Top Banner
Overview In this application note, we developed an LC-MS/MS method to analyze chloroquine (CQ), and its metabolite desethylchloroquine (DCQ), and hydroxychloroquine (HCQ), resulting in acceptable resolution and separation of the analytes. Analysis of Chloroquine and Its Metabolite Desethylchloroquine by LC-MS/MS using Kinetex® Core-Shell F5 LC Column Dawn Chen Phenomenex, Inc., 411 Madrid Ave., Torrance, CA 90501 USA Page 1 of 2 Have questions or want more details on implementing this method? We would love to help! Visit www.phenomenex.com/ChatNow to get in touch with one of our Technical Specialists LC-MS/MS Conditions Column: Dimension: Part No.: Mobile Phase: Gradient: Flow Rate: Temperature: Detection: Sample: Kinetex 2.6 µm F5 50 x 2.1 mm 00B-4723-AN A: Water with 0.1 % Formic Acid B: Methanol % B Time (min) 2 0.0 7 2.0 15 3.0 40 3.2 95 4.2 2 4.3 2 6.0 0.5 mL/min 20 °C MS/MS (MRM, SCIEX API 4000 QTRAP®) Chloroquine mix 1. Desethylchloroquine (DCQ) 2. Hydroxchloroquine (HCQ) 3. Chloroquine (CQ) MS/MS Parameters Ion Source Turbo Spray, Positive CUR 35 CAD Medium EP 10 GS1 60 GS2 60 IS 550 Source Temp 650 °C iHe ON CEM 2200 (Detector, negative) 1 MS/MS Transitions CXP CE DP Dwell Q3 Q1 ID CQ (Quan) 10 33 65 50 247.1 320 CQ (Qual) 10 33 65 50 142.1 320 HCQ (Quan) 10 33 55 50 247.1 336.3 HCQ (Qual) 10 33 55 50 158.1 336.3 DCQ (Quan) 13 30 51 50 179.2 292.3 DCQ (Qual) 13 30 51 50 247.1 292.3 2 3 App ID 25873
2

v o Ç ] } ( Z o } } µ ] v v / D } o ] Z Ç o Z o } } µ ] v Ç > …...v o Ç ] } ( Z o } } µ ] v v / D } o ] Z Ç o Z o } } µ ] v Ç > rD^ lD^ µ ] v P < ] v Æ } r^

Jun 20, 2020

Download

Documents

dariahiddleston
Welcome message from author
This document is posted to help you gain knowledge. Please leave a comment to let me know what you think about it! Share it to your friends and learn new things together.
Transcript
Page 1: v o Ç ] } ( Z o } } µ ] v v / D } o ] Z Ç o Z o } } µ ] v Ç > …...v o Ç ] } ( Z o } } µ ] v v / D } o ] Z Ç o Z o } } µ ] v Ç > rD^ lD^ µ ] v P < ] v Æ } r^

Overview

In this application note, we developed an LC-MS/MS method to analyze chloroquine (CQ), and its metabolite desethylchloroquine (DCQ), and hydroxychloroquine (HCQ), resulting in acceptable resolution and separation of the analytes.

Analysis of Chloroquine and Its Metabolite Desethylchloroquine by LC-MS/MS using Kinetex® Core-Shell F5 LC ColumnDawn ChenPhenomenex, Inc., 411 Madrid Ave., Torrance, CA 90501 USA

Page 1 of 2Have questions or want more details on implementing this method? We would love to help!Visit www.phenomenex.com/ChatNow to get in touch with one of our Technical Specialists

LC-MS/MS ConditionsColumn:

Dimension:Part No.:

Mobile Phase:

Gradient:

Flow Rate:Temperature:

Detection:Sample:

Kinetex 2.6 µm F550 x 2.1 mm 00B-4723-ANA: Water with 0.1 % Formic AcidB: Methanol

% BTime (min)20.072.0

153.0403.2954.224.326.0

0.5 mL/min20 °CMS/MS (MRM, SCIEX API 4000 QTRAP®)Chloroquine mix 1. Desethylchloroquine (DCQ)2. Hydroxchloroquine (HCQ)3. Chloroquine (CQ)

MS/MS Parameters

Ion Source Turbo Spray, PositiveCUR 35CAD MediumEP 10GS1 60GS2 60IS 550Source Temp 650 °CiHe ONCEM 2200 (Detector, negative)

1

MS/MS Transitions

CXPCEDPDwellQ3Q1ID

CQ (Quan)

10336550247.1320

CQ (Qual)

10336550142.1320

HCQ (Quan)

10335550247.1336.3

HCQ (Qual)

10335550158.1336.3

DCQ (Quan)

13305150179.2292.3

DCQ (Qual)

13305150247.1292.3

2

3

App

ID 2

5873

Page 2: v o Ç ] } ( Z o } } µ ] v v / D } o ] Z Ç o Z o } } µ ] v Ç > …...v o Ç ] } ( Z o } } µ ] v v / D } o ] Z Ç o Z o } } µ ] v Ç > rD^ lD^ µ ] v P < ] v Æ } r^

Page 2 of 2

www.phenomenex.comPhenomenex products are available worldwide. For the distributor in your country/region, contact Phenomenex USA, International Department at [email protected]

Terms and ConditionsSubject to Phenomenex Standard Terms and Conditions, which may be viewed at www.phenomenex.com/TermsAndConditions.TrademarksKinetex is a registered trademark of Phenomenex. SCIEX and QTRAP are registered trademarks of AB SCIEX Pte. Ltd. AB SCIEX™ is being used under license.

FOR RESEARCH USE ONLY. Not for use in clinical diagnostic procedures.© 2020 Phenomenex, Inc. All rights reserved.

TN74

3005

20_W