226 PHT Final Spots. Gram’s stain Results: Shape: Cocci Arrangment: irregular clusters Colour: Violet Gram’s reaction: Gram’s +ve Name of microorganism:

226 PHT Final Spots

Gram’s stain

Results:

Shape: Shape: CocciCocci

Arrangment: Arrangment: irregularirregular clustersclusters

Colour: Colour: VioletViolet

Gram’s reaction: Gram’s reaction: Gram’sGram’s +ve+ve

Name of microorganism: Name of microorganism:

StaphylococciStaphylococci

Results:

Shape: Shape: OvalOval

Arrangment: Arrangment: SingleSingle

Colour: Colour: VioletViolet

Gram’s reaction: Gram’s reaction: Gram’s +veGram’s +ve

Name of microorganism: Name of microorganism:

Candida Candida

Results:

Shape: Shape: BacilliBacilli

Arrangment: Arrangment: ChainsChains

Colour: Colour: VioletViolet

Gram’s reaction: Gram’s reaction: Gram’s +veGram’s +ve

Name of microorganism: Name of microorganism:

Bacillus Bacillus

Results:

Shape: Shape: RodsRods

Arrangment: Arrangment: SingleSingle

Colour: Colour: redred

Gram’s reaction: Gram’s reaction: Gram’s –veGram’s –ve

Name of microorganism: Name of microorganism:

Gram negative Gram negative bacillibacilli

Negative stainingNegative staining

Candida

Negative staining

Staphylococci

Negative staining of Bacillus

Results

Type of Staining: Acid fast stain

Shape: beaded bacilli

Arrangement: Tree shaped

Colour: red

Name of microorganism: M.phelei

Spore Stain ofBacillus subtilis

Type of Staining: Spore stain

Shape: bacilli

Arrangement: Chains

Colour of spores: green

Colour of vegetative cells: red

Name of microorganism:

B. subtilis

Nutrient agar plateNutrient agar plate Nutrient agar slantNutrient agar slant

Nutrient brothNutrient broth

Simple media

Enriched media

Serum agar blood agar

non-hemolytic Sterptococci.

β-hemolytic Sterptococci.α-hemolytic Sterptococci.

Hemolysis of blood agar

Selective medium

L-J

Selective medium

Cetremide agar of pseudomonas

Growth with yellow colour around the colonies S.aureus

Growth without change in the colour of the medium S.epidermidis

1-Mannitol salt agar1-Mannitol salt agar

Selective and differential media:Selective and differential media:

Lactose fermenter Pink colonies

Lactose non-fermenter Pale colonies

2-MacConkey agar2-MacConkey agar

Cooked meat

Anaerobic Jar

Results:Positive test: formation of visible clot.

Coagulase +ve

S.aureus

Coagulase -ve

S.epidermidis

1- Coagulase Test:1- Coagulase Test:

2-Oxidase test:

Results:+ve Test: Appearance of purple colour within 1-2 min.

purple colour

+ve test

No colour

-ve test

Results:

+ve

-ve

3- Urease Test:3- Urease Test:

1. Sugar fermentation:1. Sugar fermentation:

Glucose Maltose Sucrose Glucose Maltose Sucrose

Sugar fermentation can be indicated by change of color of the medium from red to yellow.

+ve - ve+ve +ve +ve +ve

Results:

Butt:

Slant:

H2S Production:

acidic (yellow)

acidic (yellow)

-ve

acidic (yellow)

alkaline (red)

-ve

acidic (yellow)

alkaline (red)

+ve

2- 2- Triple Sugar Iron (TSI) agar:Triple Sugar Iron (TSI) agar:

O-/F- O+/F+ O+/F- O-/F+

Results:

Fermentative OxidativeNon Saccharolytic

Positive Test:

3-O/F Test (Oxidation Fermentation Test)

Positive Test:

4-Sensitive Oxidation Fermentation (O/F)Test:

O-/F- O+/F+ O+/F- O-/F+

Results:

Fermentative OxidativeNon Saccharolytic

Results:

+ve-ve

1- Indole test

Methods for determination of MIC

E-test:-

Elek’s Toxigenicity Test: