Determination of quantity of protein in given solution Colorimetric protein assay Absorbance w path length * Concentration Absorbance w Concentration (Path length: Constant)…

1. SpectrophotometryReading:Ninfa & Ballou, Ch. 3Quantification of Protein ConcentrationReading: Ninfa & Ballou, Ch. 4 2. Objectives• The electromagnetic spectrum•…

Slide 1 Slide 2 1 Computer-aided Vaccine and Drug Discovery G.P.S. Raghava Understanding immune system Breaking complex problem Adaptive immunity Innate Immunity Vaccine…

Slide 1Monomerization of Homing Endonucleases Targets: CreI, MsoI, and CeuI. Goal: Generation of catalytically active monomeric HEs by connecting two copies of protein with…

Slide 1CZ5225 Methods in Computational Biology Lecture 6-8: Protein Structure Prediction Methods. Chen Yu Zong Tel: 6874-6877 Email: [email protected] http://xin.cz3.nus.edu.sg…

1.UniProt and the Semantic WebChimezie Ogbuji 2. ‘Omics’ Data Challenges  Advances in protein science is a major catalyst in the exploding availability of bioinformatics…

1.The second large class of proteins distinct from globular proteins are the membrane proteins. It is estimated that 20-30% of all genes in most genomes encode for membrane…

1. Computer Programs for Biological Problems: Is itService or Science ? G. P. S. Raghava  Simple Computer Programs Immunological methods Methods in molecular biology…

1. 1 1 Introduction The important thing in science is not so much to obtain new facts as to discover new ways of thinking about them. [W. L. Bragg]1.1 Classical Approaches…

Overview of Protein Purification and Characterization AIMS AND OBJECTIVES Protein purification has an over 200-year history: the first attempts at isolating substances from…