLASS LAT N 39 12934 (upstream) LONG W 078 05019 (upstream) LAT N 39 12958 (downstream) LONG W 078 04958 (downstream) FORM COMPILED BY Sean Robertson RIVER BASIN Potomac River INVESTIGATORS SRobertson BMillholland WBousquet MDahl JPangle
DATE 28 May 2008 TIME 1127 AM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality 2008
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature __14___degC showers (intermittent) x Trace Comments _______________________
_65 x cloud cover ___ ______________________________
x clearsunny __________________________________
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x (Significant springs andor seeps) x (Valley) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS
g
h
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence Some evidence x FieldPasture Industrial x Obvious sources Agricultural x Other Old Package Plant Downstream x Residential Bridge and Road Power line and Pump station Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left 70 field 30 shrubs and deciduous trees Bank=Soil and vegetation Right Maturing deciduous forest steep slope Bank=Shale Bedrock Indicate the dominant vegetation type(s) and record the dominant species
x Trees x Shrubs x Grasses Forbs Dominant species Sycamore and Red Oaks
INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width __51___m Fully open x Partly open Partly shaded Fully shaded Sampling reach area _510_m2
Estimated stream depth __135___m Proportion of Reach Represented by Stream High water mark __-___m Morphology Types Surface velocity (at thalweg) __20___msec x Riffle_40_ x Run_50_ x Pool 10 Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___0____ m2 Density of LWD ___-____ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation ___50____ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present Brown Algae
WATER QUALITY Temperature __17___degC Water Odors x NormalNone Sewage Specific Conductance ___-___ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ___-_____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used ____NA_______ Clear x Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other____None____ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 15 Detritus sticks wood coarse plant materials
0 Boulder gt256 mm (10rsquorsquo) 15
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 62 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 8 Sand 006-2 mm (gritty) 0 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Unnamed Tributary STATION HLR-4 STREAM CLASS LAT N 39 13035 (upstream) LONG W 078 05129 (upstream) LAT N 39 13000 (downstream) LONG W 078 05086 (downstream) FORM COMPILED BY Marie Dahl RIVER BASIN Potomac River INVESTIGATORS M Dahl HKepple SRobertson AFisher
DATE 31 May 2008 TIME 5 PM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality 2008
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) YES x NO
rain (steady rain) Air Temperature __22___degC showers (intermittent) Comments _______________________
_0__ cloud cover _0__ ______________________________
x clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area __-___ km2 mi2 Montane Significant springs andor seeps x Valley Marsh or swamp Montane amp Valley Mixture of origins Other ___________________
COMMENTS Snowden Bridge development (upstream) and sewer line
i
j
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence x FieldPasture Industrial Obvious sources Agricultural Other ____________ Construction upstream and adjacent x Residential Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left Forested with rocky banks field across silt fence and 2m tree strip Right Forested with some rocky banks and forest through the highway Indicate the dominant vegetation type(s) and record the dominant species
x Trees x Shrubs x Grasses Forbs Dominant species White Ash and Hackberry
INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width ___38__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area __380__m2
Estimated stream depth __12__cm Proportion of Reach Represented by Stream High water mark __2___m Morphology Types Surface velocity (at thalweg) __46___msec x Riffle__25___ x Run__70___ x Pool__5___ Channelized Yes x No Dam Present x Yes No
LARGE WOODY DEBRIS
LWD _______ m2 Density of LWD _______ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation ___NA____ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present___Algae___
WATER QUALITY Temperature __16___degC Water Odors x NormalNone Sewage Specific Conductance __-____ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ___-_____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used ____NA_____ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells x Other___Shale_______ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 0 Detritus sticks wood coarse plant materials
10 Boulder gt256 mm (10rsquorsquo) 5
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 45 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 30 Sand 006-2 mm (gritty) 0 Marl grey shell
fragments 0
Silt 0004-006 mm 20 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Lick Run STATION HLR-5 STREAM CLASS LAT N 39 127517 (upstream) LONG W 078 05685(upstream) LAT N 39 12784 (downstream) LONG W 078 05631 (downstream) FORM COMPILED BY B Millholland RIVER BASIN Potomac River INVESTIGATORS B Millholland JTovar SRobertson MDahl WBousquet JPangle
DATE 29 May 2008 TIME 1040 AM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project
k
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature __15___degC showers (intermittent) Comments _______________________
_5_ X cloud cover _0__ ______________________________
x clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane Significant springs andor seeps x Valley Marsh or swamp Montane amp Valley Mixture of origins Other ___________________
COMMENTS Lick Run appears to be intermittent and may be dry in Fall 2008
l
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence x FieldPasture Industrial Obvious sources Agricultural Other ____________ Adjacent logging stream bank scour Residential Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left 85 young deciduous forest 15 logged Bank=Soil vegetation and cobbles Right Young deciduous forest on 30 slope Bank= Soil vegetation and cobble Indicate the dominant vegetation type(s) and record the dominant species
x Trees Shrubs Grasses Forbs Dominant species Mixed Deciduous INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width __11__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area 110 m2
Estimated stream depth _009__m Proportion of Reach Represented by Stream High water mark __96___m Morphology Types Surface velocity (at thalweg) __01___msec x Riffle__30___ x Run__5___ x Pool__65___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___0____ m2 Density of LWD ___-____ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __70___ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present__________________________________________________________
WATER QUALITY Temperature __14___degC Water Odors x NormalNone Sewage Specific Conductance __-__ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ___-___ Water Surface Oils Slick Sheen Globs Flecks pH __-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used _____NA______ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other_____None_____
SEDIMENT AND SUBSTRATE
Odors Deposits Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other________________ x Other Slightly Salty Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 0 Detritus sticks wood coarse plant materials
5 Boulder gt256 mm (10rsquorsquo) 0
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 75 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 23 Sand 006-2 mm (gritty) 2 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION McCann Farm STATION HLR-6 STREAM CLASS LAT N 39 13076 (upstream) LONG W 078 06539 (upstream) LAT N 39 13063 (downstream) LONG W 078 06473 (downstream) FORM COMPILED BY MDahl RIVER BASIN Potomac River INVESTIGATORS MDahl JPangle SRobertson W Bousqet BMillholland JTovar H Kepple
DATE 30 May 2008 TIME 1050 AM (start)
PROJECT NAME
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) YES x NO
rain (steady rain) Air Temperature __18___degC showers (intermittent) Comments _______________________
_85_x cloud cover _10__ ______________________________
clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x (Significant springs andor seeps) x ( Valley) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS Multi-habitat sampling with D-frame nets and seine
m
n
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial x No evidence Some evidence x FieldPasture Industrial Obvious sources x Agricultural Other ____________ Minor stream bank scour Residential Local Watershed Erosion x None Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left 35 bank grasses scattered trees and some bushes Right High bank with grasses and scattered trees Indicate the dominant vegetation type(s) and record the dominant species
x Trees Shrubs Grasses Forbs Dominant species Black Walnut and Festuca elatior
INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width __2___m Fully open Partly open x Partly shaded Fully shaded Sampling reach area __200_m2
Estimated stream depth __03_m Proportion of Reach Represented by Stream High water mark __1___m Morphology Types Surface velocity (at thalweg) __166_msec x Riffle__0___ x Run__55___ x Pool__45___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___2____ m2 Density of LWD ___-____ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __20_____ x Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present ____Glyceria (grass)sp__________
WATER QUALITY Temperature __16___degC Water Odors x NormalNone Sewage Specific Conductance ___-___ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ____-____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used ______NA___ Clear x Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other__NONE__ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 0 Detritus sticks wood coarse plant materials
5 Boulder gt256 mm (10rsquorsquo) 0
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 27 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 38 Sand 006-2 mm (gritty) 5 Marl grey shell
fragments 0 (lt1)
Silt 0004-006 mm 25 Clay lt 0004 mm (slick) 5 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Appendix B
WATER QUALITY ANALYSIS THROUGH THE VIRGINIA
STREAM CONDITION INDEX (VSCI)
The Virginia Stream Condition Index (VSCI) relies on eight metrics to categorize stream health According to Burton and Gerritsen (2003 p 1-1) this index ldquohellip can be used as a primary indicator of ecosystem health and can identify impairment with respect to the reference (or natural) conditionrdquo These metrics are numeric measures of a biological communityrsquos species composition diversity ecological niches or tolerance to pollution
Table 13 on the next page describes each VSCI metric lists its formula and provides the best standard value (BSV) for that metric Based on a 100-point scale the BSV is the score that reflects optimum conditions for aquatic life ie the best water quality value possible (DEQ 2006b pp 8 33) The table also explains whether the score increases or decreases with environmental stress
As Table 13 indicates each of the eight VSCI metrics is calculated individually Each formula provides a unitless score based on a 100-point scale with 0 as the worst possible score and 100 as the best Any metric that provides a result above 100 is changed to 100 in order for the index to work properly After the eight metrics are calculated they are summed and averaged to provide a final VSCI score on a 100-point scale
Based on statistical analysis of Virginiarsquos streams and their BMI communities the DEQ (2006b p 8) currently uses the following tiers to interpret VSCI scores
VSCI Scores Aquatic Life Use
(ALI) Tiers
lt 42 severely stressed
42-55 moderately stressed
55-63 unknownfair (gray zone)
63-73 good
gt73 excellent
Section 2 of this report presents the results of calculations for the six BMI sampling sites the research team studied in the Hiatt-Lick Run subwatershed in 2008
o
p
1 Total taxa the number of distinct taxa in the sample measures the overall diversity of the macroinvertebrate assemblage The best standard value (BSV) is 189 Score = 100 x (X189) Total taxa decreases with stress 2 EPT taxa the sum of the distinct taxa in the orders Ephemeroptera (mayflies) Plecoptera (stoneflies) and Trichoptera (caddisflies) These orders are known to be sensitive to pollution The best standard value (BSV) is 117 Score = 100 x (X117) EPT taxa decreases with stress 3 Percent Ephemeroptera the percent abundance of individuals in the sample that belong to the order Ephemeroptera (mayflies) The best standard value (BSV) is 477 Score = 100 x (X477) Percent Ephemeroptera decreases with stress 4 Percent Plecoptera + Trichoptera less Hydropsychidae the percent abundance of individuals in the sample that belong to the orders Plecoptera (stoneflies) and Trichoptera (caddisflies) excluding the pollution-tolerant caddisfly family Hydropsychidae The best standard value (BSV) is 562 Score = 100 x (X562) Percent Plecoptera + Trichoptera less Hydropsychidae decreases with stress 5 Percent Scrapers the percent abundance of individuals in the sample whose primary functional mechanism for feeding is grazing on the substrate or periphyton (ie attached algae and associated material) The best standard value (BSV) is 467 Score = 100 x (X467) Percent scrapers decreases with stress 6 Percent Chironomidae the percent abundance of individuals in the sample that are Chironomidae (non-biting midge) larvae and pupae The best standard value (BSV) is 16 Score = 100 x [(100-X)(100-16)] Percent Chironomidae increases with stress 7 Percent Top Two Dominant the percent abundance of the two most frequent taxa present in the sample The best standard value (BSV) is 328 Score = 100 x [(100-X)(100-328)] The Percent Top 2 Dominant increases with stress 8 Hilsenhoff Biotic Index (HBI) an abundance-weighted average pollution tolerance of all the individuals within a sample keyed to the family level Tolerance ratings range from 1 (highly intolerant) to 10 (highly tolerant) The best standard value (BSV) is 29 Score = 100 x [(10-X)(10-29)] The Hilsenhoff Biotic Index (HBI) increases with stress ______________________________________________________________________________ Table 13 Description of Benthic Macroinvertebrate VSCI Metrics Includes responses (increases decreases) of each VSCI metric to degraded water quality conditions Adapted from Burton and Gerritsen (2003 pp 3-15 3-16 6-2) and DEQ (2008)
q
Appendix C
LABORATORY BENCH SHEETS
Shenandoah Universityrsquos Environmental Studies Department developed the Benthic Macroinvertebrate Laboratory Bench Sheet and VSCI Worksheet to record BMI specimen identifications and their VSCI classifications for each sample
This form is based in part on bench sheets developed by the EPA and Virginia DEQ for recording specimen identifications Additional columns on Shenandoah Universityrsquos forms for relative numbers and VSCI classifications make the calculation and verification of VSCI scores easier
Note the column for taxonomistrsquos initials Specimens were identified by students in Shenandoah Universityrsquos research group and then verified or where necessary changed by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality This external review helps assure the validity of the study Originals of these bench sheets with handwritten taxonomistsrsquo initials will be kept on file at Shenandoah Universityrsquos Environmental Studies Department for five years after the completion of this study
r
s
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-1 STATION NAME Mouth LAT N 39deg 12749rsquo (upstream) LONG W 078deg 04209rsquo (upstream) LAT N 39deg 12729rsquo (downstream) LONG W 078deg 04148rsquo (downstream) FORM COMPILED BY Jeremy Tovar W S Bousquet RIVER BASIN Potomac River INVESTIGATORS Marie Dahl Brandon Millholand J W Pangle Sean Robertson Jeremy Tovar Woodward Bousquet Ben Bousquet
Collection date 21 May 2008 Sheet completion date 12 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Hirudinea 3 33 JT BVW PR 8 SP Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Coenagrionidae 2 22 BVW PR 9 CR Ephemeroptera (mayflies) Caenidae 1 11 JT BVW CG 6 SP Ephemerllidae 4 44 JT BVW CG 2 CR Heptageniidae 5 55 JT BVW SC 3 CG Isonychiidae 1 11 BVW CF 3 CG Plecoptera (stoneflies) Perlidae 14 154 JT BVW PR 2 CR Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Corydalidae 1 11 JT BVW PR 5 CR Coleoptera (beetles) Elmidae 24 264 I JT BVW SC 4 CG Dytiscidae 4 44 JT BVW PR 6 GN Psephenidae 3 33 JT BVW SC 4 CG Diptera (true flies) Tipulidae 3 33 JT BVW SH 4 BU Chironomidae 26 286 JT BVW CG 6 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
t
Other Total Individuals 91 1001 Total Taxa 13
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
u
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-1 STATION NAME Mouth LAT N 39deg 12749rsquo (upstream) LONG W 078deg 04209rsquo (upstream) LAT N 39deg 12729rsquo (downstream) LONG W 078deg 04148rsquo (downstream) FORM COMPILED BY Jeremy Tovar W S Bousquet RIVER BASIN Potomac River INVESTIGATORS Brandon Millholand JW Pangle Jeremy Tovar Heather Kepple
Collection date 10 October 2008 Sheet completion date 12 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Oligochaeta 3 35 JT BVW CG 8 BU Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Coenagrionidae 2 23 JT BVW PR 9 CR Ephemeroptera (mayflies) Heptageniidae 12 140 JT BVW SC 3 CG Isonychiidae 2 23 BVW CF 3 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Hydropsychidae 16 186 JT BVW CF 6 CG Philopotamidae 31 360 JT BVW CF 2 CG Hemiptera (true bugs) (Veliidae) (5) - JT BVW ( ) Megaloptera (dobsonflies etc) Corydalidae 10 116 JT BVW PR 5 CR Coleoptera (beetles) Dytiscidae 1 12 JT BVW PR 6 GN Psephenidae 7 81 JT BVW SC 4 CG Diptera (true flies) Tipulidae 2 23 BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
v
Other Total Individuals 86
(91) 999
Total Taxa 10 (11)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009 Veliidae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
w
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-2 STATION NAME Package Plant LAT N 39deg 12958rsquo (upstream) LONG W 078deg 04900 (upstream) LAT N 39deg 12958rsquo (downstream) LONG W 078deg 04822 (downstream) FORM COMPILED BY JW Pangle W Bousquet RIVER BASIN Potomac River INVESTIGATORS JW Pangle S Robertson J Tovar M Dahl B Millholland W Bousquet
Collection date 29 May 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Plecoptera (stoneflies) Perlidae 11 136 I JP BVW PR 1 CR Trichoptera (caddisflies) Hydropsychidae 10 123 I JP BVW CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 14 173 I JP BVW SC 4 CG Diptera (true flies) Chironomidae 32 395 I JP BVW CG 6 BU Simuliidae 14 173 I JP BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
x
Other Total Individuals 81 1000 Total Taxa 5
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-2 STATION NAME Package Plant LAT N 39deg 12958rsquo (upstream) LONG W 078deg 04900 (upstream) LAT N 39deg 12958rsquo (downstream) LONG W 078deg 04822 (downstream) FORM COMPILED BY JW Pangle W Bousquet RIVER BASIN Potomac River INVESTIGATORS JW Pangle Brandon Millholland Jeremy Tovar Heather Kepple
Collection date 10 October 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Heptageniidae 40 392 I WBJP SC 3 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Hydropsychidae 7 69 I JP WB BVW CF 6 CG Philopotamidae 28 275 I JP WB BVW CF 2 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Corydalidae 6 59 I JP WB BVW PR 5 CR Coleoptera (beetles) Psephenidae 7 69 I JP WB BVW SC 4 CG Elmidae 8 78 I JP WB BVW SC 4 CG Diptera (true flies) Tipulidae 6 59 I JP WB BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
y
z
Other Total Individuals 102 1001 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-3 STATION NAME Rt 664 Bridge LAT N 39deg 12934rsquo (upstream) LONG W 078deg 05019rsquo (upstream) LAT N 39deg 12951rsquo (downstream) LONG W 078deg 04958rsquo (downstream) FORM COMPILED BY S Robertson W Bousquet RIVER BASIN Potomac River INVESTIGATORS SRobertson BMillholland WBousquet MDahl JPangle
Collection date 28 May 2008 Sheet completion date 11 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Leptophlebiidae 1 15 I SGR BVW CG 2 CR Baetidae 1 15 I BVW CG 5 CG Plecoptera (stoneflies) Perlidae 3 46 I SGR BVW PR 1 CR Nemouridae 1 15 I BVW SH 3 CR Trichoptera (caddisflies) Hydropsychidae 5 77 I SGR BVW CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 27 415 I SGR BVW SC 4 CG Psephenidae 2 31 A SGR BVW SC 4 CG Diptera (true flies) Simuliidae 5 77 I SGR BVW CF 6 CG Chironomidae 20 308 I SGR BVW CG 6 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
aa
bb
Other Total Individuals 65 999 Total Taxa 9
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-3 STATION NAME Rt 664 Bridge LAT N 39deg 12934rsquo (upstream) LONG W 078deg 05019rsquo (upstream) LAT N 39deg 12951rsquo (downstream) LONG W 078deg 04958rsquo (downstream) FORM COMPILED BY S Robertson RIVER BASIN Potomac River INVESTIGATORS S Robertson M Dahl A Fisher H Kepple
Collection date 10 Oct 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Heptageniidae 2 20 I SGR SC 3 CG Isonychiidae 2 20 I BVW CF 2 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Philopotamidae 36 367 I SGR CF 2 CG Hydropsychidae 47 480 I SGR CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 2 20 A SGR SC 4 CG Psephenidae 8 82 A SGR SC 4 CG Diptera (true flies) Tabanidae 1 10 I SGR PR 7 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
cc
dd
Other Total Individuals 98 999 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-4 STATION NAME Unnamed Tributary LAT (upstream) N 39deg 13035rsquo LONG (upstream) W 078deg 05129rsquo LAT (downstream) N 39deg 13000rsquo LONG (downstream) W 078deg 5086rsquo FORM COMPILED BY A Fisher M Dahl rev W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson A Fisher H Kepple
Collection date 31 May 2008 Sheet completion date rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Flatworms (Turbellaria) Worms (Oligochaeta) Naididae 1 14 - BVW CG 8 BU Leeches (Hirudinea) Snails (Gastropoda) Clams (Bivalvia) Isopods (Isopoda) Asellidae 3 42 A AF BVW CG 8 SP Scuds (Amphipoda) Crayfish Shrimp (Decapoda) Water Mites (Hydracarina) Dragonflies (Odonata Anisoptera) Damselflies (Odonata Zygoptera) Mayflies (Ephemeroptera) Stoneflies (Plecoptera) Perlidae 2 28 I AF BVW PR 1 CR Caddisflies (Trichoptera) True Bugs (Hemiptera) Corixidae 1 14 I AF BVW MP 9 DV Dobsonflies (Megaloptera) Beetles (Coleoptera) Dytiscidae 1 14 I AF BVW PR 6 GN True Flies (Diptera) Chironomidae 61 847 I AF BVW CG 6 BU Simuliidae 3 42 I AF BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
ee
ff
Other Total Individuals 72 1001 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-4 STATION NAME Unnamed Tributary LAT (upstream) N 39deg 13035rsquo LONG (upstream) W 078deg 05129rsquo LAT (downstream) N 39deg 13000rsquo LONG (downstream) W 078deg 5086rsquo FORM COMPILED BY Andrew G M Fisher W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson A Fisher H Kepple
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Flatworms (Turbellaria) Worms (Oligochaeta) Lumbriculidae 2 32 A AF BVW CG 8 BU Leeches (Hirudinea) Snails (Gastropoda) Lymnaeidae 1 16 A BVW CG 9 GN Clams (Bivalvia) Isopods (Isopoda) Scuds (Amphipoda) Crayfish Shrimp (Decapoda) Cambaridae 1 16 A AF BVW GN 5 GN Water Mites (Hydracarina) Dragonflies (Odonata Anisoptera) Damselflies (Odonata Zygoptera) Calopterygidae 4 63 I BVW PR 7 CL Mayflies (Ephemeroptera) Tricorythidae 1 16 I BVW CG 5 SP Stoneflies (Plecoptera) Caddisflies (Trichoptera) Phryganeidae 4 63 I AF BVW SH 4 CR Hydropsychidae 6 95 I AF BVW CF 6 CG I True Bugs (Hemiptera) Corixidae 8 127 I AF BVW MP 9 DV (Veliidae ) (7) - I AF BVW ( ) Dobsonflies (Megaloptera) Sialidae 3 48 I AF BVW PR 7 SP Corydalidae 2 32 I AF BVW PR 5 CR Beetles (Coleoptera) Haliplidae 1 16 A AF BVW MP 8 GN Dysticidae 7 111 I AF BVW PR 6 GN Hydrophilidae 1 16 A AF BVW PR 8 GN Elmidae 3 48 I BVW SC 4 CG True Flies (Diptera) Chironomidae 8 127 I AF BVW CG 6 BU Simuliidae 7 111 I AF BVW CF 6 CG Tipulidae 4 63 I AF BVW SH 4 GU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
gg
hh
Other Total Individuals 63
(70) 1000
Total Taxa 17 (18)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Veliidae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-5 STATION NAME Lick Run LAT N 39deg 12752rsquo (upstream) LONG W 078deg 05685rsquo (upstream) LAT N 39deg 12784rsquo (downstream) LONG W 078deg 05631rsquo (downstream) FORM COMPILED BY Brandon Millholland W Bousquet RIVER BASIN Potomac River INVESTIGATORS J Pangle S Robertson W Bousquet JTovar M Dahl
Collection date 29 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Gomphidae 1 15 I BVW PR 3 BU Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Leptophlebiidae 11 167 A LBM BVW CG 2 CR Plecoptera (stoneflies) Perlidae 10 152 A LBM BVW PR 1 CR Perlodiae 1 15 A LBM BVW PR 2 CR Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 5 76 A LBM BVW SC 4 CG Elmidae 2 30 I LBM BVW SC 4 CG Diptera (true flies) (Culicidae) (3) - P LMB BVW ( ) Chironomidae 23 348 I LBM BVW CG 6 BU Simuliidae 13 197 I LBM BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
ii
jj
Other Total Individuals 66
(69) 1000
Total Taxa 7 (8)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Culicidae (Diptera) pupae not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl J Pangle S Robertson W Bousquet B Millholland J Tovar H Kepple
Collection date 30 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 1 12 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Asellidae 114 965 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Plecoptera (stoneflies) Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 2 23 A MRD BVW SC 4 CG Diptera (true flies)
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
kk
ll
Other Total Individuals 117 1000 Total Taxa 3
Notes Subsample taken 29 October 2009 All taxonomic determinations by MRD were confirmed by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson K McCann A Fisher H Kepple A Barrett
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 2 17 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Sphaeriidae 2 17 I MRD BVW CF 8 BU Isopoda (isopods) Asellidae 99 853 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Calopterygidae 2 17 A MRD BVW PR 7 CL Ephemeroptera (mayflies) Leptophlebiidae 2 17 I MRD BVW CG 2 CR Heptageniidae 4 34 A MRD BVW SC 3 CG Plecoptera (stoneflies) Perlidae 1 09 A MRD BVW PR 1 CR Trichoptera (caddisflies) Hemiptera (true bugs) (Gerridae) (1) - A MRD BVW ( ) Corixidae 1 09 A MRD BVW PR 5 CR Megaloptera (dobsonflies etc) Coleoptera (beetles) Dryopidae 1 09 A MRD BVW SC 5 CG Diptera (true flies) Tipulidae 2 17 I BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
mm
nn
Other Total Individuals 116
(117) 999
Total Taxa 10 (11)
Notes Subsample taken 6 November 2008 Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009 Gerridae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
- Disclaimer ii
- Title Page iii
- Acknowledgements v
- Table of Contents vii
- SECTION 2 RESULTS DATA TABLES 7
- OVERVIEW AND FINDINGS
- Introduction
- RESULTS DATA TABLES
- REFERENCES
- FIELD DATA SHEETS
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- SubstrateType
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- WATER QUALITY ANALYSIS
- THROUGH THE VIRGINIA
- STREAM CONDITION INDEX (VSCI)
- LABORATORY BENCH SHEETS
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
b
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Mouth STATION _HLR-1_____ STREAM CLASS LAT N 39 12749 (upstream) LONG W 078 04209(upstream) LAT N 39 12729 (downstream) LONG W 078 04148 (downstream) FORM COMPILED BY Jeremy Tovar RIVER BASIN Potomac River INVESTIGATORS JPangle B Millholland MDahl SRobertson W Bousquet JTovar BBousquet
DATE May 21 2008_________ TIME_250 PM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature __24___degC showers (intermittent) Comments _______________________
___ cloud cover _0_ ______________________________
X clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x ( Significant springs andor seeps) x (Valley ) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS
c
d
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence x FieldPasture Industrial Obvious sources Agricultural Other ____________ Stream scour steep slope erosion x Residential Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left Field and young thin deciduous forest 100 Bank=Grass cobble and shrub Right 90 maturing deciduous forest 45 shale slope Bank=Bedrock soil and herbs Indicate the dominant vegetation type(s) and record the dominant species
x Trees Shrubs x (Grasses) Forbs Dominant species Oak species and mix deciduous
INSTREAM FEATURES
Measured reach length ___100__m Canopy Cover Estimated stream width _35__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area __350_m2
Estimated stream depth _008_m Proportion of Reach Represented by Stream High water mark _15_m Morphology Types Surface velocity (at thalweg) _05__msec x Riffle__75___ x Run__20___ x Pool__5___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___00__ m2 Density of LWD _-_ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __15_____ x Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present Algae
WATER QUALITY Temperature __19___degC Water Odors x NormalNone Sewage Specific Conductance - Petroleum Chemical Fishy Other___________________ Dissolved Oxygen - Water Surface Oils Slick Sheen Globs Flecks pH __-_ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used _____NA________ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic x None Relict shells Other______None_____ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 8 Detritus sticks wood coarse plant materials
0 Boulder gt256 mm (10rsquorsquo) 0
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 51 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 40 Sand 006-2 mm (gritty) 1 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University
SITE CHARACTERIZATION FIELD DATA SHEET FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Package Plant STATION HLR-2 STREAM CLASS LAT N 39 129580 (upstream) LONG W 078 04900 (upstream) LAT N 39 129581 (downstream) LONG W 078 04822 (downstream) FORM COMPILED BY JW Pangle RIVER BASIN Potomac River INVESTIGATORS JPangle JTovar MDahl B Millholland SRobertson W Bousquet
DATE__19 May 2008_ TIME__1240__ PM
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature _____degC showers (intermittent) Comments __Sunny Day_____________
_5__ cloud cover _0__ ______________________________
x clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x (Significant springs andor seeps) x (Valley) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS
e
f
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence FieldPasture Industrial Obvious sources Agricultural Other ____________ __Upstream Construction and Stream bank Scour Residential __________________________________ Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left Deciduous forest with thick herb and shrub layers bank= vegetation and rocks Right Deciduous forest with thick herb and shrub layers bank=vegetation soil and rocks Indicate the dominant vegetation type(s) and record the dominant species
x Trees x Shrubs Grasses Forbs Dominant species Black Walnut White Ash Hackberry and American Sycamore
INSTREAM FEATURES
Measured reach length 100 m Canopy Cover Estimated stream width _35__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area __350_m2
Estimated stream depth __15__m Proportion of Reach Represented by Stream High water mark __22___m Morphology Types Surface velocity (at thalweg) _035__msec x Riffle__30___ x Run__30___ x Pool__40___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___10____ m2 Density of LWD _______ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __30___ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present__________________________________________________________
WATER QUALITY Temperature __18___degC Water Odors x NormalNone Sewage Specific Conductance __-____ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ____-____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used _____NA____ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other___None_____ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach SubstrateType Characteristic
Composition in Sampling Reach
Bedrock 3 Detritus sticks wood coarse plant materials
2 Boulder gt256 mm (10rsquorsquo) 5
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 70 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 22 Sand 006-2 mm (gritty) 0 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiattrsquos Run LOCATION Rt 664 Bridge STATION HLR-3 STREAM CLASS LAT N 39 12934 (upstream) LONG W 078 05019 (upstream) LAT N 39 12958 (downstream) LONG W 078 04958 (downstream) FORM COMPILED BY Sean Robertson RIVER BASIN Potomac River INVESTIGATORS SRobertson BMillholland WBousquet MDahl JPangle
DATE 28 May 2008 TIME 1127 AM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality 2008
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature __14___degC showers (intermittent) x Trace Comments _______________________
_65 x cloud cover ___ ______________________________
x clearsunny __________________________________
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x (Significant springs andor seeps) x (Valley) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS
g
h
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence Some evidence x FieldPasture Industrial x Obvious sources Agricultural x Other Old Package Plant Downstream x Residential Bridge and Road Power line and Pump station Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left 70 field 30 shrubs and deciduous trees Bank=Soil and vegetation Right Maturing deciduous forest steep slope Bank=Shale Bedrock Indicate the dominant vegetation type(s) and record the dominant species
x Trees x Shrubs x Grasses Forbs Dominant species Sycamore and Red Oaks
INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width __51___m Fully open x Partly open Partly shaded Fully shaded Sampling reach area _510_m2
Estimated stream depth __135___m Proportion of Reach Represented by Stream High water mark __-___m Morphology Types Surface velocity (at thalweg) __20___msec x Riffle_40_ x Run_50_ x Pool 10 Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___0____ m2 Density of LWD ___-____ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation ___50____ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present Brown Algae
WATER QUALITY Temperature __17___degC Water Odors x NormalNone Sewage Specific Conductance ___-___ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ___-_____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used ____NA_______ Clear x Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other____None____ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 15 Detritus sticks wood coarse plant materials
0 Boulder gt256 mm (10rsquorsquo) 15
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 62 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 8 Sand 006-2 mm (gritty) 0 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Unnamed Tributary STATION HLR-4 STREAM CLASS LAT N 39 13035 (upstream) LONG W 078 05129 (upstream) LAT N 39 13000 (downstream) LONG W 078 05086 (downstream) FORM COMPILED BY Marie Dahl RIVER BASIN Potomac River INVESTIGATORS M Dahl HKepple SRobertson AFisher
DATE 31 May 2008 TIME 5 PM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality 2008
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) YES x NO
rain (steady rain) Air Temperature __22___degC showers (intermittent) Comments _______________________
_0__ cloud cover _0__ ______________________________
x clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area __-___ km2 mi2 Montane Significant springs andor seeps x Valley Marsh or swamp Montane amp Valley Mixture of origins Other ___________________
COMMENTS Snowden Bridge development (upstream) and sewer line
i
j
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence x FieldPasture Industrial Obvious sources Agricultural Other ____________ Construction upstream and adjacent x Residential Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left Forested with rocky banks field across silt fence and 2m tree strip Right Forested with some rocky banks and forest through the highway Indicate the dominant vegetation type(s) and record the dominant species
x Trees x Shrubs x Grasses Forbs Dominant species White Ash and Hackberry
INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width ___38__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area __380__m2
Estimated stream depth __12__cm Proportion of Reach Represented by Stream High water mark __2___m Morphology Types Surface velocity (at thalweg) __46___msec x Riffle__25___ x Run__70___ x Pool__5___ Channelized Yes x No Dam Present x Yes No
LARGE WOODY DEBRIS
LWD _______ m2 Density of LWD _______ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation ___NA____ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present___Algae___
WATER QUALITY Temperature __16___degC Water Odors x NormalNone Sewage Specific Conductance __-____ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ___-_____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used ____NA_____ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells x Other___Shale_______ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 0 Detritus sticks wood coarse plant materials
10 Boulder gt256 mm (10rsquorsquo) 5
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 45 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 30 Sand 006-2 mm (gritty) 0 Marl grey shell
fragments 0
Silt 0004-006 mm 20 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Lick Run STATION HLR-5 STREAM CLASS LAT N 39 127517 (upstream) LONG W 078 05685(upstream) LAT N 39 12784 (downstream) LONG W 078 05631 (downstream) FORM COMPILED BY B Millholland RIVER BASIN Potomac River INVESTIGATORS B Millholland JTovar SRobertson MDahl WBousquet JPangle
DATE 29 May 2008 TIME 1040 AM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project
k
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature __15___degC showers (intermittent) Comments _______________________
_5_ X cloud cover _0__ ______________________________
x clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane Significant springs andor seeps x Valley Marsh or swamp Montane amp Valley Mixture of origins Other ___________________
COMMENTS Lick Run appears to be intermittent and may be dry in Fall 2008
l
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence x FieldPasture Industrial Obvious sources Agricultural Other ____________ Adjacent logging stream bank scour Residential Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left 85 young deciduous forest 15 logged Bank=Soil vegetation and cobbles Right Young deciduous forest on 30 slope Bank= Soil vegetation and cobble Indicate the dominant vegetation type(s) and record the dominant species
x Trees Shrubs Grasses Forbs Dominant species Mixed Deciduous INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width __11__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area 110 m2
Estimated stream depth _009__m Proportion of Reach Represented by Stream High water mark __96___m Morphology Types Surface velocity (at thalweg) __01___msec x Riffle__30___ x Run__5___ x Pool__65___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___0____ m2 Density of LWD ___-____ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __70___ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present__________________________________________________________
WATER QUALITY Temperature __14___degC Water Odors x NormalNone Sewage Specific Conductance __-__ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ___-___ Water Surface Oils Slick Sheen Globs Flecks pH __-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used _____NA______ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other_____None_____
SEDIMENT AND SUBSTRATE
Odors Deposits Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other________________ x Other Slightly Salty Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 0 Detritus sticks wood coarse plant materials
5 Boulder gt256 mm (10rsquorsquo) 0
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 75 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 23 Sand 006-2 mm (gritty) 2 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION McCann Farm STATION HLR-6 STREAM CLASS LAT N 39 13076 (upstream) LONG W 078 06539 (upstream) LAT N 39 13063 (downstream) LONG W 078 06473 (downstream) FORM COMPILED BY MDahl RIVER BASIN Potomac River INVESTIGATORS MDahl JPangle SRobertson W Bousqet BMillholland JTovar H Kepple
DATE 30 May 2008 TIME 1050 AM (start)
PROJECT NAME
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) YES x NO
rain (steady rain) Air Temperature __18___degC showers (intermittent) Comments _______________________
_85_x cloud cover _10__ ______________________________
clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x (Significant springs andor seeps) x ( Valley) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS Multi-habitat sampling with D-frame nets and seine
m
n
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial x No evidence Some evidence x FieldPasture Industrial Obvious sources x Agricultural Other ____________ Minor stream bank scour Residential Local Watershed Erosion x None Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left 35 bank grasses scattered trees and some bushes Right High bank with grasses and scattered trees Indicate the dominant vegetation type(s) and record the dominant species
x Trees Shrubs Grasses Forbs Dominant species Black Walnut and Festuca elatior
INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width __2___m Fully open Partly open x Partly shaded Fully shaded Sampling reach area __200_m2
Estimated stream depth __03_m Proportion of Reach Represented by Stream High water mark __1___m Morphology Types Surface velocity (at thalweg) __166_msec x Riffle__0___ x Run__55___ x Pool__45___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___2____ m2 Density of LWD ___-____ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __20_____ x Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present ____Glyceria (grass)sp__________
WATER QUALITY Temperature __16___degC Water Odors x NormalNone Sewage Specific Conductance ___-___ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ____-____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used ______NA___ Clear x Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other__NONE__ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 0 Detritus sticks wood coarse plant materials
5 Boulder gt256 mm (10rsquorsquo) 0
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 27 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 38 Sand 006-2 mm (gritty) 5 Marl grey shell
fragments 0 (lt1)
Silt 0004-006 mm 25 Clay lt 0004 mm (slick) 5 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Appendix B
WATER QUALITY ANALYSIS THROUGH THE VIRGINIA
STREAM CONDITION INDEX (VSCI)
The Virginia Stream Condition Index (VSCI) relies on eight metrics to categorize stream health According to Burton and Gerritsen (2003 p 1-1) this index ldquohellip can be used as a primary indicator of ecosystem health and can identify impairment with respect to the reference (or natural) conditionrdquo These metrics are numeric measures of a biological communityrsquos species composition diversity ecological niches or tolerance to pollution
Table 13 on the next page describes each VSCI metric lists its formula and provides the best standard value (BSV) for that metric Based on a 100-point scale the BSV is the score that reflects optimum conditions for aquatic life ie the best water quality value possible (DEQ 2006b pp 8 33) The table also explains whether the score increases or decreases with environmental stress
As Table 13 indicates each of the eight VSCI metrics is calculated individually Each formula provides a unitless score based on a 100-point scale with 0 as the worst possible score and 100 as the best Any metric that provides a result above 100 is changed to 100 in order for the index to work properly After the eight metrics are calculated they are summed and averaged to provide a final VSCI score on a 100-point scale
Based on statistical analysis of Virginiarsquos streams and their BMI communities the DEQ (2006b p 8) currently uses the following tiers to interpret VSCI scores
VSCI Scores Aquatic Life Use
(ALI) Tiers
lt 42 severely stressed
42-55 moderately stressed
55-63 unknownfair (gray zone)
63-73 good
gt73 excellent
Section 2 of this report presents the results of calculations for the six BMI sampling sites the research team studied in the Hiatt-Lick Run subwatershed in 2008
o
p
1 Total taxa the number of distinct taxa in the sample measures the overall diversity of the macroinvertebrate assemblage The best standard value (BSV) is 189 Score = 100 x (X189) Total taxa decreases with stress 2 EPT taxa the sum of the distinct taxa in the orders Ephemeroptera (mayflies) Plecoptera (stoneflies) and Trichoptera (caddisflies) These orders are known to be sensitive to pollution The best standard value (BSV) is 117 Score = 100 x (X117) EPT taxa decreases with stress 3 Percent Ephemeroptera the percent abundance of individuals in the sample that belong to the order Ephemeroptera (mayflies) The best standard value (BSV) is 477 Score = 100 x (X477) Percent Ephemeroptera decreases with stress 4 Percent Plecoptera + Trichoptera less Hydropsychidae the percent abundance of individuals in the sample that belong to the orders Plecoptera (stoneflies) and Trichoptera (caddisflies) excluding the pollution-tolerant caddisfly family Hydropsychidae The best standard value (BSV) is 562 Score = 100 x (X562) Percent Plecoptera + Trichoptera less Hydropsychidae decreases with stress 5 Percent Scrapers the percent abundance of individuals in the sample whose primary functional mechanism for feeding is grazing on the substrate or periphyton (ie attached algae and associated material) The best standard value (BSV) is 467 Score = 100 x (X467) Percent scrapers decreases with stress 6 Percent Chironomidae the percent abundance of individuals in the sample that are Chironomidae (non-biting midge) larvae and pupae The best standard value (BSV) is 16 Score = 100 x [(100-X)(100-16)] Percent Chironomidae increases with stress 7 Percent Top Two Dominant the percent abundance of the two most frequent taxa present in the sample The best standard value (BSV) is 328 Score = 100 x [(100-X)(100-328)] The Percent Top 2 Dominant increases with stress 8 Hilsenhoff Biotic Index (HBI) an abundance-weighted average pollution tolerance of all the individuals within a sample keyed to the family level Tolerance ratings range from 1 (highly intolerant) to 10 (highly tolerant) The best standard value (BSV) is 29 Score = 100 x [(10-X)(10-29)] The Hilsenhoff Biotic Index (HBI) increases with stress ______________________________________________________________________________ Table 13 Description of Benthic Macroinvertebrate VSCI Metrics Includes responses (increases decreases) of each VSCI metric to degraded water quality conditions Adapted from Burton and Gerritsen (2003 pp 3-15 3-16 6-2) and DEQ (2008)
q
Appendix C
LABORATORY BENCH SHEETS
Shenandoah Universityrsquos Environmental Studies Department developed the Benthic Macroinvertebrate Laboratory Bench Sheet and VSCI Worksheet to record BMI specimen identifications and their VSCI classifications for each sample
This form is based in part on bench sheets developed by the EPA and Virginia DEQ for recording specimen identifications Additional columns on Shenandoah Universityrsquos forms for relative numbers and VSCI classifications make the calculation and verification of VSCI scores easier
Note the column for taxonomistrsquos initials Specimens were identified by students in Shenandoah Universityrsquos research group and then verified or where necessary changed by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality This external review helps assure the validity of the study Originals of these bench sheets with handwritten taxonomistsrsquo initials will be kept on file at Shenandoah Universityrsquos Environmental Studies Department for five years after the completion of this study
r
s
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-1 STATION NAME Mouth LAT N 39deg 12749rsquo (upstream) LONG W 078deg 04209rsquo (upstream) LAT N 39deg 12729rsquo (downstream) LONG W 078deg 04148rsquo (downstream) FORM COMPILED BY Jeremy Tovar W S Bousquet RIVER BASIN Potomac River INVESTIGATORS Marie Dahl Brandon Millholand J W Pangle Sean Robertson Jeremy Tovar Woodward Bousquet Ben Bousquet
Collection date 21 May 2008 Sheet completion date 12 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Hirudinea 3 33 JT BVW PR 8 SP Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Coenagrionidae 2 22 BVW PR 9 CR Ephemeroptera (mayflies) Caenidae 1 11 JT BVW CG 6 SP Ephemerllidae 4 44 JT BVW CG 2 CR Heptageniidae 5 55 JT BVW SC 3 CG Isonychiidae 1 11 BVW CF 3 CG Plecoptera (stoneflies) Perlidae 14 154 JT BVW PR 2 CR Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Corydalidae 1 11 JT BVW PR 5 CR Coleoptera (beetles) Elmidae 24 264 I JT BVW SC 4 CG Dytiscidae 4 44 JT BVW PR 6 GN Psephenidae 3 33 JT BVW SC 4 CG Diptera (true flies) Tipulidae 3 33 JT BVW SH 4 BU Chironomidae 26 286 JT BVW CG 6 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
t
Other Total Individuals 91 1001 Total Taxa 13
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
u
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-1 STATION NAME Mouth LAT N 39deg 12749rsquo (upstream) LONG W 078deg 04209rsquo (upstream) LAT N 39deg 12729rsquo (downstream) LONG W 078deg 04148rsquo (downstream) FORM COMPILED BY Jeremy Tovar W S Bousquet RIVER BASIN Potomac River INVESTIGATORS Brandon Millholand JW Pangle Jeremy Tovar Heather Kepple
Collection date 10 October 2008 Sheet completion date 12 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Oligochaeta 3 35 JT BVW CG 8 BU Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Coenagrionidae 2 23 JT BVW PR 9 CR Ephemeroptera (mayflies) Heptageniidae 12 140 JT BVW SC 3 CG Isonychiidae 2 23 BVW CF 3 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Hydropsychidae 16 186 JT BVW CF 6 CG Philopotamidae 31 360 JT BVW CF 2 CG Hemiptera (true bugs) (Veliidae) (5) - JT BVW ( ) Megaloptera (dobsonflies etc) Corydalidae 10 116 JT BVW PR 5 CR Coleoptera (beetles) Dytiscidae 1 12 JT BVW PR 6 GN Psephenidae 7 81 JT BVW SC 4 CG Diptera (true flies) Tipulidae 2 23 BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
v
Other Total Individuals 86
(91) 999
Total Taxa 10 (11)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009 Veliidae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
w
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-2 STATION NAME Package Plant LAT N 39deg 12958rsquo (upstream) LONG W 078deg 04900 (upstream) LAT N 39deg 12958rsquo (downstream) LONG W 078deg 04822 (downstream) FORM COMPILED BY JW Pangle W Bousquet RIVER BASIN Potomac River INVESTIGATORS JW Pangle S Robertson J Tovar M Dahl B Millholland W Bousquet
Collection date 29 May 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Plecoptera (stoneflies) Perlidae 11 136 I JP BVW PR 1 CR Trichoptera (caddisflies) Hydropsychidae 10 123 I JP BVW CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 14 173 I JP BVW SC 4 CG Diptera (true flies) Chironomidae 32 395 I JP BVW CG 6 BU Simuliidae 14 173 I JP BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
x
Other Total Individuals 81 1000 Total Taxa 5
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-2 STATION NAME Package Plant LAT N 39deg 12958rsquo (upstream) LONG W 078deg 04900 (upstream) LAT N 39deg 12958rsquo (downstream) LONG W 078deg 04822 (downstream) FORM COMPILED BY JW Pangle W Bousquet RIVER BASIN Potomac River INVESTIGATORS JW Pangle Brandon Millholland Jeremy Tovar Heather Kepple
Collection date 10 October 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Heptageniidae 40 392 I WBJP SC 3 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Hydropsychidae 7 69 I JP WB BVW CF 6 CG Philopotamidae 28 275 I JP WB BVW CF 2 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Corydalidae 6 59 I JP WB BVW PR 5 CR Coleoptera (beetles) Psephenidae 7 69 I JP WB BVW SC 4 CG Elmidae 8 78 I JP WB BVW SC 4 CG Diptera (true flies) Tipulidae 6 59 I JP WB BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
y
z
Other Total Individuals 102 1001 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-3 STATION NAME Rt 664 Bridge LAT N 39deg 12934rsquo (upstream) LONG W 078deg 05019rsquo (upstream) LAT N 39deg 12951rsquo (downstream) LONG W 078deg 04958rsquo (downstream) FORM COMPILED BY S Robertson W Bousquet RIVER BASIN Potomac River INVESTIGATORS SRobertson BMillholland WBousquet MDahl JPangle
Collection date 28 May 2008 Sheet completion date 11 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Leptophlebiidae 1 15 I SGR BVW CG 2 CR Baetidae 1 15 I BVW CG 5 CG Plecoptera (stoneflies) Perlidae 3 46 I SGR BVW PR 1 CR Nemouridae 1 15 I BVW SH 3 CR Trichoptera (caddisflies) Hydropsychidae 5 77 I SGR BVW CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 27 415 I SGR BVW SC 4 CG Psephenidae 2 31 A SGR BVW SC 4 CG Diptera (true flies) Simuliidae 5 77 I SGR BVW CF 6 CG Chironomidae 20 308 I SGR BVW CG 6 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
aa
bb
Other Total Individuals 65 999 Total Taxa 9
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-3 STATION NAME Rt 664 Bridge LAT N 39deg 12934rsquo (upstream) LONG W 078deg 05019rsquo (upstream) LAT N 39deg 12951rsquo (downstream) LONG W 078deg 04958rsquo (downstream) FORM COMPILED BY S Robertson RIVER BASIN Potomac River INVESTIGATORS S Robertson M Dahl A Fisher H Kepple
Collection date 10 Oct 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Heptageniidae 2 20 I SGR SC 3 CG Isonychiidae 2 20 I BVW CF 2 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Philopotamidae 36 367 I SGR CF 2 CG Hydropsychidae 47 480 I SGR CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 2 20 A SGR SC 4 CG Psephenidae 8 82 A SGR SC 4 CG Diptera (true flies) Tabanidae 1 10 I SGR PR 7 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
cc
dd
Other Total Individuals 98 999 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-4 STATION NAME Unnamed Tributary LAT (upstream) N 39deg 13035rsquo LONG (upstream) W 078deg 05129rsquo LAT (downstream) N 39deg 13000rsquo LONG (downstream) W 078deg 5086rsquo FORM COMPILED BY A Fisher M Dahl rev W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson A Fisher H Kepple
Collection date 31 May 2008 Sheet completion date rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Flatworms (Turbellaria) Worms (Oligochaeta) Naididae 1 14 - BVW CG 8 BU Leeches (Hirudinea) Snails (Gastropoda) Clams (Bivalvia) Isopods (Isopoda) Asellidae 3 42 A AF BVW CG 8 SP Scuds (Amphipoda) Crayfish Shrimp (Decapoda) Water Mites (Hydracarina) Dragonflies (Odonata Anisoptera) Damselflies (Odonata Zygoptera) Mayflies (Ephemeroptera) Stoneflies (Plecoptera) Perlidae 2 28 I AF BVW PR 1 CR Caddisflies (Trichoptera) True Bugs (Hemiptera) Corixidae 1 14 I AF BVW MP 9 DV Dobsonflies (Megaloptera) Beetles (Coleoptera) Dytiscidae 1 14 I AF BVW PR 6 GN True Flies (Diptera) Chironomidae 61 847 I AF BVW CG 6 BU Simuliidae 3 42 I AF BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
ee
ff
Other Total Individuals 72 1001 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-4 STATION NAME Unnamed Tributary LAT (upstream) N 39deg 13035rsquo LONG (upstream) W 078deg 05129rsquo LAT (downstream) N 39deg 13000rsquo LONG (downstream) W 078deg 5086rsquo FORM COMPILED BY Andrew G M Fisher W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson A Fisher H Kepple
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Flatworms (Turbellaria) Worms (Oligochaeta) Lumbriculidae 2 32 A AF BVW CG 8 BU Leeches (Hirudinea) Snails (Gastropoda) Lymnaeidae 1 16 A BVW CG 9 GN Clams (Bivalvia) Isopods (Isopoda) Scuds (Amphipoda) Crayfish Shrimp (Decapoda) Cambaridae 1 16 A AF BVW GN 5 GN Water Mites (Hydracarina) Dragonflies (Odonata Anisoptera) Damselflies (Odonata Zygoptera) Calopterygidae 4 63 I BVW PR 7 CL Mayflies (Ephemeroptera) Tricorythidae 1 16 I BVW CG 5 SP Stoneflies (Plecoptera) Caddisflies (Trichoptera) Phryganeidae 4 63 I AF BVW SH 4 CR Hydropsychidae 6 95 I AF BVW CF 6 CG I True Bugs (Hemiptera) Corixidae 8 127 I AF BVW MP 9 DV (Veliidae ) (7) - I AF BVW ( ) Dobsonflies (Megaloptera) Sialidae 3 48 I AF BVW PR 7 SP Corydalidae 2 32 I AF BVW PR 5 CR Beetles (Coleoptera) Haliplidae 1 16 A AF BVW MP 8 GN Dysticidae 7 111 I AF BVW PR 6 GN Hydrophilidae 1 16 A AF BVW PR 8 GN Elmidae 3 48 I BVW SC 4 CG True Flies (Diptera) Chironomidae 8 127 I AF BVW CG 6 BU Simuliidae 7 111 I AF BVW CF 6 CG Tipulidae 4 63 I AF BVW SH 4 GU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
gg
hh
Other Total Individuals 63
(70) 1000
Total Taxa 17 (18)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Veliidae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-5 STATION NAME Lick Run LAT N 39deg 12752rsquo (upstream) LONG W 078deg 05685rsquo (upstream) LAT N 39deg 12784rsquo (downstream) LONG W 078deg 05631rsquo (downstream) FORM COMPILED BY Brandon Millholland W Bousquet RIVER BASIN Potomac River INVESTIGATORS J Pangle S Robertson W Bousquet JTovar M Dahl
Collection date 29 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Gomphidae 1 15 I BVW PR 3 BU Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Leptophlebiidae 11 167 A LBM BVW CG 2 CR Plecoptera (stoneflies) Perlidae 10 152 A LBM BVW PR 1 CR Perlodiae 1 15 A LBM BVW PR 2 CR Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 5 76 A LBM BVW SC 4 CG Elmidae 2 30 I LBM BVW SC 4 CG Diptera (true flies) (Culicidae) (3) - P LMB BVW ( ) Chironomidae 23 348 I LBM BVW CG 6 BU Simuliidae 13 197 I LBM BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
ii
jj
Other Total Individuals 66
(69) 1000
Total Taxa 7 (8)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Culicidae (Diptera) pupae not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl J Pangle S Robertson W Bousquet B Millholland J Tovar H Kepple
Collection date 30 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 1 12 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Asellidae 114 965 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Plecoptera (stoneflies) Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 2 23 A MRD BVW SC 4 CG Diptera (true flies)
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
kk
ll
Other Total Individuals 117 1000 Total Taxa 3
Notes Subsample taken 29 October 2009 All taxonomic determinations by MRD were confirmed by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson K McCann A Fisher H Kepple A Barrett
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 2 17 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Sphaeriidae 2 17 I MRD BVW CF 8 BU Isopoda (isopods) Asellidae 99 853 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Calopterygidae 2 17 A MRD BVW PR 7 CL Ephemeroptera (mayflies) Leptophlebiidae 2 17 I MRD BVW CG 2 CR Heptageniidae 4 34 A MRD BVW SC 3 CG Plecoptera (stoneflies) Perlidae 1 09 A MRD BVW PR 1 CR Trichoptera (caddisflies) Hemiptera (true bugs) (Gerridae) (1) - A MRD BVW ( ) Corixidae 1 09 A MRD BVW PR 5 CR Megaloptera (dobsonflies etc) Coleoptera (beetles) Dryopidae 1 09 A MRD BVW SC 5 CG Diptera (true flies) Tipulidae 2 17 I BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
mm
nn
Other Total Individuals 116
(117) 999
Total Taxa 10 (11)
Notes Subsample taken 6 November 2008 Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009 Gerridae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
- Disclaimer ii
- Title Page iii
- Acknowledgements v
- Table of Contents vii
- SECTION 2 RESULTS DATA TABLES 7
- OVERVIEW AND FINDINGS
- Introduction
- RESULTS DATA TABLES
- REFERENCES
- FIELD DATA SHEETS
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- SubstrateType
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- WATER QUALITY ANALYSIS
- THROUGH THE VIRGINIA
- STREAM CONDITION INDEX (VSCI)
- LABORATORY BENCH SHEETS
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Mouth STATION _HLR-1_____ STREAM CLASS LAT N 39 12749 (upstream) LONG W 078 04209(upstream) LAT N 39 12729 (downstream) LONG W 078 04148 (downstream) FORM COMPILED BY Jeremy Tovar RIVER BASIN Potomac River INVESTIGATORS JPangle B Millholland MDahl SRobertson W Bousquet JTovar BBousquet
DATE May 21 2008_________ TIME_250 PM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature __24___degC showers (intermittent) Comments _______________________
___ cloud cover _0_ ______________________________
X clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x ( Significant springs andor seeps) x (Valley ) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS
c
d
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence x FieldPasture Industrial Obvious sources Agricultural Other ____________ Stream scour steep slope erosion x Residential Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left Field and young thin deciduous forest 100 Bank=Grass cobble and shrub Right 90 maturing deciduous forest 45 shale slope Bank=Bedrock soil and herbs Indicate the dominant vegetation type(s) and record the dominant species
x Trees Shrubs x (Grasses) Forbs Dominant species Oak species and mix deciduous
INSTREAM FEATURES
Measured reach length ___100__m Canopy Cover Estimated stream width _35__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area __350_m2
Estimated stream depth _008_m Proportion of Reach Represented by Stream High water mark _15_m Morphology Types Surface velocity (at thalweg) _05__msec x Riffle__75___ x Run__20___ x Pool__5___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___00__ m2 Density of LWD _-_ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __15_____ x Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present Algae
WATER QUALITY Temperature __19___degC Water Odors x NormalNone Sewage Specific Conductance - Petroleum Chemical Fishy Other___________________ Dissolved Oxygen - Water Surface Oils Slick Sheen Globs Flecks pH __-_ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used _____NA________ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic x None Relict shells Other______None_____ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 8 Detritus sticks wood coarse plant materials
0 Boulder gt256 mm (10rsquorsquo) 0
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 51 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 40 Sand 006-2 mm (gritty) 1 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University
SITE CHARACTERIZATION FIELD DATA SHEET FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Package Plant STATION HLR-2 STREAM CLASS LAT N 39 129580 (upstream) LONG W 078 04900 (upstream) LAT N 39 129581 (downstream) LONG W 078 04822 (downstream) FORM COMPILED BY JW Pangle RIVER BASIN Potomac River INVESTIGATORS JPangle JTovar MDahl B Millholland SRobertson W Bousquet
DATE__19 May 2008_ TIME__1240__ PM
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature _____degC showers (intermittent) Comments __Sunny Day_____________
_5__ cloud cover _0__ ______________________________
x clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x (Significant springs andor seeps) x (Valley) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS
e
f
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence FieldPasture Industrial Obvious sources Agricultural Other ____________ __Upstream Construction and Stream bank Scour Residential __________________________________ Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left Deciduous forest with thick herb and shrub layers bank= vegetation and rocks Right Deciduous forest with thick herb and shrub layers bank=vegetation soil and rocks Indicate the dominant vegetation type(s) and record the dominant species
x Trees x Shrubs Grasses Forbs Dominant species Black Walnut White Ash Hackberry and American Sycamore
INSTREAM FEATURES
Measured reach length 100 m Canopy Cover Estimated stream width _35__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area __350_m2
Estimated stream depth __15__m Proportion of Reach Represented by Stream High water mark __22___m Morphology Types Surface velocity (at thalweg) _035__msec x Riffle__30___ x Run__30___ x Pool__40___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___10____ m2 Density of LWD _______ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __30___ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present__________________________________________________________
WATER QUALITY Temperature __18___degC Water Odors x NormalNone Sewage Specific Conductance __-____ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ____-____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used _____NA____ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other___None_____ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach SubstrateType Characteristic
Composition in Sampling Reach
Bedrock 3 Detritus sticks wood coarse plant materials
2 Boulder gt256 mm (10rsquorsquo) 5
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 70 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 22 Sand 006-2 mm (gritty) 0 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiattrsquos Run LOCATION Rt 664 Bridge STATION HLR-3 STREAM CLASS LAT N 39 12934 (upstream) LONG W 078 05019 (upstream) LAT N 39 12958 (downstream) LONG W 078 04958 (downstream) FORM COMPILED BY Sean Robertson RIVER BASIN Potomac River INVESTIGATORS SRobertson BMillholland WBousquet MDahl JPangle
DATE 28 May 2008 TIME 1127 AM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality 2008
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature __14___degC showers (intermittent) x Trace Comments _______________________
_65 x cloud cover ___ ______________________________
x clearsunny __________________________________
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x (Significant springs andor seeps) x (Valley) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS
g
h
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence Some evidence x FieldPasture Industrial x Obvious sources Agricultural x Other Old Package Plant Downstream x Residential Bridge and Road Power line and Pump station Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left 70 field 30 shrubs and deciduous trees Bank=Soil and vegetation Right Maturing deciduous forest steep slope Bank=Shale Bedrock Indicate the dominant vegetation type(s) and record the dominant species
x Trees x Shrubs x Grasses Forbs Dominant species Sycamore and Red Oaks
INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width __51___m Fully open x Partly open Partly shaded Fully shaded Sampling reach area _510_m2
Estimated stream depth __135___m Proportion of Reach Represented by Stream High water mark __-___m Morphology Types Surface velocity (at thalweg) __20___msec x Riffle_40_ x Run_50_ x Pool 10 Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___0____ m2 Density of LWD ___-____ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation ___50____ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present Brown Algae
WATER QUALITY Temperature __17___degC Water Odors x NormalNone Sewage Specific Conductance ___-___ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ___-_____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used ____NA_______ Clear x Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other____None____ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 15 Detritus sticks wood coarse plant materials
0 Boulder gt256 mm (10rsquorsquo) 15
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 62 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 8 Sand 006-2 mm (gritty) 0 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Unnamed Tributary STATION HLR-4 STREAM CLASS LAT N 39 13035 (upstream) LONG W 078 05129 (upstream) LAT N 39 13000 (downstream) LONG W 078 05086 (downstream) FORM COMPILED BY Marie Dahl RIVER BASIN Potomac River INVESTIGATORS M Dahl HKepple SRobertson AFisher
DATE 31 May 2008 TIME 5 PM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality 2008
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) YES x NO
rain (steady rain) Air Temperature __22___degC showers (intermittent) Comments _______________________
_0__ cloud cover _0__ ______________________________
x clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area __-___ km2 mi2 Montane Significant springs andor seeps x Valley Marsh or swamp Montane amp Valley Mixture of origins Other ___________________
COMMENTS Snowden Bridge development (upstream) and sewer line
i
j
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence x FieldPasture Industrial Obvious sources Agricultural Other ____________ Construction upstream and adjacent x Residential Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left Forested with rocky banks field across silt fence and 2m tree strip Right Forested with some rocky banks and forest through the highway Indicate the dominant vegetation type(s) and record the dominant species
x Trees x Shrubs x Grasses Forbs Dominant species White Ash and Hackberry
INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width ___38__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area __380__m2
Estimated stream depth __12__cm Proportion of Reach Represented by Stream High water mark __2___m Morphology Types Surface velocity (at thalweg) __46___msec x Riffle__25___ x Run__70___ x Pool__5___ Channelized Yes x No Dam Present x Yes No
LARGE WOODY DEBRIS
LWD _______ m2 Density of LWD _______ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation ___NA____ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present___Algae___
WATER QUALITY Temperature __16___degC Water Odors x NormalNone Sewage Specific Conductance __-____ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ___-_____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used ____NA_____ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells x Other___Shale_______ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 0 Detritus sticks wood coarse plant materials
10 Boulder gt256 mm (10rsquorsquo) 5
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 45 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 30 Sand 006-2 mm (gritty) 0 Marl grey shell
fragments 0
Silt 0004-006 mm 20 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Lick Run STATION HLR-5 STREAM CLASS LAT N 39 127517 (upstream) LONG W 078 05685(upstream) LAT N 39 12784 (downstream) LONG W 078 05631 (downstream) FORM COMPILED BY B Millholland RIVER BASIN Potomac River INVESTIGATORS B Millholland JTovar SRobertson MDahl WBousquet JPangle
DATE 29 May 2008 TIME 1040 AM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project
k
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature __15___degC showers (intermittent) Comments _______________________
_5_ X cloud cover _0__ ______________________________
x clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane Significant springs andor seeps x Valley Marsh or swamp Montane amp Valley Mixture of origins Other ___________________
COMMENTS Lick Run appears to be intermittent and may be dry in Fall 2008
l
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence x FieldPasture Industrial Obvious sources Agricultural Other ____________ Adjacent logging stream bank scour Residential Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left 85 young deciduous forest 15 logged Bank=Soil vegetation and cobbles Right Young deciduous forest on 30 slope Bank= Soil vegetation and cobble Indicate the dominant vegetation type(s) and record the dominant species
x Trees Shrubs Grasses Forbs Dominant species Mixed Deciduous INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width __11__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area 110 m2
Estimated stream depth _009__m Proportion of Reach Represented by Stream High water mark __96___m Morphology Types Surface velocity (at thalweg) __01___msec x Riffle__30___ x Run__5___ x Pool__65___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___0____ m2 Density of LWD ___-____ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __70___ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present__________________________________________________________
WATER QUALITY Temperature __14___degC Water Odors x NormalNone Sewage Specific Conductance __-__ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ___-___ Water Surface Oils Slick Sheen Globs Flecks pH __-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used _____NA______ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other_____None_____
SEDIMENT AND SUBSTRATE
Odors Deposits Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other________________ x Other Slightly Salty Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 0 Detritus sticks wood coarse plant materials
5 Boulder gt256 mm (10rsquorsquo) 0
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 75 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 23 Sand 006-2 mm (gritty) 2 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION McCann Farm STATION HLR-6 STREAM CLASS LAT N 39 13076 (upstream) LONG W 078 06539 (upstream) LAT N 39 13063 (downstream) LONG W 078 06473 (downstream) FORM COMPILED BY MDahl RIVER BASIN Potomac River INVESTIGATORS MDahl JPangle SRobertson W Bousqet BMillholland JTovar H Kepple
DATE 30 May 2008 TIME 1050 AM (start)
PROJECT NAME
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) YES x NO
rain (steady rain) Air Temperature __18___degC showers (intermittent) Comments _______________________
_85_x cloud cover _10__ ______________________________
clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x (Significant springs andor seeps) x ( Valley) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS Multi-habitat sampling with D-frame nets and seine
m
n
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial x No evidence Some evidence x FieldPasture Industrial Obvious sources x Agricultural Other ____________ Minor stream bank scour Residential Local Watershed Erosion x None Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left 35 bank grasses scattered trees and some bushes Right High bank with grasses and scattered trees Indicate the dominant vegetation type(s) and record the dominant species
x Trees Shrubs Grasses Forbs Dominant species Black Walnut and Festuca elatior
INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width __2___m Fully open Partly open x Partly shaded Fully shaded Sampling reach area __200_m2
Estimated stream depth __03_m Proportion of Reach Represented by Stream High water mark __1___m Morphology Types Surface velocity (at thalweg) __166_msec x Riffle__0___ x Run__55___ x Pool__45___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___2____ m2 Density of LWD ___-____ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __20_____ x Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present ____Glyceria (grass)sp__________
WATER QUALITY Temperature __16___degC Water Odors x NormalNone Sewage Specific Conductance ___-___ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ____-____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used ______NA___ Clear x Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other__NONE__ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 0 Detritus sticks wood coarse plant materials
5 Boulder gt256 mm (10rsquorsquo) 0
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 27 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 38 Sand 006-2 mm (gritty) 5 Marl grey shell
fragments 0 (lt1)
Silt 0004-006 mm 25 Clay lt 0004 mm (slick) 5 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Appendix B
WATER QUALITY ANALYSIS THROUGH THE VIRGINIA
STREAM CONDITION INDEX (VSCI)
The Virginia Stream Condition Index (VSCI) relies on eight metrics to categorize stream health According to Burton and Gerritsen (2003 p 1-1) this index ldquohellip can be used as a primary indicator of ecosystem health and can identify impairment with respect to the reference (or natural) conditionrdquo These metrics are numeric measures of a biological communityrsquos species composition diversity ecological niches or tolerance to pollution
Table 13 on the next page describes each VSCI metric lists its formula and provides the best standard value (BSV) for that metric Based on a 100-point scale the BSV is the score that reflects optimum conditions for aquatic life ie the best water quality value possible (DEQ 2006b pp 8 33) The table also explains whether the score increases or decreases with environmental stress
As Table 13 indicates each of the eight VSCI metrics is calculated individually Each formula provides a unitless score based on a 100-point scale with 0 as the worst possible score and 100 as the best Any metric that provides a result above 100 is changed to 100 in order for the index to work properly After the eight metrics are calculated they are summed and averaged to provide a final VSCI score on a 100-point scale
Based on statistical analysis of Virginiarsquos streams and their BMI communities the DEQ (2006b p 8) currently uses the following tiers to interpret VSCI scores
VSCI Scores Aquatic Life Use
(ALI) Tiers
lt 42 severely stressed
42-55 moderately stressed
55-63 unknownfair (gray zone)
63-73 good
gt73 excellent
Section 2 of this report presents the results of calculations for the six BMI sampling sites the research team studied in the Hiatt-Lick Run subwatershed in 2008
o
p
1 Total taxa the number of distinct taxa in the sample measures the overall diversity of the macroinvertebrate assemblage The best standard value (BSV) is 189 Score = 100 x (X189) Total taxa decreases with stress 2 EPT taxa the sum of the distinct taxa in the orders Ephemeroptera (mayflies) Plecoptera (stoneflies) and Trichoptera (caddisflies) These orders are known to be sensitive to pollution The best standard value (BSV) is 117 Score = 100 x (X117) EPT taxa decreases with stress 3 Percent Ephemeroptera the percent abundance of individuals in the sample that belong to the order Ephemeroptera (mayflies) The best standard value (BSV) is 477 Score = 100 x (X477) Percent Ephemeroptera decreases with stress 4 Percent Plecoptera + Trichoptera less Hydropsychidae the percent abundance of individuals in the sample that belong to the orders Plecoptera (stoneflies) and Trichoptera (caddisflies) excluding the pollution-tolerant caddisfly family Hydropsychidae The best standard value (BSV) is 562 Score = 100 x (X562) Percent Plecoptera + Trichoptera less Hydropsychidae decreases with stress 5 Percent Scrapers the percent abundance of individuals in the sample whose primary functional mechanism for feeding is grazing on the substrate or periphyton (ie attached algae and associated material) The best standard value (BSV) is 467 Score = 100 x (X467) Percent scrapers decreases with stress 6 Percent Chironomidae the percent abundance of individuals in the sample that are Chironomidae (non-biting midge) larvae and pupae The best standard value (BSV) is 16 Score = 100 x [(100-X)(100-16)] Percent Chironomidae increases with stress 7 Percent Top Two Dominant the percent abundance of the two most frequent taxa present in the sample The best standard value (BSV) is 328 Score = 100 x [(100-X)(100-328)] The Percent Top 2 Dominant increases with stress 8 Hilsenhoff Biotic Index (HBI) an abundance-weighted average pollution tolerance of all the individuals within a sample keyed to the family level Tolerance ratings range from 1 (highly intolerant) to 10 (highly tolerant) The best standard value (BSV) is 29 Score = 100 x [(10-X)(10-29)] The Hilsenhoff Biotic Index (HBI) increases with stress ______________________________________________________________________________ Table 13 Description of Benthic Macroinvertebrate VSCI Metrics Includes responses (increases decreases) of each VSCI metric to degraded water quality conditions Adapted from Burton and Gerritsen (2003 pp 3-15 3-16 6-2) and DEQ (2008)
q
Appendix C
LABORATORY BENCH SHEETS
Shenandoah Universityrsquos Environmental Studies Department developed the Benthic Macroinvertebrate Laboratory Bench Sheet and VSCI Worksheet to record BMI specimen identifications and their VSCI classifications for each sample
This form is based in part on bench sheets developed by the EPA and Virginia DEQ for recording specimen identifications Additional columns on Shenandoah Universityrsquos forms for relative numbers and VSCI classifications make the calculation and verification of VSCI scores easier
Note the column for taxonomistrsquos initials Specimens were identified by students in Shenandoah Universityrsquos research group and then verified or where necessary changed by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality This external review helps assure the validity of the study Originals of these bench sheets with handwritten taxonomistsrsquo initials will be kept on file at Shenandoah Universityrsquos Environmental Studies Department for five years after the completion of this study
r
s
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-1 STATION NAME Mouth LAT N 39deg 12749rsquo (upstream) LONG W 078deg 04209rsquo (upstream) LAT N 39deg 12729rsquo (downstream) LONG W 078deg 04148rsquo (downstream) FORM COMPILED BY Jeremy Tovar W S Bousquet RIVER BASIN Potomac River INVESTIGATORS Marie Dahl Brandon Millholand J W Pangle Sean Robertson Jeremy Tovar Woodward Bousquet Ben Bousquet
Collection date 21 May 2008 Sheet completion date 12 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Hirudinea 3 33 JT BVW PR 8 SP Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Coenagrionidae 2 22 BVW PR 9 CR Ephemeroptera (mayflies) Caenidae 1 11 JT BVW CG 6 SP Ephemerllidae 4 44 JT BVW CG 2 CR Heptageniidae 5 55 JT BVW SC 3 CG Isonychiidae 1 11 BVW CF 3 CG Plecoptera (stoneflies) Perlidae 14 154 JT BVW PR 2 CR Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Corydalidae 1 11 JT BVW PR 5 CR Coleoptera (beetles) Elmidae 24 264 I JT BVW SC 4 CG Dytiscidae 4 44 JT BVW PR 6 GN Psephenidae 3 33 JT BVW SC 4 CG Diptera (true flies) Tipulidae 3 33 JT BVW SH 4 BU Chironomidae 26 286 JT BVW CG 6 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
t
Other Total Individuals 91 1001 Total Taxa 13
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
u
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-1 STATION NAME Mouth LAT N 39deg 12749rsquo (upstream) LONG W 078deg 04209rsquo (upstream) LAT N 39deg 12729rsquo (downstream) LONG W 078deg 04148rsquo (downstream) FORM COMPILED BY Jeremy Tovar W S Bousquet RIVER BASIN Potomac River INVESTIGATORS Brandon Millholand JW Pangle Jeremy Tovar Heather Kepple
Collection date 10 October 2008 Sheet completion date 12 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Oligochaeta 3 35 JT BVW CG 8 BU Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Coenagrionidae 2 23 JT BVW PR 9 CR Ephemeroptera (mayflies) Heptageniidae 12 140 JT BVW SC 3 CG Isonychiidae 2 23 BVW CF 3 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Hydropsychidae 16 186 JT BVW CF 6 CG Philopotamidae 31 360 JT BVW CF 2 CG Hemiptera (true bugs) (Veliidae) (5) - JT BVW ( ) Megaloptera (dobsonflies etc) Corydalidae 10 116 JT BVW PR 5 CR Coleoptera (beetles) Dytiscidae 1 12 JT BVW PR 6 GN Psephenidae 7 81 JT BVW SC 4 CG Diptera (true flies) Tipulidae 2 23 BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
v
Other Total Individuals 86
(91) 999
Total Taxa 10 (11)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009 Veliidae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
w
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-2 STATION NAME Package Plant LAT N 39deg 12958rsquo (upstream) LONG W 078deg 04900 (upstream) LAT N 39deg 12958rsquo (downstream) LONG W 078deg 04822 (downstream) FORM COMPILED BY JW Pangle W Bousquet RIVER BASIN Potomac River INVESTIGATORS JW Pangle S Robertson J Tovar M Dahl B Millholland W Bousquet
Collection date 29 May 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Plecoptera (stoneflies) Perlidae 11 136 I JP BVW PR 1 CR Trichoptera (caddisflies) Hydropsychidae 10 123 I JP BVW CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 14 173 I JP BVW SC 4 CG Diptera (true flies) Chironomidae 32 395 I JP BVW CG 6 BU Simuliidae 14 173 I JP BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
x
Other Total Individuals 81 1000 Total Taxa 5
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-2 STATION NAME Package Plant LAT N 39deg 12958rsquo (upstream) LONG W 078deg 04900 (upstream) LAT N 39deg 12958rsquo (downstream) LONG W 078deg 04822 (downstream) FORM COMPILED BY JW Pangle W Bousquet RIVER BASIN Potomac River INVESTIGATORS JW Pangle Brandon Millholland Jeremy Tovar Heather Kepple
Collection date 10 October 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Heptageniidae 40 392 I WBJP SC 3 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Hydropsychidae 7 69 I JP WB BVW CF 6 CG Philopotamidae 28 275 I JP WB BVW CF 2 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Corydalidae 6 59 I JP WB BVW PR 5 CR Coleoptera (beetles) Psephenidae 7 69 I JP WB BVW SC 4 CG Elmidae 8 78 I JP WB BVW SC 4 CG Diptera (true flies) Tipulidae 6 59 I JP WB BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
y
z
Other Total Individuals 102 1001 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-3 STATION NAME Rt 664 Bridge LAT N 39deg 12934rsquo (upstream) LONG W 078deg 05019rsquo (upstream) LAT N 39deg 12951rsquo (downstream) LONG W 078deg 04958rsquo (downstream) FORM COMPILED BY S Robertson W Bousquet RIVER BASIN Potomac River INVESTIGATORS SRobertson BMillholland WBousquet MDahl JPangle
Collection date 28 May 2008 Sheet completion date 11 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Leptophlebiidae 1 15 I SGR BVW CG 2 CR Baetidae 1 15 I BVW CG 5 CG Plecoptera (stoneflies) Perlidae 3 46 I SGR BVW PR 1 CR Nemouridae 1 15 I BVW SH 3 CR Trichoptera (caddisflies) Hydropsychidae 5 77 I SGR BVW CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 27 415 I SGR BVW SC 4 CG Psephenidae 2 31 A SGR BVW SC 4 CG Diptera (true flies) Simuliidae 5 77 I SGR BVW CF 6 CG Chironomidae 20 308 I SGR BVW CG 6 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
aa
bb
Other Total Individuals 65 999 Total Taxa 9
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-3 STATION NAME Rt 664 Bridge LAT N 39deg 12934rsquo (upstream) LONG W 078deg 05019rsquo (upstream) LAT N 39deg 12951rsquo (downstream) LONG W 078deg 04958rsquo (downstream) FORM COMPILED BY S Robertson RIVER BASIN Potomac River INVESTIGATORS S Robertson M Dahl A Fisher H Kepple
Collection date 10 Oct 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Heptageniidae 2 20 I SGR SC 3 CG Isonychiidae 2 20 I BVW CF 2 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Philopotamidae 36 367 I SGR CF 2 CG Hydropsychidae 47 480 I SGR CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 2 20 A SGR SC 4 CG Psephenidae 8 82 A SGR SC 4 CG Diptera (true flies) Tabanidae 1 10 I SGR PR 7 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
cc
dd
Other Total Individuals 98 999 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-4 STATION NAME Unnamed Tributary LAT (upstream) N 39deg 13035rsquo LONG (upstream) W 078deg 05129rsquo LAT (downstream) N 39deg 13000rsquo LONG (downstream) W 078deg 5086rsquo FORM COMPILED BY A Fisher M Dahl rev W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson A Fisher H Kepple
Collection date 31 May 2008 Sheet completion date rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Flatworms (Turbellaria) Worms (Oligochaeta) Naididae 1 14 - BVW CG 8 BU Leeches (Hirudinea) Snails (Gastropoda) Clams (Bivalvia) Isopods (Isopoda) Asellidae 3 42 A AF BVW CG 8 SP Scuds (Amphipoda) Crayfish Shrimp (Decapoda) Water Mites (Hydracarina) Dragonflies (Odonata Anisoptera) Damselflies (Odonata Zygoptera) Mayflies (Ephemeroptera) Stoneflies (Plecoptera) Perlidae 2 28 I AF BVW PR 1 CR Caddisflies (Trichoptera) True Bugs (Hemiptera) Corixidae 1 14 I AF BVW MP 9 DV Dobsonflies (Megaloptera) Beetles (Coleoptera) Dytiscidae 1 14 I AF BVW PR 6 GN True Flies (Diptera) Chironomidae 61 847 I AF BVW CG 6 BU Simuliidae 3 42 I AF BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
ee
ff
Other Total Individuals 72 1001 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-4 STATION NAME Unnamed Tributary LAT (upstream) N 39deg 13035rsquo LONG (upstream) W 078deg 05129rsquo LAT (downstream) N 39deg 13000rsquo LONG (downstream) W 078deg 5086rsquo FORM COMPILED BY Andrew G M Fisher W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson A Fisher H Kepple
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Flatworms (Turbellaria) Worms (Oligochaeta) Lumbriculidae 2 32 A AF BVW CG 8 BU Leeches (Hirudinea) Snails (Gastropoda) Lymnaeidae 1 16 A BVW CG 9 GN Clams (Bivalvia) Isopods (Isopoda) Scuds (Amphipoda) Crayfish Shrimp (Decapoda) Cambaridae 1 16 A AF BVW GN 5 GN Water Mites (Hydracarina) Dragonflies (Odonata Anisoptera) Damselflies (Odonata Zygoptera) Calopterygidae 4 63 I BVW PR 7 CL Mayflies (Ephemeroptera) Tricorythidae 1 16 I BVW CG 5 SP Stoneflies (Plecoptera) Caddisflies (Trichoptera) Phryganeidae 4 63 I AF BVW SH 4 CR Hydropsychidae 6 95 I AF BVW CF 6 CG I True Bugs (Hemiptera) Corixidae 8 127 I AF BVW MP 9 DV (Veliidae ) (7) - I AF BVW ( ) Dobsonflies (Megaloptera) Sialidae 3 48 I AF BVW PR 7 SP Corydalidae 2 32 I AF BVW PR 5 CR Beetles (Coleoptera) Haliplidae 1 16 A AF BVW MP 8 GN Dysticidae 7 111 I AF BVW PR 6 GN Hydrophilidae 1 16 A AF BVW PR 8 GN Elmidae 3 48 I BVW SC 4 CG True Flies (Diptera) Chironomidae 8 127 I AF BVW CG 6 BU Simuliidae 7 111 I AF BVW CF 6 CG Tipulidae 4 63 I AF BVW SH 4 GU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
gg
hh
Other Total Individuals 63
(70) 1000
Total Taxa 17 (18)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Veliidae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-5 STATION NAME Lick Run LAT N 39deg 12752rsquo (upstream) LONG W 078deg 05685rsquo (upstream) LAT N 39deg 12784rsquo (downstream) LONG W 078deg 05631rsquo (downstream) FORM COMPILED BY Brandon Millholland W Bousquet RIVER BASIN Potomac River INVESTIGATORS J Pangle S Robertson W Bousquet JTovar M Dahl
Collection date 29 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Gomphidae 1 15 I BVW PR 3 BU Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Leptophlebiidae 11 167 A LBM BVW CG 2 CR Plecoptera (stoneflies) Perlidae 10 152 A LBM BVW PR 1 CR Perlodiae 1 15 A LBM BVW PR 2 CR Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 5 76 A LBM BVW SC 4 CG Elmidae 2 30 I LBM BVW SC 4 CG Diptera (true flies) (Culicidae) (3) - P LMB BVW ( ) Chironomidae 23 348 I LBM BVW CG 6 BU Simuliidae 13 197 I LBM BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
ii
jj
Other Total Individuals 66
(69) 1000
Total Taxa 7 (8)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Culicidae (Diptera) pupae not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl J Pangle S Robertson W Bousquet B Millholland J Tovar H Kepple
Collection date 30 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 1 12 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Asellidae 114 965 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Plecoptera (stoneflies) Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 2 23 A MRD BVW SC 4 CG Diptera (true flies)
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
kk
ll
Other Total Individuals 117 1000 Total Taxa 3
Notes Subsample taken 29 October 2009 All taxonomic determinations by MRD were confirmed by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson K McCann A Fisher H Kepple A Barrett
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 2 17 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Sphaeriidae 2 17 I MRD BVW CF 8 BU Isopoda (isopods) Asellidae 99 853 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Calopterygidae 2 17 A MRD BVW PR 7 CL Ephemeroptera (mayflies) Leptophlebiidae 2 17 I MRD BVW CG 2 CR Heptageniidae 4 34 A MRD BVW SC 3 CG Plecoptera (stoneflies) Perlidae 1 09 A MRD BVW PR 1 CR Trichoptera (caddisflies) Hemiptera (true bugs) (Gerridae) (1) - A MRD BVW ( ) Corixidae 1 09 A MRD BVW PR 5 CR Megaloptera (dobsonflies etc) Coleoptera (beetles) Dryopidae 1 09 A MRD BVW SC 5 CG Diptera (true flies) Tipulidae 2 17 I BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
mm
nn
Other Total Individuals 116
(117) 999
Total Taxa 10 (11)
Notes Subsample taken 6 November 2008 Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009 Gerridae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
- Disclaimer ii
- Title Page iii
- Acknowledgements v
- Table of Contents vii
- SECTION 2 RESULTS DATA TABLES 7
- OVERVIEW AND FINDINGS
- Introduction
- RESULTS DATA TABLES
- REFERENCES
- FIELD DATA SHEETS
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- SubstrateType
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- WATER QUALITY ANALYSIS
- THROUGH THE VIRGINIA
- STREAM CONDITION INDEX (VSCI)
- LABORATORY BENCH SHEETS
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
d
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence x FieldPasture Industrial Obvious sources Agricultural Other ____________ Stream scour steep slope erosion x Residential Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left Field and young thin deciduous forest 100 Bank=Grass cobble and shrub Right 90 maturing deciduous forest 45 shale slope Bank=Bedrock soil and herbs Indicate the dominant vegetation type(s) and record the dominant species
x Trees Shrubs x (Grasses) Forbs Dominant species Oak species and mix deciduous
INSTREAM FEATURES
Measured reach length ___100__m Canopy Cover Estimated stream width _35__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area __350_m2
Estimated stream depth _008_m Proportion of Reach Represented by Stream High water mark _15_m Morphology Types Surface velocity (at thalweg) _05__msec x Riffle__75___ x Run__20___ x Pool__5___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___00__ m2 Density of LWD _-_ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __15_____ x Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present Algae
WATER QUALITY Temperature __19___degC Water Odors x NormalNone Sewage Specific Conductance - Petroleum Chemical Fishy Other___________________ Dissolved Oxygen - Water Surface Oils Slick Sheen Globs Flecks pH __-_ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used _____NA________ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic x None Relict shells Other______None_____ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 8 Detritus sticks wood coarse plant materials
0 Boulder gt256 mm (10rsquorsquo) 0
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 51 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 40 Sand 006-2 mm (gritty) 1 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University
SITE CHARACTERIZATION FIELD DATA SHEET FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Package Plant STATION HLR-2 STREAM CLASS LAT N 39 129580 (upstream) LONG W 078 04900 (upstream) LAT N 39 129581 (downstream) LONG W 078 04822 (downstream) FORM COMPILED BY JW Pangle RIVER BASIN Potomac River INVESTIGATORS JPangle JTovar MDahl B Millholland SRobertson W Bousquet
DATE__19 May 2008_ TIME__1240__ PM
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature _____degC showers (intermittent) Comments __Sunny Day_____________
_5__ cloud cover _0__ ______________________________
x clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x (Significant springs andor seeps) x (Valley) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS
e
f
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence FieldPasture Industrial Obvious sources Agricultural Other ____________ __Upstream Construction and Stream bank Scour Residential __________________________________ Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left Deciduous forest with thick herb and shrub layers bank= vegetation and rocks Right Deciduous forest with thick herb and shrub layers bank=vegetation soil and rocks Indicate the dominant vegetation type(s) and record the dominant species
x Trees x Shrubs Grasses Forbs Dominant species Black Walnut White Ash Hackberry and American Sycamore
INSTREAM FEATURES
Measured reach length 100 m Canopy Cover Estimated stream width _35__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area __350_m2
Estimated stream depth __15__m Proportion of Reach Represented by Stream High water mark __22___m Morphology Types Surface velocity (at thalweg) _035__msec x Riffle__30___ x Run__30___ x Pool__40___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___10____ m2 Density of LWD _______ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __30___ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present__________________________________________________________
WATER QUALITY Temperature __18___degC Water Odors x NormalNone Sewage Specific Conductance __-____ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ____-____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used _____NA____ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other___None_____ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach SubstrateType Characteristic
Composition in Sampling Reach
Bedrock 3 Detritus sticks wood coarse plant materials
2 Boulder gt256 mm (10rsquorsquo) 5
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 70 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 22 Sand 006-2 mm (gritty) 0 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiattrsquos Run LOCATION Rt 664 Bridge STATION HLR-3 STREAM CLASS LAT N 39 12934 (upstream) LONG W 078 05019 (upstream) LAT N 39 12958 (downstream) LONG W 078 04958 (downstream) FORM COMPILED BY Sean Robertson RIVER BASIN Potomac River INVESTIGATORS SRobertson BMillholland WBousquet MDahl JPangle
DATE 28 May 2008 TIME 1127 AM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality 2008
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature __14___degC showers (intermittent) x Trace Comments _______________________
_65 x cloud cover ___ ______________________________
x clearsunny __________________________________
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x (Significant springs andor seeps) x (Valley) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS
g
h
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence Some evidence x FieldPasture Industrial x Obvious sources Agricultural x Other Old Package Plant Downstream x Residential Bridge and Road Power line and Pump station Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left 70 field 30 shrubs and deciduous trees Bank=Soil and vegetation Right Maturing deciduous forest steep slope Bank=Shale Bedrock Indicate the dominant vegetation type(s) and record the dominant species
x Trees x Shrubs x Grasses Forbs Dominant species Sycamore and Red Oaks
INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width __51___m Fully open x Partly open Partly shaded Fully shaded Sampling reach area _510_m2
Estimated stream depth __135___m Proportion of Reach Represented by Stream High water mark __-___m Morphology Types Surface velocity (at thalweg) __20___msec x Riffle_40_ x Run_50_ x Pool 10 Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___0____ m2 Density of LWD ___-____ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation ___50____ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present Brown Algae
WATER QUALITY Temperature __17___degC Water Odors x NormalNone Sewage Specific Conductance ___-___ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ___-_____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used ____NA_______ Clear x Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other____None____ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 15 Detritus sticks wood coarse plant materials
0 Boulder gt256 mm (10rsquorsquo) 15
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 62 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 8 Sand 006-2 mm (gritty) 0 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Unnamed Tributary STATION HLR-4 STREAM CLASS LAT N 39 13035 (upstream) LONG W 078 05129 (upstream) LAT N 39 13000 (downstream) LONG W 078 05086 (downstream) FORM COMPILED BY Marie Dahl RIVER BASIN Potomac River INVESTIGATORS M Dahl HKepple SRobertson AFisher
DATE 31 May 2008 TIME 5 PM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality 2008
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) YES x NO
rain (steady rain) Air Temperature __22___degC showers (intermittent) Comments _______________________
_0__ cloud cover _0__ ______________________________
x clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area __-___ km2 mi2 Montane Significant springs andor seeps x Valley Marsh or swamp Montane amp Valley Mixture of origins Other ___________________
COMMENTS Snowden Bridge development (upstream) and sewer line
i
j
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence x FieldPasture Industrial Obvious sources Agricultural Other ____________ Construction upstream and adjacent x Residential Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left Forested with rocky banks field across silt fence and 2m tree strip Right Forested with some rocky banks and forest through the highway Indicate the dominant vegetation type(s) and record the dominant species
x Trees x Shrubs x Grasses Forbs Dominant species White Ash and Hackberry
INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width ___38__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area __380__m2
Estimated stream depth __12__cm Proportion of Reach Represented by Stream High water mark __2___m Morphology Types Surface velocity (at thalweg) __46___msec x Riffle__25___ x Run__70___ x Pool__5___ Channelized Yes x No Dam Present x Yes No
LARGE WOODY DEBRIS
LWD _______ m2 Density of LWD _______ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation ___NA____ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present___Algae___
WATER QUALITY Temperature __16___degC Water Odors x NormalNone Sewage Specific Conductance __-____ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ___-_____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used ____NA_____ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells x Other___Shale_______ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 0 Detritus sticks wood coarse plant materials
10 Boulder gt256 mm (10rsquorsquo) 5
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 45 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 30 Sand 006-2 mm (gritty) 0 Marl grey shell
fragments 0
Silt 0004-006 mm 20 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Lick Run STATION HLR-5 STREAM CLASS LAT N 39 127517 (upstream) LONG W 078 05685(upstream) LAT N 39 12784 (downstream) LONG W 078 05631 (downstream) FORM COMPILED BY B Millholland RIVER BASIN Potomac River INVESTIGATORS B Millholland JTovar SRobertson MDahl WBousquet JPangle
DATE 29 May 2008 TIME 1040 AM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project
k
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature __15___degC showers (intermittent) Comments _______________________
_5_ X cloud cover _0__ ______________________________
x clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane Significant springs andor seeps x Valley Marsh or swamp Montane amp Valley Mixture of origins Other ___________________
COMMENTS Lick Run appears to be intermittent and may be dry in Fall 2008
l
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence x FieldPasture Industrial Obvious sources Agricultural Other ____________ Adjacent logging stream bank scour Residential Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left 85 young deciduous forest 15 logged Bank=Soil vegetation and cobbles Right Young deciduous forest on 30 slope Bank= Soil vegetation and cobble Indicate the dominant vegetation type(s) and record the dominant species
x Trees Shrubs Grasses Forbs Dominant species Mixed Deciduous INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width __11__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area 110 m2
Estimated stream depth _009__m Proportion of Reach Represented by Stream High water mark __96___m Morphology Types Surface velocity (at thalweg) __01___msec x Riffle__30___ x Run__5___ x Pool__65___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___0____ m2 Density of LWD ___-____ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __70___ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present__________________________________________________________
WATER QUALITY Temperature __14___degC Water Odors x NormalNone Sewage Specific Conductance __-__ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ___-___ Water Surface Oils Slick Sheen Globs Flecks pH __-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used _____NA______ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other_____None_____
SEDIMENT AND SUBSTRATE
Odors Deposits Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other________________ x Other Slightly Salty Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 0 Detritus sticks wood coarse plant materials
5 Boulder gt256 mm (10rsquorsquo) 0
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 75 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 23 Sand 006-2 mm (gritty) 2 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION McCann Farm STATION HLR-6 STREAM CLASS LAT N 39 13076 (upstream) LONG W 078 06539 (upstream) LAT N 39 13063 (downstream) LONG W 078 06473 (downstream) FORM COMPILED BY MDahl RIVER BASIN Potomac River INVESTIGATORS MDahl JPangle SRobertson W Bousqet BMillholland JTovar H Kepple
DATE 30 May 2008 TIME 1050 AM (start)
PROJECT NAME
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) YES x NO
rain (steady rain) Air Temperature __18___degC showers (intermittent) Comments _______________________
_85_x cloud cover _10__ ______________________________
clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x (Significant springs andor seeps) x ( Valley) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS Multi-habitat sampling with D-frame nets and seine
m
n
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial x No evidence Some evidence x FieldPasture Industrial Obvious sources x Agricultural Other ____________ Minor stream bank scour Residential Local Watershed Erosion x None Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left 35 bank grasses scattered trees and some bushes Right High bank with grasses and scattered trees Indicate the dominant vegetation type(s) and record the dominant species
x Trees Shrubs Grasses Forbs Dominant species Black Walnut and Festuca elatior
INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width __2___m Fully open Partly open x Partly shaded Fully shaded Sampling reach area __200_m2
Estimated stream depth __03_m Proportion of Reach Represented by Stream High water mark __1___m Morphology Types Surface velocity (at thalweg) __166_msec x Riffle__0___ x Run__55___ x Pool__45___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___2____ m2 Density of LWD ___-____ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __20_____ x Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present ____Glyceria (grass)sp__________
WATER QUALITY Temperature __16___degC Water Odors x NormalNone Sewage Specific Conductance ___-___ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ____-____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used ______NA___ Clear x Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other__NONE__ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 0 Detritus sticks wood coarse plant materials
5 Boulder gt256 mm (10rsquorsquo) 0
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 27 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 38 Sand 006-2 mm (gritty) 5 Marl grey shell
fragments 0 (lt1)
Silt 0004-006 mm 25 Clay lt 0004 mm (slick) 5 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Appendix B
WATER QUALITY ANALYSIS THROUGH THE VIRGINIA
STREAM CONDITION INDEX (VSCI)
The Virginia Stream Condition Index (VSCI) relies on eight metrics to categorize stream health According to Burton and Gerritsen (2003 p 1-1) this index ldquohellip can be used as a primary indicator of ecosystem health and can identify impairment with respect to the reference (or natural) conditionrdquo These metrics are numeric measures of a biological communityrsquos species composition diversity ecological niches or tolerance to pollution
Table 13 on the next page describes each VSCI metric lists its formula and provides the best standard value (BSV) for that metric Based on a 100-point scale the BSV is the score that reflects optimum conditions for aquatic life ie the best water quality value possible (DEQ 2006b pp 8 33) The table also explains whether the score increases or decreases with environmental stress
As Table 13 indicates each of the eight VSCI metrics is calculated individually Each formula provides a unitless score based on a 100-point scale with 0 as the worst possible score and 100 as the best Any metric that provides a result above 100 is changed to 100 in order for the index to work properly After the eight metrics are calculated they are summed and averaged to provide a final VSCI score on a 100-point scale
Based on statistical analysis of Virginiarsquos streams and their BMI communities the DEQ (2006b p 8) currently uses the following tiers to interpret VSCI scores
VSCI Scores Aquatic Life Use
(ALI) Tiers
lt 42 severely stressed
42-55 moderately stressed
55-63 unknownfair (gray zone)
63-73 good
gt73 excellent
Section 2 of this report presents the results of calculations for the six BMI sampling sites the research team studied in the Hiatt-Lick Run subwatershed in 2008
o
p
1 Total taxa the number of distinct taxa in the sample measures the overall diversity of the macroinvertebrate assemblage The best standard value (BSV) is 189 Score = 100 x (X189) Total taxa decreases with stress 2 EPT taxa the sum of the distinct taxa in the orders Ephemeroptera (mayflies) Plecoptera (stoneflies) and Trichoptera (caddisflies) These orders are known to be sensitive to pollution The best standard value (BSV) is 117 Score = 100 x (X117) EPT taxa decreases with stress 3 Percent Ephemeroptera the percent abundance of individuals in the sample that belong to the order Ephemeroptera (mayflies) The best standard value (BSV) is 477 Score = 100 x (X477) Percent Ephemeroptera decreases with stress 4 Percent Plecoptera + Trichoptera less Hydropsychidae the percent abundance of individuals in the sample that belong to the orders Plecoptera (stoneflies) and Trichoptera (caddisflies) excluding the pollution-tolerant caddisfly family Hydropsychidae The best standard value (BSV) is 562 Score = 100 x (X562) Percent Plecoptera + Trichoptera less Hydropsychidae decreases with stress 5 Percent Scrapers the percent abundance of individuals in the sample whose primary functional mechanism for feeding is grazing on the substrate or periphyton (ie attached algae and associated material) The best standard value (BSV) is 467 Score = 100 x (X467) Percent scrapers decreases with stress 6 Percent Chironomidae the percent abundance of individuals in the sample that are Chironomidae (non-biting midge) larvae and pupae The best standard value (BSV) is 16 Score = 100 x [(100-X)(100-16)] Percent Chironomidae increases with stress 7 Percent Top Two Dominant the percent abundance of the two most frequent taxa present in the sample The best standard value (BSV) is 328 Score = 100 x [(100-X)(100-328)] The Percent Top 2 Dominant increases with stress 8 Hilsenhoff Biotic Index (HBI) an abundance-weighted average pollution tolerance of all the individuals within a sample keyed to the family level Tolerance ratings range from 1 (highly intolerant) to 10 (highly tolerant) The best standard value (BSV) is 29 Score = 100 x [(10-X)(10-29)] The Hilsenhoff Biotic Index (HBI) increases with stress ______________________________________________________________________________ Table 13 Description of Benthic Macroinvertebrate VSCI Metrics Includes responses (increases decreases) of each VSCI metric to degraded water quality conditions Adapted from Burton and Gerritsen (2003 pp 3-15 3-16 6-2) and DEQ (2008)
q
Appendix C
LABORATORY BENCH SHEETS
Shenandoah Universityrsquos Environmental Studies Department developed the Benthic Macroinvertebrate Laboratory Bench Sheet and VSCI Worksheet to record BMI specimen identifications and their VSCI classifications for each sample
This form is based in part on bench sheets developed by the EPA and Virginia DEQ for recording specimen identifications Additional columns on Shenandoah Universityrsquos forms for relative numbers and VSCI classifications make the calculation and verification of VSCI scores easier
Note the column for taxonomistrsquos initials Specimens were identified by students in Shenandoah Universityrsquos research group and then verified or where necessary changed by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality This external review helps assure the validity of the study Originals of these bench sheets with handwritten taxonomistsrsquo initials will be kept on file at Shenandoah Universityrsquos Environmental Studies Department for five years after the completion of this study
r
s
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-1 STATION NAME Mouth LAT N 39deg 12749rsquo (upstream) LONG W 078deg 04209rsquo (upstream) LAT N 39deg 12729rsquo (downstream) LONG W 078deg 04148rsquo (downstream) FORM COMPILED BY Jeremy Tovar W S Bousquet RIVER BASIN Potomac River INVESTIGATORS Marie Dahl Brandon Millholand J W Pangle Sean Robertson Jeremy Tovar Woodward Bousquet Ben Bousquet
Collection date 21 May 2008 Sheet completion date 12 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Hirudinea 3 33 JT BVW PR 8 SP Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Coenagrionidae 2 22 BVW PR 9 CR Ephemeroptera (mayflies) Caenidae 1 11 JT BVW CG 6 SP Ephemerllidae 4 44 JT BVW CG 2 CR Heptageniidae 5 55 JT BVW SC 3 CG Isonychiidae 1 11 BVW CF 3 CG Plecoptera (stoneflies) Perlidae 14 154 JT BVW PR 2 CR Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Corydalidae 1 11 JT BVW PR 5 CR Coleoptera (beetles) Elmidae 24 264 I JT BVW SC 4 CG Dytiscidae 4 44 JT BVW PR 6 GN Psephenidae 3 33 JT BVW SC 4 CG Diptera (true flies) Tipulidae 3 33 JT BVW SH 4 BU Chironomidae 26 286 JT BVW CG 6 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
t
Other Total Individuals 91 1001 Total Taxa 13
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
u
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-1 STATION NAME Mouth LAT N 39deg 12749rsquo (upstream) LONG W 078deg 04209rsquo (upstream) LAT N 39deg 12729rsquo (downstream) LONG W 078deg 04148rsquo (downstream) FORM COMPILED BY Jeremy Tovar W S Bousquet RIVER BASIN Potomac River INVESTIGATORS Brandon Millholand JW Pangle Jeremy Tovar Heather Kepple
Collection date 10 October 2008 Sheet completion date 12 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Oligochaeta 3 35 JT BVW CG 8 BU Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Coenagrionidae 2 23 JT BVW PR 9 CR Ephemeroptera (mayflies) Heptageniidae 12 140 JT BVW SC 3 CG Isonychiidae 2 23 BVW CF 3 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Hydropsychidae 16 186 JT BVW CF 6 CG Philopotamidae 31 360 JT BVW CF 2 CG Hemiptera (true bugs) (Veliidae) (5) - JT BVW ( ) Megaloptera (dobsonflies etc) Corydalidae 10 116 JT BVW PR 5 CR Coleoptera (beetles) Dytiscidae 1 12 JT BVW PR 6 GN Psephenidae 7 81 JT BVW SC 4 CG Diptera (true flies) Tipulidae 2 23 BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
v
Other Total Individuals 86
(91) 999
Total Taxa 10 (11)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009 Veliidae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
w
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-2 STATION NAME Package Plant LAT N 39deg 12958rsquo (upstream) LONG W 078deg 04900 (upstream) LAT N 39deg 12958rsquo (downstream) LONG W 078deg 04822 (downstream) FORM COMPILED BY JW Pangle W Bousquet RIVER BASIN Potomac River INVESTIGATORS JW Pangle S Robertson J Tovar M Dahl B Millholland W Bousquet
Collection date 29 May 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Plecoptera (stoneflies) Perlidae 11 136 I JP BVW PR 1 CR Trichoptera (caddisflies) Hydropsychidae 10 123 I JP BVW CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 14 173 I JP BVW SC 4 CG Diptera (true flies) Chironomidae 32 395 I JP BVW CG 6 BU Simuliidae 14 173 I JP BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
x
Other Total Individuals 81 1000 Total Taxa 5
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-2 STATION NAME Package Plant LAT N 39deg 12958rsquo (upstream) LONG W 078deg 04900 (upstream) LAT N 39deg 12958rsquo (downstream) LONG W 078deg 04822 (downstream) FORM COMPILED BY JW Pangle W Bousquet RIVER BASIN Potomac River INVESTIGATORS JW Pangle Brandon Millholland Jeremy Tovar Heather Kepple
Collection date 10 October 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Heptageniidae 40 392 I WBJP SC 3 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Hydropsychidae 7 69 I JP WB BVW CF 6 CG Philopotamidae 28 275 I JP WB BVW CF 2 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Corydalidae 6 59 I JP WB BVW PR 5 CR Coleoptera (beetles) Psephenidae 7 69 I JP WB BVW SC 4 CG Elmidae 8 78 I JP WB BVW SC 4 CG Diptera (true flies) Tipulidae 6 59 I JP WB BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
y
z
Other Total Individuals 102 1001 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-3 STATION NAME Rt 664 Bridge LAT N 39deg 12934rsquo (upstream) LONG W 078deg 05019rsquo (upstream) LAT N 39deg 12951rsquo (downstream) LONG W 078deg 04958rsquo (downstream) FORM COMPILED BY S Robertson W Bousquet RIVER BASIN Potomac River INVESTIGATORS SRobertson BMillholland WBousquet MDahl JPangle
Collection date 28 May 2008 Sheet completion date 11 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Leptophlebiidae 1 15 I SGR BVW CG 2 CR Baetidae 1 15 I BVW CG 5 CG Plecoptera (stoneflies) Perlidae 3 46 I SGR BVW PR 1 CR Nemouridae 1 15 I BVW SH 3 CR Trichoptera (caddisflies) Hydropsychidae 5 77 I SGR BVW CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 27 415 I SGR BVW SC 4 CG Psephenidae 2 31 A SGR BVW SC 4 CG Diptera (true flies) Simuliidae 5 77 I SGR BVW CF 6 CG Chironomidae 20 308 I SGR BVW CG 6 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
aa
bb
Other Total Individuals 65 999 Total Taxa 9
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-3 STATION NAME Rt 664 Bridge LAT N 39deg 12934rsquo (upstream) LONG W 078deg 05019rsquo (upstream) LAT N 39deg 12951rsquo (downstream) LONG W 078deg 04958rsquo (downstream) FORM COMPILED BY S Robertson RIVER BASIN Potomac River INVESTIGATORS S Robertson M Dahl A Fisher H Kepple
Collection date 10 Oct 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Heptageniidae 2 20 I SGR SC 3 CG Isonychiidae 2 20 I BVW CF 2 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Philopotamidae 36 367 I SGR CF 2 CG Hydropsychidae 47 480 I SGR CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 2 20 A SGR SC 4 CG Psephenidae 8 82 A SGR SC 4 CG Diptera (true flies) Tabanidae 1 10 I SGR PR 7 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
cc
dd
Other Total Individuals 98 999 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-4 STATION NAME Unnamed Tributary LAT (upstream) N 39deg 13035rsquo LONG (upstream) W 078deg 05129rsquo LAT (downstream) N 39deg 13000rsquo LONG (downstream) W 078deg 5086rsquo FORM COMPILED BY A Fisher M Dahl rev W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson A Fisher H Kepple
Collection date 31 May 2008 Sheet completion date rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Flatworms (Turbellaria) Worms (Oligochaeta) Naididae 1 14 - BVW CG 8 BU Leeches (Hirudinea) Snails (Gastropoda) Clams (Bivalvia) Isopods (Isopoda) Asellidae 3 42 A AF BVW CG 8 SP Scuds (Amphipoda) Crayfish Shrimp (Decapoda) Water Mites (Hydracarina) Dragonflies (Odonata Anisoptera) Damselflies (Odonata Zygoptera) Mayflies (Ephemeroptera) Stoneflies (Plecoptera) Perlidae 2 28 I AF BVW PR 1 CR Caddisflies (Trichoptera) True Bugs (Hemiptera) Corixidae 1 14 I AF BVW MP 9 DV Dobsonflies (Megaloptera) Beetles (Coleoptera) Dytiscidae 1 14 I AF BVW PR 6 GN True Flies (Diptera) Chironomidae 61 847 I AF BVW CG 6 BU Simuliidae 3 42 I AF BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
ee
ff
Other Total Individuals 72 1001 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-4 STATION NAME Unnamed Tributary LAT (upstream) N 39deg 13035rsquo LONG (upstream) W 078deg 05129rsquo LAT (downstream) N 39deg 13000rsquo LONG (downstream) W 078deg 5086rsquo FORM COMPILED BY Andrew G M Fisher W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson A Fisher H Kepple
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Flatworms (Turbellaria) Worms (Oligochaeta) Lumbriculidae 2 32 A AF BVW CG 8 BU Leeches (Hirudinea) Snails (Gastropoda) Lymnaeidae 1 16 A BVW CG 9 GN Clams (Bivalvia) Isopods (Isopoda) Scuds (Amphipoda) Crayfish Shrimp (Decapoda) Cambaridae 1 16 A AF BVW GN 5 GN Water Mites (Hydracarina) Dragonflies (Odonata Anisoptera) Damselflies (Odonata Zygoptera) Calopterygidae 4 63 I BVW PR 7 CL Mayflies (Ephemeroptera) Tricorythidae 1 16 I BVW CG 5 SP Stoneflies (Plecoptera) Caddisflies (Trichoptera) Phryganeidae 4 63 I AF BVW SH 4 CR Hydropsychidae 6 95 I AF BVW CF 6 CG I True Bugs (Hemiptera) Corixidae 8 127 I AF BVW MP 9 DV (Veliidae ) (7) - I AF BVW ( ) Dobsonflies (Megaloptera) Sialidae 3 48 I AF BVW PR 7 SP Corydalidae 2 32 I AF BVW PR 5 CR Beetles (Coleoptera) Haliplidae 1 16 A AF BVW MP 8 GN Dysticidae 7 111 I AF BVW PR 6 GN Hydrophilidae 1 16 A AF BVW PR 8 GN Elmidae 3 48 I BVW SC 4 CG True Flies (Diptera) Chironomidae 8 127 I AF BVW CG 6 BU Simuliidae 7 111 I AF BVW CF 6 CG Tipulidae 4 63 I AF BVW SH 4 GU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
gg
hh
Other Total Individuals 63
(70) 1000
Total Taxa 17 (18)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Veliidae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-5 STATION NAME Lick Run LAT N 39deg 12752rsquo (upstream) LONG W 078deg 05685rsquo (upstream) LAT N 39deg 12784rsquo (downstream) LONG W 078deg 05631rsquo (downstream) FORM COMPILED BY Brandon Millholland W Bousquet RIVER BASIN Potomac River INVESTIGATORS J Pangle S Robertson W Bousquet JTovar M Dahl
Collection date 29 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Gomphidae 1 15 I BVW PR 3 BU Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Leptophlebiidae 11 167 A LBM BVW CG 2 CR Plecoptera (stoneflies) Perlidae 10 152 A LBM BVW PR 1 CR Perlodiae 1 15 A LBM BVW PR 2 CR Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 5 76 A LBM BVW SC 4 CG Elmidae 2 30 I LBM BVW SC 4 CG Diptera (true flies) (Culicidae) (3) - P LMB BVW ( ) Chironomidae 23 348 I LBM BVW CG 6 BU Simuliidae 13 197 I LBM BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
ii
jj
Other Total Individuals 66
(69) 1000
Total Taxa 7 (8)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Culicidae (Diptera) pupae not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl J Pangle S Robertson W Bousquet B Millholland J Tovar H Kepple
Collection date 30 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 1 12 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Asellidae 114 965 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Plecoptera (stoneflies) Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 2 23 A MRD BVW SC 4 CG Diptera (true flies)
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
kk
ll
Other Total Individuals 117 1000 Total Taxa 3
Notes Subsample taken 29 October 2009 All taxonomic determinations by MRD were confirmed by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson K McCann A Fisher H Kepple A Barrett
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 2 17 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Sphaeriidae 2 17 I MRD BVW CF 8 BU Isopoda (isopods) Asellidae 99 853 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Calopterygidae 2 17 A MRD BVW PR 7 CL Ephemeroptera (mayflies) Leptophlebiidae 2 17 I MRD BVW CG 2 CR Heptageniidae 4 34 A MRD BVW SC 3 CG Plecoptera (stoneflies) Perlidae 1 09 A MRD BVW PR 1 CR Trichoptera (caddisflies) Hemiptera (true bugs) (Gerridae) (1) - A MRD BVW ( ) Corixidae 1 09 A MRD BVW PR 5 CR Megaloptera (dobsonflies etc) Coleoptera (beetles) Dryopidae 1 09 A MRD BVW SC 5 CG Diptera (true flies) Tipulidae 2 17 I BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
mm
nn
Other Total Individuals 116
(117) 999
Total Taxa 10 (11)
Notes Subsample taken 6 November 2008 Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009 Gerridae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
- Disclaimer ii
- Title Page iii
- Acknowledgements v
- Table of Contents vii
- SECTION 2 RESULTS DATA TABLES 7
- OVERVIEW AND FINDINGS
- Introduction
- RESULTS DATA TABLES
- REFERENCES
- FIELD DATA SHEETS
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- SubstrateType
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- WATER QUALITY ANALYSIS
- THROUGH THE VIRGINIA
- STREAM CONDITION INDEX (VSCI)
- LABORATORY BENCH SHEETS
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
Environmental Studies Department Shenandoah University
SITE CHARACTERIZATION FIELD DATA SHEET FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Package Plant STATION HLR-2 STREAM CLASS LAT N 39 129580 (upstream) LONG W 078 04900 (upstream) LAT N 39 129581 (downstream) LONG W 078 04822 (downstream) FORM COMPILED BY JW Pangle RIVER BASIN Potomac River INVESTIGATORS JPangle JTovar MDahl B Millholland SRobertson W Bousquet
DATE__19 May 2008_ TIME__1240__ PM
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature _____degC showers (intermittent) Comments __Sunny Day_____________
_5__ cloud cover _0__ ______________________________
x clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x (Significant springs andor seeps) x (Valley) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS
e
f
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence FieldPasture Industrial Obvious sources Agricultural Other ____________ __Upstream Construction and Stream bank Scour Residential __________________________________ Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left Deciduous forest with thick herb and shrub layers bank= vegetation and rocks Right Deciduous forest with thick herb and shrub layers bank=vegetation soil and rocks Indicate the dominant vegetation type(s) and record the dominant species
x Trees x Shrubs Grasses Forbs Dominant species Black Walnut White Ash Hackberry and American Sycamore
INSTREAM FEATURES
Measured reach length 100 m Canopy Cover Estimated stream width _35__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area __350_m2
Estimated stream depth __15__m Proportion of Reach Represented by Stream High water mark __22___m Morphology Types Surface velocity (at thalweg) _035__msec x Riffle__30___ x Run__30___ x Pool__40___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___10____ m2 Density of LWD _______ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __30___ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present__________________________________________________________
WATER QUALITY Temperature __18___degC Water Odors x NormalNone Sewage Specific Conductance __-____ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ____-____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used _____NA____ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other___None_____ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach SubstrateType Characteristic
Composition in Sampling Reach
Bedrock 3 Detritus sticks wood coarse plant materials
2 Boulder gt256 mm (10rsquorsquo) 5
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 70 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 22 Sand 006-2 mm (gritty) 0 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiattrsquos Run LOCATION Rt 664 Bridge STATION HLR-3 STREAM CLASS LAT N 39 12934 (upstream) LONG W 078 05019 (upstream) LAT N 39 12958 (downstream) LONG W 078 04958 (downstream) FORM COMPILED BY Sean Robertson RIVER BASIN Potomac River INVESTIGATORS SRobertson BMillholland WBousquet MDahl JPangle
DATE 28 May 2008 TIME 1127 AM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality 2008
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature __14___degC showers (intermittent) x Trace Comments _______________________
_65 x cloud cover ___ ______________________________
x clearsunny __________________________________
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x (Significant springs andor seeps) x (Valley) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS
g
h
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence Some evidence x FieldPasture Industrial x Obvious sources Agricultural x Other Old Package Plant Downstream x Residential Bridge and Road Power line and Pump station Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left 70 field 30 shrubs and deciduous trees Bank=Soil and vegetation Right Maturing deciduous forest steep slope Bank=Shale Bedrock Indicate the dominant vegetation type(s) and record the dominant species
x Trees x Shrubs x Grasses Forbs Dominant species Sycamore and Red Oaks
INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width __51___m Fully open x Partly open Partly shaded Fully shaded Sampling reach area _510_m2
Estimated stream depth __135___m Proportion of Reach Represented by Stream High water mark __-___m Morphology Types Surface velocity (at thalweg) __20___msec x Riffle_40_ x Run_50_ x Pool 10 Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___0____ m2 Density of LWD ___-____ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation ___50____ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present Brown Algae
WATER QUALITY Temperature __17___degC Water Odors x NormalNone Sewage Specific Conductance ___-___ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ___-_____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used ____NA_______ Clear x Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other____None____ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 15 Detritus sticks wood coarse plant materials
0 Boulder gt256 mm (10rsquorsquo) 15
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 62 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 8 Sand 006-2 mm (gritty) 0 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Unnamed Tributary STATION HLR-4 STREAM CLASS LAT N 39 13035 (upstream) LONG W 078 05129 (upstream) LAT N 39 13000 (downstream) LONG W 078 05086 (downstream) FORM COMPILED BY Marie Dahl RIVER BASIN Potomac River INVESTIGATORS M Dahl HKepple SRobertson AFisher
DATE 31 May 2008 TIME 5 PM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality 2008
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) YES x NO
rain (steady rain) Air Temperature __22___degC showers (intermittent) Comments _______________________
_0__ cloud cover _0__ ______________________________
x clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area __-___ km2 mi2 Montane Significant springs andor seeps x Valley Marsh or swamp Montane amp Valley Mixture of origins Other ___________________
COMMENTS Snowden Bridge development (upstream) and sewer line
i
j
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence x FieldPasture Industrial Obvious sources Agricultural Other ____________ Construction upstream and adjacent x Residential Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left Forested with rocky banks field across silt fence and 2m tree strip Right Forested with some rocky banks and forest through the highway Indicate the dominant vegetation type(s) and record the dominant species
x Trees x Shrubs x Grasses Forbs Dominant species White Ash and Hackberry
INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width ___38__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area __380__m2
Estimated stream depth __12__cm Proportion of Reach Represented by Stream High water mark __2___m Morphology Types Surface velocity (at thalweg) __46___msec x Riffle__25___ x Run__70___ x Pool__5___ Channelized Yes x No Dam Present x Yes No
LARGE WOODY DEBRIS
LWD _______ m2 Density of LWD _______ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation ___NA____ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present___Algae___
WATER QUALITY Temperature __16___degC Water Odors x NormalNone Sewage Specific Conductance __-____ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ___-_____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used ____NA_____ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells x Other___Shale_______ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 0 Detritus sticks wood coarse plant materials
10 Boulder gt256 mm (10rsquorsquo) 5
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 45 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 30 Sand 006-2 mm (gritty) 0 Marl grey shell
fragments 0
Silt 0004-006 mm 20 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Lick Run STATION HLR-5 STREAM CLASS LAT N 39 127517 (upstream) LONG W 078 05685(upstream) LAT N 39 12784 (downstream) LONG W 078 05631 (downstream) FORM COMPILED BY B Millholland RIVER BASIN Potomac River INVESTIGATORS B Millholland JTovar SRobertson MDahl WBousquet JPangle
DATE 29 May 2008 TIME 1040 AM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project
k
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature __15___degC showers (intermittent) Comments _______________________
_5_ X cloud cover _0__ ______________________________
x clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane Significant springs andor seeps x Valley Marsh or swamp Montane amp Valley Mixture of origins Other ___________________
COMMENTS Lick Run appears to be intermittent and may be dry in Fall 2008
l
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence x FieldPasture Industrial Obvious sources Agricultural Other ____________ Adjacent logging stream bank scour Residential Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left 85 young deciduous forest 15 logged Bank=Soil vegetation and cobbles Right Young deciduous forest on 30 slope Bank= Soil vegetation and cobble Indicate the dominant vegetation type(s) and record the dominant species
x Trees Shrubs Grasses Forbs Dominant species Mixed Deciduous INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width __11__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area 110 m2
Estimated stream depth _009__m Proportion of Reach Represented by Stream High water mark __96___m Morphology Types Surface velocity (at thalweg) __01___msec x Riffle__30___ x Run__5___ x Pool__65___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___0____ m2 Density of LWD ___-____ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __70___ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present__________________________________________________________
WATER QUALITY Temperature __14___degC Water Odors x NormalNone Sewage Specific Conductance __-__ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ___-___ Water Surface Oils Slick Sheen Globs Flecks pH __-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used _____NA______ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other_____None_____
SEDIMENT AND SUBSTRATE
Odors Deposits Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other________________ x Other Slightly Salty Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 0 Detritus sticks wood coarse plant materials
5 Boulder gt256 mm (10rsquorsquo) 0
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 75 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 23 Sand 006-2 mm (gritty) 2 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION McCann Farm STATION HLR-6 STREAM CLASS LAT N 39 13076 (upstream) LONG W 078 06539 (upstream) LAT N 39 13063 (downstream) LONG W 078 06473 (downstream) FORM COMPILED BY MDahl RIVER BASIN Potomac River INVESTIGATORS MDahl JPangle SRobertson W Bousqet BMillholland JTovar H Kepple
DATE 30 May 2008 TIME 1050 AM (start)
PROJECT NAME
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) YES x NO
rain (steady rain) Air Temperature __18___degC showers (intermittent) Comments _______________________
_85_x cloud cover _10__ ______________________________
clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x (Significant springs andor seeps) x ( Valley) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS Multi-habitat sampling with D-frame nets and seine
m
n
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial x No evidence Some evidence x FieldPasture Industrial Obvious sources x Agricultural Other ____________ Minor stream bank scour Residential Local Watershed Erosion x None Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left 35 bank grasses scattered trees and some bushes Right High bank with grasses and scattered trees Indicate the dominant vegetation type(s) and record the dominant species
x Trees Shrubs Grasses Forbs Dominant species Black Walnut and Festuca elatior
INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width __2___m Fully open Partly open x Partly shaded Fully shaded Sampling reach area __200_m2
Estimated stream depth __03_m Proportion of Reach Represented by Stream High water mark __1___m Morphology Types Surface velocity (at thalweg) __166_msec x Riffle__0___ x Run__55___ x Pool__45___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___2____ m2 Density of LWD ___-____ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __20_____ x Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present ____Glyceria (grass)sp__________
WATER QUALITY Temperature __16___degC Water Odors x NormalNone Sewage Specific Conductance ___-___ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ____-____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used ______NA___ Clear x Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other__NONE__ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 0 Detritus sticks wood coarse plant materials
5 Boulder gt256 mm (10rsquorsquo) 0
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 27 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 38 Sand 006-2 mm (gritty) 5 Marl grey shell
fragments 0 (lt1)
Silt 0004-006 mm 25 Clay lt 0004 mm (slick) 5 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Appendix B
WATER QUALITY ANALYSIS THROUGH THE VIRGINIA
STREAM CONDITION INDEX (VSCI)
The Virginia Stream Condition Index (VSCI) relies on eight metrics to categorize stream health According to Burton and Gerritsen (2003 p 1-1) this index ldquohellip can be used as a primary indicator of ecosystem health and can identify impairment with respect to the reference (or natural) conditionrdquo These metrics are numeric measures of a biological communityrsquos species composition diversity ecological niches or tolerance to pollution
Table 13 on the next page describes each VSCI metric lists its formula and provides the best standard value (BSV) for that metric Based on a 100-point scale the BSV is the score that reflects optimum conditions for aquatic life ie the best water quality value possible (DEQ 2006b pp 8 33) The table also explains whether the score increases or decreases with environmental stress
As Table 13 indicates each of the eight VSCI metrics is calculated individually Each formula provides a unitless score based on a 100-point scale with 0 as the worst possible score and 100 as the best Any metric that provides a result above 100 is changed to 100 in order for the index to work properly After the eight metrics are calculated they are summed and averaged to provide a final VSCI score on a 100-point scale
Based on statistical analysis of Virginiarsquos streams and their BMI communities the DEQ (2006b p 8) currently uses the following tiers to interpret VSCI scores
VSCI Scores Aquatic Life Use
(ALI) Tiers
lt 42 severely stressed
42-55 moderately stressed
55-63 unknownfair (gray zone)
63-73 good
gt73 excellent
Section 2 of this report presents the results of calculations for the six BMI sampling sites the research team studied in the Hiatt-Lick Run subwatershed in 2008
o
p
1 Total taxa the number of distinct taxa in the sample measures the overall diversity of the macroinvertebrate assemblage The best standard value (BSV) is 189 Score = 100 x (X189) Total taxa decreases with stress 2 EPT taxa the sum of the distinct taxa in the orders Ephemeroptera (mayflies) Plecoptera (stoneflies) and Trichoptera (caddisflies) These orders are known to be sensitive to pollution The best standard value (BSV) is 117 Score = 100 x (X117) EPT taxa decreases with stress 3 Percent Ephemeroptera the percent abundance of individuals in the sample that belong to the order Ephemeroptera (mayflies) The best standard value (BSV) is 477 Score = 100 x (X477) Percent Ephemeroptera decreases with stress 4 Percent Plecoptera + Trichoptera less Hydropsychidae the percent abundance of individuals in the sample that belong to the orders Plecoptera (stoneflies) and Trichoptera (caddisflies) excluding the pollution-tolerant caddisfly family Hydropsychidae The best standard value (BSV) is 562 Score = 100 x (X562) Percent Plecoptera + Trichoptera less Hydropsychidae decreases with stress 5 Percent Scrapers the percent abundance of individuals in the sample whose primary functional mechanism for feeding is grazing on the substrate or periphyton (ie attached algae and associated material) The best standard value (BSV) is 467 Score = 100 x (X467) Percent scrapers decreases with stress 6 Percent Chironomidae the percent abundance of individuals in the sample that are Chironomidae (non-biting midge) larvae and pupae The best standard value (BSV) is 16 Score = 100 x [(100-X)(100-16)] Percent Chironomidae increases with stress 7 Percent Top Two Dominant the percent abundance of the two most frequent taxa present in the sample The best standard value (BSV) is 328 Score = 100 x [(100-X)(100-328)] The Percent Top 2 Dominant increases with stress 8 Hilsenhoff Biotic Index (HBI) an abundance-weighted average pollution tolerance of all the individuals within a sample keyed to the family level Tolerance ratings range from 1 (highly intolerant) to 10 (highly tolerant) The best standard value (BSV) is 29 Score = 100 x [(10-X)(10-29)] The Hilsenhoff Biotic Index (HBI) increases with stress ______________________________________________________________________________ Table 13 Description of Benthic Macroinvertebrate VSCI Metrics Includes responses (increases decreases) of each VSCI metric to degraded water quality conditions Adapted from Burton and Gerritsen (2003 pp 3-15 3-16 6-2) and DEQ (2008)
q
Appendix C
LABORATORY BENCH SHEETS
Shenandoah Universityrsquos Environmental Studies Department developed the Benthic Macroinvertebrate Laboratory Bench Sheet and VSCI Worksheet to record BMI specimen identifications and their VSCI classifications for each sample
This form is based in part on bench sheets developed by the EPA and Virginia DEQ for recording specimen identifications Additional columns on Shenandoah Universityrsquos forms for relative numbers and VSCI classifications make the calculation and verification of VSCI scores easier
Note the column for taxonomistrsquos initials Specimens were identified by students in Shenandoah Universityrsquos research group and then verified or where necessary changed by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality This external review helps assure the validity of the study Originals of these bench sheets with handwritten taxonomistsrsquo initials will be kept on file at Shenandoah Universityrsquos Environmental Studies Department for five years after the completion of this study
r
s
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-1 STATION NAME Mouth LAT N 39deg 12749rsquo (upstream) LONG W 078deg 04209rsquo (upstream) LAT N 39deg 12729rsquo (downstream) LONG W 078deg 04148rsquo (downstream) FORM COMPILED BY Jeremy Tovar W S Bousquet RIVER BASIN Potomac River INVESTIGATORS Marie Dahl Brandon Millholand J W Pangle Sean Robertson Jeremy Tovar Woodward Bousquet Ben Bousquet
Collection date 21 May 2008 Sheet completion date 12 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Hirudinea 3 33 JT BVW PR 8 SP Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Coenagrionidae 2 22 BVW PR 9 CR Ephemeroptera (mayflies) Caenidae 1 11 JT BVW CG 6 SP Ephemerllidae 4 44 JT BVW CG 2 CR Heptageniidae 5 55 JT BVW SC 3 CG Isonychiidae 1 11 BVW CF 3 CG Plecoptera (stoneflies) Perlidae 14 154 JT BVW PR 2 CR Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Corydalidae 1 11 JT BVW PR 5 CR Coleoptera (beetles) Elmidae 24 264 I JT BVW SC 4 CG Dytiscidae 4 44 JT BVW PR 6 GN Psephenidae 3 33 JT BVW SC 4 CG Diptera (true flies) Tipulidae 3 33 JT BVW SH 4 BU Chironomidae 26 286 JT BVW CG 6 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
t
Other Total Individuals 91 1001 Total Taxa 13
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
u
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-1 STATION NAME Mouth LAT N 39deg 12749rsquo (upstream) LONG W 078deg 04209rsquo (upstream) LAT N 39deg 12729rsquo (downstream) LONG W 078deg 04148rsquo (downstream) FORM COMPILED BY Jeremy Tovar W S Bousquet RIVER BASIN Potomac River INVESTIGATORS Brandon Millholand JW Pangle Jeremy Tovar Heather Kepple
Collection date 10 October 2008 Sheet completion date 12 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Oligochaeta 3 35 JT BVW CG 8 BU Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Coenagrionidae 2 23 JT BVW PR 9 CR Ephemeroptera (mayflies) Heptageniidae 12 140 JT BVW SC 3 CG Isonychiidae 2 23 BVW CF 3 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Hydropsychidae 16 186 JT BVW CF 6 CG Philopotamidae 31 360 JT BVW CF 2 CG Hemiptera (true bugs) (Veliidae) (5) - JT BVW ( ) Megaloptera (dobsonflies etc) Corydalidae 10 116 JT BVW PR 5 CR Coleoptera (beetles) Dytiscidae 1 12 JT BVW PR 6 GN Psephenidae 7 81 JT BVW SC 4 CG Diptera (true flies) Tipulidae 2 23 BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
v
Other Total Individuals 86
(91) 999
Total Taxa 10 (11)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009 Veliidae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
w
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-2 STATION NAME Package Plant LAT N 39deg 12958rsquo (upstream) LONG W 078deg 04900 (upstream) LAT N 39deg 12958rsquo (downstream) LONG W 078deg 04822 (downstream) FORM COMPILED BY JW Pangle W Bousquet RIVER BASIN Potomac River INVESTIGATORS JW Pangle S Robertson J Tovar M Dahl B Millholland W Bousquet
Collection date 29 May 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Plecoptera (stoneflies) Perlidae 11 136 I JP BVW PR 1 CR Trichoptera (caddisflies) Hydropsychidae 10 123 I JP BVW CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 14 173 I JP BVW SC 4 CG Diptera (true flies) Chironomidae 32 395 I JP BVW CG 6 BU Simuliidae 14 173 I JP BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
x
Other Total Individuals 81 1000 Total Taxa 5
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-2 STATION NAME Package Plant LAT N 39deg 12958rsquo (upstream) LONG W 078deg 04900 (upstream) LAT N 39deg 12958rsquo (downstream) LONG W 078deg 04822 (downstream) FORM COMPILED BY JW Pangle W Bousquet RIVER BASIN Potomac River INVESTIGATORS JW Pangle Brandon Millholland Jeremy Tovar Heather Kepple
Collection date 10 October 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Heptageniidae 40 392 I WBJP SC 3 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Hydropsychidae 7 69 I JP WB BVW CF 6 CG Philopotamidae 28 275 I JP WB BVW CF 2 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Corydalidae 6 59 I JP WB BVW PR 5 CR Coleoptera (beetles) Psephenidae 7 69 I JP WB BVW SC 4 CG Elmidae 8 78 I JP WB BVW SC 4 CG Diptera (true flies) Tipulidae 6 59 I JP WB BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
y
z
Other Total Individuals 102 1001 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-3 STATION NAME Rt 664 Bridge LAT N 39deg 12934rsquo (upstream) LONG W 078deg 05019rsquo (upstream) LAT N 39deg 12951rsquo (downstream) LONG W 078deg 04958rsquo (downstream) FORM COMPILED BY S Robertson W Bousquet RIVER BASIN Potomac River INVESTIGATORS SRobertson BMillholland WBousquet MDahl JPangle
Collection date 28 May 2008 Sheet completion date 11 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Leptophlebiidae 1 15 I SGR BVW CG 2 CR Baetidae 1 15 I BVW CG 5 CG Plecoptera (stoneflies) Perlidae 3 46 I SGR BVW PR 1 CR Nemouridae 1 15 I BVW SH 3 CR Trichoptera (caddisflies) Hydropsychidae 5 77 I SGR BVW CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 27 415 I SGR BVW SC 4 CG Psephenidae 2 31 A SGR BVW SC 4 CG Diptera (true flies) Simuliidae 5 77 I SGR BVW CF 6 CG Chironomidae 20 308 I SGR BVW CG 6 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
aa
bb
Other Total Individuals 65 999 Total Taxa 9
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-3 STATION NAME Rt 664 Bridge LAT N 39deg 12934rsquo (upstream) LONG W 078deg 05019rsquo (upstream) LAT N 39deg 12951rsquo (downstream) LONG W 078deg 04958rsquo (downstream) FORM COMPILED BY S Robertson RIVER BASIN Potomac River INVESTIGATORS S Robertson M Dahl A Fisher H Kepple
Collection date 10 Oct 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Heptageniidae 2 20 I SGR SC 3 CG Isonychiidae 2 20 I BVW CF 2 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Philopotamidae 36 367 I SGR CF 2 CG Hydropsychidae 47 480 I SGR CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 2 20 A SGR SC 4 CG Psephenidae 8 82 A SGR SC 4 CG Diptera (true flies) Tabanidae 1 10 I SGR PR 7 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
cc
dd
Other Total Individuals 98 999 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-4 STATION NAME Unnamed Tributary LAT (upstream) N 39deg 13035rsquo LONG (upstream) W 078deg 05129rsquo LAT (downstream) N 39deg 13000rsquo LONG (downstream) W 078deg 5086rsquo FORM COMPILED BY A Fisher M Dahl rev W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson A Fisher H Kepple
Collection date 31 May 2008 Sheet completion date rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Flatworms (Turbellaria) Worms (Oligochaeta) Naididae 1 14 - BVW CG 8 BU Leeches (Hirudinea) Snails (Gastropoda) Clams (Bivalvia) Isopods (Isopoda) Asellidae 3 42 A AF BVW CG 8 SP Scuds (Amphipoda) Crayfish Shrimp (Decapoda) Water Mites (Hydracarina) Dragonflies (Odonata Anisoptera) Damselflies (Odonata Zygoptera) Mayflies (Ephemeroptera) Stoneflies (Plecoptera) Perlidae 2 28 I AF BVW PR 1 CR Caddisflies (Trichoptera) True Bugs (Hemiptera) Corixidae 1 14 I AF BVW MP 9 DV Dobsonflies (Megaloptera) Beetles (Coleoptera) Dytiscidae 1 14 I AF BVW PR 6 GN True Flies (Diptera) Chironomidae 61 847 I AF BVW CG 6 BU Simuliidae 3 42 I AF BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
ee
ff
Other Total Individuals 72 1001 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-4 STATION NAME Unnamed Tributary LAT (upstream) N 39deg 13035rsquo LONG (upstream) W 078deg 05129rsquo LAT (downstream) N 39deg 13000rsquo LONG (downstream) W 078deg 5086rsquo FORM COMPILED BY Andrew G M Fisher W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson A Fisher H Kepple
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Flatworms (Turbellaria) Worms (Oligochaeta) Lumbriculidae 2 32 A AF BVW CG 8 BU Leeches (Hirudinea) Snails (Gastropoda) Lymnaeidae 1 16 A BVW CG 9 GN Clams (Bivalvia) Isopods (Isopoda) Scuds (Amphipoda) Crayfish Shrimp (Decapoda) Cambaridae 1 16 A AF BVW GN 5 GN Water Mites (Hydracarina) Dragonflies (Odonata Anisoptera) Damselflies (Odonata Zygoptera) Calopterygidae 4 63 I BVW PR 7 CL Mayflies (Ephemeroptera) Tricorythidae 1 16 I BVW CG 5 SP Stoneflies (Plecoptera) Caddisflies (Trichoptera) Phryganeidae 4 63 I AF BVW SH 4 CR Hydropsychidae 6 95 I AF BVW CF 6 CG I True Bugs (Hemiptera) Corixidae 8 127 I AF BVW MP 9 DV (Veliidae ) (7) - I AF BVW ( ) Dobsonflies (Megaloptera) Sialidae 3 48 I AF BVW PR 7 SP Corydalidae 2 32 I AF BVW PR 5 CR Beetles (Coleoptera) Haliplidae 1 16 A AF BVW MP 8 GN Dysticidae 7 111 I AF BVW PR 6 GN Hydrophilidae 1 16 A AF BVW PR 8 GN Elmidae 3 48 I BVW SC 4 CG True Flies (Diptera) Chironomidae 8 127 I AF BVW CG 6 BU Simuliidae 7 111 I AF BVW CF 6 CG Tipulidae 4 63 I AF BVW SH 4 GU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
gg
hh
Other Total Individuals 63
(70) 1000
Total Taxa 17 (18)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Veliidae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-5 STATION NAME Lick Run LAT N 39deg 12752rsquo (upstream) LONG W 078deg 05685rsquo (upstream) LAT N 39deg 12784rsquo (downstream) LONG W 078deg 05631rsquo (downstream) FORM COMPILED BY Brandon Millholland W Bousquet RIVER BASIN Potomac River INVESTIGATORS J Pangle S Robertson W Bousquet JTovar M Dahl
Collection date 29 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Gomphidae 1 15 I BVW PR 3 BU Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Leptophlebiidae 11 167 A LBM BVW CG 2 CR Plecoptera (stoneflies) Perlidae 10 152 A LBM BVW PR 1 CR Perlodiae 1 15 A LBM BVW PR 2 CR Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 5 76 A LBM BVW SC 4 CG Elmidae 2 30 I LBM BVW SC 4 CG Diptera (true flies) (Culicidae) (3) - P LMB BVW ( ) Chironomidae 23 348 I LBM BVW CG 6 BU Simuliidae 13 197 I LBM BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
ii
jj
Other Total Individuals 66
(69) 1000
Total Taxa 7 (8)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Culicidae (Diptera) pupae not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl J Pangle S Robertson W Bousquet B Millholland J Tovar H Kepple
Collection date 30 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 1 12 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Asellidae 114 965 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Plecoptera (stoneflies) Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 2 23 A MRD BVW SC 4 CG Diptera (true flies)
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
kk
ll
Other Total Individuals 117 1000 Total Taxa 3
Notes Subsample taken 29 October 2009 All taxonomic determinations by MRD were confirmed by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson K McCann A Fisher H Kepple A Barrett
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 2 17 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Sphaeriidae 2 17 I MRD BVW CF 8 BU Isopoda (isopods) Asellidae 99 853 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Calopterygidae 2 17 A MRD BVW PR 7 CL Ephemeroptera (mayflies) Leptophlebiidae 2 17 I MRD BVW CG 2 CR Heptageniidae 4 34 A MRD BVW SC 3 CG Plecoptera (stoneflies) Perlidae 1 09 A MRD BVW PR 1 CR Trichoptera (caddisflies) Hemiptera (true bugs) (Gerridae) (1) - A MRD BVW ( ) Corixidae 1 09 A MRD BVW PR 5 CR Megaloptera (dobsonflies etc) Coleoptera (beetles) Dryopidae 1 09 A MRD BVW SC 5 CG Diptera (true flies) Tipulidae 2 17 I BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
mm
nn
Other Total Individuals 116
(117) 999
Total Taxa 10 (11)
Notes Subsample taken 6 November 2008 Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009 Gerridae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
- Disclaimer ii
- Title Page iii
- Acknowledgements v
- Table of Contents vii
- SECTION 2 RESULTS DATA TABLES 7
- OVERVIEW AND FINDINGS
- Introduction
- RESULTS DATA TABLES
- REFERENCES
- FIELD DATA SHEETS
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- SubstrateType
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- WATER QUALITY ANALYSIS
- THROUGH THE VIRGINIA
- STREAM CONDITION INDEX (VSCI)
- LABORATORY BENCH SHEETS
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
f
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence FieldPasture Industrial Obvious sources Agricultural Other ____________ __Upstream Construction and Stream bank Scour Residential __________________________________ Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left Deciduous forest with thick herb and shrub layers bank= vegetation and rocks Right Deciduous forest with thick herb and shrub layers bank=vegetation soil and rocks Indicate the dominant vegetation type(s) and record the dominant species
x Trees x Shrubs Grasses Forbs Dominant species Black Walnut White Ash Hackberry and American Sycamore
INSTREAM FEATURES
Measured reach length 100 m Canopy Cover Estimated stream width _35__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area __350_m2
Estimated stream depth __15__m Proportion of Reach Represented by Stream High water mark __22___m Morphology Types Surface velocity (at thalweg) _035__msec x Riffle__30___ x Run__30___ x Pool__40___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___10____ m2 Density of LWD _______ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __30___ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present__________________________________________________________
WATER QUALITY Temperature __18___degC Water Odors x NormalNone Sewage Specific Conductance __-____ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ____-____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used _____NA____ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other___None_____ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach SubstrateType Characteristic
Composition in Sampling Reach
Bedrock 3 Detritus sticks wood coarse plant materials
2 Boulder gt256 mm (10rsquorsquo) 5
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 70 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 22 Sand 006-2 mm (gritty) 0 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiattrsquos Run LOCATION Rt 664 Bridge STATION HLR-3 STREAM CLASS LAT N 39 12934 (upstream) LONG W 078 05019 (upstream) LAT N 39 12958 (downstream) LONG W 078 04958 (downstream) FORM COMPILED BY Sean Robertson RIVER BASIN Potomac River INVESTIGATORS SRobertson BMillholland WBousquet MDahl JPangle
DATE 28 May 2008 TIME 1127 AM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality 2008
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature __14___degC showers (intermittent) x Trace Comments _______________________
_65 x cloud cover ___ ______________________________
x clearsunny __________________________________
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x (Significant springs andor seeps) x (Valley) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS
g
h
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence Some evidence x FieldPasture Industrial x Obvious sources Agricultural x Other Old Package Plant Downstream x Residential Bridge and Road Power line and Pump station Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left 70 field 30 shrubs and deciduous trees Bank=Soil and vegetation Right Maturing deciduous forest steep slope Bank=Shale Bedrock Indicate the dominant vegetation type(s) and record the dominant species
x Trees x Shrubs x Grasses Forbs Dominant species Sycamore and Red Oaks
INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width __51___m Fully open x Partly open Partly shaded Fully shaded Sampling reach area _510_m2
Estimated stream depth __135___m Proportion of Reach Represented by Stream High water mark __-___m Morphology Types Surface velocity (at thalweg) __20___msec x Riffle_40_ x Run_50_ x Pool 10 Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___0____ m2 Density of LWD ___-____ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation ___50____ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present Brown Algae
WATER QUALITY Temperature __17___degC Water Odors x NormalNone Sewage Specific Conductance ___-___ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ___-_____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used ____NA_______ Clear x Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other____None____ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 15 Detritus sticks wood coarse plant materials
0 Boulder gt256 mm (10rsquorsquo) 15
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 62 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 8 Sand 006-2 mm (gritty) 0 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Unnamed Tributary STATION HLR-4 STREAM CLASS LAT N 39 13035 (upstream) LONG W 078 05129 (upstream) LAT N 39 13000 (downstream) LONG W 078 05086 (downstream) FORM COMPILED BY Marie Dahl RIVER BASIN Potomac River INVESTIGATORS M Dahl HKepple SRobertson AFisher
DATE 31 May 2008 TIME 5 PM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality 2008
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) YES x NO
rain (steady rain) Air Temperature __22___degC showers (intermittent) Comments _______________________
_0__ cloud cover _0__ ______________________________
x clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area __-___ km2 mi2 Montane Significant springs andor seeps x Valley Marsh or swamp Montane amp Valley Mixture of origins Other ___________________
COMMENTS Snowden Bridge development (upstream) and sewer line
i
j
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence x FieldPasture Industrial Obvious sources Agricultural Other ____________ Construction upstream and adjacent x Residential Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left Forested with rocky banks field across silt fence and 2m tree strip Right Forested with some rocky banks and forest through the highway Indicate the dominant vegetation type(s) and record the dominant species
x Trees x Shrubs x Grasses Forbs Dominant species White Ash and Hackberry
INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width ___38__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area __380__m2
Estimated stream depth __12__cm Proportion of Reach Represented by Stream High water mark __2___m Morphology Types Surface velocity (at thalweg) __46___msec x Riffle__25___ x Run__70___ x Pool__5___ Channelized Yes x No Dam Present x Yes No
LARGE WOODY DEBRIS
LWD _______ m2 Density of LWD _______ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation ___NA____ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present___Algae___
WATER QUALITY Temperature __16___degC Water Odors x NormalNone Sewage Specific Conductance __-____ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ___-_____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used ____NA_____ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells x Other___Shale_______ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 0 Detritus sticks wood coarse plant materials
10 Boulder gt256 mm (10rsquorsquo) 5
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 45 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 30 Sand 006-2 mm (gritty) 0 Marl grey shell
fragments 0
Silt 0004-006 mm 20 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Lick Run STATION HLR-5 STREAM CLASS LAT N 39 127517 (upstream) LONG W 078 05685(upstream) LAT N 39 12784 (downstream) LONG W 078 05631 (downstream) FORM COMPILED BY B Millholland RIVER BASIN Potomac River INVESTIGATORS B Millholland JTovar SRobertson MDahl WBousquet JPangle
DATE 29 May 2008 TIME 1040 AM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project
k
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature __15___degC showers (intermittent) Comments _______________________
_5_ X cloud cover _0__ ______________________________
x clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane Significant springs andor seeps x Valley Marsh or swamp Montane amp Valley Mixture of origins Other ___________________
COMMENTS Lick Run appears to be intermittent and may be dry in Fall 2008
l
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence x FieldPasture Industrial Obvious sources Agricultural Other ____________ Adjacent logging stream bank scour Residential Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left 85 young deciduous forest 15 logged Bank=Soil vegetation and cobbles Right Young deciduous forest on 30 slope Bank= Soil vegetation and cobble Indicate the dominant vegetation type(s) and record the dominant species
x Trees Shrubs Grasses Forbs Dominant species Mixed Deciduous INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width __11__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area 110 m2
Estimated stream depth _009__m Proportion of Reach Represented by Stream High water mark __96___m Morphology Types Surface velocity (at thalweg) __01___msec x Riffle__30___ x Run__5___ x Pool__65___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___0____ m2 Density of LWD ___-____ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __70___ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present__________________________________________________________
WATER QUALITY Temperature __14___degC Water Odors x NormalNone Sewage Specific Conductance __-__ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ___-___ Water Surface Oils Slick Sheen Globs Flecks pH __-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used _____NA______ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other_____None_____
SEDIMENT AND SUBSTRATE
Odors Deposits Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other________________ x Other Slightly Salty Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 0 Detritus sticks wood coarse plant materials
5 Boulder gt256 mm (10rsquorsquo) 0
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 75 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 23 Sand 006-2 mm (gritty) 2 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION McCann Farm STATION HLR-6 STREAM CLASS LAT N 39 13076 (upstream) LONG W 078 06539 (upstream) LAT N 39 13063 (downstream) LONG W 078 06473 (downstream) FORM COMPILED BY MDahl RIVER BASIN Potomac River INVESTIGATORS MDahl JPangle SRobertson W Bousqet BMillholland JTovar H Kepple
DATE 30 May 2008 TIME 1050 AM (start)
PROJECT NAME
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) YES x NO
rain (steady rain) Air Temperature __18___degC showers (intermittent) Comments _______________________
_85_x cloud cover _10__ ______________________________
clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x (Significant springs andor seeps) x ( Valley) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS Multi-habitat sampling with D-frame nets and seine
m
n
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial x No evidence Some evidence x FieldPasture Industrial Obvious sources x Agricultural Other ____________ Minor stream bank scour Residential Local Watershed Erosion x None Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left 35 bank grasses scattered trees and some bushes Right High bank with grasses and scattered trees Indicate the dominant vegetation type(s) and record the dominant species
x Trees Shrubs Grasses Forbs Dominant species Black Walnut and Festuca elatior
INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width __2___m Fully open Partly open x Partly shaded Fully shaded Sampling reach area __200_m2
Estimated stream depth __03_m Proportion of Reach Represented by Stream High water mark __1___m Morphology Types Surface velocity (at thalweg) __166_msec x Riffle__0___ x Run__55___ x Pool__45___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___2____ m2 Density of LWD ___-____ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __20_____ x Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present ____Glyceria (grass)sp__________
WATER QUALITY Temperature __16___degC Water Odors x NormalNone Sewage Specific Conductance ___-___ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ____-____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used ______NA___ Clear x Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other__NONE__ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 0 Detritus sticks wood coarse plant materials
5 Boulder gt256 mm (10rsquorsquo) 0
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 27 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 38 Sand 006-2 mm (gritty) 5 Marl grey shell
fragments 0 (lt1)
Silt 0004-006 mm 25 Clay lt 0004 mm (slick) 5 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Appendix B
WATER QUALITY ANALYSIS THROUGH THE VIRGINIA
STREAM CONDITION INDEX (VSCI)
The Virginia Stream Condition Index (VSCI) relies on eight metrics to categorize stream health According to Burton and Gerritsen (2003 p 1-1) this index ldquohellip can be used as a primary indicator of ecosystem health and can identify impairment with respect to the reference (or natural) conditionrdquo These metrics are numeric measures of a biological communityrsquos species composition diversity ecological niches or tolerance to pollution
Table 13 on the next page describes each VSCI metric lists its formula and provides the best standard value (BSV) for that metric Based on a 100-point scale the BSV is the score that reflects optimum conditions for aquatic life ie the best water quality value possible (DEQ 2006b pp 8 33) The table also explains whether the score increases or decreases with environmental stress
As Table 13 indicates each of the eight VSCI metrics is calculated individually Each formula provides a unitless score based on a 100-point scale with 0 as the worst possible score and 100 as the best Any metric that provides a result above 100 is changed to 100 in order for the index to work properly After the eight metrics are calculated they are summed and averaged to provide a final VSCI score on a 100-point scale
Based on statistical analysis of Virginiarsquos streams and their BMI communities the DEQ (2006b p 8) currently uses the following tiers to interpret VSCI scores
VSCI Scores Aquatic Life Use
(ALI) Tiers
lt 42 severely stressed
42-55 moderately stressed
55-63 unknownfair (gray zone)
63-73 good
gt73 excellent
Section 2 of this report presents the results of calculations for the six BMI sampling sites the research team studied in the Hiatt-Lick Run subwatershed in 2008
o
p
1 Total taxa the number of distinct taxa in the sample measures the overall diversity of the macroinvertebrate assemblage The best standard value (BSV) is 189 Score = 100 x (X189) Total taxa decreases with stress 2 EPT taxa the sum of the distinct taxa in the orders Ephemeroptera (mayflies) Plecoptera (stoneflies) and Trichoptera (caddisflies) These orders are known to be sensitive to pollution The best standard value (BSV) is 117 Score = 100 x (X117) EPT taxa decreases with stress 3 Percent Ephemeroptera the percent abundance of individuals in the sample that belong to the order Ephemeroptera (mayflies) The best standard value (BSV) is 477 Score = 100 x (X477) Percent Ephemeroptera decreases with stress 4 Percent Plecoptera + Trichoptera less Hydropsychidae the percent abundance of individuals in the sample that belong to the orders Plecoptera (stoneflies) and Trichoptera (caddisflies) excluding the pollution-tolerant caddisfly family Hydropsychidae The best standard value (BSV) is 562 Score = 100 x (X562) Percent Plecoptera + Trichoptera less Hydropsychidae decreases with stress 5 Percent Scrapers the percent abundance of individuals in the sample whose primary functional mechanism for feeding is grazing on the substrate or periphyton (ie attached algae and associated material) The best standard value (BSV) is 467 Score = 100 x (X467) Percent scrapers decreases with stress 6 Percent Chironomidae the percent abundance of individuals in the sample that are Chironomidae (non-biting midge) larvae and pupae The best standard value (BSV) is 16 Score = 100 x [(100-X)(100-16)] Percent Chironomidae increases with stress 7 Percent Top Two Dominant the percent abundance of the two most frequent taxa present in the sample The best standard value (BSV) is 328 Score = 100 x [(100-X)(100-328)] The Percent Top 2 Dominant increases with stress 8 Hilsenhoff Biotic Index (HBI) an abundance-weighted average pollution tolerance of all the individuals within a sample keyed to the family level Tolerance ratings range from 1 (highly intolerant) to 10 (highly tolerant) The best standard value (BSV) is 29 Score = 100 x [(10-X)(10-29)] The Hilsenhoff Biotic Index (HBI) increases with stress ______________________________________________________________________________ Table 13 Description of Benthic Macroinvertebrate VSCI Metrics Includes responses (increases decreases) of each VSCI metric to degraded water quality conditions Adapted from Burton and Gerritsen (2003 pp 3-15 3-16 6-2) and DEQ (2008)
q
Appendix C
LABORATORY BENCH SHEETS
Shenandoah Universityrsquos Environmental Studies Department developed the Benthic Macroinvertebrate Laboratory Bench Sheet and VSCI Worksheet to record BMI specimen identifications and their VSCI classifications for each sample
This form is based in part on bench sheets developed by the EPA and Virginia DEQ for recording specimen identifications Additional columns on Shenandoah Universityrsquos forms for relative numbers and VSCI classifications make the calculation and verification of VSCI scores easier
Note the column for taxonomistrsquos initials Specimens were identified by students in Shenandoah Universityrsquos research group and then verified or where necessary changed by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality This external review helps assure the validity of the study Originals of these bench sheets with handwritten taxonomistsrsquo initials will be kept on file at Shenandoah Universityrsquos Environmental Studies Department for five years after the completion of this study
r
s
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-1 STATION NAME Mouth LAT N 39deg 12749rsquo (upstream) LONG W 078deg 04209rsquo (upstream) LAT N 39deg 12729rsquo (downstream) LONG W 078deg 04148rsquo (downstream) FORM COMPILED BY Jeremy Tovar W S Bousquet RIVER BASIN Potomac River INVESTIGATORS Marie Dahl Brandon Millholand J W Pangle Sean Robertson Jeremy Tovar Woodward Bousquet Ben Bousquet
Collection date 21 May 2008 Sheet completion date 12 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Hirudinea 3 33 JT BVW PR 8 SP Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Coenagrionidae 2 22 BVW PR 9 CR Ephemeroptera (mayflies) Caenidae 1 11 JT BVW CG 6 SP Ephemerllidae 4 44 JT BVW CG 2 CR Heptageniidae 5 55 JT BVW SC 3 CG Isonychiidae 1 11 BVW CF 3 CG Plecoptera (stoneflies) Perlidae 14 154 JT BVW PR 2 CR Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Corydalidae 1 11 JT BVW PR 5 CR Coleoptera (beetles) Elmidae 24 264 I JT BVW SC 4 CG Dytiscidae 4 44 JT BVW PR 6 GN Psephenidae 3 33 JT BVW SC 4 CG Diptera (true flies) Tipulidae 3 33 JT BVW SH 4 BU Chironomidae 26 286 JT BVW CG 6 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
t
Other Total Individuals 91 1001 Total Taxa 13
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
u
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-1 STATION NAME Mouth LAT N 39deg 12749rsquo (upstream) LONG W 078deg 04209rsquo (upstream) LAT N 39deg 12729rsquo (downstream) LONG W 078deg 04148rsquo (downstream) FORM COMPILED BY Jeremy Tovar W S Bousquet RIVER BASIN Potomac River INVESTIGATORS Brandon Millholand JW Pangle Jeremy Tovar Heather Kepple
Collection date 10 October 2008 Sheet completion date 12 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Oligochaeta 3 35 JT BVW CG 8 BU Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Coenagrionidae 2 23 JT BVW PR 9 CR Ephemeroptera (mayflies) Heptageniidae 12 140 JT BVW SC 3 CG Isonychiidae 2 23 BVW CF 3 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Hydropsychidae 16 186 JT BVW CF 6 CG Philopotamidae 31 360 JT BVW CF 2 CG Hemiptera (true bugs) (Veliidae) (5) - JT BVW ( ) Megaloptera (dobsonflies etc) Corydalidae 10 116 JT BVW PR 5 CR Coleoptera (beetles) Dytiscidae 1 12 JT BVW PR 6 GN Psephenidae 7 81 JT BVW SC 4 CG Diptera (true flies) Tipulidae 2 23 BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
v
Other Total Individuals 86
(91) 999
Total Taxa 10 (11)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009 Veliidae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
w
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-2 STATION NAME Package Plant LAT N 39deg 12958rsquo (upstream) LONG W 078deg 04900 (upstream) LAT N 39deg 12958rsquo (downstream) LONG W 078deg 04822 (downstream) FORM COMPILED BY JW Pangle W Bousquet RIVER BASIN Potomac River INVESTIGATORS JW Pangle S Robertson J Tovar M Dahl B Millholland W Bousquet
Collection date 29 May 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Plecoptera (stoneflies) Perlidae 11 136 I JP BVW PR 1 CR Trichoptera (caddisflies) Hydropsychidae 10 123 I JP BVW CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 14 173 I JP BVW SC 4 CG Diptera (true flies) Chironomidae 32 395 I JP BVW CG 6 BU Simuliidae 14 173 I JP BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
x
Other Total Individuals 81 1000 Total Taxa 5
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-2 STATION NAME Package Plant LAT N 39deg 12958rsquo (upstream) LONG W 078deg 04900 (upstream) LAT N 39deg 12958rsquo (downstream) LONG W 078deg 04822 (downstream) FORM COMPILED BY JW Pangle W Bousquet RIVER BASIN Potomac River INVESTIGATORS JW Pangle Brandon Millholland Jeremy Tovar Heather Kepple
Collection date 10 October 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Heptageniidae 40 392 I WBJP SC 3 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Hydropsychidae 7 69 I JP WB BVW CF 6 CG Philopotamidae 28 275 I JP WB BVW CF 2 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Corydalidae 6 59 I JP WB BVW PR 5 CR Coleoptera (beetles) Psephenidae 7 69 I JP WB BVW SC 4 CG Elmidae 8 78 I JP WB BVW SC 4 CG Diptera (true flies) Tipulidae 6 59 I JP WB BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
y
z
Other Total Individuals 102 1001 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-3 STATION NAME Rt 664 Bridge LAT N 39deg 12934rsquo (upstream) LONG W 078deg 05019rsquo (upstream) LAT N 39deg 12951rsquo (downstream) LONG W 078deg 04958rsquo (downstream) FORM COMPILED BY S Robertson W Bousquet RIVER BASIN Potomac River INVESTIGATORS SRobertson BMillholland WBousquet MDahl JPangle
Collection date 28 May 2008 Sheet completion date 11 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Leptophlebiidae 1 15 I SGR BVW CG 2 CR Baetidae 1 15 I BVW CG 5 CG Plecoptera (stoneflies) Perlidae 3 46 I SGR BVW PR 1 CR Nemouridae 1 15 I BVW SH 3 CR Trichoptera (caddisflies) Hydropsychidae 5 77 I SGR BVW CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 27 415 I SGR BVW SC 4 CG Psephenidae 2 31 A SGR BVW SC 4 CG Diptera (true flies) Simuliidae 5 77 I SGR BVW CF 6 CG Chironomidae 20 308 I SGR BVW CG 6 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
aa
bb
Other Total Individuals 65 999 Total Taxa 9
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-3 STATION NAME Rt 664 Bridge LAT N 39deg 12934rsquo (upstream) LONG W 078deg 05019rsquo (upstream) LAT N 39deg 12951rsquo (downstream) LONG W 078deg 04958rsquo (downstream) FORM COMPILED BY S Robertson RIVER BASIN Potomac River INVESTIGATORS S Robertson M Dahl A Fisher H Kepple
Collection date 10 Oct 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Heptageniidae 2 20 I SGR SC 3 CG Isonychiidae 2 20 I BVW CF 2 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Philopotamidae 36 367 I SGR CF 2 CG Hydropsychidae 47 480 I SGR CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 2 20 A SGR SC 4 CG Psephenidae 8 82 A SGR SC 4 CG Diptera (true flies) Tabanidae 1 10 I SGR PR 7 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
cc
dd
Other Total Individuals 98 999 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-4 STATION NAME Unnamed Tributary LAT (upstream) N 39deg 13035rsquo LONG (upstream) W 078deg 05129rsquo LAT (downstream) N 39deg 13000rsquo LONG (downstream) W 078deg 5086rsquo FORM COMPILED BY A Fisher M Dahl rev W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson A Fisher H Kepple
Collection date 31 May 2008 Sheet completion date rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Flatworms (Turbellaria) Worms (Oligochaeta) Naididae 1 14 - BVW CG 8 BU Leeches (Hirudinea) Snails (Gastropoda) Clams (Bivalvia) Isopods (Isopoda) Asellidae 3 42 A AF BVW CG 8 SP Scuds (Amphipoda) Crayfish Shrimp (Decapoda) Water Mites (Hydracarina) Dragonflies (Odonata Anisoptera) Damselflies (Odonata Zygoptera) Mayflies (Ephemeroptera) Stoneflies (Plecoptera) Perlidae 2 28 I AF BVW PR 1 CR Caddisflies (Trichoptera) True Bugs (Hemiptera) Corixidae 1 14 I AF BVW MP 9 DV Dobsonflies (Megaloptera) Beetles (Coleoptera) Dytiscidae 1 14 I AF BVW PR 6 GN True Flies (Diptera) Chironomidae 61 847 I AF BVW CG 6 BU Simuliidae 3 42 I AF BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
ee
ff
Other Total Individuals 72 1001 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-4 STATION NAME Unnamed Tributary LAT (upstream) N 39deg 13035rsquo LONG (upstream) W 078deg 05129rsquo LAT (downstream) N 39deg 13000rsquo LONG (downstream) W 078deg 5086rsquo FORM COMPILED BY Andrew G M Fisher W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson A Fisher H Kepple
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Flatworms (Turbellaria) Worms (Oligochaeta) Lumbriculidae 2 32 A AF BVW CG 8 BU Leeches (Hirudinea) Snails (Gastropoda) Lymnaeidae 1 16 A BVW CG 9 GN Clams (Bivalvia) Isopods (Isopoda) Scuds (Amphipoda) Crayfish Shrimp (Decapoda) Cambaridae 1 16 A AF BVW GN 5 GN Water Mites (Hydracarina) Dragonflies (Odonata Anisoptera) Damselflies (Odonata Zygoptera) Calopterygidae 4 63 I BVW PR 7 CL Mayflies (Ephemeroptera) Tricorythidae 1 16 I BVW CG 5 SP Stoneflies (Plecoptera) Caddisflies (Trichoptera) Phryganeidae 4 63 I AF BVW SH 4 CR Hydropsychidae 6 95 I AF BVW CF 6 CG I True Bugs (Hemiptera) Corixidae 8 127 I AF BVW MP 9 DV (Veliidae ) (7) - I AF BVW ( ) Dobsonflies (Megaloptera) Sialidae 3 48 I AF BVW PR 7 SP Corydalidae 2 32 I AF BVW PR 5 CR Beetles (Coleoptera) Haliplidae 1 16 A AF BVW MP 8 GN Dysticidae 7 111 I AF BVW PR 6 GN Hydrophilidae 1 16 A AF BVW PR 8 GN Elmidae 3 48 I BVW SC 4 CG True Flies (Diptera) Chironomidae 8 127 I AF BVW CG 6 BU Simuliidae 7 111 I AF BVW CF 6 CG Tipulidae 4 63 I AF BVW SH 4 GU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
gg
hh
Other Total Individuals 63
(70) 1000
Total Taxa 17 (18)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Veliidae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-5 STATION NAME Lick Run LAT N 39deg 12752rsquo (upstream) LONG W 078deg 05685rsquo (upstream) LAT N 39deg 12784rsquo (downstream) LONG W 078deg 05631rsquo (downstream) FORM COMPILED BY Brandon Millholland W Bousquet RIVER BASIN Potomac River INVESTIGATORS J Pangle S Robertson W Bousquet JTovar M Dahl
Collection date 29 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Gomphidae 1 15 I BVW PR 3 BU Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Leptophlebiidae 11 167 A LBM BVW CG 2 CR Plecoptera (stoneflies) Perlidae 10 152 A LBM BVW PR 1 CR Perlodiae 1 15 A LBM BVW PR 2 CR Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 5 76 A LBM BVW SC 4 CG Elmidae 2 30 I LBM BVW SC 4 CG Diptera (true flies) (Culicidae) (3) - P LMB BVW ( ) Chironomidae 23 348 I LBM BVW CG 6 BU Simuliidae 13 197 I LBM BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
ii
jj
Other Total Individuals 66
(69) 1000
Total Taxa 7 (8)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Culicidae (Diptera) pupae not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl J Pangle S Robertson W Bousquet B Millholland J Tovar H Kepple
Collection date 30 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 1 12 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Asellidae 114 965 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Plecoptera (stoneflies) Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 2 23 A MRD BVW SC 4 CG Diptera (true flies)
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
kk
ll
Other Total Individuals 117 1000 Total Taxa 3
Notes Subsample taken 29 October 2009 All taxonomic determinations by MRD were confirmed by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson K McCann A Fisher H Kepple A Barrett
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 2 17 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Sphaeriidae 2 17 I MRD BVW CF 8 BU Isopoda (isopods) Asellidae 99 853 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Calopterygidae 2 17 A MRD BVW PR 7 CL Ephemeroptera (mayflies) Leptophlebiidae 2 17 I MRD BVW CG 2 CR Heptageniidae 4 34 A MRD BVW SC 3 CG Plecoptera (stoneflies) Perlidae 1 09 A MRD BVW PR 1 CR Trichoptera (caddisflies) Hemiptera (true bugs) (Gerridae) (1) - A MRD BVW ( ) Corixidae 1 09 A MRD BVW PR 5 CR Megaloptera (dobsonflies etc) Coleoptera (beetles) Dryopidae 1 09 A MRD BVW SC 5 CG Diptera (true flies) Tipulidae 2 17 I BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
mm
nn
Other Total Individuals 116
(117) 999
Total Taxa 10 (11)
Notes Subsample taken 6 November 2008 Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009 Gerridae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
- Disclaimer ii
- Title Page iii
- Acknowledgements v
- Table of Contents vii
- SECTION 2 RESULTS DATA TABLES 7
- OVERVIEW AND FINDINGS
- Introduction
- RESULTS DATA TABLES
- REFERENCES
- FIELD DATA SHEETS
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- SubstrateType
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- WATER QUALITY ANALYSIS
- THROUGH THE VIRGINIA
- STREAM CONDITION INDEX (VSCI)
- LABORATORY BENCH SHEETS
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiattrsquos Run LOCATION Rt 664 Bridge STATION HLR-3 STREAM CLASS LAT N 39 12934 (upstream) LONG W 078 05019 (upstream) LAT N 39 12958 (downstream) LONG W 078 04958 (downstream) FORM COMPILED BY Sean Robertson RIVER BASIN Potomac River INVESTIGATORS SRobertson BMillholland WBousquet MDahl JPangle
DATE 28 May 2008 TIME 1127 AM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality 2008
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature __14___degC showers (intermittent) x Trace Comments _______________________
_65 x cloud cover ___ ______________________________
x clearsunny __________________________________
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x (Significant springs andor seeps) x (Valley) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS
g
h
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence Some evidence x FieldPasture Industrial x Obvious sources Agricultural x Other Old Package Plant Downstream x Residential Bridge and Road Power line and Pump station Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left 70 field 30 shrubs and deciduous trees Bank=Soil and vegetation Right Maturing deciduous forest steep slope Bank=Shale Bedrock Indicate the dominant vegetation type(s) and record the dominant species
x Trees x Shrubs x Grasses Forbs Dominant species Sycamore and Red Oaks
INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width __51___m Fully open x Partly open Partly shaded Fully shaded Sampling reach area _510_m2
Estimated stream depth __135___m Proportion of Reach Represented by Stream High water mark __-___m Morphology Types Surface velocity (at thalweg) __20___msec x Riffle_40_ x Run_50_ x Pool 10 Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___0____ m2 Density of LWD ___-____ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation ___50____ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present Brown Algae
WATER QUALITY Temperature __17___degC Water Odors x NormalNone Sewage Specific Conductance ___-___ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ___-_____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used ____NA_______ Clear x Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other____None____ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 15 Detritus sticks wood coarse plant materials
0 Boulder gt256 mm (10rsquorsquo) 15
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 62 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 8 Sand 006-2 mm (gritty) 0 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Unnamed Tributary STATION HLR-4 STREAM CLASS LAT N 39 13035 (upstream) LONG W 078 05129 (upstream) LAT N 39 13000 (downstream) LONG W 078 05086 (downstream) FORM COMPILED BY Marie Dahl RIVER BASIN Potomac River INVESTIGATORS M Dahl HKepple SRobertson AFisher
DATE 31 May 2008 TIME 5 PM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality 2008
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) YES x NO
rain (steady rain) Air Temperature __22___degC showers (intermittent) Comments _______________________
_0__ cloud cover _0__ ______________________________
x clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area __-___ km2 mi2 Montane Significant springs andor seeps x Valley Marsh or swamp Montane amp Valley Mixture of origins Other ___________________
COMMENTS Snowden Bridge development (upstream) and sewer line
i
j
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence x FieldPasture Industrial Obvious sources Agricultural Other ____________ Construction upstream and adjacent x Residential Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left Forested with rocky banks field across silt fence and 2m tree strip Right Forested with some rocky banks and forest through the highway Indicate the dominant vegetation type(s) and record the dominant species
x Trees x Shrubs x Grasses Forbs Dominant species White Ash and Hackberry
INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width ___38__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area __380__m2
Estimated stream depth __12__cm Proportion of Reach Represented by Stream High water mark __2___m Morphology Types Surface velocity (at thalweg) __46___msec x Riffle__25___ x Run__70___ x Pool__5___ Channelized Yes x No Dam Present x Yes No
LARGE WOODY DEBRIS
LWD _______ m2 Density of LWD _______ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation ___NA____ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present___Algae___
WATER QUALITY Temperature __16___degC Water Odors x NormalNone Sewage Specific Conductance __-____ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ___-_____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used ____NA_____ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells x Other___Shale_______ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 0 Detritus sticks wood coarse plant materials
10 Boulder gt256 mm (10rsquorsquo) 5
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 45 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 30 Sand 006-2 mm (gritty) 0 Marl grey shell
fragments 0
Silt 0004-006 mm 20 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Lick Run STATION HLR-5 STREAM CLASS LAT N 39 127517 (upstream) LONG W 078 05685(upstream) LAT N 39 12784 (downstream) LONG W 078 05631 (downstream) FORM COMPILED BY B Millholland RIVER BASIN Potomac River INVESTIGATORS B Millholland JTovar SRobertson MDahl WBousquet JPangle
DATE 29 May 2008 TIME 1040 AM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project
k
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature __15___degC showers (intermittent) Comments _______________________
_5_ X cloud cover _0__ ______________________________
x clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane Significant springs andor seeps x Valley Marsh or swamp Montane amp Valley Mixture of origins Other ___________________
COMMENTS Lick Run appears to be intermittent and may be dry in Fall 2008
l
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence x FieldPasture Industrial Obvious sources Agricultural Other ____________ Adjacent logging stream bank scour Residential Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left 85 young deciduous forest 15 logged Bank=Soil vegetation and cobbles Right Young deciduous forest on 30 slope Bank= Soil vegetation and cobble Indicate the dominant vegetation type(s) and record the dominant species
x Trees Shrubs Grasses Forbs Dominant species Mixed Deciduous INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width __11__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area 110 m2
Estimated stream depth _009__m Proportion of Reach Represented by Stream High water mark __96___m Morphology Types Surface velocity (at thalweg) __01___msec x Riffle__30___ x Run__5___ x Pool__65___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___0____ m2 Density of LWD ___-____ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __70___ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present__________________________________________________________
WATER QUALITY Temperature __14___degC Water Odors x NormalNone Sewage Specific Conductance __-__ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ___-___ Water Surface Oils Slick Sheen Globs Flecks pH __-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used _____NA______ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other_____None_____
SEDIMENT AND SUBSTRATE
Odors Deposits Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other________________ x Other Slightly Salty Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 0 Detritus sticks wood coarse plant materials
5 Boulder gt256 mm (10rsquorsquo) 0
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 75 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 23 Sand 006-2 mm (gritty) 2 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION McCann Farm STATION HLR-6 STREAM CLASS LAT N 39 13076 (upstream) LONG W 078 06539 (upstream) LAT N 39 13063 (downstream) LONG W 078 06473 (downstream) FORM COMPILED BY MDahl RIVER BASIN Potomac River INVESTIGATORS MDahl JPangle SRobertson W Bousqet BMillholland JTovar H Kepple
DATE 30 May 2008 TIME 1050 AM (start)
PROJECT NAME
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) YES x NO
rain (steady rain) Air Temperature __18___degC showers (intermittent) Comments _______________________
_85_x cloud cover _10__ ______________________________
clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x (Significant springs andor seeps) x ( Valley) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS Multi-habitat sampling with D-frame nets and seine
m
n
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial x No evidence Some evidence x FieldPasture Industrial Obvious sources x Agricultural Other ____________ Minor stream bank scour Residential Local Watershed Erosion x None Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left 35 bank grasses scattered trees and some bushes Right High bank with grasses and scattered trees Indicate the dominant vegetation type(s) and record the dominant species
x Trees Shrubs Grasses Forbs Dominant species Black Walnut and Festuca elatior
INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width __2___m Fully open Partly open x Partly shaded Fully shaded Sampling reach area __200_m2
Estimated stream depth __03_m Proportion of Reach Represented by Stream High water mark __1___m Morphology Types Surface velocity (at thalweg) __166_msec x Riffle__0___ x Run__55___ x Pool__45___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___2____ m2 Density of LWD ___-____ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __20_____ x Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present ____Glyceria (grass)sp__________
WATER QUALITY Temperature __16___degC Water Odors x NormalNone Sewage Specific Conductance ___-___ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ____-____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used ______NA___ Clear x Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other__NONE__ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 0 Detritus sticks wood coarse plant materials
5 Boulder gt256 mm (10rsquorsquo) 0
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 27 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 38 Sand 006-2 mm (gritty) 5 Marl grey shell
fragments 0 (lt1)
Silt 0004-006 mm 25 Clay lt 0004 mm (slick) 5 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Appendix B
WATER QUALITY ANALYSIS THROUGH THE VIRGINIA
STREAM CONDITION INDEX (VSCI)
The Virginia Stream Condition Index (VSCI) relies on eight metrics to categorize stream health According to Burton and Gerritsen (2003 p 1-1) this index ldquohellip can be used as a primary indicator of ecosystem health and can identify impairment with respect to the reference (or natural) conditionrdquo These metrics are numeric measures of a biological communityrsquos species composition diversity ecological niches or tolerance to pollution
Table 13 on the next page describes each VSCI metric lists its formula and provides the best standard value (BSV) for that metric Based on a 100-point scale the BSV is the score that reflects optimum conditions for aquatic life ie the best water quality value possible (DEQ 2006b pp 8 33) The table also explains whether the score increases or decreases with environmental stress
As Table 13 indicates each of the eight VSCI metrics is calculated individually Each formula provides a unitless score based on a 100-point scale with 0 as the worst possible score and 100 as the best Any metric that provides a result above 100 is changed to 100 in order for the index to work properly After the eight metrics are calculated they are summed and averaged to provide a final VSCI score on a 100-point scale
Based on statistical analysis of Virginiarsquos streams and their BMI communities the DEQ (2006b p 8) currently uses the following tiers to interpret VSCI scores
VSCI Scores Aquatic Life Use
(ALI) Tiers
lt 42 severely stressed
42-55 moderately stressed
55-63 unknownfair (gray zone)
63-73 good
gt73 excellent
Section 2 of this report presents the results of calculations for the six BMI sampling sites the research team studied in the Hiatt-Lick Run subwatershed in 2008
o
p
1 Total taxa the number of distinct taxa in the sample measures the overall diversity of the macroinvertebrate assemblage The best standard value (BSV) is 189 Score = 100 x (X189) Total taxa decreases with stress 2 EPT taxa the sum of the distinct taxa in the orders Ephemeroptera (mayflies) Plecoptera (stoneflies) and Trichoptera (caddisflies) These orders are known to be sensitive to pollution The best standard value (BSV) is 117 Score = 100 x (X117) EPT taxa decreases with stress 3 Percent Ephemeroptera the percent abundance of individuals in the sample that belong to the order Ephemeroptera (mayflies) The best standard value (BSV) is 477 Score = 100 x (X477) Percent Ephemeroptera decreases with stress 4 Percent Plecoptera + Trichoptera less Hydropsychidae the percent abundance of individuals in the sample that belong to the orders Plecoptera (stoneflies) and Trichoptera (caddisflies) excluding the pollution-tolerant caddisfly family Hydropsychidae The best standard value (BSV) is 562 Score = 100 x (X562) Percent Plecoptera + Trichoptera less Hydropsychidae decreases with stress 5 Percent Scrapers the percent abundance of individuals in the sample whose primary functional mechanism for feeding is grazing on the substrate or periphyton (ie attached algae and associated material) The best standard value (BSV) is 467 Score = 100 x (X467) Percent scrapers decreases with stress 6 Percent Chironomidae the percent abundance of individuals in the sample that are Chironomidae (non-biting midge) larvae and pupae The best standard value (BSV) is 16 Score = 100 x [(100-X)(100-16)] Percent Chironomidae increases with stress 7 Percent Top Two Dominant the percent abundance of the two most frequent taxa present in the sample The best standard value (BSV) is 328 Score = 100 x [(100-X)(100-328)] The Percent Top 2 Dominant increases with stress 8 Hilsenhoff Biotic Index (HBI) an abundance-weighted average pollution tolerance of all the individuals within a sample keyed to the family level Tolerance ratings range from 1 (highly intolerant) to 10 (highly tolerant) The best standard value (BSV) is 29 Score = 100 x [(10-X)(10-29)] The Hilsenhoff Biotic Index (HBI) increases with stress ______________________________________________________________________________ Table 13 Description of Benthic Macroinvertebrate VSCI Metrics Includes responses (increases decreases) of each VSCI metric to degraded water quality conditions Adapted from Burton and Gerritsen (2003 pp 3-15 3-16 6-2) and DEQ (2008)
q
Appendix C
LABORATORY BENCH SHEETS
Shenandoah Universityrsquos Environmental Studies Department developed the Benthic Macroinvertebrate Laboratory Bench Sheet and VSCI Worksheet to record BMI specimen identifications and their VSCI classifications for each sample
This form is based in part on bench sheets developed by the EPA and Virginia DEQ for recording specimen identifications Additional columns on Shenandoah Universityrsquos forms for relative numbers and VSCI classifications make the calculation and verification of VSCI scores easier
Note the column for taxonomistrsquos initials Specimens were identified by students in Shenandoah Universityrsquos research group and then verified or where necessary changed by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality This external review helps assure the validity of the study Originals of these bench sheets with handwritten taxonomistsrsquo initials will be kept on file at Shenandoah Universityrsquos Environmental Studies Department for five years after the completion of this study
r
s
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-1 STATION NAME Mouth LAT N 39deg 12749rsquo (upstream) LONG W 078deg 04209rsquo (upstream) LAT N 39deg 12729rsquo (downstream) LONG W 078deg 04148rsquo (downstream) FORM COMPILED BY Jeremy Tovar W S Bousquet RIVER BASIN Potomac River INVESTIGATORS Marie Dahl Brandon Millholand J W Pangle Sean Robertson Jeremy Tovar Woodward Bousquet Ben Bousquet
Collection date 21 May 2008 Sheet completion date 12 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Hirudinea 3 33 JT BVW PR 8 SP Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Coenagrionidae 2 22 BVW PR 9 CR Ephemeroptera (mayflies) Caenidae 1 11 JT BVW CG 6 SP Ephemerllidae 4 44 JT BVW CG 2 CR Heptageniidae 5 55 JT BVW SC 3 CG Isonychiidae 1 11 BVW CF 3 CG Plecoptera (stoneflies) Perlidae 14 154 JT BVW PR 2 CR Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Corydalidae 1 11 JT BVW PR 5 CR Coleoptera (beetles) Elmidae 24 264 I JT BVW SC 4 CG Dytiscidae 4 44 JT BVW PR 6 GN Psephenidae 3 33 JT BVW SC 4 CG Diptera (true flies) Tipulidae 3 33 JT BVW SH 4 BU Chironomidae 26 286 JT BVW CG 6 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
t
Other Total Individuals 91 1001 Total Taxa 13
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
u
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-1 STATION NAME Mouth LAT N 39deg 12749rsquo (upstream) LONG W 078deg 04209rsquo (upstream) LAT N 39deg 12729rsquo (downstream) LONG W 078deg 04148rsquo (downstream) FORM COMPILED BY Jeremy Tovar W S Bousquet RIVER BASIN Potomac River INVESTIGATORS Brandon Millholand JW Pangle Jeremy Tovar Heather Kepple
Collection date 10 October 2008 Sheet completion date 12 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Oligochaeta 3 35 JT BVW CG 8 BU Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Coenagrionidae 2 23 JT BVW PR 9 CR Ephemeroptera (mayflies) Heptageniidae 12 140 JT BVW SC 3 CG Isonychiidae 2 23 BVW CF 3 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Hydropsychidae 16 186 JT BVW CF 6 CG Philopotamidae 31 360 JT BVW CF 2 CG Hemiptera (true bugs) (Veliidae) (5) - JT BVW ( ) Megaloptera (dobsonflies etc) Corydalidae 10 116 JT BVW PR 5 CR Coleoptera (beetles) Dytiscidae 1 12 JT BVW PR 6 GN Psephenidae 7 81 JT BVW SC 4 CG Diptera (true flies) Tipulidae 2 23 BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
v
Other Total Individuals 86
(91) 999
Total Taxa 10 (11)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009 Veliidae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
w
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-2 STATION NAME Package Plant LAT N 39deg 12958rsquo (upstream) LONG W 078deg 04900 (upstream) LAT N 39deg 12958rsquo (downstream) LONG W 078deg 04822 (downstream) FORM COMPILED BY JW Pangle W Bousquet RIVER BASIN Potomac River INVESTIGATORS JW Pangle S Robertson J Tovar M Dahl B Millholland W Bousquet
Collection date 29 May 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Plecoptera (stoneflies) Perlidae 11 136 I JP BVW PR 1 CR Trichoptera (caddisflies) Hydropsychidae 10 123 I JP BVW CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 14 173 I JP BVW SC 4 CG Diptera (true flies) Chironomidae 32 395 I JP BVW CG 6 BU Simuliidae 14 173 I JP BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
x
Other Total Individuals 81 1000 Total Taxa 5
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-2 STATION NAME Package Plant LAT N 39deg 12958rsquo (upstream) LONG W 078deg 04900 (upstream) LAT N 39deg 12958rsquo (downstream) LONG W 078deg 04822 (downstream) FORM COMPILED BY JW Pangle W Bousquet RIVER BASIN Potomac River INVESTIGATORS JW Pangle Brandon Millholland Jeremy Tovar Heather Kepple
Collection date 10 October 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Heptageniidae 40 392 I WBJP SC 3 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Hydropsychidae 7 69 I JP WB BVW CF 6 CG Philopotamidae 28 275 I JP WB BVW CF 2 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Corydalidae 6 59 I JP WB BVW PR 5 CR Coleoptera (beetles) Psephenidae 7 69 I JP WB BVW SC 4 CG Elmidae 8 78 I JP WB BVW SC 4 CG Diptera (true flies) Tipulidae 6 59 I JP WB BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
y
z
Other Total Individuals 102 1001 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-3 STATION NAME Rt 664 Bridge LAT N 39deg 12934rsquo (upstream) LONG W 078deg 05019rsquo (upstream) LAT N 39deg 12951rsquo (downstream) LONG W 078deg 04958rsquo (downstream) FORM COMPILED BY S Robertson W Bousquet RIVER BASIN Potomac River INVESTIGATORS SRobertson BMillholland WBousquet MDahl JPangle
Collection date 28 May 2008 Sheet completion date 11 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Leptophlebiidae 1 15 I SGR BVW CG 2 CR Baetidae 1 15 I BVW CG 5 CG Plecoptera (stoneflies) Perlidae 3 46 I SGR BVW PR 1 CR Nemouridae 1 15 I BVW SH 3 CR Trichoptera (caddisflies) Hydropsychidae 5 77 I SGR BVW CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 27 415 I SGR BVW SC 4 CG Psephenidae 2 31 A SGR BVW SC 4 CG Diptera (true flies) Simuliidae 5 77 I SGR BVW CF 6 CG Chironomidae 20 308 I SGR BVW CG 6 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
aa
bb
Other Total Individuals 65 999 Total Taxa 9
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-3 STATION NAME Rt 664 Bridge LAT N 39deg 12934rsquo (upstream) LONG W 078deg 05019rsquo (upstream) LAT N 39deg 12951rsquo (downstream) LONG W 078deg 04958rsquo (downstream) FORM COMPILED BY S Robertson RIVER BASIN Potomac River INVESTIGATORS S Robertson M Dahl A Fisher H Kepple
Collection date 10 Oct 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Heptageniidae 2 20 I SGR SC 3 CG Isonychiidae 2 20 I BVW CF 2 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Philopotamidae 36 367 I SGR CF 2 CG Hydropsychidae 47 480 I SGR CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 2 20 A SGR SC 4 CG Psephenidae 8 82 A SGR SC 4 CG Diptera (true flies) Tabanidae 1 10 I SGR PR 7 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
cc
dd
Other Total Individuals 98 999 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-4 STATION NAME Unnamed Tributary LAT (upstream) N 39deg 13035rsquo LONG (upstream) W 078deg 05129rsquo LAT (downstream) N 39deg 13000rsquo LONG (downstream) W 078deg 5086rsquo FORM COMPILED BY A Fisher M Dahl rev W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson A Fisher H Kepple
Collection date 31 May 2008 Sheet completion date rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Flatworms (Turbellaria) Worms (Oligochaeta) Naididae 1 14 - BVW CG 8 BU Leeches (Hirudinea) Snails (Gastropoda) Clams (Bivalvia) Isopods (Isopoda) Asellidae 3 42 A AF BVW CG 8 SP Scuds (Amphipoda) Crayfish Shrimp (Decapoda) Water Mites (Hydracarina) Dragonflies (Odonata Anisoptera) Damselflies (Odonata Zygoptera) Mayflies (Ephemeroptera) Stoneflies (Plecoptera) Perlidae 2 28 I AF BVW PR 1 CR Caddisflies (Trichoptera) True Bugs (Hemiptera) Corixidae 1 14 I AF BVW MP 9 DV Dobsonflies (Megaloptera) Beetles (Coleoptera) Dytiscidae 1 14 I AF BVW PR 6 GN True Flies (Diptera) Chironomidae 61 847 I AF BVW CG 6 BU Simuliidae 3 42 I AF BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
ee
ff
Other Total Individuals 72 1001 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-4 STATION NAME Unnamed Tributary LAT (upstream) N 39deg 13035rsquo LONG (upstream) W 078deg 05129rsquo LAT (downstream) N 39deg 13000rsquo LONG (downstream) W 078deg 5086rsquo FORM COMPILED BY Andrew G M Fisher W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson A Fisher H Kepple
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Flatworms (Turbellaria) Worms (Oligochaeta) Lumbriculidae 2 32 A AF BVW CG 8 BU Leeches (Hirudinea) Snails (Gastropoda) Lymnaeidae 1 16 A BVW CG 9 GN Clams (Bivalvia) Isopods (Isopoda) Scuds (Amphipoda) Crayfish Shrimp (Decapoda) Cambaridae 1 16 A AF BVW GN 5 GN Water Mites (Hydracarina) Dragonflies (Odonata Anisoptera) Damselflies (Odonata Zygoptera) Calopterygidae 4 63 I BVW PR 7 CL Mayflies (Ephemeroptera) Tricorythidae 1 16 I BVW CG 5 SP Stoneflies (Plecoptera) Caddisflies (Trichoptera) Phryganeidae 4 63 I AF BVW SH 4 CR Hydropsychidae 6 95 I AF BVW CF 6 CG I True Bugs (Hemiptera) Corixidae 8 127 I AF BVW MP 9 DV (Veliidae ) (7) - I AF BVW ( ) Dobsonflies (Megaloptera) Sialidae 3 48 I AF BVW PR 7 SP Corydalidae 2 32 I AF BVW PR 5 CR Beetles (Coleoptera) Haliplidae 1 16 A AF BVW MP 8 GN Dysticidae 7 111 I AF BVW PR 6 GN Hydrophilidae 1 16 A AF BVW PR 8 GN Elmidae 3 48 I BVW SC 4 CG True Flies (Diptera) Chironomidae 8 127 I AF BVW CG 6 BU Simuliidae 7 111 I AF BVW CF 6 CG Tipulidae 4 63 I AF BVW SH 4 GU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
gg
hh
Other Total Individuals 63
(70) 1000
Total Taxa 17 (18)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Veliidae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-5 STATION NAME Lick Run LAT N 39deg 12752rsquo (upstream) LONG W 078deg 05685rsquo (upstream) LAT N 39deg 12784rsquo (downstream) LONG W 078deg 05631rsquo (downstream) FORM COMPILED BY Brandon Millholland W Bousquet RIVER BASIN Potomac River INVESTIGATORS J Pangle S Robertson W Bousquet JTovar M Dahl
Collection date 29 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Gomphidae 1 15 I BVW PR 3 BU Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Leptophlebiidae 11 167 A LBM BVW CG 2 CR Plecoptera (stoneflies) Perlidae 10 152 A LBM BVW PR 1 CR Perlodiae 1 15 A LBM BVW PR 2 CR Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 5 76 A LBM BVW SC 4 CG Elmidae 2 30 I LBM BVW SC 4 CG Diptera (true flies) (Culicidae) (3) - P LMB BVW ( ) Chironomidae 23 348 I LBM BVW CG 6 BU Simuliidae 13 197 I LBM BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
ii
jj
Other Total Individuals 66
(69) 1000
Total Taxa 7 (8)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Culicidae (Diptera) pupae not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl J Pangle S Robertson W Bousquet B Millholland J Tovar H Kepple
Collection date 30 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 1 12 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Asellidae 114 965 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Plecoptera (stoneflies) Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 2 23 A MRD BVW SC 4 CG Diptera (true flies)
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
kk
ll
Other Total Individuals 117 1000 Total Taxa 3
Notes Subsample taken 29 October 2009 All taxonomic determinations by MRD were confirmed by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson K McCann A Fisher H Kepple A Barrett
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 2 17 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Sphaeriidae 2 17 I MRD BVW CF 8 BU Isopoda (isopods) Asellidae 99 853 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Calopterygidae 2 17 A MRD BVW PR 7 CL Ephemeroptera (mayflies) Leptophlebiidae 2 17 I MRD BVW CG 2 CR Heptageniidae 4 34 A MRD BVW SC 3 CG Plecoptera (stoneflies) Perlidae 1 09 A MRD BVW PR 1 CR Trichoptera (caddisflies) Hemiptera (true bugs) (Gerridae) (1) - A MRD BVW ( ) Corixidae 1 09 A MRD BVW PR 5 CR Megaloptera (dobsonflies etc) Coleoptera (beetles) Dryopidae 1 09 A MRD BVW SC 5 CG Diptera (true flies) Tipulidae 2 17 I BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
mm
nn
Other Total Individuals 116
(117) 999
Total Taxa 10 (11)
Notes Subsample taken 6 November 2008 Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009 Gerridae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
- Disclaimer ii
- Title Page iii
- Acknowledgements v
- Table of Contents vii
- SECTION 2 RESULTS DATA TABLES 7
- OVERVIEW AND FINDINGS
- Introduction
- RESULTS DATA TABLES
- REFERENCES
- FIELD DATA SHEETS
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- SubstrateType
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- WATER QUALITY ANALYSIS
- THROUGH THE VIRGINIA
- STREAM CONDITION INDEX (VSCI)
- LABORATORY BENCH SHEETS
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
h
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence Some evidence x FieldPasture Industrial x Obvious sources Agricultural x Other Old Package Plant Downstream x Residential Bridge and Road Power line and Pump station Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left 70 field 30 shrubs and deciduous trees Bank=Soil and vegetation Right Maturing deciduous forest steep slope Bank=Shale Bedrock Indicate the dominant vegetation type(s) and record the dominant species
x Trees x Shrubs x Grasses Forbs Dominant species Sycamore and Red Oaks
INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width __51___m Fully open x Partly open Partly shaded Fully shaded Sampling reach area _510_m2
Estimated stream depth __135___m Proportion of Reach Represented by Stream High water mark __-___m Morphology Types Surface velocity (at thalweg) __20___msec x Riffle_40_ x Run_50_ x Pool 10 Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___0____ m2 Density of LWD ___-____ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation ___50____ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present Brown Algae
WATER QUALITY Temperature __17___degC Water Odors x NormalNone Sewage Specific Conductance ___-___ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ___-_____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used ____NA_______ Clear x Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other____None____ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 15 Detritus sticks wood coarse plant materials
0 Boulder gt256 mm (10rsquorsquo) 15
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 62 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 8 Sand 006-2 mm (gritty) 0 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Unnamed Tributary STATION HLR-4 STREAM CLASS LAT N 39 13035 (upstream) LONG W 078 05129 (upstream) LAT N 39 13000 (downstream) LONG W 078 05086 (downstream) FORM COMPILED BY Marie Dahl RIVER BASIN Potomac River INVESTIGATORS M Dahl HKepple SRobertson AFisher
DATE 31 May 2008 TIME 5 PM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality 2008
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) YES x NO
rain (steady rain) Air Temperature __22___degC showers (intermittent) Comments _______________________
_0__ cloud cover _0__ ______________________________
x clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area __-___ km2 mi2 Montane Significant springs andor seeps x Valley Marsh or swamp Montane amp Valley Mixture of origins Other ___________________
COMMENTS Snowden Bridge development (upstream) and sewer line
i
j
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence x FieldPasture Industrial Obvious sources Agricultural Other ____________ Construction upstream and adjacent x Residential Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left Forested with rocky banks field across silt fence and 2m tree strip Right Forested with some rocky banks and forest through the highway Indicate the dominant vegetation type(s) and record the dominant species
x Trees x Shrubs x Grasses Forbs Dominant species White Ash and Hackberry
INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width ___38__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area __380__m2
Estimated stream depth __12__cm Proportion of Reach Represented by Stream High water mark __2___m Morphology Types Surface velocity (at thalweg) __46___msec x Riffle__25___ x Run__70___ x Pool__5___ Channelized Yes x No Dam Present x Yes No
LARGE WOODY DEBRIS
LWD _______ m2 Density of LWD _______ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation ___NA____ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present___Algae___
WATER QUALITY Temperature __16___degC Water Odors x NormalNone Sewage Specific Conductance __-____ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ___-_____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used ____NA_____ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells x Other___Shale_______ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 0 Detritus sticks wood coarse plant materials
10 Boulder gt256 mm (10rsquorsquo) 5
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 45 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 30 Sand 006-2 mm (gritty) 0 Marl grey shell
fragments 0
Silt 0004-006 mm 20 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Lick Run STATION HLR-5 STREAM CLASS LAT N 39 127517 (upstream) LONG W 078 05685(upstream) LAT N 39 12784 (downstream) LONG W 078 05631 (downstream) FORM COMPILED BY B Millholland RIVER BASIN Potomac River INVESTIGATORS B Millholland JTovar SRobertson MDahl WBousquet JPangle
DATE 29 May 2008 TIME 1040 AM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project
k
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature __15___degC showers (intermittent) Comments _______________________
_5_ X cloud cover _0__ ______________________________
x clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane Significant springs andor seeps x Valley Marsh or swamp Montane amp Valley Mixture of origins Other ___________________
COMMENTS Lick Run appears to be intermittent and may be dry in Fall 2008
l
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence x FieldPasture Industrial Obvious sources Agricultural Other ____________ Adjacent logging stream bank scour Residential Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left 85 young deciduous forest 15 logged Bank=Soil vegetation and cobbles Right Young deciduous forest on 30 slope Bank= Soil vegetation and cobble Indicate the dominant vegetation type(s) and record the dominant species
x Trees Shrubs Grasses Forbs Dominant species Mixed Deciduous INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width __11__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area 110 m2
Estimated stream depth _009__m Proportion of Reach Represented by Stream High water mark __96___m Morphology Types Surface velocity (at thalweg) __01___msec x Riffle__30___ x Run__5___ x Pool__65___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___0____ m2 Density of LWD ___-____ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __70___ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present__________________________________________________________
WATER QUALITY Temperature __14___degC Water Odors x NormalNone Sewage Specific Conductance __-__ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ___-___ Water Surface Oils Slick Sheen Globs Flecks pH __-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used _____NA______ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other_____None_____
SEDIMENT AND SUBSTRATE
Odors Deposits Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other________________ x Other Slightly Salty Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 0 Detritus sticks wood coarse plant materials
5 Boulder gt256 mm (10rsquorsquo) 0
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 75 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 23 Sand 006-2 mm (gritty) 2 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION McCann Farm STATION HLR-6 STREAM CLASS LAT N 39 13076 (upstream) LONG W 078 06539 (upstream) LAT N 39 13063 (downstream) LONG W 078 06473 (downstream) FORM COMPILED BY MDahl RIVER BASIN Potomac River INVESTIGATORS MDahl JPangle SRobertson W Bousqet BMillholland JTovar H Kepple
DATE 30 May 2008 TIME 1050 AM (start)
PROJECT NAME
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) YES x NO
rain (steady rain) Air Temperature __18___degC showers (intermittent) Comments _______________________
_85_x cloud cover _10__ ______________________________
clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x (Significant springs andor seeps) x ( Valley) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS Multi-habitat sampling with D-frame nets and seine
m
n
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial x No evidence Some evidence x FieldPasture Industrial Obvious sources x Agricultural Other ____________ Minor stream bank scour Residential Local Watershed Erosion x None Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left 35 bank grasses scattered trees and some bushes Right High bank with grasses and scattered trees Indicate the dominant vegetation type(s) and record the dominant species
x Trees Shrubs Grasses Forbs Dominant species Black Walnut and Festuca elatior
INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width __2___m Fully open Partly open x Partly shaded Fully shaded Sampling reach area __200_m2
Estimated stream depth __03_m Proportion of Reach Represented by Stream High water mark __1___m Morphology Types Surface velocity (at thalweg) __166_msec x Riffle__0___ x Run__55___ x Pool__45___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___2____ m2 Density of LWD ___-____ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __20_____ x Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present ____Glyceria (grass)sp__________
WATER QUALITY Temperature __16___degC Water Odors x NormalNone Sewage Specific Conductance ___-___ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ____-____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used ______NA___ Clear x Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other__NONE__ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 0 Detritus sticks wood coarse plant materials
5 Boulder gt256 mm (10rsquorsquo) 0
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 27 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 38 Sand 006-2 mm (gritty) 5 Marl grey shell
fragments 0 (lt1)
Silt 0004-006 mm 25 Clay lt 0004 mm (slick) 5 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Appendix B
WATER QUALITY ANALYSIS THROUGH THE VIRGINIA
STREAM CONDITION INDEX (VSCI)
The Virginia Stream Condition Index (VSCI) relies on eight metrics to categorize stream health According to Burton and Gerritsen (2003 p 1-1) this index ldquohellip can be used as a primary indicator of ecosystem health and can identify impairment with respect to the reference (or natural) conditionrdquo These metrics are numeric measures of a biological communityrsquos species composition diversity ecological niches or tolerance to pollution
Table 13 on the next page describes each VSCI metric lists its formula and provides the best standard value (BSV) for that metric Based on a 100-point scale the BSV is the score that reflects optimum conditions for aquatic life ie the best water quality value possible (DEQ 2006b pp 8 33) The table also explains whether the score increases or decreases with environmental stress
As Table 13 indicates each of the eight VSCI metrics is calculated individually Each formula provides a unitless score based on a 100-point scale with 0 as the worst possible score and 100 as the best Any metric that provides a result above 100 is changed to 100 in order for the index to work properly After the eight metrics are calculated they are summed and averaged to provide a final VSCI score on a 100-point scale
Based on statistical analysis of Virginiarsquos streams and their BMI communities the DEQ (2006b p 8) currently uses the following tiers to interpret VSCI scores
VSCI Scores Aquatic Life Use
(ALI) Tiers
lt 42 severely stressed
42-55 moderately stressed
55-63 unknownfair (gray zone)
63-73 good
gt73 excellent
Section 2 of this report presents the results of calculations for the six BMI sampling sites the research team studied in the Hiatt-Lick Run subwatershed in 2008
o
p
1 Total taxa the number of distinct taxa in the sample measures the overall diversity of the macroinvertebrate assemblage The best standard value (BSV) is 189 Score = 100 x (X189) Total taxa decreases with stress 2 EPT taxa the sum of the distinct taxa in the orders Ephemeroptera (mayflies) Plecoptera (stoneflies) and Trichoptera (caddisflies) These orders are known to be sensitive to pollution The best standard value (BSV) is 117 Score = 100 x (X117) EPT taxa decreases with stress 3 Percent Ephemeroptera the percent abundance of individuals in the sample that belong to the order Ephemeroptera (mayflies) The best standard value (BSV) is 477 Score = 100 x (X477) Percent Ephemeroptera decreases with stress 4 Percent Plecoptera + Trichoptera less Hydropsychidae the percent abundance of individuals in the sample that belong to the orders Plecoptera (stoneflies) and Trichoptera (caddisflies) excluding the pollution-tolerant caddisfly family Hydropsychidae The best standard value (BSV) is 562 Score = 100 x (X562) Percent Plecoptera + Trichoptera less Hydropsychidae decreases with stress 5 Percent Scrapers the percent abundance of individuals in the sample whose primary functional mechanism for feeding is grazing on the substrate or periphyton (ie attached algae and associated material) The best standard value (BSV) is 467 Score = 100 x (X467) Percent scrapers decreases with stress 6 Percent Chironomidae the percent abundance of individuals in the sample that are Chironomidae (non-biting midge) larvae and pupae The best standard value (BSV) is 16 Score = 100 x [(100-X)(100-16)] Percent Chironomidae increases with stress 7 Percent Top Two Dominant the percent abundance of the two most frequent taxa present in the sample The best standard value (BSV) is 328 Score = 100 x [(100-X)(100-328)] The Percent Top 2 Dominant increases with stress 8 Hilsenhoff Biotic Index (HBI) an abundance-weighted average pollution tolerance of all the individuals within a sample keyed to the family level Tolerance ratings range from 1 (highly intolerant) to 10 (highly tolerant) The best standard value (BSV) is 29 Score = 100 x [(10-X)(10-29)] The Hilsenhoff Biotic Index (HBI) increases with stress ______________________________________________________________________________ Table 13 Description of Benthic Macroinvertebrate VSCI Metrics Includes responses (increases decreases) of each VSCI metric to degraded water quality conditions Adapted from Burton and Gerritsen (2003 pp 3-15 3-16 6-2) and DEQ (2008)
q
Appendix C
LABORATORY BENCH SHEETS
Shenandoah Universityrsquos Environmental Studies Department developed the Benthic Macroinvertebrate Laboratory Bench Sheet and VSCI Worksheet to record BMI specimen identifications and their VSCI classifications for each sample
This form is based in part on bench sheets developed by the EPA and Virginia DEQ for recording specimen identifications Additional columns on Shenandoah Universityrsquos forms for relative numbers and VSCI classifications make the calculation and verification of VSCI scores easier
Note the column for taxonomistrsquos initials Specimens were identified by students in Shenandoah Universityrsquos research group and then verified or where necessary changed by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality This external review helps assure the validity of the study Originals of these bench sheets with handwritten taxonomistsrsquo initials will be kept on file at Shenandoah Universityrsquos Environmental Studies Department for five years after the completion of this study
r
s
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-1 STATION NAME Mouth LAT N 39deg 12749rsquo (upstream) LONG W 078deg 04209rsquo (upstream) LAT N 39deg 12729rsquo (downstream) LONG W 078deg 04148rsquo (downstream) FORM COMPILED BY Jeremy Tovar W S Bousquet RIVER BASIN Potomac River INVESTIGATORS Marie Dahl Brandon Millholand J W Pangle Sean Robertson Jeremy Tovar Woodward Bousquet Ben Bousquet
Collection date 21 May 2008 Sheet completion date 12 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Hirudinea 3 33 JT BVW PR 8 SP Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Coenagrionidae 2 22 BVW PR 9 CR Ephemeroptera (mayflies) Caenidae 1 11 JT BVW CG 6 SP Ephemerllidae 4 44 JT BVW CG 2 CR Heptageniidae 5 55 JT BVW SC 3 CG Isonychiidae 1 11 BVW CF 3 CG Plecoptera (stoneflies) Perlidae 14 154 JT BVW PR 2 CR Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Corydalidae 1 11 JT BVW PR 5 CR Coleoptera (beetles) Elmidae 24 264 I JT BVW SC 4 CG Dytiscidae 4 44 JT BVW PR 6 GN Psephenidae 3 33 JT BVW SC 4 CG Diptera (true flies) Tipulidae 3 33 JT BVW SH 4 BU Chironomidae 26 286 JT BVW CG 6 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
t
Other Total Individuals 91 1001 Total Taxa 13
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
u
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-1 STATION NAME Mouth LAT N 39deg 12749rsquo (upstream) LONG W 078deg 04209rsquo (upstream) LAT N 39deg 12729rsquo (downstream) LONG W 078deg 04148rsquo (downstream) FORM COMPILED BY Jeremy Tovar W S Bousquet RIVER BASIN Potomac River INVESTIGATORS Brandon Millholand JW Pangle Jeremy Tovar Heather Kepple
Collection date 10 October 2008 Sheet completion date 12 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Oligochaeta 3 35 JT BVW CG 8 BU Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Coenagrionidae 2 23 JT BVW PR 9 CR Ephemeroptera (mayflies) Heptageniidae 12 140 JT BVW SC 3 CG Isonychiidae 2 23 BVW CF 3 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Hydropsychidae 16 186 JT BVW CF 6 CG Philopotamidae 31 360 JT BVW CF 2 CG Hemiptera (true bugs) (Veliidae) (5) - JT BVW ( ) Megaloptera (dobsonflies etc) Corydalidae 10 116 JT BVW PR 5 CR Coleoptera (beetles) Dytiscidae 1 12 JT BVW PR 6 GN Psephenidae 7 81 JT BVW SC 4 CG Diptera (true flies) Tipulidae 2 23 BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
v
Other Total Individuals 86
(91) 999
Total Taxa 10 (11)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009 Veliidae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
w
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-2 STATION NAME Package Plant LAT N 39deg 12958rsquo (upstream) LONG W 078deg 04900 (upstream) LAT N 39deg 12958rsquo (downstream) LONG W 078deg 04822 (downstream) FORM COMPILED BY JW Pangle W Bousquet RIVER BASIN Potomac River INVESTIGATORS JW Pangle S Robertson J Tovar M Dahl B Millholland W Bousquet
Collection date 29 May 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Plecoptera (stoneflies) Perlidae 11 136 I JP BVW PR 1 CR Trichoptera (caddisflies) Hydropsychidae 10 123 I JP BVW CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 14 173 I JP BVW SC 4 CG Diptera (true flies) Chironomidae 32 395 I JP BVW CG 6 BU Simuliidae 14 173 I JP BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
x
Other Total Individuals 81 1000 Total Taxa 5
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-2 STATION NAME Package Plant LAT N 39deg 12958rsquo (upstream) LONG W 078deg 04900 (upstream) LAT N 39deg 12958rsquo (downstream) LONG W 078deg 04822 (downstream) FORM COMPILED BY JW Pangle W Bousquet RIVER BASIN Potomac River INVESTIGATORS JW Pangle Brandon Millholland Jeremy Tovar Heather Kepple
Collection date 10 October 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Heptageniidae 40 392 I WBJP SC 3 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Hydropsychidae 7 69 I JP WB BVW CF 6 CG Philopotamidae 28 275 I JP WB BVW CF 2 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Corydalidae 6 59 I JP WB BVW PR 5 CR Coleoptera (beetles) Psephenidae 7 69 I JP WB BVW SC 4 CG Elmidae 8 78 I JP WB BVW SC 4 CG Diptera (true flies) Tipulidae 6 59 I JP WB BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
y
z
Other Total Individuals 102 1001 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-3 STATION NAME Rt 664 Bridge LAT N 39deg 12934rsquo (upstream) LONG W 078deg 05019rsquo (upstream) LAT N 39deg 12951rsquo (downstream) LONG W 078deg 04958rsquo (downstream) FORM COMPILED BY S Robertson W Bousquet RIVER BASIN Potomac River INVESTIGATORS SRobertson BMillholland WBousquet MDahl JPangle
Collection date 28 May 2008 Sheet completion date 11 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Leptophlebiidae 1 15 I SGR BVW CG 2 CR Baetidae 1 15 I BVW CG 5 CG Plecoptera (stoneflies) Perlidae 3 46 I SGR BVW PR 1 CR Nemouridae 1 15 I BVW SH 3 CR Trichoptera (caddisflies) Hydropsychidae 5 77 I SGR BVW CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 27 415 I SGR BVW SC 4 CG Psephenidae 2 31 A SGR BVW SC 4 CG Diptera (true flies) Simuliidae 5 77 I SGR BVW CF 6 CG Chironomidae 20 308 I SGR BVW CG 6 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
aa
bb
Other Total Individuals 65 999 Total Taxa 9
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-3 STATION NAME Rt 664 Bridge LAT N 39deg 12934rsquo (upstream) LONG W 078deg 05019rsquo (upstream) LAT N 39deg 12951rsquo (downstream) LONG W 078deg 04958rsquo (downstream) FORM COMPILED BY S Robertson RIVER BASIN Potomac River INVESTIGATORS S Robertson M Dahl A Fisher H Kepple
Collection date 10 Oct 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Heptageniidae 2 20 I SGR SC 3 CG Isonychiidae 2 20 I BVW CF 2 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Philopotamidae 36 367 I SGR CF 2 CG Hydropsychidae 47 480 I SGR CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 2 20 A SGR SC 4 CG Psephenidae 8 82 A SGR SC 4 CG Diptera (true flies) Tabanidae 1 10 I SGR PR 7 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
cc
dd
Other Total Individuals 98 999 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-4 STATION NAME Unnamed Tributary LAT (upstream) N 39deg 13035rsquo LONG (upstream) W 078deg 05129rsquo LAT (downstream) N 39deg 13000rsquo LONG (downstream) W 078deg 5086rsquo FORM COMPILED BY A Fisher M Dahl rev W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson A Fisher H Kepple
Collection date 31 May 2008 Sheet completion date rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Flatworms (Turbellaria) Worms (Oligochaeta) Naididae 1 14 - BVW CG 8 BU Leeches (Hirudinea) Snails (Gastropoda) Clams (Bivalvia) Isopods (Isopoda) Asellidae 3 42 A AF BVW CG 8 SP Scuds (Amphipoda) Crayfish Shrimp (Decapoda) Water Mites (Hydracarina) Dragonflies (Odonata Anisoptera) Damselflies (Odonata Zygoptera) Mayflies (Ephemeroptera) Stoneflies (Plecoptera) Perlidae 2 28 I AF BVW PR 1 CR Caddisflies (Trichoptera) True Bugs (Hemiptera) Corixidae 1 14 I AF BVW MP 9 DV Dobsonflies (Megaloptera) Beetles (Coleoptera) Dytiscidae 1 14 I AF BVW PR 6 GN True Flies (Diptera) Chironomidae 61 847 I AF BVW CG 6 BU Simuliidae 3 42 I AF BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
ee
ff
Other Total Individuals 72 1001 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-4 STATION NAME Unnamed Tributary LAT (upstream) N 39deg 13035rsquo LONG (upstream) W 078deg 05129rsquo LAT (downstream) N 39deg 13000rsquo LONG (downstream) W 078deg 5086rsquo FORM COMPILED BY Andrew G M Fisher W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson A Fisher H Kepple
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Flatworms (Turbellaria) Worms (Oligochaeta) Lumbriculidae 2 32 A AF BVW CG 8 BU Leeches (Hirudinea) Snails (Gastropoda) Lymnaeidae 1 16 A BVW CG 9 GN Clams (Bivalvia) Isopods (Isopoda) Scuds (Amphipoda) Crayfish Shrimp (Decapoda) Cambaridae 1 16 A AF BVW GN 5 GN Water Mites (Hydracarina) Dragonflies (Odonata Anisoptera) Damselflies (Odonata Zygoptera) Calopterygidae 4 63 I BVW PR 7 CL Mayflies (Ephemeroptera) Tricorythidae 1 16 I BVW CG 5 SP Stoneflies (Plecoptera) Caddisflies (Trichoptera) Phryganeidae 4 63 I AF BVW SH 4 CR Hydropsychidae 6 95 I AF BVW CF 6 CG I True Bugs (Hemiptera) Corixidae 8 127 I AF BVW MP 9 DV (Veliidae ) (7) - I AF BVW ( ) Dobsonflies (Megaloptera) Sialidae 3 48 I AF BVW PR 7 SP Corydalidae 2 32 I AF BVW PR 5 CR Beetles (Coleoptera) Haliplidae 1 16 A AF BVW MP 8 GN Dysticidae 7 111 I AF BVW PR 6 GN Hydrophilidae 1 16 A AF BVW PR 8 GN Elmidae 3 48 I BVW SC 4 CG True Flies (Diptera) Chironomidae 8 127 I AF BVW CG 6 BU Simuliidae 7 111 I AF BVW CF 6 CG Tipulidae 4 63 I AF BVW SH 4 GU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
gg
hh
Other Total Individuals 63
(70) 1000
Total Taxa 17 (18)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Veliidae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-5 STATION NAME Lick Run LAT N 39deg 12752rsquo (upstream) LONG W 078deg 05685rsquo (upstream) LAT N 39deg 12784rsquo (downstream) LONG W 078deg 05631rsquo (downstream) FORM COMPILED BY Brandon Millholland W Bousquet RIVER BASIN Potomac River INVESTIGATORS J Pangle S Robertson W Bousquet JTovar M Dahl
Collection date 29 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Gomphidae 1 15 I BVW PR 3 BU Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Leptophlebiidae 11 167 A LBM BVW CG 2 CR Plecoptera (stoneflies) Perlidae 10 152 A LBM BVW PR 1 CR Perlodiae 1 15 A LBM BVW PR 2 CR Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 5 76 A LBM BVW SC 4 CG Elmidae 2 30 I LBM BVW SC 4 CG Diptera (true flies) (Culicidae) (3) - P LMB BVW ( ) Chironomidae 23 348 I LBM BVW CG 6 BU Simuliidae 13 197 I LBM BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
ii
jj
Other Total Individuals 66
(69) 1000
Total Taxa 7 (8)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Culicidae (Diptera) pupae not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl J Pangle S Robertson W Bousquet B Millholland J Tovar H Kepple
Collection date 30 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 1 12 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Asellidae 114 965 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Plecoptera (stoneflies) Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 2 23 A MRD BVW SC 4 CG Diptera (true flies)
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
kk
ll
Other Total Individuals 117 1000 Total Taxa 3
Notes Subsample taken 29 October 2009 All taxonomic determinations by MRD were confirmed by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson K McCann A Fisher H Kepple A Barrett
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 2 17 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Sphaeriidae 2 17 I MRD BVW CF 8 BU Isopoda (isopods) Asellidae 99 853 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Calopterygidae 2 17 A MRD BVW PR 7 CL Ephemeroptera (mayflies) Leptophlebiidae 2 17 I MRD BVW CG 2 CR Heptageniidae 4 34 A MRD BVW SC 3 CG Plecoptera (stoneflies) Perlidae 1 09 A MRD BVW PR 1 CR Trichoptera (caddisflies) Hemiptera (true bugs) (Gerridae) (1) - A MRD BVW ( ) Corixidae 1 09 A MRD BVW PR 5 CR Megaloptera (dobsonflies etc) Coleoptera (beetles) Dryopidae 1 09 A MRD BVW SC 5 CG Diptera (true flies) Tipulidae 2 17 I BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
mm
nn
Other Total Individuals 116
(117) 999
Total Taxa 10 (11)
Notes Subsample taken 6 November 2008 Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009 Gerridae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
- Disclaimer ii
- Title Page iii
- Acknowledgements v
- Table of Contents vii
- SECTION 2 RESULTS DATA TABLES 7
- OVERVIEW AND FINDINGS
- Introduction
- RESULTS DATA TABLES
- REFERENCES
- FIELD DATA SHEETS
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- SubstrateType
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- WATER QUALITY ANALYSIS
- THROUGH THE VIRGINIA
- STREAM CONDITION INDEX (VSCI)
- LABORATORY BENCH SHEETS
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Unnamed Tributary STATION HLR-4 STREAM CLASS LAT N 39 13035 (upstream) LONG W 078 05129 (upstream) LAT N 39 13000 (downstream) LONG W 078 05086 (downstream) FORM COMPILED BY Marie Dahl RIVER BASIN Potomac River INVESTIGATORS M Dahl HKepple SRobertson AFisher
DATE 31 May 2008 TIME 5 PM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality 2008
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) YES x NO
rain (steady rain) Air Temperature __22___degC showers (intermittent) Comments _______________________
_0__ cloud cover _0__ ______________________________
x clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area __-___ km2 mi2 Montane Significant springs andor seeps x Valley Marsh or swamp Montane amp Valley Mixture of origins Other ___________________
COMMENTS Snowden Bridge development (upstream) and sewer line
i
j
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence x FieldPasture Industrial Obvious sources Agricultural Other ____________ Construction upstream and adjacent x Residential Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left Forested with rocky banks field across silt fence and 2m tree strip Right Forested with some rocky banks and forest through the highway Indicate the dominant vegetation type(s) and record the dominant species
x Trees x Shrubs x Grasses Forbs Dominant species White Ash and Hackberry
INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width ___38__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area __380__m2
Estimated stream depth __12__cm Proportion of Reach Represented by Stream High water mark __2___m Morphology Types Surface velocity (at thalweg) __46___msec x Riffle__25___ x Run__70___ x Pool__5___ Channelized Yes x No Dam Present x Yes No
LARGE WOODY DEBRIS
LWD _______ m2 Density of LWD _______ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation ___NA____ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present___Algae___
WATER QUALITY Temperature __16___degC Water Odors x NormalNone Sewage Specific Conductance __-____ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ___-_____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used ____NA_____ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells x Other___Shale_______ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 0 Detritus sticks wood coarse plant materials
10 Boulder gt256 mm (10rsquorsquo) 5
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 45 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 30 Sand 006-2 mm (gritty) 0 Marl grey shell
fragments 0
Silt 0004-006 mm 20 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Lick Run STATION HLR-5 STREAM CLASS LAT N 39 127517 (upstream) LONG W 078 05685(upstream) LAT N 39 12784 (downstream) LONG W 078 05631 (downstream) FORM COMPILED BY B Millholland RIVER BASIN Potomac River INVESTIGATORS B Millholland JTovar SRobertson MDahl WBousquet JPangle
DATE 29 May 2008 TIME 1040 AM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project
k
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature __15___degC showers (intermittent) Comments _______________________
_5_ X cloud cover _0__ ______________________________
x clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane Significant springs andor seeps x Valley Marsh or swamp Montane amp Valley Mixture of origins Other ___________________
COMMENTS Lick Run appears to be intermittent and may be dry in Fall 2008
l
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence x FieldPasture Industrial Obvious sources Agricultural Other ____________ Adjacent logging stream bank scour Residential Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left 85 young deciduous forest 15 logged Bank=Soil vegetation and cobbles Right Young deciduous forest on 30 slope Bank= Soil vegetation and cobble Indicate the dominant vegetation type(s) and record the dominant species
x Trees Shrubs Grasses Forbs Dominant species Mixed Deciduous INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width __11__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area 110 m2
Estimated stream depth _009__m Proportion of Reach Represented by Stream High water mark __96___m Morphology Types Surface velocity (at thalweg) __01___msec x Riffle__30___ x Run__5___ x Pool__65___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___0____ m2 Density of LWD ___-____ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __70___ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present__________________________________________________________
WATER QUALITY Temperature __14___degC Water Odors x NormalNone Sewage Specific Conductance __-__ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ___-___ Water Surface Oils Slick Sheen Globs Flecks pH __-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used _____NA______ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other_____None_____
SEDIMENT AND SUBSTRATE
Odors Deposits Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other________________ x Other Slightly Salty Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 0 Detritus sticks wood coarse plant materials
5 Boulder gt256 mm (10rsquorsquo) 0
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 75 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 23 Sand 006-2 mm (gritty) 2 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION McCann Farm STATION HLR-6 STREAM CLASS LAT N 39 13076 (upstream) LONG W 078 06539 (upstream) LAT N 39 13063 (downstream) LONG W 078 06473 (downstream) FORM COMPILED BY MDahl RIVER BASIN Potomac River INVESTIGATORS MDahl JPangle SRobertson W Bousqet BMillholland JTovar H Kepple
DATE 30 May 2008 TIME 1050 AM (start)
PROJECT NAME
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) YES x NO
rain (steady rain) Air Temperature __18___degC showers (intermittent) Comments _______________________
_85_x cloud cover _10__ ______________________________
clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x (Significant springs andor seeps) x ( Valley) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS Multi-habitat sampling with D-frame nets and seine
m
n
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial x No evidence Some evidence x FieldPasture Industrial Obvious sources x Agricultural Other ____________ Minor stream bank scour Residential Local Watershed Erosion x None Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left 35 bank grasses scattered trees and some bushes Right High bank with grasses and scattered trees Indicate the dominant vegetation type(s) and record the dominant species
x Trees Shrubs Grasses Forbs Dominant species Black Walnut and Festuca elatior
INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width __2___m Fully open Partly open x Partly shaded Fully shaded Sampling reach area __200_m2
Estimated stream depth __03_m Proportion of Reach Represented by Stream High water mark __1___m Morphology Types Surface velocity (at thalweg) __166_msec x Riffle__0___ x Run__55___ x Pool__45___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___2____ m2 Density of LWD ___-____ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __20_____ x Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present ____Glyceria (grass)sp__________
WATER QUALITY Temperature __16___degC Water Odors x NormalNone Sewage Specific Conductance ___-___ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ____-____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used ______NA___ Clear x Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other__NONE__ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 0 Detritus sticks wood coarse plant materials
5 Boulder gt256 mm (10rsquorsquo) 0
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 27 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 38 Sand 006-2 mm (gritty) 5 Marl grey shell
fragments 0 (lt1)
Silt 0004-006 mm 25 Clay lt 0004 mm (slick) 5 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Appendix B
WATER QUALITY ANALYSIS THROUGH THE VIRGINIA
STREAM CONDITION INDEX (VSCI)
The Virginia Stream Condition Index (VSCI) relies on eight metrics to categorize stream health According to Burton and Gerritsen (2003 p 1-1) this index ldquohellip can be used as a primary indicator of ecosystem health and can identify impairment with respect to the reference (or natural) conditionrdquo These metrics are numeric measures of a biological communityrsquos species composition diversity ecological niches or tolerance to pollution
Table 13 on the next page describes each VSCI metric lists its formula and provides the best standard value (BSV) for that metric Based on a 100-point scale the BSV is the score that reflects optimum conditions for aquatic life ie the best water quality value possible (DEQ 2006b pp 8 33) The table also explains whether the score increases or decreases with environmental stress
As Table 13 indicates each of the eight VSCI metrics is calculated individually Each formula provides a unitless score based on a 100-point scale with 0 as the worst possible score and 100 as the best Any metric that provides a result above 100 is changed to 100 in order for the index to work properly After the eight metrics are calculated they are summed and averaged to provide a final VSCI score on a 100-point scale
Based on statistical analysis of Virginiarsquos streams and their BMI communities the DEQ (2006b p 8) currently uses the following tiers to interpret VSCI scores
VSCI Scores Aquatic Life Use
(ALI) Tiers
lt 42 severely stressed
42-55 moderately stressed
55-63 unknownfair (gray zone)
63-73 good
gt73 excellent
Section 2 of this report presents the results of calculations for the six BMI sampling sites the research team studied in the Hiatt-Lick Run subwatershed in 2008
o
p
1 Total taxa the number of distinct taxa in the sample measures the overall diversity of the macroinvertebrate assemblage The best standard value (BSV) is 189 Score = 100 x (X189) Total taxa decreases with stress 2 EPT taxa the sum of the distinct taxa in the orders Ephemeroptera (mayflies) Plecoptera (stoneflies) and Trichoptera (caddisflies) These orders are known to be sensitive to pollution The best standard value (BSV) is 117 Score = 100 x (X117) EPT taxa decreases with stress 3 Percent Ephemeroptera the percent abundance of individuals in the sample that belong to the order Ephemeroptera (mayflies) The best standard value (BSV) is 477 Score = 100 x (X477) Percent Ephemeroptera decreases with stress 4 Percent Plecoptera + Trichoptera less Hydropsychidae the percent abundance of individuals in the sample that belong to the orders Plecoptera (stoneflies) and Trichoptera (caddisflies) excluding the pollution-tolerant caddisfly family Hydropsychidae The best standard value (BSV) is 562 Score = 100 x (X562) Percent Plecoptera + Trichoptera less Hydropsychidae decreases with stress 5 Percent Scrapers the percent abundance of individuals in the sample whose primary functional mechanism for feeding is grazing on the substrate or periphyton (ie attached algae and associated material) The best standard value (BSV) is 467 Score = 100 x (X467) Percent scrapers decreases with stress 6 Percent Chironomidae the percent abundance of individuals in the sample that are Chironomidae (non-biting midge) larvae and pupae The best standard value (BSV) is 16 Score = 100 x [(100-X)(100-16)] Percent Chironomidae increases with stress 7 Percent Top Two Dominant the percent abundance of the two most frequent taxa present in the sample The best standard value (BSV) is 328 Score = 100 x [(100-X)(100-328)] The Percent Top 2 Dominant increases with stress 8 Hilsenhoff Biotic Index (HBI) an abundance-weighted average pollution tolerance of all the individuals within a sample keyed to the family level Tolerance ratings range from 1 (highly intolerant) to 10 (highly tolerant) The best standard value (BSV) is 29 Score = 100 x [(10-X)(10-29)] The Hilsenhoff Biotic Index (HBI) increases with stress ______________________________________________________________________________ Table 13 Description of Benthic Macroinvertebrate VSCI Metrics Includes responses (increases decreases) of each VSCI metric to degraded water quality conditions Adapted from Burton and Gerritsen (2003 pp 3-15 3-16 6-2) and DEQ (2008)
q
Appendix C
LABORATORY BENCH SHEETS
Shenandoah Universityrsquos Environmental Studies Department developed the Benthic Macroinvertebrate Laboratory Bench Sheet and VSCI Worksheet to record BMI specimen identifications and their VSCI classifications for each sample
This form is based in part on bench sheets developed by the EPA and Virginia DEQ for recording specimen identifications Additional columns on Shenandoah Universityrsquos forms for relative numbers and VSCI classifications make the calculation and verification of VSCI scores easier
Note the column for taxonomistrsquos initials Specimens were identified by students in Shenandoah Universityrsquos research group and then verified or where necessary changed by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality This external review helps assure the validity of the study Originals of these bench sheets with handwritten taxonomistsrsquo initials will be kept on file at Shenandoah Universityrsquos Environmental Studies Department for five years after the completion of this study
r
s
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-1 STATION NAME Mouth LAT N 39deg 12749rsquo (upstream) LONG W 078deg 04209rsquo (upstream) LAT N 39deg 12729rsquo (downstream) LONG W 078deg 04148rsquo (downstream) FORM COMPILED BY Jeremy Tovar W S Bousquet RIVER BASIN Potomac River INVESTIGATORS Marie Dahl Brandon Millholand J W Pangle Sean Robertson Jeremy Tovar Woodward Bousquet Ben Bousquet
Collection date 21 May 2008 Sheet completion date 12 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Hirudinea 3 33 JT BVW PR 8 SP Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Coenagrionidae 2 22 BVW PR 9 CR Ephemeroptera (mayflies) Caenidae 1 11 JT BVW CG 6 SP Ephemerllidae 4 44 JT BVW CG 2 CR Heptageniidae 5 55 JT BVW SC 3 CG Isonychiidae 1 11 BVW CF 3 CG Plecoptera (stoneflies) Perlidae 14 154 JT BVW PR 2 CR Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Corydalidae 1 11 JT BVW PR 5 CR Coleoptera (beetles) Elmidae 24 264 I JT BVW SC 4 CG Dytiscidae 4 44 JT BVW PR 6 GN Psephenidae 3 33 JT BVW SC 4 CG Diptera (true flies) Tipulidae 3 33 JT BVW SH 4 BU Chironomidae 26 286 JT BVW CG 6 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
t
Other Total Individuals 91 1001 Total Taxa 13
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
u
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-1 STATION NAME Mouth LAT N 39deg 12749rsquo (upstream) LONG W 078deg 04209rsquo (upstream) LAT N 39deg 12729rsquo (downstream) LONG W 078deg 04148rsquo (downstream) FORM COMPILED BY Jeremy Tovar W S Bousquet RIVER BASIN Potomac River INVESTIGATORS Brandon Millholand JW Pangle Jeremy Tovar Heather Kepple
Collection date 10 October 2008 Sheet completion date 12 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Oligochaeta 3 35 JT BVW CG 8 BU Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Coenagrionidae 2 23 JT BVW PR 9 CR Ephemeroptera (mayflies) Heptageniidae 12 140 JT BVW SC 3 CG Isonychiidae 2 23 BVW CF 3 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Hydropsychidae 16 186 JT BVW CF 6 CG Philopotamidae 31 360 JT BVW CF 2 CG Hemiptera (true bugs) (Veliidae) (5) - JT BVW ( ) Megaloptera (dobsonflies etc) Corydalidae 10 116 JT BVW PR 5 CR Coleoptera (beetles) Dytiscidae 1 12 JT BVW PR 6 GN Psephenidae 7 81 JT BVW SC 4 CG Diptera (true flies) Tipulidae 2 23 BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
v
Other Total Individuals 86
(91) 999
Total Taxa 10 (11)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009 Veliidae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
w
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-2 STATION NAME Package Plant LAT N 39deg 12958rsquo (upstream) LONG W 078deg 04900 (upstream) LAT N 39deg 12958rsquo (downstream) LONG W 078deg 04822 (downstream) FORM COMPILED BY JW Pangle W Bousquet RIVER BASIN Potomac River INVESTIGATORS JW Pangle S Robertson J Tovar M Dahl B Millholland W Bousquet
Collection date 29 May 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Plecoptera (stoneflies) Perlidae 11 136 I JP BVW PR 1 CR Trichoptera (caddisflies) Hydropsychidae 10 123 I JP BVW CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 14 173 I JP BVW SC 4 CG Diptera (true flies) Chironomidae 32 395 I JP BVW CG 6 BU Simuliidae 14 173 I JP BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
x
Other Total Individuals 81 1000 Total Taxa 5
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-2 STATION NAME Package Plant LAT N 39deg 12958rsquo (upstream) LONG W 078deg 04900 (upstream) LAT N 39deg 12958rsquo (downstream) LONG W 078deg 04822 (downstream) FORM COMPILED BY JW Pangle W Bousquet RIVER BASIN Potomac River INVESTIGATORS JW Pangle Brandon Millholland Jeremy Tovar Heather Kepple
Collection date 10 October 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Heptageniidae 40 392 I WBJP SC 3 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Hydropsychidae 7 69 I JP WB BVW CF 6 CG Philopotamidae 28 275 I JP WB BVW CF 2 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Corydalidae 6 59 I JP WB BVW PR 5 CR Coleoptera (beetles) Psephenidae 7 69 I JP WB BVW SC 4 CG Elmidae 8 78 I JP WB BVW SC 4 CG Diptera (true flies) Tipulidae 6 59 I JP WB BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
y
z
Other Total Individuals 102 1001 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-3 STATION NAME Rt 664 Bridge LAT N 39deg 12934rsquo (upstream) LONG W 078deg 05019rsquo (upstream) LAT N 39deg 12951rsquo (downstream) LONG W 078deg 04958rsquo (downstream) FORM COMPILED BY S Robertson W Bousquet RIVER BASIN Potomac River INVESTIGATORS SRobertson BMillholland WBousquet MDahl JPangle
Collection date 28 May 2008 Sheet completion date 11 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Leptophlebiidae 1 15 I SGR BVW CG 2 CR Baetidae 1 15 I BVW CG 5 CG Plecoptera (stoneflies) Perlidae 3 46 I SGR BVW PR 1 CR Nemouridae 1 15 I BVW SH 3 CR Trichoptera (caddisflies) Hydropsychidae 5 77 I SGR BVW CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 27 415 I SGR BVW SC 4 CG Psephenidae 2 31 A SGR BVW SC 4 CG Diptera (true flies) Simuliidae 5 77 I SGR BVW CF 6 CG Chironomidae 20 308 I SGR BVW CG 6 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
aa
bb
Other Total Individuals 65 999 Total Taxa 9
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-3 STATION NAME Rt 664 Bridge LAT N 39deg 12934rsquo (upstream) LONG W 078deg 05019rsquo (upstream) LAT N 39deg 12951rsquo (downstream) LONG W 078deg 04958rsquo (downstream) FORM COMPILED BY S Robertson RIVER BASIN Potomac River INVESTIGATORS S Robertson M Dahl A Fisher H Kepple
Collection date 10 Oct 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Heptageniidae 2 20 I SGR SC 3 CG Isonychiidae 2 20 I BVW CF 2 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Philopotamidae 36 367 I SGR CF 2 CG Hydropsychidae 47 480 I SGR CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 2 20 A SGR SC 4 CG Psephenidae 8 82 A SGR SC 4 CG Diptera (true flies) Tabanidae 1 10 I SGR PR 7 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
cc
dd
Other Total Individuals 98 999 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-4 STATION NAME Unnamed Tributary LAT (upstream) N 39deg 13035rsquo LONG (upstream) W 078deg 05129rsquo LAT (downstream) N 39deg 13000rsquo LONG (downstream) W 078deg 5086rsquo FORM COMPILED BY A Fisher M Dahl rev W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson A Fisher H Kepple
Collection date 31 May 2008 Sheet completion date rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Flatworms (Turbellaria) Worms (Oligochaeta) Naididae 1 14 - BVW CG 8 BU Leeches (Hirudinea) Snails (Gastropoda) Clams (Bivalvia) Isopods (Isopoda) Asellidae 3 42 A AF BVW CG 8 SP Scuds (Amphipoda) Crayfish Shrimp (Decapoda) Water Mites (Hydracarina) Dragonflies (Odonata Anisoptera) Damselflies (Odonata Zygoptera) Mayflies (Ephemeroptera) Stoneflies (Plecoptera) Perlidae 2 28 I AF BVW PR 1 CR Caddisflies (Trichoptera) True Bugs (Hemiptera) Corixidae 1 14 I AF BVW MP 9 DV Dobsonflies (Megaloptera) Beetles (Coleoptera) Dytiscidae 1 14 I AF BVW PR 6 GN True Flies (Diptera) Chironomidae 61 847 I AF BVW CG 6 BU Simuliidae 3 42 I AF BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
ee
ff
Other Total Individuals 72 1001 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-4 STATION NAME Unnamed Tributary LAT (upstream) N 39deg 13035rsquo LONG (upstream) W 078deg 05129rsquo LAT (downstream) N 39deg 13000rsquo LONG (downstream) W 078deg 5086rsquo FORM COMPILED BY Andrew G M Fisher W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson A Fisher H Kepple
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Flatworms (Turbellaria) Worms (Oligochaeta) Lumbriculidae 2 32 A AF BVW CG 8 BU Leeches (Hirudinea) Snails (Gastropoda) Lymnaeidae 1 16 A BVW CG 9 GN Clams (Bivalvia) Isopods (Isopoda) Scuds (Amphipoda) Crayfish Shrimp (Decapoda) Cambaridae 1 16 A AF BVW GN 5 GN Water Mites (Hydracarina) Dragonflies (Odonata Anisoptera) Damselflies (Odonata Zygoptera) Calopterygidae 4 63 I BVW PR 7 CL Mayflies (Ephemeroptera) Tricorythidae 1 16 I BVW CG 5 SP Stoneflies (Plecoptera) Caddisflies (Trichoptera) Phryganeidae 4 63 I AF BVW SH 4 CR Hydropsychidae 6 95 I AF BVW CF 6 CG I True Bugs (Hemiptera) Corixidae 8 127 I AF BVW MP 9 DV (Veliidae ) (7) - I AF BVW ( ) Dobsonflies (Megaloptera) Sialidae 3 48 I AF BVW PR 7 SP Corydalidae 2 32 I AF BVW PR 5 CR Beetles (Coleoptera) Haliplidae 1 16 A AF BVW MP 8 GN Dysticidae 7 111 I AF BVW PR 6 GN Hydrophilidae 1 16 A AF BVW PR 8 GN Elmidae 3 48 I BVW SC 4 CG True Flies (Diptera) Chironomidae 8 127 I AF BVW CG 6 BU Simuliidae 7 111 I AF BVW CF 6 CG Tipulidae 4 63 I AF BVW SH 4 GU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
gg
hh
Other Total Individuals 63
(70) 1000
Total Taxa 17 (18)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Veliidae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-5 STATION NAME Lick Run LAT N 39deg 12752rsquo (upstream) LONG W 078deg 05685rsquo (upstream) LAT N 39deg 12784rsquo (downstream) LONG W 078deg 05631rsquo (downstream) FORM COMPILED BY Brandon Millholland W Bousquet RIVER BASIN Potomac River INVESTIGATORS J Pangle S Robertson W Bousquet JTovar M Dahl
Collection date 29 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Gomphidae 1 15 I BVW PR 3 BU Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Leptophlebiidae 11 167 A LBM BVW CG 2 CR Plecoptera (stoneflies) Perlidae 10 152 A LBM BVW PR 1 CR Perlodiae 1 15 A LBM BVW PR 2 CR Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 5 76 A LBM BVW SC 4 CG Elmidae 2 30 I LBM BVW SC 4 CG Diptera (true flies) (Culicidae) (3) - P LMB BVW ( ) Chironomidae 23 348 I LBM BVW CG 6 BU Simuliidae 13 197 I LBM BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
ii
jj
Other Total Individuals 66
(69) 1000
Total Taxa 7 (8)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Culicidae (Diptera) pupae not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl J Pangle S Robertson W Bousquet B Millholland J Tovar H Kepple
Collection date 30 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 1 12 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Asellidae 114 965 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Plecoptera (stoneflies) Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 2 23 A MRD BVW SC 4 CG Diptera (true flies)
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
kk
ll
Other Total Individuals 117 1000 Total Taxa 3
Notes Subsample taken 29 October 2009 All taxonomic determinations by MRD were confirmed by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson K McCann A Fisher H Kepple A Barrett
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 2 17 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Sphaeriidae 2 17 I MRD BVW CF 8 BU Isopoda (isopods) Asellidae 99 853 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Calopterygidae 2 17 A MRD BVW PR 7 CL Ephemeroptera (mayflies) Leptophlebiidae 2 17 I MRD BVW CG 2 CR Heptageniidae 4 34 A MRD BVW SC 3 CG Plecoptera (stoneflies) Perlidae 1 09 A MRD BVW PR 1 CR Trichoptera (caddisflies) Hemiptera (true bugs) (Gerridae) (1) - A MRD BVW ( ) Corixidae 1 09 A MRD BVW PR 5 CR Megaloptera (dobsonflies etc) Coleoptera (beetles) Dryopidae 1 09 A MRD BVW SC 5 CG Diptera (true flies) Tipulidae 2 17 I BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
mm
nn
Other Total Individuals 116
(117) 999
Total Taxa 10 (11)
Notes Subsample taken 6 November 2008 Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009 Gerridae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
- Disclaimer ii
- Title Page iii
- Acknowledgements v
- Table of Contents vii
- SECTION 2 RESULTS DATA TABLES 7
- OVERVIEW AND FINDINGS
- Introduction
- RESULTS DATA TABLES
- REFERENCES
- FIELD DATA SHEETS
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- SubstrateType
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- WATER QUALITY ANALYSIS
- THROUGH THE VIRGINIA
- STREAM CONDITION INDEX (VSCI)
- LABORATORY BENCH SHEETS
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
j
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence x FieldPasture Industrial Obvious sources Agricultural Other ____________ Construction upstream and adjacent x Residential Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left Forested with rocky banks field across silt fence and 2m tree strip Right Forested with some rocky banks and forest through the highway Indicate the dominant vegetation type(s) and record the dominant species
x Trees x Shrubs x Grasses Forbs Dominant species White Ash and Hackberry
INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width ___38__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area __380__m2
Estimated stream depth __12__cm Proportion of Reach Represented by Stream High water mark __2___m Morphology Types Surface velocity (at thalweg) __46___msec x Riffle__25___ x Run__70___ x Pool__5___ Channelized Yes x No Dam Present x Yes No
LARGE WOODY DEBRIS
LWD _______ m2 Density of LWD _______ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation ___NA____ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present___Algae___
WATER QUALITY Temperature __16___degC Water Odors x NormalNone Sewage Specific Conductance __-____ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ___-_____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used ____NA_____ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells x Other___Shale_______ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 0 Detritus sticks wood coarse plant materials
10 Boulder gt256 mm (10rsquorsquo) 5
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 45 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 30 Sand 006-2 mm (gritty) 0 Marl grey shell
fragments 0
Silt 0004-006 mm 20 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Lick Run STATION HLR-5 STREAM CLASS LAT N 39 127517 (upstream) LONG W 078 05685(upstream) LAT N 39 12784 (downstream) LONG W 078 05631 (downstream) FORM COMPILED BY B Millholland RIVER BASIN Potomac River INVESTIGATORS B Millholland JTovar SRobertson MDahl WBousquet JPangle
DATE 29 May 2008 TIME 1040 AM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project
k
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature __15___degC showers (intermittent) Comments _______________________
_5_ X cloud cover _0__ ______________________________
x clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane Significant springs andor seeps x Valley Marsh or swamp Montane amp Valley Mixture of origins Other ___________________
COMMENTS Lick Run appears to be intermittent and may be dry in Fall 2008
l
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence x FieldPasture Industrial Obvious sources Agricultural Other ____________ Adjacent logging stream bank scour Residential Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left 85 young deciduous forest 15 logged Bank=Soil vegetation and cobbles Right Young deciduous forest on 30 slope Bank= Soil vegetation and cobble Indicate the dominant vegetation type(s) and record the dominant species
x Trees Shrubs Grasses Forbs Dominant species Mixed Deciduous INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width __11__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area 110 m2
Estimated stream depth _009__m Proportion of Reach Represented by Stream High water mark __96___m Morphology Types Surface velocity (at thalweg) __01___msec x Riffle__30___ x Run__5___ x Pool__65___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___0____ m2 Density of LWD ___-____ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __70___ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present__________________________________________________________
WATER QUALITY Temperature __14___degC Water Odors x NormalNone Sewage Specific Conductance __-__ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ___-___ Water Surface Oils Slick Sheen Globs Flecks pH __-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used _____NA______ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other_____None_____
SEDIMENT AND SUBSTRATE
Odors Deposits Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other________________ x Other Slightly Salty Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 0 Detritus sticks wood coarse plant materials
5 Boulder gt256 mm (10rsquorsquo) 0
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 75 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 23 Sand 006-2 mm (gritty) 2 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION McCann Farm STATION HLR-6 STREAM CLASS LAT N 39 13076 (upstream) LONG W 078 06539 (upstream) LAT N 39 13063 (downstream) LONG W 078 06473 (downstream) FORM COMPILED BY MDahl RIVER BASIN Potomac River INVESTIGATORS MDahl JPangle SRobertson W Bousqet BMillholland JTovar H Kepple
DATE 30 May 2008 TIME 1050 AM (start)
PROJECT NAME
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) YES x NO
rain (steady rain) Air Temperature __18___degC showers (intermittent) Comments _______________________
_85_x cloud cover _10__ ______________________________
clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x (Significant springs andor seeps) x ( Valley) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS Multi-habitat sampling with D-frame nets and seine
m
n
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial x No evidence Some evidence x FieldPasture Industrial Obvious sources x Agricultural Other ____________ Minor stream bank scour Residential Local Watershed Erosion x None Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left 35 bank grasses scattered trees and some bushes Right High bank with grasses and scattered trees Indicate the dominant vegetation type(s) and record the dominant species
x Trees Shrubs Grasses Forbs Dominant species Black Walnut and Festuca elatior
INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width __2___m Fully open Partly open x Partly shaded Fully shaded Sampling reach area __200_m2
Estimated stream depth __03_m Proportion of Reach Represented by Stream High water mark __1___m Morphology Types Surface velocity (at thalweg) __166_msec x Riffle__0___ x Run__55___ x Pool__45___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___2____ m2 Density of LWD ___-____ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __20_____ x Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present ____Glyceria (grass)sp__________
WATER QUALITY Temperature __16___degC Water Odors x NormalNone Sewage Specific Conductance ___-___ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ____-____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used ______NA___ Clear x Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other__NONE__ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 0 Detritus sticks wood coarse plant materials
5 Boulder gt256 mm (10rsquorsquo) 0
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 27 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 38 Sand 006-2 mm (gritty) 5 Marl grey shell
fragments 0 (lt1)
Silt 0004-006 mm 25 Clay lt 0004 mm (slick) 5 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Appendix B
WATER QUALITY ANALYSIS THROUGH THE VIRGINIA
STREAM CONDITION INDEX (VSCI)
The Virginia Stream Condition Index (VSCI) relies on eight metrics to categorize stream health According to Burton and Gerritsen (2003 p 1-1) this index ldquohellip can be used as a primary indicator of ecosystem health and can identify impairment with respect to the reference (or natural) conditionrdquo These metrics are numeric measures of a biological communityrsquos species composition diversity ecological niches or tolerance to pollution
Table 13 on the next page describes each VSCI metric lists its formula and provides the best standard value (BSV) for that metric Based on a 100-point scale the BSV is the score that reflects optimum conditions for aquatic life ie the best water quality value possible (DEQ 2006b pp 8 33) The table also explains whether the score increases or decreases with environmental stress
As Table 13 indicates each of the eight VSCI metrics is calculated individually Each formula provides a unitless score based on a 100-point scale with 0 as the worst possible score and 100 as the best Any metric that provides a result above 100 is changed to 100 in order for the index to work properly After the eight metrics are calculated they are summed and averaged to provide a final VSCI score on a 100-point scale
Based on statistical analysis of Virginiarsquos streams and their BMI communities the DEQ (2006b p 8) currently uses the following tiers to interpret VSCI scores
VSCI Scores Aquatic Life Use
(ALI) Tiers
lt 42 severely stressed
42-55 moderately stressed
55-63 unknownfair (gray zone)
63-73 good
gt73 excellent
Section 2 of this report presents the results of calculations for the six BMI sampling sites the research team studied in the Hiatt-Lick Run subwatershed in 2008
o
p
1 Total taxa the number of distinct taxa in the sample measures the overall diversity of the macroinvertebrate assemblage The best standard value (BSV) is 189 Score = 100 x (X189) Total taxa decreases with stress 2 EPT taxa the sum of the distinct taxa in the orders Ephemeroptera (mayflies) Plecoptera (stoneflies) and Trichoptera (caddisflies) These orders are known to be sensitive to pollution The best standard value (BSV) is 117 Score = 100 x (X117) EPT taxa decreases with stress 3 Percent Ephemeroptera the percent abundance of individuals in the sample that belong to the order Ephemeroptera (mayflies) The best standard value (BSV) is 477 Score = 100 x (X477) Percent Ephemeroptera decreases with stress 4 Percent Plecoptera + Trichoptera less Hydropsychidae the percent abundance of individuals in the sample that belong to the orders Plecoptera (stoneflies) and Trichoptera (caddisflies) excluding the pollution-tolerant caddisfly family Hydropsychidae The best standard value (BSV) is 562 Score = 100 x (X562) Percent Plecoptera + Trichoptera less Hydropsychidae decreases with stress 5 Percent Scrapers the percent abundance of individuals in the sample whose primary functional mechanism for feeding is grazing on the substrate or periphyton (ie attached algae and associated material) The best standard value (BSV) is 467 Score = 100 x (X467) Percent scrapers decreases with stress 6 Percent Chironomidae the percent abundance of individuals in the sample that are Chironomidae (non-biting midge) larvae and pupae The best standard value (BSV) is 16 Score = 100 x [(100-X)(100-16)] Percent Chironomidae increases with stress 7 Percent Top Two Dominant the percent abundance of the two most frequent taxa present in the sample The best standard value (BSV) is 328 Score = 100 x [(100-X)(100-328)] The Percent Top 2 Dominant increases with stress 8 Hilsenhoff Biotic Index (HBI) an abundance-weighted average pollution tolerance of all the individuals within a sample keyed to the family level Tolerance ratings range from 1 (highly intolerant) to 10 (highly tolerant) The best standard value (BSV) is 29 Score = 100 x [(10-X)(10-29)] The Hilsenhoff Biotic Index (HBI) increases with stress ______________________________________________________________________________ Table 13 Description of Benthic Macroinvertebrate VSCI Metrics Includes responses (increases decreases) of each VSCI metric to degraded water quality conditions Adapted from Burton and Gerritsen (2003 pp 3-15 3-16 6-2) and DEQ (2008)
q
Appendix C
LABORATORY BENCH SHEETS
Shenandoah Universityrsquos Environmental Studies Department developed the Benthic Macroinvertebrate Laboratory Bench Sheet and VSCI Worksheet to record BMI specimen identifications and their VSCI classifications for each sample
This form is based in part on bench sheets developed by the EPA and Virginia DEQ for recording specimen identifications Additional columns on Shenandoah Universityrsquos forms for relative numbers and VSCI classifications make the calculation and verification of VSCI scores easier
Note the column for taxonomistrsquos initials Specimens were identified by students in Shenandoah Universityrsquos research group and then verified or where necessary changed by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality This external review helps assure the validity of the study Originals of these bench sheets with handwritten taxonomistsrsquo initials will be kept on file at Shenandoah Universityrsquos Environmental Studies Department for five years after the completion of this study
r
s
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-1 STATION NAME Mouth LAT N 39deg 12749rsquo (upstream) LONG W 078deg 04209rsquo (upstream) LAT N 39deg 12729rsquo (downstream) LONG W 078deg 04148rsquo (downstream) FORM COMPILED BY Jeremy Tovar W S Bousquet RIVER BASIN Potomac River INVESTIGATORS Marie Dahl Brandon Millholand J W Pangle Sean Robertson Jeremy Tovar Woodward Bousquet Ben Bousquet
Collection date 21 May 2008 Sheet completion date 12 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Hirudinea 3 33 JT BVW PR 8 SP Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Coenagrionidae 2 22 BVW PR 9 CR Ephemeroptera (mayflies) Caenidae 1 11 JT BVW CG 6 SP Ephemerllidae 4 44 JT BVW CG 2 CR Heptageniidae 5 55 JT BVW SC 3 CG Isonychiidae 1 11 BVW CF 3 CG Plecoptera (stoneflies) Perlidae 14 154 JT BVW PR 2 CR Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Corydalidae 1 11 JT BVW PR 5 CR Coleoptera (beetles) Elmidae 24 264 I JT BVW SC 4 CG Dytiscidae 4 44 JT BVW PR 6 GN Psephenidae 3 33 JT BVW SC 4 CG Diptera (true flies) Tipulidae 3 33 JT BVW SH 4 BU Chironomidae 26 286 JT BVW CG 6 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
t
Other Total Individuals 91 1001 Total Taxa 13
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
u
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-1 STATION NAME Mouth LAT N 39deg 12749rsquo (upstream) LONG W 078deg 04209rsquo (upstream) LAT N 39deg 12729rsquo (downstream) LONG W 078deg 04148rsquo (downstream) FORM COMPILED BY Jeremy Tovar W S Bousquet RIVER BASIN Potomac River INVESTIGATORS Brandon Millholand JW Pangle Jeremy Tovar Heather Kepple
Collection date 10 October 2008 Sheet completion date 12 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Oligochaeta 3 35 JT BVW CG 8 BU Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Coenagrionidae 2 23 JT BVW PR 9 CR Ephemeroptera (mayflies) Heptageniidae 12 140 JT BVW SC 3 CG Isonychiidae 2 23 BVW CF 3 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Hydropsychidae 16 186 JT BVW CF 6 CG Philopotamidae 31 360 JT BVW CF 2 CG Hemiptera (true bugs) (Veliidae) (5) - JT BVW ( ) Megaloptera (dobsonflies etc) Corydalidae 10 116 JT BVW PR 5 CR Coleoptera (beetles) Dytiscidae 1 12 JT BVW PR 6 GN Psephenidae 7 81 JT BVW SC 4 CG Diptera (true flies) Tipulidae 2 23 BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
v
Other Total Individuals 86
(91) 999
Total Taxa 10 (11)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009 Veliidae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
w
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-2 STATION NAME Package Plant LAT N 39deg 12958rsquo (upstream) LONG W 078deg 04900 (upstream) LAT N 39deg 12958rsquo (downstream) LONG W 078deg 04822 (downstream) FORM COMPILED BY JW Pangle W Bousquet RIVER BASIN Potomac River INVESTIGATORS JW Pangle S Robertson J Tovar M Dahl B Millholland W Bousquet
Collection date 29 May 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Plecoptera (stoneflies) Perlidae 11 136 I JP BVW PR 1 CR Trichoptera (caddisflies) Hydropsychidae 10 123 I JP BVW CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 14 173 I JP BVW SC 4 CG Diptera (true flies) Chironomidae 32 395 I JP BVW CG 6 BU Simuliidae 14 173 I JP BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
x
Other Total Individuals 81 1000 Total Taxa 5
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-2 STATION NAME Package Plant LAT N 39deg 12958rsquo (upstream) LONG W 078deg 04900 (upstream) LAT N 39deg 12958rsquo (downstream) LONG W 078deg 04822 (downstream) FORM COMPILED BY JW Pangle W Bousquet RIVER BASIN Potomac River INVESTIGATORS JW Pangle Brandon Millholland Jeremy Tovar Heather Kepple
Collection date 10 October 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Heptageniidae 40 392 I WBJP SC 3 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Hydropsychidae 7 69 I JP WB BVW CF 6 CG Philopotamidae 28 275 I JP WB BVW CF 2 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Corydalidae 6 59 I JP WB BVW PR 5 CR Coleoptera (beetles) Psephenidae 7 69 I JP WB BVW SC 4 CG Elmidae 8 78 I JP WB BVW SC 4 CG Diptera (true flies) Tipulidae 6 59 I JP WB BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
y
z
Other Total Individuals 102 1001 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-3 STATION NAME Rt 664 Bridge LAT N 39deg 12934rsquo (upstream) LONG W 078deg 05019rsquo (upstream) LAT N 39deg 12951rsquo (downstream) LONG W 078deg 04958rsquo (downstream) FORM COMPILED BY S Robertson W Bousquet RIVER BASIN Potomac River INVESTIGATORS SRobertson BMillholland WBousquet MDahl JPangle
Collection date 28 May 2008 Sheet completion date 11 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Leptophlebiidae 1 15 I SGR BVW CG 2 CR Baetidae 1 15 I BVW CG 5 CG Plecoptera (stoneflies) Perlidae 3 46 I SGR BVW PR 1 CR Nemouridae 1 15 I BVW SH 3 CR Trichoptera (caddisflies) Hydropsychidae 5 77 I SGR BVW CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 27 415 I SGR BVW SC 4 CG Psephenidae 2 31 A SGR BVW SC 4 CG Diptera (true flies) Simuliidae 5 77 I SGR BVW CF 6 CG Chironomidae 20 308 I SGR BVW CG 6 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
aa
bb
Other Total Individuals 65 999 Total Taxa 9
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-3 STATION NAME Rt 664 Bridge LAT N 39deg 12934rsquo (upstream) LONG W 078deg 05019rsquo (upstream) LAT N 39deg 12951rsquo (downstream) LONG W 078deg 04958rsquo (downstream) FORM COMPILED BY S Robertson RIVER BASIN Potomac River INVESTIGATORS S Robertson M Dahl A Fisher H Kepple
Collection date 10 Oct 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Heptageniidae 2 20 I SGR SC 3 CG Isonychiidae 2 20 I BVW CF 2 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Philopotamidae 36 367 I SGR CF 2 CG Hydropsychidae 47 480 I SGR CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 2 20 A SGR SC 4 CG Psephenidae 8 82 A SGR SC 4 CG Diptera (true flies) Tabanidae 1 10 I SGR PR 7 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
cc
dd
Other Total Individuals 98 999 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-4 STATION NAME Unnamed Tributary LAT (upstream) N 39deg 13035rsquo LONG (upstream) W 078deg 05129rsquo LAT (downstream) N 39deg 13000rsquo LONG (downstream) W 078deg 5086rsquo FORM COMPILED BY A Fisher M Dahl rev W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson A Fisher H Kepple
Collection date 31 May 2008 Sheet completion date rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Flatworms (Turbellaria) Worms (Oligochaeta) Naididae 1 14 - BVW CG 8 BU Leeches (Hirudinea) Snails (Gastropoda) Clams (Bivalvia) Isopods (Isopoda) Asellidae 3 42 A AF BVW CG 8 SP Scuds (Amphipoda) Crayfish Shrimp (Decapoda) Water Mites (Hydracarina) Dragonflies (Odonata Anisoptera) Damselflies (Odonata Zygoptera) Mayflies (Ephemeroptera) Stoneflies (Plecoptera) Perlidae 2 28 I AF BVW PR 1 CR Caddisflies (Trichoptera) True Bugs (Hemiptera) Corixidae 1 14 I AF BVW MP 9 DV Dobsonflies (Megaloptera) Beetles (Coleoptera) Dytiscidae 1 14 I AF BVW PR 6 GN True Flies (Diptera) Chironomidae 61 847 I AF BVW CG 6 BU Simuliidae 3 42 I AF BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
ee
ff
Other Total Individuals 72 1001 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-4 STATION NAME Unnamed Tributary LAT (upstream) N 39deg 13035rsquo LONG (upstream) W 078deg 05129rsquo LAT (downstream) N 39deg 13000rsquo LONG (downstream) W 078deg 5086rsquo FORM COMPILED BY Andrew G M Fisher W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson A Fisher H Kepple
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Flatworms (Turbellaria) Worms (Oligochaeta) Lumbriculidae 2 32 A AF BVW CG 8 BU Leeches (Hirudinea) Snails (Gastropoda) Lymnaeidae 1 16 A BVW CG 9 GN Clams (Bivalvia) Isopods (Isopoda) Scuds (Amphipoda) Crayfish Shrimp (Decapoda) Cambaridae 1 16 A AF BVW GN 5 GN Water Mites (Hydracarina) Dragonflies (Odonata Anisoptera) Damselflies (Odonata Zygoptera) Calopterygidae 4 63 I BVW PR 7 CL Mayflies (Ephemeroptera) Tricorythidae 1 16 I BVW CG 5 SP Stoneflies (Plecoptera) Caddisflies (Trichoptera) Phryganeidae 4 63 I AF BVW SH 4 CR Hydropsychidae 6 95 I AF BVW CF 6 CG I True Bugs (Hemiptera) Corixidae 8 127 I AF BVW MP 9 DV (Veliidae ) (7) - I AF BVW ( ) Dobsonflies (Megaloptera) Sialidae 3 48 I AF BVW PR 7 SP Corydalidae 2 32 I AF BVW PR 5 CR Beetles (Coleoptera) Haliplidae 1 16 A AF BVW MP 8 GN Dysticidae 7 111 I AF BVW PR 6 GN Hydrophilidae 1 16 A AF BVW PR 8 GN Elmidae 3 48 I BVW SC 4 CG True Flies (Diptera) Chironomidae 8 127 I AF BVW CG 6 BU Simuliidae 7 111 I AF BVW CF 6 CG Tipulidae 4 63 I AF BVW SH 4 GU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
gg
hh
Other Total Individuals 63
(70) 1000
Total Taxa 17 (18)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Veliidae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-5 STATION NAME Lick Run LAT N 39deg 12752rsquo (upstream) LONG W 078deg 05685rsquo (upstream) LAT N 39deg 12784rsquo (downstream) LONG W 078deg 05631rsquo (downstream) FORM COMPILED BY Brandon Millholland W Bousquet RIVER BASIN Potomac River INVESTIGATORS J Pangle S Robertson W Bousquet JTovar M Dahl
Collection date 29 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Gomphidae 1 15 I BVW PR 3 BU Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Leptophlebiidae 11 167 A LBM BVW CG 2 CR Plecoptera (stoneflies) Perlidae 10 152 A LBM BVW PR 1 CR Perlodiae 1 15 A LBM BVW PR 2 CR Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 5 76 A LBM BVW SC 4 CG Elmidae 2 30 I LBM BVW SC 4 CG Diptera (true flies) (Culicidae) (3) - P LMB BVW ( ) Chironomidae 23 348 I LBM BVW CG 6 BU Simuliidae 13 197 I LBM BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
ii
jj
Other Total Individuals 66
(69) 1000
Total Taxa 7 (8)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Culicidae (Diptera) pupae not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl J Pangle S Robertson W Bousquet B Millholland J Tovar H Kepple
Collection date 30 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 1 12 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Asellidae 114 965 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Plecoptera (stoneflies) Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 2 23 A MRD BVW SC 4 CG Diptera (true flies)
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
kk
ll
Other Total Individuals 117 1000 Total Taxa 3
Notes Subsample taken 29 October 2009 All taxonomic determinations by MRD were confirmed by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson K McCann A Fisher H Kepple A Barrett
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 2 17 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Sphaeriidae 2 17 I MRD BVW CF 8 BU Isopoda (isopods) Asellidae 99 853 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Calopterygidae 2 17 A MRD BVW PR 7 CL Ephemeroptera (mayflies) Leptophlebiidae 2 17 I MRD BVW CG 2 CR Heptageniidae 4 34 A MRD BVW SC 3 CG Plecoptera (stoneflies) Perlidae 1 09 A MRD BVW PR 1 CR Trichoptera (caddisflies) Hemiptera (true bugs) (Gerridae) (1) - A MRD BVW ( ) Corixidae 1 09 A MRD BVW PR 5 CR Megaloptera (dobsonflies etc) Coleoptera (beetles) Dryopidae 1 09 A MRD BVW SC 5 CG Diptera (true flies) Tipulidae 2 17 I BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
mm
nn
Other Total Individuals 116
(117) 999
Total Taxa 10 (11)
Notes Subsample taken 6 November 2008 Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009 Gerridae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
- Disclaimer ii
- Title Page iii
- Acknowledgements v
- Table of Contents vii
- SECTION 2 RESULTS DATA TABLES 7
- OVERVIEW AND FINDINGS
- Introduction
- RESULTS DATA TABLES
- REFERENCES
- FIELD DATA SHEETS
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- SubstrateType
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- WATER QUALITY ANALYSIS
- THROUGH THE VIRGINIA
- STREAM CONDITION INDEX (VSCI)
- LABORATORY BENCH SHEETS
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION Lick Run STATION HLR-5 STREAM CLASS LAT N 39 127517 (upstream) LONG W 078 05685(upstream) LAT N 39 12784 (downstream) LONG W 078 05631 (downstream) FORM COMPILED BY B Millholland RIVER BASIN Potomac River INVESTIGATORS B Millholland JTovar SRobertson MDahl WBousquet JPangle
DATE 29 May 2008 TIME 1040 AM (start)
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project
k
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) x YES NO
rain (steady rain) Air Temperature __15___degC showers (intermittent) Comments _______________________
_5_ X cloud cover _0__ ______________________________
x clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane Significant springs andor seeps x Valley Marsh or swamp Montane amp Valley Mixture of origins Other ___________________
COMMENTS Lick Run appears to be intermittent and may be dry in Fall 2008
l
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence x FieldPasture Industrial Obvious sources Agricultural Other ____________ Adjacent logging stream bank scour Residential Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left 85 young deciduous forest 15 logged Bank=Soil vegetation and cobbles Right Young deciduous forest on 30 slope Bank= Soil vegetation and cobble Indicate the dominant vegetation type(s) and record the dominant species
x Trees Shrubs Grasses Forbs Dominant species Mixed Deciduous INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width __11__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area 110 m2
Estimated stream depth _009__m Proportion of Reach Represented by Stream High water mark __96___m Morphology Types Surface velocity (at thalweg) __01___msec x Riffle__30___ x Run__5___ x Pool__65___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___0____ m2 Density of LWD ___-____ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __70___ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present__________________________________________________________
WATER QUALITY Temperature __14___degC Water Odors x NormalNone Sewage Specific Conductance __-__ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ___-___ Water Surface Oils Slick Sheen Globs Flecks pH __-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used _____NA______ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other_____None_____
SEDIMENT AND SUBSTRATE
Odors Deposits Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other________________ x Other Slightly Salty Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 0 Detritus sticks wood coarse plant materials
5 Boulder gt256 mm (10rsquorsquo) 0
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 75 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 23 Sand 006-2 mm (gritty) 2 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION McCann Farm STATION HLR-6 STREAM CLASS LAT N 39 13076 (upstream) LONG W 078 06539 (upstream) LAT N 39 13063 (downstream) LONG W 078 06473 (downstream) FORM COMPILED BY MDahl RIVER BASIN Potomac River INVESTIGATORS MDahl JPangle SRobertson W Bousqet BMillholland JTovar H Kepple
DATE 30 May 2008 TIME 1050 AM (start)
PROJECT NAME
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) YES x NO
rain (steady rain) Air Temperature __18___degC showers (intermittent) Comments _______________________
_85_x cloud cover _10__ ______________________________
clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x (Significant springs andor seeps) x ( Valley) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS Multi-habitat sampling with D-frame nets and seine
m
n
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial x No evidence Some evidence x FieldPasture Industrial Obvious sources x Agricultural Other ____________ Minor stream bank scour Residential Local Watershed Erosion x None Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left 35 bank grasses scattered trees and some bushes Right High bank with grasses and scattered trees Indicate the dominant vegetation type(s) and record the dominant species
x Trees Shrubs Grasses Forbs Dominant species Black Walnut and Festuca elatior
INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width __2___m Fully open Partly open x Partly shaded Fully shaded Sampling reach area __200_m2
Estimated stream depth __03_m Proportion of Reach Represented by Stream High water mark __1___m Morphology Types Surface velocity (at thalweg) __166_msec x Riffle__0___ x Run__55___ x Pool__45___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___2____ m2 Density of LWD ___-____ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __20_____ x Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present ____Glyceria (grass)sp__________
WATER QUALITY Temperature __16___degC Water Odors x NormalNone Sewage Specific Conductance ___-___ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ____-____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used ______NA___ Clear x Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other__NONE__ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 0 Detritus sticks wood coarse plant materials
5 Boulder gt256 mm (10rsquorsquo) 0
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 27 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 38 Sand 006-2 mm (gritty) 5 Marl grey shell
fragments 0 (lt1)
Silt 0004-006 mm 25 Clay lt 0004 mm (slick) 5 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Appendix B
WATER QUALITY ANALYSIS THROUGH THE VIRGINIA
STREAM CONDITION INDEX (VSCI)
The Virginia Stream Condition Index (VSCI) relies on eight metrics to categorize stream health According to Burton and Gerritsen (2003 p 1-1) this index ldquohellip can be used as a primary indicator of ecosystem health and can identify impairment with respect to the reference (or natural) conditionrdquo These metrics are numeric measures of a biological communityrsquos species composition diversity ecological niches or tolerance to pollution
Table 13 on the next page describes each VSCI metric lists its formula and provides the best standard value (BSV) for that metric Based on a 100-point scale the BSV is the score that reflects optimum conditions for aquatic life ie the best water quality value possible (DEQ 2006b pp 8 33) The table also explains whether the score increases or decreases with environmental stress
As Table 13 indicates each of the eight VSCI metrics is calculated individually Each formula provides a unitless score based on a 100-point scale with 0 as the worst possible score and 100 as the best Any metric that provides a result above 100 is changed to 100 in order for the index to work properly After the eight metrics are calculated they are summed and averaged to provide a final VSCI score on a 100-point scale
Based on statistical analysis of Virginiarsquos streams and their BMI communities the DEQ (2006b p 8) currently uses the following tiers to interpret VSCI scores
VSCI Scores Aquatic Life Use
(ALI) Tiers
lt 42 severely stressed
42-55 moderately stressed
55-63 unknownfair (gray zone)
63-73 good
gt73 excellent
Section 2 of this report presents the results of calculations for the six BMI sampling sites the research team studied in the Hiatt-Lick Run subwatershed in 2008
o
p
1 Total taxa the number of distinct taxa in the sample measures the overall diversity of the macroinvertebrate assemblage The best standard value (BSV) is 189 Score = 100 x (X189) Total taxa decreases with stress 2 EPT taxa the sum of the distinct taxa in the orders Ephemeroptera (mayflies) Plecoptera (stoneflies) and Trichoptera (caddisflies) These orders are known to be sensitive to pollution The best standard value (BSV) is 117 Score = 100 x (X117) EPT taxa decreases with stress 3 Percent Ephemeroptera the percent abundance of individuals in the sample that belong to the order Ephemeroptera (mayflies) The best standard value (BSV) is 477 Score = 100 x (X477) Percent Ephemeroptera decreases with stress 4 Percent Plecoptera + Trichoptera less Hydropsychidae the percent abundance of individuals in the sample that belong to the orders Plecoptera (stoneflies) and Trichoptera (caddisflies) excluding the pollution-tolerant caddisfly family Hydropsychidae The best standard value (BSV) is 562 Score = 100 x (X562) Percent Plecoptera + Trichoptera less Hydropsychidae decreases with stress 5 Percent Scrapers the percent abundance of individuals in the sample whose primary functional mechanism for feeding is grazing on the substrate or periphyton (ie attached algae and associated material) The best standard value (BSV) is 467 Score = 100 x (X467) Percent scrapers decreases with stress 6 Percent Chironomidae the percent abundance of individuals in the sample that are Chironomidae (non-biting midge) larvae and pupae The best standard value (BSV) is 16 Score = 100 x [(100-X)(100-16)] Percent Chironomidae increases with stress 7 Percent Top Two Dominant the percent abundance of the two most frequent taxa present in the sample The best standard value (BSV) is 328 Score = 100 x [(100-X)(100-328)] The Percent Top 2 Dominant increases with stress 8 Hilsenhoff Biotic Index (HBI) an abundance-weighted average pollution tolerance of all the individuals within a sample keyed to the family level Tolerance ratings range from 1 (highly intolerant) to 10 (highly tolerant) The best standard value (BSV) is 29 Score = 100 x [(10-X)(10-29)] The Hilsenhoff Biotic Index (HBI) increases with stress ______________________________________________________________________________ Table 13 Description of Benthic Macroinvertebrate VSCI Metrics Includes responses (increases decreases) of each VSCI metric to degraded water quality conditions Adapted from Burton and Gerritsen (2003 pp 3-15 3-16 6-2) and DEQ (2008)
q
Appendix C
LABORATORY BENCH SHEETS
Shenandoah Universityrsquos Environmental Studies Department developed the Benthic Macroinvertebrate Laboratory Bench Sheet and VSCI Worksheet to record BMI specimen identifications and their VSCI classifications for each sample
This form is based in part on bench sheets developed by the EPA and Virginia DEQ for recording specimen identifications Additional columns on Shenandoah Universityrsquos forms for relative numbers and VSCI classifications make the calculation and verification of VSCI scores easier
Note the column for taxonomistrsquos initials Specimens were identified by students in Shenandoah Universityrsquos research group and then verified or where necessary changed by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality This external review helps assure the validity of the study Originals of these bench sheets with handwritten taxonomistsrsquo initials will be kept on file at Shenandoah Universityrsquos Environmental Studies Department for five years after the completion of this study
r
s
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-1 STATION NAME Mouth LAT N 39deg 12749rsquo (upstream) LONG W 078deg 04209rsquo (upstream) LAT N 39deg 12729rsquo (downstream) LONG W 078deg 04148rsquo (downstream) FORM COMPILED BY Jeremy Tovar W S Bousquet RIVER BASIN Potomac River INVESTIGATORS Marie Dahl Brandon Millholand J W Pangle Sean Robertson Jeremy Tovar Woodward Bousquet Ben Bousquet
Collection date 21 May 2008 Sheet completion date 12 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Hirudinea 3 33 JT BVW PR 8 SP Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Coenagrionidae 2 22 BVW PR 9 CR Ephemeroptera (mayflies) Caenidae 1 11 JT BVW CG 6 SP Ephemerllidae 4 44 JT BVW CG 2 CR Heptageniidae 5 55 JT BVW SC 3 CG Isonychiidae 1 11 BVW CF 3 CG Plecoptera (stoneflies) Perlidae 14 154 JT BVW PR 2 CR Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Corydalidae 1 11 JT BVW PR 5 CR Coleoptera (beetles) Elmidae 24 264 I JT BVW SC 4 CG Dytiscidae 4 44 JT BVW PR 6 GN Psephenidae 3 33 JT BVW SC 4 CG Diptera (true flies) Tipulidae 3 33 JT BVW SH 4 BU Chironomidae 26 286 JT BVW CG 6 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
t
Other Total Individuals 91 1001 Total Taxa 13
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
u
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-1 STATION NAME Mouth LAT N 39deg 12749rsquo (upstream) LONG W 078deg 04209rsquo (upstream) LAT N 39deg 12729rsquo (downstream) LONG W 078deg 04148rsquo (downstream) FORM COMPILED BY Jeremy Tovar W S Bousquet RIVER BASIN Potomac River INVESTIGATORS Brandon Millholand JW Pangle Jeremy Tovar Heather Kepple
Collection date 10 October 2008 Sheet completion date 12 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Oligochaeta 3 35 JT BVW CG 8 BU Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Coenagrionidae 2 23 JT BVW PR 9 CR Ephemeroptera (mayflies) Heptageniidae 12 140 JT BVW SC 3 CG Isonychiidae 2 23 BVW CF 3 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Hydropsychidae 16 186 JT BVW CF 6 CG Philopotamidae 31 360 JT BVW CF 2 CG Hemiptera (true bugs) (Veliidae) (5) - JT BVW ( ) Megaloptera (dobsonflies etc) Corydalidae 10 116 JT BVW PR 5 CR Coleoptera (beetles) Dytiscidae 1 12 JT BVW PR 6 GN Psephenidae 7 81 JT BVW SC 4 CG Diptera (true flies) Tipulidae 2 23 BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
v
Other Total Individuals 86
(91) 999
Total Taxa 10 (11)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009 Veliidae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
w
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-2 STATION NAME Package Plant LAT N 39deg 12958rsquo (upstream) LONG W 078deg 04900 (upstream) LAT N 39deg 12958rsquo (downstream) LONG W 078deg 04822 (downstream) FORM COMPILED BY JW Pangle W Bousquet RIVER BASIN Potomac River INVESTIGATORS JW Pangle S Robertson J Tovar M Dahl B Millholland W Bousquet
Collection date 29 May 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Plecoptera (stoneflies) Perlidae 11 136 I JP BVW PR 1 CR Trichoptera (caddisflies) Hydropsychidae 10 123 I JP BVW CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 14 173 I JP BVW SC 4 CG Diptera (true flies) Chironomidae 32 395 I JP BVW CG 6 BU Simuliidae 14 173 I JP BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
x
Other Total Individuals 81 1000 Total Taxa 5
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-2 STATION NAME Package Plant LAT N 39deg 12958rsquo (upstream) LONG W 078deg 04900 (upstream) LAT N 39deg 12958rsquo (downstream) LONG W 078deg 04822 (downstream) FORM COMPILED BY JW Pangle W Bousquet RIVER BASIN Potomac River INVESTIGATORS JW Pangle Brandon Millholland Jeremy Tovar Heather Kepple
Collection date 10 October 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Heptageniidae 40 392 I WBJP SC 3 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Hydropsychidae 7 69 I JP WB BVW CF 6 CG Philopotamidae 28 275 I JP WB BVW CF 2 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Corydalidae 6 59 I JP WB BVW PR 5 CR Coleoptera (beetles) Psephenidae 7 69 I JP WB BVW SC 4 CG Elmidae 8 78 I JP WB BVW SC 4 CG Diptera (true flies) Tipulidae 6 59 I JP WB BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
y
z
Other Total Individuals 102 1001 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-3 STATION NAME Rt 664 Bridge LAT N 39deg 12934rsquo (upstream) LONG W 078deg 05019rsquo (upstream) LAT N 39deg 12951rsquo (downstream) LONG W 078deg 04958rsquo (downstream) FORM COMPILED BY S Robertson W Bousquet RIVER BASIN Potomac River INVESTIGATORS SRobertson BMillholland WBousquet MDahl JPangle
Collection date 28 May 2008 Sheet completion date 11 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Leptophlebiidae 1 15 I SGR BVW CG 2 CR Baetidae 1 15 I BVW CG 5 CG Plecoptera (stoneflies) Perlidae 3 46 I SGR BVW PR 1 CR Nemouridae 1 15 I BVW SH 3 CR Trichoptera (caddisflies) Hydropsychidae 5 77 I SGR BVW CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 27 415 I SGR BVW SC 4 CG Psephenidae 2 31 A SGR BVW SC 4 CG Diptera (true flies) Simuliidae 5 77 I SGR BVW CF 6 CG Chironomidae 20 308 I SGR BVW CG 6 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
aa
bb
Other Total Individuals 65 999 Total Taxa 9
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-3 STATION NAME Rt 664 Bridge LAT N 39deg 12934rsquo (upstream) LONG W 078deg 05019rsquo (upstream) LAT N 39deg 12951rsquo (downstream) LONG W 078deg 04958rsquo (downstream) FORM COMPILED BY S Robertson RIVER BASIN Potomac River INVESTIGATORS S Robertson M Dahl A Fisher H Kepple
Collection date 10 Oct 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Heptageniidae 2 20 I SGR SC 3 CG Isonychiidae 2 20 I BVW CF 2 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Philopotamidae 36 367 I SGR CF 2 CG Hydropsychidae 47 480 I SGR CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 2 20 A SGR SC 4 CG Psephenidae 8 82 A SGR SC 4 CG Diptera (true flies) Tabanidae 1 10 I SGR PR 7 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
cc
dd
Other Total Individuals 98 999 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-4 STATION NAME Unnamed Tributary LAT (upstream) N 39deg 13035rsquo LONG (upstream) W 078deg 05129rsquo LAT (downstream) N 39deg 13000rsquo LONG (downstream) W 078deg 5086rsquo FORM COMPILED BY A Fisher M Dahl rev W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson A Fisher H Kepple
Collection date 31 May 2008 Sheet completion date rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Flatworms (Turbellaria) Worms (Oligochaeta) Naididae 1 14 - BVW CG 8 BU Leeches (Hirudinea) Snails (Gastropoda) Clams (Bivalvia) Isopods (Isopoda) Asellidae 3 42 A AF BVW CG 8 SP Scuds (Amphipoda) Crayfish Shrimp (Decapoda) Water Mites (Hydracarina) Dragonflies (Odonata Anisoptera) Damselflies (Odonata Zygoptera) Mayflies (Ephemeroptera) Stoneflies (Plecoptera) Perlidae 2 28 I AF BVW PR 1 CR Caddisflies (Trichoptera) True Bugs (Hemiptera) Corixidae 1 14 I AF BVW MP 9 DV Dobsonflies (Megaloptera) Beetles (Coleoptera) Dytiscidae 1 14 I AF BVW PR 6 GN True Flies (Diptera) Chironomidae 61 847 I AF BVW CG 6 BU Simuliidae 3 42 I AF BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
ee
ff
Other Total Individuals 72 1001 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-4 STATION NAME Unnamed Tributary LAT (upstream) N 39deg 13035rsquo LONG (upstream) W 078deg 05129rsquo LAT (downstream) N 39deg 13000rsquo LONG (downstream) W 078deg 5086rsquo FORM COMPILED BY Andrew G M Fisher W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson A Fisher H Kepple
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Flatworms (Turbellaria) Worms (Oligochaeta) Lumbriculidae 2 32 A AF BVW CG 8 BU Leeches (Hirudinea) Snails (Gastropoda) Lymnaeidae 1 16 A BVW CG 9 GN Clams (Bivalvia) Isopods (Isopoda) Scuds (Amphipoda) Crayfish Shrimp (Decapoda) Cambaridae 1 16 A AF BVW GN 5 GN Water Mites (Hydracarina) Dragonflies (Odonata Anisoptera) Damselflies (Odonata Zygoptera) Calopterygidae 4 63 I BVW PR 7 CL Mayflies (Ephemeroptera) Tricorythidae 1 16 I BVW CG 5 SP Stoneflies (Plecoptera) Caddisflies (Trichoptera) Phryganeidae 4 63 I AF BVW SH 4 CR Hydropsychidae 6 95 I AF BVW CF 6 CG I True Bugs (Hemiptera) Corixidae 8 127 I AF BVW MP 9 DV (Veliidae ) (7) - I AF BVW ( ) Dobsonflies (Megaloptera) Sialidae 3 48 I AF BVW PR 7 SP Corydalidae 2 32 I AF BVW PR 5 CR Beetles (Coleoptera) Haliplidae 1 16 A AF BVW MP 8 GN Dysticidae 7 111 I AF BVW PR 6 GN Hydrophilidae 1 16 A AF BVW PR 8 GN Elmidae 3 48 I BVW SC 4 CG True Flies (Diptera) Chironomidae 8 127 I AF BVW CG 6 BU Simuliidae 7 111 I AF BVW CF 6 CG Tipulidae 4 63 I AF BVW SH 4 GU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
gg
hh
Other Total Individuals 63
(70) 1000
Total Taxa 17 (18)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Veliidae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-5 STATION NAME Lick Run LAT N 39deg 12752rsquo (upstream) LONG W 078deg 05685rsquo (upstream) LAT N 39deg 12784rsquo (downstream) LONG W 078deg 05631rsquo (downstream) FORM COMPILED BY Brandon Millholland W Bousquet RIVER BASIN Potomac River INVESTIGATORS J Pangle S Robertson W Bousquet JTovar M Dahl
Collection date 29 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Gomphidae 1 15 I BVW PR 3 BU Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Leptophlebiidae 11 167 A LBM BVW CG 2 CR Plecoptera (stoneflies) Perlidae 10 152 A LBM BVW PR 1 CR Perlodiae 1 15 A LBM BVW PR 2 CR Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 5 76 A LBM BVW SC 4 CG Elmidae 2 30 I LBM BVW SC 4 CG Diptera (true flies) (Culicidae) (3) - P LMB BVW ( ) Chironomidae 23 348 I LBM BVW CG 6 BU Simuliidae 13 197 I LBM BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
ii
jj
Other Total Individuals 66
(69) 1000
Total Taxa 7 (8)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Culicidae (Diptera) pupae not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl J Pangle S Robertson W Bousquet B Millholland J Tovar H Kepple
Collection date 30 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 1 12 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Asellidae 114 965 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Plecoptera (stoneflies) Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 2 23 A MRD BVW SC 4 CG Diptera (true flies)
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
kk
ll
Other Total Individuals 117 1000 Total Taxa 3
Notes Subsample taken 29 October 2009 All taxonomic determinations by MRD were confirmed by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson K McCann A Fisher H Kepple A Barrett
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 2 17 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Sphaeriidae 2 17 I MRD BVW CF 8 BU Isopoda (isopods) Asellidae 99 853 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Calopterygidae 2 17 A MRD BVW PR 7 CL Ephemeroptera (mayflies) Leptophlebiidae 2 17 I MRD BVW CG 2 CR Heptageniidae 4 34 A MRD BVW SC 3 CG Plecoptera (stoneflies) Perlidae 1 09 A MRD BVW PR 1 CR Trichoptera (caddisflies) Hemiptera (true bugs) (Gerridae) (1) - A MRD BVW ( ) Corixidae 1 09 A MRD BVW PR 5 CR Megaloptera (dobsonflies etc) Coleoptera (beetles) Dryopidae 1 09 A MRD BVW SC 5 CG Diptera (true flies) Tipulidae 2 17 I BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
mm
nn
Other Total Individuals 116
(117) 999
Total Taxa 10 (11)
Notes Subsample taken 6 November 2008 Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009 Gerridae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
- Disclaimer ii
- Title Page iii
- Acknowledgements v
- Table of Contents vii
- SECTION 2 RESULTS DATA TABLES 7
- OVERVIEW AND FINDINGS
- Introduction
- RESULTS DATA TABLES
- REFERENCES
- FIELD DATA SHEETS
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- SubstrateType
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- WATER QUALITY ANALYSIS
- THROUGH THE VIRGINIA
- STREAM CONDITION INDEX (VSCI)
- LABORATORY BENCH SHEETS
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
l
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial No evidence x Some evidence x FieldPasture Industrial Obvious sources Agricultural Other ____________ Adjacent logging stream bank scour Residential Local Watershed Erosion None x Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left 85 young deciduous forest 15 logged Bank=Soil vegetation and cobbles Right Young deciduous forest on 30 slope Bank= Soil vegetation and cobble Indicate the dominant vegetation type(s) and record the dominant species
x Trees Shrubs Grasses Forbs Dominant species Mixed Deciduous INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width __11__m Fully open x Partly open Partly shaded Fully shaded Sampling reach area 110 m2
Estimated stream depth _009__m Proportion of Reach Represented by Stream High water mark __96___m Morphology Types Surface velocity (at thalweg) __01___msec x Riffle__30___ x Run__5___ x Pool__65___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___0____ m2 Density of LWD ___-____ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __70___ Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present__________________________________________________________
WATER QUALITY Temperature __14___degC Water Odors x NormalNone Sewage Specific Conductance __-__ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ___-___ Water Surface Oils Slick Sheen Globs Flecks pH __-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used _____NA______ x Clear Slightly Turbid Turbid ________________________________ Opaque Stained Other_____None_____
SEDIMENT AND SUBSTRATE
Odors Deposits Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other________________ x Other Slightly Salty Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 0 Detritus sticks wood coarse plant materials
5 Boulder gt256 mm (10rsquorsquo) 0
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 75 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 23 Sand 006-2 mm (gritty) 2 Marl grey shell
fragments 0
Silt 0004-006 mm 0 Clay lt 0004 mm (slick) 0 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION McCann Farm STATION HLR-6 STREAM CLASS LAT N 39 13076 (upstream) LONG W 078 06539 (upstream) LAT N 39 13063 (downstream) LONG W 078 06473 (downstream) FORM COMPILED BY MDahl RIVER BASIN Potomac River INVESTIGATORS MDahl JPangle SRobertson W Bousqet BMillholland JTovar H Kepple
DATE 30 May 2008 TIME 1050 AM (start)
PROJECT NAME
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) YES x NO
rain (steady rain) Air Temperature __18___degC showers (intermittent) Comments _______________________
_85_x cloud cover _10__ ______________________________
clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x (Significant springs andor seeps) x ( Valley) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS Multi-habitat sampling with D-frame nets and seine
m
n
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial x No evidence Some evidence x FieldPasture Industrial Obvious sources x Agricultural Other ____________ Minor stream bank scour Residential Local Watershed Erosion x None Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left 35 bank grasses scattered trees and some bushes Right High bank with grasses and scattered trees Indicate the dominant vegetation type(s) and record the dominant species
x Trees Shrubs Grasses Forbs Dominant species Black Walnut and Festuca elatior
INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width __2___m Fully open Partly open x Partly shaded Fully shaded Sampling reach area __200_m2
Estimated stream depth __03_m Proportion of Reach Represented by Stream High water mark __1___m Morphology Types Surface velocity (at thalweg) __166_msec x Riffle__0___ x Run__55___ x Pool__45___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___2____ m2 Density of LWD ___-____ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __20_____ x Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present ____Glyceria (grass)sp__________
WATER QUALITY Temperature __16___degC Water Odors x NormalNone Sewage Specific Conductance ___-___ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ____-____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used ______NA___ Clear x Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other__NONE__ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 0 Detritus sticks wood coarse plant materials
5 Boulder gt256 mm (10rsquorsquo) 0
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 27 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 38 Sand 006-2 mm (gritty) 5 Marl grey shell
fragments 0 (lt1)
Silt 0004-006 mm 25 Clay lt 0004 mm (slick) 5 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Appendix B
WATER QUALITY ANALYSIS THROUGH THE VIRGINIA
STREAM CONDITION INDEX (VSCI)
The Virginia Stream Condition Index (VSCI) relies on eight metrics to categorize stream health According to Burton and Gerritsen (2003 p 1-1) this index ldquohellip can be used as a primary indicator of ecosystem health and can identify impairment with respect to the reference (or natural) conditionrdquo These metrics are numeric measures of a biological communityrsquos species composition diversity ecological niches or tolerance to pollution
Table 13 on the next page describes each VSCI metric lists its formula and provides the best standard value (BSV) for that metric Based on a 100-point scale the BSV is the score that reflects optimum conditions for aquatic life ie the best water quality value possible (DEQ 2006b pp 8 33) The table also explains whether the score increases or decreases with environmental stress
As Table 13 indicates each of the eight VSCI metrics is calculated individually Each formula provides a unitless score based on a 100-point scale with 0 as the worst possible score and 100 as the best Any metric that provides a result above 100 is changed to 100 in order for the index to work properly After the eight metrics are calculated they are summed and averaged to provide a final VSCI score on a 100-point scale
Based on statistical analysis of Virginiarsquos streams and their BMI communities the DEQ (2006b p 8) currently uses the following tiers to interpret VSCI scores
VSCI Scores Aquatic Life Use
(ALI) Tiers
lt 42 severely stressed
42-55 moderately stressed
55-63 unknownfair (gray zone)
63-73 good
gt73 excellent
Section 2 of this report presents the results of calculations for the six BMI sampling sites the research team studied in the Hiatt-Lick Run subwatershed in 2008
o
p
1 Total taxa the number of distinct taxa in the sample measures the overall diversity of the macroinvertebrate assemblage The best standard value (BSV) is 189 Score = 100 x (X189) Total taxa decreases with stress 2 EPT taxa the sum of the distinct taxa in the orders Ephemeroptera (mayflies) Plecoptera (stoneflies) and Trichoptera (caddisflies) These orders are known to be sensitive to pollution The best standard value (BSV) is 117 Score = 100 x (X117) EPT taxa decreases with stress 3 Percent Ephemeroptera the percent abundance of individuals in the sample that belong to the order Ephemeroptera (mayflies) The best standard value (BSV) is 477 Score = 100 x (X477) Percent Ephemeroptera decreases with stress 4 Percent Plecoptera + Trichoptera less Hydropsychidae the percent abundance of individuals in the sample that belong to the orders Plecoptera (stoneflies) and Trichoptera (caddisflies) excluding the pollution-tolerant caddisfly family Hydropsychidae The best standard value (BSV) is 562 Score = 100 x (X562) Percent Plecoptera + Trichoptera less Hydropsychidae decreases with stress 5 Percent Scrapers the percent abundance of individuals in the sample whose primary functional mechanism for feeding is grazing on the substrate or periphyton (ie attached algae and associated material) The best standard value (BSV) is 467 Score = 100 x (X467) Percent scrapers decreases with stress 6 Percent Chironomidae the percent abundance of individuals in the sample that are Chironomidae (non-biting midge) larvae and pupae The best standard value (BSV) is 16 Score = 100 x [(100-X)(100-16)] Percent Chironomidae increases with stress 7 Percent Top Two Dominant the percent abundance of the two most frequent taxa present in the sample The best standard value (BSV) is 328 Score = 100 x [(100-X)(100-328)] The Percent Top 2 Dominant increases with stress 8 Hilsenhoff Biotic Index (HBI) an abundance-weighted average pollution tolerance of all the individuals within a sample keyed to the family level Tolerance ratings range from 1 (highly intolerant) to 10 (highly tolerant) The best standard value (BSV) is 29 Score = 100 x [(10-X)(10-29)] The Hilsenhoff Biotic Index (HBI) increases with stress ______________________________________________________________________________ Table 13 Description of Benthic Macroinvertebrate VSCI Metrics Includes responses (increases decreases) of each VSCI metric to degraded water quality conditions Adapted from Burton and Gerritsen (2003 pp 3-15 3-16 6-2) and DEQ (2008)
q
Appendix C
LABORATORY BENCH SHEETS
Shenandoah Universityrsquos Environmental Studies Department developed the Benthic Macroinvertebrate Laboratory Bench Sheet and VSCI Worksheet to record BMI specimen identifications and their VSCI classifications for each sample
This form is based in part on bench sheets developed by the EPA and Virginia DEQ for recording specimen identifications Additional columns on Shenandoah Universityrsquos forms for relative numbers and VSCI classifications make the calculation and verification of VSCI scores easier
Note the column for taxonomistrsquos initials Specimens were identified by students in Shenandoah Universityrsquos research group and then verified or where necessary changed by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality This external review helps assure the validity of the study Originals of these bench sheets with handwritten taxonomistsrsquo initials will be kept on file at Shenandoah Universityrsquos Environmental Studies Department for five years after the completion of this study
r
s
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-1 STATION NAME Mouth LAT N 39deg 12749rsquo (upstream) LONG W 078deg 04209rsquo (upstream) LAT N 39deg 12729rsquo (downstream) LONG W 078deg 04148rsquo (downstream) FORM COMPILED BY Jeremy Tovar W S Bousquet RIVER BASIN Potomac River INVESTIGATORS Marie Dahl Brandon Millholand J W Pangle Sean Robertson Jeremy Tovar Woodward Bousquet Ben Bousquet
Collection date 21 May 2008 Sheet completion date 12 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Hirudinea 3 33 JT BVW PR 8 SP Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Coenagrionidae 2 22 BVW PR 9 CR Ephemeroptera (mayflies) Caenidae 1 11 JT BVW CG 6 SP Ephemerllidae 4 44 JT BVW CG 2 CR Heptageniidae 5 55 JT BVW SC 3 CG Isonychiidae 1 11 BVW CF 3 CG Plecoptera (stoneflies) Perlidae 14 154 JT BVW PR 2 CR Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Corydalidae 1 11 JT BVW PR 5 CR Coleoptera (beetles) Elmidae 24 264 I JT BVW SC 4 CG Dytiscidae 4 44 JT BVW PR 6 GN Psephenidae 3 33 JT BVW SC 4 CG Diptera (true flies) Tipulidae 3 33 JT BVW SH 4 BU Chironomidae 26 286 JT BVW CG 6 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
t
Other Total Individuals 91 1001 Total Taxa 13
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
u
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-1 STATION NAME Mouth LAT N 39deg 12749rsquo (upstream) LONG W 078deg 04209rsquo (upstream) LAT N 39deg 12729rsquo (downstream) LONG W 078deg 04148rsquo (downstream) FORM COMPILED BY Jeremy Tovar W S Bousquet RIVER BASIN Potomac River INVESTIGATORS Brandon Millholand JW Pangle Jeremy Tovar Heather Kepple
Collection date 10 October 2008 Sheet completion date 12 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Oligochaeta 3 35 JT BVW CG 8 BU Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Coenagrionidae 2 23 JT BVW PR 9 CR Ephemeroptera (mayflies) Heptageniidae 12 140 JT BVW SC 3 CG Isonychiidae 2 23 BVW CF 3 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Hydropsychidae 16 186 JT BVW CF 6 CG Philopotamidae 31 360 JT BVW CF 2 CG Hemiptera (true bugs) (Veliidae) (5) - JT BVW ( ) Megaloptera (dobsonflies etc) Corydalidae 10 116 JT BVW PR 5 CR Coleoptera (beetles) Dytiscidae 1 12 JT BVW PR 6 GN Psephenidae 7 81 JT BVW SC 4 CG Diptera (true flies) Tipulidae 2 23 BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
v
Other Total Individuals 86
(91) 999
Total Taxa 10 (11)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009 Veliidae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
w
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-2 STATION NAME Package Plant LAT N 39deg 12958rsquo (upstream) LONG W 078deg 04900 (upstream) LAT N 39deg 12958rsquo (downstream) LONG W 078deg 04822 (downstream) FORM COMPILED BY JW Pangle W Bousquet RIVER BASIN Potomac River INVESTIGATORS JW Pangle S Robertson J Tovar M Dahl B Millholland W Bousquet
Collection date 29 May 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Plecoptera (stoneflies) Perlidae 11 136 I JP BVW PR 1 CR Trichoptera (caddisflies) Hydropsychidae 10 123 I JP BVW CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 14 173 I JP BVW SC 4 CG Diptera (true flies) Chironomidae 32 395 I JP BVW CG 6 BU Simuliidae 14 173 I JP BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
x
Other Total Individuals 81 1000 Total Taxa 5
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-2 STATION NAME Package Plant LAT N 39deg 12958rsquo (upstream) LONG W 078deg 04900 (upstream) LAT N 39deg 12958rsquo (downstream) LONG W 078deg 04822 (downstream) FORM COMPILED BY JW Pangle W Bousquet RIVER BASIN Potomac River INVESTIGATORS JW Pangle Brandon Millholland Jeremy Tovar Heather Kepple
Collection date 10 October 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Heptageniidae 40 392 I WBJP SC 3 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Hydropsychidae 7 69 I JP WB BVW CF 6 CG Philopotamidae 28 275 I JP WB BVW CF 2 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Corydalidae 6 59 I JP WB BVW PR 5 CR Coleoptera (beetles) Psephenidae 7 69 I JP WB BVW SC 4 CG Elmidae 8 78 I JP WB BVW SC 4 CG Diptera (true flies) Tipulidae 6 59 I JP WB BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
y
z
Other Total Individuals 102 1001 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-3 STATION NAME Rt 664 Bridge LAT N 39deg 12934rsquo (upstream) LONG W 078deg 05019rsquo (upstream) LAT N 39deg 12951rsquo (downstream) LONG W 078deg 04958rsquo (downstream) FORM COMPILED BY S Robertson W Bousquet RIVER BASIN Potomac River INVESTIGATORS SRobertson BMillholland WBousquet MDahl JPangle
Collection date 28 May 2008 Sheet completion date 11 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Leptophlebiidae 1 15 I SGR BVW CG 2 CR Baetidae 1 15 I BVW CG 5 CG Plecoptera (stoneflies) Perlidae 3 46 I SGR BVW PR 1 CR Nemouridae 1 15 I BVW SH 3 CR Trichoptera (caddisflies) Hydropsychidae 5 77 I SGR BVW CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 27 415 I SGR BVW SC 4 CG Psephenidae 2 31 A SGR BVW SC 4 CG Diptera (true flies) Simuliidae 5 77 I SGR BVW CF 6 CG Chironomidae 20 308 I SGR BVW CG 6 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
aa
bb
Other Total Individuals 65 999 Total Taxa 9
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-3 STATION NAME Rt 664 Bridge LAT N 39deg 12934rsquo (upstream) LONG W 078deg 05019rsquo (upstream) LAT N 39deg 12951rsquo (downstream) LONG W 078deg 04958rsquo (downstream) FORM COMPILED BY S Robertson RIVER BASIN Potomac River INVESTIGATORS S Robertson M Dahl A Fisher H Kepple
Collection date 10 Oct 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Heptageniidae 2 20 I SGR SC 3 CG Isonychiidae 2 20 I BVW CF 2 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Philopotamidae 36 367 I SGR CF 2 CG Hydropsychidae 47 480 I SGR CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 2 20 A SGR SC 4 CG Psephenidae 8 82 A SGR SC 4 CG Diptera (true flies) Tabanidae 1 10 I SGR PR 7 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
cc
dd
Other Total Individuals 98 999 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-4 STATION NAME Unnamed Tributary LAT (upstream) N 39deg 13035rsquo LONG (upstream) W 078deg 05129rsquo LAT (downstream) N 39deg 13000rsquo LONG (downstream) W 078deg 5086rsquo FORM COMPILED BY A Fisher M Dahl rev W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson A Fisher H Kepple
Collection date 31 May 2008 Sheet completion date rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Flatworms (Turbellaria) Worms (Oligochaeta) Naididae 1 14 - BVW CG 8 BU Leeches (Hirudinea) Snails (Gastropoda) Clams (Bivalvia) Isopods (Isopoda) Asellidae 3 42 A AF BVW CG 8 SP Scuds (Amphipoda) Crayfish Shrimp (Decapoda) Water Mites (Hydracarina) Dragonflies (Odonata Anisoptera) Damselflies (Odonata Zygoptera) Mayflies (Ephemeroptera) Stoneflies (Plecoptera) Perlidae 2 28 I AF BVW PR 1 CR Caddisflies (Trichoptera) True Bugs (Hemiptera) Corixidae 1 14 I AF BVW MP 9 DV Dobsonflies (Megaloptera) Beetles (Coleoptera) Dytiscidae 1 14 I AF BVW PR 6 GN True Flies (Diptera) Chironomidae 61 847 I AF BVW CG 6 BU Simuliidae 3 42 I AF BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
ee
ff
Other Total Individuals 72 1001 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-4 STATION NAME Unnamed Tributary LAT (upstream) N 39deg 13035rsquo LONG (upstream) W 078deg 05129rsquo LAT (downstream) N 39deg 13000rsquo LONG (downstream) W 078deg 5086rsquo FORM COMPILED BY Andrew G M Fisher W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson A Fisher H Kepple
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Flatworms (Turbellaria) Worms (Oligochaeta) Lumbriculidae 2 32 A AF BVW CG 8 BU Leeches (Hirudinea) Snails (Gastropoda) Lymnaeidae 1 16 A BVW CG 9 GN Clams (Bivalvia) Isopods (Isopoda) Scuds (Amphipoda) Crayfish Shrimp (Decapoda) Cambaridae 1 16 A AF BVW GN 5 GN Water Mites (Hydracarina) Dragonflies (Odonata Anisoptera) Damselflies (Odonata Zygoptera) Calopterygidae 4 63 I BVW PR 7 CL Mayflies (Ephemeroptera) Tricorythidae 1 16 I BVW CG 5 SP Stoneflies (Plecoptera) Caddisflies (Trichoptera) Phryganeidae 4 63 I AF BVW SH 4 CR Hydropsychidae 6 95 I AF BVW CF 6 CG I True Bugs (Hemiptera) Corixidae 8 127 I AF BVW MP 9 DV (Veliidae ) (7) - I AF BVW ( ) Dobsonflies (Megaloptera) Sialidae 3 48 I AF BVW PR 7 SP Corydalidae 2 32 I AF BVW PR 5 CR Beetles (Coleoptera) Haliplidae 1 16 A AF BVW MP 8 GN Dysticidae 7 111 I AF BVW PR 6 GN Hydrophilidae 1 16 A AF BVW PR 8 GN Elmidae 3 48 I BVW SC 4 CG True Flies (Diptera) Chironomidae 8 127 I AF BVW CG 6 BU Simuliidae 7 111 I AF BVW CF 6 CG Tipulidae 4 63 I AF BVW SH 4 GU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
gg
hh
Other Total Individuals 63
(70) 1000
Total Taxa 17 (18)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Veliidae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-5 STATION NAME Lick Run LAT N 39deg 12752rsquo (upstream) LONG W 078deg 05685rsquo (upstream) LAT N 39deg 12784rsquo (downstream) LONG W 078deg 05631rsquo (downstream) FORM COMPILED BY Brandon Millholland W Bousquet RIVER BASIN Potomac River INVESTIGATORS J Pangle S Robertson W Bousquet JTovar M Dahl
Collection date 29 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Gomphidae 1 15 I BVW PR 3 BU Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Leptophlebiidae 11 167 A LBM BVW CG 2 CR Plecoptera (stoneflies) Perlidae 10 152 A LBM BVW PR 1 CR Perlodiae 1 15 A LBM BVW PR 2 CR Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 5 76 A LBM BVW SC 4 CG Elmidae 2 30 I LBM BVW SC 4 CG Diptera (true flies) (Culicidae) (3) - P LMB BVW ( ) Chironomidae 23 348 I LBM BVW CG 6 BU Simuliidae 13 197 I LBM BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
ii
jj
Other Total Individuals 66
(69) 1000
Total Taxa 7 (8)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Culicidae (Diptera) pupae not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl J Pangle S Robertson W Bousquet B Millholland J Tovar H Kepple
Collection date 30 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 1 12 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Asellidae 114 965 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Plecoptera (stoneflies) Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 2 23 A MRD BVW SC 4 CG Diptera (true flies)
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
kk
ll
Other Total Individuals 117 1000 Total Taxa 3
Notes Subsample taken 29 October 2009 All taxonomic determinations by MRD were confirmed by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson K McCann A Fisher H Kepple A Barrett
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 2 17 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Sphaeriidae 2 17 I MRD BVW CF 8 BU Isopoda (isopods) Asellidae 99 853 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Calopterygidae 2 17 A MRD BVW PR 7 CL Ephemeroptera (mayflies) Leptophlebiidae 2 17 I MRD BVW CG 2 CR Heptageniidae 4 34 A MRD BVW SC 3 CG Plecoptera (stoneflies) Perlidae 1 09 A MRD BVW PR 1 CR Trichoptera (caddisflies) Hemiptera (true bugs) (Gerridae) (1) - A MRD BVW ( ) Corixidae 1 09 A MRD BVW PR 5 CR Megaloptera (dobsonflies etc) Coleoptera (beetles) Dryopidae 1 09 A MRD BVW SC 5 CG Diptera (true flies) Tipulidae 2 17 I BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
mm
nn
Other Total Individuals 116
(117) 999
Total Taxa 10 (11)
Notes Subsample taken 6 November 2008 Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009 Gerridae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
- Disclaimer ii
- Title Page iii
- Acknowledgements v
- Table of Contents vii
- SECTION 2 RESULTS DATA TABLES 7
- OVERVIEW AND FINDINGS
- Introduction
- RESULTS DATA TABLES
- REFERENCES
- FIELD DATA SHEETS
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- SubstrateType
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- WATER QUALITY ANALYSIS
- THROUGH THE VIRGINIA
- STREAM CONDITION INDEX (VSCI)
- LABORATORY BENCH SHEETS
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
Environmental Studies Department Shenandoah University SITE CHARACTERIZATION FIELD DATA SHEET
FOR BENTHIC MACROINVERTEBRATE MONITORING
STREAM NAME Hiatt-Lick Run LOCATION McCann Farm STATION HLR-6 STREAM CLASS LAT N 39 13076 (upstream) LONG W 078 06539 (upstream) LAT N 39 13063 (downstream) LONG W 078 06473 (downstream) FORM COMPILED BY MDahl RIVER BASIN Potomac River INVESTIGATORS MDahl JPangle SRobertson W Bousqet BMillholland JTovar H Kepple
DATE 30 May 2008 TIME 1050 AM (start)
PROJECT NAME
WEATHER CONDITIONS
Now Past 24 hours Has there been a heavy rain in the last 7 days storm (heavy rain) YES x NO
rain (steady rain) Air Temperature __18___degC showers (intermittent) Comments _______________________
_85_x cloud cover _10__ ______________________________
clearsunny __________________________________ SITE LOCATION MAP
Draw a map of the site and indicate the areas sampled (or attach a photograph)
STREAM CHARACTER- IZATION
Stream Subsystem Stream Type x Perennial Intermittent Tidal Coldwater x Warmwater Stream Origin Catchment Area _____ km2 mi2 Montane x (Significant springs andor seeps) x ( Valley) Marsh or swamp Montane amp Valley x Mixture of origins Other ___________________
COMMENTS Multi-habitat sampling with D-frame nets and seine
m
n
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial x No evidence Some evidence x FieldPasture Industrial Obvious sources x Agricultural Other ____________ Minor stream bank scour Residential Local Watershed Erosion x None Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left 35 bank grasses scattered trees and some bushes Right High bank with grasses and scattered trees Indicate the dominant vegetation type(s) and record the dominant species
x Trees Shrubs Grasses Forbs Dominant species Black Walnut and Festuca elatior
INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width __2___m Fully open Partly open x Partly shaded Fully shaded Sampling reach area __200_m2
Estimated stream depth __03_m Proportion of Reach Represented by Stream High water mark __1___m Morphology Types Surface velocity (at thalweg) __166_msec x Riffle__0___ x Run__55___ x Pool__45___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___2____ m2 Density of LWD ___-____ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __20_____ x Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present ____Glyceria (grass)sp__________
WATER QUALITY Temperature __16___degC Water Odors x NormalNone Sewage Specific Conductance ___-___ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ____-____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used ______NA___ Clear x Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other__NONE__ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 0 Detritus sticks wood coarse plant materials
5 Boulder gt256 mm (10rsquorsquo) 0
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 27 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 38 Sand 006-2 mm (gritty) 5 Marl grey shell
fragments 0 (lt1)
Silt 0004-006 mm 25 Clay lt 0004 mm (slick) 5 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Appendix B
WATER QUALITY ANALYSIS THROUGH THE VIRGINIA
STREAM CONDITION INDEX (VSCI)
The Virginia Stream Condition Index (VSCI) relies on eight metrics to categorize stream health According to Burton and Gerritsen (2003 p 1-1) this index ldquohellip can be used as a primary indicator of ecosystem health and can identify impairment with respect to the reference (or natural) conditionrdquo These metrics are numeric measures of a biological communityrsquos species composition diversity ecological niches or tolerance to pollution
Table 13 on the next page describes each VSCI metric lists its formula and provides the best standard value (BSV) for that metric Based on a 100-point scale the BSV is the score that reflects optimum conditions for aquatic life ie the best water quality value possible (DEQ 2006b pp 8 33) The table also explains whether the score increases or decreases with environmental stress
As Table 13 indicates each of the eight VSCI metrics is calculated individually Each formula provides a unitless score based on a 100-point scale with 0 as the worst possible score and 100 as the best Any metric that provides a result above 100 is changed to 100 in order for the index to work properly After the eight metrics are calculated they are summed and averaged to provide a final VSCI score on a 100-point scale
Based on statistical analysis of Virginiarsquos streams and their BMI communities the DEQ (2006b p 8) currently uses the following tiers to interpret VSCI scores
VSCI Scores Aquatic Life Use
(ALI) Tiers
lt 42 severely stressed
42-55 moderately stressed
55-63 unknownfair (gray zone)
63-73 good
gt73 excellent
Section 2 of this report presents the results of calculations for the six BMI sampling sites the research team studied in the Hiatt-Lick Run subwatershed in 2008
o
p
1 Total taxa the number of distinct taxa in the sample measures the overall diversity of the macroinvertebrate assemblage The best standard value (BSV) is 189 Score = 100 x (X189) Total taxa decreases with stress 2 EPT taxa the sum of the distinct taxa in the orders Ephemeroptera (mayflies) Plecoptera (stoneflies) and Trichoptera (caddisflies) These orders are known to be sensitive to pollution The best standard value (BSV) is 117 Score = 100 x (X117) EPT taxa decreases with stress 3 Percent Ephemeroptera the percent abundance of individuals in the sample that belong to the order Ephemeroptera (mayflies) The best standard value (BSV) is 477 Score = 100 x (X477) Percent Ephemeroptera decreases with stress 4 Percent Plecoptera + Trichoptera less Hydropsychidae the percent abundance of individuals in the sample that belong to the orders Plecoptera (stoneflies) and Trichoptera (caddisflies) excluding the pollution-tolerant caddisfly family Hydropsychidae The best standard value (BSV) is 562 Score = 100 x (X562) Percent Plecoptera + Trichoptera less Hydropsychidae decreases with stress 5 Percent Scrapers the percent abundance of individuals in the sample whose primary functional mechanism for feeding is grazing on the substrate or periphyton (ie attached algae and associated material) The best standard value (BSV) is 467 Score = 100 x (X467) Percent scrapers decreases with stress 6 Percent Chironomidae the percent abundance of individuals in the sample that are Chironomidae (non-biting midge) larvae and pupae The best standard value (BSV) is 16 Score = 100 x [(100-X)(100-16)] Percent Chironomidae increases with stress 7 Percent Top Two Dominant the percent abundance of the two most frequent taxa present in the sample The best standard value (BSV) is 328 Score = 100 x [(100-X)(100-328)] The Percent Top 2 Dominant increases with stress 8 Hilsenhoff Biotic Index (HBI) an abundance-weighted average pollution tolerance of all the individuals within a sample keyed to the family level Tolerance ratings range from 1 (highly intolerant) to 10 (highly tolerant) The best standard value (BSV) is 29 Score = 100 x [(10-X)(10-29)] The Hilsenhoff Biotic Index (HBI) increases with stress ______________________________________________________________________________ Table 13 Description of Benthic Macroinvertebrate VSCI Metrics Includes responses (increases decreases) of each VSCI metric to degraded water quality conditions Adapted from Burton and Gerritsen (2003 pp 3-15 3-16 6-2) and DEQ (2008)
q
Appendix C
LABORATORY BENCH SHEETS
Shenandoah Universityrsquos Environmental Studies Department developed the Benthic Macroinvertebrate Laboratory Bench Sheet and VSCI Worksheet to record BMI specimen identifications and their VSCI classifications for each sample
This form is based in part on bench sheets developed by the EPA and Virginia DEQ for recording specimen identifications Additional columns on Shenandoah Universityrsquos forms for relative numbers and VSCI classifications make the calculation and verification of VSCI scores easier
Note the column for taxonomistrsquos initials Specimens were identified by students in Shenandoah Universityrsquos research group and then verified or where necessary changed by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality This external review helps assure the validity of the study Originals of these bench sheets with handwritten taxonomistsrsquo initials will be kept on file at Shenandoah Universityrsquos Environmental Studies Department for five years after the completion of this study
r
s
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-1 STATION NAME Mouth LAT N 39deg 12749rsquo (upstream) LONG W 078deg 04209rsquo (upstream) LAT N 39deg 12729rsquo (downstream) LONG W 078deg 04148rsquo (downstream) FORM COMPILED BY Jeremy Tovar W S Bousquet RIVER BASIN Potomac River INVESTIGATORS Marie Dahl Brandon Millholand J W Pangle Sean Robertson Jeremy Tovar Woodward Bousquet Ben Bousquet
Collection date 21 May 2008 Sheet completion date 12 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Hirudinea 3 33 JT BVW PR 8 SP Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Coenagrionidae 2 22 BVW PR 9 CR Ephemeroptera (mayflies) Caenidae 1 11 JT BVW CG 6 SP Ephemerllidae 4 44 JT BVW CG 2 CR Heptageniidae 5 55 JT BVW SC 3 CG Isonychiidae 1 11 BVW CF 3 CG Plecoptera (stoneflies) Perlidae 14 154 JT BVW PR 2 CR Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Corydalidae 1 11 JT BVW PR 5 CR Coleoptera (beetles) Elmidae 24 264 I JT BVW SC 4 CG Dytiscidae 4 44 JT BVW PR 6 GN Psephenidae 3 33 JT BVW SC 4 CG Diptera (true flies) Tipulidae 3 33 JT BVW SH 4 BU Chironomidae 26 286 JT BVW CG 6 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
t
Other Total Individuals 91 1001 Total Taxa 13
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
u
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-1 STATION NAME Mouth LAT N 39deg 12749rsquo (upstream) LONG W 078deg 04209rsquo (upstream) LAT N 39deg 12729rsquo (downstream) LONG W 078deg 04148rsquo (downstream) FORM COMPILED BY Jeremy Tovar W S Bousquet RIVER BASIN Potomac River INVESTIGATORS Brandon Millholand JW Pangle Jeremy Tovar Heather Kepple
Collection date 10 October 2008 Sheet completion date 12 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Oligochaeta 3 35 JT BVW CG 8 BU Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Coenagrionidae 2 23 JT BVW PR 9 CR Ephemeroptera (mayflies) Heptageniidae 12 140 JT BVW SC 3 CG Isonychiidae 2 23 BVW CF 3 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Hydropsychidae 16 186 JT BVW CF 6 CG Philopotamidae 31 360 JT BVW CF 2 CG Hemiptera (true bugs) (Veliidae) (5) - JT BVW ( ) Megaloptera (dobsonflies etc) Corydalidae 10 116 JT BVW PR 5 CR Coleoptera (beetles) Dytiscidae 1 12 JT BVW PR 6 GN Psephenidae 7 81 JT BVW SC 4 CG Diptera (true flies) Tipulidae 2 23 BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
v
Other Total Individuals 86
(91) 999
Total Taxa 10 (11)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009 Veliidae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
w
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-2 STATION NAME Package Plant LAT N 39deg 12958rsquo (upstream) LONG W 078deg 04900 (upstream) LAT N 39deg 12958rsquo (downstream) LONG W 078deg 04822 (downstream) FORM COMPILED BY JW Pangle W Bousquet RIVER BASIN Potomac River INVESTIGATORS JW Pangle S Robertson J Tovar M Dahl B Millholland W Bousquet
Collection date 29 May 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Plecoptera (stoneflies) Perlidae 11 136 I JP BVW PR 1 CR Trichoptera (caddisflies) Hydropsychidae 10 123 I JP BVW CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 14 173 I JP BVW SC 4 CG Diptera (true flies) Chironomidae 32 395 I JP BVW CG 6 BU Simuliidae 14 173 I JP BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
x
Other Total Individuals 81 1000 Total Taxa 5
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-2 STATION NAME Package Plant LAT N 39deg 12958rsquo (upstream) LONG W 078deg 04900 (upstream) LAT N 39deg 12958rsquo (downstream) LONG W 078deg 04822 (downstream) FORM COMPILED BY JW Pangle W Bousquet RIVER BASIN Potomac River INVESTIGATORS JW Pangle Brandon Millholland Jeremy Tovar Heather Kepple
Collection date 10 October 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Heptageniidae 40 392 I WBJP SC 3 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Hydropsychidae 7 69 I JP WB BVW CF 6 CG Philopotamidae 28 275 I JP WB BVW CF 2 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Corydalidae 6 59 I JP WB BVW PR 5 CR Coleoptera (beetles) Psephenidae 7 69 I JP WB BVW SC 4 CG Elmidae 8 78 I JP WB BVW SC 4 CG Diptera (true flies) Tipulidae 6 59 I JP WB BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
y
z
Other Total Individuals 102 1001 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-3 STATION NAME Rt 664 Bridge LAT N 39deg 12934rsquo (upstream) LONG W 078deg 05019rsquo (upstream) LAT N 39deg 12951rsquo (downstream) LONG W 078deg 04958rsquo (downstream) FORM COMPILED BY S Robertson W Bousquet RIVER BASIN Potomac River INVESTIGATORS SRobertson BMillholland WBousquet MDahl JPangle
Collection date 28 May 2008 Sheet completion date 11 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Leptophlebiidae 1 15 I SGR BVW CG 2 CR Baetidae 1 15 I BVW CG 5 CG Plecoptera (stoneflies) Perlidae 3 46 I SGR BVW PR 1 CR Nemouridae 1 15 I BVW SH 3 CR Trichoptera (caddisflies) Hydropsychidae 5 77 I SGR BVW CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 27 415 I SGR BVW SC 4 CG Psephenidae 2 31 A SGR BVW SC 4 CG Diptera (true flies) Simuliidae 5 77 I SGR BVW CF 6 CG Chironomidae 20 308 I SGR BVW CG 6 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
aa
bb
Other Total Individuals 65 999 Total Taxa 9
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-3 STATION NAME Rt 664 Bridge LAT N 39deg 12934rsquo (upstream) LONG W 078deg 05019rsquo (upstream) LAT N 39deg 12951rsquo (downstream) LONG W 078deg 04958rsquo (downstream) FORM COMPILED BY S Robertson RIVER BASIN Potomac River INVESTIGATORS S Robertson M Dahl A Fisher H Kepple
Collection date 10 Oct 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Heptageniidae 2 20 I SGR SC 3 CG Isonychiidae 2 20 I BVW CF 2 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Philopotamidae 36 367 I SGR CF 2 CG Hydropsychidae 47 480 I SGR CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 2 20 A SGR SC 4 CG Psephenidae 8 82 A SGR SC 4 CG Diptera (true flies) Tabanidae 1 10 I SGR PR 7 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
cc
dd
Other Total Individuals 98 999 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-4 STATION NAME Unnamed Tributary LAT (upstream) N 39deg 13035rsquo LONG (upstream) W 078deg 05129rsquo LAT (downstream) N 39deg 13000rsquo LONG (downstream) W 078deg 5086rsquo FORM COMPILED BY A Fisher M Dahl rev W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson A Fisher H Kepple
Collection date 31 May 2008 Sheet completion date rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Flatworms (Turbellaria) Worms (Oligochaeta) Naididae 1 14 - BVW CG 8 BU Leeches (Hirudinea) Snails (Gastropoda) Clams (Bivalvia) Isopods (Isopoda) Asellidae 3 42 A AF BVW CG 8 SP Scuds (Amphipoda) Crayfish Shrimp (Decapoda) Water Mites (Hydracarina) Dragonflies (Odonata Anisoptera) Damselflies (Odonata Zygoptera) Mayflies (Ephemeroptera) Stoneflies (Plecoptera) Perlidae 2 28 I AF BVW PR 1 CR Caddisflies (Trichoptera) True Bugs (Hemiptera) Corixidae 1 14 I AF BVW MP 9 DV Dobsonflies (Megaloptera) Beetles (Coleoptera) Dytiscidae 1 14 I AF BVW PR 6 GN True Flies (Diptera) Chironomidae 61 847 I AF BVW CG 6 BU Simuliidae 3 42 I AF BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
ee
ff
Other Total Individuals 72 1001 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-4 STATION NAME Unnamed Tributary LAT (upstream) N 39deg 13035rsquo LONG (upstream) W 078deg 05129rsquo LAT (downstream) N 39deg 13000rsquo LONG (downstream) W 078deg 5086rsquo FORM COMPILED BY Andrew G M Fisher W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson A Fisher H Kepple
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Flatworms (Turbellaria) Worms (Oligochaeta) Lumbriculidae 2 32 A AF BVW CG 8 BU Leeches (Hirudinea) Snails (Gastropoda) Lymnaeidae 1 16 A BVW CG 9 GN Clams (Bivalvia) Isopods (Isopoda) Scuds (Amphipoda) Crayfish Shrimp (Decapoda) Cambaridae 1 16 A AF BVW GN 5 GN Water Mites (Hydracarina) Dragonflies (Odonata Anisoptera) Damselflies (Odonata Zygoptera) Calopterygidae 4 63 I BVW PR 7 CL Mayflies (Ephemeroptera) Tricorythidae 1 16 I BVW CG 5 SP Stoneflies (Plecoptera) Caddisflies (Trichoptera) Phryganeidae 4 63 I AF BVW SH 4 CR Hydropsychidae 6 95 I AF BVW CF 6 CG I True Bugs (Hemiptera) Corixidae 8 127 I AF BVW MP 9 DV (Veliidae ) (7) - I AF BVW ( ) Dobsonflies (Megaloptera) Sialidae 3 48 I AF BVW PR 7 SP Corydalidae 2 32 I AF BVW PR 5 CR Beetles (Coleoptera) Haliplidae 1 16 A AF BVW MP 8 GN Dysticidae 7 111 I AF BVW PR 6 GN Hydrophilidae 1 16 A AF BVW PR 8 GN Elmidae 3 48 I BVW SC 4 CG True Flies (Diptera) Chironomidae 8 127 I AF BVW CG 6 BU Simuliidae 7 111 I AF BVW CF 6 CG Tipulidae 4 63 I AF BVW SH 4 GU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
gg
hh
Other Total Individuals 63
(70) 1000
Total Taxa 17 (18)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Veliidae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-5 STATION NAME Lick Run LAT N 39deg 12752rsquo (upstream) LONG W 078deg 05685rsquo (upstream) LAT N 39deg 12784rsquo (downstream) LONG W 078deg 05631rsquo (downstream) FORM COMPILED BY Brandon Millholland W Bousquet RIVER BASIN Potomac River INVESTIGATORS J Pangle S Robertson W Bousquet JTovar M Dahl
Collection date 29 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Gomphidae 1 15 I BVW PR 3 BU Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Leptophlebiidae 11 167 A LBM BVW CG 2 CR Plecoptera (stoneflies) Perlidae 10 152 A LBM BVW PR 1 CR Perlodiae 1 15 A LBM BVW PR 2 CR Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 5 76 A LBM BVW SC 4 CG Elmidae 2 30 I LBM BVW SC 4 CG Diptera (true flies) (Culicidae) (3) - P LMB BVW ( ) Chironomidae 23 348 I LBM BVW CG 6 BU Simuliidae 13 197 I LBM BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
ii
jj
Other Total Individuals 66
(69) 1000
Total Taxa 7 (8)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Culicidae (Diptera) pupae not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl J Pangle S Robertson W Bousquet B Millholland J Tovar H Kepple
Collection date 30 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 1 12 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Asellidae 114 965 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Plecoptera (stoneflies) Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 2 23 A MRD BVW SC 4 CG Diptera (true flies)
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
kk
ll
Other Total Individuals 117 1000 Total Taxa 3
Notes Subsample taken 29 October 2009 All taxonomic determinations by MRD were confirmed by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson K McCann A Fisher H Kepple A Barrett
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 2 17 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Sphaeriidae 2 17 I MRD BVW CF 8 BU Isopoda (isopods) Asellidae 99 853 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Calopterygidae 2 17 A MRD BVW PR 7 CL Ephemeroptera (mayflies) Leptophlebiidae 2 17 I MRD BVW CG 2 CR Heptageniidae 4 34 A MRD BVW SC 3 CG Plecoptera (stoneflies) Perlidae 1 09 A MRD BVW PR 1 CR Trichoptera (caddisflies) Hemiptera (true bugs) (Gerridae) (1) - A MRD BVW ( ) Corixidae 1 09 A MRD BVW PR 5 CR Megaloptera (dobsonflies etc) Coleoptera (beetles) Dryopidae 1 09 A MRD BVW SC 5 CG Diptera (true flies) Tipulidae 2 17 I BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
mm
nn
Other Total Individuals 116
(117) 999
Total Taxa 10 (11)
Notes Subsample taken 6 November 2008 Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009 Gerridae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
- Disclaimer ii
- Title Page iii
- Acknowledgements v
- Table of Contents vii
- SECTION 2 RESULTS DATA TABLES 7
- OVERVIEW AND FINDINGS
- Introduction
- RESULTS DATA TABLES
- REFERENCES
- FIELD DATA SHEETS
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- SubstrateType
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- WATER QUALITY ANALYSIS
- THROUGH THE VIRGINIA
- STREAM CONDITION INDEX (VSCI)
- LABORATORY BENCH SHEETS
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
n
LOCAL WATERSHED FEATURES
Predominant Surrounding Land Use(s) Local Watershed NPS Pollution (list) x Forest Commercial x No evidence Some evidence x FieldPasture Industrial Obvious sources x Agricultural Other ____________ Minor stream bank scour Residential Local Watershed Erosion x None Moderate Heavy
RIPARIAN ZONE ( win 18 meters)
Describe riparian and bank conditions ( vegetated disturbances erosion) looking downstream Left 35 bank grasses scattered trees and some bushes Right High bank with grasses and scattered trees Indicate the dominant vegetation type(s) and record the dominant species
x Trees Shrubs Grasses Forbs Dominant species Black Walnut and Festuca elatior
INSTREAM FEATURES
Measured reach length __100___m Canopy Cover Estimated stream width __2___m Fully open Partly open x Partly shaded Fully shaded Sampling reach area __200_m2
Estimated stream depth __03_m Proportion of Reach Represented by Stream High water mark __1___m Morphology Types Surface velocity (at thalweg) __166_msec x Riffle__0___ x Run__55___ x Pool__45___ Channelized Yes x No Dam Present Yes x No
LARGE WOODY DEBRIS
LWD ___2____ m2 Density of LWD ___-____ m2km2 (LWDsampling reach area)
AQUATIC VEGETATION
Include the dominant type(s) and record the dominant species present Portion of the reach with aquatic vegetation __20_____ x Rooted emergent Rooted submergent Rooted floating Free floating Floating Algae x Attached Algae Dominant species present ____Glyceria (grass)sp__________
WATER QUALITY Temperature __16___degC Water Odors x NormalNone Sewage Specific Conductance ___-___ Petroleum Chemical Fishy Other___________________ Dissolved Oxygen ____-____ Water Surface Oils Slick Sheen Globs Flecks pH ___-___ x None Other_____________________________ Turbidity ___-____ Turbidity (if not measured) WQ Instrument(s) Used ______NA___ Clear x Slightly Turbid Turbid ________________________________ Opaque Stained Other________________
SEDIMENT AND SUBSTRATE
Odors Deposits x Normal Sewage Petroleum Sludge Sawdust Paper fiber Sand Chemical Anaerobic None Relict shells Other__NONE__ Other_____________________ Looking at stones that are not deeply Oils embedded are the undersides black in color
x Absent Slight Moderate Profuse Yes x No
INORGANIC SUBSTRATE COMPONENTS (should add up to 100)
ORGANIC SUBSTRATE COMPONENTS (does not necessarily add up to 100)
Substrate Type Diameter
Composition in Sampling
Reach Substrate
Type
Characteristic Composition in Sampling Reach
Bedrock 0 Detritus sticks wood coarse plant materials
5 Boulder gt256 mm (10rsquorsquo) 0
Cobble 64-256 mm (25rsquorsquo-10rsquorsquo) 27 Muck-mud black very fine organic
0 Gravel 2-64 mm (01rsquorsquo-25rsquorsquo) 38 Sand 006-2 mm (gritty) 5 Marl grey shell
fragments 0 (lt1)
Silt 0004-006 mm 25 Clay lt 0004 mm (slick) 5 This form is based on the Physical CharacterizationWater Quality Field Data Sheet developed by the Environmental Protection Agency for use with the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 032009
Appendix B
WATER QUALITY ANALYSIS THROUGH THE VIRGINIA
STREAM CONDITION INDEX (VSCI)
The Virginia Stream Condition Index (VSCI) relies on eight metrics to categorize stream health According to Burton and Gerritsen (2003 p 1-1) this index ldquohellip can be used as a primary indicator of ecosystem health and can identify impairment with respect to the reference (or natural) conditionrdquo These metrics are numeric measures of a biological communityrsquos species composition diversity ecological niches or tolerance to pollution
Table 13 on the next page describes each VSCI metric lists its formula and provides the best standard value (BSV) for that metric Based on a 100-point scale the BSV is the score that reflects optimum conditions for aquatic life ie the best water quality value possible (DEQ 2006b pp 8 33) The table also explains whether the score increases or decreases with environmental stress
As Table 13 indicates each of the eight VSCI metrics is calculated individually Each formula provides a unitless score based on a 100-point scale with 0 as the worst possible score and 100 as the best Any metric that provides a result above 100 is changed to 100 in order for the index to work properly After the eight metrics are calculated they are summed and averaged to provide a final VSCI score on a 100-point scale
Based on statistical analysis of Virginiarsquos streams and their BMI communities the DEQ (2006b p 8) currently uses the following tiers to interpret VSCI scores
VSCI Scores Aquatic Life Use
(ALI) Tiers
lt 42 severely stressed
42-55 moderately stressed
55-63 unknownfair (gray zone)
63-73 good
gt73 excellent
Section 2 of this report presents the results of calculations for the six BMI sampling sites the research team studied in the Hiatt-Lick Run subwatershed in 2008
o
p
1 Total taxa the number of distinct taxa in the sample measures the overall diversity of the macroinvertebrate assemblage The best standard value (BSV) is 189 Score = 100 x (X189) Total taxa decreases with stress 2 EPT taxa the sum of the distinct taxa in the orders Ephemeroptera (mayflies) Plecoptera (stoneflies) and Trichoptera (caddisflies) These orders are known to be sensitive to pollution The best standard value (BSV) is 117 Score = 100 x (X117) EPT taxa decreases with stress 3 Percent Ephemeroptera the percent abundance of individuals in the sample that belong to the order Ephemeroptera (mayflies) The best standard value (BSV) is 477 Score = 100 x (X477) Percent Ephemeroptera decreases with stress 4 Percent Plecoptera + Trichoptera less Hydropsychidae the percent abundance of individuals in the sample that belong to the orders Plecoptera (stoneflies) and Trichoptera (caddisflies) excluding the pollution-tolerant caddisfly family Hydropsychidae The best standard value (BSV) is 562 Score = 100 x (X562) Percent Plecoptera + Trichoptera less Hydropsychidae decreases with stress 5 Percent Scrapers the percent abundance of individuals in the sample whose primary functional mechanism for feeding is grazing on the substrate or periphyton (ie attached algae and associated material) The best standard value (BSV) is 467 Score = 100 x (X467) Percent scrapers decreases with stress 6 Percent Chironomidae the percent abundance of individuals in the sample that are Chironomidae (non-biting midge) larvae and pupae The best standard value (BSV) is 16 Score = 100 x [(100-X)(100-16)] Percent Chironomidae increases with stress 7 Percent Top Two Dominant the percent abundance of the two most frequent taxa present in the sample The best standard value (BSV) is 328 Score = 100 x [(100-X)(100-328)] The Percent Top 2 Dominant increases with stress 8 Hilsenhoff Biotic Index (HBI) an abundance-weighted average pollution tolerance of all the individuals within a sample keyed to the family level Tolerance ratings range from 1 (highly intolerant) to 10 (highly tolerant) The best standard value (BSV) is 29 Score = 100 x [(10-X)(10-29)] The Hilsenhoff Biotic Index (HBI) increases with stress ______________________________________________________________________________ Table 13 Description of Benthic Macroinvertebrate VSCI Metrics Includes responses (increases decreases) of each VSCI metric to degraded water quality conditions Adapted from Burton and Gerritsen (2003 pp 3-15 3-16 6-2) and DEQ (2008)
q
Appendix C
LABORATORY BENCH SHEETS
Shenandoah Universityrsquos Environmental Studies Department developed the Benthic Macroinvertebrate Laboratory Bench Sheet and VSCI Worksheet to record BMI specimen identifications and their VSCI classifications for each sample
This form is based in part on bench sheets developed by the EPA and Virginia DEQ for recording specimen identifications Additional columns on Shenandoah Universityrsquos forms for relative numbers and VSCI classifications make the calculation and verification of VSCI scores easier
Note the column for taxonomistrsquos initials Specimens were identified by students in Shenandoah Universityrsquos research group and then verified or where necessary changed by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality This external review helps assure the validity of the study Originals of these bench sheets with handwritten taxonomistsrsquo initials will be kept on file at Shenandoah Universityrsquos Environmental Studies Department for five years after the completion of this study
r
s
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-1 STATION NAME Mouth LAT N 39deg 12749rsquo (upstream) LONG W 078deg 04209rsquo (upstream) LAT N 39deg 12729rsquo (downstream) LONG W 078deg 04148rsquo (downstream) FORM COMPILED BY Jeremy Tovar W S Bousquet RIVER BASIN Potomac River INVESTIGATORS Marie Dahl Brandon Millholand J W Pangle Sean Robertson Jeremy Tovar Woodward Bousquet Ben Bousquet
Collection date 21 May 2008 Sheet completion date 12 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Hirudinea 3 33 JT BVW PR 8 SP Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Coenagrionidae 2 22 BVW PR 9 CR Ephemeroptera (mayflies) Caenidae 1 11 JT BVW CG 6 SP Ephemerllidae 4 44 JT BVW CG 2 CR Heptageniidae 5 55 JT BVW SC 3 CG Isonychiidae 1 11 BVW CF 3 CG Plecoptera (stoneflies) Perlidae 14 154 JT BVW PR 2 CR Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Corydalidae 1 11 JT BVW PR 5 CR Coleoptera (beetles) Elmidae 24 264 I JT BVW SC 4 CG Dytiscidae 4 44 JT BVW PR 6 GN Psephenidae 3 33 JT BVW SC 4 CG Diptera (true flies) Tipulidae 3 33 JT BVW SH 4 BU Chironomidae 26 286 JT BVW CG 6 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
t
Other Total Individuals 91 1001 Total Taxa 13
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
u
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-1 STATION NAME Mouth LAT N 39deg 12749rsquo (upstream) LONG W 078deg 04209rsquo (upstream) LAT N 39deg 12729rsquo (downstream) LONG W 078deg 04148rsquo (downstream) FORM COMPILED BY Jeremy Tovar W S Bousquet RIVER BASIN Potomac River INVESTIGATORS Brandon Millholand JW Pangle Jeremy Tovar Heather Kepple
Collection date 10 October 2008 Sheet completion date 12 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Oligochaeta 3 35 JT BVW CG 8 BU Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Coenagrionidae 2 23 JT BVW PR 9 CR Ephemeroptera (mayflies) Heptageniidae 12 140 JT BVW SC 3 CG Isonychiidae 2 23 BVW CF 3 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Hydropsychidae 16 186 JT BVW CF 6 CG Philopotamidae 31 360 JT BVW CF 2 CG Hemiptera (true bugs) (Veliidae) (5) - JT BVW ( ) Megaloptera (dobsonflies etc) Corydalidae 10 116 JT BVW PR 5 CR Coleoptera (beetles) Dytiscidae 1 12 JT BVW PR 6 GN Psephenidae 7 81 JT BVW SC 4 CG Diptera (true flies) Tipulidae 2 23 BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
v
Other Total Individuals 86
(91) 999
Total Taxa 10 (11)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009 Veliidae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
w
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-2 STATION NAME Package Plant LAT N 39deg 12958rsquo (upstream) LONG W 078deg 04900 (upstream) LAT N 39deg 12958rsquo (downstream) LONG W 078deg 04822 (downstream) FORM COMPILED BY JW Pangle W Bousquet RIVER BASIN Potomac River INVESTIGATORS JW Pangle S Robertson J Tovar M Dahl B Millholland W Bousquet
Collection date 29 May 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Plecoptera (stoneflies) Perlidae 11 136 I JP BVW PR 1 CR Trichoptera (caddisflies) Hydropsychidae 10 123 I JP BVW CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 14 173 I JP BVW SC 4 CG Diptera (true flies) Chironomidae 32 395 I JP BVW CG 6 BU Simuliidae 14 173 I JP BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
x
Other Total Individuals 81 1000 Total Taxa 5
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-2 STATION NAME Package Plant LAT N 39deg 12958rsquo (upstream) LONG W 078deg 04900 (upstream) LAT N 39deg 12958rsquo (downstream) LONG W 078deg 04822 (downstream) FORM COMPILED BY JW Pangle W Bousquet RIVER BASIN Potomac River INVESTIGATORS JW Pangle Brandon Millholland Jeremy Tovar Heather Kepple
Collection date 10 October 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Heptageniidae 40 392 I WBJP SC 3 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Hydropsychidae 7 69 I JP WB BVW CF 6 CG Philopotamidae 28 275 I JP WB BVW CF 2 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Corydalidae 6 59 I JP WB BVW PR 5 CR Coleoptera (beetles) Psephenidae 7 69 I JP WB BVW SC 4 CG Elmidae 8 78 I JP WB BVW SC 4 CG Diptera (true flies) Tipulidae 6 59 I JP WB BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
y
z
Other Total Individuals 102 1001 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-3 STATION NAME Rt 664 Bridge LAT N 39deg 12934rsquo (upstream) LONG W 078deg 05019rsquo (upstream) LAT N 39deg 12951rsquo (downstream) LONG W 078deg 04958rsquo (downstream) FORM COMPILED BY S Robertson W Bousquet RIVER BASIN Potomac River INVESTIGATORS SRobertson BMillholland WBousquet MDahl JPangle
Collection date 28 May 2008 Sheet completion date 11 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Leptophlebiidae 1 15 I SGR BVW CG 2 CR Baetidae 1 15 I BVW CG 5 CG Plecoptera (stoneflies) Perlidae 3 46 I SGR BVW PR 1 CR Nemouridae 1 15 I BVW SH 3 CR Trichoptera (caddisflies) Hydropsychidae 5 77 I SGR BVW CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 27 415 I SGR BVW SC 4 CG Psephenidae 2 31 A SGR BVW SC 4 CG Diptera (true flies) Simuliidae 5 77 I SGR BVW CF 6 CG Chironomidae 20 308 I SGR BVW CG 6 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
aa
bb
Other Total Individuals 65 999 Total Taxa 9
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-3 STATION NAME Rt 664 Bridge LAT N 39deg 12934rsquo (upstream) LONG W 078deg 05019rsquo (upstream) LAT N 39deg 12951rsquo (downstream) LONG W 078deg 04958rsquo (downstream) FORM COMPILED BY S Robertson RIVER BASIN Potomac River INVESTIGATORS S Robertson M Dahl A Fisher H Kepple
Collection date 10 Oct 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Heptageniidae 2 20 I SGR SC 3 CG Isonychiidae 2 20 I BVW CF 2 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Philopotamidae 36 367 I SGR CF 2 CG Hydropsychidae 47 480 I SGR CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 2 20 A SGR SC 4 CG Psephenidae 8 82 A SGR SC 4 CG Diptera (true flies) Tabanidae 1 10 I SGR PR 7 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
cc
dd
Other Total Individuals 98 999 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-4 STATION NAME Unnamed Tributary LAT (upstream) N 39deg 13035rsquo LONG (upstream) W 078deg 05129rsquo LAT (downstream) N 39deg 13000rsquo LONG (downstream) W 078deg 5086rsquo FORM COMPILED BY A Fisher M Dahl rev W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson A Fisher H Kepple
Collection date 31 May 2008 Sheet completion date rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Flatworms (Turbellaria) Worms (Oligochaeta) Naididae 1 14 - BVW CG 8 BU Leeches (Hirudinea) Snails (Gastropoda) Clams (Bivalvia) Isopods (Isopoda) Asellidae 3 42 A AF BVW CG 8 SP Scuds (Amphipoda) Crayfish Shrimp (Decapoda) Water Mites (Hydracarina) Dragonflies (Odonata Anisoptera) Damselflies (Odonata Zygoptera) Mayflies (Ephemeroptera) Stoneflies (Plecoptera) Perlidae 2 28 I AF BVW PR 1 CR Caddisflies (Trichoptera) True Bugs (Hemiptera) Corixidae 1 14 I AF BVW MP 9 DV Dobsonflies (Megaloptera) Beetles (Coleoptera) Dytiscidae 1 14 I AF BVW PR 6 GN True Flies (Diptera) Chironomidae 61 847 I AF BVW CG 6 BU Simuliidae 3 42 I AF BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
ee
ff
Other Total Individuals 72 1001 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-4 STATION NAME Unnamed Tributary LAT (upstream) N 39deg 13035rsquo LONG (upstream) W 078deg 05129rsquo LAT (downstream) N 39deg 13000rsquo LONG (downstream) W 078deg 5086rsquo FORM COMPILED BY Andrew G M Fisher W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson A Fisher H Kepple
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Flatworms (Turbellaria) Worms (Oligochaeta) Lumbriculidae 2 32 A AF BVW CG 8 BU Leeches (Hirudinea) Snails (Gastropoda) Lymnaeidae 1 16 A BVW CG 9 GN Clams (Bivalvia) Isopods (Isopoda) Scuds (Amphipoda) Crayfish Shrimp (Decapoda) Cambaridae 1 16 A AF BVW GN 5 GN Water Mites (Hydracarina) Dragonflies (Odonata Anisoptera) Damselflies (Odonata Zygoptera) Calopterygidae 4 63 I BVW PR 7 CL Mayflies (Ephemeroptera) Tricorythidae 1 16 I BVW CG 5 SP Stoneflies (Plecoptera) Caddisflies (Trichoptera) Phryganeidae 4 63 I AF BVW SH 4 CR Hydropsychidae 6 95 I AF BVW CF 6 CG I True Bugs (Hemiptera) Corixidae 8 127 I AF BVW MP 9 DV (Veliidae ) (7) - I AF BVW ( ) Dobsonflies (Megaloptera) Sialidae 3 48 I AF BVW PR 7 SP Corydalidae 2 32 I AF BVW PR 5 CR Beetles (Coleoptera) Haliplidae 1 16 A AF BVW MP 8 GN Dysticidae 7 111 I AF BVW PR 6 GN Hydrophilidae 1 16 A AF BVW PR 8 GN Elmidae 3 48 I BVW SC 4 CG True Flies (Diptera) Chironomidae 8 127 I AF BVW CG 6 BU Simuliidae 7 111 I AF BVW CF 6 CG Tipulidae 4 63 I AF BVW SH 4 GU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
gg
hh
Other Total Individuals 63
(70) 1000
Total Taxa 17 (18)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Veliidae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-5 STATION NAME Lick Run LAT N 39deg 12752rsquo (upstream) LONG W 078deg 05685rsquo (upstream) LAT N 39deg 12784rsquo (downstream) LONG W 078deg 05631rsquo (downstream) FORM COMPILED BY Brandon Millholland W Bousquet RIVER BASIN Potomac River INVESTIGATORS J Pangle S Robertson W Bousquet JTovar M Dahl
Collection date 29 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Gomphidae 1 15 I BVW PR 3 BU Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Leptophlebiidae 11 167 A LBM BVW CG 2 CR Plecoptera (stoneflies) Perlidae 10 152 A LBM BVW PR 1 CR Perlodiae 1 15 A LBM BVW PR 2 CR Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 5 76 A LBM BVW SC 4 CG Elmidae 2 30 I LBM BVW SC 4 CG Diptera (true flies) (Culicidae) (3) - P LMB BVW ( ) Chironomidae 23 348 I LBM BVW CG 6 BU Simuliidae 13 197 I LBM BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
ii
jj
Other Total Individuals 66
(69) 1000
Total Taxa 7 (8)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Culicidae (Diptera) pupae not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl J Pangle S Robertson W Bousquet B Millholland J Tovar H Kepple
Collection date 30 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 1 12 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Asellidae 114 965 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Plecoptera (stoneflies) Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 2 23 A MRD BVW SC 4 CG Diptera (true flies)
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
kk
ll
Other Total Individuals 117 1000 Total Taxa 3
Notes Subsample taken 29 October 2009 All taxonomic determinations by MRD were confirmed by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson K McCann A Fisher H Kepple A Barrett
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 2 17 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Sphaeriidae 2 17 I MRD BVW CF 8 BU Isopoda (isopods) Asellidae 99 853 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Calopterygidae 2 17 A MRD BVW PR 7 CL Ephemeroptera (mayflies) Leptophlebiidae 2 17 I MRD BVW CG 2 CR Heptageniidae 4 34 A MRD BVW SC 3 CG Plecoptera (stoneflies) Perlidae 1 09 A MRD BVW PR 1 CR Trichoptera (caddisflies) Hemiptera (true bugs) (Gerridae) (1) - A MRD BVW ( ) Corixidae 1 09 A MRD BVW PR 5 CR Megaloptera (dobsonflies etc) Coleoptera (beetles) Dryopidae 1 09 A MRD BVW SC 5 CG Diptera (true flies) Tipulidae 2 17 I BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
mm
nn
Other Total Individuals 116
(117) 999
Total Taxa 10 (11)
Notes Subsample taken 6 November 2008 Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009 Gerridae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
- Disclaimer ii
- Title Page iii
- Acknowledgements v
- Table of Contents vii
- SECTION 2 RESULTS DATA TABLES 7
- OVERVIEW AND FINDINGS
- Introduction
- RESULTS DATA TABLES
- REFERENCES
- FIELD DATA SHEETS
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- SubstrateType
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- WATER QUALITY ANALYSIS
- THROUGH THE VIRGINIA
- STREAM CONDITION INDEX (VSCI)
- LABORATORY BENCH SHEETS
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
Appendix B
WATER QUALITY ANALYSIS THROUGH THE VIRGINIA
STREAM CONDITION INDEX (VSCI)
The Virginia Stream Condition Index (VSCI) relies on eight metrics to categorize stream health According to Burton and Gerritsen (2003 p 1-1) this index ldquohellip can be used as a primary indicator of ecosystem health and can identify impairment with respect to the reference (or natural) conditionrdquo These metrics are numeric measures of a biological communityrsquos species composition diversity ecological niches or tolerance to pollution
Table 13 on the next page describes each VSCI metric lists its formula and provides the best standard value (BSV) for that metric Based on a 100-point scale the BSV is the score that reflects optimum conditions for aquatic life ie the best water quality value possible (DEQ 2006b pp 8 33) The table also explains whether the score increases or decreases with environmental stress
As Table 13 indicates each of the eight VSCI metrics is calculated individually Each formula provides a unitless score based on a 100-point scale with 0 as the worst possible score and 100 as the best Any metric that provides a result above 100 is changed to 100 in order for the index to work properly After the eight metrics are calculated they are summed and averaged to provide a final VSCI score on a 100-point scale
Based on statistical analysis of Virginiarsquos streams and their BMI communities the DEQ (2006b p 8) currently uses the following tiers to interpret VSCI scores
VSCI Scores Aquatic Life Use
(ALI) Tiers
lt 42 severely stressed
42-55 moderately stressed
55-63 unknownfair (gray zone)
63-73 good
gt73 excellent
Section 2 of this report presents the results of calculations for the six BMI sampling sites the research team studied in the Hiatt-Lick Run subwatershed in 2008
o
p
1 Total taxa the number of distinct taxa in the sample measures the overall diversity of the macroinvertebrate assemblage The best standard value (BSV) is 189 Score = 100 x (X189) Total taxa decreases with stress 2 EPT taxa the sum of the distinct taxa in the orders Ephemeroptera (mayflies) Plecoptera (stoneflies) and Trichoptera (caddisflies) These orders are known to be sensitive to pollution The best standard value (BSV) is 117 Score = 100 x (X117) EPT taxa decreases with stress 3 Percent Ephemeroptera the percent abundance of individuals in the sample that belong to the order Ephemeroptera (mayflies) The best standard value (BSV) is 477 Score = 100 x (X477) Percent Ephemeroptera decreases with stress 4 Percent Plecoptera + Trichoptera less Hydropsychidae the percent abundance of individuals in the sample that belong to the orders Plecoptera (stoneflies) and Trichoptera (caddisflies) excluding the pollution-tolerant caddisfly family Hydropsychidae The best standard value (BSV) is 562 Score = 100 x (X562) Percent Plecoptera + Trichoptera less Hydropsychidae decreases with stress 5 Percent Scrapers the percent abundance of individuals in the sample whose primary functional mechanism for feeding is grazing on the substrate or periphyton (ie attached algae and associated material) The best standard value (BSV) is 467 Score = 100 x (X467) Percent scrapers decreases with stress 6 Percent Chironomidae the percent abundance of individuals in the sample that are Chironomidae (non-biting midge) larvae and pupae The best standard value (BSV) is 16 Score = 100 x [(100-X)(100-16)] Percent Chironomidae increases with stress 7 Percent Top Two Dominant the percent abundance of the two most frequent taxa present in the sample The best standard value (BSV) is 328 Score = 100 x [(100-X)(100-328)] The Percent Top 2 Dominant increases with stress 8 Hilsenhoff Biotic Index (HBI) an abundance-weighted average pollution tolerance of all the individuals within a sample keyed to the family level Tolerance ratings range from 1 (highly intolerant) to 10 (highly tolerant) The best standard value (BSV) is 29 Score = 100 x [(10-X)(10-29)] The Hilsenhoff Biotic Index (HBI) increases with stress ______________________________________________________________________________ Table 13 Description of Benthic Macroinvertebrate VSCI Metrics Includes responses (increases decreases) of each VSCI metric to degraded water quality conditions Adapted from Burton and Gerritsen (2003 pp 3-15 3-16 6-2) and DEQ (2008)
q
Appendix C
LABORATORY BENCH SHEETS
Shenandoah Universityrsquos Environmental Studies Department developed the Benthic Macroinvertebrate Laboratory Bench Sheet and VSCI Worksheet to record BMI specimen identifications and their VSCI classifications for each sample
This form is based in part on bench sheets developed by the EPA and Virginia DEQ for recording specimen identifications Additional columns on Shenandoah Universityrsquos forms for relative numbers and VSCI classifications make the calculation and verification of VSCI scores easier
Note the column for taxonomistrsquos initials Specimens were identified by students in Shenandoah Universityrsquos research group and then verified or where necessary changed by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality This external review helps assure the validity of the study Originals of these bench sheets with handwritten taxonomistsrsquo initials will be kept on file at Shenandoah Universityrsquos Environmental Studies Department for five years after the completion of this study
r
s
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-1 STATION NAME Mouth LAT N 39deg 12749rsquo (upstream) LONG W 078deg 04209rsquo (upstream) LAT N 39deg 12729rsquo (downstream) LONG W 078deg 04148rsquo (downstream) FORM COMPILED BY Jeremy Tovar W S Bousquet RIVER BASIN Potomac River INVESTIGATORS Marie Dahl Brandon Millholand J W Pangle Sean Robertson Jeremy Tovar Woodward Bousquet Ben Bousquet
Collection date 21 May 2008 Sheet completion date 12 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Hirudinea 3 33 JT BVW PR 8 SP Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Coenagrionidae 2 22 BVW PR 9 CR Ephemeroptera (mayflies) Caenidae 1 11 JT BVW CG 6 SP Ephemerllidae 4 44 JT BVW CG 2 CR Heptageniidae 5 55 JT BVW SC 3 CG Isonychiidae 1 11 BVW CF 3 CG Plecoptera (stoneflies) Perlidae 14 154 JT BVW PR 2 CR Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Corydalidae 1 11 JT BVW PR 5 CR Coleoptera (beetles) Elmidae 24 264 I JT BVW SC 4 CG Dytiscidae 4 44 JT BVW PR 6 GN Psephenidae 3 33 JT BVW SC 4 CG Diptera (true flies) Tipulidae 3 33 JT BVW SH 4 BU Chironomidae 26 286 JT BVW CG 6 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
t
Other Total Individuals 91 1001 Total Taxa 13
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
u
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-1 STATION NAME Mouth LAT N 39deg 12749rsquo (upstream) LONG W 078deg 04209rsquo (upstream) LAT N 39deg 12729rsquo (downstream) LONG W 078deg 04148rsquo (downstream) FORM COMPILED BY Jeremy Tovar W S Bousquet RIVER BASIN Potomac River INVESTIGATORS Brandon Millholand JW Pangle Jeremy Tovar Heather Kepple
Collection date 10 October 2008 Sheet completion date 12 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Oligochaeta 3 35 JT BVW CG 8 BU Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Coenagrionidae 2 23 JT BVW PR 9 CR Ephemeroptera (mayflies) Heptageniidae 12 140 JT BVW SC 3 CG Isonychiidae 2 23 BVW CF 3 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Hydropsychidae 16 186 JT BVW CF 6 CG Philopotamidae 31 360 JT BVW CF 2 CG Hemiptera (true bugs) (Veliidae) (5) - JT BVW ( ) Megaloptera (dobsonflies etc) Corydalidae 10 116 JT BVW PR 5 CR Coleoptera (beetles) Dytiscidae 1 12 JT BVW PR 6 GN Psephenidae 7 81 JT BVW SC 4 CG Diptera (true flies) Tipulidae 2 23 BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
v
Other Total Individuals 86
(91) 999
Total Taxa 10 (11)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009 Veliidae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
w
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-2 STATION NAME Package Plant LAT N 39deg 12958rsquo (upstream) LONG W 078deg 04900 (upstream) LAT N 39deg 12958rsquo (downstream) LONG W 078deg 04822 (downstream) FORM COMPILED BY JW Pangle W Bousquet RIVER BASIN Potomac River INVESTIGATORS JW Pangle S Robertson J Tovar M Dahl B Millholland W Bousquet
Collection date 29 May 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Plecoptera (stoneflies) Perlidae 11 136 I JP BVW PR 1 CR Trichoptera (caddisflies) Hydropsychidae 10 123 I JP BVW CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 14 173 I JP BVW SC 4 CG Diptera (true flies) Chironomidae 32 395 I JP BVW CG 6 BU Simuliidae 14 173 I JP BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
x
Other Total Individuals 81 1000 Total Taxa 5
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-2 STATION NAME Package Plant LAT N 39deg 12958rsquo (upstream) LONG W 078deg 04900 (upstream) LAT N 39deg 12958rsquo (downstream) LONG W 078deg 04822 (downstream) FORM COMPILED BY JW Pangle W Bousquet RIVER BASIN Potomac River INVESTIGATORS JW Pangle Brandon Millholland Jeremy Tovar Heather Kepple
Collection date 10 October 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Heptageniidae 40 392 I WBJP SC 3 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Hydropsychidae 7 69 I JP WB BVW CF 6 CG Philopotamidae 28 275 I JP WB BVW CF 2 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Corydalidae 6 59 I JP WB BVW PR 5 CR Coleoptera (beetles) Psephenidae 7 69 I JP WB BVW SC 4 CG Elmidae 8 78 I JP WB BVW SC 4 CG Diptera (true flies) Tipulidae 6 59 I JP WB BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
y
z
Other Total Individuals 102 1001 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-3 STATION NAME Rt 664 Bridge LAT N 39deg 12934rsquo (upstream) LONG W 078deg 05019rsquo (upstream) LAT N 39deg 12951rsquo (downstream) LONG W 078deg 04958rsquo (downstream) FORM COMPILED BY S Robertson W Bousquet RIVER BASIN Potomac River INVESTIGATORS SRobertson BMillholland WBousquet MDahl JPangle
Collection date 28 May 2008 Sheet completion date 11 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Leptophlebiidae 1 15 I SGR BVW CG 2 CR Baetidae 1 15 I BVW CG 5 CG Plecoptera (stoneflies) Perlidae 3 46 I SGR BVW PR 1 CR Nemouridae 1 15 I BVW SH 3 CR Trichoptera (caddisflies) Hydropsychidae 5 77 I SGR BVW CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 27 415 I SGR BVW SC 4 CG Psephenidae 2 31 A SGR BVW SC 4 CG Diptera (true flies) Simuliidae 5 77 I SGR BVW CF 6 CG Chironomidae 20 308 I SGR BVW CG 6 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
aa
bb
Other Total Individuals 65 999 Total Taxa 9
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-3 STATION NAME Rt 664 Bridge LAT N 39deg 12934rsquo (upstream) LONG W 078deg 05019rsquo (upstream) LAT N 39deg 12951rsquo (downstream) LONG W 078deg 04958rsquo (downstream) FORM COMPILED BY S Robertson RIVER BASIN Potomac River INVESTIGATORS S Robertson M Dahl A Fisher H Kepple
Collection date 10 Oct 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Heptageniidae 2 20 I SGR SC 3 CG Isonychiidae 2 20 I BVW CF 2 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Philopotamidae 36 367 I SGR CF 2 CG Hydropsychidae 47 480 I SGR CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 2 20 A SGR SC 4 CG Psephenidae 8 82 A SGR SC 4 CG Diptera (true flies) Tabanidae 1 10 I SGR PR 7 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
cc
dd
Other Total Individuals 98 999 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-4 STATION NAME Unnamed Tributary LAT (upstream) N 39deg 13035rsquo LONG (upstream) W 078deg 05129rsquo LAT (downstream) N 39deg 13000rsquo LONG (downstream) W 078deg 5086rsquo FORM COMPILED BY A Fisher M Dahl rev W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson A Fisher H Kepple
Collection date 31 May 2008 Sheet completion date rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Flatworms (Turbellaria) Worms (Oligochaeta) Naididae 1 14 - BVW CG 8 BU Leeches (Hirudinea) Snails (Gastropoda) Clams (Bivalvia) Isopods (Isopoda) Asellidae 3 42 A AF BVW CG 8 SP Scuds (Amphipoda) Crayfish Shrimp (Decapoda) Water Mites (Hydracarina) Dragonflies (Odonata Anisoptera) Damselflies (Odonata Zygoptera) Mayflies (Ephemeroptera) Stoneflies (Plecoptera) Perlidae 2 28 I AF BVW PR 1 CR Caddisflies (Trichoptera) True Bugs (Hemiptera) Corixidae 1 14 I AF BVW MP 9 DV Dobsonflies (Megaloptera) Beetles (Coleoptera) Dytiscidae 1 14 I AF BVW PR 6 GN True Flies (Diptera) Chironomidae 61 847 I AF BVW CG 6 BU Simuliidae 3 42 I AF BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
ee
ff
Other Total Individuals 72 1001 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-4 STATION NAME Unnamed Tributary LAT (upstream) N 39deg 13035rsquo LONG (upstream) W 078deg 05129rsquo LAT (downstream) N 39deg 13000rsquo LONG (downstream) W 078deg 5086rsquo FORM COMPILED BY Andrew G M Fisher W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson A Fisher H Kepple
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Flatworms (Turbellaria) Worms (Oligochaeta) Lumbriculidae 2 32 A AF BVW CG 8 BU Leeches (Hirudinea) Snails (Gastropoda) Lymnaeidae 1 16 A BVW CG 9 GN Clams (Bivalvia) Isopods (Isopoda) Scuds (Amphipoda) Crayfish Shrimp (Decapoda) Cambaridae 1 16 A AF BVW GN 5 GN Water Mites (Hydracarina) Dragonflies (Odonata Anisoptera) Damselflies (Odonata Zygoptera) Calopterygidae 4 63 I BVW PR 7 CL Mayflies (Ephemeroptera) Tricorythidae 1 16 I BVW CG 5 SP Stoneflies (Plecoptera) Caddisflies (Trichoptera) Phryganeidae 4 63 I AF BVW SH 4 CR Hydropsychidae 6 95 I AF BVW CF 6 CG I True Bugs (Hemiptera) Corixidae 8 127 I AF BVW MP 9 DV (Veliidae ) (7) - I AF BVW ( ) Dobsonflies (Megaloptera) Sialidae 3 48 I AF BVW PR 7 SP Corydalidae 2 32 I AF BVW PR 5 CR Beetles (Coleoptera) Haliplidae 1 16 A AF BVW MP 8 GN Dysticidae 7 111 I AF BVW PR 6 GN Hydrophilidae 1 16 A AF BVW PR 8 GN Elmidae 3 48 I BVW SC 4 CG True Flies (Diptera) Chironomidae 8 127 I AF BVW CG 6 BU Simuliidae 7 111 I AF BVW CF 6 CG Tipulidae 4 63 I AF BVW SH 4 GU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
gg
hh
Other Total Individuals 63
(70) 1000
Total Taxa 17 (18)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Veliidae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-5 STATION NAME Lick Run LAT N 39deg 12752rsquo (upstream) LONG W 078deg 05685rsquo (upstream) LAT N 39deg 12784rsquo (downstream) LONG W 078deg 05631rsquo (downstream) FORM COMPILED BY Brandon Millholland W Bousquet RIVER BASIN Potomac River INVESTIGATORS J Pangle S Robertson W Bousquet JTovar M Dahl
Collection date 29 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Gomphidae 1 15 I BVW PR 3 BU Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Leptophlebiidae 11 167 A LBM BVW CG 2 CR Plecoptera (stoneflies) Perlidae 10 152 A LBM BVW PR 1 CR Perlodiae 1 15 A LBM BVW PR 2 CR Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 5 76 A LBM BVW SC 4 CG Elmidae 2 30 I LBM BVW SC 4 CG Diptera (true flies) (Culicidae) (3) - P LMB BVW ( ) Chironomidae 23 348 I LBM BVW CG 6 BU Simuliidae 13 197 I LBM BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
ii
jj
Other Total Individuals 66
(69) 1000
Total Taxa 7 (8)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Culicidae (Diptera) pupae not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl J Pangle S Robertson W Bousquet B Millholland J Tovar H Kepple
Collection date 30 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 1 12 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Asellidae 114 965 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Plecoptera (stoneflies) Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 2 23 A MRD BVW SC 4 CG Diptera (true flies)
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
kk
ll
Other Total Individuals 117 1000 Total Taxa 3
Notes Subsample taken 29 October 2009 All taxonomic determinations by MRD were confirmed by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson K McCann A Fisher H Kepple A Barrett
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 2 17 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Sphaeriidae 2 17 I MRD BVW CF 8 BU Isopoda (isopods) Asellidae 99 853 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Calopterygidae 2 17 A MRD BVW PR 7 CL Ephemeroptera (mayflies) Leptophlebiidae 2 17 I MRD BVW CG 2 CR Heptageniidae 4 34 A MRD BVW SC 3 CG Plecoptera (stoneflies) Perlidae 1 09 A MRD BVW PR 1 CR Trichoptera (caddisflies) Hemiptera (true bugs) (Gerridae) (1) - A MRD BVW ( ) Corixidae 1 09 A MRD BVW PR 5 CR Megaloptera (dobsonflies etc) Coleoptera (beetles) Dryopidae 1 09 A MRD BVW SC 5 CG Diptera (true flies) Tipulidae 2 17 I BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
mm
nn
Other Total Individuals 116
(117) 999
Total Taxa 10 (11)
Notes Subsample taken 6 November 2008 Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009 Gerridae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
- Disclaimer ii
- Title Page iii
- Acknowledgements v
- Table of Contents vii
- SECTION 2 RESULTS DATA TABLES 7
- OVERVIEW AND FINDINGS
- Introduction
- RESULTS DATA TABLES
- REFERENCES
- FIELD DATA SHEETS
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- SubstrateType
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- WATER QUALITY ANALYSIS
- THROUGH THE VIRGINIA
- STREAM CONDITION INDEX (VSCI)
- LABORATORY BENCH SHEETS
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
p
1 Total taxa the number of distinct taxa in the sample measures the overall diversity of the macroinvertebrate assemblage The best standard value (BSV) is 189 Score = 100 x (X189) Total taxa decreases with stress 2 EPT taxa the sum of the distinct taxa in the orders Ephemeroptera (mayflies) Plecoptera (stoneflies) and Trichoptera (caddisflies) These orders are known to be sensitive to pollution The best standard value (BSV) is 117 Score = 100 x (X117) EPT taxa decreases with stress 3 Percent Ephemeroptera the percent abundance of individuals in the sample that belong to the order Ephemeroptera (mayflies) The best standard value (BSV) is 477 Score = 100 x (X477) Percent Ephemeroptera decreases with stress 4 Percent Plecoptera + Trichoptera less Hydropsychidae the percent abundance of individuals in the sample that belong to the orders Plecoptera (stoneflies) and Trichoptera (caddisflies) excluding the pollution-tolerant caddisfly family Hydropsychidae The best standard value (BSV) is 562 Score = 100 x (X562) Percent Plecoptera + Trichoptera less Hydropsychidae decreases with stress 5 Percent Scrapers the percent abundance of individuals in the sample whose primary functional mechanism for feeding is grazing on the substrate or periphyton (ie attached algae and associated material) The best standard value (BSV) is 467 Score = 100 x (X467) Percent scrapers decreases with stress 6 Percent Chironomidae the percent abundance of individuals in the sample that are Chironomidae (non-biting midge) larvae and pupae The best standard value (BSV) is 16 Score = 100 x [(100-X)(100-16)] Percent Chironomidae increases with stress 7 Percent Top Two Dominant the percent abundance of the two most frequent taxa present in the sample The best standard value (BSV) is 328 Score = 100 x [(100-X)(100-328)] The Percent Top 2 Dominant increases with stress 8 Hilsenhoff Biotic Index (HBI) an abundance-weighted average pollution tolerance of all the individuals within a sample keyed to the family level Tolerance ratings range from 1 (highly intolerant) to 10 (highly tolerant) The best standard value (BSV) is 29 Score = 100 x [(10-X)(10-29)] The Hilsenhoff Biotic Index (HBI) increases with stress ______________________________________________________________________________ Table 13 Description of Benthic Macroinvertebrate VSCI Metrics Includes responses (increases decreases) of each VSCI metric to degraded water quality conditions Adapted from Burton and Gerritsen (2003 pp 3-15 3-16 6-2) and DEQ (2008)
q
Appendix C
LABORATORY BENCH SHEETS
Shenandoah Universityrsquos Environmental Studies Department developed the Benthic Macroinvertebrate Laboratory Bench Sheet and VSCI Worksheet to record BMI specimen identifications and their VSCI classifications for each sample
This form is based in part on bench sheets developed by the EPA and Virginia DEQ for recording specimen identifications Additional columns on Shenandoah Universityrsquos forms for relative numbers and VSCI classifications make the calculation and verification of VSCI scores easier
Note the column for taxonomistrsquos initials Specimens were identified by students in Shenandoah Universityrsquos research group and then verified or where necessary changed by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality This external review helps assure the validity of the study Originals of these bench sheets with handwritten taxonomistsrsquo initials will be kept on file at Shenandoah Universityrsquos Environmental Studies Department for five years after the completion of this study
r
s
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-1 STATION NAME Mouth LAT N 39deg 12749rsquo (upstream) LONG W 078deg 04209rsquo (upstream) LAT N 39deg 12729rsquo (downstream) LONG W 078deg 04148rsquo (downstream) FORM COMPILED BY Jeremy Tovar W S Bousquet RIVER BASIN Potomac River INVESTIGATORS Marie Dahl Brandon Millholand J W Pangle Sean Robertson Jeremy Tovar Woodward Bousquet Ben Bousquet
Collection date 21 May 2008 Sheet completion date 12 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Hirudinea 3 33 JT BVW PR 8 SP Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Coenagrionidae 2 22 BVW PR 9 CR Ephemeroptera (mayflies) Caenidae 1 11 JT BVW CG 6 SP Ephemerllidae 4 44 JT BVW CG 2 CR Heptageniidae 5 55 JT BVW SC 3 CG Isonychiidae 1 11 BVW CF 3 CG Plecoptera (stoneflies) Perlidae 14 154 JT BVW PR 2 CR Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Corydalidae 1 11 JT BVW PR 5 CR Coleoptera (beetles) Elmidae 24 264 I JT BVW SC 4 CG Dytiscidae 4 44 JT BVW PR 6 GN Psephenidae 3 33 JT BVW SC 4 CG Diptera (true flies) Tipulidae 3 33 JT BVW SH 4 BU Chironomidae 26 286 JT BVW CG 6 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
t
Other Total Individuals 91 1001 Total Taxa 13
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
u
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-1 STATION NAME Mouth LAT N 39deg 12749rsquo (upstream) LONG W 078deg 04209rsquo (upstream) LAT N 39deg 12729rsquo (downstream) LONG W 078deg 04148rsquo (downstream) FORM COMPILED BY Jeremy Tovar W S Bousquet RIVER BASIN Potomac River INVESTIGATORS Brandon Millholand JW Pangle Jeremy Tovar Heather Kepple
Collection date 10 October 2008 Sheet completion date 12 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Oligochaeta 3 35 JT BVW CG 8 BU Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Coenagrionidae 2 23 JT BVW PR 9 CR Ephemeroptera (mayflies) Heptageniidae 12 140 JT BVW SC 3 CG Isonychiidae 2 23 BVW CF 3 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Hydropsychidae 16 186 JT BVW CF 6 CG Philopotamidae 31 360 JT BVW CF 2 CG Hemiptera (true bugs) (Veliidae) (5) - JT BVW ( ) Megaloptera (dobsonflies etc) Corydalidae 10 116 JT BVW PR 5 CR Coleoptera (beetles) Dytiscidae 1 12 JT BVW PR 6 GN Psephenidae 7 81 JT BVW SC 4 CG Diptera (true flies) Tipulidae 2 23 BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
v
Other Total Individuals 86
(91) 999
Total Taxa 10 (11)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009 Veliidae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
w
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-2 STATION NAME Package Plant LAT N 39deg 12958rsquo (upstream) LONG W 078deg 04900 (upstream) LAT N 39deg 12958rsquo (downstream) LONG W 078deg 04822 (downstream) FORM COMPILED BY JW Pangle W Bousquet RIVER BASIN Potomac River INVESTIGATORS JW Pangle S Robertson J Tovar M Dahl B Millholland W Bousquet
Collection date 29 May 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Plecoptera (stoneflies) Perlidae 11 136 I JP BVW PR 1 CR Trichoptera (caddisflies) Hydropsychidae 10 123 I JP BVW CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 14 173 I JP BVW SC 4 CG Diptera (true flies) Chironomidae 32 395 I JP BVW CG 6 BU Simuliidae 14 173 I JP BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
x
Other Total Individuals 81 1000 Total Taxa 5
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-2 STATION NAME Package Plant LAT N 39deg 12958rsquo (upstream) LONG W 078deg 04900 (upstream) LAT N 39deg 12958rsquo (downstream) LONG W 078deg 04822 (downstream) FORM COMPILED BY JW Pangle W Bousquet RIVER BASIN Potomac River INVESTIGATORS JW Pangle Brandon Millholland Jeremy Tovar Heather Kepple
Collection date 10 October 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Heptageniidae 40 392 I WBJP SC 3 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Hydropsychidae 7 69 I JP WB BVW CF 6 CG Philopotamidae 28 275 I JP WB BVW CF 2 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Corydalidae 6 59 I JP WB BVW PR 5 CR Coleoptera (beetles) Psephenidae 7 69 I JP WB BVW SC 4 CG Elmidae 8 78 I JP WB BVW SC 4 CG Diptera (true flies) Tipulidae 6 59 I JP WB BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
y
z
Other Total Individuals 102 1001 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-3 STATION NAME Rt 664 Bridge LAT N 39deg 12934rsquo (upstream) LONG W 078deg 05019rsquo (upstream) LAT N 39deg 12951rsquo (downstream) LONG W 078deg 04958rsquo (downstream) FORM COMPILED BY S Robertson W Bousquet RIVER BASIN Potomac River INVESTIGATORS SRobertson BMillholland WBousquet MDahl JPangle
Collection date 28 May 2008 Sheet completion date 11 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Leptophlebiidae 1 15 I SGR BVW CG 2 CR Baetidae 1 15 I BVW CG 5 CG Plecoptera (stoneflies) Perlidae 3 46 I SGR BVW PR 1 CR Nemouridae 1 15 I BVW SH 3 CR Trichoptera (caddisflies) Hydropsychidae 5 77 I SGR BVW CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 27 415 I SGR BVW SC 4 CG Psephenidae 2 31 A SGR BVW SC 4 CG Diptera (true flies) Simuliidae 5 77 I SGR BVW CF 6 CG Chironomidae 20 308 I SGR BVW CG 6 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
aa
bb
Other Total Individuals 65 999 Total Taxa 9
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-3 STATION NAME Rt 664 Bridge LAT N 39deg 12934rsquo (upstream) LONG W 078deg 05019rsquo (upstream) LAT N 39deg 12951rsquo (downstream) LONG W 078deg 04958rsquo (downstream) FORM COMPILED BY S Robertson RIVER BASIN Potomac River INVESTIGATORS S Robertson M Dahl A Fisher H Kepple
Collection date 10 Oct 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Heptageniidae 2 20 I SGR SC 3 CG Isonychiidae 2 20 I BVW CF 2 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Philopotamidae 36 367 I SGR CF 2 CG Hydropsychidae 47 480 I SGR CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 2 20 A SGR SC 4 CG Psephenidae 8 82 A SGR SC 4 CG Diptera (true flies) Tabanidae 1 10 I SGR PR 7 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
cc
dd
Other Total Individuals 98 999 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-4 STATION NAME Unnamed Tributary LAT (upstream) N 39deg 13035rsquo LONG (upstream) W 078deg 05129rsquo LAT (downstream) N 39deg 13000rsquo LONG (downstream) W 078deg 5086rsquo FORM COMPILED BY A Fisher M Dahl rev W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson A Fisher H Kepple
Collection date 31 May 2008 Sheet completion date rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Flatworms (Turbellaria) Worms (Oligochaeta) Naididae 1 14 - BVW CG 8 BU Leeches (Hirudinea) Snails (Gastropoda) Clams (Bivalvia) Isopods (Isopoda) Asellidae 3 42 A AF BVW CG 8 SP Scuds (Amphipoda) Crayfish Shrimp (Decapoda) Water Mites (Hydracarina) Dragonflies (Odonata Anisoptera) Damselflies (Odonata Zygoptera) Mayflies (Ephemeroptera) Stoneflies (Plecoptera) Perlidae 2 28 I AF BVW PR 1 CR Caddisflies (Trichoptera) True Bugs (Hemiptera) Corixidae 1 14 I AF BVW MP 9 DV Dobsonflies (Megaloptera) Beetles (Coleoptera) Dytiscidae 1 14 I AF BVW PR 6 GN True Flies (Diptera) Chironomidae 61 847 I AF BVW CG 6 BU Simuliidae 3 42 I AF BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
ee
ff
Other Total Individuals 72 1001 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-4 STATION NAME Unnamed Tributary LAT (upstream) N 39deg 13035rsquo LONG (upstream) W 078deg 05129rsquo LAT (downstream) N 39deg 13000rsquo LONG (downstream) W 078deg 5086rsquo FORM COMPILED BY Andrew G M Fisher W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson A Fisher H Kepple
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Flatworms (Turbellaria) Worms (Oligochaeta) Lumbriculidae 2 32 A AF BVW CG 8 BU Leeches (Hirudinea) Snails (Gastropoda) Lymnaeidae 1 16 A BVW CG 9 GN Clams (Bivalvia) Isopods (Isopoda) Scuds (Amphipoda) Crayfish Shrimp (Decapoda) Cambaridae 1 16 A AF BVW GN 5 GN Water Mites (Hydracarina) Dragonflies (Odonata Anisoptera) Damselflies (Odonata Zygoptera) Calopterygidae 4 63 I BVW PR 7 CL Mayflies (Ephemeroptera) Tricorythidae 1 16 I BVW CG 5 SP Stoneflies (Plecoptera) Caddisflies (Trichoptera) Phryganeidae 4 63 I AF BVW SH 4 CR Hydropsychidae 6 95 I AF BVW CF 6 CG I True Bugs (Hemiptera) Corixidae 8 127 I AF BVW MP 9 DV (Veliidae ) (7) - I AF BVW ( ) Dobsonflies (Megaloptera) Sialidae 3 48 I AF BVW PR 7 SP Corydalidae 2 32 I AF BVW PR 5 CR Beetles (Coleoptera) Haliplidae 1 16 A AF BVW MP 8 GN Dysticidae 7 111 I AF BVW PR 6 GN Hydrophilidae 1 16 A AF BVW PR 8 GN Elmidae 3 48 I BVW SC 4 CG True Flies (Diptera) Chironomidae 8 127 I AF BVW CG 6 BU Simuliidae 7 111 I AF BVW CF 6 CG Tipulidae 4 63 I AF BVW SH 4 GU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
gg
hh
Other Total Individuals 63
(70) 1000
Total Taxa 17 (18)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Veliidae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-5 STATION NAME Lick Run LAT N 39deg 12752rsquo (upstream) LONG W 078deg 05685rsquo (upstream) LAT N 39deg 12784rsquo (downstream) LONG W 078deg 05631rsquo (downstream) FORM COMPILED BY Brandon Millholland W Bousquet RIVER BASIN Potomac River INVESTIGATORS J Pangle S Robertson W Bousquet JTovar M Dahl
Collection date 29 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Gomphidae 1 15 I BVW PR 3 BU Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Leptophlebiidae 11 167 A LBM BVW CG 2 CR Plecoptera (stoneflies) Perlidae 10 152 A LBM BVW PR 1 CR Perlodiae 1 15 A LBM BVW PR 2 CR Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 5 76 A LBM BVW SC 4 CG Elmidae 2 30 I LBM BVW SC 4 CG Diptera (true flies) (Culicidae) (3) - P LMB BVW ( ) Chironomidae 23 348 I LBM BVW CG 6 BU Simuliidae 13 197 I LBM BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
ii
jj
Other Total Individuals 66
(69) 1000
Total Taxa 7 (8)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Culicidae (Diptera) pupae not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl J Pangle S Robertson W Bousquet B Millholland J Tovar H Kepple
Collection date 30 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 1 12 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Asellidae 114 965 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Plecoptera (stoneflies) Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 2 23 A MRD BVW SC 4 CG Diptera (true flies)
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
kk
ll
Other Total Individuals 117 1000 Total Taxa 3
Notes Subsample taken 29 October 2009 All taxonomic determinations by MRD were confirmed by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson K McCann A Fisher H Kepple A Barrett
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 2 17 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Sphaeriidae 2 17 I MRD BVW CF 8 BU Isopoda (isopods) Asellidae 99 853 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Calopterygidae 2 17 A MRD BVW PR 7 CL Ephemeroptera (mayflies) Leptophlebiidae 2 17 I MRD BVW CG 2 CR Heptageniidae 4 34 A MRD BVW SC 3 CG Plecoptera (stoneflies) Perlidae 1 09 A MRD BVW PR 1 CR Trichoptera (caddisflies) Hemiptera (true bugs) (Gerridae) (1) - A MRD BVW ( ) Corixidae 1 09 A MRD BVW PR 5 CR Megaloptera (dobsonflies etc) Coleoptera (beetles) Dryopidae 1 09 A MRD BVW SC 5 CG Diptera (true flies) Tipulidae 2 17 I BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
mm
nn
Other Total Individuals 116
(117) 999
Total Taxa 10 (11)
Notes Subsample taken 6 November 2008 Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009 Gerridae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
- Disclaimer ii
- Title Page iii
- Acknowledgements v
- Table of Contents vii
- SECTION 2 RESULTS DATA TABLES 7
- OVERVIEW AND FINDINGS
- Introduction
- RESULTS DATA TABLES
- REFERENCES
- FIELD DATA SHEETS
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- SubstrateType
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- WATER QUALITY ANALYSIS
- THROUGH THE VIRGINIA
- STREAM CONDITION INDEX (VSCI)
- LABORATORY BENCH SHEETS
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
q
Appendix C
LABORATORY BENCH SHEETS
Shenandoah Universityrsquos Environmental Studies Department developed the Benthic Macroinvertebrate Laboratory Bench Sheet and VSCI Worksheet to record BMI specimen identifications and their VSCI classifications for each sample
This form is based in part on bench sheets developed by the EPA and Virginia DEQ for recording specimen identifications Additional columns on Shenandoah Universityrsquos forms for relative numbers and VSCI classifications make the calculation and verification of VSCI scores easier
Note the column for taxonomistrsquos initials Specimens were identified by students in Shenandoah Universityrsquos research group and then verified or where necessary changed by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality This external review helps assure the validity of the study Originals of these bench sheets with handwritten taxonomistsrsquo initials will be kept on file at Shenandoah Universityrsquos Environmental Studies Department for five years after the completion of this study
r
s
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-1 STATION NAME Mouth LAT N 39deg 12749rsquo (upstream) LONG W 078deg 04209rsquo (upstream) LAT N 39deg 12729rsquo (downstream) LONG W 078deg 04148rsquo (downstream) FORM COMPILED BY Jeremy Tovar W S Bousquet RIVER BASIN Potomac River INVESTIGATORS Marie Dahl Brandon Millholand J W Pangle Sean Robertson Jeremy Tovar Woodward Bousquet Ben Bousquet
Collection date 21 May 2008 Sheet completion date 12 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Hirudinea 3 33 JT BVW PR 8 SP Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Coenagrionidae 2 22 BVW PR 9 CR Ephemeroptera (mayflies) Caenidae 1 11 JT BVW CG 6 SP Ephemerllidae 4 44 JT BVW CG 2 CR Heptageniidae 5 55 JT BVW SC 3 CG Isonychiidae 1 11 BVW CF 3 CG Plecoptera (stoneflies) Perlidae 14 154 JT BVW PR 2 CR Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Corydalidae 1 11 JT BVW PR 5 CR Coleoptera (beetles) Elmidae 24 264 I JT BVW SC 4 CG Dytiscidae 4 44 JT BVW PR 6 GN Psephenidae 3 33 JT BVW SC 4 CG Diptera (true flies) Tipulidae 3 33 JT BVW SH 4 BU Chironomidae 26 286 JT BVW CG 6 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
t
Other Total Individuals 91 1001 Total Taxa 13
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
u
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-1 STATION NAME Mouth LAT N 39deg 12749rsquo (upstream) LONG W 078deg 04209rsquo (upstream) LAT N 39deg 12729rsquo (downstream) LONG W 078deg 04148rsquo (downstream) FORM COMPILED BY Jeremy Tovar W S Bousquet RIVER BASIN Potomac River INVESTIGATORS Brandon Millholand JW Pangle Jeremy Tovar Heather Kepple
Collection date 10 October 2008 Sheet completion date 12 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Oligochaeta 3 35 JT BVW CG 8 BU Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Coenagrionidae 2 23 JT BVW PR 9 CR Ephemeroptera (mayflies) Heptageniidae 12 140 JT BVW SC 3 CG Isonychiidae 2 23 BVW CF 3 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Hydropsychidae 16 186 JT BVW CF 6 CG Philopotamidae 31 360 JT BVW CF 2 CG Hemiptera (true bugs) (Veliidae) (5) - JT BVW ( ) Megaloptera (dobsonflies etc) Corydalidae 10 116 JT BVW PR 5 CR Coleoptera (beetles) Dytiscidae 1 12 JT BVW PR 6 GN Psephenidae 7 81 JT BVW SC 4 CG Diptera (true flies) Tipulidae 2 23 BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
v
Other Total Individuals 86
(91) 999
Total Taxa 10 (11)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009 Veliidae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
w
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-2 STATION NAME Package Plant LAT N 39deg 12958rsquo (upstream) LONG W 078deg 04900 (upstream) LAT N 39deg 12958rsquo (downstream) LONG W 078deg 04822 (downstream) FORM COMPILED BY JW Pangle W Bousquet RIVER BASIN Potomac River INVESTIGATORS JW Pangle S Robertson J Tovar M Dahl B Millholland W Bousquet
Collection date 29 May 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Plecoptera (stoneflies) Perlidae 11 136 I JP BVW PR 1 CR Trichoptera (caddisflies) Hydropsychidae 10 123 I JP BVW CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 14 173 I JP BVW SC 4 CG Diptera (true flies) Chironomidae 32 395 I JP BVW CG 6 BU Simuliidae 14 173 I JP BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
x
Other Total Individuals 81 1000 Total Taxa 5
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-2 STATION NAME Package Plant LAT N 39deg 12958rsquo (upstream) LONG W 078deg 04900 (upstream) LAT N 39deg 12958rsquo (downstream) LONG W 078deg 04822 (downstream) FORM COMPILED BY JW Pangle W Bousquet RIVER BASIN Potomac River INVESTIGATORS JW Pangle Brandon Millholland Jeremy Tovar Heather Kepple
Collection date 10 October 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Heptageniidae 40 392 I WBJP SC 3 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Hydropsychidae 7 69 I JP WB BVW CF 6 CG Philopotamidae 28 275 I JP WB BVW CF 2 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Corydalidae 6 59 I JP WB BVW PR 5 CR Coleoptera (beetles) Psephenidae 7 69 I JP WB BVW SC 4 CG Elmidae 8 78 I JP WB BVW SC 4 CG Diptera (true flies) Tipulidae 6 59 I JP WB BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
y
z
Other Total Individuals 102 1001 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-3 STATION NAME Rt 664 Bridge LAT N 39deg 12934rsquo (upstream) LONG W 078deg 05019rsquo (upstream) LAT N 39deg 12951rsquo (downstream) LONG W 078deg 04958rsquo (downstream) FORM COMPILED BY S Robertson W Bousquet RIVER BASIN Potomac River INVESTIGATORS SRobertson BMillholland WBousquet MDahl JPangle
Collection date 28 May 2008 Sheet completion date 11 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Leptophlebiidae 1 15 I SGR BVW CG 2 CR Baetidae 1 15 I BVW CG 5 CG Plecoptera (stoneflies) Perlidae 3 46 I SGR BVW PR 1 CR Nemouridae 1 15 I BVW SH 3 CR Trichoptera (caddisflies) Hydropsychidae 5 77 I SGR BVW CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 27 415 I SGR BVW SC 4 CG Psephenidae 2 31 A SGR BVW SC 4 CG Diptera (true flies) Simuliidae 5 77 I SGR BVW CF 6 CG Chironomidae 20 308 I SGR BVW CG 6 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
aa
bb
Other Total Individuals 65 999 Total Taxa 9
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-3 STATION NAME Rt 664 Bridge LAT N 39deg 12934rsquo (upstream) LONG W 078deg 05019rsquo (upstream) LAT N 39deg 12951rsquo (downstream) LONG W 078deg 04958rsquo (downstream) FORM COMPILED BY S Robertson RIVER BASIN Potomac River INVESTIGATORS S Robertson M Dahl A Fisher H Kepple
Collection date 10 Oct 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Heptageniidae 2 20 I SGR SC 3 CG Isonychiidae 2 20 I BVW CF 2 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Philopotamidae 36 367 I SGR CF 2 CG Hydropsychidae 47 480 I SGR CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 2 20 A SGR SC 4 CG Psephenidae 8 82 A SGR SC 4 CG Diptera (true flies) Tabanidae 1 10 I SGR PR 7 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
cc
dd
Other Total Individuals 98 999 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-4 STATION NAME Unnamed Tributary LAT (upstream) N 39deg 13035rsquo LONG (upstream) W 078deg 05129rsquo LAT (downstream) N 39deg 13000rsquo LONG (downstream) W 078deg 5086rsquo FORM COMPILED BY A Fisher M Dahl rev W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson A Fisher H Kepple
Collection date 31 May 2008 Sheet completion date rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Flatworms (Turbellaria) Worms (Oligochaeta) Naididae 1 14 - BVW CG 8 BU Leeches (Hirudinea) Snails (Gastropoda) Clams (Bivalvia) Isopods (Isopoda) Asellidae 3 42 A AF BVW CG 8 SP Scuds (Amphipoda) Crayfish Shrimp (Decapoda) Water Mites (Hydracarina) Dragonflies (Odonata Anisoptera) Damselflies (Odonata Zygoptera) Mayflies (Ephemeroptera) Stoneflies (Plecoptera) Perlidae 2 28 I AF BVW PR 1 CR Caddisflies (Trichoptera) True Bugs (Hemiptera) Corixidae 1 14 I AF BVW MP 9 DV Dobsonflies (Megaloptera) Beetles (Coleoptera) Dytiscidae 1 14 I AF BVW PR 6 GN True Flies (Diptera) Chironomidae 61 847 I AF BVW CG 6 BU Simuliidae 3 42 I AF BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
ee
ff
Other Total Individuals 72 1001 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-4 STATION NAME Unnamed Tributary LAT (upstream) N 39deg 13035rsquo LONG (upstream) W 078deg 05129rsquo LAT (downstream) N 39deg 13000rsquo LONG (downstream) W 078deg 5086rsquo FORM COMPILED BY Andrew G M Fisher W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson A Fisher H Kepple
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Flatworms (Turbellaria) Worms (Oligochaeta) Lumbriculidae 2 32 A AF BVW CG 8 BU Leeches (Hirudinea) Snails (Gastropoda) Lymnaeidae 1 16 A BVW CG 9 GN Clams (Bivalvia) Isopods (Isopoda) Scuds (Amphipoda) Crayfish Shrimp (Decapoda) Cambaridae 1 16 A AF BVW GN 5 GN Water Mites (Hydracarina) Dragonflies (Odonata Anisoptera) Damselflies (Odonata Zygoptera) Calopterygidae 4 63 I BVW PR 7 CL Mayflies (Ephemeroptera) Tricorythidae 1 16 I BVW CG 5 SP Stoneflies (Plecoptera) Caddisflies (Trichoptera) Phryganeidae 4 63 I AF BVW SH 4 CR Hydropsychidae 6 95 I AF BVW CF 6 CG I True Bugs (Hemiptera) Corixidae 8 127 I AF BVW MP 9 DV (Veliidae ) (7) - I AF BVW ( ) Dobsonflies (Megaloptera) Sialidae 3 48 I AF BVW PR 7 SP Corydalidae 2 32 I AF BVW PR 5 CR Beetles (Coleoptera) Haliplidae 1 16 A AF BVW MP 8 GN Dysticidae 7 111 I AF BVW PR 6 GN Hydrophilidae 1 16 A AF BVW PR 8 GN Elmidae 3 48 I BVW SC 4 CG True Flies (Diptera) Chironomidae 8 127 I AF BVW CG 6 BU Simuliidae 7 111 I AF BVW CF 6 CG Tipulidae 4 63 I AF BVW SH 4 GU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
gg
hh
Other Total Individuals 63
(70) 1000
Total Taxa 17 (18)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Veliidae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-5 STATION NAME Lick Run LAT N 39deg 12752rsquo (upstream) LONG W 078deg 05685rsquo (upstream) LAT N 39deg 12784rsquo (downstream) LONG W 078deg 05631rsquo (downstream) FORM COMPILED BY Brandon Millholland W Bousquet RIVER BASIN Potomac River INVESTIGATORS J Pangle S Robertson W Bousquet JTovar M Dahl
Collection date 29 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Gomphidae 1 15 I BVW PR 3 BU Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Leptophlebiidae 11 167 A LBM BVW CG 2 CR Plecoptera (stoneflies) Perlidae 10 152 A LBM BVW PR 1 CR Perlodiae 1 15 A LBM BVW PR 2 CR Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 5 76 A LBM BVW SC 4 CG Elmidae 2 30 I LBM BVW SC 4 CG Diptera (true flies) (Culicidae) (3) - P LMB BVW ( ) Chironomidae 23 348 I LBM BVW CG 6 BU Simuliidae 13 197 I LBM BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
ii
jj
Other Total Individuals 66
(69) 1000
Total Taxa 7 (8)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Culicidae (Diptera) pupae not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl J Pangle S Robertson W Bousquet B Millholland J Tovar H Kepple
Collection date 30 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 1 12 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Asellidae 114 965 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Plecoptera (stoneflies) Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 2 23 A MRD BVW SC 4 CG Diptera (true flies)
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
kk
ll
Other Total Individuals 117 1000 Total Taxa 3
Notes Subsample taken 29 October 2009 All taxonomic determinations by MRD were confirmed by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson K McCann A Fisher H Kepple A Barrett
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 2 17 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Sphaeriidae 2 17 I MRD BVW CF 8 BU Isopoda (isopods) Asellidae 99 853 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Calopterygidae 2 17 A MRD BVW PR 7 CL Ephemeroptera (mayflies) Leptophlebiidae 2 17 I MRD BVW CG 2 CR Heptageniidae 4 34 A MRD BVW SC 3 CG Plecoptera (stoneflies) Perlidae 1 09 A MRD BVW PR 1 CR Trichoptera (caddisflies) Hemiptera (true bugs) (Gerridae) (1) - A MRD BVW ( ) Corixidae 1 09 A MRD BVW PR 5 CR Megaloptera (dobsonflies etc) Coleoptera (beetles) Dryopidae 1 09 A MRD BVW SC 5 CG Diptera (true flies) Tipulidae 2 17 I BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
mm
nn
Other Total Individuals 116
(117) 999
Total Taxa 10 (11)
Notes Subsample taken 6 November 2008 Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009 Gerridae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
- Disclaimer ii
- Title Page iii
- Acknowledgements v
- Table of Contents vii
- SECTION 2 RESULTS DATA TABLES 7
- OVERVIEW AND FINDINGS
- Introduction
- RESULTS DATA TABLES
- REFERENCES
- FIELD DATA SHEETS
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- SubstrateType
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- WATER QUALITY ANALYSIS
- THROUGH THE VIRGINIA
- STREAM CONDITION INDEX (VSCI)
- LABORATORY BENCH SHEETS
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
r
s
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-1 STATION NAME Mouth LAT N 39deg 12749rsquo (upstream) LONG W 078deg 04209rsquo (upstream) LAT N 39deg 12729rsquo (downstream) LONG W 078deg 04148rsquo (downstream) FORM COMPILED BY Jeremy Tovar W S Bousquet RIVER BASIN Potomac River INVESTIGATORS Marie Dahl Brandon Millholand J W Pangle Sean Robertson Jeremy Tovar Woodward Bousquet Ben Bousquet
Collection date 21 May 2008 Sheet completion date 12 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Hirudinea 3 33 JT BVW PR 8 SP Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Coenagrionidae 2 22 BVW PR 9 CR Ephemeroptera (mayflies) Caenidae 1 11 JT BVW CG 6 SP Ephemerllidae 4 44 JT BVW CG 2 CR Heptageniidae 5 55 JT BVW SC 3 CG Isonychiidae 1 11 BVW CF 3 CG Plecoptera (stoneflies) Perlidae 14 154 JT BVW PR 2 CR Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Corydalidae 1 11 JT BVW PR 5 CR Coleoptera (beetles) Elmidae 24 264 I JT BVW SC 4 CG Dytiscidae 4 44 JT BVW PR 6 GN Psephenidae 3 33 JT BVW SC 4 CG Diptera (true flies) Tipulidae 3 33 JT BVW SH 4 BU Chironomidae 26 286 JT BVW CG 6 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
t
Other Total Individuals 91 1001 Total Taxa 13
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
u
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-1 STATION NAME Mouth LAT N 39deg 12749rsquo (upstream) LONG W 078deg 04209rsquo (upstream) LAT N 39deg 12729rsquo (downstream) LONG W 078deg 04148rsquo (downstream) FORM COMPILED BY Jeremy Tovar W S Bousquet RIVER BASIN Potomac River INVESTIGATORS Brandon Millholand JW Pangle Jeremy Tovar Heather Kepple
Collection date 10 October 2008 Sheet completion date 12 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Oligochaeta 3 35 JT BVW CG 8 BU Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Coenagrionidae 2 23 JT BVW PR 9 CR Ephemeroptera (mayflies) Heptageniidae 12 140 JT BVW SC 3 CG Isonychiidae 2 23 BVW CF 3 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Hydropsychidae 16 186 JT BVW CF 6 CG Philopotamidae 31 360 JT BVW CF 2 CG Hemiptera (true bugs) (Veliidae) (5) - JT BVW ( ) Megaloptera (dobsonflies etc) Corydalidae 10 116 JT BVW PR 5 CR Coleoptera (beetles) Dytiscidae 1 12 JT BVW PR 6 GN Psephenidae 7 81 JT BVW SC 4 CG Diptera (true flies) Tipulidae 2 23 BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
v
Other Total Individuals 86
(91) 999
Total Taxa 10 (11)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009 Veliidae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
w
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-2 STATION NAME Package Plant LAT N 39deg 12958rsquo (upstream) LONG W 078deg 04900 (upstream) LAT N 39deg 12958rsquo (downstream) LONG W 078deg 04822 (downstream) FORM COMPILED BY JW Pangle W Bousquet RIVER BASIN Potomac River INVESTIGATORS JW Pangle S Robertson J Tovar M Dahl B Millholland W Bousquet
Collection date 29 May 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Plecoptera (stoneflies) Perlidae 11 136 I JP BVW PR 1 CR Trichoptera (caddisflies) Hydropsychidae 10 123 I JP BVW CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 14 173 I JP BVW SC 4 CG Diptera (true flies) Chironomidae 32 395 I JP BVW CG 6 BU Simuliidae 14 173 I JP BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
x
Other Total Individuals 81 1000 Total Taxa 5
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-2 STATION NAME Package Plant LAT N 39deg 12958rsquo (upstream) LONG W 078deg 04900 (upstream) LAT N 39deg 12958rsquo (downstream) LONG W 078deg 04822 (downstream) FORM COMPILED BY JW Pangle W Bousquet RIVER BASIN Potomac River INVESTIGATORS JW Pangle Brandon Millholland Jeremy Tovar Heather Kepple
Collection date 10 October 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Heptageniidae 40 392 I WBJP SC 3 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Hydropsychidae 7 69 I JP WB BVW CF 6 CG Philopotamidae 28 275 I JP WB BVW CF 2 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Corydalidae 6 59 I JP WB BVW PR 5 CR Coleoptera (beetles) Psephenidae 7 69 I JP WB BVW SC 4 CG Elmidae 8 78 I JP WB BVW SC 4 CG Diptera (true flies) Tipulidae 6 59 I JP WB BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
y
z
Other Total Individuals 102 1001 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-3 STATION NAME Rt 664 Bridge LAT N 39deg 12934rsquo (upstream) LONG W 078deg 05019rsquo (upstream) LAT N 39deg 12951rsquo (downstream) LONG W 078deg 04958rsquo (downstream) FORM COMPILED BY S Robertson W Bousquet RIVER BASIN Potomac River INVESTIGATORS SRobertson BMillholland WBousquet MDahl JPangle
Collection date 28 May 2008 Sheet completion date 11 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Leptophlebiidae 1 15 I SGR BVW CG 2 CR Baetidae 1 15 I BVW CG 5 CG Plecoptera (stoneflies) Perlidae 3 46 I SGR BVW PR 1 CR Nemouridae 1 15 I BVW SH 3 CR Trichoptera (caddisflies) Hydropsychidae 5 77 I SGR BVW CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 27 415 I SGR BVW SC 4 CG Psephenidae 2 31 A SGR BVW SC 4 CG Diptera (true flies) Simuliidae 5 77 I SGR BVW CF 6 CG Chironomidae 20 308 I SGR BVW CG 6 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
aa
bb
Other Total Individuals 65 999 Total Taxa 9
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-3 STATION NAME Rt 664 Bridge LAT N 39deg 12934rsquo (upstream) LONG W 078deg 05019rsquo (upstream) LAT N 39deg 12951rsquo (downstream) LONG W 078deg 04958rsquo (downstream) FORM COMPILED BY S Robertson RIVER BASIN Potomac River INVESTIGATORS S Robertson M Dahl A Fisher H Kepple
Collection date 10 Oct 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Heptageniidae 2 20 I SGR SC 3 CG Isonychiidae 2 20 I BVW CF 2 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Philopotamidae 36 367 I SGR CF 2 CG Hydropsychidae 47 480 I SGR CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 2 20 A SGR SC 4 CG Psephenidae 8 82 A SGR SC 4 CG Diptera (true flies) Tabanidae 1 10 I SGR PR 7 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
cc
dd
Other Total Individuals 98 999 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-4 STATION NAME Unnamed Tributary LAT (upstream) N 39deg 13035rsquo LONG (upstream) W 078deg 05129rsquo LAT (downstream) N 39deg 13000rsquo LONG (downstream) W 078deg 5086rsquo FORM COMPILED BY A Fisher M Dahl rev W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson A Fisher H Kepple
Collection date 31 May 2008 Sheet completion date rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Flatworms (Turbellaria) Worms (Oligochaeta) Naididae 1 14 - BVW CG 8 BU Leeches (Hirudinea) Snails (Gastropoda) Clams (Bivalvia) Isopods (Isopoda) Asellidae 3 42 A AF BVW CG 8 SP Scuds (Amphipoda) Crayfish Shrimp (Decapoda) Water Mites (Hydracarina) Dragonflies (Odonata Anisoptera) Damselflies (Odonata Zygoptera) Mayflies (Ephemeroptera) Stoneflies (Plecoptera) Perlidae 2 28 I AF BVW PR 1 CR Caddisflies (Trichoptera) True Bugs (Hemiptera) Corixidae 1 14 I AF BVW MP 9 DV Dobsonflies (Megaloptera) Beetles (Coleoptera) Dytiscidae 1 14 I AF BVW PR 6 GN True Flies (Diptera) Chironomidae 61 847 I AF BVW CG 6 BU Simuliidae 3 42 I AF BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
ee
ff
Other Total Individuals 72 1001 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-4 STATION NAME Unnamed Tributary LAT (upstream) N 39deg 13035rsquo LONG (upstream) W 078deg 05129rsquo LAT (downstream) N 39deg 13000rsquo LONG (downstream) W 078deg 5086rsquo FORM COMPILED BY Andrew G M Fisher W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson A Fisher H Kepple
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Flatworms (Turbellaria) Worms (Oligochaeta) Lumbriculidae 2 32 A AF BVW CG 8 BU Leeches (Hirudinea) Snails (Gastropoda) Lymnaeidae 1 16 A BVW CG 9 GN Clams (Bivalvia) Isopods (Isopoda) Scuds (Amphipoda) Crayfish Shrimp (Decapoda) Cambaridae 1 16 A AF BVW GN 5 GN Water Mites (Hydracarina) Dragonflies (Odonata Anisoptera) Damselflies (Odonata Zygoptera) Calopterygidae 4 63 I BVW PR 7 CL Mayflies (Ephemeroptera) Tricorythidae 1 16 I BVW CG 5 SP Stoneflies (Plecoptera) Caddisflies (Trichoptera) Phryganeidae 4 63 I AF BVW SH 4 CR Hydropsychidae 6 95 I AF BVW CF 6 CG I True Bugs (Hemiptera) Corixidae 8 127 I AF BVW MP 9 DV (Veliidae ) (7) - I AF BVW ( ) Dobsonflies (Megaloptera) Sialidae 3 48 I AF BVW PR 7 SP Corydalidae 2 32 I AF BVW PR 5 CR Beetles (Coleoptera) Haliplidae 1 16 A AF BVW MP 8 GN Dysticidae 7 111 I AF BVW PR 6 GN Hydrophilidae 1 16 A AF BVW PR 8 GN Elmidae 3 48 I BVW SC 4 CG True Flies (Diptera) Chironomidae 8 127 I AF BVW CG 6 BU Simuliidae 7 111 I AF BVW CF 6 CG Tipulidae 4 63 I AF BVW SH 4 GU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
gg
hh
Other Total Individuals 63
(70) 1000
Total Taxa 17 (18)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Veliidae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-5 STATION NAME Lick Run LAT N 39deg 12752rsquo (upstream) LONG W 078deg 05685rsquo (upstream) LAT N 39deg 12784rsquo (downstream) LONG W 078deg 05631rsquo (downstream) FORM COMPILED BY Brandon Millholland W Bousquet RIVER BASIN Potomac River INVESTIGATORS J Pangle S Robertson W Bousquet JTovar M Dahl
Collection date 29 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Gomphidae 1 15 I BVW PR 3 BU Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Leptophlebiidae 11 167 A LBM BVW CG 2 CR Plecoptera (stoneflies) Perlidae 10 152 A LBM BVW PR 1 CR Perlodiae 1 15 A LBM BVW PR 2 CR Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 5 76 A LBM BVW SC 4 CG Elmidae 2 30 I LBM BVW SC 4 CG Diptera (true flies) (Culicidae) (3) - P LMB BVW ( ) Chironomidae 23 348 I LBM BVW CG 6 BU Simuliidae 13 197 I LBM BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
ii
jj
Other Total Individuals 66
(69) 1000
Total Taxa 7 (8)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Culicidae (Diptera) pupae not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl J Pangle S Robertson W Bousquet B Millholland J Tovar H Kepple
Collection date 30 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 1 12 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Asellidae 114 965 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Plecoptera (stoneflies) Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 2 23 A MRD BVW SC 4 CG Diptera (true flies)
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
kk
ll
Other Total Individuals 117 1000 Total Taxa 3
Notes Subsample taken 29 October 2009 All taxonomic determinations by MRD were confirmed by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson K McCann A Fisher H Kepple A Barrett
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 2 17 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Sphaeriidae 2 17 I MRD BVW CF 8 BU Isopoda (isopods) Asellidae 99 853 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Calopterygidae 2 17 A MRD BVW PR 7 CL Ephemeroptera (mayflies) Leptophlebiidae 2 17 I MRD BVW CG 2 CR Heptageniidae 4 34 A MRD BVW SC 3 CG Plecoptera (stoneflies) Perlidae 1 09 A MRD BVW PR 1 CR Trichoptera (caddisflies) Hemiptera (true bugs) (Gerridae) (1) - A MRD BVW ( ) Corixidae 1 09 A MRD BVW PR 5 CR Megaloptera (dobsonflies etc) Coleoptera (beetles) Dryopidae 1 09 A MRD BVW SC 5 CG Diptera (true flies) Tipulidae 2 17 I BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
mm
nn
Other Total Individuals 116
(117) 999
Total Taxa 10 (11)
Notes Subsample taken 6 November 2008 Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009 Gerridae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
- Disclaimer ii
- Title Page iii
- Acknowledgements v
- Table of Contents vii
- SECTION 2 RESULTS DATA TABLES 7
- OVERVIEW AND FINDINGS
- Introduction
- RESULTS DATA TABLES
- REFERENCES
- FIELD DATA SHEETS
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- SubstrateType
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- WATER QUALITY ANALYSIS
- THROUGH THE VIRGINIA
- STREAM CONDITION INDEX (VSCI)
- LABORATORY BENCH SHEETS
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
s
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-1 STATION NAME Mouth LAT N 39deg 12749rsquo (upstream) LONG W 078deg 04209rsquo (upstream) LAT N 39deg 12729rsquo (downstream) LONG W 078deg 04148rsquo (downstream) FORM COMPILED BY Jeremy Tovar W S Bousquet RIVER BASIN Potomac River INVESTIGATORS Marie Dahl Brandon Millholand J W Pangle Sean Robertson Jeremy Tovar Woodward Bousquet Ben Bousquet
Collection date 21 May 2008 Sheet completion date 12 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Hirudinea 3 33 JT BVW PR 8 SP Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Coenagrionidae 2 22 BVW PR 9 CR Ephemeroptera (mayflies) Caenidae 1 11 JT BVW CG 6 SP Ephemerllidae 4 44 JT BVW CG 2 CR Heptageniidae 5 55 JT BVW SC 3 CG Isonychiidae 1 11 BVW CF 3 CG Plecoptera (stoneflies) Perlidae 14 154 JT BVW PR 2 CR Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Corydalidae 1 11 JT BVW PR 5 CR Coleoptera (beetles) Elmidae 24 264 I JT BVW SC 4 CG Dytiscidae 4 44 JT BVW PR 6 GN Psephenidae 3 33 JT BVW SC 4 CG Diptera (true flies) Tipulidae 3 33 JT BVW SH 4 BU Chironomidae 26 286 JT BVW CG 6 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
t
Other Total Individuals 91 1001 Total Taxa 13
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
u
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-1 STATION NAME Mouth LAT N 39deg 12749rsquo (upstream) LONG W 078deg 04209rsquo (upstream) LAT N 39deg 12729rsquo (downstream) LONG W 078deg 04148rsquo (downstream) FORM COMPILED BY Jeremy Tovar W S Bousquet RIVER BASIN Potomac River INVESTIGATORS Brandon Millholand JW Pangle Jeremy Tovar Heather Kepple
Collection date 10 October 2008 Sheet completion date 12 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Oligochaeta 3 35 JT BVW CG 8 BU Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Coenagrionidae 2 23 JT BVW PR 9 CR Ephemeroptera (mayflies) Heptageniidae 12 140 JT BVW SC 3 CG Isonychiidae 2 23 BVW CF 3 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Hydropsychidae 16 186 JT BVW CF 6 CG Philopotamidae 31 360 JT BVW CF 2 CG Hemiptera (true bugs) (Veliidae) (5) - JT BVW ( ) Megaloptera (dobsonflies etc) Corydalidae 10 116 JT BVW PR 5 CR Coleoptera (beetles) Dytiscidae 1 12 JT BVW PR 6 GN Psephenidae 7 81 JT BVW SC 4 CG Diptera (true flies) Tipulidae 2 23 BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
v
Other Total Individuals 86
(91) 999
Total Taxa 10 (11)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009 Veliidae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
w
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-2 STATION NAME Package Plant LAT N 39deg 12958rsquo (upstream) LONG W 078deg 04900 (upstream) LAT N 39deg 12958rsquo (downstream) LONG W 078deg 04822 (downstream) FORM COMPILED BY JW Pangle W Bousquet RIVER BASIN Potomac River INVESTIGATORS JW Pangle S Robertson J Tovar M Dahl B Millholland W Bousquet
Collection date 29 May 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Plecoptera (stoneflies) Perlidae 11 136 I JP BVW PR 1 CR Trichoptera (caddisflies) Hydropsychidae 10 123 I JP BVW CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 14 173 I JP BVW SC 4 CG Diptera (true flies) Chironomidae 32 395 I JP BVW CG 6 BU Simuliidae 14 173 I JP BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
x
Other Total Individuals 81 1000 Total Taxa 5
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-2 STATION NAME Package Plant LAT N 39deg 12958rsquo (upstream) LONG W 078deg 04900 (upstream) LAT N 39deg 12958rsquo (downstream) LONG W 078deg 04822 (downstream) FORM COMPILED BY JW Pangle W Bousquet RIVER BASIN Potomac River INVESTIGATORS JW Pangle Brandon Millholland Jeremy Tovar Heather Kepple
Collection date 10 October 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Heptageniidae 40 392 I WBJP SC 3 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Hydropsychidae 7 69 I JP WB BVW CF 6 CG Philopotamidae 28 275 I JP WB BVW CF 2 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Corydalidae 6 59 I JP WB BVW PR 5 CR Coleoptera (beetles) Psephenidae 7 69 I JP WB BVW SC 4 CG Elmidae 8 78 I JP WB BVW SC 4 CG Diptera (true flies) Tipulidae 6 59 I JP WB BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
y
z
Other Total Individuals 102 1001 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-3 STATION NAME Rt 664 Bridge LAT N 39deg 12934rsquo (upstream) LONG W 078deg 05019rsquo (upstream) LAT N 39deg 12951rsquo (downstream) LONG W 078deg 04958rsquo (downstream) FORM COMPILED BY S Robertson W Bousquet RIVER BASIN Potomac River INVESTIGATORS SRobertson BMillholland WBousquet MDahl JPangle
Collection date 28 May 2008 Sheet completion date 11 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Leptophlebiidae 1 15 I SGR BVW CG 2 CR Baetidae 1 15 I BVW CG 5 CG Plecoptera (stoneflies) Perlidae 3 46 I SGR BVW PR 1 CR Nemouridae 1 15 I BVW SH 3 CR Trichoptera (caddisflies) Hydropsychidae 5 77 I SGR BVW CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 27 415 I SGR BVW SC 4 CG Psephenidae 2 31 A SGR BVW SC 4 CG Diptera (true flies) Simuliidae 5 77 I SGR BVW CF 6 CG Chironomidae 20 308 I SGR BVW CG 6 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
aa
bb
Other Total Individuals 65 999 Total Taxa 9
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-3 STATION NAME Rt 664 Bridge LAT N 39deg 12934rsquo (upstream) LONG W 078deg 05019rsquo (upstream) LAT N 39deg 12951rsquo (downstream) LONG W 078deg 04958rsquo (downstream) FORM COMPILED BY S Robertson RIVER BASIN Potomac River INVESTIGATORS S Robertson M Dahl A Fisher H Kepple
Collection date 10 Oct 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Heptageniidae 2 20 I SGR SC 3 CG Isonychiidae 2 20 I BVW CF 2 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Philopotamidae 36 367 I SGR CF 2 CG Hydropsychidae 47 480 I SGR CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 2 20 A SGR SC 4 CG Psephenidae 8 82 A SGR SC 4 CG Diptera (true flies) Tabanidae 1 10 I SGR PR 7 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
cc
dd
Other Total Individuals 98 999 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-4 STATION NAME Unnamed Tributary LAT (upstream) N 39deg 13035rsquo LONG (upstream) W 078deg 05129rsquo LAT (downstream) N 39deg 13000rsquo LONG (downstream) W 078deg 5086rsquo FORM COMPILED BY A Fisher M Dahl rev W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson A Fisher H Kepple
Collection date 31 May 2008 Sheet completion date rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Flatworms (Turbellaria) Worms (Oligochaeta) Naididae 1 14 - BVW CG 8 BU Leeches (Hirudinea) Snails (Gastropoda) Clams (Bivalvia) Isopods (Isopoda) Asellidae 3 42 A AF BVW CG 8 SP Scuds (Amphipoda) Crayfish Shrimp (Decapoda) Water Mites (Hydracarina) Dragonflies (Odonata Anisoptera) Damselflies (Odonata Zygoptera) Mayflies (Ephemeroptera) Stoneflies (Plecoptera) Perlidae 2 28 I AF BVW PR 1 CR Caddisflies (Trichoptera) True Bugs (Hemiptera) Corixidae 1 14 I AF BVW MP 9 DV Dobsonflies (Megaloptera) Beetles (Coleoptera) Dytiscidae 1 14 I AF BVW PR 6 GN True Flies (Diptera) Chironomidae 61 847 I AF BVW CG 6 BU Simuliidae 3 42 I AF BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
ee
ff
Other Total Individuals 72 1001 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-4 STATION NAME Unnamed Tributary LAT (upstream) N 39deg 13035rsquo LONG (upstream) W 078deg 05129rsquo LAT (downstream) N 39deg 13000rsquo LONG (downstream) W 078deg 5086rsquo FORM COMPILED BY Andrew G M Fisher W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson A Fisher H Kepple
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Flatworms (Turbellaria) Worms (Oligochaeta) Lumbriculidae 2 32 A AF BVW CG 8 BU Leeches (Hirudinea) Snails (Gastropoda) Lymnaeidae 1 16 A BVW CG 9 GN Clams (Bivalvia) Isopods (Isopoda) Scuds (Amphipoda) Crayfish Shrimp (Decapoda) Cambaridae 1 16 A AF BVW GN 5 GN Water Mites (Hydracarina) Dragonflies (Odonata Anisoptera) Damselflies (Odonata Zygoptera) Calopterygidae 4 63 I BVW PR 7 CL Mayflies (Ephemeroptera) Tricorythidae 1 16 I BVW CG 5 SP Stoneflies (Plecoptera) Caddisflies (Trichoptera) Phryganeidae 4 63 I AF BVW SH 4 CR Hydropsychidae 6 95 I AF BVW CF 6 CG I True Bugs (Hemiptera) Corixidae 8 127 I AF BVW MP 9 DV (Veliidae ) (7) - I AF BVW ( ) Dobsonflies (Megaloptera) Sialidae 3 48 I AF BVW PR 7 SP Corydalidae 2 32 I AF BVW PR 5 CR Beetles (Coleoptera) Haliplidae 1 16 A AF BVW MP 8 GN Dysticidae 7 111 I AF BVW PR 6 GN Hydrophilidae 1 16 A AF BVW PR 8 GN Elmidae 3 48 I BVW SC 4 CG True Flies (Diptera) Chironomidae 8 127 I AF BVW CG 6 BU Simuliidae 7 111 I AF BVW CF 6 CG Tipulidae 4 63 I AF BVW SH 4 GU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
gg
hh
Other Total Individuals 63
(70) 1000
Total Taxa 17 (18)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Veliidae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-5 STATION NAME Lick Run LAT N 39deg 12752rsquo (upstream) LONG W 078deg 05685rsquo (upstream) LAT N 39deg 12784rsquo (downstream) LONG W 078deg 05631rsquo (downstream) FORM COMPILED BY Brandon Millholland W Bousquet RIVER BASIN Potomac River INVESTIGATORS J Pangle S Robertson W Bousquet JTovar M Dahl
Collection date 29 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Gomphidae 1 15 I BVW PR 3 BU Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Leptophlebiidae 11 167 A LBM BVW CG 2 CR Plecoptera (stoneflies) Perlidae 10 152 A LBM BVW PR 1 CR Perlodiae 1 15 A LBM BVW PR 2 CR Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 5 76 A LBM BVW SC 4 CG Elmidae 2 30 I LBM BVW SC 4 CG Diptera (true flies) (Culicidae) (3) - P LMB BVW ( ) Chironomidae 23 348 I LBM BVW CG 6 BU Simuliidae 13 197 I LBM BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
ii
jj
Other Total Individuals 66
(69) 1000
Total Taxa 7 (8)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Culicidae (Diptera) pupae not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl J Pangle S Robertson W Bousquet B Millholland J Tovar H Kepple
Collection date 30 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 1 12 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Asellidae 114 965 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Plecoptera (stoneflies) Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 2 23 A MRD BVW SC 4 CG Diptera (true flies)
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
kk
ll
Other Total Individuals 117 1000 Total Taxa 3
Notes Subsample taken 29 October 2009 All taxonomic determinations by MRD were confirmed by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson K McCann A Fisher H Kepple A Barrett
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 2 17 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Sphaeriidae 2 17 I MRD BVW CF 8 BU Isopoda (isopods) Asellidae 99 853 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Calopterygidae 2 17 A MRD BVW PR 7 CL Ephemeroptera (mayflies) Leptophlebiidae 2 17 I MRD BVW CG 2 CR Heptageniidae 4 34 A MRD BVW SC 3 CG Plecoptera (stoneflies) Perlidae 1 09 A MRD BVW PR 1 CR Trichoptera (caddisflies) Hemiptera (true bugs) (Gerridae) (1) - A MRD BVW ( ) Corixidae 1 09 A MRD BVW PR 5 CR Megaloptera (dobsonflies etc) Coleoptera (beetles) Dryopidae 1 09 A MRD BVW SC 5 CG Diptera (true flies) Tipulidae 2 17 I BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
mm
nn
Other Total Individuals 116
(117) 999
Total Taxa 10 (11)
Notes Subsample taken 6 November 2008 Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009 Gerridae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
- Disclaimer ii
- Title Page iii
- Acknowledgements v
- Table of Contents vii
- SECTION 2 RESULTS DATA TABLES 7
- OVERVIEW AND FINDINGS
- Introduction
- RESULTS DATA TABLES
- REFERENCES
- FIELD DATA SHEETS
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- SubstrateType
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- WATER QUALITY ANALYSIS
- THROUGH THE VIRGINIA
- STREAM CONDITION INDEX (VSCI)
- LABORATORY BENCH SHEETS
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
t
Other Total Individuals 91 1001 Total Taxa 13
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
u
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-1 STATION NAME Mouth LAT N 39deg 12749rsquo (upstream) LONG W 078deg 04209rsquo (upstream) LAT N 39deg 12729rsquo (downstream) LONG W 078deg 04148rsquo (downstream) FORM COMPILED BY Jeremy Tovar W S Bousquet RIVER BASIN Potomac River INVESTIGATORS Brandon Millholand JW Pangle Jeremy Tovar Heather Kepple
Collection date 10 October 2008 Sheet completion date 12 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Oligochaeta 3 35 JT BVW CG 8 BU Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Coenagrionidae 2 23 JT BVW PR 9 CR Ephemeroptera (mayflies) Heptageniidae 12 140 JT BVW SC 3 CG Isonychiidae 2 23 BVW CF 3 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Hydropsychidae 16 186 JT BVW CF 6 CG Philopotamidae 31 360 JT BVW CF 2 CG Hemiptera (true bugs) (Veliidae) (5) - JT BVW ( ) Megaloptera (dobsonflies etc) Corydalidae 10 116 JT BVW PR 5 CR Coleoptera (beetles) Dytiscidae 1 12 JT BVW PR 6 GN Psephenidae 7 81 JT BVW SC 4 CG Diptera (true flies) Tipulidae 2 23 BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
v
Other Total Individuals 86
(91) 999
Total Taxa 10 (11)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009 Veliidae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
w
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-2 STATION NAME Package Plant LAT N 39deg 12958rsquo (upstream) LONG W 078deg 04900 (upstream) LAT N 39deg 12958rsquo (downstream) LONG W 078deg 04822 (downstream) FORM COMPILED BY JW Pangle W Bousquet RIVER BASIN Potomac River INVESTIGATORS JW Pangle S Robertson J Tovar M Dahl B Millholland W Bousquet
Collection date 29 May 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Plecoptera (stoneflies) Perlidae 11 136 I JP BVW PR 1 CR Trichoptera (caddisflies) Hydropsychidae 10 123 I JP BVW CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 14 173 I JP BVW SC 4 CG Diptera (true flies) Chironomidae 32 395 I JP BVW CG 6 BU Simuliidae 14 173 I JP BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
x
Other Total Individuals 81 1000 Total Taxa 5
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-2 STATION NAME Package Plant LAT N 39deg 12958rsquo (upstream) LONG W 078deg 04900 (upstream) LAT N 39deg 12958rsquo (downstream) LONG W 078deg 04822 (downstream) FORM COMPILED BY JW Pangle W Bousquet RIVER BASIN Potomac River INVESTIGATORS JW Pangle Brandon Millholland Jeremy Tovar Heather Kepple
Collection date 10 October 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Heptageniidae 40 392 I WBJP SC 3 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Hydropsychidae 7 69 I JP WB BVW CF 6 CG Philopotamidae 28 275 I JP WB BVW CF 2 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Corydalidae 6 59 I JP WB BVW PR 5 CR Coleoptera (beetles) Psephenidae 7 69 I JP WB BVW SC 4 CG Elmidae 8 78 I JP WB BVW SC 4 CG Diptera (true flies) Tipulidae 6 59 I JP WB BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
y
z
Other Total Individuals 102 1001 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-3 STATION NAME Rt 664 Bridge LAT N 39deg 12934rsquo (upstream) LONG W 078deg 05019rsquo (upstream) LAT N 39deg 12951rsquo (downstream) LONG W 078deg 04958rsquo (downstream) FORM COMPILED BY S Robertson W Bousquet RIVER BASIN Potomac River INVESTIGATORS SRobertson BMillholland WBousquet MDahl JPangle
Collection date 28 May 2008 Sheet completion date 11 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Leptophlebiidae 1 15 I SGR BVW CG 2 CR Baetidae 1 15 I BVW CG 5 CG Plecoptera (stoneflies) Perlidae 3 46 I SGR BVW PR 1 CR Nemouridae 1 15 I BVW SH 3 CR Trichoptera (caddisflies) Hydropsychidae 5 77 I SGR BVW CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 27 415 I SGR BVW SC 4 CG Psephenidae 2 31 A SGR BVW SC 4 CG Diptera (true flies) Simuliidae 5 77 I SGR BVW CF 6 CG Chironomidae 20 308 I SGR BVW CG 6 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
aa
bb
Other Total Individuals 65 999 Total Taxa 9
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-3 STATION NAME Rt 664 Bridge LAT N 39deg 12934rsquo (upstream) LONG W 078deg 05019rsquo (upstream) LAT N 39deg 12951rsquo (downstream) LONG W 078deg 04958rsquo (downstream) FORM COMPILED BY S Robertson RIVER BASIN Potomac River INVESTIGATORS S Robertson M Dahl A Fisher H Kepple
Collection date 10 Oct 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Heptageniidae 2 20 I SGR SC 3 CG Isonychiidae 2 20 I BVW CF 2 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Philopotamidae 36 367 I SGR CF 2 CG Hydropsychidae 47 480 I SGR CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 2 20 A SGR SC 4 CG Psephenidae 8 82 A SGR SC 4 CG Diptera (true flies) Tabanidae 1 10 I SGR PR 7 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
cc
dd
Other Total Individuals 98 999 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-4 STATION NAME Unnamed Tributary LAT (upstream) N 39deg 13035rsquo LONG (upstream) W 078deg 05129rsquo LAT (downstream) N 39deg 13000rsquo LONG (downstream) W 078deg 5086rsquo FORM COMPILED BY A Fisher M Dahl rev W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson A Fisher H Kepple
Collection date 31 May 2008 Sheet completion date rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Flatworms (Turbellaria) Worms (Oligochaeta) Naididae 1 14 - BVW CG 8 BU Leeches (Hirudinea) Snails (Gastropoda) Clams (Bivalvia) Isopods (Isopoda) Asellidae 3 42 A AF BVW CG 8 SP Scuds (Amphipoda) Crayfish Shrimp (Decapoda) Water Mites (Hydracarina) Dragonflies (Odonata Anisoptera) Damselflies (Odonata Zygoptera) Mayflies (Ephemeroptera) Stoneflies (Plecoptera) Perlidae 2 28 I AF BVW PR 1 CR Caddisflies (Trichoptera) True Bugs (Hemiptera) Corixidae 1 14 I AF BVW MP 9 DV Dobsonflies (Megaloptera) Beetles (Coleoptera) Dytiscidae 1 14 I AF BVW PR 6 GN True Flies (Diptera) Chironomidae 61 847 I AF BVW CG 6 BU Simuliidae 3 42 I AF BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
ee
ff
Other Total Individuals 72 1001 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-4 STATION NAME Unnamed Tributary LAT (upstream) N 39deg 13035rsquo LONG (upstream) W 078deg 05129rsquo LAT (downstream) N 39deg 13000rsquo LONG (downstream) W 078deg 5086rsquo FORM COMPILED BY Andrew G M Fisher W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson A Fisher H Kepple
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Flatworms (Turbellaria) Worms (Oligochaeta) Lumbriculidae 2 32 A AF BVW CG 8 BU Leeches (Hirudinea) Snails (Gastropoda) Lymnaeidae 1 16 A BVW CG 9 GN Clams (Bivalvia) Isopods (Isopoda) Scuds (Amphipoda) Crayfish Shrimp (Decapoda) Cambaridae 1 16 A AF BVW GN 5 GN Water Mites (Hydracarina) Dragonflies (Odonata Anisoptera) Damselflies (Odonata Zygoptera) Calopterygidae 4 63 I BVW PR 7 CL Mayflies (Ephemeroptera) Tricorythidae 1 16 I BVW CG 5 SP Stoneflies (Plecoptera) Caddisflies (Trichoptera) Phryganeidae 4 63 I AF BVW SH 4 CR Hydropsychidae 6 95 I AF BVW CF 6 CG I True Bugs (Hemiptera) Corixidae 8 127 I AF BVW MP 9 DV (Veliidae ) (7) - I AF BVW ( ) Dobsonflies (Megaloptera) Sialidae 3 48 I AF BVW PR 7 SP Corydalidae 2 32 I AF BVW PR 5 CR Beetles (Coleoptera) Haliplidae 1 16 A AF BVW MP 8 GN Dysticidae 7 111 I AF BVW PR 6 GN Hydrophilidae 1 16 A AF BVW PR 8 GN Elmidae 3 48 I BVW SC 4 CG True Flies (Diptera) Chironomidae 8 127 I AF BVW CG 6 BU Simuliidae 7 111 I AF BVW CF 6 CG Tipulidae 4 63 I AF BVW SH 4 GU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
gg
hh
Other Total Individuals 63
(70) 1000
Total Taxa 17 (18)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Veliidae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-5 STATION NAME Lick Run LAT N 39deg 12752rsquo (upstream) LONG W 078deg 05685rsquo (upstream) LAT N 39deg 12784rsquo (downstream) LONG W 078deg 05631rsquo (downstream) FORM COMPILED BY Brandon Millholland W Bousquet RIVER BASIN Potomac River INVESTIGATORS J Pangle S Robertson W Bousquet JTovar M Dahl
Collection date 29 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Gomphidae 1 15 I BVW PR 3 BU Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Leptophlebiidae 11 167 A LBM BVW CG 2 CR Plecoptera (stoneflies) Perlidae 10 152 A LBM BVW PR 1 CR Perlodiae 1 15 A LBM BVW PR 2 CR Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 5 76 A LBM BVW SC 4 CG Elmidae 2 30 I LBM BVW SC 4 CG Diptera (true flies) (Culicidae) (3) - P LMB BVW ( ) Chironomidae 23 348 I LBM BVW CG 6 BU Simuliidae 13 197 I LBM BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
ii
jj
Other Total Individuals 66
(69) 1000
Total Taxa 7 (8)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Culicidae (Diptera) pupae not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl J Pangle S Robertson W Bousquet B Millholland J Tovar H Kepple
Collection date 30 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 1 12 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Asellidae 114 965 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Plecoptera (stoneflies) Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 2 23 A MRD BVW SC 4 CG Diptera (true flies)
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
kk
ll
Other Total Individuals 117 1000 Total Taxa 3
Notes Subsample taken 29 October 2009 All taxonomic determinations by MRD were confirmed by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson K McCann A Fisher H Kepple A Barrett
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 2 17 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Sphaeriidae 2 17 I MRD BVW CF 8 BU Isopoda (isopods) Asellidae 99 853 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Calopterygidae 2 17 A MRD BVW PR 7 CL Ephemeroptera (mayflies) Leptophlebiidae 2 17 I MRD BVW CG 2 CR Heptageniidae 4 34 A MRD BVW SC 3 CG Plecoptera (stoneflies) Perlidae 1 09 A MRD BVW PR 1 CR Trichoptera (caddisflies) Hemiptera (true bugs) (Gerridae) (1) - A MRD BVW ( ) Corixidae 1 09 A MRD BVW PR 5 CR Megaloptera (dobsonflies etc) Coleoptera (beetles) Dryopidae 1 09 A MRD BVW SC 5 CG Diptera (true flies) Tipulidae 2 17 I BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
mm
nn
Other Total Individuals 116
(117) 999
Total Taxa 10 (11)
Notes Subsample taken 6 November 2008 Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009 Gerridae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
- Disclaimer ii
- Title Page iii
- Acknowledgements v
- Table of Contents vii
- SECTION 2 RESULTS DATA TABLES 7
- OVERVIEW AND FINDINGS
- Introduction
- RESULTS DATA TABLES
- REFERENCES
- FIELD DATA SHEETS
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- SubstrateType
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- WATER QUALITY ANALYSIS
- THROUGH THE VIRGINIA
- STREAM CONDITION INDEX (VSCI)
- LABORATORY BENCH SHEETS
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
u
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-1 STATION NAME Mouth LAT N 39deg 12749rsquo (upstream) LONG W 078deg 04209rsquo (upstream) LAT N 39deg 12729rsquo (downstream) LONG W 078deg 04148rsquo (downstream) FORM COMPILED BY Jeremy Tovar W S Bousquet RIVER BASIN Potomac River INVESTIGATORS Brandon Millholand JW Pangle Jeremy Tovar Heather Kepple
Collection date 10 October 2008 Sheet completion date 12 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Oligochaeta 3 35 JT BVW CG 8 BU Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Coenagrionidae 2 23 JT BVW PR 9 CR Ephemeroptera (mayflies) Heptageniidae 12 140 JT BVW SC 3 CG Isonychiidae 2 23 BVW CF 3 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Hydropsychidae 16 186 JT BVW CF 6 CG Philopotamidae 31 360 JT BVW CF 2 CG Hemiptera (true bugs) (Veliidae) (5) - JT BVW ( ) Megaloptera (dobsonflies etc) Corydalidae 10 116 JT BVW PR 5 CR Coleoptera (beetles) Dytiscidae 1 12 JT BVW PR 6 GN Psephenidae 7 81 JT BVW SC 4 CG Diptera (true flies) Tipulidae 2 23 BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
v
Other Total Individuals 86
(91) 999
Total Taxa 10 (11)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009 Veliidae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
w
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-2 STATION NAME Package Plant LAT N 39deg 12958rsquo (upstream) LONG W 078deg 04900 (upstream) LAT N 39deg 12958rsquo (downstream) LONG W 078deg 04822 (downstream) FORM COMPILED BY JW Pangle W Bousquet RIVER BASIN Potomac River INVESTIGATORS JW Pangle S Robertson J Tovar M Dahl B Millholland W Bousquet
Collection date 29 May 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Plecoptera (stoneflies) Perlidae 11 136 I JP BVW PR 1 CR Trichoptera (caddisflies) Hydropsychidae 10 123 I JP BVW CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 14 173 I JP BVW SC 4 CG Diptera (true flies) Chironomidae 32 395 I JP BVW CG 6 BU Simuliidae 14 173 I JP BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
x
Other Total Individuals 81 1000 Total Taxa 5
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-2 STATION NAME Package Plant LAT N 39deg 12958rsquo (upstream) LONG W 078deg 04900 (upstream) LAT N 39deg 12958rsquo (downstream) LONG W 078deg 04822 (downstream) FORM COMPILED BY JW Pangle W Bousquet RIVER BASIN Potomac River INVESTIGATORS JW Pangle Brandon Millholland Jeremy Tovar Heather Kepple
Collection date 10 October 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Heptageniidae 40 392 I WBJP SC 3 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Hydropsychidae 7 69 I JP WB BVW CF 6 CG Philopotamidae 28 275 I JP WB BVW CF 2 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Corydalidae 6 59 I JP WB BVW PR 5 CR Coleoptera (beetles) Psephenidae 7 69 I JP WB BVW SC 4 CG Elmidae 8 78 I JP WB BVW SC 4 CG Diptera (true flies) Tipulidae 6 59 I JP WB BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
y
z
Other Total Individuals 102 1001 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-3 STATION NAME Rt 664 Bridge LAT N 39deg 12934rsquo (upstream) LONG W 078deg 05019rsquo (upstream) LAT N 39deg 12951rsquo (downstream) LONG W 078deg 04958rsquo (downstream) FORM COMPILED BY S Robertson W Bousquet RIVER BASIN Potomac River INVESTIGATORS SRobertson BMillholland WBousquet MDahl JPangle
Collection date 28 May 2008 Sheet completion date 11 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Leptophlebiidae 1 15 I SGR BVW CG 2 CR Baetidae 1 15 I BVW CG 5 CG Plecoptera (stoneflies) Perlidae 3 46 I SGR BVW PR 1 CR Nemouridae 1 15 I BVW SH 3 CR Trichoptera (caddisflies) Hydropsychidae 5 77 I SGR BVW CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 27 415 I SGR BVW SC 4 CG Psephenidae 2 31 A SGR BVW SC 4 CG Diptera (true flies) Simuliidae 5 77 I SGR BVW CF 6 CG Chironomidae 20 308 I SGR BVW CG 6 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
aa
bb
Other Total Individuals 65 999 Total Taxa 9
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-3 STATION NAME Rt 664 Bridge LAT N 39deg 12934rsquo (upstream) LONG W 078deg 05019rsquo (upstream) LAT N 39deg 12951rsquo (downstream) LONG W 078deg 04958rsquo (downstream) FORM COMPILED BY S Robertson RIVER BASIN Potomac River INVESTIGATORS S Robertson M Dahl A Fisher H Kepple
Collection date 10 Oct 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Heptageniidae 2 20 I SGR SC 3 CG Isonychiidae 2 20 I BVW CF 2 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Philopotamidae 36 367 I SGR CF 2 CG Hydropsychidae 47 480 I SGR CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 2 20 A SGR SC 4 CG Psephenidae 8 82 A SGR SC 4 CG Diptera (true flies) Tabanidae 1 10 I SGR PR 7 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
cc
dd
Other Total Individuals 98 999 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-4 STATION NAME Unnamed Tributary LAT (upstream) N 39deg 13035rsquo LONG (upstream) W 078deg 05129rsquo LAT (downstream) N 39deg 13000rsquo LONG (downstream) W 078deg 5086rsquo FORM COMPILED BY A Fisher M Dahl rev W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson A Fisher H Kepple
Collection date 31 May 2008 Sheet completion date rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Flatworms (Turbellaria) Worms (Oligochaeta) Naididae 1 14 - BVW CG 8 BU Leeches (Hirudinea) Snails (Gastropoda) Clams (Bivalvia) Isopods (Isopoda) Asellidae 3 42 A AF BVW CG 8 SP Scuds (Amphipoda) Crayfish Shrimp (Decapoda) Water Mites (Hydracarina) Dragonflies (Odonata Anisoptera) Damselflies (Odonata Zygoptera) Mayflies (Ephemeroptera) Stoneflies (Plecoptera) Perlidae 2 28 I AF BVW PR 1 CR Caddisflies (Trichoptera) True Bugs (Hemiptera) Corixidae 1 14 I AF BVW MP 9 DV Dobsonflies (Megaloptera) Beetles (Coleoptera) Dytiscidae 1 14 I AF BVW PR 6 GN True Flies (Diptera) Chironomidae 61 847 I AF BVW CG 6 BU Simuliidae 3 42 I AF BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
ee
ff
Other Total Individuals 72 1001 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-4 STATION NAME Unnamed Tributary LAT (upstream) N 39deg 13035rsquo LONG (upstream) W 078deg 05129rsquo LAT (downstream) N 39deg 13000rsquo LONG (downstream) W 078deg 5086rsquo FORM COMPILED BY Andrew G M Fisher W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson A Fisher H Kepple
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Flatworms (Turbellaria) Worms (Oligochaeta) Lumbriculidae 2 32 A AF BVW CG 8 BU Leeches (Hirudinea) Snails (Gastropoda) Lymnaeidae 1 16 A BVW CG 9 GN Clams (Bivalvia) Isopods (Isopoda) Scuds (Amphipoda) Crayfish Shrimp (Decapoda) Cambaridae 1 16 A AF BVW GN 5 GN Water Mites (Hydracarina) Dragonflies (Odonata Anisoptera) Damselflies (Odonata Zygoptera) Calopterygidae 4 63 I BVW PR 7 CL Mayflies (Ephemeroptera) Tricorythidae 1 16 I BVW CG 5 SP Stoneflies (Plecoptera) Caddisflies (Trichoptera) Phryganeidae 4 63 I AF BVW SH 4 CR Hydropsychidae 6 95 I AF BVW CF 6 CG I True Bugs (Hemiptera) Corixidae 8 127 I AF BVW MP 9 DV (Veliidae ) (7) - I AF BVW ( ) Dobsonflies (Megaloptera) Sialidae 3 48 I AF BVW PR 7 SP Corydalidae 2 32 I AF BVW PR 5 CR Beetles (Coleoptera) Haliplidae 1 16 A AF BVW MP 8 GN Dysticidae 7 111 I AF BVW PR 6 GN Hydrophilidae 1 16 A AF BVW PR 8 GN Elmidae 3 48 I BVW SC 4 CG True Flies (Diptera) Chironomidae 8 127 I AF BVW CG 6 BU Simuliidae 7 111 I AF BVW CF 6 CG Tipulidae 4 63 I AF BVW SH 4 GU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
gg
hh
Other Total Individuals 63
(70) 1000
Total Taxa 17 (18)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Veliidae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-5 STATION NAME Lick Run LAT N 39deg 12752rsquo (upstream) LONG W 078deg 05685rsquo (upstream) LAT N 39deg 12784rsquo (downstream) LONG W 078deg 05631rsquo (downstream) FORM COMPILED BY Brandon Millholland W Bousquet RIVER BASIN Potomac River INVESTIGATORS J Pangle S Robertson W Bousquet JTovar M Dahl
Collection date 29 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Gomphidae 1 15 I BVW PR 3 BU Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Leptophlebiidae 11 167 A LBM BVW CG 2 CR Plecoptera (stoneflies) Perlidae 10 152 A LBM BVW PR 1 CR Perlodiae 1 15 A LBM BVW PR 2 CR Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 5 76 A LBM BVW SC 4 CG Elmidae 2 30 I LBM BVW SC 4 CG Diptera (true flies) (Culicidae) (3) - P LMB BVW ( ) Chironomidae 23 348 I LBM BVW CG 6 BU Simuliidae 13 197 I LBM BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
ii
jj
Other Total Individuals 66
(69) 1000
Total Taxa 7 (8)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Culicidae (Diptera) pupae not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl J Pangle S Robertson W Bousquet B Millholland J Tovar H Kepple
Collection date 30 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 1 12 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Asellidae 114 965 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Plecoptera (stoneflies) Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 2 23 A MRD BVW SC 4 CG Diptera (true flies)
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
kk
ll
Other Total Individuals 117 1000 Total Taxa 3
Notes Subsample taken 29 October 2009 All taxonomic determinations by MRD were confirmed by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson K McCann A Fisher H Kepple A Barrett
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 2 17 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Sphaeriidae 2 17 I MRD BVW CF 8 BU Isopoda (isopods) Asellidae 99 853 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Calopterygidae 2 17 A MRD BVW PR 7 CL Ephemeroptera (mayflies) Leptophlebiidae 2 17 I MRD BVW CG 2 CR Heptageniidae 4 34 A MRD BVW SC 3 CG Plecoptera (stoneflies) Perlidae 1 09 A MRD BVW PR 1 CR Trichoptera (caddisflies) Hemiptera (true bugs) (Gerridae) (1) - A MRD BVW ( ) Corixidae 1 09 A MRD BVW PR 5 CR Megaloptera (dobsonflies etc) Coleoptera (beetles) Dryopidae 1 09 A MRD BVW SC 5 CG Diptera (true flies) Tipulidae 2 17 I BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
mm
nn
Other Total Individuals 116
(117) 999
Total Taxa 10 (11)
Notes Subsample taken 6 November 2008 Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009 Gerridae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
- Disclaimer ii
- Title Page iii
- Acknowledgements v
- Table of Contents vii
- SECTION 2 RESULTS DATA TABLES 7
- OVERVIEW AND FINDINGS
- Introduction
- RESULTS DATA TABLES
- REFERENCES
- FIELD DATA SHEETS
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- SubstrateType
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- WATER QUALITY ANALYSIS
- THROUGH THE VIRGINIA
- STREAM CONDITION INDEX (VSCI)
- LABORATORY BENCH SHEETS
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
v
Other Total Individuals 86
(91) 999
Total Taxa 10 (11)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009 Veliidae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
w
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-2 STATION NAME Package Plant LAT N 39deg 12958rsquo (upstream) LONG W 078deg 04900 (upstream) LAT N 39deg 12958rsquo (downstream) LONG W 078deg 04822 (downstream) FORM COMPILED BY JW Pangle W Bousquet RIVER BASIN Potomac River INVESTIGATORS JW Pangle S Robertson J Tovar M Dahl B Millholland W Bousquet
Collection date 29 May 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Plecoptera (stoneflies) Perlidae 11 136 I JP BVW PR 1 CR Trichoptera (caddisflies) Hydropsychidae 10 123 I JP BVW CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 14 173 I JP BVW SC 4 CG Diptera (true flies) Chironomidae 32 395 I JP BVW CG 6 BU Simuliidae 14 173 I JP BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
x
Other Total Individuals 81 1000 Total Taxa 5
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-2 STATION NAME Package Plant LAT N 39deg 12958rsquo (upstream) LONG W 078deg 04900 (upstream) LAT N 39deg 12958rsquo (downstream) LONG W 078deg 04822 (downstream) FORM COMPILED BY JW Pangle W Bousquet RIVER BASIN Potomac River INVESTIGATORS JW Pangle Brandon Millholland Jeremy Tovar Heather Kepple
Collection date 10 October 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Heptageniidae 40 392 I WBJP SC 3 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Hydropsychidae 7 69 I JP WB BVW CF 6 CG Philopotamidae 28 275 I JP WB BVW CF 2 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Corydalidae 6 59 I JP WB BVW PR 5 CR Coleoptera (beetles) Psephenidae 7 69 I JP WB BVW SC 4 CG Elmidae 8 78 I JP WB BVW SC 4 CG Diptera (true flies) Tipulidae 6 59 I JP WB BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
y
z
Other Total Individuals 102 1001 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-3 STATION NAME Rt 664 Bridge LAT N 39deg 12934rsquo (upstream) LONG W 078deg 05019rsquo (upstream) LAT N 39deg 12951rsquo (downstream) LONG W 078deg 04958rsquo (downstream) FORM COMPILED BY S Robertson W Bousquet RIVER BASIN Potomac River INVESTIGATORS SRobertson BMillholland WBousquet MDahl JPangle
Collection date 28 May 2008 Sheet completion date 11 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Leptophlebiidae 1 15 I SGR BVW CG 2 CR Baetidae 1 15 I BVW CG 5 CG Plecoptera (stoneflies) Perlidae 3 46 I SGR BVW PR 1 CR Nemouridae 1 15 I BVW SH 3 CR Trichoptera (caddisflies) Hydropsychidae 5 77 I SGR BVW CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 27 415 I SGR BVW SC 4 CG Psephenidae 2 31 A SGR BVW SC 4 CG Diptera (true flies) Simuliidae 5 77 I SGR BVW CF 6 CG Chironomidae 20 308 I SGR BVW CG 6 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
aa
bb
Other Total Individuals 65 999 Total Taxa 9
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-3 STATION NAME Rt 664 Bridge LAT N 39deg 12934rsquo (upstream) LONG W 078deg 05019rsquo (upstream) LAT N 39deg 12951rsquo (downstream) LONG W 078deg 04958rsquo (downstream) FORM COMPILED BY S Robertson RIVER BASIN Potomac River INVESTIGATORS S Robertson M Dahl A Fisher H Kepple
Collection date 10 Oct 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Heptageniidae 2 20 I SGR SC 3 CG Isonychiidae 2 20 I BVW CF 2 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Philopotamidae 36 367 I SGR CF 2 CG Hydropsychidae 47 480 I SGR CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 2 20 A SGR SC 4 CG Psephenidae 8 82 A SGR SC 4 CG Diptera (true flies) Tabanidae 1 10 I SGR PR 7 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
cc
dd
Other Total Individuals 98 999 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-4 STATION NAME Unnamed Tributary LAT (upstream) N 39deg 13035rsquo LONG (upstream) W 078deg 05129rsquo LAT (downstream) N 39deg 13000rsquo LONG (downstream) W 078deg 5086rsquo FORM COMPILED BY A Fisher M Dahl rev W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson A Fisher H Kepple
Collection date 31 May 2008 Sheet completion date rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Flatworms (Turbellaria) Worms (Oligochaeta) Naididae 1 14 - BVW CG 8 BU Leeches (Hirudinea) Snails (Gastropoda) Clams (Bivalvia) Isopods (Isopoda) Asellidae 3 42 A AF BVW CG 8 SP Scuds (Amphipoda) Crayfish Shrimp (Decapoda) Water Mites (Hydracarina) Dragonflies (Odonata Anisoptera) Damselflies (Odonata Zygoptera) Mayflies (Ephemeroptera) Stoneflies (Plecoptera) Perlidae 2 28 I AF BVW PR 1 CR Caddisflies (Trichoptera) True Bugs (Hemiptera) Corixidae 1 14 I AF BVW MP 9 DV Dobsonflies (Megaloptera) Beetles (Coleoptera) Dytiscidae 1 14 I AF BVW PR 6 GN True Flies (Diptera) Chironomidae 61 847 I AF BVW CG 6 BU Simuliidae 3 42 I AF BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
ee
ff
Other Total Individuals 72 1001 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-4 STATION NAME Unnamed Tributary LAT (upstream) N 39deg 13035rsquo LONG (upstream) W 078deg 05129rsquo LAT (downstream) N 39deg 13000rsquo LONG (downstream) W 078deg 5086rsquo FORM COMPILED BY Andrew G M Fisher W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson A Fisher H Kepple
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Flatworms (Turbellaria) Worms (Oligochaeta) Lumbriculidae 2 32 A AF BVW CG 8 BU Leeches (Hirudinea) Snails (Gastropoda) Lymnaeidae 1 16 A BVW CG 9 GN Clams (Bivalvia) Isopods (Isopoda) Scuds (Amphipoda) Crayfish Shrimp (Decapoda) Cambaridae 1 16 A AF BVW GN 5 GN Water Mites (Hydracarina) Dragonflies (Odonata Anisoptera) Damselflies (Odonata Zygoptera) Calopterygidae 4 63 I BVW PR 7 CL Mayflies (Ephemeroptera) Tricorythidae 1 16 I BVW CG 5 SP Stoneflies (Plecoptera) Caddisflies (Trichoptera) Phryganeidae 4 63 I AF BVW SH 4 CR Hydropsychidae 6 95 I AF BVW CF 6 CG I True Bugs (Hemiptera) Corixidae 8 127 I AF BVW MP 9 DV (Veliidae ) (7) - I AF BVW ( ) Dobsonflies (Megaloptera) Sialidae 3 48 I AF BVW PR 7 SP Corydalidae 2 32 I AF BVW PR 5 CR Beetles (Coleoptera) Haliplidae 1 16 A AF BVW MP 8 GN Dysticidae 7 111 I AF BVW PR 6 GN Hydrophilidae 1 16 A AF BVW PR 8 GN Elmidae 3 48 I BVW SC 4 CG True Flies (Diptera) Chironomidae 8 127 I AF BVW CG 6 BU Simuliidae 7 111 I AF BVW CF 6 CG Tipulidae 4 63 I AF BVW SH 4 GU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
gg
hh
Other Total Individuals 63
(70) 1000
Total Taxa 17 (18)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Veliidae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-5 STATION NAME Lick Run LAT N 39deg 12752rsquo (upstream) LONG W 078deg 05685rsquo (upstream) LAT N 39deg 12784rsquo (downstream) LONG W 078deg 05631rsquo (downstream) FORM COMPILED BY Brandon Millholland W Bousquet RIVER BASIN Potomac River INVESTIGATORS J Pangle S Robertson W Bousquet JTovar M Dahl
Collection date 29 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Gomphidae 1 15 I BVW PR 3 BU Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Leptophlebiidae 11 167 A LBM BVW CG 2 CR Plecoptera (stoneflies) Perlidae 10 152 A LBM BVW PR 1 CR Perlodiae 1 15 A LBM BVW PR 2 CR Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 5 76 A LBM BVW SC 4 CG Elmidae 2 30 I LBM BVW SC 4 CG Diptera (true flies) (Culicidae) (3) - P LMB BVW ( ) Chironomidae 23 348 I LBM BVW CG 6 BU Simuliidae 13 197 I LBM BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
ii
jj
Other Total Individuals 66
(69) 1000
Total Taxa 7 (8)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Culicidae (Diptera) pupae not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl J Pangle S Robertson W Bousquet B Millholland J Tovar H Kepple
Collection date 30 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 1 12 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Asellidae 114 965 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Plecoptera (stoneflies) Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 2 23 A MRD BVW SC 4 CG Diptera (true flies)
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
kk
ll
Other Total Individuals 117 1000 Total Taxa 3
Notes Subsample taken 29 October 2009 All taxonomic determinations by MRD were confirmed by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson K McCann A Fisher H Kepple A Barrett
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 2 17 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Sphaeriidae 2 17 I MRD BVW CF 8 BU Isopoda (isopods) Asellidae 99 853 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Calopterygidae 2 17 A MRD BVW PR 7 CL Ephemeroptera (mayflies) Leptophlebiidae 2 17 I MRD BVW CG 2 CR Heptageniidae 4 34 A MRD BVW SC 3 CG Plecoptera (stoneflies) Perlidae 1 09 A MRD BVW PR 1 CR Trichoptera (caddisflies) Hemiptera (true bugs) (Gerridae) (1) - A MRD BVW ( ) Corixidae 1 09 A MRD BVW PR 5 CR Megaloptera (dobsonflies etc) Coleoptera (beetles) Dryopidae 1 09 A MRD BVW SC 5 CG Diptera (true flies) Tipulidae 2 17 I BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
mm
nn
Other Total Individuals 116
(117) 999
Total Taxa 10 (11)
Notes Subsample taken 6 November 2008 Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009 Gerridae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
- Disclaimer ii
- Title Page iii
- Acknowledgements v
- Table of Contents vii
- SECTION 2 RESULTS DATA TABLES 7
- OVERVIEW AND FINDINGS
- Introduction
- RESULTS DATA TABLES
- REFERENCES
- FIELD DATA SHEETS
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- SubstrateType
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- WATER QUALITY ANALYSIS
- THROUGH THE VIRGINIA
- STREAM CONDITION INDEX (VSCI)
- LABORATORY BENCH SHEETS
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
w
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-2 STATION NAME Package Plant LAT N 39deg 12958rsquo (upstream) LONG W 078deg 04900 (upstream) LAT N 39deg 12958rsquo (downstream) LONG W 078deg 04822 (downstream) FORM COMPILED BY JW Pangle W Bousquet RIVER BASIN Potomac River INVESTIGATORS JW Pangle S Robertson J Tovar M Dahl B Millholland W Bousquet
Collection date 29 May 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Plecoptera (stoneflies) Perlidae 11 136 I JP BVW PR 1 CR Trichoptera (caddisflies) Hydropsychidae 10 123 I JP BVW CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 14 173 I JP BVW SC 4 CG Diptera (true flies) Chironomidae 32 395 I JP BVW CG 6 BU Simuliidae 14 173 I JP BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
x
Other Total Individuals 81 1000 Total Taxa 5
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-2 STATION NAME Package Plant LAT N 39deg 12958rsquo (upstream) LONG W 078deg 04900 (upstream) LAT N 39deg 12958rsquo (downstream) LONG W 078deg 04822 (downstream) FORM COMPILED BY JW Pangle W Bousquet RIVER BASIN Potomac River INVESTIGATORS JW Pangle Brandon Millholland Jeremy Tovar Heather Kepple
Collection date 10 October 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Heptageniidae 40 392 I WBJP SC 3 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Hydropsychidae 7 69 I JP WB BVW CF 6 CG Philopotamidae 28 275 I JP WB BVW CF 2 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Corydalidae 6 59 I JP WB BVW PR 5 CR Coleoptera (beetles) Psephenidae 7 69 I JP WB BVW SC 4 CG Elmidae 8 78 I JP WB BVW SC 4 CG Diptera (true flies) Tipulidae 6 59 I JP WB BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
y
z
Other Total Individuals 102 1001 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-3 STATION NAME Rt 664 Bridge LAT N 39deg 12934rsquo (upstream) LONG W 078deg 05019rsquo (upstream) LAT N 39deg 12951rsquo (downstream) LONG W 078deg 04958rsquo (downstream) FORM COMPILED BY S Robertson W Bousquet RIVER BASIN Potomac River INVESTIGATORS SRobertson BMillholland WBousquet MDahl JPangle
Collection date 28 May 2008 Sheet completion date 11 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Leptophlebiidae 1 15 I SGR BVW CG 2 CR Baetidae 1 15 I BVW CG 5 CG Plecoptera (stoneflies) Perlidae 3 46 I SGR BVW PR 1 CR Nemouridae 1 15 I BVW SH 3 CR Trichoptera (caddisflies) Hydropsychidae 5 77 I SGR BVW CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 27 415 I SGR BVW SC 4 CG Psephenidae 2 31 A SGR BVW SC 4 CG Diptera (true flies) Simuliidae 5 77 I SGR BVW CF 6 CG Chironomidae 20 308 I SGR BVW CG 6 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
aa
bb
Other Total Individuals 65 999 Total Taxa 9
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-3 STATION NAME Rt 664 Bridge LAT N 39deg 12934rsquo (upstream) LONG W 078deg 05019rsquo (upstream) LAT N 39deg 12951rsquo (downstream) LONG W 078deg 04958rsquo (downstream) FORM COMPILED BY S Robertson RIVER BASIN Potomac River INVESTIGATORS S Robertson M Dahl A Fisher H Kepple
Collection date 10 Oct 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Heptageniidae 2 20 I SGR SC 3 CG Isonychiidae 2 20 I BVW CF 2 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Philopotamidae 36 367 I SGR CF 2 CG Hydropsychidae 47 480 I SGR CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 2 20 A SGR SC 4 CG Psephenidae 8 82 A SGR SC 4 CG Diptera (true flies) Tabanidae 1 10 I SGR PR 7 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
cc
dd
Other Total Individuals 98 999 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-4 STATION NAME Unnamed Tributary LAT (upstream) N 39deg 13035rsquo LONG (upstream) W 078deg 05129rsquo LAT (downstream) N 39deg 13000rsquo LONG (downstream) W 078deg 5086rsquo FORM COMPILED BY A Fisher M Dahl rev W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson A Fisher H Kepple
Collection date 31 May 2008 Sheet completion date rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Flatworms (Turbellaria) Worms (Oligochaeta) Naididae 1 14 - BVW CG 8 BU Leeches (Hirudinea) Snails (Gastropoda) Clams (Bivalvia) Isopods (Isopoda) Asellidae 3 42 A AF BVW CG 8 SP Scuds (Amphipoda) Crayfish Shrimp (Decapoda) Water Mites (Hydracarina) Dragonflies (Odonata Anisoptera) Damselflies (Odonata Zygoptera) Mayflies (Ephemeroptera) Stoneflies (Plecoptera) Perlidae 2 28 I AF BVW PR 1 CR Caddisflies (Trichoptera) True Bugs (Hemiptera) Corixidae 1 14 I AF BVW MP 9 DV Dobsonflies (Megaloptera) Beetles (Coleoptera) Dytiscidae 1 14 I AF BVW PR 6 GN True Flies (Diptera) Chironomidae 61 847 I AF BVW CG 6 BU Simuliidae 3 42 I AF BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
ee
ff
Other Total Individuals 72 1001 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-4 STATION NAME Unnamed Tributary LAT (upstream) N 39deg 13035rsquo LONG (upstream) W 078deg 05129rsquo LAT (downstream) N 39deg 13000rsquo LONG (downstream) W 078deg 5086rsquo FORM COMPILED BY Andrew G M Fisher W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson A Fisher H Kepple
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Flatworms (Turbellaria) Worms (Oligochaeta) Lumbriculidae 2 32 A AF BVW CG 8 BU Leeches (Hirudinea) Snails (Gastropoda) Lymnaeidae 1 16 A BVW CG 9 GN Clams (Bivalvia) Isopods (Isopoda) Scuds (Amphipoda) Crayfish Shrimp (Decapoda) Cambaridae 1 16 A AF BVW GN 5 GN Water Mites (Hydracarina) Dragonflies (Odonata Anisoptera) Damselflies (Odonata Zygoptera) Calopterygidae 4 63 I BVW PR 7 CL Mayflies (Ephemeroptera) Tricorythidae 1 16 I BVW CG 5 SP Stoneflies (Plecoptera) Caddisflies (Trichoptera) Phryganeidae 4 63 I AF BVW SH 4 CR Hydropsychidae 6 95 I AF BVW CF 6 CG I True Bugs (Hemiptera) Corixidae 8 127 I AF BVW MP 9 DV (Veliidae ) (7) - I AF BVW ( ) Dobsonflies (Megaloptera) Sialidae 3 48 I AF BVW PR 7 SP Corydalidae 2 32 I AF BVW PR 5 CR Beetles (Coleoptera) Haliplidae 1 16 A AF BVW MP 8 GN Dysticidae 7 111 I AF BVW PR 6 GN Hydrophilidae 1 16 A AF BVW PR 8 GN Elmidae 3 48 I BVW SC 4 CG True Flies (Diptera) Chironomidae 8 127 I AF BVW CG 6 BU Simuliidae 7 111 I AF BVW CF 6 CG Tipulidae 4 63 I AF BVW SH 4 GU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
gg
hh
Other Total Individuals 63
(70) 1000
Total Taxa 17 (18)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Veliidae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-5 STATION NAME Lick Run LAT N 39deg 12752rsquo (upstream) LONG W 078deg 05685rsquo (upstream) LAT N 39deg 12784rsquo (downstream) LONG W 078deg 05631rsquo (downstream) FORM COMPILED BY Brandon Millholland W Bousquet RIVER BASIN Potomac River INVESTIGATORS J Pangle S Robertson W Bousquet JTovar M Dahl
Collection date 29 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Gomphidae 1 15 I BVW PR 3 BU Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Leptophlebiidae 11 167 A LBM BVW CG 2 CR Plecoptera (stoneflies) Perlidae 10 152 A LBM BVW PR 1 CR Perlodiae 1 15 A LBM BVW PR 2 CR Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 5 76 A LBM BVW SC 4 CG Elmidae 2 30 I LBM BVW SC 4 CG Diptera (true flies) (Culicidae) (3) - P LMB BVW ( ) Chironomidae 23 348 I LBM BVW CG 6 BU Simuliidae 13 197 I LBM BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
ii
jj
Other Total Individuals 66
(69) 1000
Total Taxa 7 (8)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Culicidae (Diptera) pupae not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl J Pangle S Robertson W Bousquet B Millholland J Tovar H Kepple
Collection date 30 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 1 12 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Asellidae 114 965 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Plecoptera (stoneflies) Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 2 23 A MRD BVW SC 4 CG Diptera (true flies)
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
kk
ll
Other Total Individuals 117 1000 Total Taxa 3
Notes Subsample taken 29 October 2009 All taxonomic determinations by MRD were confirmed by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson K McCann A Fisher H Kepple A Barrett
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 2 17 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Sphaeriidae 2 17 I MRD BVW CF 8 BU Isopoda (isopods) Asellidae 99 853 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Calopterygidae 2 17 A MRD BVW PR 7 CL Ephemeroptera (mayflies) Leptophlebiidae 2 17 I MRD BVW CG 2 CR Heptageniidae 4 34 A MRD BVW SC 3 CG Plecoptera (stoneflies) Perlidae 1 09 A MRD BVW PR 1 CR Trichoptera (caddisflies) Hemiptera (true bugs) (Gerridae) (1) - A MRD BVW ( ) Corixidae 1 09 A MRD BVW PR 5 CR Megaloptera (dobsonflies etc) Coleoptera (beetles) Dryopidae 1 09 A MRD BVW SC 5 CG Diptera (true flies) Tipulidae 2 17 I BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
mm
nn
Other Total Individuals 116
(117) 999
Total Taxa 10 (11)
Notes Subsample taken 6 November 2008 Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009 Gerridae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
- Disclaimer ii
- Title Page iii
- Acknowledgements v
- Table of Contents vii
- SECTION 2 RESULTS DATA TABLES 7
- OVERVIEW AND FINDINGS
- Introduction
- RESULTS DATA TABLES
- REFERENCES
- FIELD DATA SHEETS
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- SubstrateType
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- WATER QUALITY ANALYSIS
- THROUGH THE VIRGINIA
- STREAM CONDITION INDEX (VSCI)
- LABORATORY BENCH SHEETS
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
x
Other Total Individuals 81 1000 Total Taxa 5
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-2 STATION NAME Package Plant LAT N 39deg 12958rsquo (upstream) LONG W 078deg 04900 (upstream) LAT N 39deg 12958rsquo (downstream) LONG W 078deg 04822 (downstream) FORM COMPILED BY JW Pangle W Bousquet RIVER BASIN Potomac River INVESTIGATORS JW Pangle Brandon Millholland Jeremy Tovar Heather Kepple
Collection date 10 October 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Heptageniidae 40 392 I WBJP SC 3 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Hydropsychidae 7 69 I JP WB BVW CF 6 CG Philopotamidae 28 275 I JP WB BVW CF 2 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Corydalidae 6 59 I JP WB BVW PR 5 CR Coleoptera (beetles) Psephenidae 7 69 I JP WB BVW SC 4 CG Elmidae 8 78 I JP WB BVW SC 4 CG Diptera (true flies) Tipulidae 6 59 I JP WB BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
y
z
Other Total Individuals 102 1001 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-3 STATION NAME Rt 664 Bridge LAT N 39deg 12934rsquo (upstream) LONG W 078deg 05019rsquo (upstream) LAT N 39deg 12951rsquo (downstream) LONG W 078deg 04958rsquo (downstream) FORM COMPILED BY S Robertson W Bousquet RIVER BASIN Potomac River INVESTIGATORS SRobertson BMillholland WBousquet MDahl JPangle
Collection date 28 May 2008 Sheet completion date 11 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Leptophlebiidae 1 15 I SGR BVW CG 2 CR Baetidae 1 15 I BVW CG 5 CG Plecoptera (stoneflies) Perlidae 3 46 I SGR BVW PR 1 CR Nemouridae 1 15 I BVW SH 3 CR Trichoptera (caddisflies) Hydropsychidae 5 77 I SGR BVW CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 27 415 I SGR BVW SC 4 CG Psephenidae 2 31 A SGR BVW SC 4 CG Diptera (true flies) Simuliidae 5 77 I SGR BVW CF 6 CG Chironomidae 20 308 I SGR BVW CG 6 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
aa
bb
Other Total Individuals 65 999 Total Taxa 9
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-3 STATION NAME Rt 664 Bridge LAT N 39deg 12934rsquo (upstream) LONG W 078deg 05019rsquo (upstream) LAT N 39deg 12951rsquo (downstream) LONG W 078deg 04958rsquo (downstream) FORM COMPILED BY S Robertson RIVER BASIN Potomac River INVESTIGATORS S Robertson M Dahl A Fisher H Kepple
Collection date 10 Oct 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Heptageniidae 2 20 I SGR SC 3 CG Isonychiidae 2 20 I BVW CF 2 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Philopotamidae 36 367 I SGR CF 2 CG Hydropsychidae 47 480 I SGR CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 2 20 A SGR SC 4 CG Psephenidae 8 82 A SGR SC 4 CG Diptera (true flies) Tabanidae 1 10 I SGR PR 7 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
cc
dd
Other Total Individuals 98 999 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-4 STATION NAME Unnamed Tributary LAT (upstream) N 39deg 13035rsquo LONG (upstream) W 078deg 05129rsquo LAT (downstream) N 39deg 13000rsquo LONG (downstream) W 078deg 5086rsquo FORM COMPILED BY A Fisher M Dahl rev W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson A Fisher H Kepple
Collection date 31 May 2008 Sheet completion date rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Flatworms (Turbellaria) Worms (Oligochaeta) Naididae 1 14 - BVW CG 8 BU Leeches (Hirudinea) Snails (Gastropoda) Clams (Bivalvia) Isopods (Isopoda) Asellidae 3 42 A AF BVW CG 8 SP Scuds (Amphipoda) Crayfish Shrimp (Decapoda) Water Mites (Hydracarina) Dragonflies (Odonata Anisoptera) Damselflies (Odonata Zygoptera) Mayflies (Ephemeroptera) Stoneflies (Plecoptera) Perlidae 2 28 I AF BVW PR 1 CR Caddisflies (Trichoptera) True Bugs (Hemiptera) Corixidae 1 14 I AF BVW MP 9 DV Dobsonflies (Megaloptera) Beetles (Coleoptera) Dytiscidae 1 14 I AF BVW PR 6 GN True Flies (Diptera) Chironomidae 61 847 I AF BVW CG 6 BU Simuliidae 3 42 I AF BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
ee
ff
Other Total Individuals 72 1001 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-4 STATION NAME Unnamed Tributary LAT (upstream) N 39deg 13035rsquo LONG (upstream) W 078deg 05129rsquo LAT (downstream) N 39deg 13000rsquo LONG (downstream) W 078deg 5086rsquo FORM COMPILED BY Andrew G M Fisher W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson A Fisher H Kepple
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Flatworms (Turbellaria) Worms (Oligochaeta) Lumbriculidae 2 32 A AF BVW CG 8 BU Leeches (Hirudinea) Snails (Gastropoda) Lymnaeidae 1 16 A BVW CG 9 GN Clams (Bivalvia) Isopods (Isopoda) Scuds (Amphipoda) Crayfish Shrimp (Decapoda) Cambaridae 1 16 A AF BVW GN 5 GN Water Mites (Hydracarina) Dragonflies (Odonata Anisoptera) Damselflies (Odonata Zygoptera) Calopterygidae 4 63 I BVW PR 7 CL Mayflies (Ephemeroptera) Tricorythidae 1 16 I BVW CG 5 SP Stoneflies (Plecoptera) Caddisflies (Trichoptera) Phryganeidae 4 63 I AF BVW SH 4 CR Hydropsychidae 6 95 I AF BVW CF 6 CG I True Bugs (Hemiptera) Corixidae 8 127 I AF BVW MP 9 DV (Veliidae ) (7) - I AF BVW ( ) Dobsonflies (Megaloptera) Sialidae 3 48 I AF BVW PR 7 SP Corydalidae 2 32 I AF BVW PR 5 CR Beetles (Coleoptera) Haliplidae 1 16 A AF BVW MP 8 GN Dysticidae 7 111 I AF BVW PR 6 GN Hydrophilidae 1 16 A AF BVW PR 8 GN Elmidae 3 48 I BVW SC 4 CG True Flies (Diptera) Chironomidae 8 127 I AF BVW CG 6 BU Simuliidae 7 111 I AF BVW CF 6 CG Tipulidae 4 63 I AF BVW SH 4 GU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
gg
hh
Other Total Individuals 63
(70) 1000
Total Taxa 17 (18)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Veliidae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-5 STATION NAME Lick Run LAT N 39deg 12752rsquo (upstream) LONG W 078deg 05685rsquo (upstream) LAT N 39deg 12784rsquo (downstream) LONG W 078deg 05631rsquo (downstream) FORM COMPILED BY Brandon Millholland W Bousquet RIVER BASIN Potomac River INVESTIGATORS J Pangle S Robertson W Bousquet JTovar M Dahl
Collection date 29 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Gomphidae 1 15 I BVW PR 3 BU Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Leptophlebiidae 11 167 A LBM BVW CG 2 CR Plecoptera (stoneflies) Perlidae 10 152 A LBM BVW PR 1 CR Perlodiae 1 15 A LBM BVW PR 2 CR Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 5 76 A LBM BVW SC 4 CG Elmidae 2 30 I LBM BVW SC 4 CG Diptera (true flies) (Culicidae) (3) - P LMB BVW ( ) Chironomidae 23 348 I LBM BVW CG 6 BU Simuliidae 13 197 I LBM BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
ii
jj
Other Total Individuals 66
(69) 1000
Total Taxa 7 (8)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Culicidae (Diptera) pupae not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl J Pangle S Robertson W Bousquet B Millholland J Tovar H Kepple
Collection date 30 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 1 12 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Asellidae 114 965 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Plecoptera (stoneflies) Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 2 23 A MRD BVW SC 4 CG Diptera (true flies)
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
kk
ll
Other Total Individuals 117 1000 Total Taxa 3
Notes Subsample taken 29 October 2009 All taxonomic determinations by MRD were confirmed by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson K McCann A Fisher H Kepple A Barrett
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 2 17 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Sphaeriidae 2 17 I MRD BVW CF 8 BU Isopoda (isopods) Asellidae 99 853 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Calopterygidae 2 17 A MRD BVW PR 7 CL Ephemeroptera (mayflies) Leptophlebiidae 2 17 I MRD BVW CG 2 CR Heptageniidae 4 34 A MRD BVW SC 3 CG Plecoptera (stoneflies) Perlidae 1 09 A MRD BVW PR 1 CR Trichoptera (caddisflies) Hemiptera (true bugs) (Gerridae) (1) - A MRD BVW ( ) Corixidae 1 09 A MRD BVW PR 5 CR Megaloptera (dobsonflies etc) Coleoptera (beetles) Dryopidae 1 09 A MRD BVW SC 5 CG Diptera (true flies) Tipulidae 2 17 I BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
mm
nn
Other Total Individuals 116
(117) 999
Total Taxa 10 (11)
Notes Subsample taken 6 November 2008 Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009 Gerridae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
- Disclaimer ii
- Title Page iii
- Acknowledgements v
- Table of Contents vii
- SECTION 2 RESULTS DATA TABLES 7
- OVERVIEW AND FINDINGS
- Introduction
- RESULTS DATA TABLES
- REFERENCES
- FIELD DATA SHEETS
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- SubstrateType
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- WATER QUALITY ANALYSIS
- THROUGH THE VIRGINIA
- STREAM CONDITION INDEX (VSCI)
- LABORATORY BENCH SHEETS
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-2 STATION NAME Package Plant LAT N 39deg 12958rsquo (upstream) LONG W 078deg 04900 (upstream) LAT N 39deg 12958rsquo (downstream) LONG W 078deg 04822 (downstream) FORM COMPILED BY JW Pangle W Bousquet RIVER BASIN Potomac River INVESTIGATORS JW Pangle Brandon Millholland Jeremy Tovar Heather Kepple
Collection date 10 October 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Heptageniidae 40 392 I WBJP SC 3 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Hydropsychidae 7 69 I JP WB BVW CF 6 CG Philopotamidae 28 275 I JP WB BVW CF 2 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Corydalidae 6 59 I JP WB BVW PR 5 CR Coleoptera (beetles) Psephenidae 7 69 I JP WB BVW SC 4 CG Elmidae 8 78 I JP WB BVW SC 4 CG Diptera (true flies) Tipulidae 6 59 I JP WB BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
y
z
Other Total Individuals 102 1001 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-3 STATION NAME Rt 664 Bridge LAT N 39deg 12934rsquo (upstream) LONG W 078deg 05019rsquo (upstream) LAT N 39deg 12951rsquo (downstream) LONG W 078deg 04958rsquo (downstream) FORM COMPILED BY S Robertson W Bousquet RIVER BASIN Potomac River INVESTIGATORS SRobertson BMillholland WBousquet MDahl JPangle
Collection date 28 May 2008 Sheet completion date 11 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Leptophlebiidae 1 15 I SGR BVW CG 2 CR Baetidae 1 15 I BVW CG 5 CG Plecoptera (stoneflies) Perlidae 3 46 I SGR BVW PR 1 CR Nemouridae 1 15 I BVW SH 3 CR Trichoptera (caddisflies) Hydropsychidae 5 77 I SGR BVW CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 27 415 I SGR BVW SC 4 CG Psephenidae 2 31 A SGR BVW SC 4 CG Diptera (true flies) Simuliidae 5 77 I SGR BVW CF 6 CG Chironomidae 20 308 I SGR BVW CG 6 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
aa
bb
Other Total Individuals 65 999 Total Taxa 9
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-3 STATION NAME Rt 664 Bridge LAT N 39deg 12934rsquo (upstream) LONG W 078deg 05019rsquo (upstream) LAT N 39deg 12951rsquo (downstream) LONG W 078deg 04958rsquo (downstream) FORM COMPILED BY S Robertson RIVER BASIN Potomac River INVESTIGATORS S Robertson M Dahl A Fisher H Kepple
Collection date 10 Oct 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Heptageniidae 2 20 I SGR SC 3 CG Isonychiidae 2 20 I BVW CF 2 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Philopotamidae 36 367 I SGR CF 2 CG Hydropsychidae 47 480 I SGR CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 2 20 A SGR SC 4 CG Psephenidae 8 82 A SGR SC 4 CG Diptera (true flies) Tabanidae 1 10 I SGR PR 7 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
cc
dd
Other Total Individuals 98 999 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-4 STATION NAME Unnamed Tributary LAT (upstream) N 39deg 13035rsquo LONG (upstream) W 078deg 05129rsquo LAT (downstream) N 39deg 13000rsquo LONG (downstream) W 078deg 5086rsquo FORM COMPILED BY A Fisher M Dahl rev W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson A Fisher H Kepple
Collection date 31 May 2008 Sheet completion date rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Flatworms (Turbellaria) Worms (Oligochaeta) Naididae 1 14 - BVW CG 8 BU Leeches (Hirudinea) Snails (Gastropoda) Clams (Bivalvia) Isopods (Isopoda) Asellidae 3 42 A AF BVW CG 8 SP Scuds (Amphipoda) Crayfish Shrimp (Decapoda) Water Mites (Hydracarina) Dragonflies (Odonata Anisoptera) Damselflies (Odonata Zygoptera) Mayflies (Ephemeroptera) Stoneflies (Plecoptera) Perlidae 2 28 I AF BVW PR 1 CR Caddisflies (Trichoptera) True Bugs (Hemiptera) Corixidae 1 14 I AF BVW MP 9 DV Dobsonflies (Megaloptera) Beetles (Coleoptera) Dytiscidae 1 14 I AF BVW PR 6 GN True Flies (Diptera) Chironomidae 61 847 I AF BVW CG 6 BU Simuliidae 3 42 I AF BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
ee
ff
Other Total Individuals 72 1001 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-4 STATION NAME Unnamed Tributary LAT (upstream) N 39deg 13035rsquo LONG (upstream) W 078deg 05129rsquo LAT (downstream) N 39deg 13000rsquo LONG (downstream) W 078deg 5086rsquo FORM COMPILED BY Andrew G M Fisher W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson A Fisher H Kepple
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Flatworms (Turbellaria) Worms (Oligochaeta) Lumbriculidae 2 32 A AF BVW CG 8 BU Leeches (Hirudinea) Snails (Gastropoda) Lymnaeidae 1 16 A BVW CG 9 GN Clams (Bivalvia) Isopods (Isopoda) Scuds (Amphipoda) Crayfish Shrimp (Decapoda) Cambaridae 1 16 A AF BVW GN 5 GN Water Mites (Hydracarina) Dragonflies (Odonata Anisoptera) Damselflies (Odonata Zygoptera) Calopterygidae 4 63 I BVW PR 7 CL Mayflies (Ephemeroptera) Tricorythidae 1 16 I BVW CG 5 SP Stoneflies (Plecoptera) Caddisflies (Trichoptera) Phryganeidae 4 63 I AF BVW SH 4 CR Hydropsychidae 6 95 I AF BVW CF 6 CG I True Bugs (Hemiptera) Corixidae 8 127 I AF BVW MP 9 DV (Veliidae ) (7) - I AF BVW ( ) Dobsonflies (Megaloptera) Sialidae 3 48 I AF BVW PR 7 SP Corydalidae 2 32 I AF BVW PR 5 CR Beetles (Coleoptera) Haliplidae 1 16 A AF BVW MP 8 GN Dysticidae 7 111 I AF BVW PR 6 GN Hydrophilidae 1 16 A AF BVW PR 8 GN Elmidae 3 48 I BVW SC 4 CG True Flies (Diptera) Chironomidae 8 127 I AF BVW CG 6 BU Simuliidae 7 111 I AF BVW CF 6 CG Tipulidae 4 63 I AF BVW SH 4 GU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
gg
hh
Other Total Individuals 63
(70) 1000
Total Taxa 17 (18)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Veliidae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-5 STATION NAME Lick Run LAT N 39deg 12752rsquo (upstream) LONG W 078deg 05685rsquo (upstream) LAT N 39deg 12784rsquo (downstream) LONG W 078deg 05631rsquo (downstream) FORM COMPILED BY Brandon Millholland W Bousquet RIVER BASIN Potomac River INVESTIGATORS J Pangle S Robertson W Bousquet JTovar M Dahl
Collection date 29 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Gomphidae 1 15 I BVW PR 3 BU Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Leptophlebiidae 11 167 A LBM BVW CG 2 CR Plecoptera (stoneflies) Perlidae 10 152 A LBM BVW PR 1 CR Perlodiae 1 15 A LBM BVW PR 2 CR Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 5 76 A LBM BVW SC 4 CG Elmidae 2 30 I LBM BVW SC 4 CG Diptera (true flies) (Culicidae) (3) - P LMB BVW ( ) Chironomidae 23 348 I LBM BVW CG 6 BU Simuliidae 13 197 I LBM BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
ii
jj
Other Total Individuals 66
(69) 1000
Total Taxa 7 (8)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Culicidae (Diptera) pupae not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl J Pangle S Robertson W Bousquet B Millholland J Tovar H Kepple
Collection date 30 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 1 12 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Asellidae 114 965 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Plecoptera (stoneflies) Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 2 23 A MRD BVW SC 4 CG Diptera (true flies)
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
kk
ll
Other Total Individuals 117 1000 Total Taxa 3
Notes Subsample taken 29 October 2009 All taxonomic determinations by MRD were confirmed by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson K McCann A Fisher H Kepple A Barrett
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 2 17 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Sphaeriidae 2 17 I MRD BVW CF 8 BU Isopoda (isopods) Asellidae 99 853 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Calopterygidae 2 17 A MRD BVW PR 7 CL Ephemeroptera (mayflies) Leptophlebiidae 2 17 I MRD BVW CG 2 CR Heptageniidae 4 34 A MRD BVW SC 3 CG Plecoptera (stoneflies) Perlidae 1 09 A MRD BVW PR 1 CR Trichoptera (caddisflies) Hemiptera (true bugs) (Gerridae) (1) - A MRD BVW ( ) Corixidae 1 09 A MRD BVW PR 5 CR Megaloptera (dobsonflies etc) Coleoptera (beetles) Dryopidae 1 09 A MRD BVW SC 5 CG Diptera (true flies) Tipulidae 2 17 I BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
mm
nn
Other Total Individuals 116
(117) 999
Total Taxa 10 (11)
Notes Subsample taken 6 November 2008 Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009 Gerridae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
- Disclaimer ii
- Title Page iii
- Acknowledgements v
- Table of Contents vii
- SECTION 2 RESULTS DATA TABLES 7
- OVERVIEW AND FINDINGS
- Introduction
- RESULTS DATA TABLES
- REFERENCES
- FIELD DATA SHEETS
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- SubstrateType
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- WATER QUALITY ANALYSIS
- THROUGH THE VIRGINIA
- STREAM CONDITION INDEX (VSCI)
- LABORATORY BENCH SHEETS
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
z
Other Total Individuals 102 1001 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-3 STATION NAME Rt 664 Bridge LAT N 39deg 12934rsquo (upstream) LONG W 078deg 05019rsquo (upstream) LAT N 39deg 12951rsquo (downstream) LONG W 078deg 04958rsquo (downstream) FORM COMPILED BY S Robertson W Bousquet RIVER BASIN Potomac River INVESTIGATORS SRobertson BMillholland WBousquet MDahl JPangle
Collection date 28 May 2008 Sheet completion date 11 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Leptophlebiidae 1 15 I SGR BVW CG 2 CR Baetidae 1 15 I BVW CG 5 CG Plecoptera (stoneflies) Perlidae 3 46 I SGR BVW PR 1 CR Nemouridae 1 15 I BVW SH 3 CR Trichoptera (caddisflies) Hydropsychidae 5 77 I SGR BVW CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 27 415 I SGR BVW SC 4 CG Psephenidae 2 31 A SGR BVW SC 4 CG Diptera (true flies) Simuliidae 5 77 I SGR BVW CF 6 CG Chironomidae 20 308 I SGR BVW CG 6 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
aa
bb
Other Total Individuals 65 999 Total Taxa 9
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-3 STATION NAME Rt 664 Bridge LAT N 39deg 12934rsquo (upstream) LONG W 078deg 05019rsquo (upstream) LAT N 39deg 12951rsquo (downstream) LONG W 078deg 04958rsquo (downstream) FORM COMPILED BY S Robertson RIVER BASIN Potomac River INVESTIGATORS S Robertson M Dahl A Fisher H Kepple
Collection date 10 Oct 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Heptageniidae 2 20 I SGR SC 3 CG Isonychiidae 2 20 I BVW CF 2 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Philopotamidae 36 367 I SGR CF 2 CG Hydropsychidae 47 480 I SGR CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 2 20 A SGR SC 4 CG Psephenidae 8 82 A SGR SC 4 CG Diptera (true flies) Tabanidae 1 10 I SGR PR 7 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
cc
dd
Other Total Individuals 98 999 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-4 STATION NAME Unnamed Tributary LAT (upstream) N 39deg 13035rsquo LONG (upstream) W 078deg 05129rsquo LAT (downstream) N 39deg 13000rsquo LONG (downstream) W 078deg 5086rsquo FORM COMPILED BY A Fisher M Dahl rev W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson A Fisher H Kepple
Collection date 31 May 2008 Sheet completion date rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Flatworms (Turbellaria) Worms (Oligochaeta) Naididae 1 14 - BVW CG 8 BU Leeches (Hirudinea) Snails (Gastropoda) Clams (Bivalvia) Isopods (Isopoda) Asellidae 3 42 A AF BVW CG 8 SP Scuds (Amphipoda) Crayfish Shrimp (Decapoda) Water Mites (Hydracarina) Dragonflies (Odonata Anisoptera) Damselflies (Odonata Zygoptera) Mayflies (Ephemeroptera) Stoneflies (Plecoptera) Perlidae 2 28 I AF BVW PR 1 CR Caddisflies (Trichoptera) True Bugs (Hemiptera) Corixidae 1 14 I AF BVW MP 9 DV Dobsonflies (Megaloptera) Beetles (Coleoptera) Dytiscidae 1 14 I AF BVW PR 6 GN True Flies (Diptera) Chironomidae 61 847 I AF BVW CG 6 BU Simuliidae 3 42 I AF BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
ee
ff
Other Total Individuals 72 1001 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-4 STATION NAME Unnamed Tributary LAT (upstream) N 39deg 13035rsquo LONG (upstream) W 078deg 05129rsquo LAT (downstream) N 39deg 13000rsquo LONG (downstream) W 078deg 5086rsquo FORM COMPILED BY Andrew G M Fisher W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson A Fisher H Kepple
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Flatworms (Turbellaria) Worms (Oligochaeta) Lumbriculidae 2 32 A AF BVW CG 8 BU Leeches (Hirudinea) Snails (Gastropoda) Lymnaeidae 1 16 A BVW CG 9 GN Clams (Bivalvia) Isopods (Isopoda) Scuds (Amphipoda) Crayfish Shrimp (Decapoda) Cambaridae 1 16 A AF BVW GN 5 GN Water Mites (Hydracarina) Dragonflies (Odonata Anisoptera) Damselflies (Odonata Zygoptera) Calopterygidae 4 63 I BVW PR 7 CL Mayflies (Ephemeroptera) Tricorythidae 1 16 I BVW CG 5 SP Stoneflies (Plecoptera) Caddisflies (Trichoptera) Phryganeidae 4 63 I AF BVW SH 4 CR Hydropsychidae 6 95 I AF BVW CF 6 CG I True Bugs (Hemiptera) Corixidae 8 127 I AF BVW MP 9 DV (Veliidae ) (7) - I AF BVW ( ) Dobsonflies (Megaloptera) Sialidae 3 48 I AF BVW PR 7 SP Corydalidae 2 32 I AF BVW PR 5 CR Beetles (Coleoptera) Haliplidae 1 16 A AF BVW MP 8 GN Dysticidae 7 111 I AF BVW PR 6 GN Hydrophilidae 1 16 A AF BVW PR 8 GN Elmidae 3 48 I BVW SC 4 CG True Flies (Diptera) Chironomidae 8 127 I AF BVW CG 6 BU Simuliidae 7 111 I AF BVW CF 6 CG Tipulidae 4 63 I AF BVW SH 4 GU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
gg
hh
Other Total Individuals 63
(70) 1000
Total Taxa 17 (18)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Veliidae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-5 STATION NAME Lick Run LAT N 39deg 12752rsquo (upstream) LONG W 078deg 05685rsquo (upstream) LAT N 39deg 12784rsquo (downstream) LONG W 078deg 05631rsquo (downstream) FORM COMPILED BY Brandon Millholland W Bousquet RIVER BASIN Potomac River INVESTIGATORS J Pangle S Robertson W Bousquet JTovar M Dahl
Collection date 29 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Gomphidae 1 15 I BVW PR 3 BU Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Leptophlebiidae 11 167 A LBM BVW CG 2 CR Plecoptera (stoneflies) Perlidae 10 152 A LBM BVW PR 1 CR Perlodiae 1 15 A LBM BVW PR 2 CR Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 5 76 A LBM BVW SC 4 CG Elmidae 2 30 I LBM BVW SC 4 CG Diptera (true flies) (Culicidae) (3) - P LMB BVW ( ) Chironomidae 23 348 I LBM BVW CG 6 BU Simuliidae 13 197 I LBM BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
ii
jj
Other Total Individuals 66
(69) 1000
Total Taxa 7 (8)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Culicidae (Diptera) pupae not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl J Pangle S Robertson W Bousquet B Millholland J Tovar H Kepple
Collection date 30 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 1 12 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Asellidae 114 965 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Plecoptera (stoneflies) Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 2 23 A MRD BVW SC 4 CG Diptera (true flies)
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
kk
ll
Other Total Individuals 117 1000 Total Taxa 3
Notes Subsample taken 29 October 2009 All taxonomic determinations by MRD were confirmed by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson K McCann A Fisher H Kepple A Barrett
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 2 17 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Sphaeriidae 2 17 I MRD BVW CF 8 BU Isopoda (isopods) Asellidae 99 853 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Calopterygidae 2 17 A MRD BVW PR 7 CL Ephemeroptera (mayflies) Leptophlebiidae 2 17 I MRD BVW CG 2 CR Heptageniidae 4 34 A MRD BVW SC 3 CG Plecoptera (stoneflies) Perlidae 1 09 A MRD BVW PR 1 CR Trichoptera (caddisflies) Hemiptera (true bugs) (Gerridae) (1) - A MRD BVW ( ) Corixidae 1 09 A MRD BVW PR 5 CR Megaloptera (dobsonflies etc) Coleoptera (beetles) Dryopidae 1 09 A MRD BVW SC 5 CG Diptera (true flies) Tipulidae 2 17 I BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
mm
nn
Other Total Individuals 116
(117) 999
Total Taxa 10 (11)
Notes Subsample taken 6 November 2008 Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009 Gerridae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
- Disclaimer ii
- Title Page iii
- Acknowledgements v
- Table of Contents vii
- SECTION 2 RESULTS DATA TABLES 7
- OVERVIEW AND FINDINGS
- Introduction
- RESULTS DATA TABLES
- REFERENCES
- FIELD DATA SHEETS
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- SubstrateType
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- WATER QUALITY ANALYSIS
- THROUGH THE VIRGINIA
- STREAM CONDITION INDEX (VSCI)
- LABORATORY BENCH SHEETS
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-3 STATION NAME Rt 664 Bridge LAT N 39deg 12934rsquo (upstream) LONG W 078deg 05019rsquo (upstream) LAT N 39deg 12951rsquo (downstream) LONG W 078deg 04958rsquo (downstream) FORM COMPILED BY S Robertson W Bousquet RIVER BASIN Potomac River INVESTIGATORS SRobertson BMillholland WBousquet MDahl JPangle
Collection date 28 May 2008 Sheet completion date 11 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Leptophlebiidae 1 15 I SGR BVW CG 2 CR Baetidae 1 15 I BVW CG 5 CG Plecoptera (stoneflies) Perlidae 3 46 I SGR BVW PR 1 CR Nemouridae 1 15 I BVW SH 3 CR Trichoptera (caddisflies) Hydropsychidae 5 77 I SGR BVW CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 27 415 I SGR BVW SC 4 CG Psephenidae 2 31 A SGR BVW SC 4 CG Diptera (true flies) Simuliidae 5 77 I SGR BVW CF 6 CG Chironomidae 20 308 I SGR BVW CG 6 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
aa
bb
Other Total Individuals 65 999 Total Taxa 9
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-3 STATION NAME Rt 664 Bridge LAT N 39deg 12934rsquo (upstream) LONG W 078deg 05019rsquo (upstream) LAT N 39deg 12951rsquo (downstream) LONG W 078deg 04958rsquo (downstream) FORM COMPILED BY S Robertson RIVER BASIN Potomac River INVESTIGATORS S Robertson M Dahl A Fisher H Kepple
Collection date 10 Oct 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Heptageniidae 2 20 I SGR SC 3 CG Isonychiidae 2 20 I BVW CF 2 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Philopotamidae 36 367 I SGR CF 2 CG Hydropsychidae 47 480 I SGR CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 2 20 A SGR SC 4 CG Psephenidae 8 82 A SGR SC 4 CG Diptera (true flies) Tabanidae 1 10 I SGR PR 7 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
cc
dd
Other Total Individuals 98 999 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-4 STATION NAME Unnamed Tributary LAT (upstream) N 39deg 13035rsquo LONG (upstream) W 078deg 05129rsquo LAT (downstream) N 39deg 13000rsquo LONG (downstream) W 078deg 5086rsquo FORM COMPILED BY A Fisher M Dahl rev W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson A Fisher H Kepple
Collection date 31 May 2008 Sheet completion date rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Flatworms (Turbellaria) Worms (Oligochaeta) Naididae 1 14 - BVW CG 8 BU Leeches (Hirudinea) Snails (Gastropoda) Clams (Bivalvia) Isopods (Isopoda) Asellidae 3 42 A AF BVW CG 8 SP Scuds (Amphipoda) Crayfish Shrimp (Decapoda) Water Mites (Hydracarina) Dragonflies (Odonata Anisoptera) Damselflies (Odonata Zygoptera) Mayflies (Ephemeroptera) Stoneflies (Plecoptera) Perlidae 2 28 I AF BVW PR 1 CR Caddisflies (Trichoptera) True Bugs (Hemiptera) Corixidae 1 14 I AF BVW MP 9 DV Dobsonflies (Megaloptera) Beetles (Coleoptera) Dytiscidae 1 14 I AF BVW PR 6 GN True Flies (Diptera) Chironomidae 61 847 I AF BVW CG 6 BU Simuliidae 3 42 I AF BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
ee
ff
Other Total Individuals 72 1001 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-4 STATION NAME Unnamed Tributary LAT (upstream) N 39deg 13035rsquo LONG (upstream) W 078deg 05129rsquo LAT (downstream) N 39deg 13000rsquo LONG (downstream) W 078deg 5086rsquo FORM COMPILED BY Andrew G M Fisher W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson A Fisher H Kepple
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Flatworms (Turbellaria) Worms (Oligochaeta) Lumbriculidae 2 32 A AF BVW CG 8 BU Leeches (Hirudinea) Snails (Gastropoda) Lymnaeidae 1 16 A BVW CG 9 GN Clams (Bivalvia) Isopods (Isopoda) Scuds (Amphipoda) Crayfish Shrimp (Decapoda) Cambaridae 1 16 A AF BVW GN 5 GN Water Mites (Hydracarina) Dragonflies (Odonata Anisoptera) Damselflies (Odonata Zygoptera) Calopterygidae 4 63 I BVW PR 7 CL Mayflies (Ephemeroptera) Tricorythidae 1 16 I BVW CG 5 SP Stoneflies (Plecoptera) Caddisflies (Trichoptera) Phryganeidae 4 63 I AF BVW SH 4 CR Hydropsychidae 6 95 I AF BVW CF 6 CG I True Bugs (Hemiptera) Corixidae 8 127 I AF BVW MP 9 DV (Veliidae ) (7) - I AF BVW ( ) Dobsonflies (Megaloptera) Sialidae 3 48 I AF BVW PR 7 SP Corydalidae 2 32 I AF BVW PR 5 CR Beetles (Coleoptera) Haliplidae 1 16 A AF BVW MP 8 GN Dysticidae 7 111 I AF BVW PR 6 GN Hydrophilidae 1 16 A AF BVW PR 8 GN Elmidae 3 48 I BVW SC 4 CG True Flies (Diptera) Chironomidae 8 127 I AF BVW CG 6 BU Simuliidae 7 111 I AF BVW CF 6 CG Tipulidae 4 63 I AF BVW SH 4 GU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
gg
hh
Other Total Individuals 63
(70) 1000
Total Taxa 17 (18)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Veliidae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-5 STATION NAME Lick Run LAT N 39deg 12752rsquo (upstream) LONG W 078deg 05685rsquo (upstream) LAT N 39deg 12784rsquo (downstream) LONG W 078deg 05631rsquo (downstream) FORM COMPILED BY Brandon Millholland W Bousquet RIVER BASIN Potomac River INVESTIGATORS J Pangle S Robertson W Bousquet JTovar M Dahl
Collection date 29 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Gomphidae 1 15 I BVW PR 3 BU Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Leptophlebiidae 11 167 A LBM BVW CG 2 CR Plecoptera (stoneflies) Perlidae 10 152 A LBM BVW PR 1 CR Perlodiae 1 15 A LBM BVW PR 2 CR Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 5 76 A LBM BVW SC 4 CG Elmidae 2 30 I LBM BVW SC 4 CG Diptera (true flies) (Culicidae) (3) - P LMB BVW ( ) Chironomidae 23 348 I LBM BVW CG 6 BU Simuliidae 13 197 I LBM BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
ii
jj
Other Total Individuals 66
(69) 1000
Total Taxa 7 (8)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Culicidae (Diptera) pupae not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl J Pangle S Robertson W Bousquet B Millholland J Tovar H Kepple
Collection date 30 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 1 12 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Asellidae 114 965 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Plecoptera (stoneflies) Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 2 23 A MRD BVW SC 4 CG Diptera (true flies)
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
kk
ll
Other Total Individuals 117 1000 Total Taxa 3
Notes Subsample taken 29 October 2009 All taxonomic determinations by MRD were confirmed by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson K McCann A Fisher H Kepple A Barrett
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 2 17 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Sphaeriidae 2 17 I MRD BVW CF 8 BU Isopoda (isopods) Asellidae 99 853 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Calopterygidae 2 17 A MRD BVW PR 7 CL Ephemeroptera (mayflies) Leptophlebiidae 2 17 I MRD BVW CG 2 CR Heptageniidae 4 34 A MRD BVW SC 3 CG Plecoptera (stoneflies) Perlidae 1 09 A MRD BVW PR 1 CR Trichoptera (caddisflies) Hemiptera (true bugs) (Gerridae) (1) - A MRD BVW ( ) Corixidae 1 09 A MRD BVW PR 5 CR Megaloptera (dobsonflies etc) Coleoptera (beetles) Dryopidae 1 09 A MRD BVW SC 5 CG Diptera (true flies) Tipulidae 2 17 I BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
mm
nn
Other Total Individuals 116
(117) 999
Total Taxa 10 (11)
Notes Subsample taken 6 November 2008 Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009 Gerridae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
- Disclaimer ii
- Title Page iii
- Acknowledgements v
- Table of Contents vii
- SECTION 2 RESULTS DATA TABLES 7
- OVERVIEW AND FINDINGS
- Introduction
- RESULTS DATA TABLES
- REFERENCES
- FIELD DATA SHEETS
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- SubstrateType
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- WATER QUALITY ANALYSIS
- THROUGH THE VIRGINIA
- STREAM CONDITION INDEX (VSCI)
- LABORATORY BENCH SHEETS
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
bb
Other Total Individuals 65 999 Total Taxa 9
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-3 STATION NAME Rt 664 Bridge LAT N 39deg 12934rsquo (upstream) LONG W 078deg 05019rsquo (upstream) LAT N 39deg 12951rsquo (downstream) LONG W 078deg 04958rsquo (downstream) FORM COMPILED BY S Robertson RIVER BASIN Potomac River INVESTIGATORS S Robertson M Dahl A Fisher H Kepple
Collection date 10 Oct 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Heptageniidae 2 20 I SGR SC 3 CG Isonychiidae 2 20 I BVW CF 2 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Philopotamidae 36 367 I SGR CF 2 CG Hydropsychidae 47 480 I SGR CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 2 20 A SGR SC 4 CG Psephenidae 8 82 A SGR SC 4 CG Diptera (true flies) Tabanidae 1 10 I SGR PR 7 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
cc
dd
Other Total Individuals 98 999 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-4 STATION NAME Unnamed Tributary LAT (upstream) N 39deg 13035rsquo LONG (upstream) W 078deg 05129rsquo LAT (downstream) N 39deg 13000rsquo LONG (downstream) W 078deg 5086rsquo FORM COMPILED BY A Fisher M Dahl rev W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson A Fisher H Kepple
Collection date 31 May 2008 Sheet completion date rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Flatworms (Turbellaria) Worms (Oligochaeta) Naididae 1 14 - BVW CG 8 BU Leeches (Hirudinea) Snails (Gastropoda) Clams (Bivalvia) Isopods (Isopoda) Asellidae 3 42 A AF BVW CG 8 SP Scuds (Amphipoda) Crayfish Shrimp (Decapoda) Water Mites (Hydracarina) Dragonflies (Odonata Anisoptera) Damselflies (Odonata Zygoptera) Mayflies (Ephemeroptera) Stoneflies (Plecoptera) Perlidae 2 28 I AF BVW PR 1 CR Caddisflies (Trichoptera) True Bugs (Hemiptera) Corixidae 1 14 I AF BVW MP 9 DV Dobsonflies (Megaloptera) Beetles (Coleoptera) Dytiscidae 1 14 I AF BVW PR 6 GN True Flies (Diptera) Chironomidae 61 847 I AF BVW CG 6 BU Simuliidae 3 42 I AF BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
ee
ff
Other Total Individuals 72 1001 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-4 STATION NAME Unnamed Tributary LAT (upstream) N 39deg 13035rsquo LONG (upstream) W 078deg 05129rsquo LAT (downstream) N 39deg 13000rsquo LONG (downstream) W 078deg 5086rsquo FORM COMPILED BY Andrew G M Fisher W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson A Fisher H Kepple
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Flatworms (Turbellaria) Worms (Oligochaeta) Lumbriculidae 2 32 A AF BVW CG 8 BU Leeches (Hirudinea) Snails (Gastropoda) Lymnaeidae 1 16 A BVW CG 9 GN Clams (Bivalvia) Isopods (Isopoda) Scuds (Amphipoda) Crayfish Shrimp (Decapoda) Cambaridae 1 16 A AF BVW GN 5 GN Water Mites (Hydracarina) Dragonflies (Odonata Anisoptera) Damselflies (Odonata Zygoptera) Calopterygidae 4 63 I BVW PR 7 CL Mayflies (Ephemeroptera) Tricorythidae 1 16 I BVW CG 5 SP Stoneflies (Plecoptera) Caddisflies (Trichoptera) Phryganeidae 4 63 I AF BVW SH 4 CR Hydropsychidae 6 95 I AF BVW CF 6 CG I True Bugs (Hemiptera) Corixidae 8 127 I AF BVW MP 9 DV (Veliidae ) (7) - I AF BVW ( ) Dobsonflies (Megaloptera) Sialidae 3 48 I AF BVW PR 7 SP Corydalidae 2 32 I AF BVW PR 5 CR Beetles (Coleoptera) Haliplidae 1 16 A AF BVW MP 8 GN Dysticidae 7 111 I AF BVW PR 6 GN Hydrophilidae 1 16 A AF BVW PR 8 GN Elmidae 3 48 I BVW SC 4 CG True Flies (Diptera) Chironomidae 8 127 I AF BVW CG 6 BU Simuliidae 7 111 I AF BVW CF 6 CG Tipulidae 4 63 I AF BVW SH 4 GU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
gg
hh
Other Total Individuals 63
(70) 1000
Total Taxa 17 (18)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Veliidae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-5 STATION NAME Lick Run LAT N 39deg 12752rsquo (upstream) LONG W 078deg 05685rsquo (upstream) LAT N 39deg 12784rsquo (downstream) LONG W 078deg 05631rsquo (downstream) FORM COMPILED BY Brandon Millholland W Bousquet RIVER BASIN Potomac River INVESTIGATORS J Pangle S Robertson W Bousquet JTovar M Dahl
Collection date 29 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Gomphidae 1 15 I BVW PR 3 BU Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Leptophlebiidae 11 167 A LBM BVW CG 2 CR Plecoptera (stoneflies) Perlidae 10 152 A LBM BVW PR 1 CR Perlodiae 1 15 A LBM BVW PR 2 CR Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 5 76 A LBM BVW SC 4 CG Elmidae 2 30 I LBM BVW SC 4 CG Diptera (true flies) (Culicidae) (3) - P LMB BVW ( ) Chironomidae 23 348 I LBM BVW CG 6 BU Simuliidae 13 197 I LBM BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
ii
jj
Other Total Individuals 66
(69) 1000
Total Taxa 7 (8)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Culicidae (Diptera) pupae not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl J Pangle S Robertson W Bousquet B Millholland J Tovar H Kepple
Collection date 30 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 1 12 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Asellidae 114 965 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Plecoptera (stoneflies) Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 2 23 A MRD BVW SC 4 CG Diptera (true flies)
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
kk
ll
Other Total Individuals 117 1000 Total Taxa 3
Notes Subsample taken 29 October 2009 All taxonomic determinations by MRD were confirmed by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson K McCann A Fisher H Kepple A Barrett
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 2 17 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Sphaeriidae 2 17 I MRD BVW CF 8 BU Isopoda (isopods) Asellidae 99 853 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Calopterygidae 2 17 A MRD BVW PR 7 CL Ephemeroptera (mayflies) Leptophlebiidae 2 17 I MRD BVW CG 2 CR Heptageniidae 4 34 A MRD BVW SC 3 CG Plecoptera (stoneflies) Perlidae 1 09 A MRD BVW PR 1 CR Trichoptera (caddisflies) Hemiptera (true bugs) (Gerridae) (1) - A MRD BVW ( ) Corixidae 1 09 A MRD BVW PR 5 CR Megaloptera (dobsonflies etc) Coleoptera (beetles) Dryopidae 1 09 A MRD BVW SC 5 CG Diptera (true flies) Tipulidae 2 17 I BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
mm
nn
Other Total Individuals 116
(117) 999
Total Taxa 10 (11)
Notes Subsample taken 6 November 2008 Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009 Gerridae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
- Disclaimer ii
- Title Page iii
- Acknowledgements v
- Table of Contents vii
- SECTION 2 RESULTS DATA TABLES 7
- OVERVIEW AND FINDINGS
- Introduction
- RESULTS DATA TABLES
- REFERENCES
- FIELD DATA SHEETS
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- SubstrateType
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- WATER QUALITY ANALYSIS
- THROUGH THE VIRGINIA
- STREAM CONDITION INDEX (VSCI)
- LABORATORY BENCH SHEETS
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-3 STATION NAME Rt 664 Bridge LAT N 39deg 12934rsquo (upstream) LONG W 078deg 05019rsquo (upstream) LAT N 39deg 12951rsquo (downstream) LONG W 078deg 04958rsquo (downstream) FORM COMPILED BY S Robertson RIVER BASIN Potomac River INVESTIGATORS S Robertson M Dahl A Fisher H Kepple
Collection date 10 Oct 2008 Sheet completion date 10 December 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Heptageniidae 2 20 I SGR SC 3 CG Isonychiidae 2 20 I BVW CF 2 CG Plecoptera (stoneflies) Trichoptera (caddisflies) Philopotamidae 36 367 I SGR CF 2 CG Hydropsychidae 47 480 I SGR CF 6 CG Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 2 20 A SGR SC 4 CG Psephenidae 8 82 A SGR SC 4 CG Diptera (true flies) Tabanidae 1 10 I SGR PR 7 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
cc
dd
Other Total Individuals 98 999 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-4 STATION NAME Unnamed Tributary LAT (upstream) N 39deg 13035rsquo LONG (upstream) W 078deg 05129rsquo LAT (downstream) N 39deg 13000rsquo LONG (downstream) W 078deg 5086rsquo FORM COMPILED BY A Fisher M Dahl rev W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson A Fisher H Kepple
Collection date 31 May 2008 Sheet completion date rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Flatworms (Turbellaria) Worms (Oligochaeta) Naididae 1 14 - BVW CG 8 BU Leeches (Hirudinea) Snails (Gastropoda) Clams (Bivalvia) Isopods (Isopoda) Asellidae 3 42 A AF BVW CG 8 SP Scuds (Amphipoda) Crayfish Shrimp (Decapoda) Water Mites (Hydracarina) Dragonflies (Odonata Anisoptera) Damselflies (Odonata Zygoptera) Mayflies (Ephemeroptera) Stoneflies (Plecoptera) Perlidae 2 28 I AF BVW PR 1 CR Caddisflies (Trichoptera) True Bugs (Hemiptera) Corixidae 1 14 I AF BVW MP 9 DV Dobsonflies (Megaloptera) Beetles (Coleoptera) Dytiscidae 1 14 I AF BVW PR 6 GN True Flies (Diptera) Chironomidae 61 847 I AF BVW CG 6 BU Simuliidae 3 42 I AF BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
ee
ff
Other Total Individuals 72 1001 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-4 STATION NAME Unnamed Tributary LAT (upstream) N 39deg 13035rsquo LONG (upstream) W 078deg 05129rsquo LAT (downstream) N 39deg 13000rsquo LONG (downstream) W 078deg 5086rsquo FORM COMPILED BY Andrew G M Fisher W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson A Fisher H Kepple
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Flatworms (Turbellaria) Worms (Oligochaeta) Lumbriculidae 2 32 A AF BVW CG 8 BU Leeches (Hirudinea) Snails (Gastropoda) Lymnaeidae 1 16 A BVW CG 9 GN Clams (Bivalvia) Isopods (Isopoda) Scuds (Amphipoda) Crayfish Shrimp (Decapoda) Cambaridae 1 16 A AF BVW GN 5 GN Water Mites (Hydracarina) Dragonflies (Odonata Anisoptera) Damselflies (Odonata Zygoptera) Calopterygidae 4 63 I BVW PR 7 CL Mayflies (Ephemeroptera) Tricorythidae 1 16 I BVW CG 5 SP Stoneflies (Plecoptera) Caddisflies (Trichoptera) Phryganeidae 4 63 I AF BVW SH 4 CR Hydropsychidae 6 95 I AF BVW CF 6 CG I True Bugs (Hemiptera) Corixidae 8 127 I AF BVW MP 9 DV (Veliidae ) (7) - I AF BVW ( ) Dobsonflies (Megaloptera) Sialidae 3 48 I AF BVW PR 7 SP Corydalidae 2 32 I AF BVW PR 5 CR Beetles (Coleoptera) Haliplidae 1 16 A AF BVW MP 8 GN Dysticidae 7 111 I AF BVW PR 6 GN Hydrophilidae 1 16 A AF BVW PR 8 GN Elmidae 3 48 I BVW SC 4 CG True Flies (Diptera) Chironomidae 8 127 I AF BVW CG 6 BU Simuliidae 7 111 I AF BVW CF 6 CG Tipulidae 4 63 I AF BVW SH 4 GU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
gg
hh
Other Total Individuals 63
(70) 1000
Total Taxa 17 (18)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Veliidae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-5 STATION NAME Lick Run LAT N 39deg 12752rsquo (upstream) LONG W 078deg 05685rsquo (upstream) LAT N 39deg 12784rsquo (downstream) LONG W 078deg 05631rsquo (downstream) FORM COMPILED BY Brandon Millholland W Bousquet RIVER BASIN Potomac River INVESTIGATORS J Pangle S Robertson W Bousquet JTovar M Dahl
Collection date 29 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Gomphidae 1 15 I BVW PR 3 BU Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Leptophlebiidae 11 167 A LBM BVW CG 2 CR Plecoptera (stoneflies) Perlidae 10 152 A LBM BVW PR 1 CR Perlodiae 1 15 A LBM BVW PR 2 CR Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 5 76 A LBM BVW SC 4 CG Elmidae 2 30 I LBM BVW SC 4 CG Diptera (true flies) (Culicidae) (3) - P LMB BVW ( ) Chironomidae 23 348 I LBM BVW CG 6 BU Simuliidae 13 197 I LBM BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
ii
jj
Other Total Individuals 66
(69) 1000
Total Taxa 7 (8)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Culicidae (Diptera) pupae not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl J Pangle S Robertson W Bousquet B Millholland J Tovar H Kepple
Collection date 30 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 1 12 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Asellidae 114 965 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Plecoptera (stoneflies) Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 2 23 A MRD BVW SC 4 CG Diptera (true flies)
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
kk
ll
Other Total Individuals 117 1000 Total Taxa 3
Notes Subsample taken 29 October 2009 All taxonomic determinations by MRD were confirmed by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson K McCann A Fisher H Kepple A Barrett
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 2 17 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Sphaeriidae 2 17 I MRD BVW CF 8 BU Isopoda (isopods) Asellidae 99 853 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Calopterygidae 2 17 A MRD BVW PR 7 CL Ephemeroptera (mayflies) Leptophlebiidae 2 17 I MRD BVW CG 2 CR Heptageniidae 4 34 A MRD BVW SC 3 CG Plecoptera (stoneflies) Perlidae 1 09 A MRD BVW PR 1 CR Trichoptera (caddisflies) Hemiptera (true bugs) (Gerridae) (1) - A MRD BVW ( ) Corixidae 1 09 A MRD BVW PR 5 CR Megaloptera (dobsonflies etc) Coleoptera (beetles) Dryopidae 1 09 A MRD BVW SC 5 CG Diptera (true flies) Tipulidae 2 17 I BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
mm
nn
Other Total Individuals 116
(117) 999
Total Taxa 10 (11)
Notes Subsample taken 6 November 2008 Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009 Gerridae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
- Disclaimer ii
- Title Page iii
- Acknowledgements v
- Table of Contents vii
- SECTION 2 RESULTS DATA TABLES 7
- OVERVIEW AND FINDINGS
- Introduction
- RESULTS DATA TABLES
- REFERENCES
- FIELD DATA SHEETS
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- SubstrateType
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- WATER QUALITY ANALYSIS
- THROUGH THE VIRGINIA
- STREAM CONDITION INDEX (VSCI)
- LABORATORY BENCH SHEETS
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
dd
Other Total Individuals 98 999 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-4 STATION NAME Unnamed Tributary LAT (upstream) N 39deg 13035rsquo LONG (upstream) W 078deg 05129rsquo LAT (downstream) N 39deg 13000rsquo LONG (downstream) W 078deg 5086rsquo FORM COMPILED BY A Fisher M Dahl rev W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson A Fisher H Kepple
Collection date 31 May 2008 Sheet completion date rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Flatworms (Turbellaria) Worms (Oligochaeta) Naididae 1 14 - BVW CG 8 BU Leeches (Hirudinea) Snails (Gastropoda) Clams (Bivalvia) Isopods (Isopoda) Asellidae 3 42 A AF BVW CG 8 SP Scuds (Amphipoda) Crayfish Shrimp (Decapoda) Water Mites (Hydracarina) Dragonflies (Odonata Anisoptera) Damselflies (Odonata Zygoptera) Mayflies (Ephemeroptera) Stoneflies (Plecoptera) Perlidae 2 28 I AF BVW PR 1 CR Caddisflies (Trichoptera) True Bugs (Hemiptera) Corixidae 1 14 I AF BVW MP 9 DV Dobsonflies (Megaloptera) Beetles (Coleoptera) Dytiscidae 1 14 I AF BVW PR 6 GN True Flies (Diptera) Chironomidae 61 847 I AF BVW CG 6 BU Simuliidae 3 42 I AF BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
ee
ff
Other Total Individuals 72 1001 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-4 STATION NAME Unnamed Tributary LAT (upstream) N 39deg 13035rsquo LONG (upstream) W 078deg 05129rsquo LAT (downstream) N 39deg 13000rsquo LONG (downstream) W 078deg 5086rsquo FORM COMPILED BY Andrew G M Fisher W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson A Fisher H Kepple
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Flatworms (Turbellaria) Worms (Oligochaeta) Lumbriculidae 2 32 A AF BVW CG 8 BU Leeches (Hirudinea) Snails (Gastropoda) Lymnaeidae 1 16 A BVW CG 9 GN Clams (Bivalvia) Isopods (Isopoda) Scuds (Amphipoda) Crayfish Shrimp (Decapoda) Cambaridae 1 16 A AF BVW GN 5 GN Water Mites (Hydracarina) Dragonflies (Odonata Anisoptera) Damselflies (Odonata Zygoptera) Calopterygidae 4 63 I BVW PR 7 CL Mayflies (Ephemeroptera) Tricorythidae 1 16 I BVW CG 5 SP Stoneflies (Plecoptera) Caddisflies (Trichoptera) Phryganeidae 4 63 I AF BVW SH 4 CR Hydropsychidae 6 95 I AF BVW CF 6 CG I True Bugs (Hemiptera) Corixidae 8 127 I AF BVW MP 9 DV (Veliidae ) (7) - I AF BVW ( ) Dobsonflies (Megaloptera) Sialidae 3 48 I AF BVW PR 7 SP Corydalidae 2 32 I AF BVW PR 5 CR Beetles (Coleoptera) Haliplidae 1 16 A AF BVW MP 8 GN Dysticidae 7 111 I AF BVW PR 6 GN Hydrophilidae 1 16 A AF BVW PR 8 GN Elmidae 3 48 I BVW SC 4 CG True Flies (Diptera) Chironomidae 8 127 I AF BVW CG 6 BU Simuliidae 7 111 I AF BVW CF 6 CG Tipulidae 4 63 I AF BVW SH 4 GU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
gg
hh
Other Total Individuals 63
(70) 1000
Total Taxa 17 (18)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Veliidae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-5 STATION NAME Lick Run LAT N 39deg 12752rsquo (upstream) LONG W 078deg 05685rsquo (upstream) LAT N 39deg 12784rsquo (downstream) LONG W 078deg 05631rsquo (downstream) FORM COMPILED BY Brandon Millholland W Bousquet RIVER BASIN Potomac River INVESTIGATORS J Pangle S Robertson W Bousquet JTovar M Dahl
Collection date 29 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Gomphidae 1 15 I BVW PR 3 BU Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Leptophlebiidae 11 167 A LBM BVW CG 2 CR Plecoptera (stoneflies) Perlidae 10 152 A LBM BVW PR 1 CR Perlodiae 1 15 A LBM BVW PR 2 CR Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 5 76 A LBM BVW SC 4 CG Elmidae 2 30 I LBM BVW SC 4 CG Diptera (true flies) (Culicidae) (3) - P LMB BVW ( ) Chironomidae 23 348 I LBM BVW CG 6 BU Simuliidae 13 197 I LBM BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
ii
jj
Other Total Individuals 66
(69) 1000
Total Taxa 7 (8)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Culicidae (Diptera) pupae not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl J Pangle S Robertson W Bousquet B Millholland J Tovar H Kepple
Collection date 30 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 1 12 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Asellidae 114 965 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Plecoptera (stoneflies) Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 2 23 A MRD BVW SC 4 CG Diptera (true flies)
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
kk
ll
Other Total Individuals 117 1000 Total Taxa 3
Notes Subsample taken 29 October 2009 All taxonomic determinations by MRD were confirmed by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson K McCann A Fisher H Kepple A Barrett
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 2 17 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Sphaeriidae 2 17 I MRD BVW CF 8 BU Isopoda (isopods) Asellidae 99 853 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Calopterygidae 2 17 A MRD BVW PR 7 CL Ephemeroptera (mayflies) Leptophlebiidae 2 17 I MRD BVW CG 2 CR Heptageniidae 4 34 A MRD BVW SC 3 CG Plecoptera (stoneflies) Perlidae 1 09 A MRD BVW PR 1 CR Trichoptera (caddisflies) Hemiptera (true bugs) (Gerridae) (1) - A MRD BVW ( ) Corixidae 1 09 A MRD BVW PR 5 CR Megaloptera (dobsonflies etc) Coleoptera (beetles) Dryopidae 1 09 A MRD BVW SC 5 CG Diptera (true flies) Tipulidae 2 17 I BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
mm
nn
Other Total Individuals 116
(117) 999
Total Taxa 10 (11)
Notes Subsample taken 6 November 2008 Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009 Gerridae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
- Disclaimer ii
- Title Page iii
- Acknowledgements v
- Table of Contents vii
- SECTION 2 RESULTS DATA TABLES 7
- OVERVIEW AND FINDINGS
- Introduction
- RESULTS DATA TABLES
- REFERENCES
- FIELD DATA SHEETS
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- SubstrateType
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- WATER QUALITY ANALYSIS
- THROUGH THE VIRGINIA
- STREAM CONDITION INDEX (VSCI)
- LABORATORY BENCH SHEETS
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-4 STATION NAME Unnamed Tributary LAT (upstream) N 39deg 13035rsquo LONG (upstream) W 078deg 05129rsquo LAT (downstream) N 39deg 13000rsquo LONG (downstream) W 078deg 5086rsquo FORM COMPILED BY A Fisher M Dahl rev W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson A Fisher H Kepple
Collection date 31 May 2008 Sheet completion date rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Flatworms (Turbellaria) Worms (Oligochaeta) Naididae 1 14 - BVW CG 8 BU Leeches (Hirudinea) Snails (Gastropoda) Clams (Bivalvia) Isopods (Isopoda) Asellidae 3 42 A AF BVW CG 8 SP Scuds (Amphipoda) Crayfish Shrimp (Decapoda) Water Mites (Hydracarina) Dragonflies (Odonata Anisoptera) Damselflies (Odonata Zygoptera) Mayflies (Ephemeroptera) Stoneflies (Plecoptera) Perlidae 2 28 I AF BVW PR 1 CR Caddisflies (Trichoptera) True Bugs (Hemiptera) Corixidae 1 14 I AF BVW MP 9 DV Dobsonflies (Megaloptera) Beetles (Coleoptera) Dytiscidae 1 14 I AF BVW PR 6 GN True Flies (Diptera) Chironomidae 61 847 I AF BVW CG 6 BU Simuliidae 3 42 I AF BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
ee
ff
Other Total Individuals 72 1001 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-4 STATION NAME Unnamed Tributary LAT (upstream) N 39deg 13035rsquo LONG (upstream) W 078deg 05129rsquo LAT (downstream) N 39deg 13000rsquo LONG (downstream) W 078deg 5086rsquo FORM COMPILED BY Andrew G M Fisher W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson A Fisher H Kepple
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Flatworms (Turbellaria) Worms (Oligochaeta) Lumbriculidae 2 32 A AF BVW CG 8 BU Leeches (Hirudinea) Snails (Gastropoda) Lymnaeidae 1 16 A BVW CG 9 GN Clams (Bivalvia) Isopods (Isopoda) Scuds (Amphipoda) Crayfish Shrimp (Decapoda) Cambaridae 1 16 A AF BVW GN 5 GN Water Mites (Hydracarina) Dragonflies (Odonata Anisoptera) Damselflies (Odonata Zygoptera) Calopterygidae 4 63 I BVW PR 7 CL Mayflies (Ephemeroptera) Tricorythidae 1 16 I BVW CG 5 SP Stoneflies (Plecoptera) Caddisflies (Trichoptera) Phryganeidae 4 63 I AF BVW SH 4 CR Hydropsychidae 6 95 I AF BVW CF 6 CG I True Bugs (Hemiptera) Corixidae 8 127 I AF BVW MP 9 DV (Veliidae ) (7) - I AF BVW ( ) Dobsonflies (Megaloptera) Sialidae 3 48 I AF BVW PR 7 SP Corydalidae 2 32 I AF BVW PR 5 CR Beetles (Coleoptera) Haliplidae 1 16 A AF BVW MP 8 GN Dysticidae 7 111 I AF BVW PR 6 GN Hydrophilidae 1 16 A AF BVW PR 8 GN Elmidae 3 48 I BVW SC 4 CG True Flies (Diptera) Chironomidae 8 127 I AF BVW CG 6 BU Simuliidae 7 111 I AF BVW CF 6 CG Tipulidae 4 63 I AF BVW SH 4 GU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
gg
hh
Other Total Individuals 63
(70) 1000
Total Taxa 17 (18)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Veliidae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-5 STATION NAME Lick Run LAT N 39deg 12752rsquo (upstream) LONG W 078deg 05685rsquo (upstream) LAT N 39deg 12784rsquo (downstream) LONG W 078deg 05631rsquo (downstream) FORM COMPILED BY Brandon Millholland W Bousquet RIVER BASIN Potomac River INVESTIGATORS J Pangle S Robertson W Bousquet JTovar M Dahl
Collection date 29 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Gomphidae 1 15 I BVW PR 3 BU Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Leptophlebiidae 11 167 A LBM BVW CG 2 CR Plecoptera (stoneflies) Perlidae 10 152 A LBM BVW PR 1 CR Perlodiae 1 15 A LBM BVW PR 2 CR Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 5 76 A LBM BVW SC 4 CG Elmidae 2 30 I LBM BVW SC 4 CG Diptera (true flies) (Culicidae) (3) - P LMB BVW ( ) Chironomidae 23 348 I LBM BVW CG 6 BU Simuliidae 13 197 I LBM BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
ii
jj
Other Total Individuals 66
(69) 1000
Total Taxa 7 (8)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Culicidae (Diptera) pupae not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl J Pangle S Robertson W Bousquet B Millholland J Tovar H Kepple
Collection date 30 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 1 12 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Asellidae 114 965 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Plecoptera (stoneflies) Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 2 23 A MRD BVW SC 4 CG Diptera (true flies)
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
kk
ll
Other Total Individuals 117 1000 Total Taxa 3
Notes Subsample taken 29 October 2009 All taxonomic determinations by MRD were confirmed by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson K McCann A Fisher H Kepple A Barrett
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 2 17 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Sphaeriidae 2 17 I MRD BVW CF 8 BU Isopoda (isopods) Asellidae 99 853 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Calopterygidae 2 17 A MRD BVW PR 7 CL Ephemeroptera (mayflies) Leptophlebiidae 2 17 I MRD BVW CG 2 CR Heptageniidae 4 34 A MRD BVW SC 3 CG Plecoptera (stoneflies) Perlidae 1 09 A MRD BVW PR 1 CR Trichoptera (caddisflies) Hemiptera (true bugs) (Gerridae) (1) - A MRD BVW ( ) Corixidae 1 09 A MRD BVW PR 5 CR Megaloptera (dobsonflies etc) Coleoptera (beetles) Dryopidae 1 09 A MRD BVW SC 5 CG Diptera (true flies) Tipulidae 2 17 I BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
mm
nn
Other Total Individuals 116
(117) 999
Total Taxa 10 (11)
Notes Subsample taken 6 November 2008 Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009 Gerridae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
- Disclaimer ii
- Title Page iii
- Acknowledgements v
- Table of Contents vii
- SECTION 2 RESULTS DATA TABLES 7
- OVERVIEW AND FINDINGS
- Introduction
- RESULTS DATA TABLES
- REFERENCES
- FIELD DATA SHEETS
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- SubstrateType
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- WATER QUALITY ANALYSIS
- THROUGH THE VIRGINIA
- STREAM CONDITION INDEX (VSCI)
- LABORATORY BENCH SHEETS
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
ff
Other Total Individuals 72 1001 Total Taxa 7
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-4 STATION NAME Unnamed Tributary LAT (upstream) N 39deg 13035rsquo LONG (upstream) W 078deg 05129rsquo LAT (downstream) N 39deg 13000rsquo LONG (downstream) W 078deg 5086rsquo FORM COMPILED BY Andrew G M Fisher W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson A Fisher H Kepple
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Flatworms (Turbellaria) Worms (Oligochaeta) Lumbriculidae 2 32 A AF BVW CG 8 BU Leeches (Hirudinea) Snails (Gastropoda) Lymnaeidae 1 16 A BVW CG 9 GN Clams (Bivalvia) Isopods (Isopoda) Scuds (Amphipoda) Crayfish Shrimp (Decapoda) Cambaridae 1 16 A AF BVW GN 5 GN Water Mites (Hydracarina) Dragonflies (Odonata Anisoptera) Damselflies (Odonata Zygoptera) Calopterygidae 4 63 I BVW PR 7 CL Mayflies (Ephemeroptera) Tricorythidae 1 16 I BVW CG 5 SP Stoneflies (Plecoptera) Caddisflies (Trichoptera) Phryganeidae 4 63 I AF BVW SH 4 CR Hydropsychidae 6 95 I AF BVW CF 6 CG I True Bugs (Hemiptera) Corixidae 8 127 I AF BVW MP 9 DV (Veliidae ) (7) - I AF BVW ( ) Dobsonflies (Megaloptera) Sialidae 3 48 I AF BVW PR 7 SP Corydalidae 2 32 I AF BVW PR 5 CR Beetles (Coleoptera) Haliplidae 1 16 A AF BVW MP 8 GN Dysticidae 7 111 I AF BVW PR 6 GN Hydrophilidae 1 16 A AF BVW PR 8 GN Elmidae 3 48 I BVW SC 4 CG True Flies (Diptera) Chironomidae 8 127 I AF BVW CG 6 BU Simuliidae 7 111 I AF BVW CF 6 CG Tipulidae 4 63 I AF BVW SH 4 GU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
gg
hh
Other Total Individuals 63
(70) 1000
Total Taxa 17 (18)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Veliidae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-5 STATION NAME Lick Run LAT N 39deg 12752rsquo (upstream) LONG W 078deg 05685rsquo (upstream) LAT N 39deg 12784rsquo (downstream) LONG W 078deg 05631rsquo (downstream) FORM COMPILED BY Brandon Millholland W Bousquet RIVER BASIN Potomac River INVESTIGATORS J Pangle S Robertson W Bousquet JTovar M Dahl
Collection date 29 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Gomphidae 1 15 I BVW PR 3 BU Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Leptophlebiidae 11 167 A LBM BVW CG 2 CR Plecoptera (stoneflies) Perlidae 10 152 A LBM BVW PR 1 CR Perlodiae 1 15 A LBM BVW PR 2 CR Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 5 76 A LBM BVW SC 4 CG Elmidae 2 30 I LBM BVW SC 4 CG Diptera (true flies) (Culicidae) (3) - P LMB BVW ( ) Chironomidae 23 348 I LBM BVW CG 6 BU Simuliidae 13 197 I LBM BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
ii
jj
Other Total Individuals 66
(69) 1000
Total Taxa 7 (8)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Culicidae (Diptera) pupae not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl J Pangle S Robertson W Bousquet B Millholland J Tovar H Kepple
Collection date 30 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 1 12 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Asellidae 114 965 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Plecoptera (stoneflies) Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 2 23 A MRD BVW SC 4 CG Diptera (true flies)
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
kk
ll
Other Total Individuals 117 1000 Total Taxa 3
Notes Subsample taken 29 October 2009 All taxonomic determinations by MRD were confirmed by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson K McCann A Fisher H Kepple A Barrett
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 2 17 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Sphaeriidae 2 17 I MRD BVW CF 8 BU Isopoda (isopods) Asellidae 99 853 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Calopterygidae 2 17 A MRD BVW PR 7 CL Ephemeroptera (mayflies) Leptophlebiidae 2 17 I MRD BVW CG 2 CR Heptageniidae 4 34 A MRD BVW SC 3 CG Plecoptera (stoneflies) Perlidae 1 09 A MRD BVW PR 1 CR Trichoptera (caddisflies) Hemiptera (true bugs) (Gerridae) (1) - A MRD BVW ( ) Corixidae 1 09 A MRD BVW PR 5 CR Megaloptera (dobsonflies etc) Coleoptera (beetles) Dryopidae 1 09 A MRD BVW SC 5 CG Diptera (true flies) Tipulidae 2 17 I BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
mm
nn
Other Total Individuals 116
(117) 999
Total Taxa 10 (11)
Notes Subsample taken 6 November 2008 Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009 Gerridae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
- Disclaimer ii
- Title Page iii
- Acknowledgements v
- Table of Contents vii
- SECTION 2 RESULTS DATA TABLES 7
- OVERVIEW AND FINDINGS
- Introduction
- RESULTS DATA TABLES
- REFERENCES
- FIELD DATA SHEETS
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- SubstrateType
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- WATER QUALITY ANALYSIS
- THROUGH THE VIRGINIA
- STREAM CONDITION INDEX (VSCI)
- LABORATORY BENCH SHEETS
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-4 STATION NAME Unnamed Tributary LAT (upstream) N 39deg 13035rsquo LONG (upstream) W 078deg 05129rsquo LAT (downstream) N 39deg 13000rsquo LONG (downstream) W 078deg 5086rsquo FORM COMPILED BY Andrew G M Fisher W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson A Fisher H Kepple
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Flatworms (Turbellaria) Worms (Oligochaeta) Lumbriculidae 2 32 A AF BVW CG 8 BU Leeches (Hirudinea) Snails (Gastropoda) Lymnaeidae 1 16 A BVW CG 9 GN Clams (Bivalvia) Isopods (Isopoda) Scuds (Amphipoda) Crayfish Shrimp (Decapoda) Cambaridae 1 16 A AF BVW GN 5 GN Water Mites (Hydracarina) Dragonflies (Odonata Anisoptera) Damselflies (Odonata Zygoptera) Calopterygidae 4 63 I BVW PR 7 CL Mayflies (Ephemeroptera) Tricorythidae 1 16 I BVW CG 5 SP Stoneflies (Plecoptera) Caddisflies (Trichoptera) Phryganeidae 4 63 I AF BVW SH 4 CR Hydropsychidae 6 95 I AF BVW CF 6 CG I True Bugs (Hemiptera) Corixidae 8 127 I AF BVW MP 9 DV (Veliidae ) (7) - I AF BVW ( ) Dobsonflies (Megaloptera) Sialidae 3 48 I AF BVW PR 7 SP Corydalidae 2 32 I AF BVW PR 5 CR Beetles (Coleoptera) Haliplidae 1 16 A AF BVW MP 8 GN Dysticidae 7 111 I AF BVW PR 6 GN Hydrophilidae 1 16 A AF BVW PR 8 GN Elmidae 3 48 I BVW SC 4 CG True Flies (Diptera) Chironomidae 8 127 I AF BVW CG 6 BU Simuliidae 7 111 I AF BVW CF 6 CG Tipulidae 4 63 I AF BVW SH 4 GU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
gg
hh
Other Total Individuals 63
(70) 1000
Total Taxa 17 (18)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Veliidae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-5 STATION NAME Lick Run LAT N 39deg 12752rsquo (upstream) LONG W 078deg 05685rsquo (upstream) LAT N 39deg 12784rsquo (downstream) LONG W 078deg 05631rsquo (downstream) FORM COMPILED BY Brandon Millholland W Bousquet RIVER BASIN Potomac River INVESTIGATORS J Pangle S Robertson W Bousquet JTovar M Dahl
Collection date 29 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Gomphidae 1 15 I BVW PR 3 BU Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Leptophlebiidae 11 167 A LBM BVW CG 2 CR Plecoptera (stoneflies) Perlidae 10 152 A LBM BVW PR 1 CR Perlodiae 1 15 A LBM BVW PR 2 CR Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 5 76 A LBM BVW SC 4 CG Elmidae 2 30 I LBM BVW SC 4 CG Diptera (true flies) (Culicidae) (3) - P LMB BVW ( ) Chironomidae 23 348 I LBM BVW CG 6 BU Simuliidae 13 197 I LBM BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
ii
jj
Other Total Individuals 66
(69) 1000
Total Taxa 7 (8)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Culicidae (Diptera) pupae not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl J Pangle S Robertson W Bousquet B Millholland J Tovar H Kepple
Collection date 30 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 1 12 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Asellidae 114 965 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Plecoptera (stoneflies) Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 2 23 A MRD BVW SC 4 CG Diptera (true flies)
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
kk
ll
Other Total Individuals 117 1000 Total Taxa 3
Notes Subsample taken 29 October 2009 All taxonomic determinations by MRD were confirmed by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson K McCann A Fisher H Kepple A Barrett
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 2 17 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Sphaeriidae 2 17 I MRD BVW CF 8 BU Isopoda (isopods) Asellidae 99 853 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Calopterygidae 2 17 A MRD BVW PR 7 CL Ephemeroptera (mayflies) Leptophlebiidae 2 17 I MRD BVW CG 2 CR Heptageniidae 4 34 A MRD BVW SC 3 CG Plecoptera (stoneflies) Perlidae 1 09 A MRD BVW PR 1 CR Trichoptera (caddisflies) Hemiptera (true bugs) (Gerridae) (1) - A MRD BVW ( ) Corixidae 1 09 A MRD BVW PR 5 CR Megaloptera (dobsonflies etc) Coleoptera (beetles) Dryopidae 1 09 A MRD BVW SC 5 CG Diptera (true flies) Tipulidae 2 17 I BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
mm
nn
Other Total Individuals 116
(117) 999
Total Taxa 10 (11)
Notes Subsample taken 6 November 2008 Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009 Gerridae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
- Disclaimer ii
- Title Page iii
- Acknowledgements v
- Table of Contents vii
- SECTION 2 RESULTS DATA TABLES 7
- OVERVIEW AND FINDINGS
- Introduction
- RESULTS DATA TABLES
- REFERENCES
- FIELD DATA SHEETS
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- SubstrateType
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- WATER QUALITY ANALYSIS
- THROUGH THE VIRGINIA
- STREAM CONDITION INDEX (VSCI)
- LABORATORY BENCH SHEETS
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
hh
Other Total Individuals 63
(70) 1000
Total Taxa 17 (18)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Veliidae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-5 STATION NAME Lick Run LAT N 39deg 12752rsquo (upstream) LONG W 078deg 05685rsquo (upstream) LAT N 39deg 12784rsquo (downstream) LONG W 078deg 05631rsquo (downstream) FORM COMPILED BY Brandon Millholland W Bousquet RIVER BASIN Potomac River INVESTIGATORS J Pangle S Robertson W Bousquet JTovar M Dahl
Collection date 29 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Gomphidae 1 15 I BVW PR 3 BU Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Leptophlebiidae 11 167 A LBM BVW CG 2 CR Plecoptera (stoneflies) Perlidae 10 152 A LBM BVW PR 1 CR Perlodiae 1 15 A LBM BVW PR 2 CR Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 5 76 A LBM BVW SC 4 CG Elmidae 2 30 I LBM BVW SC 4 CG Diptera (true flies) (Culicidae) (3) - P LMB BVW ( ) Chironomidae 23 348 I LBM BVW CG 6 BU Simuliidae 13 197 I LBM BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
ii
jj
Other Total Individuals 66
(69) 1000
Total Taxa 7 (8)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Culicidae (Diptera) pupae not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl J Pangle S Robertson W Bousquet B Millholland J Tovar H Kepple
Collection date 30 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 1 12 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Asellidae 114 965 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Plecoptera (stoneflies) Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 2 23 A MRD BVW SC 4 CG Diptera (true flies)
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
kk
ll
Other Total Individuals 117 1000 Total Taxa 3
Notes Subsample taken 29 October 2009 All taxonomic determinations by MRD were confirmed by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson K McCann A Fisher H Kepple A Barrett
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 2 17 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Sphaeriidae 2 17 I MRD BVW CF 8 BU Isopoda (isopods) Asellidae 99 853 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Calopterygidae 2 17 A MRD BVW PR 7 CL Ephemeroptera (mayflies) Leptophlebiidae 2 17 I MRD BVW CG 2 CR Heptageniidae 4 34 A MRD BVW SC 3 CG Plecoptera (stoneflies) Perlidae 1 09 A MRD BVW PR 1 CR Trichoptera (caddisflies) Hemiptera (true bugs) (Gerridae) (1) - A MRD BVW ( ) Corixidae 1 09 A MRD BVW PR 5 CR Megaloptera (dobsonflies etc) Coleoptera (beetles) Dryopidae 1 09 A MRD BVW SC 5 CG Diptera (true flies) Tipulidae 2 17 I BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
mm
nn
Other Total Individuals 116
(117) 999
Total Taxa 10 (11)
Notes Subsample taken 6 November 2008 Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009 Gerridae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
- Disclaimer ii
- Title Page iii
- Acknowledgements v
- Table of Contents vii
- SECTION 2 RESULTS DATA TABLES 7
- OVERVIEW AND FINDINGS
- Introduction
- RESULTS DATA TABLES
- REFERENCES
- FIELD DATA SHEETS
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- SubstrateType
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- WATER QUALITY ANALYSIS
- THROUGH THE VIRGINIA
- STREAM CONDITION INDEX (VSCI)
- LABORATORY BENCH SHEETS
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co VA STATION HLR-5 STATION NAME Lick Run LAT N 39deg 12752rsquo (upstream) LONG W 078deg 05685rsquo (upstream) LAT N 39deg 12784rsquo (downstream) LONG W 078deg 05631rsquo (downstream) FORM COMPILED BY Brandon Millholland W Bousquet RIVER BASIN Potomac River INVESTIGATORS J Pangle S Robertson W Bousquet JTovar M Dahl
Collection date 29 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Gomphidae 1 15 I BVW PR 3 BU Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Leptophlebiidae 11 167 A LBM BVW CG 2 CR Plecoptera (stoneflies) Perlidae 10 152 A LBM BVW PR 1 CR Perlodiae 1 15 A LBM BVW PR 2 CR Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 5 76 A LBM BVW SC 4 CG Elmidae 2 30 I LBM BVW SC 4 CG Diptera (true flies) (Culicidae) (3) - P LMB BVW ( ) Chironomidae 23 348 I LBM BVW CG 6 BU Simuliidae 13 197 I LBM BVW CF 6 CG
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
ii
jj
Other Total Individuals 66
(69) 1000
Total Taxa 7 (8)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Culicidae (Diptera) pupae not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl J Pangle S Robertson W Bousquet B Millholland J Tovar H Kepple
Collection date 30 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 1 12 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Asellidae 114 965 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Plecoptera (stoneflies) Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 2 23 A MRD BVW SC 4 CG Diptera (true flies)
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
kk
ll
Other Total Individuals 117 1000 Total Taxa 3
Notes Subsample taken 29 October 2009 All taxonomic determinations by MRD were confirmed by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson K McCann A Fisher H Kepple A Barrett
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 2 17 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Sphaeriidae 2 17 I MRD BVW CF 8 BU Isopoda (isopods) Asellidae 99 853 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Calopterygidae 2 17 A MRD BVW PR 7 CL Ephemeroptera (mayflies) Leptophlebiidae 2 17 I MRD BVW CG 2 CR Heptageniidae 4 34 A MRD BVW SC 3 CG Plecoptera (stoneflies) Perlidae 1 09 A MRD BVW PR 1 CR Trichoptera (caddisflies) Hemiptera (true bugs) (Gerridae) (1) - A MRD BVW ( ) Corixidae 1 09 A MRD BVW PR 5 CR Megaloptera (dobsonflies etc) Coleoptera (beetles) Dryopidae 1 09 A MRD BVW SC 5 CG Diptera (true flies) Tipulidae 2 17 I BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
mm
nn
Other Total Individuals 116
(117) 999
Total Taxa 10 (11)
Notes Subsample taken 6 November 2008 Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009 Gerridae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
- Disclaimer ii
- Title Page iii
- Acknowledgements v
- Table of Contents vii
- SECTION 2 RESULTS DATA TABLES 7
- OVERVIEW AND FINDINGS
- Introduction
- RESULTS DATA TABLES
- REFERENCES
- FIELD DATA SHEETS
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- SubstrateType
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- WATER QUALITY ANALYSIS
- THROUGH THE VIRGINIA
- STREAM CONDITION INDEX (VSCI)
- LABORATORY BENCH SHEETS
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
jj
Other Total Individuals 66
(69) 1000
Total Taxa 7 (8)
Notes Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 1 April 2009 Culicidae (Diptera) pupae not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl J Pangle S Robertson W Bousquet B Millholland J Tovar H Kepple
Collection date 30 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 1 12 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Asellidae 114 965 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Plecoptera (stoneflies) Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 2 23 A MRD BVW SC 4 CG Diptera (true flies)
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
kk
ll
Other Total Individuals 117 1000 Total Taxa 3
Notes Subsample taken 29 October 2009 All taxonomic determinations by MRD were confirmed by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson K McCann A Fisher H Kepple A Barrett
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 2 17 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Sphaeriidae 2 17 I MRD BVW CF 8 BU Isopoda (isopods) Asellidae 99 853 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Calopterygidae 2 17 A MRD BVW PR 7 CL Ephemeroptera (mayflies) Leptophlebiidae 2 17 I MRD BVW CG 2 CR Heptageniidae 4 34 A MRD BVW SC 3 CG Plecoptera (stoneflies) Perlidae 1 09 A MRD BVW PR 1 CR Trichoptera (caddisflies) Hemiptera (true bugs) (Gerridae) (1) - A MRD BVW ( ) Corixidae 1 09 A MRD BVW PR 5 CR Megaloptera (dobsonflies etc) Coleoptera (beetles) Dryopidae 1 09 A MRD BVW SC 5 CG Diptera (true flies) Tipulidae 2 17 I BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
mm
nn
Other Total Individuals 116
(117) 999
Total Taxa 10 (11)
Notes Subsample taken 6 November 2008 Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009 Gerridae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
- Disclaimer ii
- Title Page iii
- Acknowledgements v
- Table of Contents vii
- SECTION 2 RESULTS DATA TABLES 7
- OVERVIEW AND FINDINGS
- Introduction
- RESULTS DATA TABLES
- REFERENCES
- FIELD DATA SHEETS
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- SubstrateType
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- WATER QUALITY ANALYSIS
- THROUGH THE VIRGINIA
- STREAM CONDITION INDEX (VSCI)
- LABORATORY BENCH SHEETS
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl J Pangle S Robertson W Bousquet B Millholland J Tovar H Kepple
Collection date 30 May 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 1 12 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Isopoda (isopods) Asellidae 114 965 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Ephemeroptera (mayflies) Plecoptera (stoneflies) Trichoptera (caddisflies) Hemiptera (true bugs) Megaloptera (dobsonflies etc) Coleoptera (beetles) Elmidae 2 23 A MRD BVW SC 4 CG Diptera (true flies)
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
kk
ll
Other Total Individuals 117 1000 Total Taxa 3
Notes Subsample taken 29 October 2009 All taxonomic determinations by MRD were confirmed by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson K McCann A Fisher H Kepple A Barrett
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 2 17 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Sphaeriidae 2 17 I MRD BVW CF 8 BU Isopoda (isopods) Asellidae 99 853 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Calopterygidae 2 17 A MRD BVW PR 7 CL Ephemeroptera (mayflies) Leptophlebiidae 2 17 I MRD BVW CG 2 CR Heptageniidae 4 34 A MRD BVW SC 3 CG Plecoptera (stoneflies) Perlidae 1 09 A MRD BVW PR 1 CR Trichoptera (caddisflies) Hemiptera (true bugs) (Gerridae) (1) - A MRD BVW ( ) Corixidae 1 09 A MRD BVW PR 5 CR Megaloptera (dobsonflies etc) Coleoptera (beetles) Dryopidae 1 09 A MRD BVW SC 5 CG Diptera (true flies) Tipulidae 2 17 I BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
mm
nn
Other Total Individuals 116
(117) 999
Total Taxa 10 (11)
Notes Subsample taken 6 November 2008 Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009 Gerridae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
- Disclaimer ii
- Title Page iii
- Acknowledgements v
- Table of Contents vii
- SECTION 2 RESULTS DATA TABLES 7
- OVERVIEW AND FINDINGS
- Introduction
- RESULTS DATA TABLES
- REFERENCES
- FIELD DATA SHEETS
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- SubstrateType
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- WATER QUALITY ANALYSIS
- THROUGH THE VIRGINIA
- STREAM CONDITION INDEX (VSCI)
- LABORATORY BENCH SHEETS
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
ll
Other Total Individuals 117 1000 Total Taxa 3
Notes Subsample taken 29 October 2009 All taxonomic determinations by MRD were confirmed by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson K McCann A Fisher H Kepple A Barrett
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 2 17 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Sphaeriidae 2 17 I MRD BVW CF 8 BU Isopoda (isopods) Asellidae 99 853 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Calopterygidae 2 17 A MRD BVW PR 7 CL Ephemeroptera (mayflies) Leptophlebiidae 2 17 I MRD BVW CG 2 CR Heptageniidae 4 34 A MRD BVW SC 3 CG Plecoptera (stoneflies) Perlidae 1 09 A MRD BVW PR 1 CR Trichoptera (caddisflies) Hemiptera (true bugs) (Gerridae) (1) - A MRD BVW ( ) Corixidae 1 09 A MRD BVW PR 5 CR Megaloptera (dobsonflies etc) Coleoptera (beetles) Dryopidae 1 09 A MRD BVW SC 5 CG Diptera (true flies) Tipulidae 2 17 I BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
mm
nn
Other Total Individuals 116
(117) 999
Total Taxa 10 (11)
Notes Subsample taken 6 November 2008 Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009 Gerridae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
- Disclaimer ii
- Title Page iii
- Acknowledgements v
- Table of Contents vii
- SECTION 2 RESULTS DATA TABLES 7
- OVERVIEW AND FINDINGS
- Introduction
- RESULTS DATA TABLES
- REFERENCES
- FIELD DATA SHEETS
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- SubstrateType
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- WATER QUALITY ANALYSIS
- THROUGH THE VIRGINIA
- STREAM CONDITION INDEX (VSCI)
- LABORATORY BENCH SHEETS
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
Environmental Studies Department Shenandoah University BENTHIC MACROINVERTEBRATE LABORATORY BENCH SHEET AND VSCI WORKSHEET
STREAM NAME Hiatt-Lick Run LOCATION (citycounty state) Stephenson Frederick Co Virginia STATION HLR-6 STATION NAME McCann Farm LAT N 39deg 13076rsquo (upstream) LONG W 078deg 06539rsquo (upstream) LAT N 39deg 13063rsquo (downstream) LONG W 078deg 06473rsquo (downstream) FORM COMPILED BY Marie Dahl W Bousquet RIVER BASIN Potomac River INVESTIGATORS M Dahl S Robertson K McCann A Fisher H Kepple A Barrett
Collection date 10 Oct 2008 Sheet completion date 10 Dec 2008 rev 17 April 2009
PROJECT NAME Hiatt-Lick Run BMI Water Quality Project -- 2008
Order Taxon (usually family) Total Relative
No () Life Stage
Taxonomistrsquos Initials
VSCI Classification FG TV HAB
Turbellaria (flatworms) Turbellaria 2 17 A MRD BVW CG 8 SP Oligochaeta (worms) Hirudinea (leeches) Gastropoda (snails) Bivalvia (clams) Sphaeriidae 2 17 I MRD BVW CF 8 BU Isopoda (isopods) Asellidae 99 853 I-A MRD BVW CG 8 SP Amphipoda (scuds) Decapoda (crayfish shrimp) Hydracarina (water mites) Odonata Anisoptera (dragonflies) Odonata Zygoptera (damselflies) Calopterygidae 2 17 A MRD BVW PR 7 CL Ephemeroptera (mayflies) Leptophlebiidae 2 17 I MRD BVW CG 2 CR Heptageniidae 4 34 A MRD BVW SC 3 CG Plecoptera (stoneflies) Perlidae 1 09 A MRD BVW PR 1 CR Trichoptera (caddisflies) Hemiptera (true bugs) (Gerridae) (1) - A MRD BVW ( ) Corixidae 1 09 A MRD BVW PR 5 CR Megaloptera (dobsonflies etc) Coleoptera (beetles) Dryopidae 1 09 A MRD BVW SC 5 CG Diptera (true flies) Tipulidae 2 17 I BVW SH 4 BU
LS = Life Stage I = immature A = adult (P = pupa not counted in total) Subsampled X YES NO
mm
nn
Other Total Individuals 116
(117) 999
Total Taxa 10 (11)
Notes Subsample taken 6 November 2008 Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009 Gerridae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
- Disclaimer ii
- Title Page iii
- Acknowledgements v
- Table of Contents vii
- SECTION 2 RESULTS DATA TABLES 7
- OVERVIEW AND FINDINGS
- Introduction
- RESULTS DATA TABLES
- REFERENCES
- FIELD DATA SHEETS
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- SubstrateType
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- WATER QUALITY ANALYSIS
- THROUGH THE VIRGINIA
- STREAM CONDITION INDEX (VSCI)
- LABORATORY BENCH SHEETS
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
nn
Other Total Individuals 116
(117) 999
Total Taxa 10 (11)
Notes Subsample taken 6 November 2008 Revised as per taxonomic review by William J (Billy) Van Wart Regional Biologist Virginia Department of Environmental Quality on 31 March 2009 Gerridae (Hemiptera) not counted in VSCI
This form is based on the Benthic Macroinvertebrate Laboratory Bench Sheet developed by the Environmental Protection Agency for use in the Rapid Bioassessment Protocols for Benthic Macroinvertebrates 10 December 2008
- Disclaimer ii
- Title Page iii
- Acknowledgements v
- Table of Contents vii
- SECTION 2 RESULTS DATA TABLES 7
- OVERVIEW AND FINDINGS
- Introduction
- RESULTS DATA TABLES
- REFERENCES
- FIELD DATA SHEETS
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- SubstrateType
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- INORGANIC SUBSTRATE COMPONENTS
- ORGANIC SUBSTRATE COMPONENTS
- Substrate
- Diameter
- Substrate
- Characteristic
- WATER QUALITY ANALYSIS
- THROUGH THE VIRGINIA
- STREAM CONDITION INDEX (VSCI)
- LABORATORY BENCH SHEETS
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Flatworms (Turbellaria)
- Worms (Oligochaeta)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
- Taxon (usually family)
- Total
- Turbellaria (flatworms)
- Oligochaeta (worms)
- Total Individuals
- Total Taxa
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