FORENSIC MEDICINE AND MEDICOLEGAL PAPER PRESIPITINE TEST Clilical Internship Forensic Medicine and Medicolegal Medical Faculty Diponegoro University / RSUP Dr. KARIADI SEMARANG NOVEMBER 7th- DECEMBER 3 rd , 2011
Oct 17, 2014
FORENSIC MEDICINE AND MEDICOLEGAL PAPER
PRESIPITINE TEST
Clilical Internship Forensic Medicine and MedicolegalMedical Faculty Diponegoro University / RSUP Dr.
KARIADI SEMARANGNOVEMBER 7th- DECEMBER 3rd, 2011
In the most physical criminal case such as murder, persecution, rape, et cetera, may be found blood, semen,
saliva, urine, hair and other body tissues in the crime scene
In the most physical criminal case such as murder, persecution, rape, et cetera, may be found blood, semen,
saliva, urine, hair and other body tissues in the crime scene
can be very useful to reveal the crime scientifically
can be very useful to reveal the crime scientifically
Among the various body fluids, blood is the
most important because it is a biological fluid with
more specific potential characteristic for certain
human groups
Among the various body fluids, blood is the
most important because it is a biological fluid with
more specific potential characteristic for certain
human groups
The main purpose of forensic blood test is actually to help identify the owner of the blood, by comparing the blood spots found at the crime scene or clothes from the victim or
the crime suspect
The main purpose of forensic blood test is actually to help identify the owner of the blood, by comparing the blood spots found at the crime scene or clothes from the victim or
the crime suspect
In this case, the investigator must obtain clarity on the three main
things, namely:
Benzidine or luminol test Precipitine test Agglutination test
Based on the description above, the problem identification is …
Whether there is precipitate ring on human blood that reacted
with rabbit serum?
BLOOD
Blood is the fluid that found in all living things (except plants) that serves to send a high level of substances and oxygen to the body tissues, carrying chemical products of
metabolism, as well as the body's defense against viruses or bacteria.
Antibodies
Antibodies or immunoglobulin (Ig) is a class of proteins
that formed plasma cells (B cell proliferation) due to contact with antigen.
Antibodies bind to specific antigens that create them.
Forensic Laboratory Examination
PREPARATION :Patches attached to
an object can be scraped off and
then soaked in physiological solution,or directly soaked with physiological sa
line solution when attach
ed to clothing
Theoretical FrameworkAnimal(Rabbit)
Treated group(injected with human blood )
Control group
Presipitine Test
Human Blood Identification
Materials :Human and animal (rabbit) blood 3cc and 1cc syringe, and 27 gauge needleAlcohol cotton 70%Test tubeCentrifugeNaCl
Method :Presipitin Test (specific test
to define to determine species)
Expected Result :Precipitate ring is formed
between anti-human serum and the extract, the ring is
muddy
Serum
Kelinci
Hari ke-
1 3 5 7 9 11
1 - - - + + -
2 - - - + + -
3 - - - + + -
4 - - - + + -
5 - - - - - -
6 - - - - - -
SUMMARY
• Blood forensic laboratory examination can be a valuable information or certain
criminal case in the court• All blood sample in this research showas
that precipitate ring is formed in day 7
• Blood forensic laboratory examination can be a valuable information or certain
criminal case in the court• All blood sample in this research showas
that precipitate ring is formed in day 7
SUGGESTION
• Put more attention to confusing variable of the research in order to
get more accurate result • Put more detail literature about physiology condition of the rabbit