Week 12w This Week: Unknown Project Exp 45: Chemical Agents of Control: Disinfectants and Antiseptics Part B: Agar Plate- Sensitivity Method Last Week: Exp 43: Chemical Agents of Control: Chemotherapeutic Agents; Part A: Kirby Bauer Antibiotic Sensitivity Test
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Week 12w This Week: Unknown Project Exp 45: Chemical Agents of Control: Disinfectants and Antiseptics Part B: Agar Plate-Sensitivity Method Last Week:
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Week 12wWeek 12w
This Week:
Unknown Project
Exp 45: Chemical Agents of Control: Disinfectants and Antiseptics Part B: Agar Plate-Sensitivity Method
Last Week:
Exp 43: Chemical Agents of Control:
Chemotherapeutic Agents;
Part A: Kirby Bauer Antibiotic
Sensitivity Test
Exp 43: Chemical Agents of Control: Chemotherapeutic Agents
• Some chemotherapeutic agents have a limited spectrum of activity being effective against only one group of microorganisms.
• Other chemotherapeutic agents exhibit broad spectrum activity against a range of microorganisms.
• Drug susceptibilities on many pathogenic microorganisms are known, but it is sometimes necessary to test several agents to determine the drug of choice.
Exp. 69: Species Identification of Unknown Bacterial Cultures
• Unknown project overview.
• Requisition Forms.
• Written report.
• Part 2: – Gram Stain– Differential Media – Biochemical Tests
Exp 45: Chemical Agents of Control: Disinfectants and Antiseptics
• Disinfectants and antiseptics- chemicals used to prevent contamination and infection
• Efficacy is influenced by:– Concentration– Length of exposure– Type of microbial population to be destroyed– Environmental conditions
Exp 45: Chemical Agents of Control: Disinfectants and Antiseptics Part B: Agar Plate-Sensitivity Method
Media: 2 TSA plates, 2 swabsCulture: EC & SAProcedure: (refer to pg. 307)• Label plate w/org. Label plate with test solution.• Heavily inoculate entire surface of plate.• Saturate disks with test solution.• Use forceps to fix disks to agar.
- mouth wash- peroxide
- iodine- hand soap (lab)
• Incubate plates at 37oC for 24hrs in inverted position.
Week 12WWeek 12W
PER TABLE
Epidemiology: the science of when and where diseases occur
and how they are transmitted
•Descriptive (data on occurrence)
•Analytical (probable cause)
•Experimental (hypothesis, test)
CDC, Center for Disease Control, US Public Health Service