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Week 12w This Week: Unknown Project Exp 45: Chemical Agents of Control: Disinfectants and Antiseptics Part B: Agar Plate- Sensitivity Method Last Week: Exp 43: Chemical Agents of Control: Chemotherapeutic Agents; Part A: Kirby Bauer Antibiotic Sensitivity Test
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Week 12w This Week: Unknown Project Exp 45: Chemical Agents of Control: Disinfectants and Antiseptics Part B: Agar Plate-Sensitivity Method Last Week:

Mar 26, 2015

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Page 1: Week 12w This Week: Unknown Project Exp 45: Chemical Agents of Control: Disinfectants and Antiseptics Part B: Agar Plate-Sensitivity Method Last Week:

Week 12wWeek 12w

This Week:

Unknown Project

Exp 45: Chemical Agents of Control: Disinfectants and Antiseptics Part B: Agar Plate-Sensitivity Method

Last Week:

Exp 43: Chemical Agents of Control:

Chemotherapeutic Agents;

Part A: Kirby Bauer Antibiotic

Sensitivity Test

Page 2: Week 12w This Week: Unknown Project Exp 45: Chemical Agents of Control: Disinfectants and Antiseptics Part B: Agar Plate-Sensitivity Method Last Week:

Exp 43: Chemical Agents of Control: Chemotherapeutic Agents

• Some chemotherapeutic agents have a limited spectrum of activity being effective against only one group of microorganisms.

• Other chemotherapeutic agents exhibit broad spectrum activity against a range of microorganisms.

• Drug susceptibilities on many pathogenic microorganisms are known, but it is sometimes necessary to test several agents to determine the drug of choice.

Page 3: Week 12w This Week: Unknown Project Exp 45: Chemical Agents of Control: Disinfectants and Antiseptics Part B: Agar Plate-Sensitivity Method Last Week:

Exp. 69: Species Identification of Unknown Bacterial Cultures

• Unknown project overview.

• Requisition Forms.

• Written report.

• Part 2: – Gram Stain– Differential Media – Biochemical Tests

Page 4: Week 12w This Week: Unknown Project Exp 45: Chemical Agents of Control: Disinfectants and Antiseptics Part B: Agar Plate-Sensitivity Method Last Week:

Exp 45: Chemical Agents of Control: Disinfectants and Antiseptics

• Disinfectants and antiseptics- chemicals used to prevent contamination and infection

• Efficacy is influenced by:– Concentration– Length of exposure– Type of microbial population to be destroyed– Environmental conditions

Page 5: Week 12w This Week: Unknown Project Exp 45: Chemical Agents of Control: Disinfectants and Antiseptics Part B: Agar Plate-Sensitivity Method Last Week:

Exp 45: Chemical Agents of Control: Disinfectants and Antiseptics Part B: Agar Plate-Sensitivity Method

Media: 2 TSA plates, 2 swabsCulture: EC & SAProcedure: (refer to pg. 307)• Label plate w/org. Label plate with test solution.• Heavily inoculate entire surface of plate.• Saturate disks with test solution.• Use forceps to fix disks to agar.

- mouth wash- peroxide

- iodine- hand soap (lab)

• Incubate plates at 37oC for 24hrs in inverted position.

Week 12WWeek 12W

PER TABLE

Page 6: Week 12w This Week: Unknown Project Exp 45: Chemical Agents of Control: Disinfectants and Antiseptics Part B: Agar Plate-Sensitivity Method Last Week:
Page 7: Week 12w This Week: Unknown Project Exp 45: Chemical Agents of Control: Disinfectants and Antiseptics Part B: Agar Plate-Sensitivity Method Last Week:

Epidemiology: the science of when and where diseases occur

and how they are transmitted

•Descriptive (data on occurrence)

•Analytical (probable cause)

•Experimental (hypothesis, test)

CDC, Center for Disease Control, US Public Health Service