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Successful Use of Corneal Tissue from LASIK Donors in Descemet-Stripping Automated Endothelial Keratoplasty: A Case Series Mark D. Mifflin, MD Majid Moshirfar, MD Yousuf M. Khalifa, MD JoAnn C. Chang, MD Carlton R. Fenzl, BS The John A. Moran Eye The John A. Moran Eye Center Center
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Successful Use of Corneal Tissue from LASIK Donors in Descemet-Stripping Automated Endothelial Keratoplasty: A Case Series Mark D. Mifflin, MD Majid Moshirfar,

Jan 19, 2016

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Page 1: Successful Use of Corneal Tissue from LASIK Donors in Descemet-Stripping Automated Endothelial Keratoplasty: A Case Series Mark D. Mifflin, MD Majid Moshirfar,

Successful Use of Corneal Tissue from LASIK Donors in Descemet-Stripping

Automated Endothelial Keratoplasty: A

Case Series

Mark D. Mifflin, MDMajid Moshirfar, MD

Yousuf M. Khalifa, MDJoAnn C. Chang, MDCarlton R. Fenzl, BS

The John A. Moran Eye CenterThe John A. Moran Eye Center

Page 2: Successful Use of Corneal Tissue from LASIK Donors in Descemet-Stripping Automated Endothelial Keratoplasty: A Case Series Mark D. Mifflin, MD Majid Moshirfar,

Financial Disclosure

• This project was supported in part by an unrestricted educational grant from Allergan Inc., Irvine, CA

• Research to Prevent Blindness (RPB), New York, NY

• Department of Ophthalmology and Visual Sciences at the University of Utah, John A. Moran Eye Center, Salt Lake City, UT

Page 3: Successful Use of Corneal Tissue from LASIK Donors in Descemet-Stripping Automated Endothelial Keratoplasty: A Case Series Mark D. Mifflin, MD Majid Moshirfar,

• Purpose: To report outcomes in Descemet’s stripping automated endothelial keratoplasty (DSAEK) using donor tissue from eyes that have had previous refractive surgery

• Methods: Retrospective case series. Preoperative and postoperative data were collected on each patient. Histopathological evaluation of two donor free caps were performed

Page 4: Successful Use of Corneal Tissue from LASIK Donors in Descemet-Stripping Automated Endothelial Keratoplasty: A Case Series Mark D. Mifflin, MD Majid Moshirfar,

Results

• 7 patients underwent DSAEK from donors that had undergone refractive surgery– One patient received PRK donor tissue, had no

interface haze– Six patients received LASIK donor tissue (Figure 1)

• Donor characterisitics: Table 1– Donor age ranged from 38 to 57– Precut pachymetry averaged 509 µm (range 460 to

575 µm)– Residual stromal bed (RSB) measured on average of

146 µm (RSB in one donor was not measurable)

Page 5: Successful Use of Corneal Tissue from LASIK Donors in Descemet-Stripping Automated Endothelial Keratoplasty: A Case Series Mark D. Mifflin, MD Majid Moshirfar,

Figure 1

Figure 1: Donor corneal button with previous LASIK in the viewing chamber. The black arrows indicate the flap edge. The white arrow is pointed to the hinge site.

Page 6: Successful Use of Corneal Tissue from LASIK Donors in Descemet-Stripping Automated Endothelial Keratoplasty: A Case Series Mark D. Mifflin, MD Majid Moshirfar,

Case Age (years)

Type of Refractive Procedure

Year of Refractive Procedure

ECD Death to Preservation

Precut Pachymetry

Microkeratome Head Size RSB Cap Thickness

Corneal Punch

Diameter

1 49 PRK 2007 2739 5h30m 487 300 Not Measurable * N/A 8.00

2 46 LASIK 2004 2590 13h 56m 525 300 155 370 8.00

3 46 LASIK 2004 2762 13h 56m 575 300 179 396 8.25

4 57 LASIK N/A 2857 8h 20m 500 350 110 390 8.00

5 44 LASIK 1997 2785 4h 35m 556 300 186 370 8.50

6 38 LASIK 2002 3105 16h 45m 460 300 120 340 8.00

7 38 LASIK 2002 2985 16h 45m 460 300 120 340 8.00

Case 2 and 3 received donor tissue from the same donor, and Case 6 and 7 received donor tissue from the same donor. * - ultrasound pachymetry was not able to generate a measurment of the RSB, ECD - endothelial cell density, RSB - residual stromal bed, PRK - photorefractive keratectomy, LASIK - laser in situ keratomileusis

Table 1: Donor Information

Page 7: Successful Use of Corneal Tissue from LASIK Donors in Descemet-Stripping Automated Endothelial Keratoplasty: A Case Series Mark D. Mifflin, MD Majid Moshirfar,

Results• Patient characteristics: Table 2

– Preoperative best-corrected visual acuity (BCVA) ranged from 20/40 to light perception

– Postoperative BCVA at 3 to 6 months ranged from 20/25 to 20/80

– One patient lost BCVA from 20/40 to 20/80 at 6 months • The patient had visually significant central donor macrofolds

– Endothelial cell density (ECD) were available for three patients between 3 to 11 months

• The average ECD in the 3 pts with available postoperative ECD was 1890 (SD 284) which represented a decrease of 33.1% (SD 10.5%) from preoperative values

Page 8: Successful Use of Corneal Tissue from LASIK Donors in Descemet-Stripping Automated Endothelial Keratoplasty: A Case Series Mark D. Mifflin, MD Majid Moshirfar,

Table 2: Patient Information

PBK - pseudophakic bullous keratopathy ECD - endothelial cell density BCVA - best corrected visual acuity DME - diabetic macular edema CME - cystoid macular edema RD - retinal detachment CF - count fingers * - Rigid Gas Permeable Contact Lens fitting

Case

Recipient

Sex Age (Years) Diagnosis Eye Preop ECD PreOp BCVA 1mo BCVA 3mo BCVA 6mo BCVA ECD

1 F 77 Fuch's OD unable 20/50 20/40- 20/50+ 20/25 1604 (11mo)

2 F 73 PBK OS Unable LP 20/400 20/200 20/30* N/A

3 M 68 PBK OD Unable CF @6' 20/400 CF @5' 20/40 2173 (6mo)

4 F 73 Fuch's OD unable 20/60 20/80 20/40 20/40 N/A

5 M 72 Fuch's OD 455 20/40- 20/80 20/80 20/80N/A

secondary to folds

6 M 72 Fuch's OD Unable 20/60 20/40 20/25 N/A N/A

7 F 67 Failed PKP OD Unable 20/150 20/40 20/25 N/A 1895 (3mo)

Page 9: Successful Use of Corneal Tissue from LASIK Donors in Descemet-Stripping Automated Endothelial Keratoplasty: A Case Series Mark D. Mifflin, MD Majid Moshirfar,

Results• Histological slides of two donor free caps are

shown in Figures 2 and 3. – In the case of one donor, the histologic slide reveals a

LASIK flap with uniform adherence to the underlying stroma.

– In the case of another donor, microscopic findings revealed that the LASIK flap remained adherent peripherally but showed a central detachment.

– We believe an artifact was elicited during the preparation of the microscopic slide since we did not encounter any flap related complications while preparing the endothelial button.

Page 10: Successful Use of Corneal Tissue from LASIK Donors in Descemet-Stripping Automated Endothelial Keratoplasty: A Case Series Mark D. Mifflin, MD Majid Moshirfar,

Results

Figure 2: Light photomicrograph of a donor free cap. The arrows show the adherence of the LASIK flap to the underlying stroma (magnification x100)

Figure 3: Light photomicrograph of a donor free cap. The left arrow shows adherence. The middle arrow shows separation of the LASIK flap from the underlying stroma. The right arrow indicates the flap edge (magnification x40)

Page 11: Successful Use of Corneal Tissue from LASIK Donors in Descemet-Stripping Automated Endothelial Keratoplasty: A Case Series Mark D. Mifflin, MD Majid Moshirfar,

Discussion• All patients underwent

uneventful DSAEK surgery with the exception of case 5 which had severe donor folds at the time of surgery that persisted despite a refloat/ reposition attempt postoperatively. – Etiology of folds uncertain.

Preparation of the graft was uneventful. The RSB of the donor graft measured186 µm. Intraoperative insertion was atraumatic.

Figure 4: Slit lamp photo of graft visually significant central macrofolds 1 month postoperatively. Note overall clarity and adherence of the donor graft.

Page 12: Successful Use of Corneal Tissue from LASIK Donors in Descemet-Stripping Automated Endothelial Keratoplasty: A Case Series Mark D. Mifflin, MD Majid Moshirfar,

Discussion

• Our initial findings are similar to previously reported cases of using DSAEK button with corneas that previously underwent LASIK¹·² which noted no significant difference in the preoperative preparation and postoperative results of endothelial keratoplasty using these donors.

• With the success and popularity of LASIK/PRK today, it will become more common to encounter donors who underwent this procedure. As the pool of LASIK altered eyes increase, it would be of interest if alterations of donor preparation (ie. positioning the flap hinge toward the microkeratome pass) would be necessary

1. Armour RL, Ousely PJ, Wall, J. et al. Endothelial Keratoplasty Using Donor Tissue Not Suitable for Full-Thickness Penetrating Keratoplasty. Cornea 2007; 26:515-519.

2. Phillips PM, Terry MA, Shamie N, et al. Descemet’s Stripping Automated Endothelial Keratoplasty (DSAEK) Using Corneal Donor Tissue Not Acceptable for Use in Penetrating Keratoplasty as a Result of Anterior Stromal Scars, Pterygia, and Previous Corneal Refractive Surgical Procedures. Cornea 2009; 28:871-876.