A&P 1 - Lab #2 Microscopy Cell Structure Mitosis.
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A&P 1 - Lab #2
Microscopy
Cell Structure
Mitosis
Rotatable Head
Ocular Lens
Objective Lens
Arm
Illuminator
Condenser
Stage
Base
Course Adjustment Knob
Fine Adjustment Knob
Mechanical Stage
Mechanical Stage Control
Magnification
• Total magnification is achieved by multiplying the power of the ocular lens by the power of the objective lens
Total Magnification = Ocular Lens X Objective Lens
Objective Lens
• Red Band = Low Power = 4
4(obj lens) X 10(ocular lens) = 40X
• Yellow Band = Medium Power = 10Yellow Band = Medium Power = 10
10(obj lens) X 10(ocular lens) = 100X10(obj lens) X 10(ocular lens) = 100X
• Blue Band = High Power = 100Blue Band = High Power = 100
40(obj lens) X 10(ocular lens) = 400X40(obj lens) X 10(ocular lens) = 400X
• White Band = Oil Immersion
Follow the Rules!
• ALWAYS begin on LOW POWER– Once you have the specimen in focus– Move to medium power– Refocus, then you can move to high power
• Once you move off low power, you can NEVER, NEVER, NEVER use the coarse adjustment
Orientation of Specimen
Specimen is flipped horizontally & vertically
Depth of Field
Preparing a Wet Mount
Cover Slip 45O
Slowly LowerCover Slip
Air Bubbles - Try Again!
Types of Cells
• There are 2 types of cells:
Prokaryotic Eukaryotic (pro- before) (eu- true)
-kary = nucleus
Prokaryotic Cells
Prokaryotic Cells
Prokaryotic cells -lack a membrane-bound nucleus.-genetic material is present in the nucleoid
ALL prokaryotic cells are bacteria, and ALL bacteria are prokaryotic
The only place you will find prokaryotic cells in is the KINGDOM BACTERIA AND ARCHEBACTERIA
Eukaryotic Cells
Epithelial - Cheek Cells
The Cell Cycle
• Interphase
• Mitosis– Prophase - prepare– Metaphase - middle– Anaphase - apart– Telophase - two
• Cytokinesis cyto- = cell -kinesis = to move
The Cell Cycle
Interphase
Mitosis - Prophase
Mitosis - Metaphase
Mitosis - Metaphase
Mitosis - Anaphase
Mitosis - Anaphase
Mitosis - Telophase
Mitosis - Telophase/Cytokinesis
Practice Identifying the Stages of Mitosis
• Mitosis
Select the link below (you must be in “slide show” mode)If you are unable to view a slide show, you can go toBookmarks on Ocean Cruiser. The link is found there.
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