YOU ARE DOWNLOADING DOCUMENT

Please tick the box to continue:

Transcript
Page 1: b.tech Biotech III Yr i Sem_0

BIOTECHNOLOGY SYLLABUS

SCHEME OF INSTRUCTION AND EXAMINATION

B.Tech. (Biotech) – III YEAR -I Semester

SUBJECT CODE

SUBJECTS

PERIODS/ WEEK

MARKSDURATION OF EXAM

The / Lab Sessional Exams. Hrs.

BTT.3.101Protein Engineering and Enzyme Technology

4 30 70 3

BTT.3.102Bio-fluid Mechanics and Heat Transfer

4 30 70 3

BTT.3.103Probability and Statistics

4 30 70 3

BTT.3.104Plant and Animal Biotechnology

4 30 70 3

BTT.3.105Bio-reaction Engineering

4 30 70 3

BTT.3.106Fermentation Technology

4 30 70 3

BTP.3.107Bio-reaction Engineering Lab

4 25 50 4

BTP.3.108Fluid Mechanics and Heat Transfer Lab

4 25 50 4

BTP.3.109 Fermentation Lab 4 25 50 4

255 570

Page 2: b.tech Biotech III Yr i Sem_0

ROTEIN ENGINEERING & ENZYME TECHNOLOGY

Subject code : BTT.3.101 Sessional : 30

Periods / Week : 4 Examination : 70

Nature of Examination : Theory Exam Duration : 3 Hrs

UNIT- I PROTEIN STRUCTURE AND FUNCTIONS

Peptide bond- Structure, functions, physical and chemical properties, chemical synthesis of peptides; liquid phase and solid phase techniques; Proteins-classification & Biological functions; Physico-chemical properties, forces stabilizing protein structure-primary structure and its determination, helical-p-pleated structure; Ramachandran plot; super secondary structure, tertiary & quaternary structure; myoglobin and hemoglobin, Lysozyme, Ribonuclease A, Carboxy Peptidase, Chymotrypsin, Sickle cell hemoglobin; structure and functional relationship; Fibrous protein (Collagen).

UNIT-II PROTEIN BIOSYNTHESIS AND STRUCTURE PREDICTION

Methods of protein isolation, purification and Quantification; large scale synthesis of proteins, design and synthesis of peptides, use of peptides in biology, methods of detection and analysis of proteins; examples of engineered proteins, protein design, and examples. Post translation modification, protein transport, Inhibitors of Protein Synthesis. Random, site directed catalytic affectivity; Structure prediction and modeling of proteins:

UNIT- III PRODUCTION AND APPLICATIONS OF ENZYMES

Production and purification of crude Enzyme extracts from plant, animal and microbial sources; Methods of characterization of enzymes; development of enzymatic assays. Production of recombinant proteins (Insulin, Interleukin, Interferon) having therapeutic and diagnostic applications; Important commercial enzymes; Proteases; Amylases; Lipases; Cellulases; Pectinases; Isomerases and its applications in food, pharmaceutical and other industries; Enzymes for analytical and diagnostic purposes; Design of enzyme electrodes and their application as biosensors in industry health care and environment.

Page 3: b.tech Biotech III Yr i Sem_0

UNIT- IV MECHANISMS AND KINETICS OF ENZYME ACTION

Mechanisms of enzyme action; Concept of active site and energetics of enzyme substrate complex formation; Specificity of enzyme action; Kinetics of single substrate reactions; Turn over number; Estimation of Michaeli-Menten parameters; Multi substrate reaction mechanisms and kinetics; Types of inhibition - Allosteric regulation of enzymes; Deactivation of kinetics.

UNIT- V ENZYME IMMOBILIZATION & MASS TRANSFER EFFECTS IN IMMOBILISED ENZYME SYSTEMS

Physical and chemical techniques for enzyme immobilization - adsorption, matrix entrapment, encapsulation, cross-linking, covalent binding etc., examples; Advantages and disadvantages of different immobilization techniques; Overview of applications of immobilized enzyme systems; Applications of enzymes in analysis; Analysis of Film and pore Diffusion Effects on kinetics of Immobilized Enzyme Reactions; Formulation of dimensionless groups and calculation of Effectiveness Factors.

EXAMINATION: One question from each unit with internal choice

TEXT BOOKS:

1. Principles of Biochemistry- Lenhinger, David Nelson, W.H.Freeman, (2006).2. Enzyme Technology- Palmer Trevor, E.W.P, (2004).3. Fundamentals of Biochemistry by J.L. Jain, Chand (S.) & Co Ltd , India ((1999)).

REFERENCE BOOKS:

1. Biochemistry- Voet and Voet J.G, 2nd edition, John C.Wiley & Sons (1994).2. Biochemical Engineering James M. Lee eBook Version 2.2. ii © (2001) by James M.

Lee, Gerald Reed , Steve Taylor Third Edition, Academic Press.3. Enzymes by Paul R. Mathewson Eagan Press Handbook Series (1998). 4. Biocatalysis - Fundamentals and Applications Edited by Bommarius, Andreas Sebastian;

Riebel, Bettina R. Wiley-VCH (2004).5. Biocatalysts and Enzyme Technology by Klaus Buchholz, Volker Kasche, Uwe Theo

Bornscheuer, Wiley (2005).6. Enzyme Technology by Ashok Pandey, Collins and others, Springer, (2006).

Page 4: b.tech Biotech III Yr i Sem_0

Bio-fluid Mechanics and Heat Transfer

Subject code : BTT.3.102 Sessional : 30

Periods / Week : 4 Examination : 70

Nature of Examination : Theory Exam Duration : 3 Hrs

UNIT-I

Basic Concepts in Flow of Fluids: Introduction, Nature of fluid, Rheology of fluids -Newton's law of viscosity. Concept of Newtonian and non-Newtonian fluids- Different types of non-Newtonian fluids with examples in bioprocessing. Measurement of viscosity using extrusion rheometer, plate and cone viscometer, coaxial cylinder viscometer etc.

UNIT-II

Flow field, Friction losses in laminar flow through a circular tube (Hagen-Poiseuille equation), Friction losses in turbulent flow (Fanning equation), Pumping of fluids Flow through pipes, average velocity, flow regimes, boundary layer concept. Laminar and turbulent flow -characterization by Reynold's number, pressure drop due to skin friction and form friction, friction factor chart, Hageri -Poiseuille equation.

UNIX-III

Flow past immersed bodies: Definition of drag and drag coefficient. Friction in flow through beds of solids, Brief introduction to flow of compressible fluids. Flow measuring and monitoring systems- valves, bends, elbows, prevention of leaks, mechanical seals, stuffing box. Flow measuring devices-manometers, orifice meter, venture meter and rotameter. Brief description of Pumps and Blowers

UNIT-IV

Basic Concepts in "Heat Transfer: Introduction and Mechanisms of heat transfer; Conduction heat transfer (through slab, cylinder & Sphere); Conduction through solids in series, Forced convection heat transfer inside pipes, Introduction to radiation heat transfer, Chilling and freezing of food and Biological materials. Heat .transfer correlations, and calculations, basic heat exchange equipment

Page 5: b.tech Biotech III Yr i Sem_0

UNIT-V

Basic Concepts in Evaporation and Condensation: Introduction, Types of evaporation equipment and operation methods; Overall heat transfer coefficients in evaporators; simple material balances. Calculation methods for single effect evaporators, Evaporation of biological materials. Types of condensation, numerical problems and condensation equipment.

EXAMINATION: One question from each unit with internal choice

TEXT BOOKS:

1. Unit operations in Chemical Engineering, W L McCabe & J C Smith, 6th Ed., McGraw Hill Intl. Ed.(2005).

2 Transport Processes and Unit Operations by Christie J. Geankoplis, 3rd Ed., Prentice Hall India Pvt. Ltd.

3. “Basic Fluid Mechanics”, Kothandaraman C P and Rudramoorthy. R., New Age International Publishers, New Delhi, (1998).

4. “Fundamentals of Engineering Heat and Mass Transfer” by Sachdeva. R.C., New Age International Publishers. New Delhi, (1996)

REFERENCE BOOKS:

1. Introduction to Chemical Engineering by S. K. Ghosal, S. K. Sanyal and S. Dutta, TMH publications, (1993).

2. Computational Fluid Mechanics and Heat Transfer, by Richard Pletcher Second Edition, Pub: Taylor & Francis; (1997)

3. "Mechanics of Fluids". By Irving H Shames. 3rd Ed., McGraw-Hill Publishing Co.. New York, (1992).

4. “Rate Processes in Metallurgy”, Mohanty A.K., Prentice Hall of India, New Delhi, (2000)

5. “Transport Phenomena “by Byron Bird R., Stewart.W.E., John-Wiley & Sons Inc., (1994).

6. Engineering Thermofluids: Thermodynamics, Fluid Mechanics, and Heat Transfer By Mahmoud Massoud Publisher: Springer (2005).

7. Introduction To Thermal Systems Engineering: Thermodynamics, Fluid Mechanics And Heat Transfer, by Michael J. Moran, Howard N. Shapiro, Bruce R. Munson, David P. DeWitt, John Wiley & Sons edition (2003).

Page 6: b.tech Biotech III Yr i Sem_0

8. Fundamentals of Fluid Mechanics by Bruce R. Munson, Theodore H. Okiishi, Donald F. Young, John Wiley & Sons Inc, (2005).

9. Fluid Mechanics with Engineering Applications by E. John Finnemore, Joseph B. Franzini, McGraw-Hill College (2001).

10. Introduction to Thermal Systems Engineering:Thermodynamics, Fluid Mechanics, and Heat Transfer by Michael J. Moran, Bruce R. Munson, Howard N. Shapiro, David P. Dewitt , John Wiley & Sons Inc, (2002).

Page 7: b.tech Biotech III Yr i Sem_0

PROBABILITY AND STATISTICS

Subject code : BTT.3.103 Sessional : 30

Periods / Week : 4 Examination : 70

Nature of Examination : Theory Exam Duration : 3 Hrs

UNIT-1 PROBABILITY AND PROBABILITY DISTRIBUTIONS

Probability: Sample space and events - counting - probability - The Awicens of probability - some elementary theorems - conditional probability - mathematical; Probability Distributions: Random variables - The Binomial Distribution- The mean and the Variance of a probability Distribution - Chebyshev's theorem - The Poisson approves - motion of the binomial distribution - Poisson procession.

UNIT -II PROBABILITY DENSITIES AND SAMPLING DISTRIBUTIONS

Probability Densities: Continuous Random Variables - The normal Distribution - The normal Approximation to the Binomial Distribution. Sampling Distributions: Populations and Samples; The sampling Distribution of the Mean (known) - The sampling distribution of the Mean (unknown) - The sampling Distribution of the Variance.

UNIT -III INTERFERENCE

Interference Concerning Means: Point Estimation - Interval Estimation - Bayesians Estimation - Tests of Hypothesis and Significant Tests - Hypothesis concerning one mean - Inferences concerning two means; Interference concerning proportions: Estimation of proportions; Hypothesis concerning one proportion.

UNIT IV CURVE FITTING

Curve fitting; The method of least squares - Inferences based on the least squares estimators -curve linear regression- Multiple regression -correlation -Multiple linear regression.

Page 8: b.tech Biotech III Yr i Sem_0

UNIT V BIOSTATISTICS

Statistical basis of biological assays: Response-Dose Metameter, Delusion Assays, direct and Indirect Assays, Standard line Interpolation assay, Parallel line Assay (4 Point and 6 Point Assays) and Slope Ratio Assay; Statistical Quality Control Charts and Application of Statistical Concepts in Biological Sciences.

EXAMINATION: One question from each unit with internal choice

TEXT BOOKS:

1. Miller & Freud's probability and statistics for Engineers, 5th ed., Richard A. Johnson, PHI.

2. Probability and statistics for engineers. R. Miller, J. E.Freund, and R. Johnson. Prentice Hall, (1990).

3. Biostatistics: A Foundation for Analysis in the Health Sciences (Wiley Series in Probability

and Statistics), by Wayne W. Daniel, Wiley; 7TH edition (1999).

REFERENCE BOOKS:

1. Probability and Statistics, M.R. Spiegel, Schaum Series, Tata McGraw-Hill, (2007) 9th RP.

2 Statistics for Biologists, by Campbell, R.C., Cambridge University Press

3.Introductory Probability and Statistical Applications- K. Paul Meyer.

4. Biometry: The Principles and Practice of Statistics in Biological Research

by Robert R. Sokal , F. James W.H. Freeman & Company; 2nd edition(1994).

5. Biostatistical Analysis (5th Edition) by Jerrold H. Zar Publisher: Prentice Hall;

(2009).

6. Essentials of Statistics (3rd Edition) Addison Wesley (2006)

7. Experimental Design and Data Analysis for Biologists by Gerry P. Quinn, Michael J. Keough. Cambridge University Press; 1st Edition (2002)

8. Biostatistics: A Methodology for the Health Sciences (Wiley Series in Probability & Mathematical Statistics: Applied Probability & Statistics) Wiley-Interscience (1996)

9. Accelerated Testing: Statistical Models, Test Plans, and Data Analysis (Wiley Series in Probability and Statistics Wiley-Interscience February (1990)

10. Statistical Methods for Reliability Data (Wiley Series in Probability and Statistics)

Wiley-Interscience; 1st Edition (1998).

Page 9: b.tech Biotech III Yr i Sem_0

PLANT AND ANIMAL BIOTECHNOLOGY

Subject code : BTT.3.104 Sessional : 30

Periods / Week : 4 Examination : 70

Nature of Examination : Theory Exam Duration : 3 Hrs

UNIT I TISSUE CULTURE

Introduction to cell and tissue culture; Tissue culture media (composition, preparation); Initiation and maintenance of callus and cell suspension culture, organogenesis; Protoplast isolation, culture and fusion.

UNIT II TISSUE CULTURE IN CROP IMPROVEMENT

Micropropagation for virus-free plants, Somaclonal variation, Somatic Hybridization, Haploids in plant breeding, Germplasm conservation (Cryopreservation).

UNIT III IN VITRO PRODUCTION OF SECONDARY METABOLITES.

Production of secondary metabolites from plant cell cultures; Processes for enhancing the production of secondary metabolites. Technology of plant cell culture for production of chemicals; Bioreactors systems and models for mass cultivation of plant cells

UNIT-IV ANIMAL CELL TISSUE CULTURE

History and scope of animal cell tissue culture. advantages and disadvantages of tissue culture; laboratory facilities for animal tissue culture; the substrate on which cells grow; treatment of substrate surfaces. Feeder layers on substrates. The gas phase for tissue culture. Culture media for cells and tissues. Cell culture procedures. Tissue culture Slide, Flask and test tube cultures, Organ culture, Whole embryo culture.

Page 10: b.tech Biotech III Yr i Sem_0

UNIT-V CELL LINES, CLONING AND GENE TRANSFER

Disaggregating (Enzymatic and Mechanical) of tissue and Primary culture. Culture cells and evolution of cell lines. Maintenance of cultures- Cell lines. Cloning of cell lines. Large scale cultures in Biotechnology. Somatic cell fusion. Gene transfer or Transfection (using Eggs and cultured stem cells); targeted gene transfer; Transgenic animals (Mice, Sheep, Pig, Rabbit, Goat, Cow and fish). Application of animal cell culture; Bioreactors for animal cell culture; Mammalian cell products; viral vaccines produced from animal cell cultures.

EXAMINATION: One question from each unit with internal choice

TEXT BOOKS:

1. Plant Tissue Culture: Theory and Practice: Bhojwani and Razdan (2004).2. Culture of Animal Cells: A Manual of Basic Technique by R. Ian Freshney Wiley Liss ,

Wiley, John & Sons, (2005).3. Animal Cell Culture Practical approach Ed. John, RW Masters. Oxford Publications. 4. Plant propagation by Tissue culture Part I, II and III in practice. EJ George Exegetics Ltd

England5. Biotechnology by U.Satyanarayana,(2008), Books and Allied(p)Ltd.6. Plant Tissue Culture Book, Víctor M. Loyola-Vargas, C. De-la-Peña, R. M. Galaz-Ávalos

and F. R. Quiroz-Figueroa, Humana Press Springer, (2008).

REFERENCE BOOKS:

1. Principles of Plant Biotechnology An introduction to Genetic Engineering in Plants- Mantal S.H. Mathews J.A., Mickee R.A., Blackwell Scientific Pubs, 1989.

2. Revolution in Biotechnology- Cambridge University Press, 1989.3. Plant Genetic Engineering by John H. Dodds Cambridge University Press, (1985).4. Culture of Animal Cells: A Manual of Basic Techniqueby R. Ian Freshney , Wiley-Liss; 5

edition (2005). 5. Biotechnology in Plant Improvement P.C. Trivedi (ed.) Pub: Pointer Publishers, (2007)6. Plant Biotechnology by Michael W. Fowler , Graham S. Warren Pergamon; 1st Edition

(1992)7. Plant biotechnology and transgenic plants By Kirsi-Marja Oksman-Caldentey, Wolfgang

Barz, (2006)8. Introduction to Plant Biotechnology by H. S. Chawla, Science Publishers; 2nd Edition

(2002).

Page 11: b.tech Biotech III Yr i Sem_0

9. Animal Biotechnology: Comprehensive Biotechnology, by Lorne A Babiuk; John P Phillips; Murray Moo-Young Publisher: Oxford, New York: Pergamon Press, 1989.

10. Plant Biotechnology A Practical Approach H. S. Chawla, Science Pub. (2003).11. Cell Separation Fundamentals, Analytical And Preparative Methods Series: Advances In

Biochemical Engineering / Biotechnology , Vol. 106 Kumar, Ashok; Galaev, Igor Yu; Mattiasson, Bo (Eds.) (2007).

Page 12: b.tech Biotech III Yr i Sem_0

BIOREACTION ENGINEERING

Subject code : BTT.3.105 Sessional : 30

Periods / Week : 4 Examination : 70

Nature of Examination : Theory Exam Duration : 3 Hrs

UNIT-1 INTRODUCTION TO REACTION KINETICS

Concepts of Reaction Kinetics, Types of reaction, order of reaction, The effect of temperature and pH on reaction rate. Rate equations and Reaction mechanisms; Interpretation of batch reactor data, constant volume batch reactor, integral method of analysis of data for reversible and irreversible reactions.

UNIT- II REACTION MECHANISM AND GROWTH KINETICS

Searching for mechanism - Arrhenius equation - Batch reactor analysis for kinetics, (synchronous growth and its application in product production).

Growth Kinetics: Batch growth quantifying cell concentration, growth profiles and kinetics in batch culture, fed batch growth, continuous growth and their growth kinetic quantification, chemostat growth, semi-continuous / exponential feeding strategy.

UNIT- III BIOREACTOR SYSTEMS

Definitions, Differences and similarities between chemical and bioreactors; Classification of bioreactors; Reactor configurations; Description of a conventional bioreactor with all aspects; Design and construction criteria of a bioreactor; Residence time distributions, concentration, and temperature distributions; Models of non-ideal reactors.

UNIT- IV DESIGNING OF BIOREACTORS

Design equations for enzyme reactors, batch growth of microorganisms, Design equation of a plug flow reactor; Design of CSTR with washout concept; Stirred tank reactors with recycle of biomass; Continuous stirred tank fermentors in series without and with recycle of biomass; Estimation of kinetic parameters,

UNIT- V MULTIPHASE BIOREACTORS

Page 13: b.tech Biotech III Yr i Sem_0

Different types of reactors: Cell lift reactor, Multi purpose tower reactor, Liquid impelled loop reactor, Pumped tower loop reactor, Fluidized-bed reactor, Packed bed reactor, Bubble-column reactors, Airlift reactors.

Animal and plant cell reactor technology- Environmental requirements for animal cell cultivation, reactors for large-scale production using animal cells, plant cell cultivation.

EXAMINATION: One question from each unit with internal choice

TEXT BOOKS:

1. Biochemical Engineering by Harvey W. Blanch, Douglas S. Clark CRC; 1st Edition (1997).

2. Biochemical Engineering Fundamentals: Solutions Manual by James E. Bailey, David F. Ollis. McGraw-Hill Education (1979).

REFERENCE BOOKS:

1. Advances in Biochemical Engineering / Biotechnology Series Ed.: Scheper, T.,

2. Biochemical Engineering and Biotechnology Handbook, by Bernard Atkinson Ferda Mavituna, Grove's Dictionaries; 2nd Edition (1992) .

3. Bioreactor Systems for Tissue Engineering Series: Advances in Biochemical Engineering / Biotechnology , Vol. 112 Kasper, Cornelia; van Griensven, Martijn; Pörtner, Ralf , (2009), Springer.

4. Biochemical Engineering by Harvey W. Blanch, Douglas S. Clark CRC; 1st Edition (1997).

5. S.Aiba, A.E. Humphrey and N.R. MHH, "Bio-chemical Engineering", Second Edn. Academic Press, 1973.

FERMENTATION TECHNOLOGY

Page 14: b.tech Biotech III Yr i Sem_0

Subject code : BTT.3.106 Sessional : 30

Periods / Week : 4 Examination : 70

Nature of Examination : Theory Exam Duration : 3 Hrs

UNIT-I INTRODUCTION TO FERMENTATION PROCESSES

The range of fermentation processes; the chronological development of fermentation industry; Industrial applications; Future trends in fermentations; Aseptic transfer of spore suspension; Transfer of inoculums from seed tank to Fermentor.

UNIT- II FERMENTATION PROCESSES

General requirements of fermentation processes, Basic design and construction of fermentor and ancillaries, Main parameters to be monitored and controlled in fermentation processes; An overview of aerobic and anaerobic fermentation processes and their application in the biotechnology industry, solid-substrate, slurry fermentation and its applications,

UNIT- III AERATION AND AGITATION IN FERMENTATIONS

Basic Mass transfer concepts; Oxygen transfer from gas bubble to cells; Oxygen transfer in fermentations; Bubble aeration and Mechanical agitation; Correlations for mass transfer coefficients; Gas Hold up; Power consumption concepts; Determination of oxygen transfer rates, KLa values; Other Factors affecting the values of mass transfer coefficients in fermentation vessels;

UNIT- IV SCALE UP AND RHEOLOGY IN FERMENTATIONS

Scale up of fermentation processes; Principles, theoretical considerations and techniques used; Scale down methods; The Rheology of fermentation broths; Rheological models; Measurement of Theological parameters; Rheological Control of fermentations. Mixing concepts, power requirement for mixing and improvement of mixing in fermentations.

UNIT - V FERMENTORS

Page 15: b.tech Biotech III Yr i Sem_0

Batch, Fed-batch and Continuous Fermentation systems; Dual and multiple fermentations; Comparison between batch and continuous fermentations; Steady state, unsteady state continuous fermentation theories; Examples of continuous fermentation; Practical problems with continuous operations. Monitoring and Control of fermentations, behavior of microbes in different reactors (air lift, fluidized, batch, and continuous fed batch condition).

EXAMINATION: One question from each unit with internal choice

TEXT BOOKS:

1. Principles of Fermentation Technology by Peter F. Stanbury , Stephen J. Hall , Allan Whitaker Butterworth-Heinemann; 2nd Edition (1999)

2. Fundamentals of Biochemical Engineering-Bailey & Ollis Mc. Gr. Hill, 1986

REFERENCE BOOKS:

1. Bioprocess Engineering” by M. Shuler and F. Kargi ((2002)), Prentice Hall of India Pvt. Ltd., New Delhi.

2. Biochemical Engineering by F C Webb Van Nost. Reinhold (1963)

3. Biochemical Engineering by Harvey W. Blanch), Douglas S. Clark CRC; 1st Edition (1997).

4. Fermentation Technology (2008) by M.l. Srivastava, Narosa Publishing House, (2008).

5. Practical Fermentation Technology by Brian McNeil , Linda Harvey, Wiley

(2008).

6. “Biotechnology: A Text Book of Industrial Microbiology” 2nd Edition, by W. Crueger & A. Crueger (2000) Panima Publishing Corporation, New Delhi/Bangalore.

7. Handbook of Food and Beverage Fermentation Technology by Hui , Y.H. Hui , Lisbeth Meunier-Goddik, Ase Slovejg Hansen , Jytte Josephsen , Wai-Kit Nip , Peggy S. Stanfield , Fidel Toldra , CRC Press LLC; 1 edition (2004).

8. Essentials of Biochemistry. U.Sathyanarayana. Books and Allied (P) Ltd., Kolkata (1st Edition), (2002).

Page 16: b.tech Biotech III Yr i Sem_0

BIOPROCESS ENGINEERING LAB- I

Subject code : BTP.3.107 Sessional : 25

Periods / Week : 4 Examination : 50

Nature of Examination : Theory Exam Duration : 4 Hrs

LIST OF EXPERIMENTS

1. Preparation of Buffers.

2. Isolation and Extraction of Enzymes

3. Effect of pH on enzyme activity.

4. Effect of Temperature on enzyme activity.

5. Effect of substrate concentration on enzyme activity.

6. Effect of time interval on enzyme activity.

7. Evaluation of enzyme kinetic parameters (Michaeli-Menten approach)

8. Kinetic studies of enzyme inhibition.

9. Deactivation kinetics

10. Enzyme/cell immobilization - matrix entrapment, ionic and cross linking; column

packing;

11. Determination of growth curve of a supplied microorganism and to determine substrate

degradation profile.

12. Development of enzyme assays.

13. Analysis of mass transfer effects on kinetics of immobilized enzyme reactions;

14. Bioconversion studies with immobilized - enzyme packed - bed reactors.

Page 17: b.tech Biotech III Yr i Sem_0

FLUID MECHANICS AND HEAT TRANSFER LAB

Subject code : BTP.3.108 Sessional : 25

Periods / Week : 4 Examination : 50

Nature of Examination : Theory Exam Duration : 4 Hrs

LIST OF EXPERIMENTS

1 Determination of discharge coefficient for orifice meter and venturi meter and their

variation with Reynolds number

2 Determination of weir meter constant K for v-notch and rectangular notch

3 Calibration of rotameter and study of variation of flow rate with tube to float diameter

4 Determination of viscosity of Glycerol - water solutions at different temperatures

5 Determination of friction factor for flow of water through annulus using Farmings and

Davos equations.

6 Determination of friction factor for flow through straight pipes of different diameters and

study of variation of friction factor with Reynolds number.

7 Determination of friction losses in pipe fittings

8 Determination of Thermal conductivity of homogeneous wall insulating powder under

steady state conditions.

9 Determination of interface temperatures in composite wall under steady state conditions.

10 Determination of heat transfer coefficient in Natural convection.

11 Determination of over all heat transfer coefficient in unsteady state heat transfer

12 Determination of inside heat transfer coefficient in coil heat exchangers

13 Determination of over all heat transfer coefficient and effectiveness in a Double pipe heat

exchange

14 Determination of heat transfer area in a 1-2- shell and tube heat exchanges

15 Determination of heat transfer coefficient on a single tube by film wise and drop wise

condensation.

Page 18: b.tech Biotech III Yr i Sem_0

FERMENTATION TECHNOLOGY LAB

Subject code : BTP.3.109 Sessional : 25

Periods / Week : 4 Examination : 50

Nature of Examination : Theory Exam Duration : 4 Hrs

LIST OF EXPERIMENTS

1. Culturing of different types of microorganisms (bacteria, yeast, fungi) used in the

production of commercially important products

2. Preparation of Media

3. Sterilization of Media and Air

4. Formulation of simple and complex culture media for fermentations.

5. Growth of E. coli DH5ά in batch culture.

6. Fed batch culture techniques

7. Estimation of growth kinetics.

8. Study of Growth substrate utilization

9. Product formation kinetics in shake flask cultures.

10. Bioreactor instrumentation and control.

11. Microbial production of fine chemicals.

12. Estimation of biomass (dry weight); substrate and product analysis;


Related Documents