2-AB (2-aminobenzamide) Labeled Glucose Homopolymer (GHP ... · Certificate of Analysis LudgerPureTM 2AB Labeled Glucose Homopolymer Cat. # : CAB-GHP-30 Lot # : A3AO-01 Size : approx.
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2-AB Labeled Glucose Homopolymer Ladder : Cat. No. CAB-GHP-x © Ludger Ltd, 2005 Page 1
2-AB (2-aminobenzamide) Labeled Glucose Homopolymer (GHP) Ladder
Cat. No. CAB-GHP-x (where x denotes pack size)
Structure [Glcα1-6]n Glc-2AB
Synonyms : 2-AB labeled dextran hydrolysate ladder
Description: 2-aminobenzamide labeled glucose homopolymer ladder for use as a calibration
standard for HPLC and MS analysis of glycans. The ladder contains glucose
homopolymer species from degree of polymerization (dp) 1 to up to dp 22 (i.e. the
glucose monomer GU1-2AB to GU22-2AB).
Preparation : Glucose homopolymer ladder was obtained by controlled acid hydrolysis of dextran
then labeled by reductive amination at the reducing termini with the fluorescent glycan
tag 2-aminobenzamide (2-AB).
Form: Dry. Dried by centrifugal evaporation from an aqueous solution.
Purity: > 90% pure as assessed by HPLC.
Use: See Certificate of Analysis for recommended sample dilution and injection volume.
Storage: Refrigerate (-20oC) both before and after dissolution. This product is stable for at least
5 years as supplied.
Shipping: The product can be shipped at ambient when dry. After dissolution, ship on dry ice.
Handling: Allow the unopened vial to reach ambient temperature and tap unopened on a solid
surface to ensure that most of the lyophilized material is at the bottom of the vial.
Gently remove the cap, add the desired volume of reconstitution medium, re-cap and
mix thoroughly to bring all the oligosaccharide into solution. We recommend
analytical grade pure water as the reconstitution medium. For maximal recovery of
oligosaccharide, ensure that the cap lining is also rinsed and centrifuge the
2-AB Labeled Glucose Homopolymer Ladder : Cat. No. CAB-GHP-x © Ludger Ltd, 2005 Page 2
reconstituted vial briefly before use. Ensure that any glass, plastic ware or solvents
used are free of glycosidases and environmental carbohydrates.
Safety: This product is non-hazardous and has been purified from natural sources certified to
be free of all hazardous material including pathogenic biological agents.
For research use only. Not for human or drug use
Warranties and liabilities
Ludger warrants that the above product conforms to the attached analytical documents. Should the product
fail for reasons other than through misuse Ludger will, at its option, replace free of charge or refund the
purchase price. This warranty is exclusive and Ludger makes no other warrants, expressed or implied,
including any implied conditions or warranties of merchantability or fitness for any particular purpose.
Ludger shall not be liable for any incidental, consequential or contingent damages.
This product is intended for in vitro research only.
Document # 'CAB-GHP-Guide', revision 1.1
Certificate of Analysis
LudgerPureTM 2AB Labeled Glucose Homopolymer
Cat. # : CAB-GHP-30 Lot # : A3AO-01 Size : approx. 1 nmol of G1
Purity: > 95% pure as assessed by HPLC (see Fig 1).
0 10-200
500
1,000
1,500
2,000 LudgerSepN1-A51Q-01-CAB-GHP-30-A3AO-01 FluorescencemV
10.5
1
13.2
7
Figure 1 : LudgerS
No. A3AO-01).
HPLC Running C
Column: LudgerSep N
Solvent A: 100 % aceton
Gradient: 0-7
75-
80-
83-
85-
Detector: Jasco FP920
Sample Dilution
Redissolve CAB-GHP-
GHP with 60 µl aceton
G1
20
16.7
3
20.8
1
5
epN1
onditio
1 Amide
itrile
5 min -
80 min -
83 min 1
85 min -
115 min
30 in 300
itrile and
G
30 40
25.2
1
29.6
7
33.9
7
38.0
7
HPLC Profile of
ns
(Cat. no. LS
Solvent B: 2
35-65 % B
65-100 % B
00 % B
100-35 % B
35 % B
Excitation wavelengt
µl analytical grade w
20 µl water.
G10
50 60 70 80 90 100 110 116
min
41.8
5
45.3
2
48.4
9
51.3
9
54.1
2
56.5
858
.82
60.9
362
.88
64.6
766
.38
67.9
169
.39
70.7
371
.95
73.1
474
.22
2AB Labeled Glucose Homopolymer (Cat. # : CAB-GHP-30, Lot
-N1-4.6x250) Flow: 0.4 ml/min Temperature: 40 °C
50 mM ammonium formate pH 4.4
h: 360 nm Emission wavelength: 425 nm
ater. Approximately 10 µl of reconstituted sample is used per hplc run. Inject 10 µl
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