Proposed Syllabus and Structure For B.Sc. Programme with ...dhsgsu.ac.in/download/syllabus/BScIndustrialMicrobiology862018.pdfB.Sc. Programme with Industrial Microbiology (2 017-18)

B.Sc. Programme with Industrial Microbiology (2017-18)

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Proposed Syllabus and StructureFor

B.Sc. Programme with IndustrialMicrobiology

(2018-19)

Date of BoS: 08.06.18

Choice Based Credit System (CBCS)

-

Department of MicrobiologyDr. Harisingh Gour Vishwavidyalaya (A Central University)

Sagar (M.P.) 470003


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About the Department

The pioneering work done in the Microbiology and Mycology laboratory of the Botanydepartment triggered a vision of initiating a full-fledged curriculum in Applied Microbiology andBiotechnology at Dr. Harisingh Gour Vishwavidyalaya, Sagar. The idea conceived by thefounder head, Prof. S. C. Agrawal (Head:1996-2004) resulted in the start of this much awaitedPG course in Life Science faculty in 1990-91.

Under the able directios and untiring efforts of Late Prof. P.C. Jain (Head: 2004-2012) thedepartment could acquire the present status. Since then department has stood among the frontrunners in teaching and research in Microbiology and occupies a place of prominence in the field.In last ten years the department has witnessed a great deal of expansion with regards toinfrastructure and facilities as a seat of higher learning in Microbiology. With the establishment ofCentral University in 2009, the department was renamed as Department of Microbiology andbecame a part of School of Biological Sciences (SBS).The department offers following courses:

The department has published significant number of publications in the leading national andinternational peer reviewed scientific journals. In past the department has also organized severalhand’s on workshops, national and international conferences. At the department of Microbiologyresearch is being pursued in both basic and applied areas of Microbiology. With an inclinationtowards inter disciplinary research, faculty members in the department have forged activecollaboration within and outside the university. Recently, laboratories of bioimaging, infectionand immunity and host-pathogen interaction have been established. The major research fieldsencompass microbial hemicellulases, inulinases, L-asparaginase, thermophilic fungi,mycobacteria, host-pathogen interaction, enteric bacteria, bioimaging, biofilm and cancer biology.

S.No. Course Intake

1. B.Sc. with Industrial Microbiology 252. M.Sc. Microbiology 153. Ph.D. Microbiology 04

(subject to availability of seats)


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S.No. Course Intake




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S.No. Course Intake




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1. Name of the Program : B.Sc. Industrial Microbiology

2. Duration of the program:

(a) Minimum duration : 03 Years(b) Maximum duration: 05 Years

3. Scheme of examination

(a) Mid Semester Examination : 20 Marks(b) Internal Assessment : 20 Marks(c) End Semester Examination : 60 Marks

4. Assessment:

1) Internal Assessment:

a) TheoryEach theory course must clearly mention the methodology of assessment i.e.assignment, presentation, group discussion etc. depending on the number ofstudents in the class and feasibility of adopting a particular methodology. Thedistribution of marks of internal assessments shall be as follows:

(i) Evaluation of the assignmentPresentation, group discussion etc. : 15 marks

(ii) Attendance : 05 marks

The marks for attendance shall be awarded as follows:

(i) 75% and below : 00 Mark(ii) >75% and upto 80% : 01 Mark(iii) >80% and upto 85% : 02 Marks(iv) >85% and upto 90% : 03 Marks(v) >90% and upto 95% : 04 Marks(vi) >95% :05 Marks

Note: To be eligible to appear in End Semester Examination a student must appearin Mid Semester Examination and Internal Assessment.


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b) Practical/Lab Courses:

1. Evaluation of Practical/Lab Courses shall be as follows:

(i) Performing and getting the experiment checked regularly andIncorporating the suggestions in the practical note book : 15 Marks

(ii) Attendance : 05 Marks

The marks for attendance shall be as follows:

(i) 75% and below : 00 Mark(ii) >75% and upto 80% : 01 Mark(iii) >80% and upto 85% : 02 Marks(iv) >85% and upto 90% : 03 Marks(v) >90% and upto 95% : 04 Marks(vi) >95% :05 Marks

c) End Semester Examination for practical/Lab Courses:

It will consist of 60 marks as follows:(a) Assessment of performance in the Experiment : 50 Marks(b) Viva-Voce of Experiment : 10 Marks


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General Details of Courses and Credits Under Undergraduate Program (B.Sc.)

Courses Credits

Credits x No. of papers

I. Core Course CC (72 credits) 12 x 4 = 48

(12 Theory papers)04 Courses from each of the 03 disciplines

Core Course Practical/Tutorial*(12 Practical courses)04 Courses from each of the 03 Disciplines

12 × 2 = 24

II. Discipline Specific Elective (EC) (36 credits)(6 Papers)Two papers from each discipline of choice includingpaper of interdisciplinary nature.

6 × 4 = 24

Elective Course Practicals(6 Practical Courses)Two Papers from each discipline of choice includingpaper of interdisciplinary nature

6 × 2 = 12

III. Ability Enhancement Courses

1. Skill Enhancement Course (SE)(4 Papers of 2 credits each from 03 disciplines)

4 × 2 = 8

2. Ability Enhancement Compulsory(2 Papers of 2 credits each)Environmental ScienceEnglish/MIL Communication

2 × 2 = 4

Total credits= 120

Institute should evolve a system/ policy about ECA/ GeneralInterest/Hobby/Sports/NCC/NSS/related courses on its own.

*Wherever there is practical there will be no tutorials and vice-versa


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Proposed scheme for Choice Based Credit System BSc Program withMicrobiology

Semester Core Course CC (12) AbilityEnhancementCompulsoryCourse (2)(AECC)

SkillEnhancementCourse (2)

(MIC-SE)

Discipline Specific ElectiveCourse (6)

(MIC-EC)

1 Discipline-1Microbiology Paper I:Introduction toIndustrialMicrobiology andMicrobial DiversityMIC-CC-111MIC-CC-112

(English/MILCommunicatio/EnvironmentalScience)

CC-2

CC-3

2 Discipline-1Microbiology Paper II:EnvironmentalMicrobiologyMIC-CC-211MIC-CC-212

EnvironmentalScience/English/MILCommunication

CC-2

CC-33 Discipline-1

Microbiology PaperIII: BiochemistryMIC-CC-311MIC-CC-312

MIC-SE -311

CC-2

CC-3

4 Discipline-1Microbiology PaperIV: MicrobialPhysiology andMetabolismMIC-CC-411MIC-CC-412

MIC-SE -411

CC-2

CC-3

5 SE-3 MIC EC 511, MIC EC 512EC-Discipline Paper IEC-Discipline I

6 SE-4 MIC EC 611, MIC EC 612EC-Discipline Paper IIEC-Discipline II


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B.Sc. Syllabus (Industrial Microbiology)

1st Semester

Course No. Course Title CreditMIC CC 111 General Microbiology and Microbial Diversity

(Theory)04

MIC CC 112 General Microbiology and Microbial Diversity(Practical)

02

2nd Semester

MIC CC 211 Environmental Microbiology (Theory) 04MIC CC 212 Environmental Microbiology (Practical) 02

3rd Semester

MIC CC 311 Biochemistry (Theory) 04MIC CC 312 Biochemistry (Practical) 02MIC SE 311 Food Fermentation Technique 02

4th Semester

MIC CC 411 Microbial Physiology and Metabolism (Theory) 04MIC CC 412 Microbial Physiology and Metabolism (Practical) 02MIC SE 411 Microbial Diagnosis in Health Clinic 02

5th Semester

MIC EC 511 Microbial Biotechnology (Theory) 04MIC EC 512 Microbial Biotechnology (Practical) 02

6th Semester

MIC EC 611 Instrumentation and Biotechniques (Theory) 04MIC EC 612 Instrumentation and Biotechniques (Practical) 02

CC- Core Course, SE- Skill enhancement course, EC- Department Specific Elective


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Department of Microbiology

Class Subject Semester Course Code Course Title Marks Credit

B.Sc. IndustrialMicrobiology I MIC CC 111

General Microbiologyand Microbial Diversity

(Theory)Mid Sem 40End Sem 60

04

Unit 1 08 hoursHistory of Development of MicrobiologyDevelopment of microbiology as a discipline, Spontaneous generation vs. biogenesis. Contributions ofAnton von Leeuwenhoek, Louis Pasteur, Elie Metchnikoff, Edward Jenner Robert Koch, Joseph Lister,Alexander Fleming, Selman A.Waksman. Role of microorganisms in fermentation, Germ theory ofdisease, Development of various, golden era of microbiology, Establishment of fields of medicalmicrobiology and immunology through the work of Paul Ehrlich.

Unit 2 16 hoursMicrobial DiversitySystems of classification: Binomial Nomenclature, Whittaker’s five kingdom and Carl Woese’s threedomain classification systems and their utility. Difference between prokaryotic and eukaryoticmicroorganisms.Bacteriology: Bacteriology: Structure of different cell components of Eubacteria. Characteristicfeatures, classification and importance of Actinomycetes, Mycoplasma and Cyanobacteria.Mycology: Morphological features, classification (taxonomy) and characteristics of (Slime moulds)Myxomycetes (Slime moulds), Oomycetes, Zygomycetes, Ascomycetes, Basidiomycetes andDeuteromycetes.Virology: General Morphological features and ultrastructure of viruses; Classification of viruses.Related viral agents, viroids and prions. Life cycle of RNA and DNA viruses. Lytic cycle, Lysogeny;one step growth curve. Viroids, prions.

Unit 3 12 hoursIndustrial MicrobiologyConcept and scope. Nature and range of industrial products from microorganisms. Concept of industrialstrains, Strain development. A general knowledge of microbes used in production of food, dairy,chemicals (solvents), bioactive molecules (antibiotics; enzymes).

Unit 4 12 hoursTools and Techniques in MicrobiologyCulture media: preparation and types - defined, differential, selective and enrichment culture media.Isolation techniques: Pour plate, spread plate, streak plate, serial dilution method. Sterilization:.Principle and methods of sterilization, physical and chemical agents of sterilization; Disinfectants,Antiseptics, Phenol coefficient

Unit 5 12 hoursInstrumentation in MicrobiologyMicroscopy and microscopic techniques: Principles and application of light microscopy, dark field,phase contrast, fluorescence and electron microscopy. Micrometry, Camera lucida. Methods ofpurification of microbial cultures. Maintenance and preservation of microbial cultures, Lyophilization,Cryopreservation etc. Principle, working and applications of spectrophometer, pH meter.Centrifugation: types and applications, Design and use of fermenter.


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B.Sc. IndustrialMicrobiology I MIC CC 112

General Microbiology andMicrobial Diversity

(Practical)Mid Sem 40End Sem 60

02

60 Hours

List of Practicals

1. Microbiology Good Laboratory Practices and Biosafety.2. To study the principle and applications of important instruments (biological safety cabinets,autoclave, incubator, BOD incubator, hot air oven, light microscope, pH meter) used in themicrobiology laboratory.3. Preparation of culture media for bacterial cultivation.4. Sterilization of medium using Autoclave and assessment for sterility5. Sterilization of glassware using Hot Air Oven and assessment for sterility6. Sterilization of heat sensitive material by membrane filtration and assessment for sterility7. Demonstration of the presence of microflora in the environment by exposing nutrient agar

plates to air.8. Study of Rhizopus, Penicillium, Aspergillus using temporary mounts9. Gram’s Staining technique10. Effect of antibiotics and antiseptics.

Essential reading1. Tortora GJ, Funke BR and Case CL. (2008). Microbiology: An Introduction. 9th edition.PearsonEducation2.Wiley JM, Sherwood LM and Woolverton CJ. (2013) Prescott’s Microbiology. 9th Edition.McGrawHill International.3. Atlas RM. (1997). Principles of Microbiology. 2nd edition. WM.T.Brown Publishers.4. Pelczar MJ, Chan ECS and Krieg NR. (1993). Microbiology. 5th edition. McGrawHill Book Company.5. Stanier RY, Ingraham JL, Wheelis ML, and Painter PR. (2005). General Microbiology. 5thedition.McMillan.6. Naveen Kango (2013) Textbook of Microbiology, IK international publishers, New Delhi

Suggested Reading:1 Madigan MT, Martinko JM, Dunlap PV and Clark DP. (2014). Brock Biology of

Microorganisms.Pearson International Edition2. Cappucino J and Sherman N. (2010). Microbiology: A Laboratory Manual. 9th edition. Pearson

Education Limited


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B.Sc. IndustrialMicrobiology II MIC CC 211

EnvironmentalMicrobiology

(Theory)

Mid Sem 40End Sem 60

04

Unit 1 12 hoursHabitat EcologyTerrestrial Environment: Soil profile and soil microflora Aquatic Environment: Microflora of freshwater and marine habitats Atmosphere: Aeromicroflora and dispersal of microbes Extreme Habitats:Extremophiles: Microbes thriving at high & low temperatures, pH, high hydrostatic & osmoticpressures, salinity, & low nutrient levels. Microbial succession in decomposition of plant organicmatter

Unit 2 12 hoursMicrobial InteractionsMutualism, Synergism, Commensalism, Competition, Amensalism, Parasitism, Predation microbe-plant interaction: Symbiotic and non symbiotic interactions.

Unit 3 12 hoursWater MicrobiologySources of water. Distribution of microorganisms in water systems & water borne diseases.Treatment and safety of drinking (potable) water, methods to detect potability of water samples: (a)standard qualitative procedure: presumptive test/MPN analysis

Unit 4 12 hoursBiogeochemical CyclingCarbon cycle: Microbial degradation of cellulose, hemicelluloses, lignin and chitinNitrogen cycle: Nitrogen fixation, ammonification, nitrification, denitrification and nitrate reductionPhosphorus cycle: Phosphate immobilization and solubilisationSulphur cycle: Microbes involved in sulphur cycle

Unit 5 12 hours

Microbial BioremediationWaste Management: Sources and types of solid waste, Methods of solid waste disposal (compostingand sanitary landfill), Liquid waste management: Nature, Composition and strength of sewage(BOD and COD), Primary, secondary (oxidation ponds, trickling filter, activated sludge process andseptic tank) and tertiary sewage treatment. Principles and degradation of pesticides, organic(hydrocarbons, oil spills) and inorganic (Metals) matter, biosurfactants


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B.Sc. IndustrialMicrobiology II MIC CC 212

EnvironmentalMicrobiology

(Practical)


02

60 Hours

List of Practicals

1. Isolation & identification of microorganisms present in air using various air samplers.2. Check the efficiency of Laminar Flow & filtration techniques3. Isolation & identification of microorganisms present in Water systems.4. MPN analysis of tap water5. Enumeration of BOD of water samples6. Isolation of microbes (bacteria & fungi) from soil (28ºC & 45ºC).7. Isolation of microbes (bacteria & fungi) from rhizosphere and rhizoplane.

Essential reading

1. Textbook Of Microbiology by Dr Naveen Kango2. Prescott`Microbiology by Joanne Willey, Linda Sherwood and Christopher J. Woolverton3. Brock Biology of Microorganisms by Michael T. Madigan, John M. Martinko, Kelly S.Bender, Daniel H. Buckley, David A. Stahl and Thomas Brock4. Atlas RM and Bartha R. (2000). Microbial Ecology: Fundamentals & Applications. 4th edition.Benjamin/Cummings Science Publishing, USA5. Madigan MT, Martinko JM and Parker J. (2014). Brock Biology of Microorganisms. 14thedition. Pearson/ Benjamin Cummings6. Maier RM, Pepper IL and Gerba CP. (2009). Environmental Microbiology. 2nd edition,Academic Press7. Singh A, Kuhad, RC & Ward OP (2009). Advances in Applied Bioremediation. Volume 17,Springer-Verlag, Berlin Hedeilberg8. Coyne MS. (2001). Soil Microbiology: An Exploratory Approach. Delmar Thomson Learning.9. Basics of biostatics by Dhiraj Nagar

Suggested Reading:1. Okafor, N (2011). Environmental Microbiology of Aquatic & Waster systems. 1st edition,Springer, New York2. Barton LL & Northup DE (2011). Microbial Ecology. 1st edition, Wiley Blackwell, USACampbell RE. (1983). Microbial Ecology. Blackwell Scientific Publication, Oxford, England.


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B.Sc. IndustrialMicrobiology III MIC CC 311

Biochemistry(Theory)


04

Unit1 12 hoursBioenergeticsFirst and second laws of Thermodynamics. Definitions of Gibb’s Free Energy, enthalpy,and Entropyand mathematical relationship among them, Standard free energy change and equilibrium constant.

Unit 2 12 hoursCarbohydratesFamilies of monosaccharides: aldoses and ketoses, trioses, tetroses, pentoses, and hexoses. Stereoisomerism of monosaccharides, epimers, Mutarotation and anomers of glucose. Furanose and pyranoseforms of glucose and fructose, Sugar derivatives, glucosamine, galactosamine, muramic acid, N- acetylneuraminic acid, Disaccharides; concept of reducing and non-reducing sugars, Polysaccharides, storagepolysaccharides, starch and glycogen. Structural Polysaccharides, cellulose, peptidoglycan and chitin

Unit 3 12 hoursLipidsDefinition and major classes of storage and structural lipids. Storage lipids. Fatty acids structure andfunctions. Essential fatty acids. Triacyl glycerols structure, functions and properties. SaponificationStructural lipids. Phosphoglycerides: Building blocks, General structure, functions and properties.Structure of phosphatidylethanolamine and phosphatidylcholine, Sphingolipids: building blocks,

Unit 4 12 hours

Proteins `Functions of proteins, Primary structures of proteins: Amino acids, the building blocks of proteins.General formula of amino acid and concept of zwitterion.Classification, biochemical structure and notation of standard protein amino acids Ninhydrin reaction.Natural modifications of amino acids in proteins hydrolysine, cystine and hydroxyproline, Structure andfunctions of naturally occurring glutathione and insulin and synthetic aspartame, Secondary structure ofproteins: Peptide unit and its salient features. The alpha helix, the beta pleated sheet and their occurrencein proteins, Tertiary and quaternary structures of proteins.

Unit 5 12 hours

EnzymesStructure of enzyme: Apoenzyme and cofactors, prosthetic group-TPP, coenzyme, NAD, metal cofactors,Classification of enzymes, Mechanism of action of enzymes: active site, transition state complex andactivation energy. Lock and key hypothesis, and Induced Fit hypothesis. allosteric mechanism,Definitions of terms – enzyme unit, specific activity and turnover number.Multienzyme complex : lactate dehydrogenase, Effect of pH and temperature on enzyme activity. Enzymeinhibition: competitive, non-competitive.


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B.Sc. IndustrialMicrobiology III MIC CC 312

Biochemistry(Practical)


02

60 Hours

List of Practicals

1. Properties of water, Concept of pH and buffers, preparation of buffers and Numerical problemsto explain the concepts

2. Numerical problems on calculations of Standard Free Energy Change and Equilibrium constant3. Standard Free Energy Change of coupled reactions4. Qualitative/Quantitative tests for carbohydrates, reducing sugars, non reducing sugars5. Qualitative/Quantitative tests for lipids and proteins6. Study of protein secondary and tertiary structures with the help of models7. Study of enzyme kinetics – calculation of Vmax , Km, Kcat values8. Study effect of temperature, pH and Heavy metals on enzyme activity9. Estimation of any one vitamin

Essential reading

1. Campbell, MK (2012) Biochemistry, 7th ed., Published by Cengage Learning2. Campbell, PN and Smith AD (2011) Biochemistry Illustrated, 4th ed., Published by Churchill

Livingstone3. Berg JM, Tymoczko JL and Stryer L (2011) Biochemistry, W.H.Freeman and Company4. Nelson DL and Cox MM (2008) Lehninger Principles of Biochemistry, 5th Edition., W.H.

Freeman and Company,5. Willey MJ, Sherwood, LM & Woolverton C J (2013) Prescott, Harley and Klein’s

Microbiology by. 9th Ed., McGrawHill6. Voet,D. and Voet J.G (2004) Biochemistry 3rd edition, John Wiley and Sons,

Suggested Reading:1. Tymoczko JL, Berg JM and Stryer L (2012) Biochemistry: A short course, 2nd ed.,

W.H.Freeman2 MJ, Sherwood, LM & Woolverton C J (2013) Prescott, Harley and Klein’s Microbiology

by. 9th Ed., McGrawHill


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B.Sc. IndustrialMicrobiology III MIC SE 311

FoodFermentation

Technique


02

Unit 1 4 hoursFermented FoodsDefinition, types, advantages and health benefits.

Unit 2 8 hoursMilk Based Fermented FoodsDahi, Yogurt, Buttermilk (Chach) and cheese: Preparation of inoculums, types of microorganismsand production process

Unit 3 6 hoursGrain Based Fermented FoodsSoy sauce, Bread, Idli and Dosa: Microorganisms and production process.

Unit 4 4 hours

Vegetable Based Fermented FoodsPickels, Saeurkraut: Microorganisms and production process.

Unit 5 4 hours

Fermented Meat and FishTypes, microorganisms involved, fermentation process.

Unit 6 4 hoursPrebiotic, Probiotic (Synbiotic) FoodsDefinition, types, microorganisms and health benefits

Essential Readings1. Hui YH, Meunier-Goddik L, Josephsen J, Nip WK, Stanfield PS (2004) Handbook of food andfermentation technology, CRC Press2. Holzapfel W (2014) Advances in Fermented Foods and Beverages, Woodhead Publishing.

Suggested Readings1. Yadav JS, Grover, S and Batish VK (1993) A comprehensive dairy microbiology, Metropolitan2. Jay JM, Loessner MJ, Golden DA (2005) Modern Food Microbiology, 7th edition. Springer


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B.Sc. IndustrialMicrobiology IV MIC CC 411

Microbial Physiologyand Metabolism

(Theory)


04

Unit 1 12 hoursBiomembrane and Transport of soluteBiochemical properties of cell membrane. Difference between Archea and Eubacteria phospholipid.Diffusion gaseous exchange, Ficks law of diffusion, Osmosis, Plasmolysis Passive transport mechanism:structure and types of ion channel. Secondary active transport mechanism: Proton-pumps, Symport,Antiport and Uniport.

Unit 2 12 hoursPhotosynthetic MicrobesPhotosynthetic microbes: Oxygenic photosynthetic bacteria: concept of PSI and PSII, Z-scheme, Non-cyclic photophosphorylation. Anoxygenic photosynthetic bacteria: Cyclic photophosphorylation.Difference between photosynthesis of Purple and Filamentous green bacteria, Cyanobacteria and Greensulphur bacteria. Calvin cycle.

Unit 3 12 hoursCarbon and Energy MetabolismFundamentals pathways of Pyruvate formation (Embden-Meyerhof pathway (EMP) /glycolytic pathways,Pentose phosphate pathway (PPP) /hexose monophosphate shunt, Entner-Doudoroff pathway). TCA cycle.Electron transport chain (ETC): components of respiratory chain.

Unit 4 12 hours

Extremophiles PhysiologyMethanogenic bacteria their classification and the mechanism of CH4 production. Halophilic bacteria androle of purple membrane. Hydrogen oxidation bacteria.

Unit 5 12 hours

Nitrogen MetabolismIntroduction to biological nitrogen fixation, Ammonia assimilation. Assimilatory nitrate reduction,dissimilatory nitrate reduction, Nitrification and denitrification. Molecular adaptations in microbes fornitrogen fixation (free living aerobic, free living anaerobic and symbiotic microbes).


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B.Sc. IndustrialMicrobiology IV MIC CC 412

Microbial Physiologyand Metabolism

(Practical)


02

60 Hours

List of Practicals

1. Study and plot the growth curve of E. coli by turbidometric and standard plate count methods.2. Calculations of generation time and specific growth rate of bacteria from the graph plotted withthe given data3. Effect of temperature on growth of E. coli4. Effect of pH on growth of E. coli5. Effect of carbon and nitrogen sources on growth of E.coli6. Effect of salt on growth of E. coli7. Demonstration of alcoholic fermentation8. Demonstration of the thermal death time and decimal reduction time of E. coli.

Essential Readings

1. Madigan MT, and Martinko JM (2014). Brock Biology of Microorganisms. 14th edition.Prentice Hall International Inc.2. Moat AG and Foster JW. (2002). Microbial Physiology. 4th edition. John Wiley & Sons3. Reddy SR and Reddy SM. (2005). Microbial Physiology. Scientific Publishers India4. Stanier RY, Ingrahm JI, Wheelis ML and Painter PR. (1987). General Microbiology. 5thedition, McMillan Press.

Suggested Readings:1. Gottschalk G. (1986). Bacterial Metabolism. 2nd edition. Springer Verlag2. Wiley JM, Sherwood LM, and Woolverton CJ. (2013). Prescott’s Microbiology. 9th edition.

McGraw Hill Higher Education.


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B.Sc. IndustrialMicrobiology IV MIC SE 411 Microbial Diagnosis in

Health ClinicMid Sem 40End Sem 60

02

Unit 1 5 hoursImportance of Diagnosis of DiseasesBacterial, Viral, Fungal and Protozoan Diseases of various human body systems, Disease associatedclinical samples for diagnosis.Unit 2 5 hoursCollection of Clinical SamplesHow to collect clinical samples (oral cavity, throat, skin, Blood, CSF, urine and faeces) andprecautions required. Method of transport of clinical samples to laboratory and storage.

Unit 3 5 hoursDirect Microscopic Examination and Culture.Examination of sample by staining - Gram stain, & Giemsa stained thin blood film for malariaPreparation and use of culture media - Blood agar, Chocolate agar, Lowenstein-Jensen medium,MacConkey agar, distinct colony properties of various bacterial pathogens.Unit 4 5 hoursSerological and Molecular MethodsSerological Methods - Agglutination, ELISA, immunofluorescence, Nucleic acid based methods -PCR, Nucleic acid probes

Unit 5 10 hoursKits for Rapid Detection of PathogensTyphoid, Dengue & Blood group. Importance, Determination of resistance/sensitivity of bacteria usingdisc diffusion method, Determination of minimal inhibitory concentration (MIC) of an antibiotic by serialdouble dilution Method

List of Practicals1. Isolation of serum and plasma from blood samples, 2. Making of blood smear & staining3. Making of differential media for pathogenic microorganisms, 4. Isolation & identification ofmicroorganisms from skin, throat and nasal swab, 5. Determination of resistance/sensitivity ofbacteria using disc diffusion method, 6. Determination of minimal inhibitory concentration (MIC)of an antibiotic by serial double dilution method.

Essential Readings1. Ananthanarayan R and Paniker CKJ (2009)Textbook of Microbiology, 8th edition,

Universities Press Private Ltd.2. Brooks G.F., Carroll K.C., Butel J.S., Morse S.A. and Mietzner, T.A. (2013) Jawetz, Melnickand Adelberg’s Medical Microbiology. 26th edition. McGraw Hill Publication

Suggested reading1. Randhawa, VS, Mehta G and Sharma KB (2009) Practicals and Viva in Medical Microbiology2nd edition, Elsevier India Pvt Ltd2. Microbiology Including Immunology and Molecular Genetics. III Ed. By Davis.. Dulbecco,Eisen and Ginsberg.3. Medical Laboratory Manual for Tropical Countries. Vol. II by Cheesbrough, M.


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B.Sc. IndustrialMicrobiology V MIC EC 511 Microbial Biotechnology

(Theory)Mid Sem 40End Sem 60

04

Unit 1 14 hoursMicrobial Biotechnology and its ApplicationsMicrobial biotechnology: Scope and its applications in human therapeutics, agriculture (Biofertilizers,PGPR, Mycorrhizae), environmental, and food technologyUse of prokaryotic and eukaryotic microorganisms in biotechnological applicationsGenetically engineered microbes for industrial application: Bacteria and yeast

Unit 2 12 hoursTherapeutic and Industrial BiotechnologyRecombinant microbial production processes in pharmaceutical industries - Streptokinase,recombinant vaccines (Hepatitis B vaccine)Microbial polysaccharides and polyesters, Microbial production of bio-pesticides, bioplastics Microbialbiosensors

Unit 3 12 hoursApplications of Microbes in BiotransformationsMicrobial based transformation of steroids and sterolsBio-catalytic processes and their industrial applications: Production of high fructose syrup and productionof cocoa butter substitute

Unit 4 14 hours

Microbial Products and their RecoveryMicrobial product purification: filtration, ion exchange & affinity chromatography techniquesImmobilization methods and their application: Whole cell immobilization

Unit 5 8 hours

RNAiRNAi and its applications in silencing genes, drug resistance, therapeutics and host pathogenInteractions



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B.Sc. IndustrialMicrobiology V MIC EC 512

MicrobialBiotechnology

(Practical)


02

60 Hours

List of Practicals

1. Study yeast cell immobilization in calcium alginate gels2. Study enzyme immobilization by sodium alginate method3. Pigment production from fungi (Trichoderma / Aspergillus / Penicillium)4. Isolation of xylanase or lipase producing bacteria5. Study of algal Single Cell Proteins

Essential reading1. Ratledge, C and Kristiansen, B. (2001). Basic Biotechnology, 2nd Edition, CambridgeUniversity Press.2. Swartz, J. R. (2001). Advances in Escherichia coli production of therapeutic proteins. CurrentOpinion in Biotechnology, 12, 195–201.3. Prescott, Harley and Klein’s Microbiology by Willey JM, Sherwood LM, Woolverton CJ(2014), 9th edition, Mc Graw Hill Publishers.4. Gupta PK (2009) Elements of Biotechnology 2nd edition, Rastogi Publications,5. Glazer AN and Nikaido H (2007) Microbial Biotechnology, 2nd edition, Cambridge UniversityPress6. Glick BR, Pasternak JJ, and Patten CL (2010) Molecular Biotechnology 4th edition, ASMPress, 7. Crueger W, Crueger A (1990) Biotechnology: A text Book of Industrial Microbiology2nd edition Sinauer associates, Inc.

Suggested Reading

1. Demain, A. L and Davies, J. E. (1999). Manual of Industrial Microbiology and Biotechnology,2nd Edition, ASM Press.2. Stanbury PF, Whitaker A, Hall SJ (1995) Principles of Fermentation Technology 2nd edition.,Elsevier Science



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B.Sc. IndustrialMicrobiology VI MIC EC 611

Instrumentation andBiotechniques

(Theory)


04

Unit 1 10 hoursMicroscopyBrightfield and darkfield microscopy, Fluorescence Microscopy, Phase contrast Microscopy,Confocal Microscopy, Electron Microscopy (Scanning and Transmission Electron Microscopy) andMicrometry.

Unit 2 14 hoursChromatographyPrinciples and applications of paper chromatography (including Descending and 2-D), Thin layerchromatography. Column packing and fraction collection. Gel filtration chromatography, ionexchangechromatography and affinity chromatography, GLC, HPLC.

Unit 3 14 hoursElectrophoresisPrinciple and applications of native polyacrylamide gel electrophoresis, SDS- polyacrylamide gelelectrophoresis, 2D gel electrophoresis, Isoelectric focusing, Zymogram preparation and Agarose gelelectrophoresis.

Unit 4 10 hours

SpectrophotometryPrinciple and use of study of absorption spectra of biomolecules. Analysis of biomolecules using UVand visible range. Colorimetry and turbidometry.

Unit 5 12 hours

CentrifugationPreparative and analytical centrifugation, fixed angle and swinging bucket rotors. RCF andsedimentation coefficient, differential centrifugation, density gradient centrifugation andultracentrifugation.


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B.Sc. IndustrialMicrobiology VI MIC EC 612

Instrumentation andBiotechniques

(Practical)


02

60 Hours

List of Practicals

1. Study of fluorescent micrographs to visualize bacterial cells.2. Ray diagrams of phase contrast microscopy and Electron microscopy.3. Separation of mixtures by paper / thin layer chromatography.4. Demonstration of column packing in any form of column chromatography.5. Separation of protein mixtures by any form of chromatography.6. Separation of protein mixtures by Polyacrylamide Gel Electrophoresis (PAGE).7. Determination of λmax for an unknown sample and calculation of extinction coefficient.8. Separation of components of a given mixture using a laboratory scale centrifuge.9. Understanding density gradient centrifugation with the help of pictures.

Essential readings1. Wilson K and Walker J. (2010). Principles and Techniques of Biochemistry and MolecularBiology. 7th Ed., Cambridge University Press.2. Nelson DL and Cox MM. (2008). Lehninger Principles of Biochemistry, 5th Ed., W.H.Freeman and Company.3. Willey MJ, Sherwood LM & Woolverton C J. (2013). Prescott, Harley and Klein’sMicrobiology. 9thEd., McGraw Hill.4. Karp G. (2010) Cell and Molecular Biology: Concepts and Experiments. 6th edition. JohnWiley & Sons. Inc.5. De Robertis EDP and De Robertis EMF. (2006). Cell and Molecular Biology. 8th edition.Lipincott Williams and Wilkins, Philadelphia.

Suggested Readings:1. Cooper G.M. and Hausman R.E. (2009). The Cell: A Molecular Approach. 5th Edition.

ASM Press & Sunderland, Washington D.C., Sinauer Associates, MA.2. Nigam A and Ayyagari A. 2007. Lab Manual in Biochemistry, Immunology and

Biotechnology. Tata McGraw Hill.

Proposed Syllabus and Structure For B.Sc. Programme with ...dhsgsu.ac.in/download/syllabus/BScIndustrialMicrobiology862018.pdfB.Sc. Programme with Industrial Microbiology (2 017-18)

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