Susan Hollingsworth Aaron Kaiser Biology 110 Lab # smh092404.

Susan HollingsworthSusan HollingsworthAaron KaiserAaron KaiserBiology 110Biology 110

Lab # smh092404Lab # smh092404

Purpose:Purpose: To observe multiple environments that harbor To observe multiple environments that harbor

bacterial growth and determine which bacterial growth and determine which environment under investigation is most environment under investigation is most conducive to bacterial growthconducive to bacterial growth

Environments under investigation:Environments under investigation:airairsoilsoilwashed/unwashed handswashed/unwashed handsdoor handle of men’s bathroomdoor handle of men’s bathroomraw chickenraw chickenpond waterpond water

Hypothesis:Hypothesis:

Pond Water Pond Water largest and most diverse population of largest and most diverse population of

bacteria due to a vast array of living and bacteria due to a vast array of living and decomposing organisms that are decomposing organisms that are vulnerable to bacterial colonization vulnerable to bacterial colonization

aquatic medium that receives sunlight aquatic medium that receives sunlight (some bacteria are photosynthetic)(some bacteria are photosynthetic)

exposed to air which transports bacterial exposed to air which transports bacterial spores.spores.

Materials:Materials: 7 sterile Petri dishes with nutrient agar7 sterile Petri dishes with nutrient agar Wax pencilWax pencil 9 bacterial inoculating loops9 bacterial inoculating loops Sterile cotton swabSterile cotton swab Piece of raw chickenPiece of raw chicken Soil sampleSoil sample Pond water samplePond water sample Hand soapHand soap Sterile waterSterile water Parafilm stripsParafilm strips Light microscopeLight microscope Dissecting microscopeDissecting microscope incubatorincubator

Method:Method:1.1. Label each Petri dishLabel each Petri dish

2.2. ““Air” sampleAir” sample

3.3. Use Streak Plate method to apply sampleUse Streak Plate method to apply sample

(a) (b) (c)

Figure 1. Isolating bacterial colonies using the “Streak Plate” Figure 1. Isolating bacterial colonies using the “Streak Plate” method.method.

Data Interpretations:Data Interpretations:

4.4. Unwashed/Washed hand sampleUnwashed/Washed hand sample5.5. Recover dish labeled “air.”Recover dish labeled “air.”6.6. Wrap cultures with Parafilm and incubate upside Wrap cultures with Parafilm and incubate upside

down for 1 week.down for 1 week.

The Petri dishes will be analyzed by using colony The Petri dishes will be analyzed by using colony morphology characteristics to see which one has morphology characteristics to see which one has the most species of bacteria. This will be the most species of bacteria. This will be accomplished by identifying and tabulating the accomplished by identifying and tabulating the number of different colonies present in each number of different colonies present in each sample. sample.

ColonyColony SizeSize ShapeShape MarginMargin SurfaceSurface ColorColor

a.a. 6mm6mm IrregularIrregular LobateLobate WrinkledWrinkled Milky whiteMilky white

b.b. 4mm4mm RoundRound WavyWavy SmoothSmooth White center, White center, clear clear surroundingsurrounding

c.c. 13mm13mm IrregularIrregular LobateLobate SmoothSmooth White center, White center, milky white milky white surroundingsurrounding

d.d. 5mm5mm IrregularIrregular WavyWavy SmoothSmooth Yellow, gold, Yellow, gold, clear clear surroundingsurrounding

e.e. 9.5mm9.5mm IrregularIrregular WavyWavy Smooth, Smooth, contoured contoured edgesedges

Tan center, Tan center, white ring, white ring, clear ringclear ring

Table 1. Bacteria colony morphology: Air

Fig. 2 Bacteria present in Air environemnt

ColonyColony SizeSize ShapeShape MarginMargin SurfaceSurface ColorColor

a.a. 1mm1mm RoundRound LobateLobate WrinkledWrinkled Creamy Creamy white to white to slightly slightly yellowyellow

b.b. 5mm5mm RoundRound SmoothSmooth SmoothSmooth whitewhite

ColonyColony SizeSize ShapeShape MarginMargin SurfacSurfacee

ColorColor

a.a. 10mm10mm No No defined defined shapeshape

No No defined defined marginmargin

WrinkledWrinkled ClearClear

b.b. Less than Less than 1mm1mm

IrregularIrregular LobateLobate WrinkledWrinkled Cream Cream whitewhite

Table 2. Bacteria colony morphology: Chicken

Table 3. Bacteria colony morphology: Soil

Fig 3. Bacteria found on Chicken environment

Fig 4. Bacteria found on soil environment

ColonyColony ShapeShape SizeSize MarginMargin SurfaceSurface ColorColor

a.a. 3mm3mm RoundRound LobateLobate SmoothSmooth Creamy Creamy whitewhite

b.b. 4mm4mm RoundRound LobateLobate Smooth Smooth ClearClear

c.c. PunctiformPunctiform RoundRound SmoothSmooth SmoothSmooth whitewhite

Table 5. Bacteria colony morphology: washed hand

Fig 5. Bacteria found on washed and unwashed hand environments

ColonyColony SizeSize ShapeShape MarginMargin SurfaceSurface ColorColor

a.a. 8mm8mm IrregularIrregular SmoothSmooth SmoothSmooth YellowYellow

b.b. 8mm8mm RoundRound LobateLobate SmoothSmooth Clear to creamy Clear to creamy whitewhite

c.c. 3mm3mm RoundRound SmoothSmooth ConcentricConcentric WhiteWhite

d.d. 2mm2mm RoundRound LobateLobate SmoothSmooth Clear to off whiteClear to off white

e.e. 3mm3mm RoundRound LobateLobate Wrinkled and Wrinkled and smoothsmooth

ClearClear

f.f. Punctiform on e.Punctiform on e. RoundRound SmoothSmooth SmoothSmooth Slightly white to Slightly white to clearclear

g.g. 7mm7mm IrregularIrregular WavyWavy SmoothSmooth Creamy whiteCreamy white

Table 4. Bacteria colony morphology: unwashed hand

ColonyColony SizeSize ShapeShape MarginMargin SurfaceSurface ColorColor

a.a. 4mm4mm RoundRound LobateLobate SmoothSmooth Tan to whiteTan to white

b.b. 1mm1mm RoundRound SmoothSmooth SmoothSmooth OrangeOrange

c.c. 5mm5mm RoundRound SmoothSmooth SmoothSmooth Clear to off Clear to off whitewhite

d.d. 30mm30mm IrregularIrregular LobateLobate SmoothSmooth trasparenttrasparent

Table 6. Bacteria colony morphology: pond water

Fig 7. Bacteria found on pond water environment

ColonyColony SizeSize ShapeShape MarginMargin SurfaceSurface ColorColor

a.a. 3mm3mm RoundRound LobateLobate SmoothSmooth ClearClear

b.b. 3mm3mm RoundRound LobateLobate SmoothSmooth OrangeOrange

c.c. 10mm10mm RoundRound Smooth Smooth SmoothSmooth yellowyellow

d.d. 10mm10mm RoundRound SmoothSmooth SmoothSmooth whitewhite

e.e. 6mm6mm RoundRound WavyWavy MucoidalMucoidal whitewhite

Table 7. Bacteria colony morphology: Men’s bathroom door

Fig 8. Bacteria found on bathroom door environment

Side Study: Effect of Antibiotics on Side Study: Effect of Antibiotics on E. coli growthE. coli growth

AntibioticAntibiotic SensitivitySensitivity

Chloramphenicol-Chloramphenicol-30mg30mg

NSNS

Penicillin-10mgPenicillin-10mg NSNS

Nalidixic Acid-30mgNalidixic Acid-30mg VS*VS*

Norobiocin-30mgNorobiocin-30mg NSNS

Streptomycin-10mgStreptomycin-10mg VSVS

Erythromycin-15mgErythromycin-15mg SS

Tetracycline-30mgTetracycline-30mg VSVS

Kanamycin-30mgKanamycin-30mg VSVSFig 9. E coli growth among

antibiotics